Rh5AG406600

Holliday junction ATP-dependent DNA helicase ruvB. Source PGD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002367
Physical Location & Seq
Reverse (-)
70312634 .. 70314499
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG406600.1

Sequence Viewer

Length: 396 bp
ATGCTGCTGCTCTCTCTTTTCTCTAGAGTATTGAAACTCACGTTTAAAGGTGTATCAATGGGTTCAGCCTTACAGAATAAAAAGCAAAACTTATATCATACCAGTATTGGGAGTTCTCTGCAAAACCCAGAAGAGTTAAGAGAGATGGACAAGGTGAAAATAGAAGAGGTTCAGTCACCTACCAAGAAGCAGCGCATAGCTACTCATACACACACCAAAGGCCTTGGTCTTAAGGTTCCATTCTGCCCAATGGTTGGGTCTGAAGTATATTCATCAGAAGGTAAGAAAACAGAGGTACTGATGGAAAGTTTTAGACGGGCTATTAATCTACTTATCAAGGGAATTAAAGAGGTCTATGAAGGAGAGGTGACAGAACTAACACTAGAAGAAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIP49

Protein Analysis

131

Amino Acids

14.76

Weight (kDa)

8.99

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIP49 PF06068 63 - 130 7.2e-19 TIP49 P-loop domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 282
AfaI GTAC 1 cut(s) 297
AfiI CCNNNNNNNGG 2 cut(s) 108, 254
AflII CTTAAG 1 cut(s) 230
AgsI TTSAA 1 cut(s) 34
AluBI AGCT 1 cut(s) 200
AluI AGCT 1 cut(s) 200
AoxI GGCC 1 cut(s) 220
ApeKI GCWGC 3 cut(s) 4, 7, 190
AseI ATTAAT 1 cut(s) 324
Asp700I GAANNNNTTC 1 cut(s) 168
AspLEI GCGC 1 cut(s) 195
AsuHPI GGTGA 3 cut(s) 166, 168, 379
BbvI GCAGC 1 cut(s) 202
BccI CCATC 2 cut(s) 139, 295
BfaI CTAG 2 cut(s) 24, 383
BfrI CTTAAG 1 cut(s) 230
BisI GCNGC 3 cut(s) 5, 8, 191
BlsI GCNGC 3 cut(s) 6, 9, 192
BmiI GGNNCC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 223
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 254
Bse1I ACTGG 1 cut(s) 102
BseDI CCNNGG 1 cut(s) 223
BseLI CCNNNNNNNGG 2 cut(s) 108, 254
BseNI ACTGG 1 cut(s) 102
BseXI GCAGC 1 cut(s) 202
BshFI GGCC 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 108, 254
BsnI GGCC 1 cut(s) 222
BspANI GGCC 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 237
BspTI CTTAAG 1 cut(s) 230
BsrI ACTGG 1 cut(s) 102
BssECI CCNNGG 1 cut(s) 223
BssT1I CCWWGG 1 cut(s) 223
Bst6I CTCTTC 2 cut(s) 126, 159
BstAFI CTTAAG 1 cut(s) 230
BstHHI GCGC 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 202
BsuRI GGCC 1 cut(s) 222
CfoI GCGC 1 cut(s) 195
Csp6I GTAC 1 cut(s) 296
CviJI RGCY 4 cut(s) 68, 200, 222, 320
CviKI_1 RGCY 4 cut(s) 68, 200, 222, 320
CviQI GTAC 1 cut(s) 296
DraI TTTAAA 1 cut(s) 46
Eam1104I CTCTTC 2 cut(s) 126, 159
EarI CTCTTC 2 cut(s) 126, 159
Eco130I CCWWGG 1 cut(s) 223
Eco147I AGGCCT 1 cut(s) 222
Eco57I CTGAAG 1 cut(s) 282
EcoT14I CCWWGG 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 223
FaiI YATR 6 cut(s) 94, 99, 197, 207, 268, 357
FalI AAGNNNNNCTT 2 cut(s) 74, 106
Fnu4HI GCNGC 3 cut(s) 5, 8, 191
Fsp4HI GCNGC 3 cut(s) 5, 8, 191
FspBI CTAG 2 cut(s) 24, 383
GlaI GCGC 1 cut(s) 194
GluI GCNGC 3 cut(s) 5, 8, 191
HaeIII GGCC 1 cut(s) 222
HhaI GCGC 1 cut(s) 195
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HphI GGTGA 3 cut(s) 166, 168, 379
Hpy188I TCNGA 2 cut(s) 262, 277
Hpy188III TCNNGA 1 cut(s) 24
HpyAV CCTTC 2 cut(s) 272, 353
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 1 cut(s) 121
HpySE526I ACGT 1 cut(s) 41
HspAI GCGC 1 cut(s) 193
LpnPI CCDG 2 cut(s) 115, 141
Lsp1109I GCAGC 1 cut(s) 202
MaeI CTAG 2 cut(s) 24, 383
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 2 cut(s) 174, 367
MboII GAAGA 2 cut(s) 143, 176
MluCI AATT 1 cut(s) 342
MnlI CCTC 4 cut(s) 160, 286, 343, 358
MroXI GAANNNNTTC 1 cut(s) 168
MseI TTAA 5 cut(s) 45, 137, 231, 324, 345
MspCI CTTAAG 1 cut(s) 230
NlaIV GGNNCC 1 cut(s) 237
NmuCI GTSAC 2 cut(s) 174, 367
PceI AGGCCT 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 168
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 3 cut(s) 6, 9, 192
PshBI ATTAAT 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 237
PsrI GAACNNNNNNTAC 2 cut(s) 97, 129
RsaI GTAC 1 cut(s) 297
RsaNI GTAC 1 cut(s) 296
SaqAI TTAA 5 cut(s) 45, 137, 231, 324, 345
SatI GCNGC 3 cut(s) 5, 8, 191
SgeI CNNG 9 cut(s) 36, 52, 114, 140, 163, 196, 236, 329, 349
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 1 cut(s) 342
SseBI AGGCCT 1 cut(s) 222
SspMI CTAG 2 cut(s) 24, 383
StuI AGGCCT 1 cut(s) 222
StyI CCWWGG 1 cut(s) 223
TaiI ACGT 1 cut(s) 44
TasI AATT 1 cut(s) 342
Tru1I TTAA 5 cut(s) 45, 137, 231, 324, 345
Tru9I TTAA 5 cut(s) 45, 137, 231, 324, 345
TseFI GTSAC 2 cut(s) 174, 367
TseI GCWGC 3 cut(s) 4, 7, 190
Tsp45I GTSAC 2 cut(s) 174, 367
TspDTI ATGAA 2 cut(s) 261, 372
Van91I CCANNNNNTGG 1 cut(s) 254
Vha464I CTTAAG 1 cut(s) 230
VspI ATTAAT 1 cut(s) 324
XbaI TCTAGA 1 cut(s) 23
XmnI GAANNNNTTC 1 cut(s) 168
XspI CTAG 2 cut(s) 24, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.