MD03G1150000.v1.1

Belongs to the RuvB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002367
Physical Location & Seq
Forward (+)
16698395 .. 16703594
5200 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1150000.v1.1.491

Sequence Viewer

Length: 1377 bp
ATGGACAAGGTGAGGATAGAAGAGGTTCAGTCGACTACCAAGAAGCAGCGCATAGCCACTCACACCCACATCAAAGGCCTTGGTCTTGAGGCCAATGGAAGAGCAATACCTTGGGCTGCTGGCTTTGTGGGTCAGGGGGAAGCAAGAGAAGCTGCTGGCCTTGTTGTTGATATGATACGGCAGAAGAAGATGGCTGGTCGGGCACTTCTACTTGCTGGACCTCCTGGAACAGGAAAGACAGCGCTAGCTCTTGGAATATCTCAAGAGCTTGGGAGTAAGGTTCCGTTCTGCCCGATGGTTGGATCAGAAGTATACTCATCAGAAGTTAAGAAAACTGAGGTTTTAATGGAAAATTTTAGACGGGCTATTGGTCTACGTATCAAGGAAAATAAAGAGGTGTATGAAGGAGAGGTGACGGAACTCACACCAGAAGAAACAGAGAGTGTAACCGGTGGTTATGGTAAAAGCATTAGTCACGTCATCATTGGATTAAAAACTGTTAAAGGAACTAAGCAACTGAAGTTGGACCCCACTATTTATGATGCATTGATTAAGGAAAAGGTAGCTGTTGGTGATGTTATATACATTGAAGCAAATAGCGGAGCAGTTAAAAGAGTTGGTAGAAGTGATGCTTTTGCTACAGAGTTTGATCTGGAAGCAGAAGAGTATGTACCACTTCCAAAAGGAGAGGTTCACAAAAAGAAGGAGATTGTGCAGGATGTAACACTACATGATCTGGATGCTGCAAATGCGCGGCCTCAAGGTGGGCAAGACATTTTATCTCTAATGGGTCAAATGATGAAGCCGAGGAAAACAGAAATTACTGACAAGTTGCGACAAGAAATAAATAAGGTTGTTAACCGGTATATTGATGAAGGTGTAGCGGAACTTGTGCCTGGAGTTCTGTTCATTGATGAGGTACATATGCTGGATATGGAGTGCTTTTCATACTTGAACCGTGCTTTGGAGAGCTCGTTATCTCCAATCGTAATATTTGCCACCAATAGAGGAATTTGCAATGTAAGAGGGACTGATATGGCTAGTCCTCATGGAATTCCCGTTGACTTGTTGGACCGGTTGGTGATTATCCGAACACAGACTTACGGTCTTGAAGAGATGATAAAGATCTTAGTAATTCGTGCACAAGTAGAGGAACTGGCTATAGATGATGACAGTTTGGCTTACCTCGGAGAGATAGGACAAAAATCATCTTTAAGGCATGCTGTTCAGCTCTTATCGCCTGCAAGCATTGTGGCAAAAATGAATGGCAGAGACAAAATATGCAAGGCTGACCTAGAGGAGGTGGGTACGCTTTATCTGGATGCCAAGTCTTCGGCAAAGGTTCTTCAAGAGCAGCAGGAAAAGTACATTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000491 GO:0000492 GO:0000785 GO:0000790 GO:0000812 GO:0002682 GO:0002831 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009888 GO:0009889 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010604 GO:0010628 GO:0010755 GO:0010756 GO:0010941 GO:0010954 GO:0016043 GO:0016363 GO:0016462 GO:0016569 GO:0016570 GO:0016573 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0030162 GO:0031011 GO:0031248 GO:0031323 GO:0031325 GO:0031326 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032270 GO:0032392 GO:0032502 GO:0032508 GO:0032991 GO:0033202 GO:0034399 GO:0034622 GO:0034708 GO:0035097 GO:0035267 GO:0036211 GO:0042623 GO:0042981 GO:0043067 GO:0043138 GO:0043140 