AT5G20020

GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003531
Physical Location & Seq
Forward (+)
6762569 .. 6764683
2115 bp
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UTR
Exon/CDS
Intron
AT5G20020.1

Sequence Viewer

Length: 666 bp
ATGGCTCTACCTAACCAACAAACCGTAGATTATCCTAGCTTCAAGCTCGTCATCGTTGGTGATGGAGGCACAGGGAAAACGACCTTTGTGAAGAGACATCTTACTGGGGAGTTTGAGAAGAAGTATGAACCTACTATTGGTGTGGAGGTTCATCCTTTGGATTTCTTCACAAACTGCGGAAAGATCCGTTTTTACTGCTGGGATACTGCTGGACAAGAGAAATTTGGTGGCCTTAGGGATGGATACTACATCCATGGTCAATGTGCGATAATAATGTTTGACGTCACAGCAAGGCTCACATACAAGAATGTTCCGACATGGCATCGTGATCTTTGCAGGGTGTGTGAAAACATCCCGATTGTTCTGTGTGGAAACAAAGTCGATGTGAAGAACAGGCAAGTGAAGGCAAAGCAAGTCACATTCCACAGGAAGAAGAATCTGCAGTACTATGAGATATCAGCAAAGAGCAACTACAACTTCGAGAAGCCTTTCTTGTACCTTGCTAGGAAACTGGCTGGAGACCAGAACCTTCACTTTGTGGAGTCACCAGCTCTTGCTCCACCAGAGGTTCACCTTGACATTGCTGCTCAGCAGCAGAATGAGGCCGATCTTGCAGCTGCTGCAGCTCAGCCTCTCCCCGATGATGATGATGACGCATTTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000054 GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006403 GO:0006405 GO:0006606 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006970 GO:0008104 GO:0008150 GO:0009506 GO:0009628 GO:0009651 GO:0010035 GO:0010038 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017038 GO:0017076 GO:0017111 GO:0019001 GO:0022613 GO:0030054 GO:0030312 GO:0031503 GO:0031974 GO:0031981 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0033036 GO:0033365 GO:0033750 GO:0034504 GO:0034613 GO:0035639 GO:0036094 GO:0042221 GO:0042254 GO:0042886 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046686 GO:0046907 GO:0048046 GO:0050657 GO:0050658 GO:0050896 GO:0051168 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0055044 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0072594 GO:0097159 GO:0097367 GO:1901265 GO:1901363
KEGG Pathways
Metabolic & Signaling
ko03008 ko03013 ko05166 ko05169 map03008 map03013 map05166 map05169
Pfam Domains
Protein Families
Ras

Protein Analysis

221

Amino Acids

25.06

Weight (kDa)

6.38

Isoelectric Point (pI)

