FvH4_1g22090

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006879
Physical Location & Seq
Reverse (-)
14043870 .. 14048013
4144 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g22090.t1

Sequence Viewer

Length: 1266 bp
ATGTCACAACAGCAGGCCAAGTCTCATGGTTGGGCTGCCTTTGACCTCAAACAGCGTCAGAAAGGCCGGGCGCCTCAAATTGACGAGGATCCTTTCCCACCTATAGTGTCCACTGTCAAGTCTCTGCACCCAACTGTACTGAGGAATGATGAGGCTCCCAGAAAACCTTTCTCATCTGTATTTCTCCCTTCTGTAGATGTTTCATCTTTGGCAGAGAACAGAAATGGTGAAAGATCCTTATTAGATGGAAATTCTAGTAGGAAGCATACTCCCGTGGAAGATCAGAGATCTTCCATAAAGAAGATCAAGGATCATTACTCTTGGGCCGATGATAGTTTGGTGGAGGATATCATGGCTGCAGTGGATAATGATATTACTAACGCCTCAAATTTGTTAAAAGCAATGGTCTCTCCGAGCAGGTCTGAAGTGAACAAGGAAACCAGTATTTCAGGAGTAGATTCCAGTACGGATGCCTCCTTGAGCGATAAATGTTTTTCTTCAGAATCTGCTGCTGACATTGCTGAACTCAGCTCTACTATCGAGAAATGTCTCGAAGAGAATAACATAAAATGGTCAAATGACAATGATTTATACGGACAAAAGCTTTCTAATGATGCTGGAAATGTGAAACTGACTACTAGTAGTTTGGAGTCTGTACCCATTGAACCTGAGTGGGAAGAGGATGATGTTTACTTGAGGATACGAAAAGACGCATTAAGGATGATGAGGTCAGCGTCTCAGCATTCAAAAGCTGCCGCTAATGCCTTTGGAAGAGGTGACCATTATTCTGCTCAACAATACTCGATCAAGGCTAGAGAGGAATGGTTAGCTGCTGAAAGCCTTAATAACAGGGCAGCTAAGGAAATTTTAAGTATCAGGAATAGCAAAAATGATGTTTGGAAATTAGATTTACATGGTCTTCATGCATCAGAAGCAATTCGTGCCTTGCAAGAACACCTGGAGAAAATTGAAAGGAAAATACTTTCTAATCATTCAGCGTTGCCAAACAGAGCTAAGATGGAAAGCAACATTCAACATTCATCAGTTGAATCTTTTAGTTGCACGGACACAGAAATTGTGGAACAACTGCAGGTTTCAAGACAAAGACCAACATCCTTACAAGTCATAACAGGTATAGGCAATCATAGCCGAGGACAGGCTGCACTTCCAACAGCTGTGAGAAGTTTCCTTAGTGAGAATGGATATCGCTTTGAAGAGCTGAGGCCAGGTGCGATCACTGTTCGGCCCAAGTTCCGCCATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

422

Amino Acids

46.99

Weight (kDa)

