AT5G22330

Belongs to the RuvB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002367
Physical Location & Seq
Reverse (-)
7390796 .. 7394280
3485 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22330.1

Sequence Viewer

Length: 1377 bp
ATGGAGAAAGTAAAGATTGAAGAAATTCAGTCCACCGCTAAGAAACAACGGATTGCTACTCACACCCATATCAAAGGCCTTGGCCTCGAGCCAACTGGTATCCCTATAAAATTGGCAGCTGGATTTGTTGGTCAACTTGAGGCTAGAGAGGCAGCTGGTCTTGTAGTTGACATGATTAAGCAGAAGAAAATGGCGGGCAAGGCTCTTTTGCTTGCTGGACCTCCTGGAACTGGGAAAACAGCTTTGGCTCTTGGAATCTCTCAAGAGCTGGGAAGCAAGGTTCCATTCTGTCCAATGGTTGGATCTGAGGTTTACTCATCAGAGGTTAAGAAAACAGAGGTTCTCATGGAGAATTTTAGACGTGCCATTGGTCTACGTATCAAGGAAACCAAAGAAGTCTATGAAGGGGAGGTCACCGAGCTGTCACCAGAAGAAACTGAAAGCCTCACTGGAGGTTATGGTAAAAGCATCAGCCATGTTGTAATTACACTCAAGACAGTCAAAGGAACCAAACATCTGAAATTGGATCCCACTATCTATGATGCCTTGATTAAGGAAAAGGTAGCTGTAGGAGATGTAATCTATATCGAAGCAAACAGTGGAGCTGTCAAACGGGTAGGTAGAAGTGATGCTTTTGCCACTGAATTTGATCTGGAAGCAGAAGAATATGTTCCACTTCCCAAAGGAGAGGTCCACAAAAAGAAAGAGATAGTGCAGGATGTCACACTCCAAGATCTGGATGCAGCAAATGCTCGACCTCAAGGTGGCCAGGATATACTTTCTTTGATGGGCCAAATGATGAAACCGCGGAAGACTGAGATCACTGATAAGCTTCGGCAAGAAATTAACAAGGTTGTGAACCGATATATAGATGAAGGTGTGGCAGAGCTTGTTCCAGGAGTTCTATTTATTGATGAGGTTCATATGCTTGATATGGAGTGCTTCTCATACTTGAACCGTGCTCTTGAGAGCTCATTATCTCCGATAGTGATATTTGCAACAAATAGAGGTGTTTGCAACGTAAGAGGGACTGATATGCCCAGCCCCCATGGAGTCCCTATTGATCTATTAGATCGATTGGTTATCATCCGGACTCAAATCTATGATCCCTCTGAAATGATCCAGATTATAGCCATTCGTGCGCAAGTTGAAGAATTAACCGTGGATGAAGAATGCTTGGTTCTACTTGGGGAGATTGGGCAAAGAACTTCACTAAGGCACGCTGTGCAGCTTCTGTCTCCTGCCAGCATTGTAGCGAAAATGAATGGCCGTGACAATATTTGCAAGGCTGATATAGAGGAAGTAACATCACTCTACTTGGATGCTAAATCTTCAGCAAAGCTTTTGCATGAGCAACAAGAAAAATACATCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000491 GO:0000492 GO:0000785 GO:0000790 GO:0000812 GO:0002682 GO:0002831 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009888 GO:0009889 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010604 GO:0010628 GO:0010755 GO:0010756 GO:0010941 GO:0010954 GO:0016043 GO:0016363 GO:0016462 GO:0016569 GO:0016570 GO:0016573 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0030162 GO:0031011 GO:0031248 GO:0031323 GO:0031325 GO:0031326 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032270 GO:0032392 GO:0032502 GO:0032508 GO:0032991 GO:0033202 GO:0034399 GO:0034622 GO:0034708 GO:0035097 GO:0035267 GO:0036211 GO:0042623 GO:0042981 GO:0043067 GO:0043138 GO:0043140 GO:0043170 GO:0043189 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043900 GO:0043933 GO:0043967 GO:0043968 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044665 