FvH4_1g21280

ETHYLENE INSENSITIVE 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009800
Physical Location & Seq
Forward (+)
13283375 .. 13284724
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g21280.t1

Sequence Viewer

Length: 1245 bp
ATGGTAGAGATCGTTGAAGAAGTTGATCATCTAAGCTTGGAGGATGAAGAAGCTGAAGATATCAGTTACGAGGAGCTCAAGAAGCGAATGTGGAAGGATCGTGTGCGCTTAGAGAAGTTTAAGGAAATACGAAAAAGTGAAGAACCCGTGTCTTTGGCGAAAGAAGAGGCGTCTCGCCGGAAGAAGATGGCTAGAGCTCAAGATTCCATACTCAAATACATGGTGAAGATCATGGAAGTGTGCGAAGCACGAGGCTTCGTCTATGGGATCGTTCCGAAGAAGGGGAAGCCGGTGACAGGATCGTCGGATAGCTTACGAGAGTGGTGGAAAGACAACGTCCAGTTCGACCAGAACGCTCCACTTGCAATTTCCAAGTATCTACCAGAAGTGCTTTTGGAACAAGCATGTTCTAGTGGGCTGGATCAAGACAACTCTTATGTCCATCTCCTTCATGACTTGCAAGACACGACTCTGGGGTCGCTGCTTTCGGCTCTAATGCAACACTGCATACCTCCACAGAGGCGGTTTCCTTTGGAGAAAGGTTTGGCTCCACCGTGGTGGCCCAAGGGAAATGAGATATGGTGGGGCGACCAAGGGGTTTCTCAAGAGCATGGACCTCCTCCTTATAGGAAACCTCATGATCTCAAAAAGGCTTGGAAAGTAAGTGCTTTGGCTGCAGTGATTAAGCATATGGTTCCTAATATGGACAGGGTTAGAAGGCTAGTAAGGCAATCCAAGTCCTTGCAGGATAAAATGACTGCTAAGGAGGCCGCGATTTGGTCCAAAGTGAGAAAGTGTATGTTTGATGATGATGATGGCTCTGAATTGGATGCTCCAGTACATGTCTTCCCAAAAACTTCAAACAGTGAATGGGCTTTAGGCTTTGTGGATAAGAAATCGAGGGCAAGCCATAACGGAAAGAATGTTTCTGATACAACAGTGCGGGTTGACACAAGTTGCTCGTATGATCACTCCCCAATTGATTCAGAATTCGAAAGTGTGGTGGACTGGATTAATATGGAGCTACAAAAGGCCAGTGTAAACGAAGCTGGTGAAGTGGTGTCTGGTTGTCCAAGTGCATATTGGGAAGGTGTTATGGAAGATTCAGCTTTGGATGCAGCATTTGAGATTCAAAGGGAAAACATGGATTTAAATCTATATCCAGACGAAGGGATATCACAGATGAATCAAGAACCAACTTCAATTTGGGACTTGGGATATGATTATAACGATGCAAACTTGTAA

Protein Analysis

415

Amino Acids

47.25

Weight (kDa)

