Rh2CG256000

Belongs to the RuvB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002367
Physical Location & Seq
Reverse (-)
27278393 .. 27295779
17387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG256000.1

Sequence Viewer

Length: 627 bp
ATGATTGTGGCTGATTTTTACGTTATGGTGAGTGTCGCGAACCGGGTTCCGACCGGACTTATTGCAGCCTGCCAAGGAAAGATAGAGGAATTTGGTGGTCCTCTACGTACGACTATACTTGATCTGAGGAACATCTTGACATTTTTCAGTGAAGACTATAACAAGAATGATCAACCCCAAAACGAGGCTCCCCTCATCAATGTGATTCCAATCAGATTTGCTATAAGAGGTTTCTCTGCCAATCCAGAGAAAAGAGATAGGAAGAGGGACAAGGTGAAAATAGAAGAGGTTCAGTCCACTACCAAGAAGCAGCGCATAGCCACTCACACCCACATCAAAGGCCTTGGTCTTGAGGCCAATGGAAGAGCAGCACCTTGGGCTGCTGGCTTTGTGGGTCAGGGGGAGGCCAGAGAAGCTGTTGGTCTTCTTGTTGATATGATACGGCAAAAGAAGATGGCTGGTAAGGCACTTCTGATGGTTGGACCTCCTGGAACTGGAAAGACAGCACTAGCTCTTGGAATATCCCAGGAGCTTGGCAGTAAGGTTCCATTCTGCCCGATGGTTGGATCTGAAGTATACTCATCAAAAGTTAAGAAAACTGAGGTTTTAATGGAAAATTTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000491 GO:0000492 GO:0000785 GO:0000790 GO:0000812 GO:0002682 GO:0002831 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009888 GO:0009889 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010604 GO:0010628 GO:0010755 GO:0010756 GO:0010941 GO:0010954 GO:0016043 GO:0016363 GO:0016462 GO:0016569 GO:0016570 GO:0016573 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0030162 GO:0031011 GO:0031248 GO:0031323 GO:0031325 GO:0031326 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032270 GO:0032392 GO:0032502 GO:0032508 GO:0032991 GO:0033202 GO:0034399 GO:0034622 GO:0034708 GO:0035097 GO:0035267 GO:0036211 GO:0042623 GO:0042981 GO:0043067 GO:0043138 GO:0043140 GO:0043170 GO:0043189 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043900 GO:0043933 GO:0043967 GO:0043968 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044665 GO:0045088 GO:0045862 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048856 GO:0050776 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070035 GO:0070603 GO:0070613 GO:0071103 GO:0071339 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0080134 GO:0097255 GO:0097346 GO:0140097 GO:1900150 GO:1901564 GO:1902493 GO:1902494 GO:1902562 GO:1903317 GO:1903319 GO:1903506 GO:1904949 GO:1990234 GO:2000072 GO:2000112 GO:2000269 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
TIP49

Protein Analysis

208

Amino Acids

22.77

Weight (kDa)

