MD08G1050500.v1.1

Protein ENHANCED DISEASE RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010107
Physical Location & Seq
Forward (+)
3923162 .. 3924073
912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1050500.v1.1.491

Sequence Viewer

Length: 912 bp
ATGTGTCCAACAAAGCAAAAGCACCGGAGCTCCGTCGCCGACCACGCAGGAAAATGCTCCGAATCGGAGGATTCTACAACCTCCACCGCCGACTGGATCTCCGAGTCCGTCCACGGCGGATCCTTGCGCCACGTGGACCTCACCACGGGAACCAACGGCTGGGCGTCCCCTCCCGGCGACCTATTCTCCCTCCGCACCAAGAAATACTTGTCGAATAAACAAAAAGGCTCCGCCGGCGACTACCTACTCCAGCCGTGCGGCACAGACTGGCTCAGATCCAGCACGAAACTCGATAACGTACTCGCCCGCCCCGATAATCGCGTGGCCAACGCGCTGCGGAAGGCTCAGTCGCAAGGGAAGTCGATGAAGAGCTTCATTTTCGCCGTGAATCTCCAGATTCCCGGCAAGGACCAGCACAGCGCGGTTTTCTACTTCGCGGCGGAGGATCCAATCCCCGCCGGCTCTCTCCTCTACCGGTTCATCCACGGCGACGACGCGTTCCGGAATCAGCGGTTCAAGATCGTGAACCGGATCGTGAAGGGGCCGTGGATCGTCGTGAAGACGGTGGGGAATTACAGTGCGTGTTTGCTGGGGAAGGCGCTGACTTGTAATTACCACAGAGGACCCAACTACCTCGAGATTGACGTCGATATCGCGAGCTCGGGGATCGCGAAGGCCATTTTACGACTCGCATTGAGGTACGTGACGAGCGTGGCGATAGACATGGGGTTTGTTGTGGAGGCGCAGGCGGAGGATGAGCTACCAGAAAAGTTAGTCGGCGCGGTTAGGGTATGCCAGATGGAGATGTCGTCGGCGACGGTCGTGGACGCGCCGCAGATCGTAGCGCGTGGATTGAGTTTTGCGTCGATGTTGAATCATCATAAGTCCGGCGACGATGACGACGACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

304

Amino Acids

33.06

Weight (kDa)

