FvH4_5g28760

Conserved oligomeric Golgi complex subunit 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005295
Physical Location & Seq
Forward (+)
19908539 .. 19916523
7985 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28760.t2

Sequence Viewer

Length: 3018 bp
ATGAGAGTGAGCAATGAGGCGGAGGCGCTGTTCCGGACGAAAGGAATCAGCGAGATCCGAAATGTGGAATCGGCGACGCGTTTGCAGATCCAAGCGAAGAAGGAGGAGCTCCGTCAACTGGTGGGCACACGGTACCGTGACCTCATCGACTCGGCCGACTCCATCGTCCTGATGAAGCGGTCCTCCCTCTCCATCTCCCGAAACCTCTCCTCCGTCCACCACTCCATCCTCTCTCTTTCGCGTTCCCATGATGAGGAATCTAAGCCCTCCGATGGCGATGGCTCTAATGGCAATGGCATCTACGCCGTCGCCTGCCGCGTCAAGTACCTGGTGGACACCCCGGAGAACATATGGGGCTGCCTCGACGAGTCCATGTTCCTCGAGTCCGCCGCCCGCTACGTCCGTGCCCGCCACGTCCACTCCACTCTCACCACCCTCCATAACTCCTTCCTCTCTCCCTTCCCTCTCCTCCACCACCAATGGCAGATCGTCGACACCTTCAAGTCCCAGATCTCCCAGCGCTCCCGCGACCTCCTCTCCGACCACCGCCTCTCCCACCTCCCCGTCTCCTCCTTCGCCGACGCCTTGGCCGCCCTCGCTCTCATCGACGACCTCTCCCCCCGCCACGTCCTCTCCCTCTTCCTCCACACCCGCAAGGCCTGGCTCTCCCACATTCTCAACGCCGCCGCCGATGACGACGTCGTCTCCGTGCTCTGCCAGGCCCTCAGGGTCATTCAGCTCACTGTGGGCCAGGTCGGGGAACTCTTTCTCCGTGTTTTCAACGACATGCCTCTCTTTTACAAGGTCGTCTTGGCCTGCCCTCCTGCGTCCCAACTCTTTGGCGGCATTCCCAACCCCGATGAGGAGGTCAAGCTGTGGAACTCCTTCAGGGACAACTTGGAGTCCGCCATGGGAATACTCGACAAAGACTACATCGCCGCTGCTTCTTCTTCATGGCTTAGCCACTGCGGAGCTCAGATGCTCCACCAGATTAATGGCAGGTATCTCATCGATGCTATTGCCAGCGGCAAAGAGCTTGCCGCTGCTGAGAGCTTGATAAGGGAGACTATGAACAGCAAAGAGGTCCTAGAAGGGAGCTTGGAGTGGCTCAAGAAAGTTTTCGGCTCCCAAATTGACCTGCCATGGAGTCGAATGAGTGAACTTGTTCTGGGAGATGATTCCGACCTCTGGGACAGTATCTTTGAGAGTGGATTTGTTGGGAGGATGAAGGTGATTGTGGACTCCCGGTTTGACAAGCTCAAGACGGCTGCCAGTATTAATCATGGCCAGGCCGTTGATTTCCTAGGGGCAGGTGGTGGAGTTTGGTTCTTAGAATCCAAATCAAACAATAAGAAGGCTGGTTCTCTGCCATGTGAAGAGAATTGTCTCAATTTCTACTTTGGTCCTCAAGTTAGCGATATTAGGGATGCGGTGGACAGCAGCTGTCAGGATGTGCTTGATGACCTCCTCTCTTTTCTGGAGTCTCCAAAGGCAGCTGTTAGATTAAAGAATCTGGTGCCCTATTTACAAGACAAGTGTTTCCAAACTGTATCCCTCATATTGGAGCAACTGAGTAGTGAACTTGATAATTTGGAAAAGGGCAGCAACAAGCACAAGCTGGTAACCGTCGAAAGGGCTCTTTTCATCGGTAGACTCTTGTTTGCGTTGCAGAATCACTCCAAACACATTCCCTTAGTACTTGGCCCACCAAGGTCTTGGGTGAATGAAGCTGAGTCTACAGTTTTTGACAAATTACCATCCTTACTGAGACAGTCAAGAGCACCCACCGATTCTCATGTGCTTGATAGCCCCCTCAGCACTAAAAGACACACTTCATCTGCTACTGCCGCACTGCTTGGAGCCAGTCAAAGTGCAAGCCCTAAACTTGAAGAGCTTAATGTAACTATGCGAAATCTCCGAATTAGAGCACATGCTTTCTGGATGACTTGGCTATCTGATGAGCTCTCAGTTATACTTTCCCGTGATCTAGAAAAGGACAATGCTTTGTCATCCTCAACTCCCCTGAGGGGTTGGGAAGAGACAGTAGTTAGGCAAGAACAGTCTGATGATGACCAATCAGAGATGAAAATATGGCTCCCATCCATGCCTTCTCTCTACATTGTTTCGTTTCTGTTTCGTGTATGTGAAGAAGTTCACCGGATAGGAGGTCATGTTCTTGACAAAATAATTTTGCAAAAATTTGCGTTAAGACTATTAGAAAAGGTTCTTGGCATTTATGGCGACTTCCTTTCAAATCTAGATGCTGGTGGGTCTCAAGTGTCAGAAAAAGGAGTCTTGCAGATTTTGTTGGACTTGAGATTTGTTTTTGATGTCCTCTCTGGGGGTGATTCTAATTTAAATGAGGAGCGGAAGACAAAGAGTACTTTCAGAAGGAAGCAGGACCAAAGCCGTATGAAGTCAGCTACTGGAGAGCGATTTGATGGGTTGATACATAGATTTTCACAAAGATTGGATCCCATAGACTGGCTAACGTATGAGCCATATCTCTGGGAAAATGAGAAGCAGTCCTACCTCCGGCATGCCGTCCTTTTTGGATTCTTTGTGCAACTTAATCGCATGTACACAGACACTGTTCAAAAACTTCCTACTAATTCAGAGTCCAATGTCATGAGATGCTCTTCAGTTCCTCGCTTTAAGTACCTTCCAATCAGTGCCCCAGCATTGTCTTCAAGAGGAACAGCTGCTAAGAAATCCATTGCAACATCTTCTGATGATATTTCTTCAAGAAGTACCTGGAAAGCTTATACAAATGGAGAGCTGTCCAGAAGTATGGATTTGGATGATAACTCAAGTTTTGGGGTTGCAGTACCAATCTTAAAATCTTTTATGCAGGCTGGAAGTAGATTTGGAGAGAGCACAATGAAACTGGGATCCATGCTAACAGATGGGCAAGTGGGAATATTCAAGGATAGATCCATGTCGACATTTGGTGACATTCTACCTGCCCATGCTGCAGGTCTCCTTTCATCATTCACAGCTTCCAGATCAGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps51

Protein Analysis

1006

Amino Acids

112.19

Weight (kDa)

6.28

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS51_Exo84_N PF08700 6 - 76 1.5e-13 Vps51/EXO84/COG1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1301
AasI GACNNNNNNGTC 2 cut(s) 164, 701
AatII GACGTC 1 cut(s) 702
Acc36I ACCTGC 5 cut(s) 990, 1146, 1301, 2966, 2971
Acc65I GGTACC 1 cut(s) 132
AccB1I GGYRCC 2 cut(s) 132, 1516
AccB7I CCANNNNNTGG 1 cut(s) 2484
AccBSI CCGCTC 1 cut(s) 2368
AccI GTMKAC 4 cut(s) 492, 1651, 1736, 2942
AccII CGCG 4 cut(s) 79, 241, 318, 528
AccIII TCCGGA 1 cut(s) 33
AclWI GGATC 7 cut(s) 49, 82, 2468, 2481, 2886, 2899, 2928
AcoI YGGCCR 3 cut(s) 153, 588, 1285
AcsI RAATTY 1 cut(s) 2198
AcuI CTGAAG 2 cut(s) 871, 2625
AcyI GRCGYC 2 cut(s) 582, 699
AfaI GTAC 8 cut(s) 134, 326, 1698, 2383, 2582, 2660, 2752, 2829
AfeI AGCGCT 1 cut(s) 521
AflIII ACRYGT 1 cut(s) 77
AgsI TTSAA 8 cut(s) 502, 781, 1889, 2253, 2597, 2691, 2745, 2926
AjiI CACGTC 2 cut(s) 415, 628
AjnI CCWGG 6 cut(s) 327, 659, 717, 750, 1287, 2753
AjuI GAANNNNNNNTTGG 2 cut(s) 2211, 2243
AloI GAACNNNNNNTCC 6 cut(s) 14, 46, 753, 785, 2157, 2189
Alw21I GWGCWC 7 cut(s) 111, 714, 976, 1783, 1930, 1965, 2879
Alw26I GTCTC 9 cut(s) 571, 709, 1058, 1391, 1488, 1762, 2033, 2277, 2984
AlwI GGATC 7 cut(s) 49, 82, 2468, 2481, 2886, 2899, 2928
AlwNI CAGNNNCTG 3 cut(s) 1443, 2426, 2591
Ama87I CYCGRG 1 cut(s) 380
Aor13HI TCCGGA 1 cut(s) 33
Aor51HI AGCGCT 1 cut(s) 521
AoxI GGCC 9 cut(s) 153, 588, 657, 720, 748, 813, 1285, 1290, 1702
ApeKI GCWGC 9 cut(s) 357, 941, 1043, 1268, 1440, 1493, 1602, 2702, 2972
ApoI RAATTY 1 cut(s) 2198
