AT5G23520

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006879
Physical Location & Seq
Reverse (-)
7928993 .. 7931793
2801 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23520.1

Sequence Viewer

Length: 1308 bp
ATGTCATGGATGAAAGGTAAATCATCTGGTTGGACTGCGTTTGATCTCAAGCAGAGACAGAAACAAGGTCTTGAAAGTGAGGTCGAGGGTGATCCATTCCCACCTGTTTCAACTAGTGTTAATGCATCCTTTGGTGTAAGAGGACGGTTGAGAAGAAACCATGAACCTTCTGAAAAGTCTTTTTCGTCCGTGCTCTTGCCTCCTTCGAGATTTCCGGCATTGACAGAGAATAAGGATTGCGGAAACCAAGAACGAGGGGGTTGTTGTAGAAGAAAGCCGGACACTTTGAGTCTCCCGGTAAACAGTCATGATTTAGCCTTCACGAAGCTGAAGGAAATGAACAGCTGGGCGGATGATAACTTAATAAGGGATGTATTGTTATCCACAGAGGATGATTTTGAAATGGCCTTAGCTTTTTTGAAAGGAATGGTTTCTTCTGGTAAGGAGGATGAGGAGCCAACTTCAAAGATTGAGGGGTATTCATCCGATAACAGAAGATCGGAATATAGAACATTTGAGAAAACAGTTACAAGTTCGGTGAAGATGGCTGCAAGGTCCACATTTGAGGATGCTGGCAAGTATGATCTGGAAAATAGTGATGGAAGCTCGTTTCTGGTCAATGCATCTGACAATGAAAAGTTCCCTGATGATATTTCTGAACTAGATAGTATCATTCAGCGGCTTCAGTCCATTCCGATAGAACCTGAATGGGAAGAAGATGATCTATACTTGAGTCATCGAAAGGATGCGCTGAAAGTGATGAGATCAGCCTCAAACCATTCAAGAGCTGCCCAAAATGCTTTTCAGAGATATGATCATGCTTCTGCCAAGCAGCATTCAGATAAAGCAAGAGAGGACTGGTTAGCAGCTGAGAAGCTAAATGCTGAAGCGGCCAAGAAGATCATAGGTATAACAAATAAGGATAATGACATATGGAAGTTGGACCTGCATGGTCTCCATGCCACAGAAGCTGTTCAAGCCTTACAAGAACGTTTGCAAATGATTGAAGGTCACTTTACTGTGAACCGTTCAGTATCACCAAATAGAGGTAGGTCAAAGAATGCAGCTCTGCGATCTGCATCACAAGAGCCTTTTGGTAGATTAGATGAGGAAGGCATGCACTGTCAAAGAACTTCTTCTAGAGAACTGAGAAACTCATTGCAGGTTATAACAGGTATTGGTAAACACAGTCGCGGACAGGCTTCACTTCCTCTAGCCGTGAAGACATTCTTTGAAGATAACAGGTATAGGTTTGACGAGACAAGGCCAGGAGTTATCACAGTGCGGCCTAAATTCCGACACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

435

Amino Acids

49.12

Weight (kDa)

6.49

Isoelectric Point (pI)

