Rorug02G0204600

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006879
Physical Location & Seq
Forward (+)
19185105 .. 19186075
971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0204600.1

Sequence Viewer

Length: 714 bp
ATGATGATTTCCTCTATCTTCTTCATATTTTTTCTCATTCTCTCCTCTTCCTATGCGGCTGTCCAAGATTTCTGTGTTGCAGACTATACAACTCCTCCAAGCCCTACAGGATACTCTTGCAAAAACCCCTCAGATGTTAATGTAGGCAATTTCTGTGTTGTAGACTATACAGCTCCTCCAAGCCCTGCAGGATACTCTTGCAAAAACCCCTCAGATGTTAAGGTAGACGATTTTGTGTACTCTGGCCTAGGAGTTCCTGGTAACAGCTCAAATATAAACAAATTTGGACTCAAAACTGCATTTGTTGCTCAATTTCCTGGTCTGAATGGCCTTGGTATTTCGCTGGCTCGTGGGGATTTGGAGGTTGGTGGAGTTTCTCCGATGCACTCTCACCGCGGAGCTTCAGAGACCATACTTATTGCGGAAGGCAAAGTTATATCTGGGTTCATCGCCTCGGATAACAAAGCTTATGTAAAAACACTGAAGACAGGTGATATTATGGTTTTACCTCAAGGTTTGCTTCACTTCCAAGTAAACGCAGGTGATACTCCAGCCCTTATATTTGCTAGCTTCAATAGTGACAACCCAGGTGTGCAGGTTTTGGAGACTGCACTGTTTCAAAATGATTTACGTACTGAATTGATAGCACAAACTACTCTCCTTGACACTGCTGAGATTAAGAAACTTAAGGGTCTTCTTGGTGGTACTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

237

Amino Acids

25.06

Weight (kDa)

5.08

Isoelectric Point (pI)