ArsI GACNNNNNNTTYG 6 cut(s) 1184, 1216, 1232, 1264, 2303, 2335
AseI ATTAAT 2 cut(s) 993, 1278
Asp700I GAANNNNTTC 6 cut(s) 765, 884, 1118, 1164, 2151, 2223
Asp718I GGTACC 1 cut(s) 132
AspA2I CCTAGG 1 cut(s) 1303
AspLEI GCGC 2 cut(s) 28, 522
AspS9I GGNCC 7 cut(s) 180, 721, 748, 1084, 1403, 1703, 2401
AsuC2I CCSGG 2 cut(s) 341, 1246
AsuHPI GGTGA 6 cut(s) 421, 1243, 1732, 2147, 2357, 2963
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 4 cut(s) 180, 1084, 1403, 2401
AvrII CCTAGG 1 cut(s) 1303
AxyI CCTNAGG 2 cut(s) 725, 2024
BaeGI GKGCMC 4 cut(s) 128, 409, 1521, 2677
BalI TGGCCA 1 cut(s) 1287
BamHI GGATCC 2 cut(s) 2473, 2891
BanI GGYRCC 2 cut(s) 132, 1516
BanII GRGCYC 4 cut(s) 111, 976, 1639, 1965
BarI GAAGNNNNNNTAC 2 cut(s) 2513, 2545
BbsI GAAGAC 2 cut(s) 2378, 2679
Bbv12I GWGCWC 7 cut(s) 111, 714, 976, 1783, 1930, 1965, 2879
BbvCI CCTCAGC 1 cut(s) 1814
BbvI GCAGC 9 cut(s) 344, 928, 1030, 1255, 1452, 1505, 1614, 2689, 2959
BccI CCATC 9 cut(s) 170, 200, 233, 266, 272, 1765, 2107, 2435, 2900
BceAI ACGGC 5 cut(s) 290, 1277, 1281, 2394, 2528
BcgI CGANNNNNNTGC 6 cut(s) 64, 98, 1001, 1035, 2555, 2589
BciT130I CCWGG 6 cut(s) 329, 661, 719, 752, 1289, 2755
BciVI GTATCC 1 cut(s) 1561
BcnI CCSGG 2 cut(s) 341, 1246
BcoDI GTCTC 9 cut(s) 571, 709, 1058, 1391, 1488, 1762, 2033, 2277, 2984
BfaI CTAG 4 cut(s) 1088, 1304, 1988, 2258
BfmI CTRYAG 2 cut(s) 1737, 2973
BfoI RGCGCY 2 cut(s) 29, 523
BfuAI ACCTGC 5 cut(s) 990, 1146, 1301, 2966, 2971
BfuI GTATCC 1 cut(s) 1561
BglII AGATCT 1 cut(s) 510
BlnI CCTAGG 1 cut(s) 1303
BlpI GCTNAGC 1 cut(s) 959
BmcAI AGTACT 2 cut(s) 1698, 2383
Bme1390I CCNGG 8 cut(s) 329, 341, 661, 719, 752, 1246, 1289, 2755
Bme18I GGWCC 4 cut(s) 180, 1084, 1403, 2401
BmeT110I CYCGRG 1 cut(s) 380
BmgBI CACGTC 2 cut(s) 415, 628
BmgT120I GGNCC 7 cut(s) 180, 721, 748, 1084, 1403, 1703, 2401
BmiI GGNNCC 7 cut(s) 134, 1126, 1518, 1861, 2096, 2475, 2893
BmrFI CCNGG 8 cut(s) 329, 341, 661, 719, 752, 1246, 1289, 2755
BmrI ACTGGG 1 cut(s) 2897
BmsI GCATC 6 cut(s) 306, 969, 1003, 1417, 2251, 2624
BmuI ACTGGG 1 cut(s) 2897
BpiI GAAGAC 2 cut(s) 2378, 2679
BpmI CTGGAG 2 cut(s) 1499, 2448
Bpu10I CCTNAGC 1 cut(s) 1814
Bpu1102I GCTNAGC 1 cut(s) 959
BpuEI CTTGAG 6 cut(s) 1094, 1244, 1392, 2259, 2335, 2794
BpuMI CCSGG 2 cut(s) 341, 1246
Bsa29I ATCGAT 1 cut(s) 1011
BsaHI GRCGYC 2 cut(s) 582, 699
BsaI GGTCTC 2 cut(s) 2277, 2984
BsaJI CCNNGG 6 cut(s) 339, 585, 909, 1142, 1303, 1709
BsaWI WCCGGW 2 cut(s) 33, 2157
Bse1I ACTGG 6 cut(s) 123, 1272, 1863, 2431, 2489, 2892
