AT5G16300

Conserved oligomeric Golgi complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005295
Physical Location & Seq
Forward (+)
5337895 .. 5342432
4538 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16300.2

Sequence Viewer

Length: 3207 bp
ATGAGAATGTCATCAGCTTCCGCCGGGGAATACCGGCCTTCCGCCGTAAGTCTCAGCAGTAACGGAGGTGGTCAACGAGATGCTGAGTCTCTTTTTCGCACGAAACCAATGTCGGAGATTCGAATTGTGGAGTCGGCGACTAGGAAGAACATCGAGGATAAGAAAGAGGAGCTTCGGCAACTCGTTGGGACTAGGTACCGTGATCTAATCGACTCAGCTGACTCGATTGTTCACATGAAGTCGCTCTGTGAGTCGATCTCAGCCAATATCTCTTCGATCCACGGTAACATTCGATCGCTTTCTTCTTCTTCTGTAGCGGAAACTCCTAAGCTTGCTAGCCTTAACCCAGTACGAGTCAATGTCTATGGGATCGCTTGTCGTGTTAAGTATCTGGTGGATACGCCTGAGAATATATGGGGATGCCTTGACGAGTCTATGTTTCTAGAGGCTGCGGGGAGGTACATGCGTGCTCAGCATGTGCAGCAGAGACTGATCAAGTTGGAGGGATGTGGTGGTGGTGTAGCAGAGGTGGATCAGAGTAAGCTGTTGGCGAATTTTCCACTATTGGAGCACCAATGGCAGATAGTTGAGAGTTTTAAGGCTCAGATTTCGCAGAGGAGCCATGAGCGGCTTCTAGATCCGGGTTTAGGACTGGGAGCTTATGTGGATGCCTTAACTGCTGTCGCGGTGGTTGATGAGCTTGATCCAGAGCAAGTGCTTGAGTTGTTTCTAGACTCGAGGAAGACTTGGATTCTGCAAAAGTTGAATGCTTGTACTGGTGAGGATGCGGGTGAGGTTGTTTTGGTGTTTTGTGATGTGTTAAGTGTGATTCAGGTTACAGTGGGACAAGTTGGGGAACTCTTCTTGCAAGCCTTGACTGACATGCCTCTATTTTATAAAACAATCTTGAGTACCCCTCCAGCTTCTCAACTGTTTGGTGGAATTCCGAATCCAGAGGAAGAGGTTGAGTTGTGGAAATCCTTCAGAGATAAACTGGAATCCGTTATGCTCATCCTTGATAAAAATGACGTTTCTAAGTCTTGTCTGACTTGGTTGAGGGAATGCGGTGGACAGATTGTTGGTAAGGTTAGTGGGAAGCATTTGATTGAAGCTATAGTTACTGGCGCGGAGCTTGGATCAGCCGAAAAGTTGATAAGGGAGACCATGGATAGCAAAGATGTCTTGAGGGGTAGTTTGGATTGGCTTAAAAGTGTTTTCGGGTCTGAGGTAGAGTTGCCTTGGAATAGAATCCGAGAGCTTGTTCTGGGGGATGATTTGAACCTATGGGATGAAATATTTGAGAAGGCCTTTGTGGAAAGGATGAAATCTATCATTGACTCCAAATTTGAGAATTTGACTAAAGCTGTCAATGTAGCAGATTCGGTACATGCTTATAGTGAGATTACTGGTGAGAAAATCAACTTCCAAGCATACTTAAACAGACCTTCTACAGGTGGTGGTGTCTGGTTCATTGAGCCTAATTCAAAGAAAGTTGGTCTTATTTCCGGAAATAAATCATCACCTGAAGAAAGCGACTTCCAAAGTTGTCTAACTGCGTACTTTGGTCCTGAAGTTAGCCAAATGAGAGATGCAGTTGATAGGCGCTGCCATAGTGTTTTGGAAGACCTCCTAAGCTTCTTCGAGTCAGAAAAGGCAGGTCCAAGGTTAAAGGATCTTGCACCATACGTGCAGAATAAGTGTTATGACAGTGTCTCAGCACTTCTTGCAGATGTAGATAAAGAACTTGAATTTCTATGTGCTGCCGTGAAGAAAGAGAACAAGGACAGTGAAGCAATTCCACCTGCTATAATAATTGAGAAATCACTTTTTATGGGACGCCTTTTGTTTGCATTGCTGAACCACTCAAAACATGTTCCCTTAATTCTCGGTTCTCCAAGATTGTGGTGTAGAGAAACTATGACTGCAGTTTCTGATAAGTTGTCATCCTTGTTGAGGCAGCCAAGATTTAGCTCGAATACTCCAGCCACTGCTGACAGCCCTGGAAAGCAATTGCATACCGATCTTAGAAAGCAGACTTCATTAGCTGTTGCTGCACTACTGGGAGCGGAAGAGAAAACAAGTCCAAAATTTGAAGAGCTTAATAGAACTATGCGAGATCTGTGCATCAAGGCTCATACATTGTGGATAAAGTGGTTATCTGATGAGCTTTCAGCTATTCTCTTGCGTGATCTTAGGAGTGATGATGGATTGTCTGCTACAACTCCTTTAAGGGGATGGGAAGAGACAATAGTGAAGCAAGAGCAGGACGAGAGTCAATCTGAGTTGAAGATATCACTTCCATCGTTGCCTTCACTCTATATGATTTCATTTCTCTGTCGGGCATCAGAAGAAATTCATCGAATTGGAGGTCACGTACTGGATAGGTCTATTTTGCAAAAATTTGCTTCAAGCCTGTTGGAGAAGATCACCATTATTTATGAGGATTTCTTATCTGCCAGGGAGGCCAGCGAACCCCAAATTTCAGAGAAAGGAGTTTTACAAATTTTGCTGGATTTGAGATTTGCAGCTGACGTTCTTTCTGGGGGTGATACTAGCACCAATGTGGAGACACCAAAGAGCACAATAAATAGATCGGCTTACAGGCGAAGGCAGGATCAACAGAAAACAAAGTTAGTCAATAGAGGTCGCATTGATGGAGTGACCTCCCAACTAACCCAAAAGTTGGATCCCATAGACTGGCTTACGTACGAGCCTTATCTTTGGGAGAACGAAAAGCAGTCGTACCTACGCCATGCTGTACTCTTTGGTTTCTTTGTGCAACTAAACCGAATGTACACAGATACTGCGCAGAAACTATCAATTAATATAGAATCAAATATCATGCCATGTTCCACAGTTCCCCGCTTCAAATACCTTCCCATAAGCGCTCCAGCTCTGTCATCTAGAAGTACAAATAAGGTCTCTATTCCAGTTACATCAAATGATGCTTCGGCAAGAAACTCATGGAAGGCATTTACAAACGGCGAGCAATCTCAAACAAGTGATTTAGAAGAAAATTCCAATTTCGGCGTGGCTTTTAAGTCTTTCATGCAGGAAAGCACATTGAAACTGGGATCAATATTAACTGATGGACAAGTGGGAATATTCAAGGACAGATCAGCAGCCGCTATGTCGACATTTGGCGATATACTACCGGCTCAAGCAGCTGGTTTACTCTCTTCTTTCACAAACACCAGGTCCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps51

Protein Analysis

1068

Amino Acids

118.8

Weight (kDa)

5.51

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS51_Exo84_N PF08700 24 - 102 4.6e-19 Vps51/EXO84/COG1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 897
AarI CACCTGC 1 cut(s) 1810
Acc16I TGCGCA 1 cut(s) 2808
Acc36I ACCTGC 2 cut(s) 1646, 1810
Acc65I GGTACC 1 cut(s) 195
AccB1I GGYRCC 1 cut(s) 195
AccB7I CCANNNNNTGG 2 cut(s) 2683, 2697
AccBSI CCGCTC 2 cut(s) 628, 2066
AccI GTMKAC 1 cut(s) 3134
AccII CGCG 2 cut(s) 686, 1127
AccIII TCCGGA 1 cut(s) 1505
AcuI CTGAAG 3 cut(s) 967, 1545, 1590
AcyI GRCGYC 1 cut(s) 1837
AfeI AGCGCT 1 cut(s) 2887
