Rmu_sc0006942.1_g000002

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006942.1
OG0006879
Physical Location & Seq
Forward (+)
8061 .. 8831
771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006942.1_g000002.1.cds

Sequence Viewer

Length: 771 bp
atgtcacaacaacaggcgaagtctcgtggttgggctgcctttgacctcaagcagcgtcagaaaaaaggccttgagcctcaaattgagaaggaccctttccctcctatagcgcccactatcacgtctctgcacccttgtgaaaatgcactgaggaataatgaggttccgagcaaacctttctcatctgtattcctctcttctgcacatgttgtatctatggcagagaatagggatggtaaaagatccttattagatggaaattctagtaggaagcatacttccatggaagatcagagatcttccataaagaagatcaaggatctttactcttgggctgatgatagtttggtggaggatattatggctgcagtggataatgatattactaaggcctcaaatttattaaaaacaatggtctccccgggaagatctgaagagaataaggaaaccaacatttcaaaaatagattccagttcagatgcttccttgagcgataaaacaagggataaaagttttccttcagaatctgcagctgatattgctggactcagctctactattgagaaatgtctcaaagagaataacatagaatggttaaatgacaatgatttatatggaccaaagctttctaatggtgctggaaatgtgaaactgactactgggagtctggagtctgtacccattgaacctgagtgggaagaggatgatgtttacttgaggatacgaaaggatgcattaaggatgatgaggtatagtacttggctcttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

256

Amino Acids

28.62

Weight (kDa)