Bse21I CCTNAGG 2 cut(s) 725, 2024
Bse3DI GCAATG 3 cut(s) 19, 298, 2715
BseAI TCCGGA 1 cut(s) 33
BseBI CCWGG 6 cut(s) 329, 661, 719, 752, 1289, 2755
BseCI ATCGAT 1 cut(s) 1011
BseDI CCNNGG 6 cut(s) 339, 585, 909, 1142, 1303, 1709
BseGI GGATG 9 cut(s) 225, 1230, 1432, 1456, 1757, 1947, 2009, 2099, 2806
BseMI GCAATG 3 cut(s) 19, 298, 2715
BseMII CTCAG 9 cut(s) 739, 989, 1038, 1562, 1722, 1757, 1828, 1980, 2015
BseNI ACTGG 6 cut(s) 123, 1272, 1863, 2431, 2489, 2892
BseRI GAGGAG 8 cut(s) 119, 199, 458, 524, 559, 878, 1457, 2378
BseSI GKGCMC 4 cut(s) 128, 409, 1521, 2677
BseX3I CGGCCG 1 cut(s) 153
BseXI GCAGC 9 cut(s) 344, 928, 1030, 1255, 1452, 1505, 1614, 2689, 2959
BseYI CCCAGC 2 cut(s) 516, 2677
Bsh1236I CGCG 4 cut(s) 79, 241, 318, 528
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 9 cut(s) 155, 590, 659, 722, 750, 815, 1287, 1292, 1704
BshNI GGYRCC 2 cut(s) 132, 1516
BshVI ATCGAT 1 cut(s) 1011
BsiEI CGRYCG 1 cut(s) 156
BsiHKAI GWGCWC 7 cut(s) 111, 714, 976, 1783, 1930, 1965, 2879
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 5 cut(s) 34, 341, 1246, 2158, 2536
BslFI GGGAC 4 cut(s) 490, 814, 905, 1205
BsmAI GTCTC 9 cut(s) 571, 709, 1058, 1391, 1488, 1762, 2033, 2277, 2984
BsmBI CGTCTC 2 cut(s) 571, 709
BsmFI GGGAC 4 cut(s) 490, 814, 905, 1205
BsmI GAATGC 1 cut(s) 846
BsnI GGCC 9 cut(s) 155, 590, 659, 722, 750, 815, 1287, 1292, 1704
Bso31I GGTCTC 2 cut(s) 2277, 2984
BsoBI CYCGRG 1 cut(s) 380
Bsp13I TCCGGA 1 cut(s) 33
Bsp1407I TGTACA 1 cut(s) 2580
Bsp143I GATC 9 cut(s) 54, 87, 486, 510, 1984, 2473, 2891, 2933, 3005
Bsp1720I GCTNAGC 1 cut(s) 959
Bsp19I CCATGG 2 cut(s) 909, 1142
BspANI GGCC 9 cut(s) 155, 590, 659, 722, 750, 815, 1287, 1292, 1704
BspCNI CTCAG 9 cut(s) 738, 988, 1039, 1563, 1723, 1758, 1827, 1979, 2016
BspDI ATCGAT 1 cut(s) 1011
BspEI TCCGGA 1 cut(s) 33
BspFNI CGCG 4 cut(s) 79, 241, 318, 528
BspHI TCATGA 1 cut(s) 2628
BspLI GGNNCC 7 cut(s) 134, 1126, 1518, 1861, 2096, 2475, 2893
BspMAI CTGCAG 1 cut(s) 2977
BspMI ACCTGC 5 cut(s) 990, 1146, 1301, 2966, 2971
BspPI GGATC 7 cut(s) 49, 82, 2468, 2481, 2886, 2899, 2928
BspQI GCTCTTC 2 cut(s) 1884, 2644
BspT107I GGYRCC 2 cut(s) 132, 1516
BspTNI GGTCTC 2 cut(s) 2277, 2984
BsrBI CCGCTC 1 cut(s) 2368
BsrDI GCAATG 3 cut(s) 19, 298, 2715
BsrGI TGTACA 1 cut(s) 2580
BsrI ACTGG 6 cut(s) 123, 1272, 1863, 2431, 2489, 2892
BssECI CCNNGG 6 cut(s) 339, 585, 909, 1142, 1303, 1709
BssMI GATC 9 cut(s) 54, 87, 486, 510, 1984, 2473, 2891, 2933, 3005
