MD15G1334300.v1.1

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006879
Physical Location & Seq
Forward (+)
37179129 .. 37183065
3937 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1334300.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGCTGCGGATGTCAAGGGCTCGAGATAAGTCTGGTGGTTGGGCTGCCTTTGACCTCAAGCAGCGCCAGAAACAAGACCTCGAGCCTCAAGTTTACAAGGATCCTTTCCTAACTATACCGACCACTCTAACTTCTCTACATCCTCGTGAAAACGTACCGAGGATTAATGACCTTCCGGGCAGGCCCTTTTCAACTCTACTCCTCCCATCTGTAGATTGTCCAACTTCGGCAGAGAATAGGGTTGGGAAAAAACCTTTATTAGATGGCGATTCCAGTGGGAAACAGTACACTTCCGTGGAAGATAATGCATCTTCCATGAGGAAGATCATGGATCTTTACCCTTGGGCTGACGATTTCTTGATTGAGGATATTATGGTTGCAATGGATAATGATATAGATAAGGCCTCAATTTTATTGAAACAATGGTTTCCTCCGGCAGCTGTGGAGAGAATAACCAAACTAGCATTTCAAAAAAACAACTCCAGCTCAAATGTCTCTTTAAGTGATAGAGAATCTGTTCCTCAATCTCCTTCTGGCATTTCTAACCTTAACTCTACGATTCAAAAATGTCTCAAAGAGAATAAGATAGAATTGTTAACTGGTGATGATTGCGGTGTGCAAAAGCTTGCTAATGATGCAGCAAATAGGAAGCTGGTTCTGGGGAGTTTGGAGTCTGTACCCATCGAACCTGAGTGGGAAGAGGATGATGTTTACTTGAAGCATCGAAAAGATGCATTAAGGATGATGAGGTCAGCATCACAGCACTCTAAGGCAGCCACTCATGCCTTTGTTAGAGGTGACCATTTTTCCGCCCAACAACACTCAAAGAAGGCTCAAGAGGAACGGTTGGTTGCTGAAAGCCTCAATAAGAAGGCAGCCAAAGAAATTTTAAGGATCAAGAATAGCAAAAATGATTTATGGAAATTGGATTTACATGGCCTTCATGCATCAGAAGCAATTCAAGCCTTGCACGAACACCTGCAGCAAATTGAAACAAAAGAGCTATCTAATTGCTCTGTGTCACCAAACAGAGTTAAGATGGAAAAGAGGATTATACGTTCTTCATCACTTGAGTCGTTTAGTTGTAAGGACAGAGAGAAATTGGATCAACAGAAGGCATCATCAACGCAAAGACCAGCATCCTTAGAGATCATCACAGGTAGAGGTAATCATAGCCGAGGACAGGCTGCACTTCCAACAGCTGTGAGAAGTTTCCTTAACGAGAATGGGTATCGTTTTGAGGAGTTGAGACCAGGTGTAATCACAGTGCGGCCCAAGTTCCGTCACAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

431

Amino Acids

48.51

Weight (kDa)

9.16

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 238 - 301 3.8e-12 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 987
Acc36I ACCTGC 1 cut(s) 987
AciI CCGC 4 cut(s) 7, 612, 810, 1270
AclWI GGATC 5 cut(s) 95, 108, 339, 902, 1113
AcsI RAATTY 1 cut(s) 885
AfaI GTAC 3 cut(s) 156, 287, 678
AfiI CCNNNNNNNGG 1 cut(s) 1183
AgsI TTSAA 7 cut(s) 192, 418, 470, 563, 718, 962, 992
AjnI CCWGG 1 cut(s) 1252
AleI CACNNNNGTG 1 cut(s) 293
AloI GAACNNNNNNTCC 2 cut(s) 1042, 1074
AluBI AGCT 6 cut(s) 440, 486, 625, 652, 1003, 1202
AluI AGCT 6 cut(s) 440, 486, 625, 652, 1003, 1202
Alw26I GTCTC 3 cut(s) 499, 575, 1243