7.65

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 54 - 268 4.4e-76 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 648
AccB7I CCANNNNNTGG 1 cut(s) 159
AccIII TCCGGA 1 cut(s) 501
AclWI GGATC 9 cut(s) 104, 114, 127, 270, 440, 453, 539, 557, 674
AcoI YGGCCR 1 cut(s) 324
AcvI CACGTG 1 cut(s) 133
AcyI GRCGYC 2 cut(s) 164, 645
AfaI GTAC 2 cut(s) 300, 701
AfiI CCNNNNNNNGG 3 cut(s) 93, 145, 159
AflIII ACRYGT 1 cut(s) 495
AgeI ACCGGT 1 cut(s) 474
AgsI TTSAA 2 cut(s) 517, 874
AluBI AGCT 4 cut(s) 30, 372, 660, 760
AluI AGCT 4 cut(s) 30, 372, 660, 760
Alw21I GWGCWC 2 cut(s) 32, 662
AlwI GGATC 9 cut(s) 104, 114, 127, 270, 440, 453, 539, 557, 674
Ama87I CYCGRG 2 cut(s) 635, 661
Aor13HI TCCGGA 1 cut(s) 501
AoxI GGCC 3 cut(s) 324, 542, 675
ApeKI GCWGC 1 cut(s) 334
ArsI GACNNNNNNTTYG 2 cut(s) 713, 745
AsiGI ACCGGT 1 cut(s) 474
Asp700I GAANNNNTTC 1 cut(s) 371
AspLEI GCGC 8 cut(s) 129, 334, 422, 601, 745, 782, 832, 847
AspS9I GGNCC 4 cut(s) 136, 409, 542, 623
AsuC2I CCSGG 2 cut(s) 174, 402
AsuHPI GGTGA 1 cut(s) 133
AvaI CYCGRG 2 cut(s) 635, 661
AvaII GGWCC 3 cut(s) 136, 409, 623
BalI TGGCCA 1 cut(s) 326
BamHI GGATCC 2 cut(s) 119, 445
BanII GRGCYC 2 cut(s) 32, 662
BbrPI CACGTG 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 566
Bbv12I GWGCWC 2 cut(s) 32, 662
BbvI GCAGC 1 cut(s) 321
BccI CCATC 1 cut(s) 793
BceAI ACGGC 6 cut(s) 130, 172, 238, 368, 502, 529
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BcnI CCSGG 2 cut(s) 174, 402
BfoI RGCGCY 1 cut(s) 602
BisI GCNGC 4 cut(s) 259, 335, 438, 833
BlsI GCNGC 4 cut(s) 260, 336, 439, 834
Bme1390I CCNGG 2 cut(s) 174, 402
Bme18I GGWCC 3 cut(s) 136, 409, 623
BmeT110I CYCGRG 2 cut(s) 635, 661
BmgT120I GGNCC 4 cut(s) 136, 409, 542, 623
BmiI GGNNCC 6 cut(s) 121, 151, 229, 447, 543, 625
BmrFI CCNGG 2 cut(s) 174, 402
BpiI GAAGAC 1 cut(s) 566
BpmI CTGGAG 2 cut(s) 233, 377
BpuMI CCSGG 2 cut(s) 174, 402
BsaAI YACGTR 2 cut(s) 133, 703
BsaHI GRCGYC 2 cut(s) 164, 645
BsaJI CCNNGG 4 cut(s) 112, 144, 484, 545
BsaWI WCCGGW 4 cut(s) 24, 474, 501, 528
BsaXI ACNNNNNCTCC 8 cut(s) 14, 44, 83, 113, 170, 200, 794, 824
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 145, 159
Bse118I RCCGGY 3 cut(s) 233, 458, 474
Bse1I ACTGG 2 cut(s) 98, 272
BseAI TCCGGA 1 cut(s) 501
BseDI CCNNGG 4 cut(s) 112, 144, 484, 545
BseGI GGATG 2 cut(s) 480, 760
BseLI CCNNNNNNNGG 3 cut(s) 93, 145, 159
BseMII CTCAG 2 cut(s) 286, 359
BseNI ACTGG 2 cut(s) 98, 272
BseRI GAGGAG 1 cut(s) 458
BseXI GCAGC 1 cut(s) 321
BseYI CCCAGC 2 cut(s) 159, 589
Bsh1285I CGRYCG 1 cut(s) 822
BshFI GGCC 3 cut(s) 326, 544, 677
BshTI ACCGGT 1 cut(s) 474
