FvH4_3g34140

Belongs to the RuvB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002367
Physical Location & Seq
Forward (+)
29523566 .. 29528631
5066 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34140.t1

Sequence Viewer

Length: 1377 bp
ATGGATAAGGTGAAAATAGAAGAGGTTCAGTCCACTACCAAGAAGCAACGCGTAGCTACTCACACCCACATTAAAGGCCTTGGTCTTGAGGCCAATGGAAGAGCAGCACCTTGGGCTGCTGGCTTTGTGGGTCAGGGGGAGGCAAGAGAAGCTGCTGGTCTTGTTGTCGATATGATACGGCAGAAGAAGTTGGCTGGTAAGGCTCTTCTAATGGCTGGACCTCCTGGAACTGGAAAGACAGCACTGGCTCTTGGAATATCCCAGGAGCTTGGGAGTAAGGTTCCATTCTGCCCAATGGTTGGGTCTGAAGTATATTCATCAGAAGTTAAGAAAACAGAGGTTTTAATGGAAAATTTTAGACGGGCTATTGGTCTACGTATCAAGGAAAACAAAGAGGTCTATGAAGGAGAGGTGACAGAACTAACACCAGAAGAAACGGAGAGTGTGACGGGTGGCTATGGTAAAAGTATCAGCCATGTAATCATTGGATTAAAAACTGTCAAAGGAACCAAGCAATTGAAGTTGGACCCTACTATTTATGACGCATTGGTTAAGGAAAAGGTTGCTGTTGGTGATGTTATATACATTGAAGCAAACAGTGGGGCAGTAAAAAGGGTAGGCAGAAGTGATGCATTTGCTACGGAATTCGATCTTGAAGCAGAAGAGTATGTTCCACTTCCGAAAGGAGAGGTTCACAAAAAGAAGGAGATTGTGCAGGATGTAACTCTGCACGATCTAGATGCTGCGAATGCACGACCTCAAGGTGGGCAAGACATATTATCTCTAATGGGTCAGATGATGAAACCAAGAAAAACAGAAATTACGGACAAGTTGCGACAAGAAATAAACAAGGTTGTTAACCGGTATATTGATGAAGGTGTAGCGGAGCTTGTTCCTGGAGTTCTGTTCATTGATGAGGTACATATGCTGGATATGGAGTGCTTCTCGTACTTGAATCGTGCTTTAGAGAGCTCGTTATCTCCAATAGTAATATTTGCCACAAATAGGGGAATTTGCAACGTGAGAGGGACTGATATGGCTAGTCCTCATGGAATACCTGTTGACTTGTTGGACCGGTTGGTGATTATCCGAACACAGACATATGGTCCTGAAGAGATGATAAAGATATTAGCAACCCGCGCACAGGTGGAGGAACTGGTTGTAGATGAGGAGAGTTTGGCTTACCTTGGGGAGATAGGACAGCGAACGTCTTTAAGGCATGCTGTTCAGCTCTTATCCCCTGCAAGCATTATGGCAAAAATGAATGGCAGAGATAATATCTGCAAGGAGGATTTGGAAGAAGTAGCTACCCTATATCTGGATGCAAAGTCTTCAGCAAAGGTTCTTCAAGAGCAGCAGGAAAAATACATTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000491 GO:0000492 GO:0000785 GO:0000790 GO:0000812 GO:0002682 GO:0002831 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009888 GO:0009889 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010604 GO:0010628 GO:0010755 GO:0010756 GO:0010941 GO:0010954 GO:0016043 GO:0016363 GO:0016462 GO:0016569 GO:0016570 GO:0016573 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0030162 GO:0031011 GO:0031248 GO:0031323 GO:0031325 GO:0031326 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032270 GO:0032392 GO:0032502 GO:0032508 GO:0032991 GO:0033202 GO:0034399 GO:0034622 GO:0034708 GO:0035097 GO:0035267 GO:0036211 GO:0042623 GO:0042981 GO:0043067 GO:0043138 GO:0043140 GO:0043170 GO:0043189 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043900 GO:0043933 GO:0043967 GO:0043968 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044665 GO:0045088 GO:0045862 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048856 GO:0050776 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070035 GO:0070603 GO:0070613 GO:0071103 GO:0071339 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0080134 GO:0097255 GO:0097346 GO:0140097 GO:1900150 GO:1901564 GO:1902493 GO:1902494 GO:1902562 GO:1903317 GO:1903319 GO:1903506 GO:1904949 GO:1990234 GO:2000072 GO:2000112 GO:2000269 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
TIP49