55.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 243 - 302 1.2e-12 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1169
Acc36I ACCTGC 2 cut(s) 954, 1153
AccII CGCG 1 cut(s) 1194
AciI CCGC 6 cut(s) 240, 350, 679, 890, 1194, 1285
AclI AACGTT 1 cut(s) 991
AclWI GGATC 1 cut(s) 86
AcoI YGGCCR 1 cut(s) 891
AcsI RAATTY 1 cut(s) 1292
AcuI CTGAAG 3 cut(s) 350, 668, 906
AfiI CCNNNNNNNGG 1 cut(s) 1046
AgsI TTSAA 9 cut(s) 74, 111, 401, 421, 465, 783, 977, 1007, 1235
AhlI ACTAGT 1 cut(s) 113
AjnI CCWGG 1 cut(s) 1267
AjuI GAANNNNNNNTTGG 2 cut(s) 786, 818
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 4 cut(s) 49, 296, 959, 1253
AlwI GGATC 1 cut(s) 86
AlwNI CAGNNNCTG 1 cut(s) 971
AoxI GGCC 4 cut(s) 405, 891, 1265, 1286
ApeKI GCWGC 5 cut(s) 548, 788, 832, 866, 1064
ApoI RAATTY 1 cut(s) 1292
Asp700I GAANNNNTTC 4 cut(s) 430, 972, 1135, 1226
AspLEI GCGC 1 cut(s) 751
AspS9I GGNCC 2 cut(s) 555, 943
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 3 cut(s) 101, 550, 1029
AvaII GGWCC 2 cut(s) 555, 943
BbsI GAAGAC 1 cut(s) 1229
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 5 cut(s) 535, 775, 844, 878, 1076
BccI CCATC 2 cut(s) 538, 593
BceAI ACGGC 1 cut(s) 1202
BciT130I CCWGG 1 cut(s) 1269
BclI TGATCA 1 cut(s) 814
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 4 cut(s) 49, 296, 959, 1253
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 4 cut(s) 114, 662, 1140, 1214
BfuAI ACCTGC 2 cut(s) 954, 1153
BisI GCNGC 8 cut(s) 549, 680, 789, 833, 867, 891, 1065, 1286
BlsI GCNGC 8 cut(s) 550, 681, 790, 834, 868, 892, 1066, 1287
Bme1390I CCNGG 2 cut(s) 296, 1269
Bme18I GGWCC 2 cut(s) 555, 943
BmgT120I GGNCC 2 cut(s) 555, 943
BmiI GGNNCC 1 cut(s) 456
BmrFI CCNGG 2 cut(s) 296, 1269
BmsI GCATC 5 cut(s) 134, 559, 632, 736, 1088
BpiI GAAGAC 1 cut(s) 1229
Bpu10I CCTNAGC 1 cut(s) 409
BpuEI CTTGAG 2 cut(s) 32, 751
BpuMI CCSGG 1 cut(s) 296
BsaBI GATNNNNATC 1 cut(s) 1078
BsaI GGTCTC 1 cut(s) 959
Bsc4I CCNNNNNNNGG 1 cut(s) 1046
Bse1I ACTGG 1 cut(s) 863
Bse3DI GCAATG 1 cut(s) 1157
Bse8I GATNNNNATC 1 cut(s) 1078
BseBI CCWGG 1 cut(s) 1269
BseGI GGATG 9 cut(s) 15, 125, 358, 376, 397, 454, 482, 574, 751
BseJI GATNNNNATC 1 cut(s) 1078
BseLI CCNNNNNNNGG 1 cut(s) 1046
BseMI GCAATG 1 cut(s) 1157
BseMII CTCAG 2 cut(s) 861, 1139
BseNI ACTGG 1 cut(s) 863
