MD15G1044400.v1.1

Protein ENHANCED DISEASE RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010107
Physical Location & Seq
Reverse (-)
3052096 .. 3053514
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1044400.v1.1.491

Sequence Viewer

Length: 918 bp
ATGTGTCCGACGAAACAAAAGCACCGGAGCTCCGTCGCGGAACACGCCGGAAAATGCTCCAGATCGGAGGATTCTACAACCTCCACCGCCCACTGGATCTCCGAGTCCGTCCACGGCGGATCCTTGCGCCACGTGGACCTCACCACCGGAACCAACGGCTGGGCGTCACCTCCCGGGGACCTATTCTCGCTCCGCGCCAAGAACTACTTGTCGAAAAAACAAAAAGGCCCCGCCGGCGACTACCTACTGCAGCCGTGCGGCACTGACTGGCTCAGATCCAGCACGAAACTCGAGAACGTACTCGCTCGCCCCGATAATCGCGTGGCCAACGCGCTTCGGAAGGCACAGTCGCAAGGGAGGTCCCTGAAGAGCTTCATTTTCGCCGTGAATCTCCAGATTCCCGGCAAGGACCAGCACAGCGCCGTTTTCTATTTCGCCACGGAGGATCCTATCCCTGCCGGCTCGCTTCTCTACCGGTTCGTCAACGGCGACGACGCGTTCCGGAACCAGCGGTTCAAGATCGTGAACCGGATCGTGAAAGGGCCGTGGATCGTCGAGAAGACGGTAGGGAATTACAGTGCGTGCTTGCTGGGGAAGGCGCTGACGTGTAATTACCACAGAGGACCCAACTACCTCGAGATTGACGTCGATATCGCGAGTTTTGGGATCGCGAAAGCGATTCTGCGACTTGCATTGAGGTACGTGACGAGCGTGACGATCGACATGGGGTTTGTGGTGGAGGCGCAGGCGGAGGATGAGCTGCCTGAGAAGTTAGTCGGCGCGGTTCGGGTATGCGAGATGGAGATGTTGTCTGCAACGGTCGTGGAGGCGCCGCAGACGACGGTCGTAGCGCGGGGATTGAGTTTTGCGTCTAAGGTGAATCATCACAAGTCCGGCGACGACGACGACGACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

306

Amino Acids

33.45

Weight (kDa)

8.45

Isoelectric Point (pI)