BsiEI CGRYCG 1 cut(s) 822
BsiHKAI GWGCWC 2 cut(s) 32, 662
BsiHKCI CYCGRG 2 cut(s) 635, 661
BsiSI CCGG 9 cut(s) 25, 174, 234, 402, 459, 475, 502, 529, 888
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 3 cut(s) 93, 145, 159
BsmFI GGGAC 1 cut(s) 151
BsnI GGCC 3 cut(s) 326, 544, 677
BsoBI CYCGRG 2 cut(s) 635, 661
Bsp1286I GDGCHC 2 cut(s) 32, 662
Bsp13I TCCGGA 1 cut(s) 501
Bsp143I GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 837
Bsp68I TCGCGA 2 cut(s) 656, 671
BspANI GGCC 3 cut(s) 326, 544, 677
BspCNI CTCAG 2 cut(s) 285, 358
BspEI TCCGGA 1 cut(s) 501
BspLI GGNNCC 6 cut(s) 121, 151, 229, 447, 543, 625
BspPI GGATC 9 cut(s) 104, 114, 127, 270, 440, 453, 539, 557, 674
BspQI GCTCTTC 1 cut(s) 362
BsrFI RCCGGY 3 cut(s) 233, 458, 474
BsrI ACTGG 2 cut(s) 98, 272
BssAI RCCGGY 3 cut(s) 233, 458, 474
BssECI CCNNGG 4 cut(s) 112, 144, 484, 545
BssMI GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 837
BssNI GRCGYC 2 cut(s) 164, 645
Bst4CI ACNGT 3 cut(s) 565, 578, 820
Bst6I CTCTTC 1 cut(s) 362
BstACI GRCGYC 2 cut(s) 164, 645
BstBAI YACGTR 2 cut(s) 133, 703
BstC8I GCNNGC 5 cut(s) 235, 307, 460, 658, 747
BstDEI CTNAG 2 cut(s) 272, 345
BstDSI CCRYGG 4 cut(s) 112, 144, 484, 545
BstF5I GGATG 2 cut(s) 480, 760
BstH2I RGCGCY 1 cut(s) 602
BstHHI GCGC 8 cut(s) 129, 334, 422, 601, 745, 782, 832, 847
BstKTI GATC 9 cut(s) 99, 122, 278, 448, 522, 534, 552, 669, 840
BstMBI GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 837
BstMCI CGRYCG 1 cut(s) 822
BstMWI GCNNNNNNNGC 2 cut(s) 44, 234
BstSCI CCNGG 2 cut(s) 172, 400
BstV1I GCAGC 1 cut(s) 321
BstV2I GAAGAC 1 cut(s) 566
BstX2I RGATCY 4 cut(s) 96, 119, 275, 445
BstYI RGATCY 4 cut(s) 96, 119, 275, 445
BsuRI GGCC 3 cut(s) 326, 544, 677
BtgI CCRYGG 4 cut(s) 112, 144, 484, 545
BtsCI GGATG 2 cut(s) 480, 760
BtsIMutI CAGTG 1 cut(s) 583
BtuMI TCGCGA 2 cut(s) 656, 671
Cac8I GCNNGC 5 cut(s) 235, 307, 460, 658, 747
CfoI GCGC 8 cut(s) 129, 334, 422, 601, 745, 782, 832, 847
Cfr10I RCCGGY 3 cut(s) 233, 458, 474
Cfr13I GGNCC 4 cut(s) 136, 409, 542, 623
CseI GACGC 4 cut(s) 153, 503, 836, 852
Csp6I GTAC 2 cut(s) 299, 700
CspAI ACCGGT 1 cut(s) 474
CviAII CATG 1 cut(s) 724
CviQI GTAC 2 cut(s) 299, 700
DdeI CTNAG 2 cut(s) 272, 345
DpnI GATC 9 cut(s) 98, 121, 277, 447, 521, 533, 551, 668, 839
DpnII GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 837
EaeI YGGCCR 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
EciI GGCGGA 4 cut(s) 132, 220, 455, 764
Ecl136II GAGCTC 2 cut(s) 30, 660
Eco24I GRGCYC 2 cut(s) 32, 662
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 3 cut(s) 136, 409, 623
