RLG00000018245

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006879
Physical Location & Seq
Reverse (-)
27511802 .. 27516293
4492 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018245

Sequence Viewer

Length: 960 bp
ATGTCACAACAACAGGCGAAGTCTCGTGGTTGGGCTGCCTTTGACCTCAAGCAGCGTCAGAAAAAAGGCCTTGAGCCTCAAATTGACAAGGACCCTTTCCCTCCTATAGCGCCCACTATCACGTCTCTGCACCCTTATTCCAGTTCAGATGCTTCCTTGAGTGATAAAACAAGGGATAAAAGTTTTCCTTCAGAATCTGCTGCTGATATTGCTGGACTCAGCTCTACTATTGAGAAATGTCTCAAAGAGAATAACATAGAATGGTTAAATGACAATGATTTATATGGAACAAAGCTTTCTAATGGTGCTGGAAATGTGAAGCTGACTACTGGGAGTCTGGAGTCTGTACCCATTGAACCTGAGTGGGAAGAGGATGATGTTTACTTGAGGATACGAAAGGATGCATTAAGGATGATGAGGTCAGCGTCTCAGCATTCGAAAGCTGCCACTAATGCCTTTGTAAGAGGTGACCATTATTCTGCTCAACAGTACTCGATCAAGGCTAGAGAGGAATGGTTAGCTGCCGAACGCCTTAATAACAAGGCTGCTAAGGAAATTTTAAGTATCAGGAATAGCAAAAATGATGTTTGGAAATTAGATTTACATGGTCTTCATGCATCAGAAGCAATTCATGCATTGCAAGAACACCTGGAGAAAATTGAACGGAAAATACTTTCTAATGATTTAGTGTCGCCAAACAGAGTTAATATGGAAAGCAACATTAAACGTTCATCAGTTGAGTCTTTTAGTTGCATGGACACAGAAAAGTTGGATCAACTACAGGCATCATCAAGACAAAGACCAACATCCTTACAAGTCATAACAGGTATAGGCAATCATAGCCGAGGACATGCTGCACTTCCAACAGCTGTGAGAAGTTTCCTTAGTGAGAATGGATATCGTTTTGAAGAGTTGAGGCCAGGTGTGATCACTGTTCGGCCGAAGTTCCGTCATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

320

Amino Acids

35.9

Weight (kDa)

8.9

Isoelectric Point (pI)