28.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 13 - 139 1e-10 ADP-ribosylation factor family
Ras PF00071 15 - 171 1.2e-47 Ras family
Roc PF08477 15 - 128 2.9e-30 Ras of Complex, Roc, domain of DAPkinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 285
AciI CCGC 1 cut(s) 177
AclWI GGATC 1 cut(s) 178
AcsI RAATTY 1 cut(s) 221
AcyI GRCGYC 1 cut(s) 282
AdeI CACNNNGTG 1 cut(s) 538
AfaI GTAC 2 cut(s) 446, 497
AfiI CCNNNNNNNGG 1 cut(s) 137
AgsI TTSAA 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 120, 152
AluBI AGCT 5 cut(s) 39, 46, 551, 617, 626
AluI AGCT 5 cut(s) 39, 46, 551, 617, 626
Alw26I GTCTC 2 cut(s) 88, 513
AlwI GGATC 1 cut(s) 178
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 2 cut(s) 229, 603
ApeKI GCWGC 6 cut(s) 584, 592, 614, 617, 620, 623
ApoI RAATTY 1 cut(s) 221
Asp700I GAANNNNTTC 1 cut(s) 488
AsuHPI GGTGA 3 cut(s) 71, 537, 563
AxyI CCTNAGG 1 cut(s) 233
BbvI GCAGC 6 cut(s) 571, 604, 604, 607, 626, 635
BccI CCATC 2 cut(s) 56, 233
BciVI GTATCC 2 cut(s) 196, 236
BcoDI GTCTC 2 cut(s) 88, 513
BfaI CTAG 2 cut(s) 36, 504
BfmI CTRYAG 2 cut(s) 440, 621
BfuI GTATCC 2 cut(s) 196, 236
BisI GCNGC 6 cut(s) 585, 593, 615, 618, 621, 624
BlpI GCTNAGC 2 cut(s) 588, 627
BlsI GCNGC 6 cut(s) 586, 594, 616, 619, 622, 625
BmcAI AGTACT 1 cut(s) 446
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 1 cut(s) 331
BmuI ACTGGG 1 cut(s) 114
BpmI CTGGAG 1 cut(s) 537
Bpu1102I GCTNAGC 2 cut(s) 588, 627
BsaHI GRCGYC 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 513
BsaJI CCNNGG 1 cut(s) 253
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse1I ACTGG 2 cut(s) 109, 516
Bse21I CCTNAGG 1 cut(s) 233
Bse3DI GCAATG 1 cut(s) 579
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 4 cut(s) 151, 244, 249, 351
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 2 cut(s) 602, 641
BseNI ACTGG 2 cut(s) 109, 516
BseXI GCAGC 6 cut(s) 571, 604, 604, 607, 626, 635
BseYI CCCAGC 1 cut(s) 198
BshFI GGCC 2 cut(s) 231, 605
BslI CCNNNNNNNGG 1 cut(s) 137
BsmAI GTCTC 2 cut(s) 88, 513
BsnI GGCC 2 cut(s) 231, 605
Bso31I GGTCTC 1 cut(s) 513
Bsp143I GATC 3 cut(s) 183, 328, 607
Bsp1720I GCTNAGC 2 cut(s) 588, 627
Bsp19I CCATGG 1 cut(s) 253
BspACI CCGC 1 cut(s) 177
BspANI GGCC 2 cut(s) 231, 605
BspCNI CTCAG 2 cut(s) 601, 640
BspMAI CTGCAG 2 cut(s) 444, 625
BspPI GGATC 1 cut(s) 178
BspTNI GGTCTC 1 cut(s) 513
BsrDI GCAATG 1 cut(s) 579
BsrI ACTGG 2 cut(s) 109, 516
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 3 cut(s) 183, 328, 607
BssNI GRCGYC 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 253
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 86
BstACI GRCGYC 1 cut(s) 282
BstAPI GCANNNNNTGC 1 cut(s) 620
BstDEI CTNAG 3 cut(s) 233, 588, 627
BstDSI CCRYGG 1 cut(s) 253
BstF5I GGATG 4 cut(s) 151, 244, 249, 351
BstKTI GATC 3 cut(s) 186, 331, 610
BstMAI GTCTC 2 cut(s) 88, 513
BstMBI GATC 3 cut(s) 183, 328, 607
BstMWI GCNNNNNNNGC 3 cut(s) 611, 620, 623
BstSFI CTRYAG 2 cut(s) 440, 621
BstV1I GCAGC 6 cut(s) 571, 604, 604, 607, 626, 635
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
Bsu36I CCTNAGG 1 cut(s) 233
BsuI GTATCC 2 cut(s) 196, 236
BsuRI GGCC 2 cut(s) 231, 605
BtgI CCRYGG 1 cut(s) 253
BtsCI GGATG 4 cut(s) 151, 244, 249, 351
CaiI CAGNNNCTG 1 cut(s) 620
Csp6I GTAC 2 cut(s) 445, 496
CviAII CATG 2 cut(s) 254, 318
CviQI GTAC 2 cut(s) 445, 496