AfiI CCNNNNNNNGG 6 cut(s) 647, 1451, 1953, 2231, 2683, 2697
AflIII ACRYGT 1 cut(s) 1870
AjnI CCWGG 3 cut(s) 1999, 2456, 3194
AleI CACNNNNGTG 1 cut(s) 2561
Alw21I GWGCWC 3 cut(s) 472, 573, 2582
Alw26I GTCTC 8 cut(s) 56, 93, 481, 1154, 1717, 2237, 2561, 2926
AlwNI CAGNNNCTG 4 cut(s) 490, 1931, 1988, 2804
Ama87I CYCGRG 1 cut(s) 736
Aor13HI TCCGGA 1 cut(s) 1505
Aor51HI AGCGCT 1 cut(s) 2887
AoxI GGCC 3 cut(s) 35, 1305, 2463
ApeKI GCWGC 9 cut(s) 449, 481, 1605, 1760, 1957, 2051, 2525, 3122, 3164
ArsI GACNNNNNNTTYG 7 cut(s) 95, 127, 1328, 1360, 2353, 2385, 3182
AseI ATTAAT 1 cut(s) 2823
Asp700I GAANNNNTTC 3 cut(s) 980, 1794, 2352
Asp718I GGTACC 1 cut(s) 195
AspLEI GCGC 4 cut(s) 1127, 1605, 2809, 2888
AspS9I GGNCC 3 cut(s) 1565, 1658, 3198
AsuC2I CCSGG 2 cut(s) 25, 642
AsuHPI GGTGA 6 cut(s) 791, 803, 1421, 1512, 2419, 2558
AsuII TTCGAA 1 cut(s) 121
AsuNHI GCTAGC 1 cut(s) 335
AvaI CYCGRG 1 cut(s) 736
AvaII GGWCC 3 cut(s) 1565, 1658, 3198
BaeI ACNNNNGTAYC 2 cut(s) 451, 484
BamHI GGATCC 1 cut(s) 2686
BanI GGYRCC 1 cut(s) 195
BbsI GAAGAC 2 cut(s) 749, 1629
Bbv12I GWGCWC 3 cut(s) 472, 573, 2582
BbvI GCAGC 9 cut(s) 436, 493, 1592, 1747, 1969, 2038, 2537, 3134, 3176
BccI CCATC 5 cut(s) 2198, 2229, 2308, 2648, 3083
BceAI ACGGC 3 cut(s) 29, 1748, 2998
BcgI CGANNNNNNTGC 2 cut(s) 2103, 2137
BciT130I CCWGG 3 cut(s) 2001, 2458, 3196
BciVI GTATCC 1 cut(s) 391
BclI TGATCA 1 cut(s) 492
BcnI CCSGG 2 cut(s) 25, 642
BcoDI GTCTC 8 cut(s) 56, 93, 481, 1154, 1717, 2237, 2561, 2926
BfaI CTAG 8 cut(s) 141, 192, 336, 443, 635, 731, 2553, 2904
BfmI CTRYAG 4 cut(s) 312, 1113, 1449, 1923
BfoI RGCGCY 2 cut(s) 1606, 2889
BfuAI ACCTGC 2 cut(s) 1646, 1810
BfuI GTATCC 1 cut(s) 391
BglI GCCNNNNNGGC 1 cut(s) 2462
BglII AGATCT 1 cut(s) 2116
BlpI GCTNAGC 1 cut(s) 471
Bme1390I CCNGG 5 cut(s) 25, 642, 2001, 2458, 3196
Bme18I GGWCC 3 cut(s) 1565, 1658, 3198
BmeT110I CYCGRG 1 cut(s) 736
BmgT120I GGNCC 3 cut(s) 1565, 1658, 3198
BmiI GGNNCC 3 cut(s) 197, 620, 2688
BmrFI CCNGG 5 cut(s) 25, 642, 2001, 2458, 3196
BmrI ACTGGG 4 cut(s) 341, 662, 2069, 3080
BmsI GCATC 8 cut(s) 70, 410, 658, 775, 1579, 2133, 2351, 2935