BseRI GAGGAG 1 cut(s) 467
BseXI GCAGC 5 cut(s) 535, 775, 844, 878, 1076
BseYI CCCAGC 1 cut(s) 345
Bsh1236I CGCG 1 cut(s) 1194
BshFI GGCC 4 cut(s) 407, 893, 1267, 1288
BsiHKAI GWGCWC 1 cut(s) 195
BsiSI CCGG 3 cut(s) 215, 278, 296
BslI CCNNNNNNNGG 1 cut(s) 1046
BsmAI GTCTC 4 cut(s) 49, 296, 959, 1253
BsmI GAATGC 2 cut(s) 835, 1066
BsnI GGCC 4 cut(s) 407, 893, 1267, 1288
Bso31I GGTCTC 1 cut(s) 959
Bsp1286I GDGCHC 1 cut(s) 195
Bsp143I GATC 9 cut(s) 43, 91, 497, 583, 721, 764, 814, 900, 1073
BspACI CCGC 6 cut(s) 240, 350, 679, 890, 1194, 1285
BspANI GGCC 4 cut(s) 407, 893, 1267, 1288
BspCNI CTCAG 2 cut(s) 862, 1140
BspFNI CGCG 1 cut(s) 1194
BspHI TCATGA 1 cut(s) 307
BspLI GGNNCC 1 cut(s) 456
BspMI ACCTGC 2 cut(s) 954, 1153
BspPI GGATC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 959
BsrDI GCAATG 1 cut(s) 1157
BsrI ACTGG 1 cut(s) 863
BssMI GATC 9 cut(s) 43, 91, 497, 583, 721, 764, 814, 900, 1073
Bst2UI CCWGG 1 cut(s) 1269
Bst4CI ACNGT 8 cut(s) 147, 305, 526, 1021, 1028, 1124, 1190, 1282
BstC8I GCNNGC 2 cut(s) 574, 1118
BstDEI CTNAG 3 cut(s) 409, 870, 1148
BstF5I GGATG 9 cut(s) 15, 125, 358, 376, 397, 454, 482, 574, 751
BstFNI CGCG 1 cut(s) 1194
BstHHI GCGC 1 cut(s) 751
BstKTI GATC 9 cut(s) 46, 94, 500, 586, 724, 767, 817, 903, 1076
BstMAI GTCTC 4 cut(s) 49, 296, 959, 1253
BstMBI GATC 9 cut(s) 43, 91, 497, 583, 721, 764, 814, 900, 1073
BstMWI GCNNNNNNNGC 4 cut(s) 797, 890, 968, 977
BstNI CCWGG 1 cut(s) 1269
BstNSI RCATGY 1 cut(s) 1120
BstSCI CCNGG 2 cut(s) 294, 1267
BstUI CGCG 1 cut(s) 1194
BstV1I GCAGC 5 cut(s) 535, 775, 844, 878, 1076
BstV2I GAAGAC 1 cut(s) 1229
BsuRI GGCC 4 cut(s) 407, 893, 1267, 1288
BtsCI GGATG 9 cut(s) 15, 125, 358, 376, 397, 454, 482, 574, 751
BtsIMutI CAGTG 2 cut(s) 1120, 1287
BveI ACCTGC 2 cut(s) 954, 1153
Cac8I GCNNGC 2 cut(s) 574, 1118
CaiI CAGNNNCTG 1 cut(s) 971
CciI TCATGA 1 cut(s) 307
CfoI GCGC 1 cut(s) 751
Cfr13I GGNCC 2 cut(s) 555, 943
CviAII CATG 7 cut(s) 6, 161, 308, 818, 950, 959, 1117
DdeI CTNAG 3 cut(s) 409, 870, 1148
DpnI GATC 9 cut(s) 45, 93, 499, 585, 723, 766, 816, 902, 1075
DpnII GATC 9 cut(s) 43, 91, 497, 583, 721, 764, 814, 900, 1073
EaeI YGGCCR 1 cut(s) 891
EciI GGCGGA 1 cut(s) 365
Eco31I GGTCTC 1 cut(s) 959
Eco47I GGWCC 2 cut(s) 555, 943
Eco57I CTGAAG 3 cut(s) 350, 668, 906
EcoRII CCWGG 1 cut(s) 1267
