AT5G10750

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010107
Physical Location & Seq
Forward (+)
3398790 .. 3400281
1492 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10750.1

Sequence Viewer

Length: 909 bp
ATGAGCCCTTCCAAGCAGCGACATCGTAGCTCTACCGGAGAAAACAAGTCAAAGCCGGTCAGATCCGCTTCATCCTCCGCCATACCGGAATGGATTACCGAATCAACCAACGGCGGATCTCTCCGTCGCGTAGATCCCGATACAGGTACCGACGGCTGGGCTTCTCCTCCCGGCGATGTTTTCTCTCTCCGCTCCGATTCTTACTTGTCCAAAAAACAAAAAACTCCCGCCGGTGATTACCTCCTTTCTCCCGCCGGCATGGACTGGCTCAAATCAAGTACCAAACTCGAGAACGCGCTCGCTCGTCCCGATAACCGTGTAGCTCACGCGCTTAGAAAAGCTCAATCTCGCGGTCAATCTCTCAAGAGCTTCATCTTCGCCGTGAATCTCCAGATTCCAGGTAAGGATCATCACAGCGCCGTGTTCTACTTCGCGACGGAAGAACCGATTCCTTCCGGTTCGCTTCTCCACCGGTTCATCAACGGCGACGATGCCTTCCGGAACCAGAGATTCAAAATCGTGAATCGGATTGTGAAAGGTCCTTGGGTAGTTAAAGCCGCGGTGGGGAACTATAGCGCTTGTCTTCTAGGTAAAGCCTTGACGTGCAATTACCACAGAGGTCCTAACTACTTTGAAATTGACGTCGACATTAGCAGCTCAGCGATCGCAACGGCGATTCTACGGCTGGCTTTAGGGTACGTGACGAGCGTGACAATCGATATGGGGTTCTTAGCAGAGGCACAAACGGAAGAGGAGCTGCCGGAGAGATTAATCGGAGCTGTTAGAGTTTGCCAAATGGAGATGTCGTCAGCGTTTGTTGTCGACGCGCCACCACCGCAGCAACTGCCGTCACAACCGTGTAGAACATTAAGTTCGGCGAAAGTTAACCATGACGAGGACGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

302

Amino Acids

33.02

Weight (kDa)