GO:0045088 GO:0045862 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048856 GO:0050776 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070035 GO:0070603 GO:0070613 GO:0071103 GO:0071339 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0080134 GO:0097255 GO:0097346 GO:0140097 GO:1900150 GO:1901564 GO:1902493 GO:1902494 GO:1902562 GO:1903317 GO:1903319 GO:1903506 GO:1904949 GO:1990234 GO:2000072 GO:2000112 GO:2000269 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
TIP49

Protein Analysis

458

Amino Acids

50.32

Weight (kDa)

5.67

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIP49 PF06068 17 - 369 4.6e-165 TIP49 P-loop domain
RuvB_N PF05496 40 - 94 6.8e-07 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 69 - 118 1.2e-06 ATPase family associated with various cellular activities (AAA)
TIP49_C PF17856 376 - 441 3.5e-20 TIP49 AAA-lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1143
AccB7I CCANNNNNTGG 2 cut(s) 299, 736
AccI GTMKAC 1 cut(s) 373
AccII CGCG 1 cut(s) 808
AccIII TCCGGA 1 cut(s) 1089
AciI CCGC 4 cut(s) 36, 194, 806, 808
AclWI GGATC 5 cut(s) 310, 521, 534, 1100, 1114
AcoI YGGCCR 2 cut(s) 766, 1267
AcsI RAATTY 3 cut(s) 24, 352, 644
AcuI CTGAAG 1 cut(s) 1317
AdeI CACNNNGTG 1 cut(s) 1225
AfiI CCNNNNNNNGG 4 cut(s) 230, 299, 736, 764
AgsI TTSAA 3 cut(s) 20, 955, 1151
AjiI CACGTC 1 cut(s) 362
AjnI CCWGG 3 cut(s) 223, 768, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AloI GAACNNNNNNTCC 2 cut(s) 264, 296
Alw21I GWGCWC 2 cut(s) 964, 974
Alw26I GTCTC 1 cut(s) 1242
AlwI GGATC 5 cut(s) 310, 521, 534, 1100, 1114
AlwNI CAGNNNCTG 1 cut(s) 1234
Ama87I CYCGRG 1 cut(s) 86
Aor13HI TCCGGA 1 cut(s) 1089
AoxI GGCC 5 cut(s) 76, 82, 766, 790, 1267
ApeKI GCWGC 4 cut(s) 116, 152, 743, 1228
ApoI RAATTY 3 cut(s) 24, 352, 644
ArsI GACNNNNNNTTYG 2 cut(s) 675, 707
Asp700I GAANNNNTTC 2 cut(s) 24, 669
AspLEI GCGC 1 cut(s) 1144
AspS9I GGNCC 3 cut(s) 218, 691, 790
AsuHPI GGTGA 2 cut(s) 406, 417
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 2 cut(s) 218, 691
BalI TGGCCA 1 cut(s) 768
BamHI GGATCC 1 cut(s) 526
BanII GRGCYC 1 cut(s) 974
BbsI GAAGAC 1 cut(s) 818
Bbv12I GWGCWC 2 cut(s) 964, 974
BbvI GCAGC 4 cut(s) 128, 164, 755, 1240
BccI CCATC 1 cut(s) 781
BceAI ACGGC 1 cut(s) 1254
BciT130I CCWGG 3 cut(s) 225, 770, 897
BciVI GTATCC 1 cut(s) 110
BcoDI GTCTC 1 cut(s) 1242
BfaI CTAG 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 567
BfuI GTATCC 1 cut(s) 110
BglII AGATCT 1 cut(s) 733
BisI GCNGC 4 cut(s) 117, 153, 744, 1229