5.12

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIN3_DNA-bd PF04873 24 - 263 6.3e-114 Ethylene insensitive 3, DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1227
AasI GACNNNNNNGTC 2 cut(s) 301, 475
AccII CGCG 1 cut(s) 773
AciI CCGC 3 cut(s) 523, 771, 943
AclWI GGATC 4 cut(s) 105, 275, 307, 429
AcsI RAATTY 1 cut(s) 989
AcuI CTGAAG 1 cut(s) 75
AcyI GRCGYC 1 cut(s) 170
AfaI GTAC 1 cut(s) 840
AfiI CCNNNNNNNGG 4 cut(s) 281, 296, 777, 1169
AflIII ACRYGT 1 cut(s) 841
AgsI TTSAA 4 cut(s) 17, 861, 1133, 1203
AleI CACNNNNGTG 1 cut(s) 556
AluBI AGCT 8 cut(s) 36, 53, 76, 197, 312, 1024, 1049, 1109
AluI AGCT 8 cut(s) 36, 53, 76, 197, 312, 1024, 1049, 1109
Alw21I GWGCWC 2 cut(s) 78, 199
Alw26I GTCTC 1 cut(s) 177
AlwI GGATC 4 cut(s) 105, 275, 307, 429
AoxI GGCC 3 cut(s) 560, 768, 1032
ApeKI GCWGC 3 cut(s) 481, 674, 1118
ApoI RAATTY 1 cut(s) 989
AseI ATTAAT 1 cut(s) 1014
AspLEI GCGC 1 cut(s) 108
AspS9I GGNCC 3 cut(s) 561, 614, 780
AsuHPI GGTGA 3 cut(s) 235, 304, 1064
AsuII TTCGAA 1 cut(s) 993
AvaII GGWCC 2 cut(s) 614, 780
BaeI ACNNNNGTAYC 2 cut(s) 924, 957
BanII GRGCYC 2 cut(s) 78, 199
BauI CACGAG 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 838
Bbv12I GWGCWC 2 cut(s) 78, 199
BbvI GCAGC 3 cut(s) 468, 661, 1130
BccI CCATC 3 cut(s) 181, 450, 809
BclI TGATCA 2 cut(s) 25, 967
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 3 cut(s) 192, 411, 722
BfmI CTRYAG 1 cut(s) 675
BisI GCNGC 4 cut(s) 482, 675, 771, 1119
BlsI GCNGC 4 cut(s) 483, 676, 772, 1120
Bme18I GGWCC 2 cut(s) 614, 780
BmgT120I GGNCC 3 cut(s) 561, 614, 780
BmiI GGNNCC 2 cut(s) 549, 696
BmsI GCATC 3 cut(s) 820, 1105, 1222
BpiI GAAGAC 1 cut(s) 838
BpmI CTGGAG 1 cut(s) 819
Bpu10I CCTNAGC 1 cut(s) 762
Bpu14I TTCGAA 1 cut(s) 993
BpuEI CTTGAG 3 cut(s) 62, 183, 588
BsaBI GATNNNNATC 1 cut(s) 1152
BsaHI GRCGYC 1 cut(s) 170
BsaJI CCNNGG 3 cut(s) 554, 564, 592
Bsc4I CCNNNNNNNGG 4 cut(s) 281, 296, 777, 1169
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 4 cut(s) 340, 836, 1013, 1035
Bse8I GATNNNNATC 1 cut(s) 1152
BseDI CCNNGG 3 cut(s) 554, 564, 592
BseGI GGATG 3 cut(s) 49, 835, 1120
BseJI GATNNNNATC 1 cut(s) 1152
BseLI CCNNNNNNNGG 4 cut(s) 281, 296, 777, 1169
BseNI ACTGG 4 cut(s) 340, 836, 1013, 1035
BseRI GAGGAG 2 cut(s) 86, 609
BseXI GCAGC 3 cut(s) 468, 661, 1130
Bsh1236I CGCG 1 cut(s) 773
BshFI GGCC 3 cut(s) 562, 770, 1034
BsiHKAI GWGCWC 2 cut(s) 78, 199
BsiSI CCGG 2 cut(s) 178, 290
BslFI GGGAC 1 cut(s) 1223
BslI CCNNNNNNNGG 4 cut(s) 281, 296, 777, 1169
BsmAI GTCTC 1 cut(s) 177