8.29

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 53 - 267 2.7e-79 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 645
Acc65I GGTACC 1 cut(s) 146
AccB1I GGYRCC 1 cut(s) 146
AccBSI CCGCTC 1 cut(s) 192
AccI GTMKAC 2 cut(s) 645, 822
AccII CGCG 7 cut(s) 129, 296, 329, 351, 434, 560, 827
AccIII TCCGGA 1 cut(s) 498
AclWI GGATC 4 cut(s) 57, 124, 128, 414
AcyI GRCGYC 1 cut(s) 642
AfaI GTAC 3 cut(s) 148, 280, 698
AfeI AGCGCT 1 cut(s) 577
AfiI CCNNNNNNNGG 4 cut(s) 143, 156, 564, 895
AgeI ACCGGT 1 cut(s) 471
AgsI TTSAA 2 cut(s) 514, 635
AjiI CACGTC 1 cut(s) 603
AjnI CCWGG 1 cut(s) 397
AleI CACNNNNGTG 1 cut(s) 856
AluBI AGCT 7 cut(s) 30, 323, 341, 369, 657, 757, 779
AluI AGCT 7 cut(s) 30, 323, 341, 369, 657, 757, 779
AlwI GGATC 4 cut(s) 57, 124, 128, 414
AlwNI CAGNNNCTG 1 cut(s) 844
Ama87I CYCGRG 1 cut(s) 287
Aor13HI TCCGGA 1 cut(s) 498
Aor51HI AGCGCT 1 cut(s) 577
ApeKI GCWGC 4 cut(s) 16, 654, 757, 838
AseI ATTAAT 1 cut(s) 770
AsiGI ACCGGT 1 cut(s) 471
AsiSI GCGATCGC 1 cut(s) 666
Asp700I GAANNNNTTC 1 cut(s) 447
Asp718I GGTACC 1 cut(s) 146
AspLEI GCGC 5 cut(s) 298, 331, 419, 578, 829
AspS9I GGNCC 2 cut(s) 539, 620
AsuC2I CCSGG 1 cut(s) 171
AsuHPI GGTGA 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 2 cut(s) 539, 620
BanI GGYRCC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 575
BbvI GCAGC 4 cut(s) 28, 666, 744, 850
BceAI ACGGC 8 cut(s) 127, 169, 365, 404, 499, 687, 698, 832
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 1 cut(s) 399
BcnI CCSGG 1 cut(s) 171
BfaI CTAG 1 cut(s) 587
BfmI CTRYAG 1 cut(s) 571
BfoI RGCGCY 2 cut(s) 420, 579
BisI GCNGC 5 cut(s) 17, 558, 655, 758, 839
BlpI GCTNAGC 1 cut(s) 658
BlsI GCNGC 5 cut(s) 18, 559, 656, 759, 840
Bme1390I CCNGG 2 cut(s) 171, 399
Bme18I GGWCC 2 cut(s) 539, 620
BmeT110I CYCGRG 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 603
BmgT120I GGNCC 2 cut(s) 539, 620
BmiI GGNNCC 2 cut(s) 148, 503
BmrFI CCNGG 2 cut(s) 171, 399
BmsI GCATC 1 cut(s) 481
BpiI GAAGAC 1 cut(s) 575
BpmI CTGGAG 1 cut(s) 374
Bpu1102I GCTNAGC 1 cut(s) 658
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 171
Bsa29I ATCGAT 1 cut(s) 717
BsaAI YACGTR 1 cut(s) 700
BsaHI GRCGYC 1 cut(s) 642
BsaJI CCNNGG 2 cut(s) 542, 558
BsaWI WCCGGW 5 cut(s) 35, 85, 455, 471, 498
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 156, 564, 895
Bse118I RCCGGY 4 cut(s) 55, 230, 254, 471
Bse1I ACTGG 1 cut(s) 269
BseAI TCCGGA 1 cut(s) 498
BseBI CCWGG 1 cut(s) 399
BseCI ATCGAT 1 cut(s) 717
BseDI CCNNGG 2 cut(s) 542, 558
BseGI GGATG 1 cut(s) 71
BseLI CCNNNNNNNGG 4 cut(s) 143, 156, 564, 895
BseMII CTCAG 1 cut(s) 672
BseNI ACTGG 1 cut(s) 269
BseRI GAGGAG 2 cut(s) 156, 767
BseXI GCAGC 4 cut(s) 28, 666, 744, 850
BseYI CCCAGC 1 cut(s) 156
