pycom08g04180

Protein ENHANCED DISEASE RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010107
Physical Location & Seq
Forward (+)
3213360 .. 3214277
918 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04180.1

Sequence Viewer

Length: 918 bp
ATGTGTCCAACAAAGCAAAAGCACCGGAGCTCCGTCGCCGACCACGCCGGAAAATGCTCCAAATCTGAGGATTCTACAACCTCCACCGCCGACTGGATCTCCGAGTCCGTCCACGGCGGATCGATGCGCCACGTGGACCTCACCACCGGAACCAACGGCTGGGCGTCCCCTCCCGGCGACCTATTCTCCCTCCGCACCAAGAAATACTTGTCGAATAAACAAAAAGGCCCCGCCGGCGACTACCTACTCCAGCCGTGCGGCACCGACTGGCTCAGATCCAGCACGAAACTCGATAACGTACTCGCCCGCCCCGATAATCGCGTGGCCAATGCGCTGCGGAAGGCTCAGTCCCAAGGGAAGTCGATGAAGAGCTTCATTTTTGCCGTGAATCTCCAGATTCCCGGCAAGGACCAGCACAGCGCGGTTTTCTACTTCGCGGCGGAGGATCCAATCCCCGCCGGCTCTCTCCTCTACCGGTTCATCCACGGCGACGACGCGTTCCGGAATCAGCGGTTCAAGATCGTGAACCGGATCGTGAAGGGGCCGTGGATCGTCGTGAAGACGGTGGGGAATTACAGTGCGTGTTTGCTGGGGAAGGCGCTGACGTGTAATTACCACAGAGGACCCAACTACCTGGAGATTGACGTCGATATCGCGAGCTCGGGGATCGCAAAGGCCATTTTGCGACTCGCATTGAGGTACGTGACGAGCGTGACGATAGACATGGGGTTTGTTGTGGAGGCGCAGGCGGAGGATGAGCTGCCTGAGAAGTTAGTCGGCGCGGTTAGGGTATGCCAGATGGAGATGTCGTCGGCGACGGTCGTGGACGCGCTGCAGACGACGATCGTAGCGCGTGGATTGAGTTTCGCGTCGAAGGTGAATCATCATAAGTCCGGCGACGACGACGACGACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

306

Amino Acids

33.32

Weight (kDa)

8.64

Isoelectric Point (pI)