BmtI GCTAGC 1 cut(s) 339
BmuI ACTGGG 4 cut(s) 341, 662, 2069, 3080
BoxI GACNNNNGTC 1 cut(s) 2271
BpiI GAAGAC 2 cut(s) 749, 1629
BplI GAGNNNNNCTC 4 cut(s) 242, 274, 901, 933
BpmI CTGGAG 3 cut(s) 903, 1965, 2874
Bpu10I CCTNAGC 2 cut(s) 327, 1631
Bpu1102I GCTNAGC 1 cut(s) 471
Bpu14I TTCGAA 1 cut(s) 121
BpuEI CTTGAG 4 cut(s) 740, 928, 1204, 3144
BpuMI CCSGG 2 cut(s) 25, 642
BsaAI YACGTR 3 cut(s) 1687, 2374, 2706
BsaHI GRCGYC 1 cut(s) 1837
BsaI GGTCTC 2 cut(s) 1154, 2926
BsaJI CCNNGG 7 cut(s) 24, 280, 1164, 1238, 1661, 1999, 2457
BsaWI WCCGGW 1 cut(s) 1505
BsaXI ACNNNNNCTCC 4 cut(s) 648, 678, 2358, 2388
Bsc4I CCNNNNNNNGG 6 cut(s) 647, 1451, 1953, 2231, 2683, 2697
Bse118I RCCGGY 2 cut(s) 33, 3154
Bse3DI GCAATG 1 cut(s) 1850
BseAI TCCGGA 1 cut(s) 1505
BseBI CCWGG 3 cut(s) 2001, 2458, 3196
BseDI CCNNGG 7 cut(s) 24, 280, 1164, 1238, 1661, 1999, 2457
BseLI CCNNNNNNNGG 6 cut(s) 647, 1451, 1953, 2231, 2683, 2697
BseMI GCAATG 1 cut(s) 1850
BseRI GAGGAG 2 cut(s) 182, 631
BseXI GCAGC 9 cut(s) 436, 493, 1592, 1747, 1969, 2038, 2537, 3134, 3176
BsgI GTGCAG 3 cut(s) 500, 1709, 2037
Bsh1236I CGCG 2 cut(s) 686, 1127
Bsh1285I CGRYCG 1 cut(s) 296
BshFI GGCC 3 cut(s) 37, 1307, 2465
BshNI GGYRCC 1 cut(s) 195
BsiEI CGRYCG 1 cut(s) 296
BsiHKAI GWGCWC 3 cut(s) 472, 573, 2582
BsiHKCI CYCGRG 1 cut(s) 736
BsiSI CCGG 5 cut(s) 24, 34, 641, 1506, 3155
BsiWI CGTACG 1 cut(s) 2706
BslFI GGGAC 3 cut(s) 202, 858, 1848
BslI CCNNNNNNNGG 6 cut(s) 647, 1451, 1953, 2231, 2683, 2697
BsmAI GTCTC 8 cut(s) 56, 93, 481, 1154, 1717, 2237, 2561, 2926
BsmFI GGGAC 3 cut(s) 202, 858, 1848
BsmI GAATGC 2 cut(s) 772, 1067
BsnI GGCC 3 cut(s) 37, 1307, 2465
Bso31I GGTCTC 2 cut(s) 1154, 2926
BsoBI CYCGRG 1 cut(s) 736
Bsp119I TTCGAA 1 cut(s) 121
Bsp1286I GDGCHC 3 cut(s) 472, 573, 2582
Bsp13I TCCGGA 1 cut(s) 1505
Bsp1407I TGTACA 1 cut(s) 2793
Bsp1720I GCTNAGC 1 cut(s) 471
Bsp19I CCATGG 1 cut(s) 1164
BspANI GGCC 3 cut(s) 37, 1307, 2465
BspEI TCCGGA 1 cut(s) 1505
BspFNI CGCG 2 cut(s) 686, 1127
BspLI GGNNCC 3 cut(s) 197, 620, 2688
BspMAI CTGCAG 1 cut(s) 1927
BspMI ACCTGC 2 cut(s) 1646, 1810