Eco53kI GAGCTC 2 cut(s) 30, 660
Eco72I CACGTG 1 cut(s) 133
Eco88I CYCGRG 2 cut(s) 635, 661
EcoICRI GAGCTC 2 cut(s) 30, 660
EcoO109I RGGNCCY 1 cut(s) 623
EcoRV GATATC 1 cut(s) 652
EcoT38I GRGCYC 2 cut(s) 32, 662
FaeI CATG 1 cut(s) 727
FaiI YATR 3 cut(s) 725, 793, 882
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FaqI GGGAC 1 cut(s) 151
FatI CATG 1 cut(s) 723
FauI CCCGC 2 cut(s) 314, 463
Fnu4HI GCNGC 4 cut(s) 259, 335, 438, 833
FokI GGATG 2 cut(s) 467, 767
FriOI GRGCYC 2 cut(s) 32, 662
Fsp4HI GCNGC 4 cut(s) 259, 335, 438, 833
GlaI GCGC 8 cut(s) 128, 333, 421, 600, 744, 781, 831, 846
GluI GCNGC 4 cut(s) 259, 335, 438, 833
GsaI CCCAGC 2 cut(s) 163, 593
GsuI CTGGAG 2 cut(s) 233, 377
HaeII RGCGCY 1 cut(s) 602
HaeIII GGCC 3 cut(s) 326, 544, 677
HapII CCGG 9 cut(s) 25, 174, 234, 402, 459, 475, 502, 529, 888
HgaI GACGC 4 cut(s) 153, 503, 836, 852
HhaI GCGC 8 cut(s) 129, 334, 422, 601, 745, 782, 832, 847
Hin1I GRCGYC 2 cut(s) 164, 645
Hin1II CATG 1 cut(s) 727
Hin6I GCGC 8 cut(s) 127, 332, 420, 599, 743, 780, 830, 845
HinP1I GCGC 8 cut(s) 127, 332, 420, 599, 743, 780, 830, 845
HinfI GANTC 8 cut(s) 62, 71, 104, 388, 397, 505, 687, 874
HpaII CCGG 9 cut(s) 25, 174, 234, 402, 459, 475, 502, 529, 888
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 4 cut(s) 112, 136, 526, 826
Hpy188I TCNGA 4 cut(s) 61, 67, 103, 275
Hpy188III TCNNGA 9 cut(s) 394, 502, 517, 523, 535, 556, 637, 655, 670
Hpy8I GTNNAC 4 cut(s) 112, 136, 526, 826
HpyAV CCTTC 4 cut(s) 334, 532, 589, 667
HpyCH4III ACNGT 3 cut(s) 565, 578, 820
HpyCH4IV ACGT 4 cut(s) 132, 297, 645, 702
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 234
HpyF3I CTNAG 2 cut(s) 272, 345
HpySE526I ACGT 4 cut(s) 132, 297, 645, 702
Hsp92I GRCGYC 2 cut(s) 164, 645
Hsp92II CATG 1 cut(s) 727
HspAI GCGC 8 cut(s) 127, 332, 420, 599, 743, 780, 830, 845
Kpn2I TCCGGA 1 cut(s) 501
KroI GCCGGC 2 cut(s) 233, 458
KroNI GCCGGC 2 cut(s) 235, 460
Kzo9I GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 837
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 4 cut(s) 27, 35, 62, 233
Lsp1109I GCAGC 1 cut(s) 321
MaeII ACGT 4 cut(s) 132, 297, 645, 702
MaeIII GTNAC 1 cut(s) 703
MalI GATC 9 cut(s) 98, 121, 277, 447, 521, 533, 551, 668, 839
MboI GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 837
MboII GAAGA 2 cut(s) 379, 571
MflI RGATCY 4 cut(s) 96, 119, 275, 445
MhlI GDGCHC 2 cut(s) 32, 662
MlsI TGGCCA 1 cut(s) 326
MluCI AATT 2 cut(s) 571, 610
MluI ACGCGT 1 cut(s) 495
MluNI TGGCCA 1 cut(s) 326
MlyI GAGTC 2 cut(s) 113, 681
MmeI TCCRAC 1 cut(s) 32
Mox20I TGGCCA 1 cut(s) 326
MreI CGCCGGCG 1 cut(s) 233
MroI TCCGGA 1 cut(s) 501