55.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 128 - 191 5.3e-13 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 727
AclWI GGATC 1 cut(s) 780
AcoI YGGCCR 1 cut(s) 938
AcsI RAATTY 1 cut(s) 555
AcuI CTGAAG 1 cut(s) 174
AfaI GTAC 2 cut(s) 348, 491
AgsI TTSAA 3 cut(s) 356, 662, 908
AjiI CACGTC 1 cut(s) 123
AjnI CCWGG 2 cut(s) 648, 919
AluBI AGCT 6 cut(s) 222, 295, 322, 443, 521, 869
AluI AGCT 6 cut(s) 222, 295, 322, 443, 521, 869
Alw26I GTCTC 4 cut(s) 27, 129, 245, 432
AlwI GGATC 1 cut(s) 780
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 3 cut(s) 67, 917, 938
ApeKI GCWGC 7 cut(s) 35, 52, 200, 443, 521, 545, 854
ApoI RAATTY 1 cut(s) 555
Asp700I GAANNNNTTC 1 cut(s) 627
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 91
AsuHPI GGTGA 1 cut(s) 479
AsuII TTCGAA 1 cut(s) 437
AvaII GGWCC 1 cut(s) 91
BauI CACGAG 1 cut(s) 24
BbsI GAAGAC 1 cut(s) 602
BbvI GCAGC 7 cut(s) 22, 64, 187, 430, 508, 532, 841
BciT130I CCWGG 2 cut(s) 650, 921
BciVI GTATCC 1 cut(s) 384
BclI TGATCA 1 cut(s) 927
BcoDI GTCTC 4 cut(s) 27, 129, 245, 432
BfaI CTAG 1 cut(s) 504
BfmI CTRYAG 2 cut(s) 105, 779
BfoI RGCGCY 1 cut(s) 113
BfuI GTATCC 1 cut(s) 384
BisI GCNGC 7 cut(s) 36, 53, 201, 444, 522, 546, 855
BlsI GCNGC 7 cut(s) 37, 54, 202, 445, 523, 547, 856
BmcAI AGTACT 1 cut(s) 491
Bme1390I CCNGG 2 cut(s) 650, 921
Bme18I GGWCC 1 cut(s) 91
BmgBI CACGTC 1 cut(s) 123
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 93
BmrFI CCNGG 2 cut(s) 650, 921
BmrI ACTGGG 1 cut(s) 339
BmsI GCATC 4 cut(s) 139, 391, 626, 794
BmuI ACTGGG 1 cut(s) 339
BpiI GAAGAC 1 cut(s) 602
BpmI CTGGAG 2 cut(s) 359, 671
Bpu10I CCTNAGC 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 437
BpuEI CTTGAG 4 cut(s) 32, 92, 178, 406
BsaJI CCNNGG 1 cut(s) 844
Bse1I ACTGG 2 cut(s) 141, 334
Bse3DI GCAATG 1 cut(s) 635
BseBI CCWGG 2 cut(s) 650, 921
BseDI CCNNGG 1 cut(s) 844
BseGI GGATG 4 cut(s) 379, 406, 417, 806
BseMI GCAATG 1 cut(s) 635
BseMII CTCAG 3 cut(s) 232, 351, 443
BseNI ACTGG 2 cut(s) 141, 334
BseX3I CGGCCG 1 cut(s) 938
BseXI GCAGC 7 cut(s) 22, 64, 187, 430, 508, 532, 841
BsgI GTGCAG 2 cut(s) 113, 840
Bsh1285I CGRYCG 1 cut(s) 941
BshFI GGCC 3 cut(s) 69, 919, 940
BsiEI CGRYCG 1 cut(s) 941
BsmAI GTCTC 4 cut(s) 27, 129, 245, 432
BsmBI CGTCTC 2 cut(s) 129, 432
BsmI GAATGC 1 cut(s) 433
BsnI GGCC 3 cut(s) 69, 919, 940
Bsp119I TTCGAA 1 cut(s) 437
Bsp143I GATC 3 cut(s) 495, 772, 927
BspANI GGCC 3 cut(s) 69, 919, 940
BspCNI CTCAG 3 cut(s) 231, 352, 442
BspLI GGNNCC 1 cut(s) 93
BspPI GGATC 1 cut(s) 780
BspT104I TTCGAA 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 635
BsrI ACTGG 2 cut(s) 141, 334
BssECI CCNNGG 1 cut(s) 844
BssMI GATC 3 cut(s) 495, 772, 927
BssSI CACGAG 1 cut(s) 24
Bst2BI CACGAG 1 cut(s) 24
Bst2UI CCWGG 2 cut(s) 650, 921
Bst4CI ACNGT 2 cut(s) 489, 934
Bst6I CTCTTC 2 cut(s) 363, 903
BstAPI GCANNNNNTGC 1 cut(s) 632
BstBI TTCGAA 1 cut(s) 437