AlwI GGATC 5 cut(s) 95, 108, 339, 902, 1113
Ama87I CYCGRG 2 cut(s) 21, 80
AoxI GGCC 4 cut(s) 182, 402, 937, 1271
ApeKI GCWGC 9 cut(s) 4, 44, 61, 437, 638, 773, 875, 982, 1187
ApoI RAATTY 1 cut(s) 885
AseI ATTAAT 1 cut(s) 165
Asp700I GAANNNNTTC 2 cut(s) 516, 957
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 2 cut(s) 183, 1272
AsuC2I CCSGG 1 cut(s) 177
AsuHPI GGTGA 3 cut(s) 614, 809, 1014
AvaI CYCGRG 2 cut(s) 21, 80
BamHI GGATCC 1 cut(s) 100
BanII GRGCYC 1 cut(s) 22
BauI CACGAG 1 cut(s) 144
BbvI GCAGC 8 cut(s) 31, 73, 449, 650, 785, 887, 994, 1174
BccI CCATC 4 cut(s) 214, 257, 689, 1033
BciT130I CCWGG 1 cut(s) 1254
BcnI CCSGG 1 cut(s) 177
BcoDI GTCTC 3 cut(s) 499, 575, 1243
BfaI CTAG 1 cut(s) 461
BfmI CTRYAG 2 cut(s) 210, 980
BfoI RGCGCY 1 cut(s) 67
BfuAI ACCTGC 1 cut(s) 987
Bme1390I CCNGG 2 cut(s) 177, 1254
BmeT110I CYCGRG 2 cut(s) 21, 80
BmgT120I GGNCC 2 cut(s) 183, 1272
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 2 cut(s) 177, 1254
BmsI GCATC 8 cut(s) 317, 625, 721, 730, 764, 956, 1127, 1148
BpmI CTGGAG 1 cut(s) 466
BpuEI CTTGAG 4 cut(s) 41, 72, 819, 1091
BpuMI CCSGG 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 1243
BsaJI CCNNGG 4 cut(s) 158, 294, 341, 1177
Bsc4I CCNNNNNNNGG 1 cut(s) 1183
Bse1I ACTGG 2 cut(s) 273, 604
Bse3DI GCAATG 1 cut(s) 387
BseBI CCWGG 1 cut(s) 1254
BseDI CCNNGG 4 cut(s) 158, 294, 341, 1177
BseGI GGATG 5 cut(s) 15, 139, 709, 747, 1139
BseLI CCNNNNNNNGG 1 cut(s) 1183
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 1 cut(s) 681
BseNI ACTGG 2 cut(s) 273, 604
BseRI GAGGAG 2 cut(s) 191, 1256
BseXI GCAGC 8 cut(s) 31, 73, 449, 650, 785, 887, 994, 1174
BsgI GTGCAG 1 cut(s) 1173
BshFI GGCC 4 cut(s) 184, 404, 939, 1273
BsiHKCI CYCGRG 2 cut(s) 21, 80
BsiSI CCGG 2 cut(s) 176, 434
BslI CCNNNNNNNGG 1 cut(s) 1183
BsmAI GTCTC 3 cut(s) 499, 575, 1243
BsnI GGCC 4 cut(s) 184, 404, 939, 1273
Bso31I GGTCTC 1 cut(s) 1243
BsoBI CYCGRG 2 cut(s) 21, 80
Bsp1286I GDGCHC 1 cut(s) 22
Bsp143I GATC 6 cut(s) 100, 324, 331, 894, 1105, 1149
BspACI CCGC 4 cut(s) 7, 612, 810, 1270
BspANI GGCC 4 cut(s) 184, 404, 939, 1273
BspCNI CTCAG 1 cut(s) 682
BspLI GGNNCC 1 cut(s) 102
BspMAI CTGCAG 1 cut(s) 984
BspMI ACCTGC 1 cut(s) 987
BspPI GGATC 5 cut(s) 95, 108, 339, 902, 1113
BspTNI GGTCTC 1 cut(s) 1243