BspOI GCTAGC 1 cut(s) 339
BspQI GCTCTTC 1 cut(s) 2088
BspT104I TTCGAA 1 cut(s) 121
BspT107I GGYRCC 1 cut(s) 195
BspTNI GGTCTC 2 cut(s) 1154, 2926
BsrBI CCGCTC 2 cut(s) 628, 2066
BsrDI GCAATG 1 cut(s) 1850
BsrFI RCCGGY 2 cut(s) 33, 3154
BsrGI TGTACA 1 cut(s) 2793
BssAI RCCGGY 2 cut(s) 33, 3154
BssECI CCNNGG 7 cut(s) 24, 280, 1164, 1238, 1661, 1999, 2457
BssNI GRCGYC 1 cut(s) 1837
BssT1I CCWWGG 3 cut(s) 1164, 1238, 1661
Bst2UI CCWGG 3 cut(s) 2001, 2458, 3196
Bst4CI ACNGT 7 cut(s) 200, 284, 841, 933, 1709, 1787, 2857
Bst6I CTCTTC 7 cut(s) 277, 866, 954, 2064, 2088, 2235, 3184
BstACI GRCGYC 1 cut(s) 1837
BstAPI GCANNNNNTGC 1 cut(s) 1724
BstAUI TGTACA 1 cut(s) 2793
BstBAI YACGTR 3 cut(s) 1687, 2374, 2706
BstBI TTCGAA 1 cut(s) 121
BstC8I GCNNGC 6 cut(s) 333, 337, 468, 870, 2467, 2987
BstDSI CCRYGG 2 cut(s) 280, 1164
BstENI CCTNNNNNAGG 2 cut(s) 1449, 1951
BstFNI CGCG 2 cut(s) 686, 1127
BstH2I RGCGCY 2 cut(s) 1606, 2889
BstHHI GCGC 4 cut(s) 1127, 1605, 2809, 2888
BstMAI GTCTC 8 cut(s) 56, 93, 481, 1154, 1717, 2237, 2561, 2926
BstMCI CGRYCG 1 cut(s) 296
BstMWI GCNNNNNNNGC 8 cut(s) 472, 481, 577, 677, 1724, 2051, 2462, 3164
BstNI CCWGG 3 cut(s) 2001, 2458, 3196
BstNSI RCATGY 5 cut(s) 466, 479, 886, 1391, 1874
BstPAI GACNNNNGTC 1 cut(s) 2271
BstSCI CCNGG 5 cut(s) 23, 640, 1999, 2456, 3194
BstSFI CTRYAG 4 cut(s) 312, 1113, 1449, 1923
BstSNI TACGTA 1 cut(s) 2706
BstUI CGCG 2 cut(s) 686, 1127
BstV1I GCAGC 9 cut(s) 436, 493, 1592, 1747, 1969, 2038, 2537, 3134, 3176
BstV2I GAAGAC 2 cut(s) 749, 1629
BstX2I RGATCY 4 cut(s) 637, 1672, 2116, 2686
BstXI CCANNNNNNTGG 1 cut(s) 1902
BstYI RGATCY 4 cut(s) 637, 1672, 2116, 2686
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 3 cut(s) 37, 1307, 2465
BtgI CCRYGG 2 cut(s) 280, 1164
BtsI GCAGTG 1 cut(s) 1986
BtsIMutI CAGTG 4 cut(s) 846, 1714, 1792, 1986
BveI ACCTGC 2 cut(s) 1646, 1810
Cac8I GCNNGC 6 cut(s) 333, 337, 468, 870, 2467, 2987
CaiI CAGNNNCTG 4 cut(s) 490, 1931, 1988, 2804
CfoI GCGC 4 cut(s) 1127, 1605, 2809, 2888
Cfr10I RCCGGY 2 cut(s) 33, 3154
Cfr13I GGNCC 3 cut(s) 1565, 1658, 3198
CseI GACGC 1 cut(s) 1845