6.26

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 231 - 294 7.4e-12 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 408, 1081
AccB1I GGYRCC 1 cut(s) 70
AciI CCGC 2 cut(s) 756, 1255
AclWI GGATC 4 cut(s) 83, 96, 228, 318
AcsI RAATTY 3 cut(s) 250, 388, 864
AcuI CTGAAG 2 cut(s) 444, 483
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 3 cut(s) 138, 466, 657
AfiI CCNNNNNNNGG 1 cut(s) 1156
AgsI TTSAA 7 cut(s) 665, 747, 971, 1034, 1049, 1098, 1214
AhlI ACTAGT 1 cut(s) 638
AjnI CCWGG 2 cut(s) 957, 1225
AluBI AGCT 8 cut(s) 531, 604, 752, 830, 857, 1013, 1175, 1219
AluI AGCT 8 cut(s) 531, 604, 752, 830, 857, 1013, 1175, 1219
Alw26I GTCTC 5 cut(s) 27, 126, 412, 554, 741
AlwI GGATC 4 cut(s) 83, 96, 228, 318
AlwNI CAGNNNCTG 1 cut(s) 506
AoxI GGCC 5 cut(s) 15, 64, 324, 1223, 1244
ApeKI GCWGC 7 cut(s) 35, 356, 509, 752, 830, 854, 1160
ApoI RAATTY 3 cut(s) 250, 388, 864
Asp700I GAANNNNTTC 1 cut(s) 936
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 2 cut(s) 324, 1245
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 2 cut(s) 239, 788
BamHI GGATCC 1 cut(s) 88
BanI GGYRCC 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 911
BbvCI CCTCAGC 1 cut(s) 1220
BbvI GCAGC 7 cut(s) 22, 343, 496, 739, 817, 866, 1147
BccI CCATC 2 cut(s) 239, 1012
BciT130I CCWGG 2 cut(s) 959, 1227
BciVI GTATCC 1 cut(s) 693
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 5 cut(s) 27, 126, 412, 554, 741
BcuI ACTAGT 1 cut(s) 638
BfaI CTAG 3 cut(s) 255, 639, 813
BfmI CTRYAG 4 cut(s) 102, 192, 357, 1088
BfoI RGCGCY 1 cut(s) 74
BfuAI ACCTGC 2 cut(s) 408, 1081
BfuI GTATCC 1 cut(s) 693
BglII AGATCT 1 cut(s) 287
BisI GCNGC 8 cut(s) 36, 357, 510, 753, 756, 831, 855, 1161
BlsI GCNGC 8 cut(s) 37, 358, 511, 754, 757, 832, 856, 1162
Bme1390I CCNGG 3 cut(s) 68, 959, 1227
BmgT120I GGNCC 2 cut(s) 324, 1245
BmiI GGNNCC 3 cut(s) 72, 90, 156
BmrFI CCNGG 3 cut(s) 68, 959, 1227
BmsI GCATC 3 cut(s) 460, 604, 935
BpiI GAAGAC 1 cut(s) 911
BpmI CTGGAG 1 cut(s) 980
Bpu10I CCTNAGC 2 cut(s) 858, 1220
BpuEI CTTGAG 2 cut(s) 499, 715
BpuMI CCSGG 1 cut(s) 68
BsaHI GRCGYC 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 412
BsaJI CCNNGG 2 cut(s) 273, 1150
Bsc4I CCNNNNNNNGG 1 cut(s) 1156
Bse1I ACTGG 2 cut(s) 441, 462
Bse3DI GCAATG 2 cut(s) 408, 516
BseBI CCWGG 2 cut(s) 959, 1227
BseDI CCNNGG 2 cut(s) 273, 1150
BseGI GGATG 4 cut(s) 475, 688, 726, 1112
BseLI CCNNNNNNNGG 1 cut(s) 1156
BseMI GCAATG 2 cut(s) 408, 516
BseMII CTCAG 5 cut(s) 131, 541, 660, 752, 1211
BseNI ACTGG 2 cut(s) 441, 462