BsrDI GCAATG 1 cut(s) 387
BsrI ACTGG 2 cut(s) 273, 604
BssECI CCNNGG 4 cut(s) 158, 294, 341, 1177
BssMI GATC 6 cut(s) 100, 324, 331, 894, 1105, 1149
BssSI CACGAG 1 cut(s) 144
BssT1I CCWWGG 1 cut(s) 341
Bst2BI CACGAG 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 1254
Bst4CI ACNGT 3 cut(s) 285, 846, 1267
Bst6I CTCTTC 1 cut(s) 693
BstC8I GCNNGC 2 cut(s) 182, 627
BstDEI CTNAG 3 cut(s) 690, 768, 1144
BstDSI CCRYGG 1 cut(s) 294
BstEII GGTNACC 1 cut(s) 797
BstF5I GGATG 5 cut(s) 15, 139, 709, 747, 1139
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 6 cut(s) 103, 327, 334, 897, 1108, 1152
BstMAI GTCTC 3 cut(s) 499, 575, 1243
BstMBI GATC 6 cut(s) 100, 324, 331, 894, 1105, 1149
BstMWI GCNNNNNNNGC 4 cut(s) 635, 782, 953, 962
BstNI CCWGG 1 cut(s) 1254
BstPI GGTNACC 1 cut(s) 797
BstSCI CCNGG 2 cut(s) 175, 1252
BstSFI CTRYAG 2 cut(s) 210, 980
BstV1I GCAGC 8 cut(s) 31, 73, 449, 650, 785, 887, 994, 1174
BstX2I RGATCY 2 cut(s) 100, 331
BstYI RGATCY 2 cut(s) 100, 331
BsuRI GGCC 4 cut(s) 184, 404, 939, 1273
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 5 cut(s) 15, 139, 709, 747, 1139
BtsIMutI CAGTG 2 cut(s) 280, 1272
BveI ACCTGC 1 cut(s) 987
Cac8I GCNNGC 2 cut(s) 182, 627
CfoI GCGC 1 cut(s) 66
Cfr13I GGNCC 2 cut(s) 183, 1272
CsiI ACCWGGT 1 cut(s) 1252
Csp6I GTAC 3 cut(s) 155, 286, 677
CviAII CATG 5 cut(s) 316, 328, 782, 935, 944
CviQI GTAC 3 cut(s) 155, 286, 677
DdeI CTNAG 3 cut(s) 690, 768, 1144
DpnI GATC 6 cut(s) 102, 326, 333, 896, 1107, 1151
DpnII GATC 6 cut(s) 100, 324, 331, 894, 1105, 1149
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
EciI GGCGGA 1 cut(s) 799
Eco130I CCWWGG 1 cut(s) 341
Eco147I AGGCCT 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 22
Eco31I GGTCTC 1 cut(s) 1243
Eco88I CYCGRG 2 cut(s) 21, 80
Eco91I GGTNACC 1 cut(s) 797
EcoO109I RGGNCCY 1 cut(s) 183
EcoO65I GGTNACC 1 cut(s) 797
EcoRII CCWGG 1 cut(s) 1252
EcoT14I CCWWGG 1 cut(s) 341
EcoT22I ATGCAT 3 cut(s) 310, 736, 949
EcoT38I GRGCYC 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 341
FaeI CATG 5 cut(s) 319, 331, 785, 938, 947
FatI CATG 5 cut(s) 315, 327, 781, 934, 943
FokI GGATG 5 cut(s) 22, 126, 716, 754, 1126
FriOI GRGCYC 1 cut(s) 22
FspBI CTAG 1 cut(s) 461
GlaI GCGC 1 cut(s) 65
GsuI CTGGAG 1 cut(s) 466