BsmBI CGTCTC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 1223
BsnI GGCC 3 cut(s) 562, 770, 1034
Bsp119I TTCGAA 1 cut(s) 993
Bsp1286I GDGCHC 2 cut(s) 78, 199
Bsp143I GATC 9 cut(s) 9, 25, 97, 228, 267, 299, 421, 640, 967
BspACI CCGC 3 cut(s) 523, 771, 943
BspANI GGCC 3 cut(s) 562, 770, 1034
BspFNI CGCG 1 cut(s) 773
BspHI TCATGA 2 cut(s) 451, 637
BspLI GGNNCC 2 cut(s) 549, 696
BspMAI CTGCAG 1 cut(s) 679
BspPI GGATC 4 cut(s) 105, 275, 307, 429
BspT104I TTCGAA 1 cut(s) 993
BsrFI RCCGGY 1 cut(s) 289
BsrI ACTGG 4 cut(s) 340, 836, 1013, 1035
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 3 cut(s) 554, 564, 592
BssMI GATC 9 cut(s) 9, 25, 97, 228, 267, 299, 421, 640, 967
BssNI GRCGYC 1 cut(s) 170
BssSI CACGAG 1 cut(s) 249
BssT1I CCWWGG 2 cut(s) 564, 592
Bst2BI CACGAG 1 cut(s) 249
Bst4CI ACNGT 3 cut(s) 555, 866, 940
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 170
BstBI TTCGAA 1 cut(s) 993
BstC8I GCNNGC 1 cut(s) 907
BstDEI CTNAG 3 cut(s) 32, 109, 762
BstDSI CCRYGG 1 cut(s) 554
BstF5I GGATG 3 cut(s) 49, 835, 1120
BstFNI CGCG 1 cut(s) 773
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 9 cut(s) 12, 28, 100, 231, 270, 302, 424, 643, 970
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 9 cut(s) 9, 25, 97, 228, 267, 299, 421, 640, 967
BstMWI GCNNNNNNNGC 6 cut(s) 82, 362, 674, 727, 767, 1115
BstNSI RCATGY 2 cut(s) 408, 845
BstSFI CTRYAG 1 cut(s) 675
BstUI CGCG 1 cut(s) 773
BstV1I GCAGC 3 cut(s) 468, 661, 1130
BstV2I GAAGAC 1 cut(s) 838
BstXI CCANNNNNNTGG 1 cut(s) 558
BsuRI GGCC 3 cut(s) 562, 770, 1034
BtgI CCRYGG 1 cut(s) 554
BtsCI GGATG 3 cut(s) 49, 835, 1120
BtsI GCAGTG 2 cut(s) 502, 684
BtsIMutI CAGTG 5 cut(s) 502, 684, 871, 945, 1042
Cac8I GCNNGC 1 cut(s) 907
CciI TCATGA 2 cut(s) 451, 637
CfoI GCGC 1 cut(s) 108
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 3 cut(s) 561, 614, 780
CseI GACGC 1 cut(s) 159
Csp6I GTAC 1 cut(s) 839
CviAII CATG 8 cut(s) 220, 232, 405, 452, 611, 638, 842, 1144
CviQI GTAC 1 cut(s) 839
DdeI CTNAG 3 cut(s) 32, 109, 762
DpnI GATC 9 cut(s) 11, 27, 99, 230, 269, 301, 423, 642, 969
DpnII GATC 9 cut(s) 9, 25, 97, 228, 267, 299, 421, 640, 967
DraI TTTAAA 1 cut(s) 1152
DrdI GACNNNNNNGTC 2 cut(s) 301, 475
DseDI GACNNNNNNGTC 2 cut(s) 301, 475
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Ecl136II GAGCTC 2 cut(s) 76, 197
Eco130I CCWWGG 2 cut(s) 564, 592
Eco24I GRGCYC 2 cut(s) 78, 199
Eco32I GATATC 2 cut(s) 61, 1176
Eco47I GGWCC 2 cut(s) 614, 780
Eco53kI GAGCTC 2 cut(s) 76, 197
Eco57I CTGAAG 1 cut(s) 75
EcoICRI GAGCTC 2 cut(s) 76, 197
