Rh2DG135400

Protein ENHANCED DISEASE RESISTANCE 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0010107
Physical Location & Seq
Forward (+)
11390589 .. 11390903
315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG135400.1

Sequence Viewer

Length: 315 bp
ATGTCAGGCAACAGCCCCGTTGTCAATTGCGAGCGACAGCGCGAGCCAGGTGGTGTGTCGTCGAAGCGGAAACTGGAAGAGTATGGCAGCCTCTTGATGTTTTCGACCGATAAGGACGATGACGTCGTTTCTGACCTACTCCAAAAATTCTACTTCGCCGCAGAGGATCCTATTCCATCCGGATCACTCCTCCACTGGTTCATCAACGGCGACTACTCATTCCAAAAACACCGGTTCAAGATCGTGAATCGGATCATGAAAGGGTCGTGGATCATTGAGAAAACGGTGGGCAATTACAACGCGTGTTTACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

104

Amino Acids

11.87

Weight (kDa)

6.72

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 50 - 103 3.7e-09 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AatII GACGTC 1 cut(s) 126
AccII CGCG 2 cut(s) 42, 302
AccIII TCCGGA 1 cut(s) 179
AciI CCGC 2 cut(s) 67, 159
AclWI GGATC 5 cut(s) 161, 174, 190, 260, 278
AcsI RAATTY 1 cut(s) 146
AcyI GRCGYC 1 cut(s) 123
AflIII ACRYGT 1 cut(s) 300
AgeI ACCGGT 1 cut(s) 231
AgsI TTSAA 1 cut(s) 238
AjnI CCWGG 1 cut(s) 46
AlwI GGATC 5 cut(s) 161, 174, 190, 260, 278
Aor13HI TCCGGA 1 cut(s) 179
ApeKI GCWGC 1 cut(s) 87
ApoI RAATTY 1 cut(s) 146
AsiGI ACCGGT 1 cut(s) 231
AspLEI GCGC 1 cut(s) 42
BamHI GGATCC 1 cut(s) 166
BbvI GCAGC 1 cut(s) 99
BccI CCATC 1 cut(s) 184
BceAI ACGGC 1 cut(s) 223
BciT130I CCWGG 1 cut(s) 48
BfaI CTAG 1 cut(s) 313
BisI GCNGC 2 cut(s) 88, 159
BlsI GCNGC 2 cut(s) 89, 160
Bme1390I CCNGG 1 cut(s) 48
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 48
BsaHI GRCGYC 1 cut(s) 123
BsaWI WCCGGW 2 cut(s) 179, 231
Bse118I RCCGGY 1 cut(s) 231
Bse1I ACTGG 2 cut(s) 78, 200
BseAI TCCGGA 1 cut(s) 179
BseBI CCWGG 1 cut(s) 48
BseGI GGATG 1 cut(s) 176
BseNI ACTGG 2 cut(s) 78, 200
BseRI GAGGAG 1 cut(s) 179
BseXI GCAGC 1 cut(s) 99
Bsh1236I CGCG 2 cut(s) 42, 302
Bsh1285I CGRYCG 1 cut(s) 108
BshTI ACCGGT 1 cut(s) 231
BsiEI CGRYCG 1 cut(s) 108
BsiSI CCGG 2 cut(s) 180, 232
Bsp13I TCCGGA 1 cut(s) 179
Bsp143I GATC 5 cut(s) 166, 182, 240, 252, 270
BspACI CCGC 2 cut(s) 67, 159
BspEI TCCGGA 1 cut(s) 179
BspFNI CGCG 2 cut(s) 42, 302
BspHI TCATGA 1 cut(s) 255
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 5 cut(s) 161, 174, 190, 260, 278
BsrFI RCCGGY 1 cut(s) 231
BsrI ACTGG 2 cut(s) 78, 200
BssAI RCCGGY 1 cut(s) 231
BssMI GATC 5 cut(s) 166, 182, 240, 252, 270
BssNI GRCGYC 1 cut(s) 123
Bst2UI CCWGG 1 cut(s) 48
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 72
BstACI GRCGYC 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 32, 44
BstF5I GGATG 1 cut(s) 176
BstFNI CGCG 2 cut(s) 42, 302
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 5 cut(s) 169, 185, 243, 255, 273
BstMBI GATC 5 cut(s) 166, 182, 240, 252, 270
BstMCI CGRYCG 1 cut(s) 108
BstNI CCWGG 1 cut(s) 48
BstSCI CCNGG 1 cut(s) 46
BstUI CGCG 2 cut(s) 42, 302