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 4 cut(s) 184, 404, 939, 1273
HapII CCGG 2 cut(s) 176, 434
HhaI GCGC 1 cut(s) 66
Hin1II CATG 5 cut(s) 319, 331, 785, 938, 947
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HincII GTYRAC 1 cut(s) 597
HindII GTYRAC 1 cut(s) 597
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 5 cut(s) 269, 512, 559, 671, 1073
HpaI GTTAAC 1 cut(s) 597
HpaII CCGG 2 cut(s) 176, 434
HphI GGTGA 3 cut(s) 614, 809, 1014
Hpy166II GTNNAC 4 cut(s) 94, 288, 597, 712
Hpy188I TCNGA 1 cut(s) 952
Hpy188III TCNNGA 5 cut(s) 23, 146, 358, 836, 898
Hpy8I GTNNAC 4 cut(s) 94, 288, 597, 712
HpyAV CCTTC 6 cut(s) 182, 540, 823, 865, 950, 1108
HpyCH4III ACNGT 3 cut(s) 285, 846, 1267
HpyCH4IV ACGT 2 cut(s) 153, 1057
HpyCH4V TGCA 9 cut(s) 308, 380, 619, 638, 734, 947, 970, 982, 1190
HpyF10VI GCNNNNNNNGC 4 cut(s) 635, 782, 953, 962
HpyF3I CTNAG 3 cut(s) 690, 768, 1144
HpySE526I ACGT 2 cut(s) 153, 1057
Hsp92II CATG 5 cut(s) 319, 331, 785, 938, 947
HspAI GCGC 1 cut(s) 64
KspAI GTTAAC 1 cut(s) 597
Kzo9I GATC 6 cut(s) 100, 324, 331, 894, 1105, 1149
Lsp1109I GCAGC 8 cut(s) 31, 73, 449, 650, 785, 887, 994, 1174
LweI GCATC 8 cut(s) 317, 625, 721, 730, 764, 956, 1127, 1148
MabI ACCWGGT 1 cut(s) 1252
MaeI CTAG 1 cut(s) 461
MaeII ACGT 2 cut(s) 153, 1057
MaeIII GTNAC 3 cut(s) 797, 1020, 1283
MalI GATC 6 cut(s) 102, 326, 333, 896, 1107, 1151
MboI GATC 6 cut(s) 100, 324, 331, 894, 1105, 1149
MboII GAAGA 5 cut(s) 303, 311, 334, 710, 1053
MflI RGATCY 2 cut(s) 100, 331
MhlI GDGCHC 1 cut(s) 22
MluCI AATT 8 cut(s) 408, 590, 885, 923, 957, 987, 1009, 1100
MlyI GAGTC 2 cut(s) 680, 1082
MmeI TCCRAC 2 cut(s) 245, 1220
Mph1103I ATGCAT 3 cut(s) 310, 736, 949
MroXI GAANNNNTTC 2 cut(s) 516, 957
MseI TTAA 8 cut(s) 165, 500, 549, 596, 737, 890, 1035, 1218
MslI CAYNNNNRTG 2 cut(s) 144, 293
MspA1I CMGCKG 2 cut(s) 440, 1202
MspI CCGG 2 cut(s) 176, 434
MspR9I CCNGG 2 cut(s) 177, 1254
MvaI CCWGG 1 cut(s) 1254
MwoI GCNNNNNNNGC 4 cut(s) 635, 782, 953, 962
NciI CCSGG 1 cut(s) 177
NdeII GATC 6 cut(s) 100, 324, 331, 894, 1105, 1149
NlaIII CATG 5 cut(s) 319, 331, 785, 938, 947
NlaIV GGNNCC 1 cut(s) 102
NmeAIII GCCGAG 1 cut(s) 1202
NmuCI GTSAC 3 cut(s) 797, 1020, 1283
NsiI ATGCAT 3 cut(s) 310, 736, 949
OliI CACNNNNGTG 1 cut(s) 293