Protein Analysis

459

Amino Acids

50.26

Weight (kDa)

5.62

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIP49 PF06068 17 - 369 1.1e-166 TIP49 P-loop domain
RuvB_N PF05496 41 - 94 2.1e-06 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 69 - 118 2.9e-06 ATPase family associated with various cellular activities (AAA)
TIP49_C PF17856 376 - 441 2.3e-18 TIP49 AAA-lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 299
AccI GTMKAC 1 cut(s) 373
AccII CGCG 2 cut(s) 51, 1140
AciI CCGC 2 cut(s) 884, 1138
AcsI RAATTY 3 cut(s) 352, 644, 1011
AcuI CTGAAG 3 cut(s) 327, 1131, 1317
AfaI GTAC 2 cut(s) 921, 950
AfiI CCNNNNNNNGG 6 cut(s) 230, 299, 764, 1005, 1144, 1318
AflIII ACRYGT 1 cut(s) 49
AgeI ACCGGT 2 cut(s) 861, 1074
AgsI TTSAA 5 cut(s) 520, 590, 656, 955, 1349
AhdI GACNNNNNGTC 1 cut(s) 1104
AjnI CCWGG 3 cut(s) 223, 261, 895
AluBI AGCT 7 cut(s) 56, 152, 268, 889, 972, 1231, 1307
AluI AGCT 7 cut(s) 56, 152, 268, 889, 972, 1231, 1307
Alw21I GWGCWC 1 cut(s) 974
AoxI GGCC 2 cut(s) 76, 90
ApeKI GCWGC 5 cut(s) 104, 116, 152, 743, 1354
ApoI RAATTY 3 cut(s) 352, 644, 1011
AsiGI ACCGGT 2 cut(s) 861, 1074
Asp700I GAANNNNTTC 1 cut(s) 24
AspLEI GCGC 1 cut(s) 1142
AspS9I GGNCC 4 cut(s) 218, 526, 1072, 1106
AsuHPI GGTGA 4 cut(s) 22, 424, 584, 1093
AvaII GGWCC 4 cut(s) 218, 526, 1072, 1106
BanII GRGCYC 1 cut(s) 974
BbsI GAAGAC 1 cut(s) 1323
Bbv12I GWGCWC 1 cut(s) 974
BbvI GCAGC 5 cut(s) 103, 116, 139, 730, 1366
BceAI ACGGC 1 cut(s) 194
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciT130I CCWGG 3 cut(s) 225, 263, 897
BfaI CTAG 2 cut(s) 737, 1041
BisI GCNGC 5 cut(s) 105, 117, 153, 744, 1355
BlsI GCNGC 5 cut(s) 106, 118, 154, 745, 1356
Bme1390I CCNGG 3 cut(s) 225, 263, 897
Bme18I GGWCC 4 cut(s) 218, 526, 1072, 1106
BmeRI GACNNNNNGTC 1 cut(s) 1104
BmgT120I GGNCC 4 cut(s) 218, 526, 1072, 1106
BmiI GGNNCC 3 cut(s) 282, 508, 528
BmrFI CCNGG 3 cut(s) 225, 263, 897
BmsI GCATC 3 cut(s) 619, 730, 1312
BpiI GAAGAC 1 cut(s) 1323
BplI GAGNNNNNCTC 2 cut(s) 929, 961
BpmI CTGGAG 1 cut(s) 918
BpuEI CTTGAG 2 cut(s) 107, 744
BsaAI YACGTR 1 cut(s) 377
BsaJI CCNNGG 4 cut(s) 79, 110, 261, 1186
BsaWI WCCGGW 2 cut(s) 861, 1074
Bsc4I CCNNNNNNNGG 6 cut(s) 230, 299, 764, 1005, 1144, 1318
Bse118I RCCGGY 2 cut(s) 861, 1074
Bse1I ACTGG 3 cut(s) 235, 249, 1161
BseBI CCWGG 3 cut(s) 225, 263, 897
BseDI CCNNGG 4 cut(s) 79, 110, 261, 1186
BseGI GGATG 2 cut(s) 724, 1327
BseLI CCNNNNNNNGG 6 cut(s) 230, 299, 764, 1005, 1144, 1318