CsiI ACCWGGT 1 cut(s) 3194
Eam1104I CTCTTC 7 cut(s) 277, 866, 954, 2064, 2088, 2235, 3184
EarI CTCTTC 7 cut(s) 277, 866, 954, 2064, 2088, 2235, 3184
EciI GGCGGA 2 cut(s) 10, 31
Eco105I TACGTA 1 cut(s) 2706
Eco130I CCWWGG 3 cut(s) 1164, 1238, 1661
Eco147I AGGCCT 1 cut(s) 1307
Eco31I GGTCTC 2 cut(s) 1154, 2926
Eco32I GATATC 1 cut(s) 2292
Eco47I GGWCC 3 cut(s) 1565, 1658, 3198
Eco47III AGCGCT 1 cut(s) 2887
Eco57I CTGAAG 3 cut(s) 967, 1545, 1590
Eco88I CYCGRG 1 cut(s) 736
EcoNI CCTNNNNNAGG 2 cut(s) 1449, 1951
EcoRI GAATTC 1 cut(s) 942
EcoRII CCWGG 3 cut(s) 1999, 2456, 3194
EcoRV GATATC 1 cut(s) 2292
EcoT14I CCWWGG 3 cut(s) 1164, 1238, 1661
ErhI CCWWGG 3 cut(s) 1164, 1238, 1661
FalI AAGNNNNNCTT 4 cut(s) 156, 188, 1482, 1514
FaqI GGGAC 3 cut(s) 202, 858, 1848
FauI CCCGC 3 cut(s) 445, 781, 2870
FbaI TGATCA 1 cut(s) 492
FblI GTMKAC 1 cut(s) 3134
FspBI CTAG 8 cut(s) 141, 192, 336, 443, 635, 731, 2553, 2904
FspI TGCGCA 1 cut(s) 2808
GlaI GCGC 4 cut(s) 1126, 1604, 2808, 2887
GsuI CTGGAG 3 cut(s) 903, 1965, 2874
HaeII RGCGCY 2 cut(s) 1606, 2889
HaeIII GGCC 3 cut(s) 37, 1307, 2465
HapII CCGG 5 cut(s) 24, 34, 641, 1506, 3155
HgaI GACGC 1 cut(s) 1845
HhaI GCGC 4 cut(s) 1127, 1605, 2809, 2888
Hin1I GRCGYC 1 cut(s) 1837
Hin6I GCGC 4 cut(s) 1125, 1603, 2807, 2886
HinP1I GCGC 4 cut(s) 1125, 1603, 2807, 2886
HincII GTYRAC 2 cut(s) 74, 3135
HindII GTYRAC 2 cut(s) 74, 3135
HindIII AAGCTT 2 cut(s) 329, 1633
HpaII CCGG 5 cut(s) 24, 34, 641, 1506, 3155
HphI GGTGA 6 cut(s) 791, 803, 1421, 1512, 2419, 2558
Hpy166II GTNNAC 6 cut(s) 74, 232, 1070, 2796, 3135, 3173
Hpy8I GTNNAC 6 cut(s) 74, 232, 1070, 2796, 3135, 3173
HpyAV CCTTC 8 cut(s) 48, 991, 1297, 1455, 2319, 2601, 2885, 2962
HpyCH4III ACNGT 7 cut(s) 200, 284, 841, 933, 1709, 1787, 2857
HpyCH4IV ACGT 5 cut(s) 1029, 1686, 2373, 2532, 2705
HpyF10VI GCNNNNNNNGC 8 cut(s) 472, 481, 577, 677, 1724, 2051, 2462, 3164
HpySE526I ACGT 5 cut(s) 1029, 1686, 2373, 2532, 2705
Hsp92I GRCGYC 1 cut(s) 1837
HspAI GCGC 4 cut(s) 1125, 1603, 2807, 2886
Kpn2I TCCGGA 1 cut(s) 1505
KpnI GGTACC 1 cut(s) 199
Ksp22I TGATCA 1 cut(s) 492