GO:0043170 GO:0043189 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043900 GO:0043933 GO:0043967 GO:0043968 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044665 GO:0045088 GO:0045862 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048856 GO:0050776 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070035 GO:0070603 GO:0070613 GO:0071103 GO:0071339 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0080134 GO:0097255 GO:0097346 GO:0140097 GO:1900150 GO:1901564 GO:1902493 GO:1902494 GO:1902562 GO:1903317 GO:1903319 GO:1903506 GO:1904949 GO:1990234 GO:2000072 GO:2000112 GO:2000269 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
TIP49

Protein Analysis

459

Amino Acids

50.28

Weight (kDa)

5.8

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIP49 PF06068 17 - 368 8.6e-167 TIP49 P-loop domain
RuvB_N PF05496 41 - 94 1.2e-06 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 69 - 118 1.2e-06 ATPase family associated with various cellular activities (AAA)
TIP49_C PF17856 376 - 441 2.4e-18 TIP49 AAA-lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 32, 312, 373
AccII CGCG 1 cut(s) 754
AciI CCGC 3 cut(s) 600, 754, 884
AclWI GGATC 1 cut(s) 310
AcsI RAATTY 3 cut(s) 352, 1011, 1053
AcuI CTGAAG 1 cut(s) 539
AfaI GTAC 4 cut(s) 672, 921, 1309, 1367
AfeI AGCGCT 1 cut(s) 243
AfiI CCNNNNNNNGG 5 cut(s) 230, 299, 764, 964, 1300
AgeI ACCGGT 3 cut(s) 449, 861, 1074
AgsI TTSAA 4 cut(s) 590, 955, 1112, 1349
AhdI GACNNNNNGTC 1 cut(s) 1104
AjiI CACGTC 1 cut(s) 478
AjnI CCWGG 2 cut(s) 223, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 947, 979
AluBI AGCT 6 cut(s) 152, 248, 268, 566, 972, 1231
AluI AGCT 6 cut(s) 152, 248, 268, 566, 972, 1231
Alw21I GWGCWC 2 cut(s) 974, 1144
Alw26I GTCTC 1 cut(s) 1266
Alw44I GTGCAC 1 cut(s) 1140
AlwI GGATC 1 cut(s) 310
Aor51HI AGCGCT 1 cut(s) 243
AoxI GGCC 4 cut(s) 76, 90, 157, 755
ApaLI GTGCAC 1 cut(s) 1140
ApeKI GCWGC 5 cut(s) 46, 116, 152, 743, 1354
ApoI RAATTY 3 cut(s) 352, 1011, 1053
AsiGI ACCGGT 3 cut(s) 449, 861, 1074
Asp700I GAANNNNTTC 1 cut(s) 24
AspLEI GCGC 3 cut(s) 51, 244, 754
AspS9I GGNCC 3 cut(s) 218, 526, 1072
AsuHPI GGTGA 4 cut(s) 22, 424, 584, 1093
AsuNHI GCTAGC 1 cut(s) 244
AvaII GGWCC 3 cut(s) 218, 526, 1072
BaeGI GKGCMC 2 cut(s) 205, 1144
BanII GRGCYC 1 cut(s) 974
BarI GAAGNNNNNNTAC 2 cut(s) 654, 686
BbsI GAAGAC 1 cut(s) 1323
Bbv12I GWGCWC 2 cut(s) 974, 1144