BseNI ACTGG 3 cut(s) 235, 249, 1161
BseRI GAGGAG 1 cut(s) 1184
BseXI GCAGC 5 cut(s) 103, 116, 139, 730, 1366
BsgI GTGCAG 2 cut(s) 713, 734
Bsh1236I CGCG 2 cut(s) 51, 1140
BshFI GGCC 2 cut(s) 78, 92
BshTI ACCGGT 2 cut(s) 861, 1074
BsiHKAI GWGCWC 1 cut(s) 974
BsiSI CCGG 2 cut(s) 862, 1075
BslFI GGGAC 1 cut(s) 1042
BslI CCNNNNNNNGG 6 cut(s) 230, 299, 764, 1005, 1144, 1318
BsmFI GGGAC 1 cut(s) 1042
BsmI GAATGC 1 cut(s) 754
BsnI GGCC 2 cut(s) 78, 92
Bsp1286I GDGCHC 1 cut(s) 974
Bsp143I GATC 2 cut(s) 649, 733
BspACI CCGC 2 cut(s) 884, 1138
BspANI GGCC 2 cut(s) 78, 92
BspFNI CGCG 2 cut(s) 51, 1140
BspLI GGNNCC 3 cut(s) 282, 508, 528
BspQI GCTCTTC 2 cut(s) 94, 210
BsrFI RCCGGY 2 cut(s) 861, 1074
BsrI ACTGG 3 cut(s) 235, 249, 1161
BssAI RCCGGY 2 cut(s) 861, 1074
BssECI CCNNGG 4 cut(s) 79, 110, 261, 1186
BssMI GATC 2 cut(s) 649, 733
BssT1I CCWWGG 3 cut(s) 79, 110, 1186
Bst2UI CCWGG 3 cut(s) 225, 263, 897
Bst4CI ACNGT 2 cut(s) 499, 599
Bst6I CTCTTC 5 cut(s) 15, 94, 210, 657, 1107
BstBAI YACGTR 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 121, 1221, 1246
BstF5I GGATG 2 cut(s) 724, 1327
BstFNI CGCG 2 cut(s) 51, 1140
BstHHI GCGC 1 cut(s) 1142
BstKTI GATC 2 cut(s) 652, 736
BstMBI GATC 2 cut(s) 649, 733
BstMWI GCNNNNNNNGC 5 cut(s) 113, 149, 200, 749, 1139
BstNI CCWGG 3 cut(s) 225, 263, 897
BstNSI RCATGY 1 cut(s) 1223
BstSCI CCNGG 3 cut(s) 223, 261, 895
BstSNI TACGTA 1 cut(s) 377
BstUI CGCG 2 cut(s) 51, 1140
BstV1I GCAGC 5 cut(s) 103, 116, 139, 730, 1366
BstV2I GAAGAC 1 cut(s) 1323
BstXI CCANNNNNNTGG 1 cut(s) 269
BsuRI GGCC 2 cut(s) 78, 92
BtsCI GGATG 2 cut(s) 724, 1327
BtsIMutI CAGTG 2 cut(s) 242, 604
Cac8I GCNNGC 3 cut(s) 121, 1221, 1246
CfoI GCGC 1 cut(s) 1142
Cfr10I RCCGGY 2 cut(s) 861, 1074
Cfr13I GGNCC 4 cut(s) 218, 526, 1072, 1106
CseI GACGC 1 cut(s) 551
Csp6I GTAC 2 cut(s) 920, 949
CspAI ACCGGT 2 cut(s) 861, 1074
CviAII CATG 3 cut(s) 476, 1049, 1220
CviQI GTAC 2 cut(s) 920, 949
DpnI GATC 2 cut(s) 651, 735
DpnII GATC 2 cut(s) 649, 733
DriI GACNNNNNGTC 1 cut(s) 1104
Eam1104I CTCTTC 5 cut(s) 15, 94, 210, 657, 1107
Eam1105I GACNNNNNGTC 1 cut(s) 1104
EarI CTCTTC 5 cut(s) 15, 94, 210, 657, 1107
Ecl136II GAGCTC 1 cut(s) 972
Eco105I TACGTA 1 cut(s) 377
Eco130I CCWWGG 3 cut(s) 79, 110, 1186
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 974
Eco47I GGWCC 4 cut(s) 218, 526, 1072, 1106
Eco53kI GAGCTC 1 cut(s) 972
Eco57I CTGAAG 3 cut(s) 327, 1131, 1317