34.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 54 - 268 1.4e-77 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 829
AccB7I CCANNNNNTGG 1 cut(s) 159
AccII CGCG 9 cut(s) 38, 195, 321, 332, 497, 656, 671, 782, 853
AccIII TCCGGA 1 cut(s) 501
AclWI GGATC 9 cut(s) 104, 114, 127, 270, 440, 453, 539, 557, 674
AcoI YGGCCR 1 cut(s) 324
AcuI CTGAAG 1 cut(s) 386
AcvI CACGTG 1 cut(s) 133
AcyI GRCGYC 3 cut(s) 164, 645, 830
AfaI GTAC 2 cut(s) 300, 701
AfiI CCNNNNNNNGG 2 cut(s) 93, 159
AflIII ACRYGT 2 cut(s) 495, 605
AgeI ACCGGT 1 cut(s) 474
AgsI TTSAA 1 cut(s) 517
AjiI CACGTC 1 cut(s) 606
AluBI AGCT 3 cut(s) 30, 372, 760
AluI AGCT 3 cut(s) 30, 372, 760
Alw21I GWGCWC 1 cut(s) 32
AlwI GGATC 9 cut(s) 104, 114, 127, 270, 440, 453, 539, 557, 674
Ama87I CYCGRG 3 cut(s) 173, 290, 635
Aor13HI TCCGGA 1 cut(s) 501
AoxI GGCC 3 cut(s) 226, 324, 542
ApeKI GCWGC 2 cut(s) 250, 760
ArsI GACNNNNNNTTYG 2 cut(s) 713, 745
AsiGI ACCGGT 1 cut(s) 474
Asp700I GAANNNNTTC 1 cut(s) 371
AspLEI GCGC 9 cut(s) 129, 197, 334, 422, 601, 745, 782, 832, 853
AspS9I GGNCC 7 cut(s) 136, 178, 227, 360, 409, 542, 623
AsuC2I CCSGG 3 cut(s) 174, 175, 402
AsuHPI GGTGA 3 cut(s) 133, 159, 889
AvaI CYCGRG 3 cut(s) 173, 290, 635
AvaII GGWCC 5 cut(s) 136, 178, 360, 409, 623
BalI TGGCCA 1 cut(s) 326
BamHI GGATCC 2 cut(s) 119, 445
BanI GGYRCC 1 cut(s) 829
BanII GRGCYC 1 cut(s) 32
BbrPI CACGTG 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 566
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 262, 747
BccI CCATC 1 cut(s) 793
BceAI ACGGC 7 cut(s) 130, 172, 238, 368, 407, 502, 529
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BcnI CCSGG 3 cut(s) 174, 175, 402
BfmI CTRYAG 1 cut(s) 248
BfoI RGCGCY 3 cut(s) 423, 602, 833
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 4 cut(s) 251, 259, 761, 833
BlsI GCNGC 4 cut(s) 252, 260, 762, 834
Bme1390I CCNGG 3 cut(s) 174, 175, 402
Bme18I GGWCC 5 cut(s) 136, 178, 360, 409, 623
BmeT110I CYCGRG 3 cut(s) 173, 290, 635
BmgBI CACGTC 1 cut(s) 606
BmgT120I GGNCC 7 cut(s) 136, 178, 227, 360, 409, 542, 623
BmiI GGNNCC 9 cut(s) 121, 151, 179, 229, 362, 447, 506, 625, 831
BmrFI CCNGG 3 cut(s) 174, 175, 402
BoxI GACNNNNGTC 1 cut(s) 842
BpiI GAAGAC 1 cut(s) 566
BpmI CTGGAG 2 cut(s) 43, 377
BpuMI CCSGG 3 cut(s) 174, 175, 402
BsaAI YACGTR 2 cut(s) 133, 703
BsaHI GRCGYC 3 cut(s) 164, 645, 830
BsaJI CCNNGG 5 cut(s) 112, 173, 174, 438, 545
BsaWI WCCGGW 5 cut(s) 24, 146, 474, 501, 528
BsaXI ACNNNNNCTCC 6 cut(s) 14, 44, 83, 113, 794, 824
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 159
Bse118I RCCGGY 3 cut(s) 233, 458, 474
Bse1I ACTGG 2 cut(s) 98, 272
BseAI TCCGGA 1 cut(s) 501
BseDI CCNNGG 5 cut(s) 112, 173, 174, 438, 545
BseGI GGATG 1 cut(s) 760
BseLI CCNNNNNNNGG 2 cut(s) 93, 159
BseMII CTCAG 2 cut(s) 286, 756
BseNI ACTGG 2 cut(s) 98, 272