DdeI CTNAG 3 cut(s) 233, 588, 627
DpnI GATC 3 cut(s) 185, 330, 609
DpnII GATC 3 cut(s) 183, 328, 607
DraIII CACNNNGTG 1 cut(s) 538
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
Eco130I CCWWGG 1 cut(s) 253
Eco31I GGTCTC 1 cut(s) 513
Eco32I GATATC 1 cut(s) 456
Eco81I CCTNAGG 1 cut(s) 233
EcoRV GATATC 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 253
FaeI CATG 2 cut(s) 257, 321
FaiI YATR 5 cut(s) 126, 255, 301, 319, 450
FalI AAGNNNNNCTT 2 cut(s) 476, 508
FatI CATG 2 cut(s) 253, 317
Fnu4HI GCNGC 6 cut(s) 585, 593, 615, 618, 621, 624
FokI GGATG 4 cut(s) 138, 236, 251, 338
Fsp4HI GCNGC 6 cut(s) 585, 593, 615, 618, 621, 624
FspBI CTAG 2 cut(s) 36, 504
GluI GCNGC 6 cut(s) 585, 593, 615, 618, 621, 624
GsaI CCCAGC 1 cut(s) 202
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 2 cut(s) 231, 605
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 2 cut(s) 257, 321
HinfI GANTC 2 cut(s) 436, 542
HphI GGTGA 3 cut(s) 71, 537, 563
Hpy166II GTNNAC 1 cut(s) 571
Hpy188I TCNGA 1 cut(s) 315
Hpy188III TCNNGA 3 cut(s) 326, 355, 481
Hpy8I GTNNAC 1 cut(s) 571
HpyAV CCTTC 2 cut(s) 397, 539
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 4 cut(s) 336, 442, 614, 623
HpyF10VI GCNNNNNNNGC 3 cut(s) 611, 620, 623
HpyF3I CTNAG 3 cut(s) 233, 588, 627
HpySE526I ACGT 1 cut(s) 282
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 2 cut(s) 257, 321
Kzo9I GATC 3 cut(s) 183, 328, 607
LmnI GCTCC 1 cut(s) 562
Lsp1109I GCAGC 6 cut(s) 571, 604, 604, 607, 626, 635
LweI GCATC 1 cut(s) 331
MaeI CTAG 2 cut(s) 36, 504
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 3 cut(s) 283, 415, 543
MalI GATC 3 cut(s) 185, 330, 609
MboI GATC 3 cut(s) 183, 328, 607
MboII GAAGA 6 cut(s) 103, 130, 157, 400, 442, 445
MflI RGATCY 1 cut(s) 183
MluCI AATT 1 cut(s) 221
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 1 cut(s) 338
MnlI CCTC 5 cut(s) 59, 139, 559, 595, 642
MroXI GAANNNNTTC 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 617
MwoI GCNNNNNNNGC 3 cut(s) 611, 620, 623
NcoI CCATGG 1 cut(s) 253
NdeII GATC 3 cut(s) 183, 328, 607
NlaIII CATG 2 cut(s) 257, 321
NmuCI GTSAC 3 cut(s) 283, 415, 543
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 1 cut(s) 436
PkrI GCNGC 6 cut(s) 586, 594, 616, 619, 622, 625
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
PspFI CCCAGC 1 cut(s) 198
PstI CTGCAG 2 cut(s) 444, 625
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 1 cut(s) 183
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 2 cut(s) 446, 497
RsaNI GTAC 2 cut(s) 445, 496
SatI GCNGC 6 cut(s) 585, 593, 615, 618, 621, 624
Sau3AI GATC 3 cut(s) 183, 328, 607
ScaI AGTACT 1 cut(s) 446
SchI GAGTC 1 cut(s) 551
SfaNI GCATC 1 cut(s) 331
SfcI CTRYAG 2 cut(s) 440, 621
Sse9I AATT 1 cut(s) 221
SsiI CCGC 1 cut(s) 177
SspMI CTAG 2 cut(s) 36, 504
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 1 cut(s) 285
TaqI TCGA 2 cut(s) 381, 480
TasI AATT 1 cut(s) 221
TatI WGTACW 1 cut(s) 444
TfiI GAWTC 1 cut(s) 436
TseFI GTSAC 3 cut(s) 283, 415, 543
TseI GCWGC 6 cut(s) 584, 592, 614, 617, 620, 623
Tsp45I GTSAC 3 cut(s) 283, 415, 543
TspDTI ATGAA 2 cut(s) 140, 141
TspGWI ACGGA 1 cut(s) 176
XapI RAATTY 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 488
XspI CTAG 2 cut(s) 36, 504
ZraI GACGTC 1 cut(s) 283
ZrmI AGTACT 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.