BbvI GCAGC 5 cut(s) 58, 103, 139, 730, 1366
BccI CCATC 2 cut(s) 184, 289
BceAI ACGGC 1 cut(s) 194
BciT130I CCWGG 2 cut(s) 225, 897
BcoDI GTCTC 1 cut(s) 1266
BfaI CTAG 3 cut(s) 245, 1041, 1295
BfmI CTRYAG 2 cut(s) 639, 1161
BfoI RGCGCY 1 cut(s) 245
BglII AGATCT 1 cut(s) 1125
BisI GCNGC 6 cut(s) 47, 117, 153, 744, 755, 1355
BlsI GCNGC 6 cut(s) 48, 118, 154, 745, 756, 1356
Bme1390I CCNGG 2 cut(s) 225, 897
Bme18I GGWCC 3 cut(s) 218, 526, 1072
BmeRI GACNNNNNGTC 1 cut(s) 1104
BmgBI CACGTC 1 cut(s) 478
BmgT120I GGNCC 3 cut(s) 218, 526, 1072
BmiI GGNNCC 2 cut(s) 282, 528
BmrFI CCNGG 2 cut(s) 225, 897
BmsI GCATC 4 cut(s) 532, 619, 730, 1312
BmtI GCTAGC 1 cut(s) 248
BpiI GAAGAC 1 cut(s) 1323
BpmI CTGGAG 1 cut(s) 918
BpuEI CTTGAG 3 cut(s) 107, 246, 744
BsaAI YACGTR 1 cut(s) 377
BsaBI GATNNNNATC 1 cut(s) 1124
BsaJI CCNNGG 4 cut(s) 79, 110, 806, 1186
BsaWI WCCGGW 3 cut(s) 449, 861, 1074
BsaXI ACNNNNNCTCC 2 cut(s) 959, 989
Bsc4I CCNNNNNNNGG 5 cut(s) 230, 299, 764, 964, 1300
Bse118I RCCGGY 3 cut(s) 449, 861, 1074
Bse1I ACTGG 1 cut(s) 1161
Bse3DI GCAATG 1 cut(s) 1024
Bse8I GATNNNNATC 1 cut(s) 1124
BseBI CCWGG 2 cut(s) 225, 897
BseDI CCNNGG 4 cut(s) 79, 110, 806, 1186
BseGI GGATG 3 cut(s) 724, 745, 1327
BseJI GATNNNNATC 1 cut(s) 1124
BseLI CCNNNNNNNGG 5 cut(s) 230, 299, 764, 964, 1300
BseMI GCAATG 1 cut(s) 1024
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 1161
BseRI GAGGAG 1 cut(s) 1313
BseSI GKGCMC 2 cut(s) 205, 1144
BseXI GCAGC 5 cut(s) 58, 103, 139, 730, 1366
BsgI GTGCAG 1 cut(s) 734
Bsh1236I CGCG 1 cut(s) 754
BshFI GGCC 4 cut(s) 78, 92, 159, 757
BshTI ACCGGT 3 cut(s) 449, 861, 1074
BsiHKAI GWGCWC 2 cut(s) 974, 1144
BsiSI CCGG 3 cut(s) 450, 862, 1075
BslFI GGGAC 1 cut(s) 1042
BslI CCNNNNNNNGG 5 cut(s) 230, 299, 764, 964, 1300
BsmAI GTCTC 1 cut(s) 1266
BsmFI GGGAC 1 cut(s) 1042
BsnI GGCC 4 cut(s) 78, 92, 159, 757
Bsp1286I GDGCHC 3 cut(s) 205, 974, 1144
Bsp143I GATC 4 cut(s) 302, 649, 733, 1125
BspACI CCGC 3 cut(s) 600, 754, 884
BspANI GGCC 4 cut(s) 78, 92, 159, 757
BspCNI CTCAG 1 cut(s) 328
BspFNI CGCG 1 cut(s) 754
BspLI GGNNCC 2 cut(s) 282, 528
BspOI GCTAGC 1 cut(s) 248
BspPI GGATC 1 cut(s) 310
BspQI GCTCTTC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 1024
BsrFI RCCGGY 3 cut(s) 449, 861, 1074
BsrI ACTGG 1 cut(s) 1161
BssAI RCCGGY 3 cut(s) 449, 861, 1074
BssECI CCNNGG 4 cut(s) 79, 110, 806, 1186
