MD15G1120300.v1.1

ribosome localization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003531
Physical Location & Seq
Forward (+)
8653921 .. 8656341
2421 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1120300.v1.1.491

Sequence Viewer

Length: 669 bp
ATGGCTCTTCCGAATCAGCCAACTGTAGATTACCCTAGCTTCAAGCTTGTCATCGTCGGCGATGGTGGAACAGGGAAGACCACTTTCGTGAAACGCCATCTGACCGGAGAGTTTGAAAAGAAATATGAACCAACGATCGGTGTTGAGGTTCATCCGTTGGACTTCTTCACAAATTTTGGGAAGATCAGGTTCTACTCCTGGGACACAGCTGGTCAGGAGAAGTTTGGTGGACTCCGAGATGGTTATTATATTCATGGACAGTGTGCCATCATCATGTTTGATGTTACTGCTCGTTTGACATACAAAAATGTCCCGACATGGCACCGCGATCTCTGCAGGGTTTGTGAGAACATACCTATTGTTCTGTGCGGAAACAAGGTAGATGTGAAAAATAGGCAGGTGAAAGCAAAGCAGGTTACCTTTCACAGGAAGAAGAATCTCCAGTACTATGAGATTTCTGCCAAGAGCAATTACAACTTTGAGAAACCTTTCTTATACCTGGCCAGGAAGCTTGCAGGAAACAATGATCTTCACTTTGTTGAATCTCCTGCTCTTGCTCCTCCGGAGGTTCATATTGACATGGCTGTACAAGCAAAGCATGAACAAGAATTGAAAGATGCAGCTGCACAACCACTTCCTGATGACGATGATGATCTGATTGATTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000054 GO:0000055 GO:0000056 GO:0000060 GO:0000070 GO:0000166 GO:0000226 GO:0000278 GO:0000280 GO:0000287 GO:0000819 GO:0001654 GO:0001673 GO:0001882 GO:0001883 GO:0002177 GO:0003006 GO:0003407 GO:0003674 GO:0003676 GO:0003682 GO:0003712 GO:0003713 GO:0003723 GO:0003824 GO:0003924 GO:0005049 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005814 GO:0005815 GO:0005829 GO:0005856 GO:0005929 GO:0006139 GO:0006163 GO:0006259 GO:0006355 GO:0006403 GO:0006404 GO:0006405 GO:0006409 GO:0006606 GO:0006611 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007399 GO:0007417 GO:0007420 GO:0007423 GO:0008104 GO:0008134 GO:0008150 GO:0008152 GO:0009116 GO:0009117 GO:0009119 GO:0009141 GO:0009144 GO:0009150 GO:0009199 GO:0009205 GO:0009259 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010586 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0012505 GO:0014070 GO:0015031 GO:0015630 GO:0015833 GO:0015931 GO:0016032 GO:0016043 GO:0016070 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017038 GO:0017076 GO:0017111 GO:0019001 GO:0019003 GO:0019058 GO:0019216 GO:0019218 GO:0019219 GO:0019222 GO:0019637 GO:0019693 GO:0019904 GO:0019953 GO:0021537 GO:0021543 GO:0021761 GO:0021766 GO:0022402 GO:0022412 GO:0022414 GO:0022613 GO:0023052 GO:0030029 GO:0030036 GO:0030154 GO:0030496 GO:0030518 GO:0030521 GO:0030522 GO:0030900 GO:0031047 GO:0031074 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031410 GO:0031503 GO:0031514 GO:0031960 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0031982 GO:0032092 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032504 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032870 GO:0032879 GO:0032880 GO:0032991 GO:0033036 GO:0033157 GO:0033365 GO:0033750 GO:0033993 GO:0034504 GO:0034613 GO:0034629 GO:0034641 GO:0034660 GO:0035068 GO:0035194 GO:0035195 GO:0035257 GO:0035258 GO:0035281 GO:0035639 GO:0036094 GO:0036126 GO:0040029 GO:0042221 GO:0042254 GO:0042272 GO:0042278 GO:0042565 GO:0042886 GO:0042995 GO:0043010 GO:0043073 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043393 GO:0043401 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044451 GO:0044464 GO:0044703 GO:0044766 GO:0044877 GO:0045184 GO:0045505 GO:0045540 GO:0045893 GO:0045935 GO:0046039 GO:0046128 GO:0046483 GO:0046794 GO:0046822 GO:0046824 GO:0046825 GO:0046827 GO:0046872 GO:0046890 GO:0046907 GO:0046982 GO:0046983 GO:0048232 GO:0048285 GO:0048468 GO:0048513 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048545 GO:0048609 GO:0048731 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050681 GO:0050789 GO:0050794 GO:0050810 GO:0050896 GO:0051030 GO:0051031 GO:0051049 GO:0051050 GO:0051098 GO:0051099 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051276 GO:0051385 GO:0051427 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051704 GO:0051716 GO:0055037 GO:0055086 GO:0060041 GO:0060255 GO:0060322 GO:0060341 GO:0061015 GO:0061676 GO:0062012 GO:0065007 GO:0065009 GO:0070013 GO:0070201 GO:0070727 GO:0070883 GO:0070887 GO:0071166 GO:0071310 GO:0071383 GO:0071384 GO:0071389 GO:0071396 GO:0071407 GO:0071426 GO:0071428 GO:0071431 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072521 GO:0072594 GO:0075733 GO:0080090 GO:0090087 GO:0090181 GO:0090304 GO:0090316 GO:0097064 GO:0097159 GO:0097223 GO:0097367 GO:0097708 GO:0097729 GO:0098813 GO:0106118 GO:0120025 GO:0140014 GO:0140104 GO:0140110 GO:0140142 GO:1901068 GO:1901135 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901657 GO:1902570 GO:1902579 GO:1902680 GO:1902850 GO:1902930 GO:1903047 GO:1903506 GO:1903508 GO:1903827 GO:1903829 GO:1904951 GO:1990904 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03008 ko03013 ko05166 ko05169 map03008 map03013 map05166 map05169
Pfam Domains
Protein Families
Ras

