pycom03g10400

Belongs to the RuvB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002367
Physical Location & Seq
Forward (+)
9563591 .. 9566249
2659 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g10400.6

Sequence Viewer

Length: 942 bp
ATGCCCGGCCCAAAATTCATATCTGAAATTTTAGCCCATTTAATAAGAGAGGAAGAGGAAAATAACGTCGCCTTCTTCTTCTTCTTCCTCGTGTCCGATTACACAGGGCCAACTTCTTCTTCTTCACAGGTCACAACAGCTGAACTGGAACTCTCTTCTCTAAAAACCCTAATTCAAGAGCGAGAGAGAGAGAGAGAGGGAGAGGGAGAGATGGACAAGGTGAGGATAGAAGAGGTTCAGTCGACTACCAAGAAGCAGCGCATAGCCACTCACACCCACATCAAAGGCCTTGGTCTTGAGGCCAATGGAAGGGCAATAGCGTGGGCTGCTGGCTTTGTGGGTCAGGGGGAAGCAAGAGAAGCTTCTGGCCTTGTTGTTGATATGATACGGCAGAAGAAGATGGCTGGTCGGGCACTTCTACTTGCTGGACCTCCTGGAACAGGAAAGACAGCGCTGGCTCTTGGAATATCTCAGGAGCTTGGGAGTAAGGTTCCGTTCTGCCCGATGGTTGGATCAGAAGTATACTCATCAGAAGTTAAGAAAACTGAGGTTTTAATGGAAAATTTTAGACGGGCTATTGGTCTACGTATCAAGGAAAATAAAGAGGTGTATGAAGGAGAGGTGACGGAACTCACACCAGAAGAAACAGAGAGTGTAACCGGTGGTTATGGTAAAAGCATTAGTCACGTCATCATTGGATTAAAAACTGTTAAAGGAACCAAGCAACTGAAGTTGGACCCCACTATTTATGATGCATTGATTAAGGAAAAGGTAGCTGTTGGTGATGTTATATACATTGAAGCAAATAGCGGAGCAGTTAAAAGAGTTGGTAGAAGTGATGCTTTTGCTACAGAGTTTGATCGATCATCGTGGAAGCAGAAGAGTATGTACCACTTCCAAAAGGAGAGGTTCACAAAAAGAAGGAGATTGTGCAGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000491 GO:0000492 GO:0000785 GO:0000790 GO:0000812 GO:0002682 GO:0002831 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009888 GO:0009889 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010604 GO:0010628 GO:0010755 GO:0010756 GO:0010941 GO:0010954 GO:0016043 GO:0016363 GO:0016462 GO:0016569 GO:0016570 GO:0016573 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0030162 GO:0031011 GO:0031248 GO:0031323 GO:0031325 GO:0031326 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032270 GO:0032392 GO:0032502 GO:0032508 GO:0032991 GO:0033202 GO:0034399 GO:0034622 GO:0034708 GO:0035097 GO:0035267 GO:0036211 GO:0042623 GO:0042981 GO:0043067 GO:0043138 GO:0043140 GO:0043170 GO:0043189 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043900 GO:0043933 GO:0043967 GO:0043968 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044665 GO:0045088 GO:0045862 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048856 GO:0050776 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070035 GO:0070603 GO:0070613 GO:0071103 GO:0071339 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0080134 GO:0097255 GO:0097346 GO:0140097 GO:1900150 GO:1901564 GO:1902493 GO:1902494 GO:1902562 GO:1903317 GO:1903319 GO:1903506 GO:1904949 GO:1990234 GO:2000072 GO:2000112 GO:2000269 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
TIP49

Protein Analysis

314

Amino Acids

34.78

Weight (kDa)