BseXI GCAGC 2 cut(s) 262, 747
BseYI CCCAGC 2 cut(s) 159, 589
Bsh1236I CGCG 9 cut(s) 38, 195, 321, 332, 497, 656, 671, 782, 853
Bsh1285I CGRYCG 3 cut(s) 720, 822, 846
BshFI GGCC 3 cut(s) 228, 326, 544
BshNI GGYRCC 1 cut(s) 829
BshTI ACCGGT 1 cut(s) 474
BsiEI CGRYCG 3 cut(s) 720, 822, 846
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 3 cut(s) 173, 290, 635
BslFI GGGAC 2 cut(s) 191, 346
BslI CCNNNNNNNGG 2 cut(s) 93, 159
BsmFI GGGAC 2 cut(s) 191, 346
BsnI GGCC 3 cut(s) 228, 326, 544
BsoBI CYCGRG 3 cut(s) 173, 290, 635
Bsp1286I GDGCHC 1 cut(s) 32
Bsp13I TCCGGA 1 cut(s) 501
Bsp68I TCGCGA 2 cut(s) 656, 671
BspANI GGCC 3 cut(s) 228, 326, 544
BspCNI CTCAG 2 cut(s) 285, 757
BspEI TCCGGA 1 cut(s) 501
BspFNI CGCG 9 cut(s) 38, 195, 321, 332, 497, 656, 671, 782, 853
BspLI GGNNCC 9 cut(s) 121, 151, 179, 229, 362, 447, 506, 625, 831
BspMAI CTGCAG 1 cut(s) 252
BspPI GGATC 9 cut(s) 104, 114, 127, 270, 440, 453, 539, 557, 674
BspQI GCTCTTC 1 cut(s) 362
BspT107I GGYRCC 1 cut(s) 829
BsrFI RCCGGY 3 cut(s) 233, 458, 474
BsrI ACTGG 2 cut(s) 98, 272
BssAI RCCGGY 3 cut(s) 233, 458, 474
BssECI CCNNGG 5 cut(s) 112, 173, 174, 438, 545
BssNI GRCGYC 3 cut(s) 164, 645, 830
Bst4CI ACNGT 5 cut(s) 348, 565, 578, 820, 844
Bst6I CTCTTC 1 cut(s) 362
BstACI GRCGYC 3 cut(s) 164, 645, 830
BstBAI YACGTR 2 cut(s) 133, 703
BstC8I GCNNGC 7 cut(s) 235, 307, 460, 464, 583, 587, 747
BstDEI CTNAG 3 cut(s) 272, 765, 873
BstDSI CCRYGG 3 cut(s) 112, 438, 545
BstF5I GGATG 1 cut(s) 760
BstFNI CGCG 9 cut(s) 38, 195, 321, 332, 497, 656, 671, 782, 853
BstH2I RGCGCY 3 cut(s) 423, 602, 833
BstHHI GCGC 9 cut(s) 129, 197, 334, 422, 601, 745, 782, 832, 853
BstMCI CGRYCG 3 cut(s) 720, 822, 846
BstMWI GCNNNNNNNGC 2 cut(s) 44, 234
BstPAI GACNNNNGTC 1 cut(s) 842
BstSCI CCNGG 3 cut(s) 172, 173, 400
BstSFI CTRYAG 1 cut(s) 248
BstUI CGCG 9 cut(s) 38, 195, 321, 332, 497, 656, 671, 782, 853
BstV1I GCAGC 2 cut(s) 262, 747
BstV2I GAAGAC 1 cut(s) 566
BstX2I RGATCY 4 cut(s) 96, 119, 275, 445
BstYI RGATCY 4 cut(s) 96, 119, 275, 445
BsuRI GGCC 3 cut(s) 228, 326, 544
BtgI CCRYGG 3 cut(s) 112, 438, 545
BtrI CACGTC 1 cut(s) 606
BtsCI GGATG 1 cut(s) 760
BtsIMutI CAGTG 3 cut(s) 91, 261, 583
BtuMI TCGCGA 2 cut(s) 656, 671
Cac8I GCNNGC 7 cut(s) 235, 307, 460, 464, 583, 587, 747
CfoI GCGC 9 cut(s) 129, 197, 334, 422, 601, 745, 782, 832, 853
Cfr10I RCCGGY 3 cut(s) 233, 458, 474
Cfr13I GGNCC 7 cut(s) 136, 178, 227, 360, 409, 542, 623
Cfr9I CCCGGG 1 cut(s) 173
CseI GACGC 3 cut(s) 153, 503, 858
Csp6I GTAC 2 cut(s) 299, 700
CspAI ACCGGT 1 cut(s) 474
CspCI CAANNNNNGTGG 2 cut(s) 804, 839
CviAII CATG 1 cut(s) 724
CviQI GTAC 2 cut(s) 299, 700
DdeI CTNAG 3 cut(s) 272, 765, 873
DinI GGCGCC 1 cut(s) 831
EaeI YGGCCR 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
EciI GGCGGA 2 cut(s) 132, 764
Ecl136II GAGCTC 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 32