9.66

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIP49 PF06068 105 - 208 9.6e-45 TIP49 P-loop domain
RuvB_N PF05496 129 - 182 1.2e-07 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 157 - 202 8.6e-07 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 576
AccII CGCG 1 cut(s) 38
AclWI GGATC 1 cut(s) 574
AcsI RAATTY 2 cut(s) 89, 616
AcuI CTGAAG 1 cut(s) 591
AfaI GTAC 1 cut(s) 109
AfiI CCNNNNNNNGG 3 cut(s) 184, 494, 563
AjnI CCWGG 2 cut(s) 487, 525
AluBI AGCT 3 cut(s) 416, 512, 532
AluI AGCT 3 cut(s) 416, 512, 532
AlwI GGATC 1 cut(s) 574
AoxI GGCC 3 cut(s) 340, 354, 405
ApeKI GCWGC 4 cut(s) 65, 310, 368, 380
ApoI RAATTY 2 cut(s) 89, 616
Asp700I GAANNNNTTC 1 cut(s) 288
AspLEI GCGC 1 cut(s) 315
AspS9I GGNCC 2 cut(s) 98, 482
AsuC2I CCSGG 1 cut(s) 44
AsuHPI GGTGA 2 cut(s) 40, 286
AvaII GGWCC 2 cut(s) 98, 482
BbsI GAAGAC 2 cut(s) 159, 416
BbvI GCAGC 4 cut(s) 77, 322, 367, 380
BccI CCATC 3 cut(s) 448, 469, 553
BceAI ACGGC 1 cut(s) 458
BciT130I CCWGG 2 cut(s) 489, 527
BclI TGATCA 1 cut(s) 169
BcnI CCSGG 1 cut(s) 44
BfaI CTAG 1 cut(s) 509
BisI GCNGC 4 cut(s) 66, 311, 369, 381
BlsI GCNGC 4 cut(s) 67, 312, 370, 382
Bme1390I CCNGG 3 cut(s) 44, 489, 527
Bme18I GGWCC 2 cut(s) 98, 482
BmgT120I GGNCC 2 cut(s) 98, 482
BmiI GGNNCC 3 cut(s) 48, 189, 546
BmrFI CCNGG 3 cut(s) 44, 489, 527
BpiI GAAGAC 2 cut(s) 159, 416
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 44
BsaAI YACGTR 1 cut(s) 107
BsaBI GATNNNNATC 1 cut(s) 209
BsaJI CCNNGG 4 cut(s) 73, 343, 374, 525
BsaWI WCCGGW 1 cut(s) 53
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 494, 563
Bse1I ACTGG 1 cut(s) 499
Bse8I GATNNNNATC 1 cut(s) 209
BseBI CCWGG 2 cut(s) 489, 527
BseDI CCNNGG 4 cut(s) 73, 343, 374, 525
BseJI GATNNNNATC 1 cut(s) 209
BseLI CCNNNNNNNGG 3 cut(s) 184, 494, 563
BseMII CTCAG 2 cut(s) 116, 591
BseNI ACTGG 1 cut(s) 499
BseXI GCAGC 4 cut(s) 77, 322, 367, 380
Bsh1236I CGCG 1 cut(s) 38
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 3 cut(s) 342, 356, 407
BsiEI CGRYCG 1 cut(s) 54
BsiSI CCGG 2 cut(s) 43, 54
BsiWI CGTACG 1 cut(s) 107
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 3 cut(s) 184, 494, 563
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 3 cut(s) 342, 356, 407
Bsp143I GATC 3 cut(s) 121, 169, 566
Bsp68I TCGCGA 1 cut(s) 38
BspANI GGCC 3 cut(s) 342, 356, 407
BspCNI CTCAG 2 cut(s) 117, 592
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 3 cut(s) 48, 189, 546
BspPI GGATC 1 cut(s) 574
BspQI GCTCTTC 1 cut(s) 358
BsrI ACTGG 1 cut(s) 499
BssECI CCNNGG 4 cut(s) 73, 343, 374, 525
BssMI GATC 3 cut(s) 121, 169, 566
BssNAI GTATAC 1 cut(s) 577
BssT1I CCWWGG 3 cut(s) 73, 343, 374
Bst1107I GTATAC 1 cut(s) 577
Bst2UI CCWGG 2 cut(s) 489, 527
Bst6I CTCTTC 3 cut(s) 257, 279, 358
BstBAI YACGTR 1 cut(s) 107
BstC8I GCNNGC 2 cut(s) 70, 385
BstDEI CTNAG 2 cut(s) 125, 600
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 3 cut(s) 124, 172, 569
BstMBI GATC 3 cut(s) 121, 169, 566
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 3 cut(s) 377, 413, 464
BstNI CCWGG 2 cut(s) 489, 527
BstSCI CCNGG 3 cut(s) 42, 487, 525
BstSNI TACGTA 1 cut(s) 107
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 4 cut(s) 77, 322, 367, 380
BstV2I GAAGAC 2 cut(s) 159, 416
BstX2I RGATCY 1 cut(s) 566
BstXI CCANNNNNNTGG 1 cut(s) 533
BstYI RGATCY 1 cut(s) 566
BstZ17I GTATAC 1 cut(s) 577
BsuRI GGCC 3 cut(s) 342, 356, 407
BtsIMutI CAGTG 1 cut(s) 154
BtuMI TCGCGA 1 cut(s) 38
Cac8I GCNNGC 2 cut(s) 70, 385
CfoI GCGC 1 cut(s) 315
Cfr13I GGNCC 2 cut(s) 98, 482
