Rroxscaffold_2G00125770

Belongs to the RuvB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002367
Physical Location & Seq
Reverse (-)
60830097 .. 60833595
3499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00125770.1

Sequence Viewer

Length: 525 bp
ATGGTTGGGTCTGAAGCATATTCATCAGAAGTTGAGATAACAGAGGTTCTAATGGAAAATTTTAGACGGGCTATTGATCTACTTATCAAGGAAAATAAAGAGGTGACAGAACCAACACCAGAAGGAACAGATAGTATTACAGGTGGCTATGGTAAAAGCATTAGCCATGTAATAATTGGATTAAAAACTGCCAAAGGAACCAAGCAACGGAGACCTGAGTTTTCTCCGGTGAGTTCCGAGAACGTGCGGTGGAACTGGTCCTCTTTTAAGTGGGCCGCGAGAGAGCAGAGCTTGAGTCAGAGTAGGGTGCTTCGCGTTCAGTTCCGTCATACTTTGAAAACCAAGATGGGCTCCGCTGGATTGGTTCCAACGGGTCTAACCGGTTTAGCACAACTCGGCTCGTTAATTTGCGGGTTTTTGGCGTGCGAGTTTTTCTCACCATTAATAAATGGGCGGGTTCATGCAGCTTTTAGCGTGCTGGTTTCGTGTGAGTTTGCGGGTTACGGGTTCAAATGCCAGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000491 GO:0000492 GO:0000785 GO:0000790 GO:0000812 GO:0002682 GO:0002831 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009888 GO:0009889 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010604 GO:0010628 GO:0010755 GO:0010756 GO:0010941 GO:0010954 GO:0016043 GO:0016363 GO:0016462 GO:0016569 GO:0016570 GO:0016573 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0030162 GO:0031011 GO:0031248 GO:0031323 GO:0031325 GO:0031326 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032270 GO:0032392 GO:0032502 GO:0032508 GO:0032991 GO:0033202 GO:0034399 GO:0034622 GO:0034708 GO:0035097 GO:0035267 GO:0036211 GO:0042623 GO:0042981 GO:0043067 GO:0043138 GO:0043140 GO:0043170 GO:0043189 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043900 GO:0043933 GO:0043967 GO:0043968 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044665 GO:0045088 GO:0045862 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048856 GO:0050776 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070035 GO:0070603 GO:0070613 GO:0071103 GO:0071339 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0080134 GO:0097255 GO:0097346 GO:0140097 GO:1900150 GO:1901564 GO:1902493 GO:1902494 GO:1902562 GO:1903317 GO:1903319 GO:1903506 GO:1904949 GO:1990234 GO:2000072 GO:2000112 GO:2000269 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
TIP49

Protein Analysis

174

Amino Acids

19.1

Weight (kDa)