5.43

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 237, 327
AcsI RAATTY 2 cut(s) 259, 397
AcuI CTGAAG 2 cut(s) 453, 504
AfaI GTAC 2 cut(s) 678, 757
AflIII ACRYGT 1 cut(s) 205
AgsI TTSAA 2 cut(s) 459, 686
AjiI CACGTC 1 cut(s) 123
AleI CACNNNNGTG 1 cut(s) 135
AluBI AGCT 3 cut(s) 533, 552, 625
AluI AGCT 3 cut(s) 533, 552, 625
Alw26I GTCTC 4 cut(s) 27, 129, 421, 575
AlwI GGATC 2 cut(s) 237, 327
AlwNI CAGNNNCTG 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 421
AoxI GGCC 2 cut(s) 67, 390
ApeKI GCWGC 4 cut(s) 35, 52, 365, 530
ApoI RAATTY 2 cut(s) 259, 397
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 2 cut(s) 91, 617
AsuC2I CCSGG 2 cut(s) 422, 423
AvaI CYCGRG 1 cut(s) 421
AvaII GGWCC 2 cut(s) 91, 617
BauI CACGAG 1 cut(s) 24
BbvI GCAGC 4 cut(s) 22, 64, 352, 542
BccI CCATC 2 cut(s) 227, 248
BciVI GTATCC 1 cut(s) 714
BcnI CCSGG 2 cut(s) 422, 423
BcoDI GTCTC 4 cut(s) 27, 129, 421, 575
BfaI CTAG 1 cut(s) 264
BfmI CTRYAG 3 cut(s) 105, 366, 528
BfoI RGCGCY 1 cut(s) 113
BfuI GTATCC 1 cut(s) 714
BglII AGATCT 2 cut(s) 296, 428
BisI GCNGC 4 cut(s) 36, 53, 366, 531
BlsI GCNGC 4 cut(s) 37, 54, 367, 532
BmcAI AGTACT 1 cut(s) 757
Bme1390I CCNGG 2 cut(s) 422, 423
Bme18I GGWCC 2 cut(s) 91, 617
BmeT110I CYCGRG 1 cut(s) 421
BmgBI CACGTC 1 cut(s) 123
BmgT120I GGNCC 2 cut(s) 91, 617
BmiI GGNNCC 2 cut(s) 93, 165
BmrFI CCNGG 2 cut(s) 422, 423
BmrI ACTGGG 1 cut(s) 669
BmsI GCATC 2 cut(s) 469, 721
BmuI ACTGGG 1 cut(s) 669
BpmI CTGGAG 1 cut(s) 689
BpuEI CTTGAG 4 cut(s) 32, 92, 508, 736
BpuMI CCSGG 2 cut(s) 422, 423
BsaI GGTCTC 1 cut(s) 421
BsaJI CCNNGG 3 cut(s) 282, 420, 421
Bse1I ACTGG 2 cut(s) 471, 664
BseDI CCNNGG 3 cut(s) 282, 420, 421
BseGI GGATG 4 cut(s) 238, 709, 736, 747
BseMII CTCAG 3 cut(s) 140, 562, 681
BseNI ACTGG 2 cut(s) 471, 664
BseXI GCAGC 4 cut(s) 22, 64, 352, 542
BsgI GTGCAG 2 cut(s) 113, 186
BshFI GGCC 2 cut(s) 69, 392
BsiHKCI CYCGRG 1 cut(s) 421
BsiSI CCGG 1 cut(s) 422
BsmAI GTCTC 4 cut(s) 27, 129, 421, 575
BsmBI CGTCTC 1 cut(s) 129
BsnI GGCC 2 cut(s) 69, 392
Bso31I GGTCTC 1 cut(s) 421
BsoBI CYCGRG 1 cut(s) 421
Bsp143I GATC 6 cut(s) 242, 289, 296, 312, 319, 428
Bsp19I CCATGG 1 cut(s) 282
BspANI GGCC 2 cut(s) 69, 392
BspCNI CTCAG 3 cut(s) 141, 561, 682
BspLI GGNNCC 2 cut(s) 93, 165
BspMAI CTGCAG 2 cut(s) 370, 532
BspPI GGATC 2 cut(s) 237, 327
BspTNI GGTCTC 1 cut(s) 421
BsrI ACTGG 2 cut(s) 471, 664
BssECI CCNNGG 3 cut(s) 282, 420, 421
BssMI GATC 6 cut(s) 242, 289, 296, 312, 319, 428
BssSI CACGAG 1 cut(s) 24
BssT1I CCWWGG 1 cut(s) 282
Bst2BI CACGAG 1 cut(s) 24
Bst6I CTCTTC 3 cut(s) 202, 429, 693
BstDEI CTNAG 4 cut(s) 149, 387, 548, 690
BstDSI CCRYGG 1 cut(s) 282
BstF5I GGATG 4 cut(s) 238, 709, 736, 747
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 6 cut(s) 245, 292, 299, 315, 322, 431
BstMAI GTCTC 4 cut(s) 27, 129, 421, 575
BstMBI GATC 6 cut(s) 242, 289, 296, 312, 319, 428
BstMWI GCNNNNNNNGC 1 cut(s) 539
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 420, 421
BstSFI CTRYAG 3 cut(s) 105, 366, 528
BstV1I GCAGC 4 cut(s) 22, 64, 352, 542
BstX2I RGATCY 4 cut(s) 242, 296, 319, 428
BstYI RGATCY 4 cut(s) 242, 296, 319, 428
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 2 cut(s) 69, 392
BtgI CCRYGG 1 cut(s) 282
BtrI CACGTC 1 cut(s) 123
BtsCI GGATG 4 cut(s) 238, 709, 736, 747
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 146, 375
CaiI CAGNNNCTG 1 cut(s) 527
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 91, 617
Cfr9I CCCGGG 1 cut(s) 421
CseI GACGC 1 cut(s) 44
Csp6I GTAC 2 cut(s) 677, 756
CviAII CATG 2 cut(s) 206, 283
CviQI GTAC 2 cut(s) 677, 756
DdeI CTNAG 4 cut(s) 149, 387, 548, 690
DpnI GATC 6 cut(s) 244, 291, 298, 314, 321, 430
DpnII GATC 6 cut(s) 242, 289, 296, 312, 319, 428
Eam1104I CTCTTC 3 cut(s) 202, 429, 693
EarI CTCTTC 3 cut(s) 202, 429, 693
Eco130I CCWWGG 1 cut(s) 282
Eco147I AGGCCT 2 cut(s) 69, 392
Eco31I GGTCTC 1 cut(s) 421
Eco47I GGWCC 2 cut(s) 91, 617
Eco57I CTGAAG 2 cut(s) 453, 504
Eco88I CYCGRG 1 cut(s) 421
EcoO109I RGGNCCY 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 282