BseXI GCAGC 7 cut(s) 22, 343, 496, 739, 817, 866, 1147
BsgI GTGCAG 2 cut(s) 110, 1146
BshFI GGCC 5 cut(s) 17, 66, 326, 1225, 1246
BshNI GGYRCC 1 cut(s) 70
BsiSI CCGG 1 cut(s) 67
BslI CCNNNNNNNGG 1 cut(s) 1156
BsmAI GTCTC 5 cut(s) 27, 126, 412, 554, 741
BsmBI CGTCTC 1 cut(s) 741
BsmI GAATGC 1 cut(s) 742
BsnI GGCC 5 cut(s) 17, 66, 326, 1225, 1246
Bso31I GGTCTC 1 cut(s) 412
Bsp143I GATC 8 cut(s) 88, 233, 280, 287, 303, 310, 804, 1233
BspACI CCGC 2 cut(s) 756, 1255
BspANI GGCC 5 cut(s) 17, 66, 326, 1225, 1246
BspCNI CTCAG 5 cut(s) 132, 540, 661, 751, 1212
BspLI GGNNCC 3 cut(s) 72, 90, 156
BspMAI CTGCAG 2 cut(s) 361, 1092
BspMI ACCTGC 2 cut(s) 408, 1081
BspPI GGATC 4 cut(s) 83, 96, 228, 318
BspQI GCTCTTC 1 cut(s) 1209
BspT107I GGYRCC 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 412
BsrDI GCAATG 2 cut(s) 408, 516
BsrI ACTGG 2 cut(s) 441, 462
BssECI CCNNGG 2 cut(s) 273, 1150
BssMI GATC 8 cut(s) 88, 233, 280, 287, 303, 310, 804, 1233
BssNI GRCGYC 1 cut(s) 71
Bst2UI CCWGG 2 cut(s) 959, 1227
Bst4CI ACNGT 3 cut(s) 115, 136, 1240
Bst6I CTCTTC 4 cut(s) 549, 672, 766, 1209
BstACI GRCGYC 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 941
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 8 cut(s) 140, 527, 669, 738, 858, 1014, 1190, 1220
BstDSI CCRYGG 1 cut(s) 273
BstEII GGTNACC 1 cut(s) 776
BstF5I GGATG 4 cut(s) 475, 688, 726, 1112
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 8 cut(s) 91, 236, 283, 290, 306, 313, 807, 1236
BstMAI GTCTC 5 cut(s) 27, 126, 412, 554, 741
BstMBI GATC 8 cut(s) 88, 233, 280, 287, 303, 310, 804, 1233
BstMWI GCNNNNNNNGC 5 cut(s) 518, 761, 932, 941, 1146
BstNI CCWGG 2 cut(s) 959, 1227
BstPI GGTNACC 1 cut(s) 776
BstSCI CCNGG 3 cut(s) 66, 957, 1225
BstSFI CTRYAG 4 cut(s) 102, 192, 357, 1088
BstV1I GCAGC 7 cut(s) 22, 343, 496, 739, 817, 866, 1147
BstV2I GAAGAC 1 cut(s) 911
BstX2I RGATCY 3 cut(s) 88, 233, 287
BstYI RGATCY 3 cut(s) 88, 233, 287
BsuI GTATCC 1 cut(s) 693
BsuRI GGCC 5 cut(s) 17, 66, 326, 1225, 1246
BtgI CCRYGG 1 cut(s) 273
BtsCI GGATG 4 cut(s) 475, 688, 726, 1112
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 3 cut(s) 111, 366, 1236
BveI ACCTGC 2 cut(s) 408, 1081
Cac8I GCNNGC 1 cut(s) 15
CaiI CAGNNNCTG 1 cut(s) 506
CfoI GCGC 1 cut(s) 73
Cfr13I GGNCC 2 cut(s) 324, 1245
CseI GACGC 3 cut(s) 44, 719, 723
Csp6I GTAC 3 cut(s) 137, 465, 656
CviAII CATG 4 cut(s) 26, 352, 914, 923
CviQI GTAC 3 cut(s) 137, 465, 656
DdeI CTNAG 8 cut(s) 140, 527, 669, 738, 858, 1014, 1190, 1220
DinI GGCGCC 1 cut(s) 72