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 5 cut(s) 136, 178, 360, 409, 623
Eco53kI GAGCTC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 386
Eco72I CACGTG 1 cut(s) 133
Eco88I CYCGRG 3 cut(s) 173, 290, 635
EcoICRI GAGCTC 1 cut(s) 30
EcoO109I RGGNCCY 4 cut(s) 178, 227, 360, 623
EcoRV GATATC 1 cut(s) 652
EcoT38I GRGCYC 1 cut(s) 32
EgeI GGCGCC 1 cut(s) 831
EheI GGCGCC 1 cut(s) 831
FaeI CATG 1 cut(s) 727
FaiI YATR 2 cut(s) 725, 793
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FaqI GGGAC 2 cut(s) 191, 346
FatI CATG 1 cut(s) 723
FauI CCCGC 2 cut(s) 238, 846
Fnu4HI GCNGC 4 cut(s) 251, 259, 761, 833
FokI GGATG 1 cut(s) 767
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 4 cut(s) 251, 259, 761, 833
GlaI GCGC 9 cut(s) 128, 196, 333, 421, 600, 744, 781, 831, 852
GluI GCNGC 4 cut(s) 251, 259, 761, 833
GsaI CCCAGC 2 cut(s) 163, 593
GsuI CTGGAG 2 cut(s) 43, 377
HaeII RGCGCY 3 cut(s) 423, 602, 833
HaeIII GGCC 3 cut(s) 228, 326, 544
HgaI GACGC 3 cut(s) 153, 503, 858
HhaI GCGC 9 cut(s) 129, 197, 334, 422, 601, 745, 782, 832, 853
Hin1I GRCGYC 3 cut(s) 164, 645, 830
Hin1II CATG 1 cut(s) 727
Hin6I GCGC 9 cut(s) 127, 195, 332, 420, 599, 743, 780, 830, 851
HinP1I GCGC 9 cut(s) 127, 195, 332, 420, 599, 743, 780, 830, 851
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HinfI GANTC 6 cut(s) 71, 104, 388, 397, 679, 880
HphI GGTGA 3 cut(s) 133, 159, 889
Hpy166II GTNNAC 4 cut(s) 112, 136, 484, 526
Hpy188I TCNGA 5 cut(s) 9, 67, 103, 275, 339
Hpy8I GTNNAC 4 cut(s) 112, 136, 484, 526
HpyAV CCTTC 2 cut(s) 334, 589
HpyCH4III ACNGT 5 cut(s) 348, 565, 578, 820, 844
HpyCH4IV ACGT 5 cut(s) 132, 297, 605, 645, 702
HpyCH4V TGCA 3 cut(s) 250, 692, 815
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 234
HpyF3I CTNAG 3 cut(s) 272, 765, 873
HpySE526I ACGT 5 cut(s) 132, 297, 605, 645, 702
Hsp92I GRCGYC 3 cut(s) 164, 645, 830
Hsp92II CATG 1 cut(s) 727
HspAI GCGC 9 cut(s) 127, 195, 332, 420, 599, 743, 780, 830, 851
KasI GGCGCC 1 cut(s) 829
Kpn2I TCCGGA 1 cut(s) 501
KroI GCCGGC 2 cut(s) 233, 458
KroNI GCCGGC 2 cut(s) 235, 460
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 4 cut(s) 27, 35, 62, 195
Lsp1109I GCAGC 2 cut(s) 262, 747
MaeII ACGT 5 cut(s) 132, 297, 605, 645, 702
MaeIII GTNAC 3 cut(s) 165, 703, 712
MboII GAAGA 2 cut(s) 379, 571
MflI RGATCY 4 cut(s) 96, 119, 275, 445
MhlI GDGCHC 1 cut(s) 32
MlsI TGGCCA 1 cut(s) 326
MluCI AATT 2 cut(s) 571, 610
MluI ACGCGT 1 cut(s) 495
MluNI TGGCCA 1 cut(s) 326
Mly113I GGCGCC 1 cut(s) 830
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 32
Mox20I TGGCCA 1 cut(s) 326
MreI CGCCGGCG 1 cut(s) 233
MroI TCCGGA 1 cut(s) 501
MroNI GCCGGC 2 cut(s) 233, 458
MroXI GAANNNNTTC 1 cut(s) 371
MscI TGGCCA 1 cut(s) 326
Msp20I TGGCCA 1 cut(s) 326
MspA1I CMGCKG 1 cut(s) 511
MspR9I CCNGG 3 cut(s) 174, 175, 402