8.85

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 242, 522, 583
AciI CCGC 1 cut(s) 810
AclWI GGATC 1 cut(s) 520
AcsI RAATTY 3 cut(s) 14, 27, 562
AcuI CTGAAG 1 cut(s) 749
AfaI GTAC 1 cut(s) 890
AfeI AGCGCT 1 cut(s) 453
AfiI CCNNNNNNNGG 3 cut(s) 309, 440, 509
AgeI ACCGGT 1 cut(s) 659
AgsI TTSAA 2 cut(s) 176, 800
AjiI CACGTC 1 cut(s) 688
AjnI CCWGG 1 cut(s) 433
AjuI GAANNNNNNNTTGG 2 cut(s) 103, 135
AluBI AGCT 4 cut(s) 140, 362, 478, 776
AluI AGCT 4 cut(s) 140, 362, 478, 776
AlwI GGATC 1 cut(s) 520
Aor51HI AGCGCT 1 cut(s) 453
AoxI GGCC 5 cut(s) 7, 107, 286, 300, 367
ApeKI GCWGC 2 cut(s) 256, 326
ApoI RAATTY 3 cut(s) 14, 27, 562
AsiGI ACCGGT 1 cut(s) 659
Asp700I GAANNNNTTC 1 cut(s) 234
AspLEI GCGC 2 cut(s) 261, 454
AspS9I GGNCC 4 cut(s) 8, 107, 428, 736
AsuC2I CCSGG 1 cut(s) 6
AsuHPI GGTGA 3 cut(s) 232, 634, 794
AvaII GGWCC 2 cut(s) 428, 736
BaeGI GKGCMC 1 cut(s) 415
BarI GAAGNNNNNNTAC 2 cut(s) 872, 904
BauI CACGAG 1 cut(s) 89
BbvI GCAGC 2 cut(s) 268, 313
BccI CCATC 3 cut(s) 205, 394, 499
BceAI ACGGC 1 cut(s) 404
BciT130I CCWGG 1 cut(s) 435
BcnI CCSGG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 849
BfoI RGCGCY 1 cut(s) 455
BisI GCNGC 2 cut(s) 257, 327
BlsI GCNGC 2 cut(s) 258, 328
Bme1390I CCNGG 2 cut(s) 6, 435
Bme18I GGWCC 2 cut(s) 428, 736
BmgBI CACGTC 1 cut(s) 688
BmgT120I GGNCC 4 cut(s) 8, 107, 428, 736
BmiI GGNNCC 3 cut(s) 492, 718, 738
BmrFI CCNGG 2 cut(s) 6, 435
BmsI GCATC 2 cut(s) 742, 829
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 6
Bsa29I ATCGAT 1 cut(s) 862
BsaAI YACGTR 1 cut(s) 587
BsaJI CCNNGG 1 cut(s) 289
BsaWI WCCGGW 1 cut(s) 659
Bsc4I CCNNNNNNNGG 3 cut(s) 309, 440, 509
Bse118I RCCGGY 1 cut(s) 659
Bse1I ACTGG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 435
BseCI ATCGAT 1 cut(s) 862
BseDI CCNNGG 1 cut(s) 289
BseGI GGATG 1 cut(s) 942
BseLI CCNNNNNNNGG 3 cut(s) 309, 440, 509
BseMII CTCAG 2 cut(s) 485, 537
BseNI ACTGG 1 cut(s) 150
BseSI GKGCMC 1 cut(s) 415
BseXI GCAGC 2 cut(s) 268, 313
BshFI GGCC 5 cut(s) 9, 109, 288, 302, 369
BshTI ACCGGT 1 cut(s) 659
BshVI ATCGAT 1 cut(s) 862
BsiSI CCGG 2 cut(s) 6, 660
BslI CCNNNNNNNGG 3 cut(s) 309, 440, 509
BsnI GGCC 5 cut(s) 9, 109, 288, 302, 369
Bsp1286I GDGCHC 1 cut(s) 415
Bsp143I GATC 3 cut(s) 512, 859, 863
BspACI CCGC 1 cut(s) 810
BspANI GGCC 5 cut(s) 9, 109, 288, 302, 369
BspCNI CTCAG 2 cut(s) 484, 538
BspDI ATCGAT 1 cut(s) 862
BspLI GGNNCC 3 cut(s) 492, 718, 738
BspPI GGATC 1 cut(s) 520
BsrFI RCCGGY 1 cut(s) 659
BsrI ACTGG 1 cut(s) 150
BssAI RCCGGY 1 cut(s) 659
BssECI CCNNGG 1 cut(s) 289
BssMI GATC 3 cut(s) 512, 859, 863