8.96

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIP49 PF06068 1 - 69 7.5e-11 TIP49 P-loop domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 278, 315
AciI CCGC 6 cut(s) 247, 276, 354, 411, 454, 497
AcsI RAATTY 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 207
AgeI ACCGGT 1 cut(s) 380
AgsI TTSAA 2 cut(s) 337, 511
AjnI CCWGG 1 cut(s) 516
AluBI AGCT 2 cut(s) 291, 467
AluI AGCT 2 cut(s) 291, 467
Alw26I GTCTC 1 cut(s) 205
AoxI GGCC 2 cut(s) 273, 519
ApeKI GCWGC 1 cut(s) 464
ApoI RAATTY 1 cut(s) 58
AseI ATTAAT 1 cut(s) 443
AsiGI ACCGGT 1 cut(s) 380
AspS9I GGNCC 2 cut(s) 258, 273
AsuHPI GGTGA 3 cut(s) 115, 241, 429
AvaII GGWCC 1 cut(s) 258
BanII GRGCYC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 476
BccI CCATC 1 cut(s) 340
BciT130I CCWGG 1 cut(s) 518
BcoDI GTCTC 1 cut(s) 205
BisI GCNGC 2 cut(s) 276, 465
BlsI GCNGC 2 cut(s) 277, 466
Bme1390I CCNGG 1 cut(s) 518
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 2 cut(s) 258, 273
BmiI GGNNCC 3 cut(s) 199, 352, 366
BmrFI CCNGG 1 cut(s) 518
BplI GAGNNNNNCTC 2 cut(s) 419, 451
BpuEI CTTGAG 1 cut(s) 313
BsaI GGTCTC 1 cut(s) 205
BsaWI WCCGGW 2 cut(s) 226, 380
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse118I RCCGGY 1 cut(s) 380
Bse1I ACTGG 1 cut(s) 260
BseBI CCWGG 1 cut(s) 518
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 207
BseNI ACTGG 1 cut(s) 260
BseXI GCAGC 1 cut(s) 476
Bsh1236I CGCG 2 cut(s) 278, 315
BshFI GGCC 2 cut(s) 275, 521
BshTI ACCGGT 1 cut(s) 380
BsiSI CCGG 2 cut(s) 227, 381
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 1 cut(s) 205
BsnI GGCC 2 cut(s) 275, 521
Bso31I GGTCTC 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 1 cut(s) 76
BspACI CCGC 6 cut(s) 247, 276, 354, 411, 454, 497
BspANI GGCC 2 cut(s) 275, 521
BspCNI CTCAG 1 cut(s) 208
BspFNI CGCG 2 cut(s) 278, 315
BspLI GGNNCC 3 cut(s) 199, 352, 366
BspTNI GGTCTC 1 cut(s) 205
BsrFI RCCGGY 1 cut(s) 380
BsrI ACTGG 1 cut(s) 260
BssAI RCCGGY 1 cut(s) 380
BssMI GATC 1 cut(s) 76
Bst2UI CCWGG 1 cut(s) 518
BstC8I GCNNGC 2 cut(s) 424, 476
BstDEI CTNAG 1 cut(s) 216
BstFNI CGCG 2 cut(s) 278, 315
BstKTI GATC 1 cut(s) 79
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 1 cut(s) 76
BstNI CCWGG 1 cut(s) 518
BstSCI CCNGG 1 cut(s) 516
BstUI CGCG 2 cut(s) 278, 315
BstV1I GCAGC 1 cut(s) 476
BsuRI GGCC 2 cut(s) 275, 521
Cac8I GCNNGC 2 cut(s) 424, 476
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 2 cut(s) 258, 273
CspAI ACCGGT 1 cut(s) 380
CviAII CATG 2 cut(s) 167, 461
CviJI RGCY 9 cut(s) 71, 147, 165, 275, 291, 351, 399, 467, 521
CviKI_1 RGCY 9 cut(s) 71, 147, 165, 275, 291, 351, 399, 467, 521
DdeI CTNAG 1 cut(s) 216
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
Eco147I AGGCCT 1 cut(s) 521
Eco24I GRGCYC 1 cut(s) 353
Eco31I GGTCTC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 258
Eco57I CTGAAG 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 516
EcoT38I GRGCYC 1 cut(s) 353
FaeI CATG 2 cut(s) 170, 464
FaiI YATR 5 cut(s) 19, 150, 168, 330, 462
FatI CATG 2 cut(s) 166, 460
FauI CCCGC 3 cut(s) 404, 447, 490
Fnu4HI GCNGC 2 cut(s) 276, 465
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 2 cut(s) 276, 465
GluI GCNGC 2 cut(s) 276, 465
HaeIII GGCC 2 cut(s) 275, 521
HapII CCGG 2 cut(s) 227, 381
Hin1II CATG 2 cut(s) 170, 464
HinfI GANTC 1 cut(s) 295
HpaII CCGG 2 cut(s) 227, 381
HphI GGTGA 3 cut(s) 115, 241, 429
Hpy188I TCNGA 4 cut(s) 13, 28, 238, 300
HpyAV CCTTC 1 cut(s) 116
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 1 cut(s) 464
HpyF3I CTNAG 1 cut(s) 216
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 2 cut(s) 170, 464
Kzo9I GATC 1 cut(s) 76
LmnI GCTCC 1 cut(s) 356
LpnPI CCDG 9 cut(s) 126, 132, 228, 240, 241, 342, 394, 464, 503
Lsp1109I GCAGC 1 cut(s) 476
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 2 cut(s) 103, 500
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 3 cut(s) 58, 174, 405
MlyI GAGTC 1 cut(s) 304
MmeI TCCRAC 1 cut(s) 392
MnlI CCTC 3 cut(s) 37, 94, 271
MseI TTAA 4 cut(s) 182, 267, 404, 443
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 2 cut(s) 227, 381
MspR9I CCNGG 1 cut(s) 518
MvaI CCWGG 1 cut(s) 518
MvnI CGCG 2 cut(s) 278, 315
NdeII GATC 1 cut(s) 76
NlaIII CATG 2 cut(s) 170, 464
NlaIV GGNNCC 3 cut(s) 199, 352, 366
NmeAIII GCCGAG 1 cut(s) 375
NmuCI GTSAC 1 cut(s) 103
PceI AGGCCT 1 cut(s) 521
PinAI ACCGGT 1 cut(s) 380
PkrI GCNGC 2 cut(s) 277, 466
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
PshBI ATTAAT 1 cut(s) 443
Psp6I CCWGG 1 cut(s) 516
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 3 cut(s) 199, 352, 366
PspPI GGNCC 2 cut(s) 258, 273
SaqAI TTAA 4 cut(s) 182, 267, 404, 443
SatI GCNGC 2 cut(s) 276, 465
Sau3AI GATC 1 cut(s) 76
Sau96I GGNCC 2 cut(s) 258, 273
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 1 cut(s) 518
SduI GDGCHC 1 cut(s) 353
SetI ASST 7 cut(s) 48, 105, 145, 217, 246, 293, 469
SinI GGWCC 1 cut(s) 258
SmlI CTYRAG 1 cut(s) 292
SmoI CTYRAG 1 cut(s) 292
Sse9I AATT 3 cut(s) 58, 174, 405
SseBI AGGCCT 1 cut(s) 521
SsiI CCGC 6 cut(s) 247, 276, 354, 411, 454, 497
StuI AGGCCT 1 cut(s) 521
StyD4I CCNGG 1 cut(s) 516
TaiI ACGT 1 cut(s) 246
TasI AATT 3 cut(s) 58, 174, 405
TauI GCSGC 1 cut(s) 278
Tru1I TTAA 4 cut(s) 182, 267, 404, 443
Tru9I TTAA 4 cut(s) 182, 267, 404, 443
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 1 cut(s) 464
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 2 cut(s) 12, 449
TspGWI ACGGA 2 cut(s) 223, 314
VpaK11BI GGWCC 1 cut(s) 258
VspI ATTAAT 1 cut(s) 443
XapI RAATTY 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.