Protein Analysis

223

Amino Acids

25.31

Weight (kDa)

6.71

Isoelectric Point (pI)

30.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 13 - 139 2.2e-10 ADP-ribosylation factor family
Ras PF00071 15 - 171 3.4e-47 Ras family
Roc PF08477 15 - 128 9.9e-30 Ras of Complex, Roc, domain of DAPkinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 388
Acc36I ACCTGC 2 cut(s) 388, 403
AccB1I GGYRCC 1 cut(s) 321
AccII CGCG 1 cut(s) 327
AccIII TCCGGA 1 cut(s) 562
AciI CCGC 2 cut(s) 325, 369
AcoI YGGCCR 1 cut(s) 501
AcsI RAATTY 1 cut(s) 172
AfaI GTAC 2 cut(s) 446, 588
AfiI CCNNNNNNNGG 2 cut(s) 137, 426
AgsI TTSAA 4 cut(s) 43, 116, 542, 613
AjnI CCWGG 3 cut(s) 197, 498, 503
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 5 cut(s) 39, 46, 209, 511, 623
AluI AGCT 5 cut(s) 39, 46, 209, 511, 623
Aor13HI TCCGGA 1 cut(s) 562
AoxI GGCC 1 cut(s) 501
ApeKI GCWGC 2 cut(s) 620, 623
ApoI RAATTY 1 cut(s) 172
Asp700I GAANNNNTTC 1 cut(s) 488
AsuHPI GGTGA 1 cut(s) 412
BalI TGGCCA 1 cut(s) 503
BanI GGYRCC 1 cut(s) 321
BbsI GAAGAC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 610, 632
BccI CCATC 4 cut(s) 56, 105, 233, 275
BciT130I CCWGG 3 cut(s) 199, 500, 505
BfaI CTAG 1 cut(s) 36
BfmI CTRYAG 2 cut(s) 24, 334
BfuAI ACCTGC 2 cut(s) 388, 403
BisI GCNGC 2 cut(s) 621, 624
BlsI GCNGC 2 cut(s) 622, 625
BmcAI AGTACT 1 cut(s) 446
Bme1390I CCNGG 3 cut(s) 199, 500, 505
BmiI GGNNCC 1 cut(s) 323
BmrFI CCNGG 3 cut(s) 199, 500, 505
BmsI GCATC 1 cut(s) 607
BpiI GAAGAC 1 cut(s) 83
BpmI CTGGAG 1 cut(s) 425
BsaBI GATNNNNATC 1 cut(s) 651
BsaJI CCNNGG 1 cut(s) 198
BsaWI WCCGGW 2 cut(s) 104, 562
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 426
Bse1I ACTGG 1 cut(s) 442
Bse8I GATNNNNATC 1 cut(s) 651
BseAI TCCGGA 1 cut(s) 562
BseBI CCWGG 3 cut(s) 199, 500, 505
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 1 cut(s) 151
BseJI GATNNNNATC 1 cut(s) 651
BseLI CCNNNNNNNGG 2 cut(s) 137, 426
BseNI ACTGG 1 cut(s) 442
BseRI GAGGAG 1 cut(s) 549
BseXI GCAGC 2 cut(s) 610, 632
BsgI GTGCAG 1 cut(s) 609
Bsh1236I CGCG 1 cut(s) 327
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 1 cut(s) 503
BshNI GGYRCC 1 cut(s) 321
BsiEI CGRYCG 1 cut(s) 138
BsiSI CCGG 2 cut(s) 105, 563
BslFI GGGAC 2 cut(s) 215, 296
BslI CCNNNNNNNGG 2 cut(s) 137, 426
BsmFI GGGAC 2 cut(s) 215, 296
BsnI GGCC 1 cut(s) 503
Bsp13I TCCGGA 1 cut(s) 562
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 5 cut(s) 135, 183, 328, 526, 652
BspACI CCGC 2 cut(s) 325, 369
BspANI GGCC 1 cut(s) 503
BspEI TCCGGA 1 cut(s) 562