EcoRI GAATTC 1 cut(s) 989
EcoRV GATATC 2 cut(s) 61, 1176
EcoT14I CCWWGG 2 cut(s) 564, 592
EcoT38I GRGCYC 2 cut(s) 78, 199
ErhI CCWWGG 2 cut(s) 564, 592
Esp3I CGTCTC 1 cut(s) 177
FaeI CATG 8 cut(s) 223, 235, 408, 455, 614, 641, 845, 1147
FaqI GGGAC 1 cut(s) 1223
FatI CATG 8 cut(s) 219, 231, 404, 451, 610, 637, 841, 1143
FauI CCCGC 1 cut(s) 936
FauNDI CATATG 1 cut(s) 690
FbaI TGATCA 2 cut(s) 25, 967
Fnu4HI GCNGC 4 cut(s) 482, 675, 771, 1119
FokI GGATG 3 cut(s) 56, 842, 1127
FriOI GRGCYC 2 cut(s) 78, 199
Fsp4HI GCNGC 4 cut(s) 482, 675, 771, 1119
FspBI CTAG 3 cut(s) 192, 411, 722
GlaI GCGC 1 cut(s) 107
GluI GCNGC 4 cut(s) 482, 675, 771, 1119
GsuI CTGGAG 1 cut(s) 819
HaeIII GGCC 3 cut(s) 562, 770, 1034
HapII CCGG 2 cut(s) 178, 290
HgaI GACGC 1 cut(s) 159
HhaI GCGC 1 cut(s) 108
Hin1I GRCGYC 1 cut(s) 170
Hin1II CATG 8 cut(s) 223, 235, 408, 455, 614, 641, 845, 1147
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HincII GTYRAC 1 cut(s) 949
HindII GTYRAC 1 cut(s) 949
HindIII AAGCTT 1 cut(s) 34
HinfI GANTC 6 cut(s) 203, 469, 983, 1103, 1129, 1186
HpaII CCGG 2 cut(s) 178, 290
HphI GGTGA 3 cut(s) 235, 304, 1064
Hpy166II GTNNAC 3 cut(s) 949, 1006, 1042
Hpy188I TCNGA 5 cut(s) 276, 307, 823, 931, 988
Hpy188III TCNNGA 8 cut(s) 79, 200, 425, 452, 605, 638, 1163, 1190
Hpy8I GTNNAC 3 cut(s) 949, 1006, 1042
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 6 cut(s) 88, 274, 458, 711, 1082, 1163
HpyCH4III ACNGT 3 cut(s) 555, 866, 940
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 9 cut(s) 365, 460, 499, 507, 677, 745, 1079, 1118, 1235
HpyF10VI GCNNNNNNNGC 6 cut(s) 82, 362, 674, 727, 767, 1115
HpyF3I CTNAG 3 cut(s) 32, 109, 762
HpySE526I ACGT 1 cut(s) 336
Hsp92I GRCGYC 1 cut(s) 170
Hsp92II CATG 8 cut(s) 223, 235, 408, 455, 614, 641, 845, 1147
HspAI GCGC 1 cut(s) 106
Ksp22I TGATCA 2 cut(s) 25, 967
Kzo9I GATC 9 cut(s) 9, 25, 97, 228, 267, 299, 421, 640, 967
LmnI GCTCC 5 cut(s) 73, 361, 553, 838, 1021
Lsp1109I GCAGC 3 cut(s) 468, 661, 1130
LweI GCATC 3 cut(s) 820, 1105, 1222
MaeI CTAG 3 cut(s) 192, 411, 722
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 2 cut(s) 65, 292
MalI GATC 9 cut(s) 11, 27, 99, 230, 269, 301, 423, 642, 969
MboI GATC 9 cut(s) 9, 25, 97, 228, 267, 299, 421, 640, 967
MfeI CAATTG 1 cut(s) 978
MhlI GDGCHC 2 cut(s) 78, 199
MluCI AATT 5 cut(s) 366, 824, 978, 989, 1203
MlyI GAGTC 1 cut(s) 463
MmeI TCCRAC 1 cut(s) 285
MseI TTAA 4 cut(s) 120, 684, 1014, 1151
MslI CAYNNNNRTG 2 cut(s) 236, 556
MspI CCGG 2 cut(s) 178, 290
MunI CAATTG 1 cut(s) 978