MroNI GCCGGC 2 cut(s) 233, 458
MroXI GAANNNNTTC 1 cut(s) 371
MscI TGGCCA 1 cut(s) 326
Msp20I TGGCCA 1 cut(s) 326
MspA1I CMGCKG 1 cut(s) 511
MspI CCGG 9 cut(s) 25, 174, 234, 402, 459, 475, 502, 529, 888
MspR9I CCNGG 2 cut(s) 174, 402
MwoI GCNNNNNNNGC 2 cut(s) 44, 234
NaeI GCCGGC 2 cut(s) 235, 460
NciI CCSGG 2 cut(s) 174, 402
NdeII GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 837
NgoMIV GCCGGC 2 cut(s) 233, 458
NlaIII CATG 1 cut(s) 727
NlaIV GGNNCC 6 cut(s) 121, 151, 229, 447, 543, 625
NmuCI GTSAC 1 cut(s) 703
NruI TCGCGA 2 cut(s) 656, 671
PaeR7I CTCGAG 1 cut(s) 635
PciSI GCTCTTC 1 cut(s) 362
PcsI WCGNNNNNNNCGW 6 cut(s) 309, 642, 668, 708, 713, 900
PdiI GCCGGC 2 cut(s) 235, 460
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 6 cut(s) 62, 71, 388, 397, 505, 874
PflMI CCANNNNNTGG 1 cut(s) 159
PinAI ACCGGT 1 cut(s) 474
PkrI GCNGC 4 cut(s) 260, 336, 439, 834
PleI GAGTC 2 cut(s) 112, 681
PmaCI CACGTG 1 cut(s) 133
PmlI CACGTG 1 cut(s) 133
PpsI GAGTC 2 cut(s) 112, 681
Ppu21I YACGTR 2 cut(s) 133, 703
PpuMI RGGWCCY 1 cut(s) 623
Psp124BI GAGCTC 2 cut(s) 32, 662
Psp5II RGGWCCY 1 cut(s) 623
PspCI CACGTG 1 cut(s) 133
PspFI CCCAGC 2 cut(s) 159, 589
PspN4I GGNNCC 6 cut(s) 121, 151, 229, 447, 543, 625
PspPI GGNCC 4 cut(s) 136, 409, 542, 623
PspPPI RGGWCCY 1 cut(s) 623
PsuI RGATCY 4 cut(s) 96, 119, 275, 445
RruI TCGCGA 2 cut(s) 656, 671
RsaI GTAC 2 cut(s) 300, 701
RsaNI GTAC 2 cut(s) 299, 700
SacI GAGCTC 2 cut(s) 32, 662
SapI GCTCTTC 1 cut(s) 362
SatI GCNGC 4 cut(s) 259, 335, 438, 833
Sau3AI GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 837
Sau96I GGNCC 4 cut(s) 136, 409, 542, 623
SchI GAGTC 2 cut(s) 113, 681
ScrFI CCNGG 2 cut(s) 174, 402
SduI GDGCHC 2 cut(s) 32, 662
Sfr274I CTCGAG 1 cut(s) 635
SgrAI CRCCGGYG 1 cut(s) 233
SinI GGWCC 3 cut(s) 136, 409, 623
SlaI CTCGAG 1 cut(s) 635
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
Sse9I AATT 2 cut(s) 571, 610
SstI GAGCTC 2 cut(s) 32, 662
StyD4I CCNGG 2 cut(s) 172, 400
TaaI ACNGT 3 cut(s) 565, 578, 820
TaiI ACGT 4 cut(s) 135, 300, 648, 705
TaqI TCGA 6 cut(s) 212, 291, 362, 636, 648, 866
TasI AATT 2 cut(s) 571, 610
TauI GCSGC 3 cut(s) 261, 440, 835
TfiI GAWTC 6 cut(s) 62, 71, 388, 397, 505, 874
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 1 cut(s) 334
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 3 cut(s) 364, 380, 469
TspGWI ACGGA 2 cut(s) 22, 97
TspRI CASTG 1 cut(s) 583
Van91I CCANNNNNTGG 1 cut(s) 159
VpaK11BI GGWCC 3 cut(s) 136, 409, 623
XhoI CTCGAG 1 cut(s) 635
XmnI GAANNNNTTC 1 cut(s) 371
ZraI GACGTC 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.