EcoICRI GAGCTC 1 cut(s) 972
EcoRI GAATTC 1 cut(s) 644
EcoRII CCWGG 3 cut(s) 223, 261, 895
EcoT14I CCWWGG 3 cut(s) 79, 110, 1186
EcoT22I ATGCAT 1 cut(s) 634
EcoT38I GRGCYC 1 cut(s) 974
ErhI CCWWGG 3 cut(s) 79, 110, 1186
FaeI CATG 3 cut(s) 479, 1052, 1223
FaqI GGGAC 1 cut(s) 1042
FatI CATG 3 cut(s) 475, 1048, 1219
FauI CCCGC 1 cut(s) 1145
FauNDI CATATG 2 cut(s) 924, 1102
FblI GTMKAC 1 cut(s) 373
Fnu4HI GCNGC 5 cut(s) 105, 117, 153, 744, 1355
FokI GGATG 2 cut(s) 731, 1334
FriOI GRGCYC 1 cut(s) 974
Fsp4HI GCNGC 5 cut(s) 105, 117, 153, 744, 1355
FspBI CTAG 2 cut(s) 737, 1041
GlaI GCGC 1 cut(s) 1141
GluI GCNGC 5 cut(s) 105, 117, 153, 744, 1355
GsuI CTGGAG 1 cut(s) 918
HaeIII GGCC 2 cut(s) 78, 92
HapII CCGG 2 cut(s) 862, 1075
HgaI GACGC 1 cut(s) 551
HhaI GCGC 1 cut(s) 1142
Hin1II CATG 3 cut(s) 479, 1052, 1223
Hin6I GCGC 1 cut(s) 1140
HinP1I GCGC 1 cut(s) 1140
HincII GTYRAC 2 cut(s) 859, 1063
HindII GTYRAC 2 cut(s) 859, 1063
HinfI GANTC 1 cut(s) 955
HpaI GTTAAC 1 cut(s) 859
HpaII CCGG 2 cut(s) 862, 1075
HphI GGTGA 4 cut(s) 22, 424, 584, 1093
Hpy166II GTNNAC 5 cut(s) 33, 374, 694, 859, 1063
Hpy188I TCNGA 5 cut(s) 307, 322, 681, 795, 1091
Hpy188III TCNNGA 6 cut(s) 86, 653, 737, 1109, 1319, 1349
Hpy8I GTNNAC 5 cut(s) 33, 374, 694, 859, 1063
HpyAV CCTTC 3 cut(s) 398, 697, 869
HpyCH4III ACNGT 2 cut(s) 499, 599
HpyCH4IV ACGT 3 cut(s) 376, 1020, 1208
HpyCH4V TGCA 8 cut(s) 632, 715, 730, 752, 1017, 1244, 1284, 1325
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 149, 200, 749, 1139
HpySE526I ACGT 3 cut(s) 376, 1020, 1208
Hsp92II CATG 3 cut(s) 479, 1052, 1223
HspAI GCGC 1 cut(s) 1140
KspAI GTTAAC 1 cut(s) 859
Kzo9I GATC 2 cut(s) 649, 733
LguI GCTCTTC 2 cut(s) 94, 210
LmnI GCTCC 2 cut(s) 265, 886
Lsp1109I GCAGC 5 cut(s) 103, 116, 139, 730, 1366
LweI GCATC 3 cut(s) 619, 730, 1312
MaeI CTAG 2 cut(s) 737, 1041
MaeII ACGT 3 cut(s) 376, 1020, 1208
MaeIII GTNAC 3 cut(s) 412, 445, 721
MalI GATC 2 cut(s) 651, 735
MboI GATC 2 cut(s) 649, 733
MfeI CAATTG 1 cut(s) 515
MhlI GDGCHC 1 cut(s) 974
MluCI AATT 5 cut(s) 352, 515, 644, 819, 1011
MluI ACGCGT 1 cut(s) 49
MmeI TCCRAC 2 cut(s) 504, 1050
Mph1103I ATGCAT 1 cut(s) 634
MroXI GAANNNNTTC 1 cut(s) 24
MseI TTAA 7 cut(s) 72, 327, 344, 491, 552, 858, 1214
MspI CCGG 2 cut(s) 862, 1075
MspR9I CCNGG 3 cut(s) 225, 263, 897
MunI CAATTG 1 cut(s) 515
Mva1269I GAATGC 1 cut(s) 754
MvaI CCWGG 3 cut(s) 225, 263, 897
MvnI CGCG 2 cut(s) 51, 1140