LguI GCTCTTC 1 cut(s) 2088
LmnI GCTCC 7 cut(s) 169, 568, 618, 656, 1129, 2063, 2893
Lsp1109I GCAGC 9 cut(s) 436, 493, 1592, 1747, 1969, 2038, 2537, 3134, 3176
LweI GCATC 8 cut(s) 70, 410, 658, 775, 1579, 2133, 2351, 2935
MabI ACCWGGT 1 cut(s) 3194
MaeI CTAG 8 cut(s) 141, 192, 336, 443, 635, 731, 2553, 2904
MaeII ACGT 5 cut(s) 1029, 1686, 2373, 2532, 2705
MaeIII GTNAC 7 cut(s) 59, 284, 835, 1117, 2369, 2659, 2932
MbiI CCGCTC 2 cut(s) 628, 2066
MfeI CAATTG 1 cut(s) 2009
MflI RGATCY 4 cut(s) 637, 1672, 2116, 2686
MhlI GDGCHC 3 cut(s) 472, 573, 2582
MmeI TCCRAC 4 cut(s) 93, 480, 2397, 2664
MroI TCCGGA 1 cut(s) 1505
MroXI GAANNNNTTC 3 cut(s) 980, 1794, 2352
MslI CAYNNNNRTG 1 cut(s) 2561
MspA1I CMGCKG 3 cut(s) 218, 2528, 3167
MspI CCGG 5 cut(s) 24, 34, 641, 1506, 3155
MspR9I CCNGG 5 cut(s) 25, 642, 2001, 2458, 3196
MunI CAATTG 1 cut(s) 2009
Mva1269I GAATGC 2 cut(s) 772, 1067
MvaI CCWGG 3 cut(s) 2001, 2458, 3196
MvnI CGCG 2 cut(s) 686, 1127
MwoI GCNNNNNNNGC 8 cut(s) 472, 481, 577, 677, 1724, 2051, 2462, 3164
NciI CCSGG 2 cut(s) 25, 642
NcoI CCATGG 1 cut(s) 1164
NheI GCTAGC 1 cut(s) 335
NlaIV GGNNCC 3 cut(s) 197, 620, 2688
NmuCI GTSAC 2 cut(s) 2369, 2659
NsbI TGCGCA 1 cut(s) 2808
NspI RCATGY 5 cut(s) 466, 479, 886, 1391, 1874
NspV TTCGAA 1 cut(s) 121
OliI CACNNNNGTG 1 cut(s) 2561
PaeR7I CTCGAG 1 cut(s) 736
PaqCI CACCTGC 1 cut(s) 1810
PceI AGGCCT 1 cut(s) 1307
PciI ACATGT 1 cut(s) 1870
PciSI GCTCTTC 1 cut(s) 2088
PctI GAATGC 2 cut(s) 772, 1067
PdmI GAANNNNTTC 3 cut(s) 980, 1794, 2352
PfeI GAWTC 8 cut(s) 118, 751, 829, 949, 998, 1248, 1379, 2831
Pfl23II CGTACG 1 cut(s) 2706
PflFI GACNNNGTC 1 cut(s) 1709
PflMI CCANNNNNTGG 2 cut(s) 2683, 2697
Ple19I CGATCG 1 cut(s) 296
Ppu21I YACGTR 3 cut(s) 1687, 2374, 2706
PscI ACATGT 1 cut(s) 1870
PshAI GACNNNNGTC 1 cut(s) 2271
PshBI ATTAAT 1 cut(s) 2823
PsiI TTATAA 1 cut(s) 897
Psp6I CCWGG 3 cut(s) 1999, 2456, 3194
PspGI CCWGG 3 cut(s) 1999, 2456, 3194
PspLI CGTACG 1 cut(s) 2706
PspN4I GGNNCC 3 cut(s) 197, 620, 2688
PspPI GGNCC 3 cut(s) 1565, 1658, 3198
PspXI VCTCGAGB 1 cut(s) 736
PstI CTGCAG 1 cut(s) 1927