DpnI GATC 8 cut(s) 90, 235, 282, 289, 305, 312, 806, 1235
DpnII GATC 8 cut(s) 88, 233, 280, 287, 303, 310, 804, 1233
Eam1104I CTCTTC 4 cut(s) 549, 672, 766, 1209
EarI CTCTTC 4 cut(s) 549, 672, 766, 1209
EciI GGCGGA 1 cut(s) 1244
Eco31I GGTCTC 1 cut(s) 412
Eco32I GATATC 2 cut(s) 349, 1205
Eco57I CTGAAG 2 cut(s) 444, 483
Eco91I GGTNACC 1 cut(s) 776
EcoO65I GGTNACC 1 cut(s) 776
EcoRII CCWGG 2 cut(s) 957, 1225
EcoRV GATATC 2 cut(s) 349, 1205
EcoT22I ATGCAT 1 cut(s) 928
EgeI GGCGCC 1 cut(s) 72
EheI GGCGCC 1 cut(s) 72
Esp3I CGTCTC 1 cut(s) 741
FaeI CATG 4 cut(s) 29, 355, 917, 926
FatI CATG 4 cut(s) 25, 351, 913, 922
Fnu4HI GCNGC 8 cut(s) 36, 357, 510, 753, 756, 831, 855, 1161
FokI GGATG 4 cut(s) 482, 695, 733, 1099
Fsp4HI GCNGC 8 cut(s) 36, 357, 510, 753, 756, 831, 855, 1161
FspBI CTAG 3 cut(s) 255, 639, 813
GlaI GCGC 1 cut(s) 72
GluI GCNGC 8 cut(s) 36, 357, 510, 753, 756, 831, 855, 1161
GsuI CTGGAG 1 cut(s) 980
HaeII RGCGCY 1 cut(s) 74
HaeIII GGCC 5 cut(s) 17, 66, 326, 1225, 1246
HapII CCGG 1 cut(s) 67
HgaI GACGC 3 cut(s) 44, 719, 723
HhaI GCGC 1 cut(s) 73
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 4 cut(s) 29, 355, 917, 926
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 4 cut(s) 458, 503, 650, 1049
HpaII CCGG 1 cut(s) 67
HphI GGTGA 2 cut(s) 239, 788
Hpy166II GTNNAC 3 cut(s) 111, 430, 691
Hpy188I TCNGA 6 cut(s) 60, 285, 414, 424, 502, 931
Hpy188III TCNNGA 5 cut(s) 450, 541, 551, 877, 1098
Hpy8I GTNNAC 3 cut(s) 111, 430, 691
HpyAV CCTTC 1 cut(s) 198
HpyCH4III ACNGT 3 cut(s) 115, 136, 1240
HpyCH4V TGCA 7 cut(s) 127, 359, 926, 949, 1062, 1090, 1163
HpyF10VI GCNNNNNNNGC 5 cut(s) 518, 761, 932, 941, 1146
HpyF3I CTNAG 8 cut(s) 140, 527, 669, 738, 858, 1014, 1190, 1220
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 4 cut(s) 29, 355, 917, 926
HspAI GCGC 1 cut(s) 71
KasI GGCGCC 1 cut(s) 70
Kzo9I GATC 8 cut(s) 88, 233, 280, 287, 303, 310, 804, 1233
LguI GCTCTTC 1 cut(s) 1209
LmnI GCTCC 1 cut(s) 160
Lsp1109I GCAGC 7 cut(s) 22, 343, 496, 739, 817, 866, 1147
LweI GCATC 3 cut(s) 460, 604, 935
MaeI CTAG 3 cut(s) 255, 639, 813
MaeIII GTNAC 2 cut(s) 3, 776
MalI GATC 8 cut(s) 90, 235, 282, 289, 305, 312, 806, 1235
MboI GATC 8 cut(s) 88, 233, 280, 287, 303, 310, 804, 1233
MboII GAAGA 9 cut(s) 282, 290, 313, 489, 566, 689, 783, 911, 1226
MflI RGATCY 3 cut(s) 88, 233, 287
MluCI AATT 8 cut(s) 78, 250, 388, 864, 902, 936, 966, 1074
Mly113I GGCGCC 1 cut(s) 71
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 1 cut(s) 1193
Mph1103I ATGCAT 1 cut(s) 928
MroXI GAANNNNTTC 1 cut(s) 936