BssNAI GTATAC 1 cut(s) 523
BssSI CACGAG 1 cut(s) 89
BssT1I CCWWGG 1 cut(s) 289
Bst1107I GTATAC 1 cut(s) 523
Bst2BI CACGAG 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 1 cut(s) 709
Bst6I CTCTTC 4 cut(s) 48, 160, 225, 875
BstBAI YACGTR 1 cut(s) 587
BstC8I GCNNGC 2 cut(s) 331, 456
BstDEI CTNAG 2 cut(s) 471, 546
BstENI CCTNNNNNAGG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 942
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 2 cut(s) 261, 454
BstKTI GATC 3 cut(s) 515, 862, 866
BstMBI GATC 3 cut(s) 512, 859, 863
BstMWI GCNNNNNNNGC 3 cut(s) 326, 359, 410
BstNI CCWGG 1 cut(s) 435
BstSCI CCNGG 2 cut(s) 4, 433
BstSFI CTRYAG 1 cut(s) 849
BstSLI GKGCMC 1 cut(s) 415
BstSNI TACGTA 1 cut(s) 587
BstV1I GCAGC 2 cut(s) 268, 313
BstZ17I GTATAC 1 cut(s) 523
Bsu15I ATCGAT 1 cut(s) 862
BsuRI GGCC 5 cut(s) 9, 109, 288, 302, 369
BsuTUI ATCGAT 1 cut(s) 862
BtrI CACGTC 1 cut(s) 688
BtsCI GGATG 1 cut(s) 942
Cac8I GCNNGC 2 cut(s) 331, 456
CfoI GCGC 2 cut(s) 261, 454
Cfr10I RCCGGY 1 cut(s) 659
Cfr13I GGNCC 4 cut(s) 8, 107, 428, 736
ClaI ATCGAT 1 cut(s) 862
Csp6I GTAC 1 cut(s) 889
CspAI ACCGGT 1 cut(s) 659
CviQI GTAC 1 cut(s) 889
DdeI CTNAG 2 cut(s) 471, 546
DpnI GATC 3 cut(s) 514, 861, 865
DpnII GATC 3 cut(s) 512, 859, 863
Eam1104I CTCTTC 4 cut(s) 48, 160, 225, 875
EarI CTCTTC 4 cut(s) 48, 160, 225, 875
Eco105I TACGTA 1 cut(s) 587
Eco130I CCWWGG 1 cut(s) 289
Eco147I AGGCCT 1 cut(s) 288
Eco47I GGWCC 2 cut(s) 428, 736
Eco47III AGCGCT 1 cut(s) 453
Eco57I CTGAAG 1 cut(s) 749
EcoNI CCTNNNNNAGG 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 289
EcoT22I ATGCAT 1 cut(s) 757
ErhI CCWWGG 1 cut(s) 289
FalI AAGNNNNNCTT 4 cut(s) 346, 378, 826, 858
FblI GTMKAC 3 cut(s) 242, 522, 583
Fnu4HI GCNGC 2 cut(s) 257, 327
Fsp4HI GCNGC 2 cut(s) 257, 327
GlaI GCGC 2 cut(s) 260, 453
GluI GCNGC 2 cut(s) 257, 327
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 5 cut(s) 9, 109, 288, 302, 369
HapII CCGG 2 cut(s) 6, 660
HhaI GCGC 2 cut(s) 261, 454
Hin6I GCGC 2 cut(s) 259, 452
HinP1I GCGC 2 cut(s) 259, 452
HincII GTYRAC 1 cut(s) 243
HindII GTYRAC 1 cut(s) 243
HindIII AAGCTT 1 cut(s) 360
HpaII CCGG 2 cut(s) 6, 660
HphI GGTGA 3 cut(s) 232, 634, 794
Hpy166II GTNNAC 4 cut(s) 243, 523, 584, 912
Hpy188I TCNGA 4 cut(s) 25, 97, 517, 532
Hpy188III TCNNGA 3 cut(s) 176, 296, 473
Hpy8I GTNNAC 4 cut(s) 243, 523, 584, 912
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 4 cut(s) 82, 303, 608, 915
HpyCH4III ACNGT 1 cut(s) 709
HpyCH4IV ACGT 3 cut(s) 66, 586, 687
HpyCH4V TGCA 2 cut(s) 755, 933
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 359, 410