BssNI GRCGYC 2 cut(s) 582, 699
BssT1I CCWWGG 5 cut(s) 585, 909, 1142, 1303, 1709
Bst2UI CCWGG 6 cut(s) 329, 661, 719, 752, 1289, 2755
Bst6I CTCTTC 5 cut(s) 644, 1371, 1884, 2031, 2644
BstACI GRCGYC 2 cut(s) 582, 699
BstAUI TGTACA 1 cut(s) 2580
BstC8I GCNNGC 9 cut(s) 313, 394, 409, 817, 1024, 1038, 1876, 2541, 2853
BstDSI CCRYGG 2 cut(s) 909, 1142
BstEII GGTNACC 1 cut(s) 1621
BstF5I GGATG 9 cut(s) 225, 1230, 1432, 1456, 1757, 1947, 2009, 2099, 2806
BstFNI CGCG 4 cut(s) 79, 241, 318, 528
BstH2I RGCGCY 2 cut(s) 29, 523
BstHHI GCGC 2 cut(s) 28, 522
BstKTI GATC 9 cut(s) 57, 90, 489, 513, 1987, 2476, 2894, 2936, 3008
BstMAI GTCTC 9 cut(s) 571, 709, 1058, 1391, 1488, 1762, 2033, 2277, 2984
BstMBI GATC 9 cut(s) 54, 87, 486, 510, 1984, 2473, 2891, 2933, 3005
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 7 cut(s) 288, 590, 596, 1815, 1847, 2238, 2972
BstNI CCWGG 6 cut(s) 329, 661, 719, 752, 1289, 2755
BstNSI RCATGY 4 cut(s) 790, 1934, 2543, 2581
BstPI GGTNACC 1 cut(s) 1621
BstSCI CCNGG 8 cut(s) 327, 339, 659, 717, 750, 1244, 1287, 2753
BstSFI CTRYAG 2 cut(s) 1737, 2973
BstSLI GKGCMC 4 cut(s) 128, 409, 1521, 2677
BstUI CGCG 4 cut(s) 79, 241, 318, 528
BstV1I GCAGC 9 cut(s) 344, 928, 1030, 1255, 1452, 1505, 1614, 2689, 2959
BstV2I GAAGAC 2 cut(s) 2378, 2679
BstX2I RGATCY 6 cut(s) 54, 87, 510, 2473, 2891, 2933
BstXI CCANNNNNNTGG 5 cut(s) 839, 995, 1716, 2508, 2791
BstYI RGATCY 6 cut(s) 54, 87, 510, 2473, 2891, 2933
BstZI CGGCCG 1 cut(s) 153
Bsu15I ATCGAT 1 cut(s) 1011
Bsu36I CCTNAGG 2 cut(s) 725, 2024
BsuI GTATCC 1 cut(s) 1561
BsuRI GGCC 9 cut(s) 155, 590, 659, 722, 750, 815, 1287, 1292, 1704
BsuTUI ATCGAT 1 cut(s) 1011
BtgI CCRYGG 2 cut(s) 909, 1142
BtgZI GCGATG 2 cut(s) 291, 919
BtrI CACGTC 2 cut(s) 415, 628
BtsCI GGATG 9 cut(s) 225, 1230, 1432, 1456, 1757, 1947, 2009, 2099, 2806
BtsI GCAGTG 2 cut(s) 964, 1850
BtsIMutI CAGTG 5 cut(s) 741, 964, 1850, 2589, 2677
BveI ACCTGC 5 cut(s) 990, 1146, 1301, 2966, 2971
Cac8I GCNNGC 9 cut(s) 313, 394, 409, 817, 1024, 1038, 1876, 2541, 2853
CaiI CAGNNNCTG 3 cut(s) 1443, 2426, 2591
CciI TCATGA 1 cut(s) 2628
CfoI GCGC 2 cut(s) 28, 522
Cfr13I GGNCC 7 cut(s) 180, 721, 748, 1084, 1403, 1703, 2401
ClaI ATCGAT 1 cut(s) 1011
CseI GACGC 4 cut(s) 85, 307, 590, 816
CsiI ACCWGGT 1 cut(s) 327
Csp6I GTAC 8 cut(s) 133, 325, 1697, 2382, 2581, 2659, 2751, 2828
CviQI GTAC 8 cut(s) 133, 325, 1697, 2382, 2581, 2659, 2751, 2828
DpnI GATC 9 cut(s) 56, 89, 488, 512, 1986, 2475, 2893, 2935, 3007