28.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 82 - 226 3.4e-32 Cupin
Cupin_2 PF07883 119 - 188 5.5e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 530
Acc36I ACCTGC 2 cut(s) 530, 586
AccI GTMKAC 2 cut(s) 162, 225
AccII CGCG 1 cut(s) 396
AciI CCGC 4 cut(s) 56, 394, 396, 422
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 2 cut(s) 387, 503
AfaI GTAC 3 cut(s) 239, 634, 706
AflII CTTAAG 1 cut(s) 686
AgsI TTSAA 2 cut(s) 574, 620
AjnI CCWGG 3 cut(s) 256, 316, 586
AluBI AGCT 5 cut(s) 173, 267, 401, 467, 570
AluI AGCT 5 cut(s) 173, 267, 401, 467, 570
Alw26I GTCTC 2 cut(s) 401, 599
AoxI GGCC 2 cut(s) 244, 328
ApoI RAATTY 1 cut(s) 281
AspA2I CCTAGG 1 cut(s) 247
AsuHPI GGTGA 3 cut(s) 383, 503, 554
AsuNHI GCTAGC 1 cut(s) 566
AvrII CCTAGG 1 cut(s) 247
BauI CACGAG 1 cut(s) 348
BbsI GAAGAC 2 cut(s) 491, 686
BciT130I CCWGG 3 cut(s) 258, 318, 588
BciVI GTATCC 2 cut(s) 104, 185
BcoDI GTCTC 2 cut(s) 401, 599
BfaI CTAG 2 cut(s) 248, 567
BfmI CTRYAG 2 cut(s) 105, 186
BfrI CTTAAG 1 cut(s) 686
BfuAI ACCTGC 2 cut(s) 530, 586
BfuI GTATCC 2 cut(s) 104, 185
BisI GCNGC 1 cut(s) 57
BlnI CCTAGG 1 cut(s) 247
BlsI GCNGC 1 cut(s) 58
Bme1390I CCNGG 3 cut(s) 258, 318, 588
BmrFI CCNGG 3 cut(s) 258, 318, 588
BmsI GCATC 1 cut(s) 372
BmtI GCTAGC 1 cut(s) 570
BpiI GAAGAC 2 cut(s) 491, 686
BpmI CTGGAG 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 495
BsaAI YACGTR 1 cut(s) 632
BsaI GGTCTC 1 cut(s) 401
BsaJI CCNNGG 5 cut(s) 247, 331, 394, 453, 586
BsaXI ACNNNNNCTCC 4 cut(s) 79, 109, 160, 190
BseBI CCWGG 3 cut(s) 258, 318, 588
BseDI CCNNGG 5 cut(s) 247, 331, 394, 453, 586
BseMII CTCAG 3 cut(s) 144, 225, 663
BseRI GAGGAG 3 cut(s) 34, 84, 165
BsgI GTGCAG 2 cut(s) 594, 614
Bsh1236I CGCG 1 cut(s) 396
BshFI GGCC 2 cut(s) 246, 330
BsmAI GTCTC 2 cut(s) 401, 599
BsnI GGCC 2 cut(s) 246, 330
Bso31I GGTCTC 1 cut(s) 401
BspACI CCGC 4 cut(s) 56, 394, 396, 422
BspANI GGCC 2 cut(s) 246, 330
BspCNI CTCAG 3 cut(s) 143, 224, 664
BspFNI CGCG 1 cut(s) 396
BspMAI CTGCAG 1 cut(s) 190
BspMI ACCTGC 2 cut(s) 530, 586
BspOI GCTAGC 1 cut(s) 570
BspTI CTTAAG 1 cut(s) 686
BspTNI GGTCTC 1 cut(s) 401
BssECI CCNNGG 5 cut(s) 247, 331, 394, 453, 586
BssSI CACGAG 1 cut(s) 348
BssT1I CCWWGG 2 cut(s) 247, 331
Bst2BI CACGAG 1 cut(s) 348
Bst2UI CCWGG 3 cut(s) 258, 318, 588
Bst4CI ACNGT 1 cut(s) 615
Bst6I CTCTTC 1 cut(s) 52
BstAFI CTTAAG 1 cut(s) 686
BstAPI GCANNNNNTGC 1 cut(s) 305
BstBAI YACGTR 1 cut(s) 632
BstC8I GCNNGC 2 cut(s) 345, 568
BstDEI CTNAG 3 cut(s) 130, 211, 672
BstDSI CCRYGG 1 cut(s) 394
BstFNI CGCG 1 cut(s) 396
BstMAI GTCTC 2 cut(s) 401, 599
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 3 cut(s) 258, 318, 588
BstSCI CCNGG 3 cut(s) 256, 316, 586
BstSFI CTRYAG 2 cut(s) 105, 186
BstSNI TACGTA 1 cut(s) 632
BstUI CGCG 1 cut(s) 396
BstV2I GAAGAC 2 cut(s) 491, 686
BsuI GTATCC 2 cut(s) 104, 185
BsuRI GGCC 2 cut(s) 246, 330
BtgI CCRYGG 1 cut(s) 394
BtgZI GCGATG 1 cut(s) 433
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 3 cut(s) 479, 611, 666
BveI ACCTGC 2 cut(s) 530, 586
Cac8I GCNNGC 2 cut(s) 345, 568
Cfr42I CCGCGG 1 cut(s) 397
Csp6I GTAC 3 cut(s) 238, 633, 705
CviQI GTAC 3 cut(s) 238, 633, 705