MvnI CGCG 1 cut(s) 773
MwoI GCNNNNNNNGC 6 cut(s) 82, 362, 674, 727, 767, 1115
NdeI CATATG 1 cut(s) 690
NdeII GATC 9 cut(s) 9, 25, 97, 228, 267, 299, 421, 640, 967
NlaIII CATG 8 cut(s) 223, 235, 408, 455, 614, 641, 845, 1147
NlaIV GGNNCC 2 cut(s) 549, 696
NmuCI GTSAC 1 cut(s) 292
NspI RCATGY 2 cut(s) 408, 845
NspV TTCGAA 1 cut(s) 993
OliI CACNNNNGTG 1 cut(s) 556
PagI TCATGA 2 cut(s) 451, 637
PciI ACATGT 1 cut(s) 841
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PfeI GAWTC 5 cut(s) 203, 983, 1103, 1129, 1186
PflFI GACNNNGTC 1 cut(s) 335
PkrI GCNGC 4 cut(s) 483, 676, 772, 1120
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
PscI ACATGT 1 cut(s) 841
PshBI ATTAAT 1 cut(s) 1014
PsiI TTATAA 1 cut(s) 1227
Psp124BI GAGCTC 2 cut(s) 78, 199
PspN4I GGNNCC 2 cut(s) 549, 696
PspPI GGNCC 3 cut(s) 561, 614, 780
PstI CTGCAG 1 cut(s) 679
PsyI GACNNNGTC 1 cut(s) 335
RsaI GTAC 1 cut(s) 840
RsaNI GTAC 1 cut(s) 839
RseI CAYNNNNRTG 2 cut(s) 236, 556
SacI GAGCTC 2 cut(s) 78, 199
SaqAI TTAA 4 cut(s) 120, 684, 1014, 1151
SatI GCNGC 4 cut(s) 482, 675, 771, 1119
Sau3AI GATC 9 cut(s) 9, 25, 97, 228, 267, 299, 421, 640, 967
Sau96I GGNCC 3 cut(s) 561, 614, 780
SchI GAGTC 1 cut(s) 463
SduI GDGCHC 2 cut(s) 78, 199
SfaNI GCATC 3 cut(s) 820, 1105, 1222
SfcI CTRYAG 1 cut(s) 675
SfuI TTCGAA 1 cut(s) 993
SinI GGWCC 2 cut(s) 614, 780
SmiI ATTTAAAT 1 cut(s) 1152
SmiMI CAYNNNNRTG 2 cut(s) 236, 556
SmlI CTYRAG 3 cut(s) 77, 198, 603
SmoI CTYRAG 3 cut(s) 77, 198, 603
Sse9I AATT 5 cut(s) 366, 824, 978, 989, 1203
SsiI CCGC 3 cut(s) 523, 771, 943
SspMI CTAG 3 cut(s) 192, 411, 722
SstI GAGCTC 2 cut(s) 78, 199
StyI CCWWGG 2 cut(s) 564, 592
SwaI ATTTAAAT 1 cut(s) 1152
TaaI ACNGT 3 cut(s) 555, 866, 940
TaiI ACGT 1 cut(s) 339
TaqI TCGA 3 cut(s) 345, 899, 993
TasI AATT 5 cut(s) 366, 824, 978, 989, 1203
TatI WGTACW 1 cut(s) 838
TauI GCSGC 1 cut(s) 773
TfiI GAWTC 5 cut(s) 203, 983, 1103, 1129, 1186
Tru1I TTAA 4 cut(s) 120, 684, 1014, 1151
Tru9I TTAA 4 cut(s) 120, 684, 1014, 1151
TscAI CASTG 5 cut(s) 509, 684, 871, 945, 1042
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 3 cut(s) 481, 674, 1118
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 3 cut(s) 60, 440, 1199
TspGWI ACGGA 1 cut(s) 930
TspRI CASTG 5 cut(s) 509, 684, 871, 945, 1042
Tth111I GACNNNGTC 1 cut(s) 335
VpaK11BI GGWCC 2 cut(s) 614, 780
VspI ATTAAT 1 cut(s) 1014
XapI RAATTY 1 cut(s) 989
XceI RCATGY 2 cut(s) 408, 845
XcmI CCANNNNNNNNNTGG 2 cut(s) 1080, 1203
XspI CTAG 3 cut(s) 192, 411, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.