32.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 54 - 268 3.9e-77 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 159
AccII CGCG 9 cut(s) 321, 422, 437, 497, 656, 782, 830, 853, 869
AccIII TCCGGA 1 cut(s) 501
AclWI GGATC 8 cut(s) 104, 127, 270, 440, 453, 539, 557, 674
AcoI YGGCCR 1 cut(s) 324
AcvI CACGTG 1 cut(s) 133
AcyI GRCGYC 2 cut(s) 164, 645
AfaI GTAC 2 cut(s) 300, 701
AfiI CCNNNNNNNGG 2 cut(s) 93, 159
AflIII ACRYGT 2 cut(s) 495, 605
AgeI ACCGGT 1 cut(s) 474
AgsI TTSAA 1 cut(s) 517
AjiI CACGTC 1 cut(s) 606
AjnI CCWGG 1 cut(s) 633
AluBI AGCT 4 cut(s) 30, 372, 660, 760
AluI AGCT 4 cut(s) 30, 372, 660, 760
Alw21I GWGCWC 2 cut(s) 32, 662
AlwI GGATC 8 cut(s) 104, 127, 270, 440, 453, 539, 557, 674
Ama87I CYCGRG 1 cut(s) 661
Aor13HI TCCGGA 1 cut(s) 501
AoxI GGCC 4 cut(s) 226, 324, 542, 675
ApeKI GCWGC 3 cut(s) 334, 760, 832
ArsI GACNNNNNNTTYG 2 cut(s) 713, 745
AsiGI ACCGGT 1 cut(s) 474
Asp700I GAANNNNTTC 1 cut(s) 371
AspLEI GCGC 8 cut(s) 129, 334, 422, 601, 745, 782, 832, 853
AspS9I GGNCC 5 cut(s) 136, 227, 409, 542, 623
AsuC2I CCSGG 2 cut(s) 174, 402
AsuHPI GGTGA 2 cut(s) 133, 889
AvaI CYCGRG 1 cut(s) 661
AvaII GGWCC 3 cut(s) 136, 409, 623
BalI TGGCCA 1 cut(s) 326
BamHI GGATCC 1 cut(s) 445
BanI GGYRCC 1 cut(s) 260
BanII GRGCYC 2 cut(s) 32, 662
BbrPI CACGTG 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 566
Bbv12I GWGCWC 2 cut(s) 32, 662
BbvI GCAGC 3 cut(s) 321, 747, 819
BccI CCATC 1 cut(s) 793
BceAI ACGGC 6 cut(s) 130, 172, 238, 368, 502, 529
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BciT130I CCWGG 1 cut(s) 635
BcnI CCSGG 2 cut(s) 174, 402
BfmI CTRYAG 1 cut(s) 833
BfoI RGCGCY 1 cut(s) 602
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 5 cut(s) 259, 335, 438, 761, 833
BlsI GCNGC 5 cut(s) 260, 336, 439, 762, 834
Bme1390I CCNGG 3 cut(s) 174, 402, 635
Bme18I GGWCC 3 cut(s) 136, 409, 623
BmeT110I CYCGRG 1 cut(s) 661
BmgBI CACGTC 1 cut(s) 606
BmgT120I GGNCC 5 cut(s) 136, 227, 409, 542, 623
BmiI GGNNCC 6 cut(s) 151, 229, 262, 447, 543, 625
BmrFI CCNGG 3 cut(s) 174, 402, 635
BmsI GCATC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 566
BpmI CTGGAG 3 cut(s) 233, 377, 656
BpuMI CCSGG 2 cut(s) 174, 402
Bsa29I ATCGAT 1 cut(s) 122
BsaAI YACGTR 2 cut(s) 133, 703
BsaHI GRCGYC 2 cut(s) 164, 645
BsaJI CCNNGG 4 cut(s) 112, 352, 484, 545
BsaWI WCCGGW 5 cut(s) 24, 146, 474, 501, 528
BsaXI ACNNNNNCTCC 8 cut(s) 14, 44, 83, 113, 170, 200, 794, 824
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 159
Bse118I RCCGGY 3 cut(s) 233, 458, 474
Bse1I ACTGG 2 cut(s) 98, 272
BseAI TCCGGA 1 cut(s) 501
BseBI CCWGG 1 cut(s) 635
BseCI ATCGAT 1 cut(s) 122
BseDI CCNNGG 4 cut(s) 112, 352, 484, 545
BseGI GGATG 2 cut(s) 480, 760
BseLI CCNNNNNNNGG 2 cut(s) 93, 159
BseMII CTCAG 4 cut(s) 57, 286, 359, 756
BseNI ACTGG 2 cut(s) 98, 272
BseRI GAGGAG 1 cut(s) 458
BseXI GCAGC 3 cut(s) 321, 747, 819
BseYI CCCAGC 2 cut(s) 159, 589