BstDEI CTNAG 5 cut(s) 218, 360, 429, 549, 884
BstEII GGTNACC 1 cut(s) 467
BstF5I GGATG 4 cut(s) 379, 406, 417, 806
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 3 cut(s) 498, 775, 930
BstMAI GTCTC 4 cut(s) 27, 129, 245, 432
BstMBI GATC 3 cut(s) 495, 772, 927
BstMCI CGRYCG 1 cut(s) 941
BstMWI GCNNNNNNNGC 5 cut(s) 209, 452, 623, 632, 840
BstNI CCWGG 2 cut(s) 650, 921
BstNSI RCATGY 1 cut(s) 854
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 2 cut(s) 648, 919
BstSFI CTRYAG 2 cut(s) 105, 779
BstV1I GCAGC 7 cut(s) 22, 64, 187, 430, 508, 532, 841
BstV2I GAAGAC 1 cut(s) 602
BstZI CGGCCG 1 cut(s) 938
BsuI GTATCC 1 cut(s) 384
BsuRI GGCC 3 cut(s) 69, 919, 940
BtrI CACGTC 1 cut(s) 123
BtsCI GGATG 4 cut(s) 379, 406, 417, 806
BtsIMutI CAGTG 1 cut(s) 930
CaiI CAGNNNCTG 1 cut(s) 197
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 1 cut(s) 91
CseI GACGC 2 cut(s) 44, 414
Csp6I GTAC 2 cut(s) 347, 490
CviAII CATG 5 cut(s) 605, 614, 632, 754, 851
CviQI GTAC 2 cut(s) 347, 490
DdeI CTNAG 5 cut(s) 218, 360, 429, 549, 884
DpnI GATC 3 cut(s) 497, 774, 929
DpnII GATC 3 cut(s) 495, 772, 927
EaeI YGGCCR 1 cut(s) 938
EagI CGGCCG 1 cut(s) 938
Eam1104I CTCTTC 2 cut(s) 363, 903
EarI CTCTTC 2 cut(s) 363, 903
EclXI CGGCCG 1 cut(s) 938
Eco147I AGGCCT 1 cut(s) 69
Eco32I GATATC 1 cut(s) 899
Eco47I GGWCC 1 cut(s) 91
Eco52I CGGCCG 1 cut(s) 938
Eco57I CTGAAG 1 cut(s) 174
Eco91I GGTNACC 1 cut(s) 467
EcoO109I RGGNCCY 1 cut(s) 91
EcoO65I GGTNACC 1 cut(s) 467
EcoRII CCWGG 2 cut(s) 648, 919
EcoRV GATATC 1 cut(s) 899
EcoT22I ATGCAT 3 cut(s) 406, 619, 637
Esp3I CGTCTC 2 cut(s) 129, 432
FaeI CATG 5 cut(s) 608, 617, 635, 757, 854
FalI AAGNNNNNCTT 2 cut(s) 172, 204
FatI CATG 5 cut(s) 604, 613, 631, 753, 850
FbaI TGATCA 1 cut(s) 927
Fnu4HI GCNGC 7 cut(s) 36, 53, 201, 444, 522, 546, 855
FokI GGATG 4 cut(s) 386, 413, 424, 793
Fsp4HI GCNGC 7 cut(s) 36, 53, 201, 444, 522, 546, 855
FspBI CTAG 1 cut(s) 504
GlaI GCGC 1 cut(s) 111
GluI GCNGC 7 cut(s) 36, 53, 201, 444, 522, 546, 855
GsuI CTGGAG 2 cut(s) 359, 671
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 3 cut(s) 69, 919, 940
HgaI GACGC 2 cut(s) 44, 414
HhaI GCGC 1 cut(s) 112
Hin1II CATG 5 cut(s) 608, 617, 635, 757, 854
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 5 cut(s) 194, 216, 334, 341, 740
HphI GGTGA 1 cut(s) 479
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 4 cut(s) 60, 148, 193, 622
Hpy188III TCNNGA 3 cut(s) 338, 568, 792
Hpy8I GTNNAC 1 cut(s) 382
HpyAV CCTTC 1 cut(s) 198
HpyCH4III ACNGT 2 cut(s) 489, 934
HpyCH4IV ACGT 2 cut(s) 122, 727
HpyCH4V TGCA 7 cut(s) 130, 404, 617, 635, 640, 753, 857
HpyF10VI GCNNNNNNNGC 5 cut(s) 209, 452, 623, 632, 840
HpyF3I CTNAG 5 cut(s) 218, 360, 429, 549, 884
HpySE526I ACGT 2 cut(s) 122, 727
Hsp92II CATG 5 cut(s) 608, 617, 635, 757, 854
HspAI GCGC 1 cut(s) 110
Ksp22I TGATCA 1 cut(s) 927
Kzo9I GATC 3 cut(s) 495, 772, 927
Lsp1109I GCAGC 7 cut(s) 22, 64, 187, 430, 508, 532, 841