Bsh1236I CGCG 7 cut(s) 129, 296, 329, 351, 434, 560, 827
Bsh1285I CGRYCG 1 cut(s) 666
BshNI GGYRCC 1 cut(s) 146
BshTI ACCGGT 1 cut(s) 471
BshVI ATCGAT 1 cut(s) 717
BsiEI CGRYCG 1 cut(s) 666
BsiHKCI CYCGRG 1 cut(s) 287
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 4 cut(s) 143, 156, 564, 895
BsmFI GGGAC 1 cut(s) 291
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 1 cut(s) 8
Bsp13I TCCGGA 1 cut(s) 498
Bsp143I GATC 5 cut(s) 62, 116, 133, 406, 663
Bsp1720I GCTNAGC 1 cut(s) 658
Bsp68I TCGCGA 1 cut(s) 434
BspCNI CTCAG 1 cut(s) 671
BspDI ATCGAT 1 cut(s) 717
BspEI TCCGGA 1 cut(s) 498
BspFNI CGCG 7 cut(s) 129, 296, 329, 351, 434, 560, 827
BspLI GGNNCC 2 cut(s) 148, 503
BspPI GGATC 4 cut(s) 57, 124, 128, 414
BspT107I GGYRCC 1 cut(s) 146
BsrBI CCGCTC 1 cut(s) 192
BsrFI RCCGGY 4 cut(s) 55, 230, 254, 471
BsrI ACTGG 1 cut(s) 269
BssAI RCCGGY 4 cut(s) 55, 230, 254, 471
BssECI CCNNGG 2 cut(s) 542, 558
BssMI GATC 5 cut(s) 62, 116, 133, 406, 663
BssNI GRCGYC 1 cut(s) 642
BssT1I CCWWGG 1 cut(s) 542
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 2 cut(s) 317, 858
Bst6I CTCTTC 1 cut(s) 744
BstACI GRCGYC 1 cut(s) 642
BstAPI GCANNNNNTGC 1 cut(s) 844
BstBAI YACGTR 1 cut(s) 700
BstC8I GCNNGC 3 cut(s) 256, 300, 687
BstDEI CTNAG 3 cut(s) 332, 658, 730
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 1 cut(s) 71
BstFNI CGCG 7 cut(s) 129, 296, 329, 351, 434, 560, 827
BstH2I RGCGCY 2 cut(s) 420, 579
BstHHI GCGC 5 cut(s) 298, 331, 419, 578, 829
BstKTI GATC 5 cut(s) 65, 119, 136, 409, 666
BstMBI GATC 5 cut(s) 62, 116, 133, 406, 663
BstMCI CGRYCG 1 cut(s) 666
BstMWI GCNNNNNNNGC 2 cut(s) 835, 844
BstNI CCWGG 1 cut(s) 399
BstSCI CCNGG 2 cut(s) 169, 397
BstSFI CTRYAG 1 cut(s) 571
BstUI CGCG 7 cut(s) 129, 296, 329, 351, 434, 560, 827
BstV1I GCAGC 4 cut(s) 28, 666, 744, 850
BstV2I GAAGAC 1 cut(s) 575
BstX2I RGATCY 3 cut(s) 62, 116, 133
BstYI RGATCY 3 cut(s) 62, 116, 133
Bsu15I ATCGAT 1 cut(s) 717
BsuTUI ATCGAT 1 cut(s) 717
BtgI CCRYGG 1 cut(s) 558
BtgZI GCGATG 1 cut(s) 189
BtrI CACGTC 1 cut(s) 603
BtsCI GGATG 1 cut(s) 71
BtuMI TCGCGA 1 cut(s) 434
Cac8I GCNNGC 3 cut(s) 256, 300, 687
CaiI CAGNNNCTG 1 cut(s) 844
CfoI GCGC 5 cut(s) 298, 331, 419, 578, 829
Cfr10I RCCGGY 4 cut(s) 55, 230, 254, 471
Cfr13I GGNCC 2 cut(s) 539, 620
Cfr42I CCGCGG 1 cut(s) 561
ClaI ATCGAT 1 cut(s) 717
CseI GACGC 1 cut(s) 833
Csp6I GTAC 3 cut(s) 147, 279, 697
CspAI ACCGGT 1 cut(s) 471
CviAII CATG 2 cut(s) 259, 890
CviQI GTAC 3 cut(s) 147, 279, 697
DdeI CTNAG 3 cut(s) 332, 658, 730
DpnI GATC 5 cut(s) 64, 118, 135, 408, 665
DpnII GATC 5 cut(s) 62, 116, 133, 406, 663
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
EciI GGCGGA 2 cut(s) 67, 129
Eco130I CCWWGG 1 cut(s) 542