Bsh1236I CGCG 9 cut(s) 321, 422, 437, 497, 656, 782, 830, 853, 869
Bsh1285I CGRYCG 2 cut(s) 822, 846
BshFI GGCC 4 cut(s) 228, 326, 544, 677
BshNI GGYRCC 1 cut(s) 260
BshTI ACCGGT 1 cut(s) 474
BshVI ATCGAT 1 cut(s) 122
BsiEI CGRYCG 2 cut(s) 822, 846
BsiHKAI GWGCWC 2 cut(s) 32, 662
BsiHKCI CYCGRG 1 cut(s) 661
BslFI GGGAC 2 cut(s) 151, 334
BslI CCNNNNNNNGG 2 cut(s) 93, 159
BsmFI GGGAC 2 cut(s) 151, 334
BsnI GGCC 4 cut(s) 228, 326, 544, 677
BsoBI CYCGRG 1 cut(s) 661
Bsp1286I GDGCHC 2 cut(s) 32, 662
Bsp13I TCCGGA 1 cut(s) 501
Bsp143I GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 843
Bsp68I TCGCGA 1 cut(s) 656
BspANI GGCC 4 cut(s) 228, 326, 544, 677
BspCNI CTCAG 4 cut(s) 58, 285, 358, 757
BspDI ATCGAT 1 cut(s) 122
BspEI TCCGGA 1 cut(s) 501
BspFNI CGCG 9 cut(s) 321, 422, 437, 497, 656, 782, 830, 853, 869
BspLI GGNNCC 6 cut(s) 151, 229, 262, 447, 543, 625
BspMAI CTGCAG 1 cut(s) 837
BspPI GGATC 8 cut(s) 104, 127, 270, 440, 453, 539, 557, 674
BspQI GCTCTTC 1 cut(s) 362
BspT107I GGYRCC 1 cut(s) 260
BsrFI RCCGGY 3 cut(s) 233, 458, 474
BsrI ACTGG 2 cut(s) 98, 272
BssAI RCCGGY 3 cut(s) 233, 458, 474
BssECI CCNNGG 4 cut(s) 112, 352, 484, 545
BssMI GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 843
BssNI GRCGYC 2 cut(s) 164, 645
BssT1I CCWWGG 1 cut(s) 352
Bst2UI CCWGG 1 cut(s) 635
Bst4CI ACNGT 3 cut(s) 565, 578, 820
Bst6I CTCTTC 1 cut(s) 362
BstACI GRCGYC 2 cut(s) 164, 645
BstBAI YACGTR 2 cut(s) 133, 703
BstC8I GCNNGC 5 cut(s) 235, 307, 460, 658, 747
BstDEI CTNAG 4 cut(s) 66, 272, 345, 765
BstDSI CCRYGG 3 cut(s) 112, 484, 545
BstF5I GGATG 2 cut(s) 480, 760
BstFNI CGCG 9 cut(s) 321, 422, 437, 497, 656, 782, 830, 853, 869
BstH2I RGCGCY 1 cut(s) 602
BstHHI GCGC 8 cut(s) 129, 334, 422, 601, 745, 782, 832, 853
BstKTI GATC 9 cut(s) 99, 122, 278, 448, 522, 534, 552, 669, 846
BstMBI GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 843
BstMCI CGRYCG 2 cut(s) 822, 846
BstMWI GCNNNNNNNGC 2 cut(s) 44, 234
BstNI CCWGG 1 cut(s) 635
BstSCI CCNGG 3 cut(s) 172, 400, 633
BstSFI CTRYAG 1 cut(s) 833
BstUI CGCG 9 cut(s) 321, 422, 437, 497, 656, 782, 830, 853, 869
BstV1I GCAGC 3 cut(s) 321, 747, 819
BstV2I GAAGAC 1 cut(s) 566
BstX2I RGATCY 3 cut(s) 96, 275, 445
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 3 cut(s) 96, 275, 445
Bsu15I ATCGAT 1 cut(s) 122
BsuRI GGCC 4 cut(s) 228, 326, 544, 677
BsuTUI ATCGAT 1 cut(s) 122
BtgI CCRYGG 3 cut(s) 112, 484, 545
BtrI CACGTC 1 cut(s) 606
BtsCI GGATG 2 cut(s) 480, 760
BtsIMutI CAGTG 1 cut(s) 583
BtuMI TCGCGA 1 cut(s) 656
Cac8I GCNNGC 5 cut(s) 235, 307, 460, 658, 747
CfoI GCGC 8 cut(s) 129, 334, 422, 601, 745, 782, 832, 853
Cfr10I RCCGGY 3 cut(s) 233, 458, 474
Cfr13I GGNCC 5 cut(s) 136, 227, 409, 542, 623
ClaI ATCGAT 1 cut(s) 122
CseI GACGC 4 cut(s) 153, 503, 836, 858
Csp6I GTAC 2 cut(s) 299, 700
CspAI ACCGGT 1 cut(s) 474
CviAII CATG 1 cut(s) 724
CviQI GTAC 2 cut(s) 299, 700