MwoI GCNNNNNNNGC 5 cut(s) 113, 149, 200, 749, 1139
NdeI CATATG 2 cut(s) 924, 1102
NdeII GATC 2 cut(s) 649, 733
NlaIII CATG 3 cut(s) 479, 1052, 1223
NlaIV GGNNCC 3 cut(s) 282, 508, 528
NmuCI GTSAC 2 cut(s) 412, 445
NsiI ATGCAT 1 cut(s) 634
NspI RCATGY 1 cut(s) 1223
PaeI GCATGC 1 cut(s) 1223
PceI AGGCCT 1 cut(s) 78
PciSI GCTCTTC 2 cut(s) 94, 210
PctI GAATGC 1 cut(s) 754
PdmI GAANNNNTTC 1 cut(s) 24
PfeI GAWTC 1 cut(s) 955
PflMI CCANNNNNTGG 1 cut(s) 299
PfoI TCCNGGA 2 cut(s) 223, 895
PinAI ACCGGT 2 cut(s) 861, 1074
PkrI GCNGC 5 cut(s) 106, 118, 154, 745, 1356
Ppu21I YACGTR 1 cut(s) 377
Psp124BI GAGCTC 1 cut(s) 974
Psp6I CCWGG 3 cut(s) 223, 261, 895
PspGI CCWGG 3 cut(s) 223, 261, 895
PspN4I GGNNCC 3 cut(s) 282, 508, 528
PspPI GGNCC 4 cut(s) 218, 526, 1072, 1106
RsaI GTAC 2 cut(s) 921, 950
RsaNI GTAC 2 cut(s) 920, 949
SacI GAGCTC 1 cut(s) 974
SapI GCTCTTC 2 cut(s) 94, 210
SaqAI TTAA 7 cut(s) 72, 327, 344, 491, 552, 858, 1214
SatI GCNGC 5 cut(s) 105, 117, 153, 744, 1355
Sau3AI GATC 2 cut(s) 649, 733
Sau96I GGNCC 4 cut(s) 218, 526, 1072, 1106
ScrFI CCNGG 3 cut(s) 225, 263, 897
SduI GDGCHC 1 cut(s) 974
SfaNI GCATC 3 cut(s) 619, 730, 1312
SinI GGWCC 4 cut(s) 218, 526, 1072, 1106
SmlI CTYRAG 2 cut(s) 86, 759
SmoI CTYRAG 2 cut(s) 86, 759
SnaBI TACGTA 1 cut(s) 377
SphI GCATGC 1 cut(s) 1223
Sse9I AATT 5 cut(s) 352, 515, 644, 819, 1011
SseBI AGGCCT 1 cut(s) 78
SsiI CCGC 2 cut(s) 884, 1138
SspI AATATT 1 cut(s) 993
SspMI CTAG 2 cut(s) 737, 1041
SstI GAGCTC 1 cut(s) 974
StuI AGGCCT 1 cut(s) 78
StyD4I CCNGG 3 cut(s) 223, 261, 895
StyI CCWWGG 3 cut(s) 79, 110, 1186
TaaI ACNGT 2 cut(s) 499, 599
TaiI ACGT 3 cut(s) 379, 1023, 1211
TaqI TCGA 2 cut(s) 168, 648
TasI AATT 5 cut(s) 352, 515, 644, 819, 1011
TfiI GAWTC 1 cut(s) 955
Tru1I TTAA 7 cut(s) 72, 327, 344, 491, 552, 858, 1214
Tru9I TTAA 7 cut(s) 72, 327, 344, 491, 552, 858, 1214
TscAI CASTG 2 cut(s) 249, 604
TseFI GTSAC 2 cut(s) 412, 445
TseI GCWGC 5 cut(s) 104, 116, 152, 743, 1354
Tsp45I GTSAC 2 cut(s) 412, 445
TspDTI ATGAA 6 cut(s) 306, 417, 815, 888, 898, 1277
TspGWI ACGGA 3 cut(s) 452, 656, 839
TspRI CASTG 2 cut(s) 249, 604
Van91I CCANNNNNTGG 1 cut(s) 299
VpaK11BI GGWCC 4 cut(s) 218, 526, 1072, 1106
XapI RAATTY 3 cut(s) 352, 644, 1011
XbaI TCTAGA 1 cut(s) 736
XceI RCATGY 1 cut(s) 1223
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 373
XmnI GAANNNNTTC 1 cut(s) 24
XspI CTAG 2 cut(s) 737, 1041
Zsp2I ATGCAT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.