BssMI GATC 4 cut(s) 302, 649, 733, 1125
BssNAI GTATAC 1 cut(s) 313
BssT1I CCWWGG 2 cut(s) 79, 110
Bst1107I GTATAC 1 cut(s) 313
Bst2UI CCWGG 2 cut(s) 225, 897
Bst4CI ACNGT 4 cut(s) 499, 959, 1106, 1175
Bst6I CTCTTC 4 cut(s) 15, 94, 657, 1107
BstBAI YACGTR 1 cut(s) 377
BstC8I GCNNGC 6 cut(s) 121, 157, 246, 1221, 1242, 1246
BstDEI CTNAG 3 cut(s) 336, 510, 1129
BstENI CCTNNNNNAGG 2 cut(s) 228, 1298
BstF5I GGATG 3 cut(s) 724, 745, 1327
BstFNI CGCG 1 cut(s) 754
BstH2I RGCGCY 1 cut(s) 245
BstHHI GCGC 3 cut(s) 51, 244, 754
BstKTI GATC 4 cut(s) 305, 652, 736, 1128
BstMAI GTCTC 1 cut(s) 1266
BstMBI GATC 4 cut(s) 302, 649, 733, 1125
BstMWI GCNNNNNNNGC 4 cut(s) 149, 200, 749, 1237
BstNI CCWGG 2 cut(s) 225, 897
BstNSI RCATGY 1 cut(s) 1223
BstSCI CCNGG 2 cut(s) 223, 895
BstSFI CTRYAG 2 cut(s) 639, 1161
BstSLI GKGCMC 2 cut(s) 205, 1144
BstSNI TACGTA 1 cut(s) 377
BstUI CGCG 1 cut(s) 754
BstV1I GCAGC 5 cut(s) 58, 103, 139, 730, 1366
BstV2I GAAGAC 1 cut(s) 1323
BstX2I RGATCY 1 cut(s) 1125
BstYI RGATCY 1 cut(s) 1125
BstZ17I GTATAC 1 cut(s) 313
BsuRI GGCC 4 cut(s) 78, 92, 159, 757
BtrI CACGTC 1 cut(s) 478
BtsCI GGATG 3 cut(s) 724, 745, 1327
Cac8I GCNNGC 6 cut(s) 121, 157, 246, 1221, 1242, 1246
CfoI GCGC 3 cut(s) 51, 244, 754
Cfr10I RCCGGY 3 cut(s) 449, 861, 1074
Cfr13I GGNCC 3 cut(s) 218, 526, 1072
Csp6I GTAC 4 cut(s) 671, 920, 1308, 1366
CspAI ACCGGT 3 cut(s) 449, 861, 1074
CspCI CAANNNNNGTGG 2 cut(s) 1233, 1268
CviAII CATG 3 cut(s) 731, 1049, 1220
CviQI GTAC 4 cut(s) 671, 920, 1308, 1366
DdeI CTNAG 3 cut(s) 336, 510, 1129
DpnI GATC 4 cut(s) 304, 651, 735, 1127
DpnII GATC 4 cut(s) 302, 649, 733, 1125
DriI GACNNNNNGTC 1 cut(s) 1104
Eam1104I CTCTTC 4 cut(s) 15, 94, 657, 1107
Eam1105I GACNNNNNGTC 1 cut(s) 1104
EarI CTCTTC 4 cut(s) 15, 94, 657, 1107
Ecl136II GAGCTC 1 cut(s) 972
Eco105I TACGTA 1 cut(s) 377
Eco130I CCWWGG 2 cut(s) 79, 110
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 974
Eco47I GGWCC 3 cut(s) 218, 526, 1072
Eco47III AGCGCT 1 cut(s) 243
Eco53kI GAGCTC 1 cut(s) 972
Eco57I CTGAAG 1 cut(s) 539
EcoICRI GAGCTC 1 cut(s) 972
EcoNI CCTNNNNNAGG 2 cut(s) 228, 1298
EcoRI GAATTC 1 cut(s) 1053
EcoRII CCWGG 2 cut(s) 223, 895
EcoT14I CCWWGG 2 cut(s) 79, 110
EcoT22I ATGCAT 1 cut(s) 547
EcoT38I GRGCYC 1 cut(s) 974
ErhI CCWWGG 2 cut(s) 79, 110
FaeI CATG 3 cut(s) 734, 1052, 1223
FalI AAGNNNNNCTT 2 cut(s) 616, 648
FaqI GGGAC 1 cut(s) 1042
FatI CATG 3 cut(s) 730, 1048, 1219