DpnII GATC 9 cut(s) 54, 87, 486, 510, 1984, 2473, 2891, 2933, 3005
DraI TTTAAA 1 cut(s) 2358
DrdI GACNNNNNNGTC 2 cut(s) 164, 701
DseDI GACNNNNNNGTC 2 cut(s) 164, 701
EaeI YGGCCR 3 cut(s) 153, 588, 1285
EagI CGGCCG 1 cut(s) 153
Eam1104I CTCTTC 5 cut(s) 644, 1371, 1884, 2031, 2644
EarI CTCTTC 5 cut(s) 644, 1371, 1884, 2031, 2644
EciI GGCGGA 3 cut(s) 35, 376, 895
Ecl136II GAGCTC 3 cut(s) 109, 974, 1963
EclXI CGGCCG 1 cut(s) 153
Eco130I CCWWGG 5 cut(s) 585, 909, 1142, 1303, 1709
Eco147I AGGCCT 1 cut(s) 659
Eco24I GRGCYC 4 cut(s) 111, 976, 1639, 1965
Eco31I GGTCTC 2 cut(s) 2277, 2984
Eco47I GGWCC 4 cut(s) 180, 1084, 1403, 2401
Eco47III AGCGCT 1 cut(s) 521
Eco52I CGGCCG 1 cut(s) 153
Eco53kI GAGCTC 3 cut(s) 109, 974, 1963
Eco57I CTGAAG 2 cut(s) 871, 2625
Eco81I CCTNAGG 2 cut(s) 725, 2024
Eco88I CYCGRG 1 cut(s) 380
Eco91I GGTNACC 1 cut(s) 1621
EcoICRI GAGCTC 3 cut(s) 109, 974, 1963
EcoO109I RGGNCCY 2 cut(s) 721, 1084
EcoO65I GGTNACC 1 cut(s) 1621
EcoRII CCWGG 6 cut(s) 327, 659, 717, 750, 1287, 2753
EcoT14I CCWWGG 5 cut(s) 585, 909, 1142, 1303, 1709
EcoT38I GRGCYC 4 cut(s) 111, 976, 1639, 1965
ErhI CCWWGG 5 cut(s) 585, 909, 1142, 1303, 1709
Esp3I CGTCTC 2 cut(s) 571, 709
FalI AAGNNNNNCTT 4 cut(s) 794, 826, 1816, 1848
FaqI GGGAC 4 cut(s) 490, 814, 905, 1205
FauI CCCGC 5 cut(s) 401, 416, 533, 629, 659
FauNDI CATATG 1 cut(s) 350
FblI GTMKAC 4 cut(s) 492, 1651, 1736, 2942
FokI GGATG 9 cut(s) 212, 1237, 1439, 1463, 1744, 1954, 1996, 2086, 2813
FriOI GRGCYC 4 cut(s) 111, 976, 1639, 1965
FspBI CTAG 4 cut(s) 1088, 1304, 1988, 2258
GlaI GCGC 2 cut(s) 27, 521
GsaI CCCAGC 2 cut(s) 520, 2681
GsuI CTGGAG 2 cut(s) 1499, 2448
HaeII RGCGCY 2 cut(s) 29, 523
HaeIII GGCC 9 cut(s) 155, 590, 659, 722, 750, 815, 1287, 1292, 1704
HapII CCGG 5 cut(s) 34, 341, 1246, 2158, 2536
HgaI GACGC 4 cut(s) 85, 307, 590, 816
HhaI GCGC 2 cut(s) 28, 522
Hin1I GRCGYC 2 cut(s) 582, 699
Hin6I GCGC 2 cut(s) 26, 520
HinP1I GCGC 2 cut(s) 26, 520
HincII GTYRAC 3 cut(s) 116, 493, 2943
HindII GTYRAC 3 cut(s) 116, 493, 2943
HindIII AAGCTT 1 cut(s) 2760
HpaII CCGG 5 cut(s) 34, 341, 1246, 2158, 2536
HphI GGTGA 6 cut(s) 421, 1243, 1732, 2147, 2357, 2963
Hpy99I CGWCG 9 cut(s) 79, 311, 368, 494, 584, 611, 701, 704, 1631
HpyCH4IV ACGT 5 cut(s) 399, 414, 627, 699, 2492
HpyF10VI GCNNNNNNNGC 7 cut(s) 288, 590, 596, 1815, 1847, 2238, 2972
HpySE526I ACGT 5 cut(s) 399, 414, 627, 699, 2492
Hsp92I GRCGYC 2 cut(s) 582, 699
HspAI GCGC 2 cut(s) 26, 520
Kpn2I TCCGGA 1 cut(s) 33
KpnI GGTACC 1 cut(s) 136