EcoT22I ATGCAT 2 cut(s) 127, 625
FaeI CATG 7 cut(s) 9, 164, 311, 821, 953, 962, 1120
FalI AAGNNNNNCTT 4 cut(s) 1120, 1152, 1214, 1246
FatI CATG 7 cut(s) 5, 160, 307, 817, 949, 958, 1116
FauNDI CATATG 1 cut(s) 932
FbaI TGATCA 1 cut(s) 814
Fnu4HI GCNGC 8 cut(s) 549, 680, 789, 833, 867, 891, 1065, 1286
FokI GGATG 9 cut(s) 22, 112, 365, 383, 404, 461, 469, 581, 758
Fsp4HI GCNGC 8 cut(s) 549, 680, 789, 833, 867, 891, 1065, 1286
FspBI CTAG 4 cut(s) 114, 662, 1140, 1214
GlaI GCGC 1 cut(s) 750
GluI GCNGC 8 cut(s) 549, 680, 789, 833, 867, 891, 1065, 1286
GsaI CCCAGC 1 cut(s) 349
HaeIII GGCC 4 cut(s) 407, 893, 1267, 1288
HapII CCGG 3 cut(s) 215, 278, 296
HhaI GCGC 1 cut(s) 751
Hin1II CATG 7 cut(s) 9, 164, 311, 821, 953, 962, 1120
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HinfI GANTC 2 cut(s) 289, 733
HpaII CCGG 3 cut(s) 215, 278, 296
HphI GGTGA 3 cut(s) 101, 550, 1029
Hpy166II GTNNAC 4 cut(s) 301, 558, 1024, 1184
Hpy188I TCNGA 9 cut(s) 172, 487, 502, 628, 658, 696, 807, 841, 1298
Hpy188III TCNNGA 7 cut(s) 71, 207, 308, 322, 587, 783, 1140
Hpy8I GTNNAC 4 cut(s) 301, 558, 1024, 1184
HpyAV CCTTC 6 cut(s) 177, 213, 325, 328, 1001, 1106
HpyCH4III ACNGT 8 cut(s) 147, 305, 526, 1021, 1028, 1124, 1190, 1282
HpyCH4IV ACGT 1 cut(s) 991
HpyCH4V TGCA 9 cut(s) 125, 551, 623, 949, 997, 1064, 1079, 1120, 1162
HpyF10VI GCNNNNNNNGC 4 cut(s) 797, 890, 968, 977
HpyF3I CTNAG 3 cut(s) 409, 870, 1148
HpySE526I ACGT 1 cut(s) 991
Hsp92II CATG 7 cut(s) 9, 164, 311, 821, 953, 962, 1120
HspAI GCGC 1 cut(s) 749
Ksp22I TGATCA 1 cut(s) 814
Kzo9I GATC 9 cut(s) 43, 91, 497, 583, 721, 764, 814, 900, 1073
LmnI GCTCC 1 cut(s) 454
Lsp1109I GCAGC 5 cut(s) 535, 775, 844, 878, 1076
LweI GCATC 5 cut(s) 134, 559, 632, 736, 1088
MaeI CTAG 4 cut(s) 114, 662, 1140, 1214
MaeII ACGT 1 cut(s) 991
MaeIII GTNAC 2 cut(s) 526, 1010
MalI GATC 9 cut(s) 45, 93, 499, 585, 723, 766, 816, 902, 1075
MboI GATC 9 cut(s) 43, 91, 497, 583, 721, 764, 814, 900, 1073
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 1 cut(s) 1292
MlyI GAGTC 2 cut(s) 298, 742
MmeI TCCRAC 2 cut(s) 11, 921
Mph1103I ATGCAT 2 cut(s) 127, 625
MroXI GAANNNNTTC 4 cut(s) 430, 972, 1135, 1226
MseI TTAA 2 cut(s) 120, 362
MspA1I CMGCKG 4 cut(s) 345, 679, 869, 1305
MspI CCGG 3 cut(s) 215, 278, 296
MspR9I CCNGG 2 cut(s) 296, 1269