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 1 cut(s) 323
BspMAI CTGCAG 1 cut(s) 338
BspMI ACCTGC 2 cut(s) 388, 403
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 321
BsrGI TGTACA 1 cut(s) 586
BsrI ACTGG 1 cut(s) 442
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 5 cut(s) 135, 183, 328, 526, 652
Bst2UI CCWGG 3 cut(s) 199, 500, 505
Bst4CI ACNGT 2 cut(s) 25, 261
Bst6I CTCTTC 1 cut(s) 12
BstAUI TGTACA 1 cut(s) 586
BstC8I GCNNGC 1 cut(s) 513
BstEII GGTNACC 1 cut(s) 415
BstENI CCTNNNNNAGG 1 cut(s) 424
BstF5I GGATG 1 cut(s) 151
BstFNI CGCG 1 cut(s) 327
BstKTI GATC 5 cut(s) 138, 186, 331, 529, 655
BstMBI GATC 5 cut(s) 135, 183, 328, 526, 652
BstMCI CGRYCG 1 cut(s) 138
BstMWI GCNNNNNNNGC 2 cut(s) 333, 590
BstNI CCWGG 3 cut(s) 199, 500, 505
BstPI GGTNACC 1 cut(s) 415
BstSCI CCNGG 3 cut(s) 197, 498, 503
BstSFI CTRYAG 2 cut(s) 24, 334
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 2 cut(s) 610, 632
BstV2I GAAGAC 1 cut(s) 83
BsuRI GGCC 1 cut(s) 503
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 266
BveI ACCTGC 2 cut(s) 388, 403
Cac8I GCNNGC 1 cut(s) 513
Csp6I GTAC 2 cut(s) 445, 587
CviAII CATG 5 cut(s) 254, 274, 318, 580, 599
CviJI RGCY 9 cut(s) 5, 19, 39, 46, 209, 503, 511, 584, 623
CviKI_1 RGCY 9 cut(s) 5, 19, 39, 46, 209, 503, 511, 584, 623
CviQI GTAC 2 cut(s) 445, 587
DpnI GATC 5 cut(s) 137, 185, 330, 528, 654
DpnII GATC 5 cut(s) 135, 183, 328, 526, 652
EaeI YGGCCR 1 cut(s) 501
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco91I GGTNACC 1 cut(s) 415
EcoNI CCTNNNNNAGG 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 415
EcoRII CCWGG 3 cut(s) 197, 498, 503
FaeI CATG 5 cut(s) 257, 277, 321, 583, 602
FaqI GGGAC 2 cut(s) 215, 296
FatI CATG 5 cut(s) 253, 273, 317, 579, 598
Fnu4HI GCNGC 2 cut(s) 621, 624
FokI GGATG 1 cut(s) 138
Fsp4HI GCNGC 2 cut(s) 621, 624
FspBI CTAG 1 cut(s) 36
GluI GCNGC 2 cut(s) 621, 624
GsuI CTGGAG 1 cut(s) 425
HaeIII GGCC 1 cut(s) 503
HapII CCGG 2 cut(s) 105, 563
Hin1II CATG 5 cut(s) 257, 277, 321, 583, 602
HindIII AAGCTT 2 cut(s) 44, 509
HinfI GANTC 5 cut(s) 13, 231, 436, 542, 662
HpaII CCGG 2 cut(s) 105, 563
HphI GGTGA 1 cut(s) 412
Hpy166II GTNNAC 1 cut(s) 230
Hpy188I TCNGA 4 cut(s) 12, 102, 236, 657
Hpy188III TCNNGA 6 cut(s) 88, 215, 313, 563, 638, 666
Hpy8I GTNNAC 1 cut(s) 230
Hpy99I CGWCG 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 25, 261
HpyCH4V TGCA 4 cut(s) 336, 515, 620, 626
HpyF10VI GCNNNNNNNGC 2 cut(s) 333, 590
Hsp92II CATG 5 cut(s) 257, 277, 321, 583, 602