DdeI CTNAG 3 cut(s) 130, 211, 672
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
Eco105I TACGTA 1 cut(s) 632
Eco130I CCWWGG 2 cut(s) 247, 331
Eco31I GGTCTC 1 cut(s) 401
Eco57I CTGAAG 2 cut(s) 387, 503
EcoRII CCWGG 3 cut(s) 256, 316, 586
EcoT14I CCWWGG 2 cut(s) 247, 331
ErhI CCWWGG 2 cut(s) 247, 331
FalI AAGNNNNNCTT 2 cut(s) 504, 536
FblI GTMKAC 2 cut(s) 162, 225
Fnu4HI GCNGC 1 cut(s) 57
Fsp4HI GCNGC 1 cut(s) 57
FspBI CTAG 2 cut(s) 248, 567
GluI GCNGC 1 cut(s) 57
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 2 cut(s) 246, 330
HindIII AAGCTT 1 cut(s) 465
HinfI GANTC 1 cut(s) 288
HphI GGTGA 3 cut(s) 383, 503, 554
Hpy166II GTNNAC 4 cut(s) 163, 226, 238, 535
Hpy188I TCNGA 6 cut(s) 133, 214, 324, 381, 406, 457
Hpy8I GTNNAC 4 cut(s) 163, 226, 238, 535
HpyAV CCTTC 1 cut(s) 419
HpyCH4III ACNGT 1 cut(s) 615
HpyCH4IV ACGT 1 cut(s) 631
HpyCH4V TGCA 8 cut(s) 80, 120, 188, 201, 299, 385, 595, 611
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 3 cut(s) 130, 211, 672
HpySE526I ACGT 1 cut(s) 631
KspI CCGCGG 1 cut(s) 397
LmnI GCTCC 2 cut(s) 178, 398
LweI GCATC 1 cut(s) 372
MaeI CTAG 2 cut(s) 248, 567
MaeII ACGT 1 cut(s) 631
MaeIII GTNAC 2 cut(s) 260, 578
MboII GAAGA 5 cut(s) 10, 13, 39, 496, 686
MluCI AATT 5 cut(s) 148, 281, 311, 638, 709
MlyI GAGTC 1 cut(s) 282
MnlI CCTC 9 cut(s) 22, 55, 105, 139, 186, 220, 355, 463, 519
MseI TTAA 4 cut(s) 138, 219, 678, 687
MspA1I CMGCKG 1 cut(s) 396
MspCI CTTAAG 1 cut(s) 686
MspR9I CCNGG 3 cut(s) 258, 318, 588
MvaI CCWGG 3 cut(s) 258, 318, 588
MvnI CGCG 1 cut(s) 396
MwoI GCNNNNNNNGC 1 cut(s) 305
NheI GCTAGC 1 cut(s) 566
NmuCI GTSAC 1 cut(s) 578
PaqCI CACCTGC 1 cut(s) 530
PkrI GCNGC 1 cut(s) 58
PleI GAGTC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 282
Ppu21I YACGTR 1 cut(s) 632
Psp6I CCWGG 3 cut(s) 256, 316, 586
PspGI CCWGG 3 cut(s) 256, 316, 586
PstI CTGCAG 1 cut(s) 190
RsaI GTAC 3 cut(s) 239, 634, 706
RsaNI GTAC 3 cut(s) 238, 633, 705
SacII CCGCGG 1 cut(s) 397
SaqAI TTAA 4 cut(s) 138, 219, 678, 687
SatI GCNGC 1 cut(s) 57
SbfI CCTGCAGG 1 cut(s) 190
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 3 cut(s) 258, 318, 588
SdaI CCTGCAGG 1 cut(s) 190
SfaNI GCATC 1 cut(s) 372
SfcI CTRYAG 2 cut(s) 105, 186
Sfr303I CCGCGG 1 cut(s) 397
SgrBI CCGCGG 1 cut(s) 397
SmlI CTYRAG 2 cut(s) 510, 686
SmoI CTYRAG 2 cut(s) 510, 686
SnaBI TACGTA 1 cut(s) 632
Sse8387I CCTGCAGG 1 cut(s) 190
Sse9I AATT 5 cut(s) 148, 281, 311, 638, 709
SsiI CCGC 4 cut(s) 56, 394, 396, 422
SspMI CTAG 2 cut(s) 248, 567
StyD4I CCNGG 3 cut(s) 256, 316, 586
StyI CCWWGG 2 cut(s) 247, 331
TaaI ACNGT 1 cut(s) 615
TaiI ACGT 1 cut(s) 634
TasI AATT 5 cut(s) 148, 281, 311, 638, 709
TatI WGTACW 1 cut(s) 237
TauI GCSGC 1 cut(s) 59
Tru1I TTAA 4 cut(s) 138, 219, 678, 687
Tru9I TTAA 4 cut(s) 138, 219, 678, 687
TscAI CASTG 3 cut(s) 486, 618, 673
TseFI GTSAC 1 cut(s) 578
Tsp45I GTSAC 1 cut(s) 578
TspDTI ATGAA 2 cut(s) 13, 436
TspRI CASTG 3 cut(s) 486, 618, 673
Vha464I CTTAAG 1 cut(s) 686
XapI RAATTY 1 cut(s) 281
XmaJI CCTAGG 1 cut(s) 247
XmiI GTMKAC 2 cut(s) 162, 225
XspI CTAG 2 cut(s) 248, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.