PstNI CAGNNNCTG 4 cut(s) 490, 1931, 1988, 2804
PsuI RGATCY 4 cut(s) 637, 1672, 2116, 2686
PsyI GACNNNGTC 1 cut(s) 1709
PvuI CGATCG 1 cut(s) 296
PvuII CAGCTG 3 cut(s) 218, 2528, 3167
RseI CAYNNNNRTG 1 cut(s) 2561
SalI GTCGAC 1 cut(s) 3133
SapI GCTCTTC 1 cut(s) 2088
Sau96I GGNCC 3 cut(s) 1565, 1658, 3198
ScrFI CCNGG 5 cut(s) 25, 642, 2001, 2458, 3196
SduI GDGCHC 3 cut(s) 472, 573, 2582
SexAI ACCWGGT 1 cut(s) 3194
SfaNI GCATC 8 cut(s) 70, 410, 658, 775, 1579, 2133, 2351, 2935
SfcI CTRYAG 4 cut(s) 312, 1113, 1449, 1923
Sfr274I CTCGAG 1 cut(s) 736
SfuI TTCGAA 1 cut(s) 121
SinI GGWCC 3 cut(s) 1565, 1658, 3198
SlaI CTCGAG 1 cut(s) 736
SmiMI CAYNNNNRTG 1 cut(s) 2561
SmlI CTYRAG 5 cut(s) 719, 736, 907, 1183, 3159
SmoI CTYRAG 5 cut(s) 719, 736, 907, 1183, 3159
SnaBI TACGTA 1 cut(s) 2706
SseBI AGGCCT 1 cut(s) 1307
SspI AATATT 3 cut(s) 1296, 3081, 3105
SspMI CTAG 8 cut(s) 141, 192, 336, 443, 635, 731, 2553, 2904
StuI AGGCCT 1 cut(s) 1307
StyD4I CCNGG 5 cut(s) 23, 640, 1999, 2456, 3194
StyI CCWWGG 3 cut(s) 1164, 1238, 1661
TaaI ACNGT 7 cut(s) 200, 284, 841, 933, 1709, 1787, 2857
TaiI ACGT 5 cut(s) 1032, 1689, 2376, 2535, 2708
TatI WGTACW 4 cut(s) 773, 2758, 2793, 2909
TauI GCSGC 2 cut(s) 631, 3128
TfiI GAWTC 8 cut(s) 118, 751, 829, 949, 998, 1248, 1379, 2831
TscAI CASTG 4 cut(s) 846, 1714, 1792, 1993
TseFI GTSAC 2 cut(s) 2369, 2659
TseI GCWGC 9 cut(s) 449, 481, 1605, 1760, 1957, 2051, 2525, 3122, 3164
Tsp45I GTSAC 2 cut(s) 2369, 2659
TspDTI ATGAA 8 cut(s) 251, 1305, 1337, 1459, 2028, 2316, 2345, 3037
TspGWI ACGGA 2 cut(s) 78, 991
TspRI CASTG 4 cut(s) 846, 1714, 1792, 1993
Tth111I GACNNNGTC 1 cut(s) 1709
Van91I CCANNNNNTGG 2 cut(s) 2683, 2697
VpaK11BI GGWCC 3 cut(s) 1565, 1658, 3198
VspI ATTAAT 1 cut(s) 2823
XagI CCTNNNNNAGG 2 cut(s) 1449, 1951
XbaI TCTAGA 4 cut(s) 442, 634, 730, 2903
XceI RCATGY 5 cut(s) 466, 479, 886, 1391, 1874
XcmI CCANNNNNNNNNTGG 1 cut(s) 3028
XhoI CTCGAG 1 cut(s) 736
XmiI GTMKAC 1 cut(s) 3134
XmnI GAANNNNTTC 3 cut(s) 980, 1794, 2352
XspI CTAG 8 cut(s) 141, 192, 336, 443, 635, 731, 2553, 2904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.