Kpn2I TCCGGA 1 cut(s) 562
Kzo9I GATC 5 cut(s) 135, 183, 328, 526, 652
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 562
Lsp1109I GCAGC 2 cut(s) 610, 632
LweI GCATC 1 cut(s) 607
MaeI CTAG 1 cut(s) 36
MaeIII GTNAC 2 cut(s) 283, 415
MalI GATC 5 cut(s) 137, 185, 330, 528, 654
MboI GATC 5 cut(s) 135, 183, 328, 526, 652
MboII GAAGA 6 cut(s) 88, 157, 193, 442, 445, 521
MlsI TGGCCA 1 cut(s) 503
MluCI AATT 3 cut(s) 172, 469, 608
MluNI TGGCCA 1 cut(s) 503
MlyI GAGTC 1 cut(s) 225
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 3 cut(s) 139, 559, 570
Mox20I TGGCCA 1 cut(s) 503
MroI TCCGGA 1 cut(s) 562
MroXI GAANNNNTTC 1 cut(s) 488
MscI TGGCCA 1 cut(s) 503
MslI CAYNNNNRTG 2 cut(s) 86, 272
Msp20I TGGCCA 1 cut(s) 503
MspA1I CMGCKG 2 cut(s) 209, 623
MspI CCGG 2 cut(s) 105, 563
MspR9I CCNGG 3 cut(s) 199, 500, 505
MvaI CCWGG 3 cut(s) 199, 500, 505
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 2 cut(s) 333, 590
NdeII GATC 5 cut(s) 135, 183, 328, 526, 652
NlaIII CATG 5 cut(s) 257, 277, 321, 583, 602
NlaIV GGNNCC 1 cut(s) 323
OliI CACNNNNGTG 1 cut(s) 86
PaqCI CACCTGC 1 cut(s) 388
PciSI GCTCTTC 1 cut(s) 12
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 4 cut(s) 13, 436, 542, 662
PkrI GCNGC 2 cut(s) 622, 625
Ple19I CGATCG 1 cut(s) 138
PleI GAGTC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 225
Psp6I CCWGG 3 cut(s) 197, 498, 503
PspEI GGTNACC 1 cut(s) 415
PspGI CCWGG 3 cut(s) 197, 498, 503
PspN4I GGNNCC 1 cut(s) 323
PstI CTGCAG 1 cut(s) 338
PvuI CGATCG 1 cut(s) 138
PvuII CAGCTG 2 cut(s) 209, 623
RsaI GTAC 2 cut(s) 446, 588
RsaNI GTAC 2 cut(s) 445, 587
RseI CAYNNNNRTG 2 cut(s) 86, 272
SapI GCTCTTC 1 cut(s) 12
SatI GCNGC 2 cut(s) 621, 624
Sau3AI GATC 5 cut(s) 135, 183, 328, 526, 652
ScaI AGTACT 1 cut(s) 446
SchI GAGTC 1 cut(s) 225
ScrFI CCNGG 3 cut(s) 199, 500, 505
SfaNI GCATC 1 cut(s) 607
SfcI CTRYAG 2 cut(s) 24, 334
SmiMI CAYNNNNRTG 2 cut(s) 86, 272
Sse9I AATT 3 cut(s) 172, 469, 608
SsiI CCGC 2 cut(s) 325, 369
SspMI CTAG 1 cut(s) 36
StyD4I CCNGG 3 cut(s) 197, 498, 503
TaaI ACNGT 2 cut(s) 25, 261
TasI AATT 3 cut(s) 172, 469, 608
TatI WGTACW 2 cut(s) 444, 586
TfiI GAWTC 4 cut(s) 13, 436, 542, 662
TscAI CASTG 1 cut(s) 266
TseI GCWGC 2 cut(s) 620, 623
TspDTI ATGAA 5 cut(s) 140, 141, 242, 560, 615
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 1 cut(s) 266
XagI CCTNNNNNAGG 1 cut(s) 424
XapI RAATTY 1 cut(s) 172
XmnI GAANNNNTTC 1 cut(s) 488
XspI CTAG 1 cut(s) 36
ZrmI AGTACT 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.