HpyF3I CTNAG 2 cut(s) 471, 546
HpySE526I ACGT 3 cut(s) 66, 586, 687
HspAI GCGC 2 cut(s) 259, 452
Kzo9I GATC 3 cut(s) 512, 859, 863
LmnI GCTCC 2 cut(s) 475, 812
Lsp1109I GCAGC 2 cut(s) 268, 313
LweI GCATC 2 cut(s) 742, 829
MaeII ACGT 3 cut(s) 66, 586, 687
MaeIII GTNAC 4 cut(s) 130, 622, 655, 683
MalI GATC 3 cut(s) 514, 861, 865
MboI GATC 3 cut(s) 512, 859, 863
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 4 cut(s) 14, 27, 171, 562
MmeI TCCRAC 2 cut(s) 490, 714
Mph1103I ATGCAT 1 cut(s) 757
MroXI GAANNNNTTC 1 cut(s) 234
MseI TTAA 7 cut(s) 41, 537, 554, 701, 711, 762, 819
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 2 cut(s) 6, 660
MspR9I CCNGG 2 cut(s) 6, 435
MvaI CCWGG 1 cut(s) 435
MwoI GCNNNNNNNGC 3 cut(s) 326, 359, 410
NciI CCSGG 1 cut(s) 6
NdeII GATC 3 cut(s) 512, 859, 863
NlaIV GGNNCC 3 cut(s) 492, 718, 738
NmuCI GTSAC 3 cut(s) 130, 622, 683
NsiI ATGCAT 1 cut(s) 757
PceI AGGCCT 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 234
PfoI TCCNGGA 1 cut(s) 433
PinAI ACCGGT 1 cut(s) 659
PkrI GCNGC 2 cut(s) 258, 328
Ppu21I YACGTR 1 cut(s) 587
Psp6I CCWGG 1 cut(s) 433
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 3 cut(s) 492, 718, 738
PspPI GGNCC 4 cut(s) 8, 107, 428, 736
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 1 cut(s) 890
RsaNI GTAC 1 cut(s) 889
SalI GTCGAC 1 cut(s) 241
SaqAI TTAA 7 cut(s) 41, 537, 554, 701, 711, 762, 819
SatI GCNGC 2 cut(s) 257, 327
Sau3AI GATC 3 cut(s) 512, 859, 863
Sau96I GGNCC 4 cut(s) 8, 107, 428, 736
ScrFI CCNGG 2 cut(s) 6, 435
SduI GDGCHC 1 cut(s) 415
SfaNI GCATC 2 cut(s) 742, 829
SfcI CTRYAG 1 cut(s) 849
SinI GGWCC 2 cut(s) 428, 736
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SnaBI TACGTA 1 cut(s) 587
Sse9I AATT 4 cut(s) 14, 27, 171, 562
SseBI AGGCCT 1 cut(s) 288
SsiI CCGC 1 cut(s) 810
StuI AGGCCT 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 4, 433
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 1 cut(s) 709
TaiI ACGT 3 cut(s) 69, 589, 690
TaqI TCGA 2 cut(s) 242, 862
TasI AATT 4 cut(s) 14, 27, 171, 562
Tru1I TTAA 7 cut(s) 41, 537, 554, 701, 711, 762, 819
Tru9I TTAA 7 cut(s) 41, 537, 554, 701, 711, 762, 819
TseFI GTSAC 3 cut(s) 130, 622, 683
TseI GCWGC 2 cut(s) 256, 326
Tsp45I GTSAC 3 cut(s) 130, 622, 683
TspDTI ATGAA 2 cut(s) 7, 627
TspGWI ACGGA 2 cut(s) 483, 641
VpaK11BI GGWCC 2 cut(s) 428, 736
XagI CCTNNNNNAGG 1 cut(s) 438
XapI RAATTY 3 cut(s) 14, 27, 562
XmiI GTMKAC 3 cut(s) 242, 522, 583
XmnI GAANNNNTTC 1 cut(s) 234
Zsp2I ATGCAT 1 cut(s) 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.