DdeI CTNAG 4 cut(s) 66, 272, 345, 765
DpnI GATC 9 cut(s) 98, 121, 277, 447, 521, 533, 551, 668, 845
DpnII GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 843
EaeI YGGCCR 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
EciI GGCGGA 3 cut(s) 132, 455, 764
Ecl136II GAGCTC 2 cut(s) 30, 660
Eco130I CCWWGG 1 cut(s) 352
Eco24I GRGCYC 2 cut(s) 32, 662
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 3 cut(s) 136, 409, 623
Eco53kI GAGCTC 2 cut(s) 30, 660
Eco72I CACGTG 1 cut(s) 133
Eco88I CYCGRG 1 cut(s) 661
EcoICRI GAGCTC 2 cut(s) 30, 660
EcoO109I RGGNCCY 2 cut(s) 227, 623
EcoRII CCWGG 1 cut(s) 633
EcoRV GATATC 1 cut(s) 652
EcoT14I CCWWGG 1 cut(s) 352
EcoT38I GRGCYC 2 cut(s) 32, 662
ErhI CCWWGG 1 cut(s) 352
FaeI CATG 1 cut(s) 727
FaiI YATR 3 cut(s) 725, 793, 888
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FaqI GGGAC 2 cut(s) 151, 334
FatI CATG 1 cut(s) 723
FauI CCCGC 3 cut(s) 238, 314, 463
Fnu4HI GCNGC 5 cut(s) 259, 335, 438, 761, 833
FokI GGATG 2 cut(s) 467, 767
FriOI GRGCYC 2 cut(s) 32, 662
Fsp4HI GCNGC 5 cut(s) 259, 335, 438, 761, 833
GlaI GCGC 8 cut(s) 128, 333, 421, 600, 744, 781, 831, 852
GluI GCNGC 5 cut(s) 259, 335, 438, 761, 833
GsaI CCCAGC 2 cut(s) 163, 593
GsuI CTGGAG 3 cut(s) 233, 377, 656
HaeII RGCGCY 1 cut(s) 602
HaeIII GGCC 4 cut(s) 228, 326, 544, 677
HgaI GACGC 4 cut(s) 153, 503, 836, 858
HhaI GCGC 8 cut(s) 129, 334, 422, 601, 745, 782, 832, 853
Hin1I GRCGYC 2 cut(s) 164, 645
Hin1II CATG 1 cut(s) 727
Hin6I GCGC 8 cut(s) 127, 332, 420, 599, 743, 780, 830, 851
HinP1I GCGC 8 cut(s) 127, 332, 420, 599, 743, 780, 830, 851
HinfI GANTC 7 cut(s) 71, 104, 388, 397, 505, 687, 880
HphI GGTGA 2 cut(s) 133, 889
Hpy166II GTNNAC 4 cut(s) 112, 136, 526, 826
Hpy188I TCNGA 3 cut(s) 67, 103, 275
Hpy188III TCNNGA 7 cut(s) 394, 502, 517, 523, 535, 556, 655
Hpy8I GTNNAC 4 cut(s) 112, 136, 526, 826
HpyAV CCTTC 4 cut(s) 334, 532, 589, 868
HpyCH4III ACNGT 3 cut(s) 565, 578, 820
HpyCH4IV ACGT 5 cut(s) 132, 297, 605, 645, 702
HpyCH4V TGCA 1 cut(s) 835
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 234
HpyF3I CTNAG 4 cut(s) 66, 272, 345, 765
HpySE526I ACGT 5 cut(s) 132, 297, 605, 645, 702
Hsp92I GRCGYC 2 cut(s) 164, 645
Hsp92II CATG 1 cut(s) 727
HspAI GCGC 8 cut(s) 127, 332, 420, 599, 743, 780, 830, 851
Kpn2I TCCGGA 1 cut(s) 501
KroI GCCGGC 2 cut(s) 233, 458
KroNI GCCGGC 2 cut(s) 235, 460
Kzo9I GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 843
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 3 cut(s) 27, 35, 62
Lsp1109I GCAGC 3 cut(s) 321, 747, 819
LweI GCATC 1 cut(s) 114
MaeII ACGT 5 cut(s) 132, 297, 605, 645, 702
MaeIII GTNAC 2 cut(s) 703, 712
MalI GATC 9 cut(s) 98, 121, 277, 447, 521, 533, 551, 668, 845
MboI GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 843
MboII GAAGA 2 cut(s) 379, 571
MflI RGATCY 3 cut(s) 96, 275, 445
MhlI GDGCHC 2 cut(s) 32, 662
MlsI TGGCCA 1 cut(s) 326
MluCI AATT 2 cut(s) 571, 610