BlsI GCNGC 4 cut(s) 118, 154, 745, 1230
Bme1390I CCNGG 3 cut(s) 225, 770, 897
Bme18I GGWCC 2 cut(s) 218, 691
BmeT110I CYCGRG 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 362
BmgT120I GGNCC 3 cut(s) 218, 691, 790
BmiI GGNNCC 3 cut(s) 282, 508, 528
BmrFI CCNGG 3 cut(s) 225, 770, 897
BmrI ACTGGG 1 cut(s) 240
BmsI GCATC 5 cut(s) 477, 532, 619, 730, 1312
BmuI ACTGGG 1 cut(s) 240
BpiI GAAGAC 1 cut(s) 818
BplI GAGNNNNNCTC 4 cut(s) 299, 331, 929, 961
BpmI CTGGAG 1 cut(s) 471
BpuEI CTTGAG 5 cut(s) 158, 246, 476, 744, 986
Bsa29I ATCGAT 1 cut(s) 1075
BsaAI YACGTR 1 cut(s) 377
BsaJI CCNNGG 4 cut(s) 79, 806, 1048, 1161
BsaWI WCCGGW 1 cut(s) 1089
Bsc4I CCNNNNNNNGG 4 cut(s) 230, 299, 736, 764
Bse1I ACTGG 3 cut(s) 100, 235, 454
BseAI TCCGGA 1 cut(s) 1089
BseBI CCWGG 3 cut(s) 225, 770, 897
BseCI ATCGAT 1 cut(s) 1075
BseDI CCNNGG 4 cut(s) 79, 806, 1048, 1161
BseGI GGATG 5 cut(s) 724, 745, 1086, 1171, 1327
BseLI CCNNNNNNNGG 4 cut(s) 230, 299, 736, 764
BseMII CTCAG 2 cut(s) 297, 807
BseNI ACTGG 3 cut(s) 100, 235, 454
BseXI GCAGC 4 cut(s) 128, 164, 755, 1240
BseYI CCCAGC 2 cut(s) 268, 1040
BsgI GTGCAG 2 cut(s) 734, 1247
Bsh1236I CGCG 1 cut(s) 808
BshFI GGCC 5 cut(s) 78, 84, 768, 792, 1269
BshVI ATCGAT 1 cut(s) 1075
BsiHKAI GWGCWC 2 cut(s) 964, 974
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 1 cut(s) 1090
BslFI GGGAC 2 cut(s) 1040, 1042
BslI CCNNNNNNNGG 4 cut(s) 230, 299, 736, 764
BsmAI GTCTC 1 cut(s) 1242
BsmFI GGGAC 2 cut(s) 1040, 1042
BsmI GAATGC 1 cut(s) 1178
BsnI GGCC 5 cut(s) 78, 84, 768, 792, 1269
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 2 cut(s) 964, 974
Bsp13I TCCGGA 1 cut(s) 1089
Bsp143I GATC 9 cut(s) 302, 526, 649, 733, 819, 1063, 1072, 1105, 1119
Bsp19I CCATGG 1 cut(s) 1048
BspACI CCGC 4 cut(s) 36, 194, 806, 808
BspANI GGCC 5 cut(s) 78, 84, 768, 792, 1269
BspCNI CTCAG 2 cut(s) 298, 808
BspDI ATCGAT 1 cut(s) 1075
BspEI TCCGGA 1 cut(s) 1089
BspFNI CGCG 1 cut(s) 808
BspHI TCATGA 1 cut(s) 1373
BspLI GGNNCC 3 cut(s) 282, 508, 528
BspPI GGATC 5 cut(s) 310, 521, 534, 1100, 1114
BsrI ACTGG 3 cut(s) 100, 235, 454
BssECI CCNNGG 4 cut(s) 79, 806, 1048, 1161
BssMI GATC 9 cut(s) 302, 526, 649, 733, 819, 1063, 1072, 1105, 1119
BssT1I CCWWGG 2 cut(s) 79, 1048
Bst2UI CCWGG 3 cut(s) 225, 770, 897
Bst4CI ACNGT 4 cut(s) 499, 599, 959, 1162
BstAPI GCANNNNNTGC 2 cut(s) 749, 1225
BstBAI YACGTR 1 cut(s) 377
BstC8I GCNNGC 4 cut(s) 196, 213, 1221, 1246
BstDEI CTNAG 4 cut(s) 39, 306, 816, 1214
BstDSI CCRYGG 3 cut(s) 806, 1048, 1161