Mva1269I GAATGC 2 cut(s) 835, 1066
MvaI CCWGG 1 cut(s) 1269
MvnI CGCG 1 cut(s) 1194
MwoI GCNNNNNNNGC 4 cut(s) 797, 890, 968, 977
NciI CCSGG 1 cut(s) 296
NdeI CATATG 1 cut(s) 932
NdeII GATC 9 cut(s) 43, 91, 497, 583, 721, 764, 814, 900, 1073
NlaIII CATG 7 cut(s) 9, 164, 311, 821, 953, 962, 1120
NlaIV GGNNCC 1 cut(s) 456
NmuCI GTSAC 1 cut(s) 1010
NsiI ATGCAT 2 cut(s) 127, 625
NspI RCATGY 1 cut(s) 1120
PaeI GCATGC 1 cut(s) 1120
PagI TCATGA 1 cut(s) 307
PctI GAATGC 2 cut(s) 835, 1066
PdmI GAANNNNTTC 4 cut(s) 430, 972, 1135, 1226
PkrI GCNGC 8 cut(s) 550, 681, 790, 834, 868, 892, 1066, 1287
PleI GAGTC 2 cut(s) 297, 741
PpsI GAGTC 2 cut(s) 297, 741
PsiI TTATAA 1 cut(s) 1169
Psp1406I AACGTT 1 cut(s) 991
Psp6I CCWGG 1 cut(s) 1267
PspFI CCCAGC 1 cut(s) 345
PspGI CCWGG 1 cut(s) 1267
PspN4I GGNNCC 1 cut(s) 456
PspPI GGNCC 2 cut(s) 555, 943
PstNI CAGNNNCTG 1 cut(s) 971
PvuII CAGCTG 3 cut(s) 345, 869, 1305
SaqAI TTAA 2 cut(s) 120, 362
SatI GCNGC 8 cut(s) 549, 680, 789, 833, 867, 891, 1065, 1286
Sau3AI GATC 9 cut(s) 43, 91, 497, 583, 721, 764, 814, 900, 1073
Sau96I GGNCC 2 cut(s) 555, 943
SchI GAGTC 2 cut(s) 298, 742
ScrFI CCNGG 2 cut(s) 296, 1269
SduI GDGCHC 1 cut(s) 195
SfaNI GCATC 5 cut(s) 134, 559, 632, 736, 1088
SinI GGWCC 2 cut(s) 555, 943
SmlI CTYRAG 2 cut(s) 47, 730
SmoI CTYRAG 2 cut(s) 47, 730
SpeI ACTAGT 1 cut(s) 113
SphI GCATGC 1 cut(s) 1120
Sse9I AATT 1 cut(s) 1292
SsiI CCGC 6 cut(s) 240, 350, 679, 890, 1194, 1285
SspMI CTAG 4 cut(s) 114, 662, 1140, 1214
StyD4I CCNGG 2 cut(s) 294, 1267
TaaI ACNGT 8 cut(s) 147, 305, 526, 1021, 1028, 1124, 1190, 1282
TaiI ACGT 1 cut(s) 994
TaqI TCGA 3 cut(s) 84, 206, 739
TasI AATT 1 cut(s) 1292
TauI GCSGC 3 cut(s) 682, 893, 1288
Tru1I TTAA 2 cut(s) 120, 362
Tru9I TTAA 2 cut(s) 120, 362
TscAI CASTG 2 cut(s) 1127, 1287
TseFI GTSAC 1 cut(s) 1010
TseI GCWGC 5 cut(s) 548, 788, 832, 866, 1064
Tsp45I GTSAC 1 cut(s) 1010
TspDTI ATGAA 5 cut(s) 26, 177, 353, 471, 648
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 2 cut(s) 1127, 1287
VpaK11BI GGWCC 2 cut(s) 555, 943
XapI RAATTY 1 cut(s) 1292
XbaI TCTAGA 1 cut(s) 1139
XceI RCATGY 1 cut(s) 1120
XmnI GAANNNNTTC 4 cut(s) 430, 972, 1135, 1226
XspI CTAG 4 cut(s) 114, 662, 1140, 1214
Zsp2I ATGCAT 2 cut(s) 127, 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.