FauNDI CATATG 1 cut(s) 924
FblI GTMKAC 3 cut(s) 32, 312, 373
Fnu4HI GCNGC 6 cut(s) 47, 117, 153, 744, 755, 1355
FokI GGATG 3 cut(s) 731, 752, 1334
FriOI GRGCYC 1 cut(s) 974
Fsp4HI GCNGC 6 cut(s) 47, 117, 153, 744, 755, 1355
FspBI CTAG 3 cut(s) 245, 1041, 1295
GlaI GCGC 3 cut(s) 50, 243, 753
GluI GCNGC 6 cut(s) 47, 117, 153, 744, 755, 1355
GsuI CTGGAG 1 cut(s) 918
HaeII RGCGCY 1 cut(s) 245
HaeIII GGCC 4 cut(s) 78, 92, 159, 757
HapII CCGG 3 cut(s) 450, 862, 1075
HhaI GCGC 3 cut(s) 51, 244, 754
Hin1II CATG 3 cut(s) 734, 1052, 1223
Hin6I GCGC 3 cut(s) 49, 242, 752
HinP1I GCGC 3 cut(s) 49, 242, 752
HincII GTYRAC 3 cut(s) 33, 859, 1063
HindII GTYRAC 3 cut(s) 33, 859, 1063
HpaI GTTAAC 1 cut(s) 859
HpaII CCGG 3 cut(s) 450, 862, 1075
HphI GGTGA 4 cut(s) 22, 424, 584, 1093
Hpy166II GTNNAC 7 cut(s) 33, 313, 374, 694, 859, 1063, 1142
Hpy188I TCNGA 4 cut(s) 307, 322, 1091, 1190
Hpy188III TCNNGA 7 cut(s) 86, 263, 653, 737, 1109, 1319, 1349
Hpy8I GTNNAC 7 cut(s) 33, 313, 374, 694, 859, 1063, 1142
HpyAV CCTTC 3 cut(s) 398, 697, 869
HpyCH4III ACNGT 4 cut(s) 499, 959, 1106, 1175
HpyCH4IV ACGT 2 cut(s) 376, 477
HpyCH4V TGCA 7 cut(s) 545, 715, 746, 1017, 1142, 1244, 1284
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 200, 749, 1237
HpyF3I CTNAG 3 cut(s) 336, 510, 1129
HpySE526I ACGT 2 cut(s) 376, 477
Hsp92II CATG 3 cut(s) 734, 1052, 1223
HspAI GCGC 3 cut(s) 49, 242, 752
KspAI GTTAAC 1 cut(s) 859
Kzo9I GATC 4 cut(s) 302, 649, 733, 1125
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 1 cut(s) 602
Lsp1109I GCAGC 5 cut(s) 58, 103, 139, 730, 1366
LweI GCATC 4 cut(s) 532, 619, 730, 1312
MaeI CTAG 3 cut(s) 245, 1041, 1295
MaeII ACGT 2 cut(s) 376, 477
MaeIII GTNAC 4 cut(s) 412, 445, 473, 721
MalI GATC 4 cut(s) 304, 651, 735, 1127
MboI GATC 4 cut(s) 302, 649, 733, 1125
MboII GAAGA 9 cut(s) 32, 111, 196, 199, 443, 674, 1124, 1323, 1337
MflI RGATCY 1 cut(s) 1125
MhlI GDGCHC 3 cut(s) 205, 974, 1144
MluCI AATT 5 cut(s) 352, 819, 1011, 1053, 1134
MmeI TCCRAC 3 cut(s) 280, 504, 1050
Mph1103I ATGCAT 1 cut(s) 547
MroXI GAANNNNTTC 1 cut(s) 24
MseI TTAA 8 cut(s) 327, 344, 491, 501, 552, 609, 858, 1214
MspI CCGG 3 cut(s) 450, 862, 1075
MspR9I CCNGG 2 cut(s) 225, 897
MvaI CCWGG 2 cut(s) 225, 897
MvnI CGCG 1 cut(s) 754
MwoI GCNNNNNNNGC 4 cut(s) 149, 200, 749, 1237
NdeI CATATG 1 cut(s) 924
NdeII GATC 4 cut(s) 302, 649, 733, 1125