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 2 cut(s) 539, 620
Eco47III AGCGCT 1 cut(s) 577
Eco88I CYCGRG 1 cut(s) 287
EcoO109I RGGNCCY 2 cut(s) 539, 620
EcoRII CCWGG 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 542
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 1 cut(s) 542
FaeI CATG 2 cut(s) 262, 893
FaiI YATR 5 cut(s) 83, 260, 573, 722, 891
FaqI GGGAC 1 cut(s) 291
FatI CATG 2 cut(s) 258, 889
FauI CCCGC 2 cut(s) 235, 259
FblI GTMKAC 2 cut(s) 645, 822
Fnu4HI GCNGC 5 cut(s) 17, 558, 655, 758, 839
FokI GGATG 1 cut(s) 58
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 5 cut(s) 17, 558, 655, 758, 839
FspBI CTAG 1 cut(s) 587
GlaI GCGC 5 cut(s) 297, 330, 418, 577, 828
GluI GCNGC 5 cut(s) 17, 558, 655, 758, 839
GsaI CCCAGC 1 cut(s) 160
GsuI CTGGAG 1 cut(s) 374
HaeII RGCGCY 2 cut(s) 420, 579
HgaI GACGC 1 cut(s) 833
HhaI GCGC 5 cut(s) 298, 331, 419, 578, 829
Hin1I GRCGYC 1 cut(s) 642
Hin1II CATG 2 cut(s) 262, 893
Hin6I GCGC 5 cut(s) 296, 329, 417, 576, 827
HinP1I GCGC 5 cut(s) 296, 329, 417, 576, 827
HincII GTYRAC 3 cut(s) 646, 823, 886
HindII GTYRAC 3 cut(s) 646, 823, 886
HinfI GANTC 8 cut(s) 101, 197, 385, 394, 448, 510, 523, 676
HpaI GTTAAC 1 cut(s) 886
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 3 cut(s) 646, 823, 886
Hpy188I TCNGA 4 cut(s) 62, 196, 528, 776
Hpy188III TCNNGA 8 cut(s) 137, 289, 308, 364, 391, 433, 499, 520
Hpy8I GTNNAC 3 cut(s) 646, 823, 886
Hpy99I CGWCG 6 cut(s) 129, 155, 439, 491, 647, 827
HpyAV CCTTC 3 cut(s) 18, 462, 505
HpyCH4III ACNGT 2 cut(s) 317, 858
HpyCH4IV ACGT 3 cut(s) 602, 642, 699
HpyCH4V TGCA 1 cut(s) 606
HpyF10VI GCNNNNNNNGC 2 cut(s) 835, 844
HpyF3I CTNAG 3 cut(s) 332, 658, 730
HpySE526I ACGT 3 cut(s) 602, 642, 699
Hsp92I GRCGYC 1 cut(s) 642
Hsp92II CATG 2 cut(s) 262, 893
HspAI GCGC 5 cut(s) 296, 329, 417, 576, 827
Kpn2I TCCGGA 1 cut(s) 498
KpnI GGTACC 1 cut(s) 150
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
KspAI GTTAAC 1 cut(s) 886
KspI CCGCGG 1 cut(s) 561
Kzo9I GATC 5 cut(s) 62, 116, 133, 406, 663
LmnI GCTCC 3 cut(s) 197, 754, 776
Lsp1109I GCAGC 4 cut(s) 28, 666, 744, 850
LweI GCATC 1 cut(s) 481
MaeI CTAG 1 cut(s) 587
MaeII ACGT 3 cut(s) 602, 642, 699
MaeIII GTNAC 3 cut(s) 700, 709, 849
MalI GATC 5 cut(s) 64, 118, 135, 408, 665
MbiI CCGCTC 1 cut(s) 192
MboI GATC 5 cut(s) 62, 116, 133, 406, 663
MboII GAAGA 4 cut(s) 367, 452, 575, 761
MflI RGATCY 3 cut(s) 62, 116, 133
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 2 cut(s) 607, 636
MnlI CCTC 8 cut(s) 85, 177, 251, 611, 730, 745, 889, 895
MroI TCCGGA 1 cut(s) 498
MroNI GCCGGC 1 cut(s) 254
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 4 cut(s) 552, 770, 869, 885
MslI CAYNNNNRTG 1 cut(s) 856
MspA1I CMGCKG 1 cut(s) 560