MseI TTAA 4 cut(s) 395, 716, 843, 869
MspA1I CMGCKG 1 cut(s) 1175
MspI CCGG 1 cut(s) 67
MspR9I CCNGG 3 cut(s) 68, 959, 1227
Mva1269I GAATGC 1 cut(s) 742
MvaI CCWGG 2 cut(s) 959, 1227
MwoI GCNNNNNNNGC 5 cut(s) 518, 761, 932, 941, 1146
NarI GGCGCC 1 cut(s) 71
NciI CCSGG 1 cut(s) 68
NdeII GATC 8 cut(s) 88, 233, 280, 287, 303, 310, 804, 1233
NlaIII CATG 4 cut(s) 29, 355, 917, 926
NlaIV GGNNCC 3 cut(s) 72, 90, 156
NmeAIII GCCGAG 1 cut(s) 1175
NmuCI GTSAC 2 cut(s) 3, 776
NsiI ATGCAT 1 cut(s) 928
PciSI GCTCTTC 1 cut(s) 1209
PctI GAATGC 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 936
PfeI GAWTC 3 cut(s) 458, 503, 1049
PkrI GCNGC 8 cut(s) 37, 358, 511, 754, 757, 832, 856, 1162
PleI GAGTC 1 cut(s) 658
PluTI GGCGCC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 658
Psp6I CCWGG 2 cut(s) 957, 1225
PspEI GGTNACC 1 cut(s) 776
PspGI CCWGG 2 cut(s) 957, 1225
PspN4I GGNNCC 3 cut(s) 72, 90, 156
PspPI GGNCC 2 cut(s) 324, 1245
PstI CTGCAG 2 cut(s) 361, 1092
PstNI CAGNNNCTG 1 cut(s) 506
PsuI RGATCY 3 cut(s) 88, 233, 287
PvuII CAGCTG 1 cut(s) 1175
RsaI GTAC 3 cut(s) 138, 466, 657
RsaNI GTAC 3 cut(s) 137, 465, 656
SapI GCTCTTC 1 cut(s) 1209
SaqAI TTAA 4 cut(s) 395, 716, 843, 869
SatI GCNGC 8 cut(s) 36, 357, 510, 753, 756, 831, 855, 1161
Sau3AI GATC 8 cut(s) 88, 233, 280, 287, 303, 310, 804, 1233
Sau96I GGNCC 2 cut(s) 324, 1245
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 3 cut(s) 68, 959, 1227
SfaNI GCATC 3 cut(s) 460, 604, 935
SfcI CTRYAG 4 cut(s) 102, 192, 357, 1088
SfoI GGCGCC 1 cut(s) 72
SmlI CTYRAG 2 cut(s) 478, 694
SmoI CTYRAG 2 cut(s) 478, 694
SpeI ACTAGT 1 cut(s) 638
Sse9I AATT 8 cut(s) 78, 250, 388, 864, 902, 936, 966, 1074
SsiI CCGC 2 cut(s) 756, 1255
SspDI GGCGCC 1 cut(s) 70
SspMI CTAG 3 cut(s) 255, 639, 813
StyD4I CCNGG 3 cut(s) 66, 957, 1225
TaaI ACNGT 3 cut(s) 115, 136, 1240
TaqI TCGA 3 cut(s) 540, 552, 803
TasI AATT 8 cut(s) 78, 250, 388, 864, 902, 936, 966, 1074
TatI WGTACW 1 cut(s) 136
TauI GCSGC 1 cut(s) 758
TfiI GAWTC 3 cut(s) 458, 503, 1049
Tru1I TTAA 4 cut(s) 395, 716, 843, 869
Tru9I TTAA 4 cut(s) 395, 716, 843, 869
TscAI CASTG 3 cut(s) 118, 366, 1243
TseFI GTSAC 2 cut(s) 3, 776
TseI GCWGC 7 cut(s) 35, 356, 509, 752, 830, 854, 1160
Tsp45I GTSAC 2 cut(s) 3, 776
TspDTI ATGAA 3 cut(s) 192, 911, 1029
TspGWI ACGGA 3 cut(s) 482, 609, 1079
TspRI CASTG 3 cut(s) 118, 366, 1243
XapI RAATTY 3 cut(s) 250, 388, 864
XmnI GAANNNNTTC 1 cut(s) 936
XspI CTAG 3 cut(s) 255, 639, 813
Zsp2I ATGCAT 1 cut(s) 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.