BstEII GGTNACC 1 cut(s) 412
BstF5I GGATG 5 cut(s) 724, 745, 1086, 1171, 1327
BstFNI CGCG 1 cut(s) 808
BstHHI GCGC 1 cut(s) 1144
BstKTI GATC 9 cut(s) 305, 529, 652, 736, 822, 1066, 1075, 1108, 1122
BstMAI GTCTC 1 cut(s) 1242
BstMBI GATC 9 cut(s) 302, 526, 649, 733, 819, 1063, 1072, 1105, 1119
BstMWI GCNNNNNNNGC 5 cut(s) 149, 200, 749, 1139, 1225
BstNI CCWGG 3 cut(s) 225, 770, 897
BstPI GGTNACC 1 cut(s) 412
BstSCI CCNGG 3 cut(s) 223, 768, 895
BstSFI CTRYAG 1 cut(s) 567
BstSNI TACGTA 1 cut(s) 377
BstUI CGCG 1 cut(s) 808
BstV1I GCAGC 4 cut(s) 128, 164, 755, 1240
BstV2I GAAGAC 1 cut(s) 818
BstX2I RGATCY 3 cut(s) 302, 526, 733
BstYI RGATCY 3 cut(s) 302, 526, 733
Bsu15I ATCGAT 1 cut(s) 1075
BsuI GTATCC 1 cut(s) 110
BsuRI GGCC 5 cut(s) 78, 84, 768, 792, 1269
BsuTUI ATCGAT 1 cut(s) 1075
BtgI CCRYGG 3 cut(s) 806, 1048, 1161
BtrI CACGTC 1 cut(s) 362
BtsCI GGATG 5 cut(s) 724, 745, 1086, 1171, 1327
BtsIMutI CAGTG 4 cut(s) 447, 604, 639, 822
Cac8I GCNNGC 4 cut(s) 196, 213, 1221, 1246
CaiI CAGNNNCTG 1 cut(s) 1234
CciI TCATGA 1 cut(s) 1373
CfoI GCGC 1 cut(s) 1144
Cfr13I GGNCC 3 cut(s) 218, 691, 790
Cfr42I CCGCGG 1 cut(s) 809
ClaI ATCGAT 1 cut(s) 1075
CspCI CAANNNNNGTGG 2 cut(s) 628, 663
CviAII CATG 6 cut(s) 172, 346, 476, 1049, 1349, 1374
DdeI CTNAG 4 cut(s) 39, 306, 816, 1214
DpnI GATC 9 cut(s) 304, 528, 651, 735, 821, 1065, 1074, 1107, 1121
DpnII GATC 9 cut(s) 302, 526, 649, 733, 819, 1063, 1072, 1105, 1119
DraIII CACNNNGTG 1 cut(s) 1225
EaeI YGGCCR 2 cut(s) 766, 1267
Ecl136II GAGCTC 1 cut(s) 972
Eco105I TACGTA 1 cut(s) 377
Eco130I CCWWGG 2 cut(s) 79, 1048
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 974
Eco47I GGWCC 2 cut(s) 218, 691
Eco53kI GAGCTC 1 cut(s) 972
Eco57I CTGAAG 1 cut(s) 1317
Eco88I CYCGRG 1 cut(s) 86
Eco91I GGTNACC 1 cut(s) 412
EcoICRI GAGCTC 1 cut(s) 972
EcoO65I GGTNACC 1 cut(s) 412
EcoRII CCWGG 3 cut(s) 223, 768, 895
EcoT14I CCWWGG 2 cut(s) 79, 1048
EcoT38I GRGCYC 1 cut(s) 974
ErhI CCWWGG 2 cut(s) 79, 1048
FaeI CATG 6 cut(s) 175, 349, 479, 1052, 1352, 1377
FalI AAGNNNNNCTT 2 cut(s) 616, 648
FaqI GGGAC 2 cut(s) 1040, 1042
FatI CATG 6 cut(s) 171, 345, 475, 1048, 1348, 1373
FauI CCCGC 1 cut(s) 187
FauNDI CATATG 1 cut(s) 924
FblI GTMKAC 1 cut(s) 373
Fnu4HI GCNGC 4 cut(s) 117, 153, 744, 1229
FokI GGATG 5 cut(s) 731, 752, 1073, 1178, 1334
FriOI GRGCYC 1 cut(s) 974
Fsp4HI GCNGC 4 cut(s) 117, 153, 744, 1229
FspBI CTAG 1 cut(s) 144
FspI TGCGCA 1 cut(s) 1143
GlaI GCGC 1 cut(s) 1143
GluI GCNGC 4 cut(s) 117, 153, 744, 1229
GsaI CCCAGC 2 cut(s) 272, 1044