MspR9I CCNGG 2 cut(s) 171, 399
MvaI CCWGG 1 cut(s) 399
MvnI CGCG 7 cut(s) 129, 296, 329, 351, 434, 560, 827
MwoI GCNNNNNNNGC 2 cut(s) 835, 844
NaeI GCCGGC 1 cut(s) 256
NciI CCSGG 1 cut(s) 171
NdeII GATC 5 cut(s) 62, 116, 133, 406, 663
NgoMIV GCCGGC 1 cut(s) 254
NlaIII CATG 2 cut(s) 262, 893
NlaIV GGNNCC 2 cut(s) 148, 503
NmuCI GTSAC 3 cut(s) 700, 709, 849
NruI TCGCGA 1 cut(s) 434
OliI CACNNNNGTG 1 cut(s) 856
PaeR7I CTCGAG 1 cut(s) 287
PcsI WCGNNNNNNNCGW 3 cut(s) 306, 443, 705
PdiI GCCGGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 8 cut(s) 101, 197, 385, 394, 448, 510, 523, 676
PinAI ACCGGT 1 cut(s) 471
PkrI GCNGC 5 cut(s) 18, 559, 656, 759, 840
Ple19I CGATCG 1 cut(s) 666
Ppu21I YACGTR 1 cut(s) 700
PpuMI RGGWCCY 2 cut(s) 539, 620
PshBI ATTAAT 1 cut(s) 770
Psp5II RGGWCCY 2 cut(s) 539, 620
Psp6I CCWGG 1 cut(s) 397
PspFI CCCAGC 1 cut(s) 156
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 2 cut(s) 148, 503
PspPI GGNCC 2 cut(s) 539, 620
PspPPI RGGWCCY 2 cut(s) 539, 620
PstNI CAGNNNCTG 1 cut(s) 844
PsuI RGATCY 3 cut(s) 62, 116, 133
PvuI CGATCG 1 cut(s) 666
RgaI GCGATCGC 1 cut(s) 666
RruI TCGCGA 1 cut(s) 434
RsaI GTAC 3 cut(s) 148, 280, 698
RsaNI GTAC 3 cut(s) 147, 279, 697
RseI CAYNNNNRTG 1 cut(s) 856
SacII CCGCGG 1 cut(s) 561
SalI GTCGAC 2 cut(s) 644, 821
SaqAI TTAA 4 cut(s) 552, 770, 869, 885
SatI GCNGC 5 cut(s) 17, 558, 655, 758, 839
Sau3AI GATC 5 cut(s) 62, 116, 133, 406, 663
Sau96I GGNCC 2 cut(s) 539, 620
ScrFI CCNGG 2 cut(s) 171, 399
SduI GDGCHC 1 cut(s) 8
SfaAI GCGATCGC 1 cut(s) 666
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 1 cut(s) 571
Sfr274I CTCGAG 1 cut(s) 287
Sfr303I CCGCGG 1 cut(s) 561
SgfI GCGATCGC 1 cut(s) 666
SgrAI CRCCGGYG 1 cut(s) 230
SgrBI CCGCGG 1 cut(s) 561
SinI GGWCC 2 cut(s) 539, 620
SlaI CTCGAG 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 856
SmlI CTYRAG 2 cut(s) 287, 362
SmoI CTYRAG 2 cut(s) 287, 362
Sse9I AATT 2 cut(s) 607, 636
SspMI CTAG 1 cut(s) 587
StyD4I CCNGG 2 cut(s) 169, 397
StyI CCWWGG 1 cut(s) 542
TaaI ACNGT 2 cut(s) 317, 858
TaiI ACGT 3 cut(s) 605, 645, 702
TaqI TCGA 4 cut(s) 288, 645, 717, 822
TasI AATT 2 cut(s) 607, 636
TauI GCSGC 1 cut(s) 560
TfiI GAWTC 8 cut(s) 101, 197, 385, 394, 448, 510, 523, 676
Tru1I TTAA 4 cut(s) 552, 770, 869, 885
Tru9I TTAA 4 cut(s) 552, 770, 869, 885
TseFI GTSAC 3 cut(s) 700, 709, 849
TseI GCWGC 4 cut(s) 16, 654, 757, 838
Tsp45I GTSAC 3 cut(s) 700, 709, 849
TspDTI ATGAA 3 cut(s) 60, 361, 466
TspGWI ACGGA 3 cut(s) 113, 452, 761
VpaK11BI GGWCC 2 cut(s) 539, 620
VspI ATTAAT 1 cut(s) 770
XhoI CTCGAG 1 cut(s) 287
XmiI GTMKAC 2 cut(s) 645, 822
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 1 cut(s) 587
ZraI GACGTC 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.