BstV1I GCAGC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BtsCI GGATG 1 cut(s) 176
BtsIMutI CAGTG 1 cut(s) 193
Cac8I GCNNGC 2 cut(s) 32, 44
CciI TCATGA 1 cut(s) 255
CfoI GCGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 231
CspAI ACCGGT 1 cut(s) 231
CviAII CATG 1 cut(s) 256
CviJI RGCY 3 cut(s) 15, 46, 90
CviKI_1 RGCY 3 cut(s) 15, 46, 90
DpnI GATC 5 cut(s) 168, 184, 242, 254, 272
DpnII GATC 5 cut(s) 166, 182, 240, 252, 270
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 46
FaeI CATG 1 cut(s) 259
FaiI YATR 2 cut(s) 84, 257
FatI CATG 1 cut(s) 255
Fnu4HI GCNGC 2 cut(s) 88, 159
FokI GGATG 1 cut(s) 163
Fsp4HI GCNGC 2 cut(s) 88, 159
FspBI CTAG 1 cut(s) 313
GlaI GCGC 1 cut(s) 41
GluI GCNGC 2 cut(s) 88, 159
HapII CCGG 2 cut(s) 180, 232
HhaI GCGC 1 cut(s) 42
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 1 cut(s) 259
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HinfI GANTC 1 cut(s) 247
HpaII CCGG 2 cut(s) 180, 232
Hpy166II GTNNAC 1 cut(s) 308
Hpy188I TCNGA 2 cut(s) 133, 252
Hpy188III TCNNGA 5 cut(s) 94, 180, 238, 244, 256
Hpy8I GTNNAC 1 cut(s) 308
Hpy99I CGWCG 2 cut(s) 64, 128
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 1 cut(s) 123
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 1 cut(s) 259
HspAI GCGC 1 cut(s) 40
Kpn2I TCCGGA 1 cut(s) 179
Kzo9I GATC 5 cut(s) 166, 182, 240, 252, 270
LpnPI CCDG 6 cut(s) 33, 59, 60, 181, 193, 245
Lsp1109I GCAGC 1 cut(s) 99
MaeI CTAG 1 cut(s) 313
MaeII ACGT 1 cut(s) 123
MalI GATC 5 cut(s) 168, 184, 242, 254, 272
MboI GATC 5 cut(s) 166, 182, 240, 252, 270
MboII GAAGA 1 cut(s) 89
MfeI CAATTG 1 cut(s) 25
MflI RGATCY 1 cut(s) 166
MluCI AATT 3 cut(s) 25, 146, 292
MluI ACGCGT 1 cut(s) 300
MnlI CCTC 3 cut(s) 101, 157, 200
MroI TCCGGA 1 cut(s) 179
MspI CCGG 2 cut(s) 180, 232
MspR9I CCNGG 1 cut(s) 48
MunI CAATTG 1 cut(s) 25
MvaI CCWGG 1 cut(s) 48
MvnI CGCG 2 cut(s) 42, 302
NdeII GATC 5 cut(s) 166, 182, 240, 252, 270
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 1 cut(s) 168
PagI TCATGA 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 1 cut(s) 247
PinAI ACCGGT 1 cut(s) 231
PkrI GCNGC 2 cut(s) 89, 160
Psp6I CCWGG 1 cut(s) 46
PspGI CCWGG 1 cut(s) 46
PspN4I GGNNCC 1 cut(s) 168
PsuI RGATCY 1 cut(s) 166
SatI GCNGC 2 cut(s) 88, 159
Sau3AI GATC 5 cut(s) 166, 182, 240, 252, 270
ScrFI CCNGG 1 cut(s) 48
SetI ASST 3 cut(s) 52, 126, 138
Sse9I AATT 3 cut(s) 25, 146, 292
SsiI CCGC 2 cut(s) 67, 159
SspMI CTAG 1 cut(s) 313
StyD4I CCNGG 1 cut(s) 46
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 62, 104
TaqII GACCGA 1 cut(s) 122
TasI AATT 3 cut(s) 25, 146, 292
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 1 cut(s) 247
TscAI CASTG 1 cut(s) 200
TseI GCWGC 1 cut(s) 87
TspDTI ATGAA 2 cut(s) 190, 272
TspRI CASTG 1 cut(s) 200
XapI RAATTY 1 cut(s) 146
XspI CTAG 1 cut(s) 313
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.