GsuI CTGGAG 1 cut(s) 471
HaeIII GGCC 5 cut(s) 78, 84, 768, 792, 1269
HapII CCGG 1 cut(s) 1090
HhaI GCGC 1 cut(s) 1144
Hin1II CATG 6 cut(s) 175, 349, 479, 1052, 1352, 1377
Hin6I GCGC 1 cut(s) 1142
HinP1I GCGC 1 cut(s) 1142
HincII GTYRAC 2 cut(s) 134, 169
HindII GTYRAC 2 cut(s) 134, 169
HindIII AAGCTT 2 cut(s) 830, 1340
HinfI GANTC 3 cut(s) 255, 1053, 1093
HpaII CCGG 1 cut(s) 1090
HphI GGTGA 2 cut(s) 406, 417
Hpy166II GTNNAC 7 cut(s) 33, 134, 169, 313, 374, 694, 859
Hpy188I TCNGA 5 cut(s) 307, 322, 519, 984, 1114
Hpy188III TCNNGA 8 cut(s) 263, 493, 653, 737, 965, 1090, 1123, 1374
Hpy8I GTNNAC 7 cut(s) 33, 134, 169, 313, 374, 694, 859
HpyAV CCTTC 2 cut(s) 398, 869
HpyCH4III ACNGT 4 cut(s) 499, 599, 959, 1162
HpyCH4IV ACGT 3 cut(s) 361, 376, 1020
HpyCH4V TGCA 7 cut(s) 715, 743, 998, 1017, 1228, 1284, 1348
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 200, 749, 1139, 1225
HpyF3I CTNAG 4 cut(s) 39, 306, 816, 1214
HpySE526I ACGT 3 cut(s) 361, 376, 1020
Hsp92II CATG 6 cut(s) 175, 349, 479, 1052, 1352, 1377
HspAI GCGC 1 cut(s) 1142
Kpn2I TCCGGA 1 cut(s) 1089
KspI CCGCGG 1 cut(s) 809
Kzo9I GATC 9 cut(s) 302, 526, 649, 733, 819, 1063, 1072, 1105, 1119
LmnI GCTCC 1 cut(s) 602
Lsp1109I GCAGC 4 cut(s) 128, 164, 755, 1240
LweI GCATC 5 cut(s) 477, 532, 619, 730, 1312
MaeI CTAG 1 cut(s) 144
MaeII ACGT 3 cut(s) 361, 376, 1020
MaeIII GTNAC 5 cut(s) 412, 423, 721, 1271, 1303
MalI GATC 9 cut(s) 304, 528, 651, 735, 821, 1065, 1074, 1107, 1121
MboI GATC 9 cut(s) 302, 526, 649, 733, 819, 1063, 1072, 1105, 1119
MboII GAAGA 8 cut(s) 32, 196, 443, 674, 823, 1163, 1181, 1323
MflI RGATCY 3 cut(s) 302, 526, 733
MhlI GDGCHC 2 cut(s) 964, 974
MlsI TGGCCA 1 cut(s) 768
MluCI AATT 8 cut(s) 24, 110, 352, 483, 521, 644, 843, 1154
MluNI TGGCCA 1 cut(s) 768
MlyI GAGTC 2 cut(s) 1062, 1087
MmeI TCCRAC 1 cut(s) 280
Mox20I TGGCCA 1 cut(s) 768
MroI TCCGGA 1 cut(s) 1089
MroXI GAANNNNTTC 2 cut(s) 24, 669
MscI TGGCCA 1 cut(s) 768
MseI TTAA 5 cut(s) 177, 327, 552, 846, 1157
Msp20I TGGCCA 1 cut(s) 768
MspA1I CMGCKG 3 cut(s) 119, 155, 808
MspI CCGG 1 cut(s) 1090
MspR9I CCNGG 3 cut(s) 225, 770, 897
Mva1269I GAATGC 1 cut(s) 1178
MvaI CCWGG 3 cut(s) 225, 770, 897
MvnI CGCG 1 cut(s) 808
MwoI GCNNNNNNNGC 5 cut(s) 149, 200, 749, 1139, 1225
NcoI CCATGG 1 cut(s) 1048
NdeI CATATG 1 cut(s) 924
NdeII GATC 9 cut(s) 302, 526, 649, 733, 819, 1063, 1072, 1105, 1119
NlaIII CATG 6 cut(s) 175, 349, 479, 1052, 1352, 1377
NlaIV GGNNCC 3 cut(s) 282, 508, 528
NmuCI GTSAC 4 cut(s) 412, 423, 721, 1271
NsbI TGCGCA 1 cut(s) 1143