Kzo9I GATC 9 cut(s) 54, 87, 486, 510, 1984, 2473, 2891, 2933, 3005
LguI GCTCTTC 2 cut(s) 1884, 2644
Lsp1109I GCAGC 9 cut(s) 344, 928, 1030, 1255, 1452, 1505, 1614, 2689, 2959
LweI GCATC 6 cut(s) 306, 969, 1003, 1417, 2251, 2624
MabI ACCWGGT 1 cut(s) 327
MaeI CTAG 4 cut(s) 1088, 1304, 1988, 2258
MaeII ACGT 5 cut(s) 399, 414, 627, 699, 2492
MaeIII GTNAC 4 cut(s) 137, 1621, 1900, 2951
MalI GATC 9 cut(s) 56, 89, 488, 512, 1986, 2475, 2893, 2935, 3007
MbiI CCGCTC 1 cut(s) 2368
MboI GATC 9 cut(s) 54, 87, 486, 510, 1984, 2473, 2891, 2933, 3005
MflI RGATCY 6 cut(s) 54, 87, 510, 2473, 2891, 2933
MlsI TGGCCA 1 cut(s) 1287
MluI ACGCGT 1 cut(s) 77
MluNI TGGCCA 1 cut(s) 1287
MmeI TCCRAC 3 cut(s) 564, 1206, 2289
Mox20I TGGCCA 1 cut(s) 1287
MroI TCCGGA 1 cut(s) 33
MroXI GAANNNNTTC 6 cut(s) 765, 884, 1118, 1164, 2151, 2223
MscI TGGCCA 1 cut(s) 1287
MseI TTAA 9 cut(s) 993, 1278, 1505, 1896, 2207, 2357, 2571, 2655, 2837
Msp20I TGGCCA 1 cut(s) 1287
MspA1I CMGCKG 6 cut(s) 941, 1026, 1043, 1443, 1496, 2702
MspI CCGG 5 cut(s) 34, 341, 1246, 2158, 2536
MspR9I CCNGG 8 cut(s) 329, 341, 661, 719, 752, 1246, 1289, 2755
Mva1269I GAATGC 1 cut(s) 846
MvaI CCWGG 6 cut(s) 329, 661, 719, 752, 1289, 2755
MvnI CGCG 4 cut(s) 79, 241, 318, 528
MwoI GCNNNNNNNGC 7 cut(s) 288, 590, 596, 1815, 1847, 2238, 2972
NciI CCSGG 2 cut(s) 341, 1246
NcoI CCATGG 2 cut(s) 909, 1142
NdeI CATATG 1 cut(s) 350
NdeII GATC 9 cut(s) 54, 87, 486, 510, 1984, 2473, 2891, 2933, 3005
NlaIV GGNNCC 7 cut(s) 134, 1126, 1518, 1861, 2096, 2475, 2893
NmeAIII GCCGAG 1 cut(s) 131
NmuCI GTSAC 2 cut(s) 137, 2951
NspI RCATGY 4 cut(s) 790, 1934, 2543, 2581
PaeI GCATGC 1 cut(s) 2543
PaeR7I CTCGAG 1 cut(s) 380
PagI TCATGA 1 cut(s) 2628
PaqCI CACCTGC 1 cut(s) 1301
PceI AGGCCT 1 cut(s) 659
PciSI GCTCTTC 2 cut(s) 1884, 2644
PcsI WCGNNNNNNNCGW 5 cut(s) 153, 315, 603, 687, 705
PctI GAATGC 1 cut(s) 846
PdmI GAANNNNTTC 6 cut(s) 765, 884, 1118, 1164, 2151, 2223
PflFI GACNNNGTC 2 cut(s) 698, 701
PflMI CCANNNNNTGG 1 cut(s) 2484
PpuMI RGGWCCY 1 cut(s) 1084
PshBI ATTAAT 2 cut(s) 993, 1278
Psp124BI GAGCTC 3 cut(s) 111, 976, 1965
Psp5II RGGWCCY 1 cut(s) 1084
Psp6I CCWGG 6 cut(s) 327, 659, 717, 750, 1287, 2753
PspEI GGTNACC 1 cut(s) 1621
PspFI CCCAGC 2 cut(s) 516, 2677
PspGI CCWGG 6 cut(s) 327, 659, 717, 750, 1287, 2753
PspN4I GGNNCC 7 cut(s) 134, 1126, 1518, 1861, 2096, 2475, 2893
PspPI GGNCC 7 cut(s) 180, 721, 748, 1084, 1403, 1703, 2401
PspPPI RGGWCCY 1 cut(s) 1084
PspXI VCTCGAGB 1 cut(s) 380
PstI CTGCAG 1 cut(s) 2977