NheI GCTAGC 1 cut(s) 244
NlaIII CATG 3 cut(s) 734, 1052, 1223
NlaIV GGNNCC 2 cut(s) 282, 528
NmeAIII GCCGAG 1 cut(s) 831
NmuCI GTSAC 2 cut(s) 412, 473
NsiI ATGCAT 1 cut(s) 547
NspI RCATGY 1 cut(s) 1223
PaeI GCATGC 1 cut(s) 1223
PceI AGGCCT 1 cut(s) 78
PciSI GCTCTTC 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 24
PfoI TCCNGGA 1 cut(s) 223
PinAI ACCGGT 3 cut(s) 449, 861, 1074
PkrI GCNGC 6 cut(s) 48, 118, 154, 745, 756, 1356
Ppu21I YACGTR 1 cut(s) 377
Psp124BI GAGCTC 1 cut(s) 974
Psp6I CCWGG 2 cut(s) 223, 895
PspGI CCWGG 2 cut(s) 223, 895
PspN4I GGNNCC 2 cut(s) 282, 528
PspPI GGNCC 3 cut(s) 218, 526, 1072
PsuI RGATCY 1 cut(s) 1125
RsaI GTAC 4 cut(s) 672, 921, 1309, 1367
RsaNI GTAC 4 cut(s) 671, 920, 1308, 1366
SacI GAGCTC 1 cut(s) 974
SalI GTCGAC 1 cut(s) 31
SapI GCTCTTC 1 cut(s) 94
SaqAI TTAA 8 cut(s) 327, 344, 491, 501, 552, 609, 858, 1214
SatI GCNGC 6 cut(s) 47, 117, 153, 744, 755, 1355
Sau3AI GATC 4 cut(s) 302, 649, 733, 1125
Sau96I GGNCC 3 cut(s) 218, 526, 1072
ScrFI CCNGG 2 cut(s) 225, 897
SduI GDGCHC 3 cut(s) 205, 974, 1144
SfaNI GCATC 4 cut(s) 532, 619, 730, 1312
SfcI CTRYAG 2 cut(s) 639, 1161
SinI GGWCC 3 cut(s) 218, 526, 1072
SmlI CTYRAG 3 cut(s) 86, 261, 759
SmoI CTYRAG 3 cut(s) 86, 261, 759
SnaBI TACGTA 1 cut(s) 377
SphI GCATGC 1 cut(s) 1223
Sse9I AATT 5 cut(s) 352, 819, 1011, 1053, 1134
SseBI AGGCCT 1 cut(s) 78
SsiI CCGC 3 cut(s) 600, 754, 884
SspI AATATT 1 cut(s) 993
SspMI CTAG 3 cut(s) 245, 1041, 1295
SstI GAGCTC 1 cut(s) 974
StuI AGGCCT 1 cut(s) 78
StyD4I CCNGG 2 cut(s) 223, 895
StyI CCWWGG 2 cut(s) 79, 110
TaaI ACNGT 4 cut(s) 499, 959, 1106, 1175
TaiI ACGT 2 cut(s) 379, 480
TaqI TCGA 1 cut(s) 32
TasI AATT 5 cut(s) 352, 819, 1011, 1053, 1134
TatI WGTACW 1 cut(s) 1365
TauI GCSGC 1 cut(s) 757
Tru1I TTAA 8 cut(s) 327, 344, 491, 501, 552, 609, 858, 1214
Tru9I TTAA 8 cut(s) 327, 344, 491, 501, 552, 609, 858, 1214
TseFI GTSAC 2 cut(s) 412, 473
TseI GCWGC 5 cut(s) 46, 116, 152, 743, 1354
Tsp45I GTSAC 2 cut(s) 412, 473
TspDTI ATGAA 6 cut(s) 417, 815, 888, 898, 936, 1277
TspGWI ACGGA 2 cut(s) 273, 431
VneI GTGCAC 1 cut(s) 1140
VpaK11BI GGWCC 3 cut(s) 218, 526, 1072
XagI CCTNNNNNAGG 2 cut(s) 228, 1298
XapI RAATTY 3 cut(s) 352, 1011, 1053
XceI RCATGY 1 cut(s) 1223
XmiI GTMKAC 3 cut(s) 32, 312, 373
XmnI GAANNNNTTC 1 cut(s) 24
XspI CTAG 3 cut(s) 245, 1041, 1295
Zsp2I ATGCAT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.