LweI GCATC 4 cut(s) 139, 391, 626, 794
MaeI CTAG 1 cut(s) 504
MaeII ACGT 2 cut(s) 122, 727
MaeIII GTNAC 2 cut(s) 3, 467
MalI GATC 3 cut(s) 497, 774, 929
MboI GATC 3 cut(s) 495, 772, 927
MboII GAAGA 3 cut(s) 380, 602, 920
MluCI AATT 5 cut(s) 81, 555, 593, 627, 657
MlyI GAGTC 4 cut(s) 210, 343, 350, 749
MmeI TCCRAC 2 cut(s) 750, 887
Mph1103I ATGCAT 3 cut(s) 406, 619, 637
MroXI GAANNNNTTC 1 cut(s) 627
MseI TTAA 6 cut(s) 266, 407, 534, 560, 705, 723
MspA1I CMGCKG 1 cut(s) 869
MspR9I CCNGG 2 cut(s) 650, 921
Mva1269I GAATGC 1 cut(s) 433
MvaI CCWGG 2 cut(s) 650, 921
MwoI GCNNNNNNNGC 5 cut(s) 209, 452, 623, 632, 840
NdeII GATC 3 cut(s) 495, 772, 927
NlaIII CATG 5 cut(s) 608, 617, 635, 757, 854
NlaIV GGNNCC 1 cut(s) 93
NmeAIII GCCGAG 1 cut(s) 869
NmuCI GTSAC 2 cut(s) 3, 467
NsiI ATGCAT 3 cut(s) 406, 619, 637
NspI RCATGY 1 cut(s) 854
NspV TTCGAA 1 cut(s) 437
PceI AGGCCT 1 cut(s) 69
PctI GAATGC 1 cut(s) 433
PdmI GAANNNNTTC 1 cut(s) 627
PfeI GAWTC 1 cut(s) 194
PkrI GCNGC 7 cut(s) 37, 54, 202, 445, 523, 547, 856
PleI GAGTC 4 cut(s) 210, 342, 349, 748
PpsI GAGTC 4 cut(s) 210, 342, 349, 748
PpuMI RGGWCCY 1 cut(s) 91
Psp1406I AACGTT 1 cut(s) 727
Psp5II RGGWCCY 1 cut(s) 91
Psp6I CCWGG 2 cut(s) 648, 919
PspEI GGTNACC 1 cut(s) 467
PspGI CCWGG 2 cut(s) 648, 919
PspN4I GGNNCC 1 cut(s) 93
PspPI GGNCC 1 cut(s) 91
PspPPI RGGWCCY 1 cut(s) 91
PsrI GAACNNNNNNTAC 2 cut(s) 654, 686
PstNI CAGNNNCTG 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 869
RsaI GTAC 2 cut(s) 348, 491
RsaNI GTAC 2 cut(s) 347, 490
SaqAI TTAA 6 cut(s) 266, 407, 534, 560, 705, 723
SatI GCNGC 7 cut(s) 36, 53, 201, 444, 522, 546, 855
Sau3AI GATC 3 cut(s) 495, 772, 927
Sau96I GGNCC 1 cut(s) 91
ScaI AGTACT 1 cut(s) 491
SchI GAGTC 4 cut(s) 210, 343, 350, 749
ScrFI CCNGG 2 cut(s) 650, 921
SfaNI GCATC 4 cut(s) 139, 391, 626, 794
SfcI CTRYAG 2 cut(s) 105, 779
SfuI TTCGAA 1 cut(s) 437
SinI GGWCC 1 cut(s) 91
SmlI CTYRAG 4 cut(s) 47, 71, 157, 385
SmoI CTYRAG 4 cut(s) 47, 71, 157, 385
Sse9I AATT 5 cut(s) 81, 555, 593, 627, 657
SseBI AGGCCT 1 cut(s) 69
SspMI CTAG 1 cut(s) 504
StuI AGGCCT 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 648, 919
TaaI ACNGT 2 cut(s) 489, 934
TaiI ACGT 2 cut(s) 125, 730
TaqI TCGA 2 cut(s) 437, 494
TasI AATT 5 cut(s) 81, 555, 593, 627, 657
TatI WGTACW 1 cut(s) 489
TfiI GAWTC 1 cut(s) 194
Tru1I TTAA 6 cut(s) 266, 407, 534, 560, 705, 723
Tru9I TTAA 6 cut(s) 266, 407, 534, 560, 705, 723
TscAI CASTG 1 cut(s) 937
TseFI GTSAC 2 cut(s) 3, 467
TseI GCWGC 7 cut(s) 35, 52, 200, 443, 521, 545, 854
Tsp45I GTSAC 2 cut(s) 3, 467
TspDTI ATGAA 3 cut(s) 602, 620, 720
TspGWI ACGGA 2 cut(s) 679, 938
TspRI CASTG 1 cut(s) 937
VpaK11BI GGWCC 1 cut(s) 91
XapI RAATTY 1 cut(s) 555
XceI RCATGY 1 cut(s) 854
XmnI GAANNNNTTC 1 cut(s) 627
XspI CTAG 1 cut(s) 504
ZrmI AGTACT 1 cut(s) 491
Zsp2I ATGCAT 3 cut(s) 406, 619, 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.