PstNI CAGNNNCTG 3 cut(s) 1443, 2426, 2591
PsuI RGATCY 6 cut(s) 54, 87, 510, 2473, 2891, 2933
PsyI GACNNNGTC 2 cut(s) 698, 701
PvuII CAGCTG 3 cut(s) 1443, 1496, 2702
RsaI GTAC 8 cut(s) 134, 326, 1698, 2383, 2582, 2660, 2752, 2829
RsaNI GTAC 8 cut(s) 133, 325, 1697, 2382, 2581, 2659, 2751, 2828
SacI GAGCTC 3 cut(s) 111, 976, 1965
SalI GTCGAC 2 cut(s) 491, 2941
SapI GCTCTTC 2 cut(s) 1884, 2644
SaqAI TTAA 9 cut(s) 993, 1278, 1505, 1896, 2207, 2357, 2571, 2655, 2837
Sau3AI GATC 9 cut(s) 54, 87, 486, 510, 1984, 2473, 2891, 2933, 3005
Sau96I GGNCC 7 cut(s) 180, 721, 748, 1084, 1403, 1703, 2401
ScaI AGTACT 2 cut(s) 1698, 2383
ScrFI CCNGG 8 cut(s) 329, 341, 661, 719, 752, 1246, 1289, 2755
SexAI ACCWGGT 1 cut(s) 327
SfaNI GCATC 6 cut(s) 306, 969, 1003, 1417, 2251, 2624
SfcI CTRYAG 2 cut(s) 1737, 2973
Sfr274I CTCGAG 1 cut(s) 380
SinI GGWCC 4 cut(s) 180, 1084, 1403, 2401
SlaI CTCGAG 1 cut(s) 380
SmiI ATTTAAAT 1 cut(s) 2358
SmlI CTYRAG 7 cut(s) 380, 1109, 1259, 1407, 2274, 2314, 2809
SmoI CTYRAG 7 cut(s) 380, 1109, 1259, 1407, 2274, 2314, 2809
SphI GCATGC 1 cut(s) 2543
SseBI AGGCCT 1 cut(s) 659
SspI AATATT 1 cut(s) 2922
SspMI CTAG 4 cut(s) 1088, 1304, 1988, 2258
SstI GAGCTC 3 cut(s) 111, 976, 1965
StuI AGGCCT 1 cut(s) 659
StyD4I CCNGG 8 cut(s) 327, 339, 659, 717, 750, 1244, 1287, 2753
StyI CCWWGG 5 cut(s) 585, 909, 1142, 1303, 1709
SwaI ATTTAAAT 1 cut(s) 2358
TaiI ACGT 5 cut(s) 402, 417, 630, 702, 2495
TatI WGTACW 3 cut(s) 1696, 2381, 2580
Tru1I TTAA 9 cut(s) 993, 1278, 1505, 1896, 2207, 2357, 2571, 2655, 2837
Tru9I TTAA 9 cut(s) 993, 1278, 1505, 1896, 2207, 2357, 2571, 2655, 2837
TscAI CASTG 5 cut(s) 748, 971, 1857, 2596, 2677
TseFI GTSAC 2 cut(s) 137, 2951
TseI GCWGC 9 cut(s) 357, 941, 1043, 1268, 1440, 1493, 1602, 2702, 2972
Tsp45I GTSAC 2 cut(s) 137, 2951
TspGWI ACGGA 5 cut(s) 101, 202, 392, 697, 761
TspRI CASTG 5 cut(s) 748, 971, 1857, 2596, 2677
Tth111I GACNNNGTC 2 cut(s) 698, 701
Van91I CCANNNNNTGG 1 cut(s) 2484
VpaK11BI GGWCC 4 cut(s) 180, 1084, 1403, 2401
VspI ATTAAT 2 cut(s) 993, 1278
XapI RAATTY 1 cut(s) 2198
XbaI TCTAGA 2 cut(s) 1987, 2257
XceI RCATGY 4 cut(s) 790, 1934, 2543, 2581
XcmI CCANNNNNNNNNTGG 2 cut(s) 992, 1713
XhoI CTCGAG 1 cut(s) 380
XmaJI CCTAGG 1 cut(s) 1303
XmiI GTMKAC 4 cut(s) 492, 1651, 1736, 2942
XmnI GAANNNNTTC 6 cut(s) 765, 884, 1118, 1164, 2151, 2223
XspI CTAG 4 cut(s) 1088, 1304, 1988, 2258
ZraI GACGTC 1 cut(s) 700
ZrmI AGTACT 2 cut(s) 1698, 2383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.