PaeR7I CTCGAG 2 cut(s) 21, 80
PaqCI CACCTGC 1 cut(s) 987
PceI AGGCCT 1 cut(s) 404
PdmI GAANNNNTTC 2 cut(s) 516, 957
PfeI GAWTC 3 cut(s) 269, 512, 559
PleI GAGTC 2 cut(s) 679, 1081
PpsI GAGTC 2 cut(s) 679, 1081
PshBI ATTAAT 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 1252
PspEI GGTNACC 1 cut(s) 797
PspGI CCWGG 1 cut(s) 1252
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 2 cut(s) 183, 1272
PspXI VCTCGAGB 1 cut(s) 80
PstI CTGCAG 1 cut(s) 984
PsuI RGATCY 2 cut(s) 100, 331
PvuII CAGCTG 2 cut(s) 440, 1202
RsaI GTAC 3 cut(s) 156, 287, 678
RsaNI GTAC 3 cut(s) 155, 286, 677
RseI CAYNNNNRTG 2 cut(s) 144, 293
SaqAI TTAA 8 cut(s) 165, 500, 549, 596, 737, 890, 1035, 1218
Sau3AI GATC 6 cut(s) 100, 324, 331, 894, 1105, 1149
Sau96I GGNCC 2 cut(s) 183, 1272
SchI GAGTC 2 cut(s) 680, 1082
ScrFI CCNGG 2 cut(s) 177, 1254
SduI GDGCHC 1 cut(s) 22
SexAI ACCWGGT 1 cut(s) 1252
SfaNI GCATC 8 cut(s) 317, 625, 721, 730, 764, 956, 1127, 1148
SfcI CTRYAG 2 cut(s) 210, 980
Sfr274I CTCGAG 2 cut(s) 21, 80
SlaI CTCGAG 2 cut(s) 21, 80
SmiMI CAYNNNNRTG 2 cut(s) 144, 293
SmlI CTYRAG 6 cut(s) 21, 56, 80, 87, 834, 1070
SmoI CTYRAG 6 cut(s) 21, 56, 80, 87, 834, 1070
Sse9I AATT 8 cut(s) 408, 590, 885, 923, 957, 987, 1009, 1100
SseBI AGGCCT 1 cut(s) 404
SsiI CCGC 4 cut(s) 7, 612, 810, 1270
SspMI CTAG 1 cut(s) 461
StuI AGGCCT 1 cut(s) 404
StyD4I CCNGG 2 cut(s) 175, 1252
StyI CCWWGG 1 cut(s) 341
TaaI ACNGT 3 cut(s) 285, 846, 1267
TaiI ACGT 2 cut(s) 156, 1060
TaqI TCGA 4 cut(s) 22, 81, 684, 724
TasI AATT 8 cut(s) 408, 590, 885, 923, 957, 987, 1009, 1100
TatI WGTACW 1 cut(s) 285
TauI GCSGC 1 cut(s) 1273
TfiI GAWTC 3 cut(s) 269, 512, 559
Tru1I TTAA 8 cut(s) 165, 500, 549, 596, 737, 890, 1035, 1218
Tru9I TTAA 8 cut(s) 165, 500, 549, 596, 737, 890, 1035, 1218
TscAI CASTG 2 cut(s) 280, 1272
TseFI GTSAC 3 cut(s) 797, 1020, 1283
TseI GCWGC 9 cut(s) 4, 44, 61, 437, 638, 773, 875, 982, 1187
Tsp45I GTSAC 3 cut(s) 797, 1020, 1283
TspDTI ATGAA 2 cut(s) 932, 1053
TspGWI ACGGA 2 cut(s) 283, 1271
TspRI CASTG 2 cut(s) 280, 1272
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 1 cut(s) 885
XhoI CTCGAG 2 cut(s) 21, 80
XmnI GAANNNNTTC 2 cut(s) 516, 957
XspI CTAG 1 cut(s) 461
Zsp2I ATGCAT 3 cut(s) 310, 736, 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.