MluI ACGCGT 1 cut(s) 495
MluNI TGGCCA 1 cut(s) 326
MlyI GAGTC 2 cut(s) 113, 681
MmeI TCCRAC 1 cut(s) 32
Mox20I TGGCCA 1 cut(s) 326
MreI CGCCGGCG 1 cut(s) 233
MroI TCCGGA 1 cut(s) 501
MroNI GCCGGC 2 cut(s) 233, 458
MroXI GAANNNNTTC 1 cut(s) 371
MscI TGGCCA 1 cut(s) 326
Msp20I TGGCCA 1 cut(s) 326
MspA1I CMGCKG 1 cut(s) 511
MspR9I CCNGG 3 cut(s) 174, 402, 635
MvaI CCWGG 1 cut(s) 635
MvnI CGCG 9 cut(s) 321, 422, 437, 497, 656, 782, 830, 853, 869
MwoI GCNNNNNNNGC 2 cut(s) 44, 234
NaeI GCCGGC 2 cut(s) 235, 460
NciI CCSGG 2 cut(s) 174, 402
NdeII GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 843
NgoMIV GCCGGC 2 cut(s) 233, 458
NlaIII CATG 1 cut(s) 727
NlaIV GGNNCC 6 cut(s) 151, 229, 262, 447, 543, 625
NmuCI GTSAC 2 cut(s) 703, 712
NruI TCGCGA 1 cut(s) 656
PciSI GCTCTTC 1 cut(s) 362
PcsI WCGNNNNNNNCGW 5 cut(s) 309, 708, 713, 906, 909
PdiI GCCGGC 2 cut(s) 235, 460
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 5 cut(s) 71, 388, 397, 505, 880
PflMI CCANNNNNTGG 1 cut(s) 159
PinAI ACCGGT 1 cut(s) 474
PkrI GCNGC 5 cut(s) 260, 336, 439, 762, 834
Ple19I CGATCG 1 cut(s) 846
PleI GAGTC 2 cut(s) 112, 681
PmaCI CACGTG 1 cut(s) 133
PmlI CACGTG 1 cut(s) 133
PpsI GAGTC 2 cut(s) 112, 681
Ppu21I YACGTR 2 cut(s) 133, 703
PpuMI RGGWCCY 1 cut(s) 623
Psp124BI GAGCTC 2 cut(s) 32, 662
Psp5II RGGWCCY 1 cut(s) 623
Psp6I CCWGG 1 cut(s) 633
PspCI CACGTG 1 cut(s) 133
PspFI CCCAGC 2 cut(s) 159, 589
PspGI CCWGG 1 cut(s) 633
PspN4I GGNNCC 6 cut(s) 151, 229, 262, 447, 543, 625
PspPI GGNCC 5 cut(s) 136, 227, 409, 542, 623
PspPPI RGGWCCY 1 cut(s) 623
PstI CTGCAG 1 cut(s) 837
PsuI RGATCY 3 cut(s) 96, 275, 445
PvuI CGATCG 1 cut(s) 846
RruI TCGCGA 1 cut(s) 656
RsaI GTAC 2 cut(s) 300, 701
RsaNI GTAC 2 cut(s) 299, 700
SacI GAGCTC 2 cut(s) 32, 662
SapI GCTCTTC 1 cut(s) 362
SatI GCNGC 5 cut(s) 259, 335, 438, 761, 833
Sau3AI GATC 9 cut(s) 96, 119, 275, 445, 519, 531, 549, 666, 843
Sau96I GGNCC 5 cut(s) 136, 227, 409, 542, 623
SchI GAGTC 2 cut(s) 113, 681
ScrFI CCNGG 3 cut(s) 174, 402, 635
SduI GDGCHC 2 cut(s) 32, 662
SfaNI GCATC 1 cut(s) 114
SfcI CTRYAG 1 cut(s) 833
SgrAI CRCCGGYG 1 cut(s) 233
SinI GGWCC 3 cut(s) 136, 409, 623
Sse9I AATT 2 cut(s) 571, 610
SstI GAGCTC 2 cut(s) 32, 662
StyD4I CCNGG 3 cut(s) 172, 400, 633
StyI CCWWGG 1 cut(s) 352
TaaI ACNGT 3 cut(s) 565, 578, 820
TaiI ACGT 5 cut(s) 135, 300, 608, 648, 705
TaqI TCGA 6 cut(s) 122, 212, 291, 362, 648, 872
TasI AATT 2 cut(s) 571, 610
TauI GCSGC 2 cut(s) 261, 440
TfiI GAWTC 5 cut(s) 71, 388, 397, 505, 880
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 2 cut(s) 703, 712
TseI GCWGC 3 cut(s) 334, 760, 832
Tsp45I GTSAC 2 cut(s) 703, 712
TspDTI ATGAA 3 cut(s) 364, 380, 469
TspGWI ACGGA 2 cut(s) 22, 97
TspRI CASTG 1 cut(s) 583
Van91I CCANNNNNTGG 1 cut(s) 159
VpaK11BI GGWCC 3 cut(s) 136, 409, 623
XmnI GAANNNNTTC 1 cut(s) 371
ZraI GACGTC 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.