EcoT22I ATGCAT 1 cut(s) 736
ErhI CCWWGG 1 cut(s) 282
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 2 cut(s) 209, 286
FalI AAGNNNNNCTT 2 cut(s) 502, 534
FatI CATG 2 cut(s) 205, 282
Fnu4HI GCNGC 4 cut(s) 36, 53, 366, 531
FokI GGATG 4 cut(s) 245, 716, 743, 754
Fsp4HI GCNGC 4 cut(s) 36, 53, 366, 531
FspBI CTAG 1 cut(s) 264
GlaI GCGC 1 cut(s) 111
GluI GCNGC 4 cut(s) 36, 53, 366, 531
GsuI CTGGAG 1 cut(s) 689
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 2 cut(s) 69, 392
HapII CCGG 1 cut(s) 422
HgaI GACGC 1 cut(s) 44
HhaI GCGC 1 cut(s) 112
Hin1II CATG 2 cut(s) 209, 286
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 5 cut(s) 467, 524, 546, 664, 671
HpaII CCGG 1 cut(s) 422
Hpy166II GTNNAC 1 cut(s) 712
Hpy188I TCNGA 6 cut(s) 60, 168, 294, 433, 478, 523
Hpy188III TCNNGA 1 cut(s) 668
Hpy8I GTNNAC 1 cut(s) 712
HpyAV CCTTC 2 cut(s) 82, 528
HpyCH4IV ACGT 1 cut(s) 122
HpyCH4V TGCA 6 cut(s) 130, 146, 203, 368, 530, 734
HpyF10VI GCNNNNNNNGC 1 cut(s) 539
HpyF3I CTNAG 4 cut(s) 149, 387, 548, 690
HpySE526I ACGT 1 cut(s) 122
Hsp92II CATG 2 cut(s) 209, 286
HspAI GCGC 1 cut(s) 110
Kzo9I GATC 6 cut(s) 242, 289, 296, 312, 319, 428
LpnPI CCDG 7 cut(s) 435, 484, 528, 624, 645, 653, 702
Lsp1109I GCAGC 4 cut(s) 22, 64, 352, 542
LweI GCATC 2 cut(s) 469, 721
MaeI CTAG 1 cut(s) 264
MaeII ACGT 1 cut(s) 122
MaeIII GTNAC 1 cut(s) 3
MalI GATC 6 cut(s) 244, 291, 298, 314, 321, 430
MboI GATC 6 cut(s) 242, 289, 296, 312, 319, 428
MboII GAAGA 8 cut(s) 189, 291, 299, 322, 438, 446, 710, 757
MflI RGATCY 4 cut(s) 242, 296, 319, 428
MluCI AATT 3 cut(s) 81, 259, 397
MlyI GAGTC 3 cut(s) 540, 673, 680
Mph1103I ATGCAT 1 cut(s) 736
MseI TTAA 3 cut(s) 404, 596, 737
MslI CAYNNNNRTG 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 2 cut(s) 422, 423
MwoI GCNNNNNNNGC 1 cut(s) 539
NciI CCSGG 2 cut(s) 422, 423
NcoI CCATGG 1 cut(s) 282
NdeII GATC 6 cut(s) 242, 289, 296, 312, 319, 428
NlaIII CATG 2 cut(s) 209, 286
NlaIV GGNNCC 2 cut(s) 93, 165
NmuCI GTSAC 1 cut(s) 3
NsiI ATGCAT 1 cut(s) 736
NspI RCATGY 1 cut(s) 209
OliI CACNNNNGTG 1 cut(s) 135
PceI AGGCCT 2 cut(s) 69, 392
PciI ACATGT 1 cut(s) 205
PfeI GAWTC 2 cut(s) 467, 524
PkrI GCNGC 4 cut(s) 37, 54, 367, 532
PleI GAGTC 3 cut(s) 540, 672, 679
PpsI GAGTC 3 cut(s) 540, 672, 679
PpuMI RGGWCCY 1 cut(s) 91
PscI ACATGT 1 cut(s) 205
Psp5II RGGWCCY 1 cut(s) 91
PspN4I GGNNCC 2 cut(s) 93, 165
PspPI GGNCC 2 cut(s) 91, 617
PspPPI RGGWCCY 1 cut(s) 91
PstI CTGCAG 2 cut(s) 370, 532
PstNI CAGNNNCTG 1 cut(s) 527
PsuI RGATCY 4 cut(s) 242, 296, 319, 428
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 2 cut(s) 678, 757
RsaNI GTAC 2 cut(s) 677, 756
RseI CAYNNNNRTG 1 cut(s) 135
SaqAI TTAA 3 cut(s) 404, 596, 737
SatI GCNGC 4 cut(s) 36, 53, 366, 531
Sau3AI GATC 6 cut(s) 242, 289, 296, 312, 319, 428
Sau96I GGNCC 2 cut(s) 91, 617
ScaI AGTACT 1 cut(s) 757
SchI GAGTC 3 cut(s) 540, 673, 680
ScrFI CCNGG 2 cut(s) 422, 423
SetI ASST 9 cut(s) 48, 125, 165, 178, 535, 554, 627, 691, 752
SfaNI GCATC 2 cut(s) 469, 721
SfcI CTRYAG 3 cut(s) 105, 366, 528
SinI GGWCC 2 cut(s) 91, 617
SmaI CCCGGG 1 cut(s) 423
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 4 cut(s) 47, 71, 487, 715
SmoI CTYRAG 4 cut(s) 47, 71, 487, 715
Sse9I AATT 3 cut(s) 81, 259, 397
SseBI AGGCCT 2 cut(s) 69, 392
SspMI CTAG 1 cut(s) 264
StuI AGGCCT 2 cut(s) 69, 392
StyD4I CCNGG 2 cut(s) 420, 421
StyI CCWWGG 1 cut(s) 282
TaiI ACGT 1 cut(s) 125
TasI AATT 3 cut(s) 81, 259, 397
TatI WGTACW 1 cut(s) 755
TfiI GAWTC 2 cut(s) 467, 524
Tru1I TTAA 3 cut(s) 404, 596, 737
Tru9I TTAA 3 cut(s) 404, 596, 737
TscAI CASTG 2 cut(s) 153, 375
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 4 cut(s) 35, 52, 365, 530
Tsp45I GTSAC 1 cut(s) 3
TspMI CCCGGG 1 cut(s) 421
TspRI CASTG 2 cut(s) 153, 375
VpaK11BI GGWCC 2 cut(s) 91, 617
XapI RAATTY 2 cut(s) 259, 397
XceI RCATGY 1 cut(s) 209
XmaI CCCGGG 1 cut(s) 421
XspI CTAG 1 cut(s) 264
ZrmI AGTACT 1 cut(s) 757
Zsp2I ATGCAT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.