PaeR7I CTCGAG 1 cut(s) 86
PagI TCATGA 1 cut(s) 1373
PceI AGGCCT 1 cut(s) 78
PctI GAATGC 1 cut(s) 1178
PdmI GAANNNNTTC 2 cut(s) 24, 669
PfeI GAWTC 1 cut(s) 255
PflMI CCANNNNNTGG 2 cut(s) 299, 736
PfoI TCCNGGA 2 cut(s) 223, 895
PkrI GCNGC 4 cut(s) 118, 154, 745, 1230
PleI GAGTC 2 cut(s) 1061, 1087
PpsI GAGTC 2 cut(s) 1061, 1087
Ppu21I YACGTR 1 cut(s) 377
Psp124BI GAGCTC 1 cut(s) 974
Psp6I CCWGG 3 cut(s) 223, 768, 895
PspEI GGTNACC 1 cut(s) 412
PspFI CCCAGC 2 cut(s) 268, 1040
PspGI CCWGG 3 cut(s) 223, 768, 895
PspN4I GGNNCC 3 cut(s) 282, 508, 528
PspPI GGNCC 3 cut(s) 218, 691, 790
PspXI VCTCGAGB 1 cut(s) 86
PstNI CAGNNNCTG 1 cut(s) 1234
PsuI RGATCY 3 cut(s) 302, 526, 733
PvuII CAGCTG 2 cut(s) 119, 155
SacI GAGCTC 1 cut(s) 974
SacII CCGCGG 1 cut(s) 809
SaqAI TTAA 5 cut(s) 177, 327, 552, 846, 1157
SatI GCNGC 4 cut(s) 117, 153, 744, 1229
Sau3AI GATC 9 cut(s) 302, 526, 649, 733, 819, 1063, 1072, 1105, 1119
Sau96I GGNCC 3 cut(s) 218, 691, 790
SchI GAGTC 2 cut(s) 1062, 1087
ScrFI CCNGG 3 cut(s) 225, 770, 897
SduI GDGCHC 2 cut(s) 964, 974
SfaNI GCATC 5 cut(s) 477, 532, 619, 730, 1312
SfcI CTRYAG 1 cut(s) 567
Sfr274I CTCGAG 1 cut(s) 86
Sfr303I CCGCGG 1 cut(s) 809
SgrBI CCGCGG 1 cut(s) 809
SinI GGWCC 2 cut(s) 218, 691
SlaI CTCGAG 1 cut(s) 86
SmlI CTYRAG 6 cut(s) 86, 137, 261, 491, 759, 965
SmoI CTYRAG 6 cut(s) 86, 137, 261, 491, 759, 965
SnaBI TACGTA 1 cut(s) 377
Sse9I AATT 8 cut(s) 24, 110, 352, 483, 521, 644, 843, 1154
SseBI AGGCCT 1 cut(s) 78
SsiI CCGC 4 cut(s) 36, 194, 806, 808
SspI AATATT 1 cut(s) 1279
SspMI CTAG 1 cut(s) 144
SstI GAGCTC 1 cut(s) 974
StuI AGGCCT 1 cut(s) 78
StyD4I CCNGG 3 cut(s) 223, 768, 895
StyI CCWWGG 2 cut(s) 79, 1048
TaaI ACNGT 4 cut(s) 499, 599, 959, 1162
TaiI ACGT 3 cut(s) 364, 379, 1023
TaqI TCGA 4 cut(s) 87, 588, 754, 1075
TasI AATT 8 cut(s) 24, 110, 352, 483, 521, 644, 843, 1154
TfiI GAWTC 1 cut(s) 255
Tru1I TTAA 5 cut(s) 177, 327, 552, 846, 1157
Tru9I TTAA 5 cut(s) 177, 327, 552, 846, 1157
TscAI CASTG 4 cut(s) 454, 604, 646, 829
TseFI GTSAC 4 cut(s) 412, 423, 721, 1271
TseI GCWGC 4 cut(s) 116, 152, 743, 1228
Tsp45I GTSAC 4 cut(s) 412, 423, 721, 1271
TspDTI ATGAA 6 cut(s) 417, 815, 888, 911, 1182, 1277
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 4 cut(s) 454, 604, 646, 829
Van91I CCANNNNNTGG 2 cut(s) 299, 736
VpaK11BI GGWCC 2 cut(s) 218, 691
XapI RAATTY 3 cut(s) 24, 352, 644
XhoI CTCGAG 1 cut(s) 86
XmiI GTMKAC 1 cut(s) 373
XmnI GAANNNNTTC 2 cut(s) 24, 669
XspI CTAG 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.