MvnI CGCG 9 cut(s) 38, 195, 321, 332, 497, 656, 671, 782, 853
MwoI GCNNNNNNNGC 2 cut(s) 44, 234
NaeI GCCGGC 2 cut(s) 235, 460
NarI GGCGCC 1 cut(s) 830
NciI CCSGG 3 cut(s) 174, 175, 402
NgoMIV GCCGGC 2 cut(s) 233, 458
NlaIII CATG 1 cut(s) 727
NlaIV GGNNCC 9 cut(s) 121, 151, 179, 229, 362, 447, 506, 625, 831
NmuCI GTSAC 3 cut(s) 165, 703, 712
NruI TCGCGA 2 cut(s) 656, 671
PaeR7I CTCGAG 2 cut(s) 290, 635
PciSI GCTCTTC 1 cut(s) 362
PcsI WCGNNNNNNNCGW 7 cut(s) 309, 486, 642, 708, 713, 906, 909
PdiI GCCGGC 2 cut(s) 235, 460
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 5 cut(s) 71, 388, 397, 679, 880
PflMI CCANNNNNTGG 1 cut(s) 159
PinAI ACCGGT 1 cut(s) 474
PkrI GCNGC 4 cut(s) 252, 260, 762, 834
Ple19I CGATCG 1 cut(s) 720
PleI GAGTC 1 cut(s) 112
PluTI GGCGCC 1 cut(s) 833
PmaCI CACGTG 1 cut(s) 133
PmlI CACGTG 1 cut(s) 133
PpsI GAGTC 1 cut(s) 112
Ppu21I YACGTR 2 cut(s) 133, 703
PpuMI RGGWCCY 3 cut(s) 178, 360, 623
PshAI GACNNNNGTC 1 cut(s) 842
Psp124BI GAGCTC 1 cut(s) 32
Psp5II RGGWCCY 3 cut(s) 178, 360, 623
PspCI CACGTG 1 cut(s) 133
PspFI CCCAGC 2 cut(s) 159, 589
PspN4I GGNNCC 9 cut(s) 121, 151, 179, 229, 362, 447, 506, 625, 831
PspPI GGNCC 7 cut(s) 136, 178, 227, 360, 409, 542, 623
PspPPI RGGWCCY 3 cut(s) 178, 360, 623
PstI CTGCAG 1 cut(s) 252
PsuI RGATCY 4 cut(s) 96, 119, 275, 445
PvuI CGATCG 1 cut(s) 720
RruI TCGCGA 2 cut(s) 656, 671
RsaI GTAC 2 cut(s) 300, 701
RsaNI GTAC 2 cut(s) 299, 700
SacI GAGCTC 1 cut(s) 32
SapI GCTCTTC 1 cut(s) 362
SatI GCNGC 4 cut(s) 251, 259, 761, 833
Sau96I GGNCC 7 cut(s) 136, 178, 227, 360, 409, 542, 623
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 3 cut(s) 174, 175, 402
SduI GDGCHC 1 cut(s) 32
SfcI CTRYAG 1 cut(s) 248
SfoI GGCGCC 1 cut(s) 831
Sfr274I CTCGAG 2 cut(s) 290, 635
SgrAI CRCCGGYG 1 cut(s) 233
SinI GGWCC 5 cut(s) 136, 178, 360, 409, 623
SlaI CTCGAG 2 cut(s) 290, 635
SmaI CCCGGG 1 cut(s) 175
SmlI CTYRAG 2 cut(s) 290, 635
SmoI CTYRAG 2 cut(s) 290, 635
Sse9I AATT 2 cut(s) 571, 610
SspDI GGCGCC 1 cut(s) 829
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 3 cut(s) 172, 173, 400
TaaI ACNGT 5 cut(s) 348, 565, 578, 820, 844
TaiI ACGT 5 cut(s) 135, 300, 608, 648, 705
TaqI TCGA 6 cut(s) 212, 291, 555, 636, 648, 720
TasI AATT 2 cut(s) 571, 610
TauI GCSGC 2 cut(s) 261, 835
TfiI GAWTC 5 cut(s) 71, 388, 397, 679, 880
TscAI CASTG 3 cut(s) 98, 268, 583
TseFI GTSAC 3 cut(s) 165, 703, 712
TseI GCWGC 2 cut(s) 250, 760
Tsp45I GTSAC 3 cut(s) 165, 703, 712
TspDTI ATGAA 1 cut(s) 364
TspGWI ACGGA 3 cut(s) 22, 97, 455
TspMI CCCGGG 1 cut(s) 173
TspRI CASTG 3 cut(s) 98, 268, 583
Van91I CCANNNNNTGG 1 cut(s) 159
VpaK11BI GGWCC 5 cut(s) 136, 178, 360, 409, 623
XhoI CTCGAG 2 cut(s) 290, 635
XmaI CCCGGG 1 cut(s) 173
XmnI GAANNNNTTC 1 cut(s) 371
ZraI GACGTC 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.