Csp6I GTAC 1 cut(s) 108
CviQI GTAC 1 cut(s) 108
DdeI CTNAG 2 cut(s) 125, 600
DpnI GATC 3 cut(s) 123, 171, 568
DpnII GATC 3 cut(s) 121, 169, 566
Eam1104I CTCTTC 3 cut(s) 257, 279, 358
EarI CTCTTC 3 cut(s) 257, 279, 358
Eco105I TACGTA 1 cut(s) 107
Eco130I CCWWGG 3 cut(s) 73, 343, 374
Eco147I AGGCCT 1 cut(s) 342
Eco47I GGWCC 2 cut(s) 98, 482
Eco57I CTGAAG 1 cut(s) 591
EcoRII CCWGG 2 cut(s) 487, 525
EcoT14I CCWWGG 3 cut(s) 73, 343, 374
ErhI CCWWGG 3 cut(s) 73, 343, 374
FaiI YATR 7 cut(s) 26, 116, 159, 224, 317, 437, 577
FaqI GGGAC 1 cut(s) 281
FbaI TGATCA 1 cut(s) 169
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 4 cut(s) 66, 311, 369, 381
Fsp4HI GCNGC 4 cut(s) 66, 311, 369, 381
FspBI CTAG 1 cut(s) 509
GlaI GCGC 1 cut(s) 314
GluI GCNGC 4 cut(s) 66, 311, 369, 381
HaeIII GGCC 3 cut(s) 342, 356, 407
HapII CCGG 2 cut(s) 43, 54
HhaI GCGC 1 cut(s) 315
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HinfI GANTC 1 cut(s) 205
HpaII CCGG 2 cut(s) 43, 54
HphI GGTGA 2 cut(s) 40, 286
Hpy166II GTNNAC 2 cut(s) 297, 577
Hpy188I TCNGA 5 cut(s) 51, 126, 215, 474, 571
Hpy188III TCNNGA 4 cut(s) 37, 136, 245, 350
Hpy8I GTNNAC 2 cut(s) 297, 577
HpyCH4IV ACGT 2 cut(s) 21, 106
HpyCH4V TGCA 1 cut(s) 65
HpyF10VI GCNNNNNNNGC 3 cut(s) 377, 413, 464
HpyF3I CTNAG 2 cut(s) 125, 600
HpySE526I ACGT 2 cut(s) 21, 106
HspAI GCGC 1 cut(s) 313
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 3 cut(s) 121, 169, 566
LguI GCTCTTC 1 cut(s) 358
LmnI GCTCC 2 cut(s) 193, 529
Lsp1109I GCAGC 4 cut(s) 77, 322, 367, 380
MaeI CTAG 1 cut(s) 509
MaeII ACGT 2 cut(s) 21, 106
MalI GATC 3 cut(s) 123, 171, 568
MboI GATC 3 cut(s) 121, 169, 566
MboII GAAGA 6 cut(s) 164, 274, 296, 375, 416, 463
MflI RGATCY 1 cut(s) 566
MluCI AATT 2 cut(s) 89, 616
MmeI TCCRAC 3 cut(s) 74, 460, 544
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 2 cut(s) 591, 608
MslI CAYNNNNRTG 1 cut(s) 200
MspI CCGG 2 cut(s) 43, 54
MspR9I CCNGG 3 cut(s) 44, 489, 527
MvaI CCWGG 2 cut(s) 489, 527
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 377, 413, 464
NciI CCSGG 1 cut(s) 44
NdeII GATC 3 cut(s) 121, 169, 566
NlaIV GGNNCC 3 cut(s) 48, 189, 546
NruI TCGCGA 1 cut(s) 38
PceI AGGCCT 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 358
PdmI GAANNNNTTC 1 cut(s) 288
PfeI GAWTC 1 cut(s) 205
Pfl23II CGTACG 1 cut(s) 107
PfoI TCCNGGA 1 cut(s) 487
PkrI GCNGC 4 cut(s) 67, 312, 370, 382
Ppu21I YACGTR 1 cut(s) 107
Psp6I CCWGG 2 cut(s) 487, 525
PspGI CCWGG 2 cut(s) 487, 525
PspLI CGTACG 1 cut(s) 107
PspN4I GGNNCC 3 cut(s) 48, 189, 546
PspPI GGNCC 2 cut(s) 98, 482
PsuI RGATCY 1 cut(s) 566
RruI TCGCGA 1 cut(s) 38
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
RseI CAYNNNNRTG 1 cut(s) 200
SapI GCTCTTC 1 cut(s) 358
SaqAI TTAA 2 cut(s) 591, 608
SatI GCNGC 4 cut(s) 66, 311, 369, 381
Sau3AI GATC 3 cut(s) 121, 169, 566
Sau96I GGNCC 2 cut(s) 98, 482
ScrFI CCNGG 3 cut(s) 44, 489, 527
SinI GGWCC 2 cut(s) 98, 482
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
SnaBI TACGTA 1 cut(s) 107
Sse9I AATT 2 cut(s) 89, 616
SseBI AGGCCT 1 cut(s) 342
SspMI CTAG 1 cut(s) 509
StuI AGGCCT 1 cut(s) 342
StyD4I CCNGG 3 cut(s) 42, 487, 525
StyI CCWWGG 3 cut(s) 73, 343, 374
TaiI ACGT 2 cut(s) 24, 109
TasI AATT 2 cut(s) 89, 616
TfiI GAWTC 1 cut(s) 205
Tru1I TTAA 2 cut(s) 591, 608
Tru9I TTAA 2 cut(s) 591, 608
TscAI CASTG 1 cut(s) 154
TseI GCWGC 4 cut(s) 65, 310, 368, 380
TspRI CASTG 1 cut(s) 154
VpaK11BI GGWCC 2 cut(s) 98, 482
XapI RAATTY 2 cut(s) 89, 616
XmiI GTMKAC 1 cut(s) 576
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.