Rorug02G0204400

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006879
Physical Location & Seq
Forward (+)
19153800 .. 19155058
1259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0204400.1

Sequence Viewer

Length: 369 bp
ATGGGTTTTCGCTACACCACCAGTTCTATCATCTTTCTGATGATTTTGAGCTCTACCCAGATAAGATTCATGGCTGAAAGCAGAGTACTTCTCAAGAAGGATGACACTTCCCAGACAGTGAATGAAGACAATAAGGCAAGACTGAGAGCACAAATTGGTTCAAGGCCTCCAAGGTGTGAAGGAAGGTGCAGCTCATGTGGCCCTTGTGAGGCAATTCAAGTTCCTAACAATTTTCAAGCCAAACCAGGTAAAAGGAACTCTTCATCAGTTGTAACCAACATTGCATATGCCAGAGGAGGCGAGTATTCAAGCAACTACAAGCCCATGAGTTGGAAATGCAAATGTGGCACCATCATCTTCAACCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

122

Amino Acids

13.6

Weight (kDa)

9.69

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 52 - 122 7.4e-18 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 347
AccB7I CCANNNNNTGG 1 cut(s) 330
AfaI GTAC 1 cut(s) 87
AfiI CCNNNNNNNGG 2 cut(s) 208, 330
AgsI TTSAA 5 cut(s) 162, 218, 236, 309, 361
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 2 cut(s) 51, 192
AluI AGCT 2 cut(s) 51, 192
Alw21I GWGCWC 2 cut(s) 53, 151
AoxI GGCC 2 cut(s) 164, 199
ApeKI GCWGC 1 cut(s) 189
AspS9I GGNCC 1 cut(s) 200
BanI GGYRCC 1 cut(s) 347
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 2 cut(s) 53, 151
BbvI GCAGC 1 cut(s) 201
BccI CCATC 1 cut(s) 359
BciT130I CCWGG 1 cut(s) 246
BisI GCNGC 1 cut(s) 190
BlsI GCNGC 1 cut(s) 191
BmcAI AGTACT 1 cut(s) 87
Bme1390I CCNGG 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 1 cut(s) 349
BmrFI CCNGG 1 cut(s) 246
BpiI GAAGAC 1 cut(s) 132
BplI GAGNNNNNCTC 2 cut(s) 75, 107
BpuEI CTTGAG 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 170
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 330
Bse1I ACTGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 279
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 170
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 2 cut(s) 208, 330
BseMI GCAATG 1 cut(s) 279
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 1 cut(s) 309
BseXI GCAGC 1 cut(s) 201
BsgI GTGCAG 1 cut(s) 208
BshFI GGCC 2 cut(s) 166, 201
BshNI GGYRCC 1 cut(s) 347
BsiHKAI GWGCWC 2 cut(s) 53, 151
BslI CCNNNNNNNGG 2 cut(s) 208, 330
BsnI GGCC 2 cut(s) 166, 201
Bsp1286I GDGCHC 2 cut(s) 53, 151
BspANI GGCC 2 cut(s) 166, 201
BspCNI CTCAG 1 cut(s) 135
BspLI GGNNCC 1 cut(s) 349
BspT107I GGYRCC 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 279
BsrI ACTGG 1 cut(s) 21
BssECI CCNNGG 1 cut(s) 170
BssT1I CCWWGG 1 cut(s) 170
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 1 cut(s) 265
BstDEI CTNAG 1 cut(s) 143
BstF5I GGATG 1 cut(s) 106
BstMWI GCNNNNNNNGC 2 cut(s) 198, 345
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 244
BstV1I GCAGC 1 cut(s) 201
BstV2I GAAGAC 1 cut(s) 132
BsuRI GGCC 2 cut(s) 166, 201
BtsCI GGATG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 123
Cfr13I GGNCC 1 cut(s) 200
CsiI ACCWGGT 1 cut(s) 244
Csp6I GTAC 1 cut(s) 86
CviAII CATG 3 cut(s) 70, 195, 325
CviJI RGCY 7 cut(s) 51, 74, 166, 192, 201, 239, 322
CviKI_1 RGCY 7 cut(s) 51, 74, 166, 192, 201, 239, 322
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 1 cut(s) 143
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 170
Eco147I AGGCCT 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 53
Eco53kI GAGCTC 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 51
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 170
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 170
FaeI CATG 3 cut(s) 73, 198, 328
FaiI YATR 5 cut(s) 71, 196, 286, 288, 326
FalI AAGNNNNNCTT 2 cut(s) 244, 276
FatI CATG 3 cut(s) 69, 194, 324
FauNDI CATATG 1 cut(s) 286
Fnu4HI GCNGC 1 cut(s) 190
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 1 cut(s) 190
GluI GCNGC 1 cut(s) 190
HaeIII GGCC 2 cut(s) 166, 201
Hin1II CATG 3 cut(s) 73, 198, 328
HinfI GANTC 1 cut(s) 66
Hpy188I TCNGA 1 cut(s) 39
Hpy188III TCNNGA 1 cut(s) 94
HpyAV CCTTC 3 cut(s) 91, 173, 177
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4V TGCA 3 cut(s) 189, 284, 339
HpyF10VI GCNNNNNNNGC 2 cut(s) 198, 345
HpyF3I CTNAG 1 cut(s) 143
Hsp92II CATG 3 cut(s) 73, 198, 328
LpnPI CCDG 6 cut(s) 34, 71, 125, 231, 258, 304
Lsp1109I GCAGC 1 cut(s) 201
MabI ACCWGGT 1 cut(s) 244
MaeIII GTNAC 1 cut(s) 271
MboII GAAGA 3 cut(s) 137, 252, 349
MhlI GDGCHC 2 cut(s) 53, 151
MluCI AATT 3 cut(s) 153, 213, 229
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 4 cut(s) 177, 202, 287, 290
MspR9I CCNGG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 246
MwoI GCNNNNNNNGC 2 cut(s) 198, 345
NdeI CATATG 1 cut(s) 286
NlaIII CATG 3 cut(s) 73, 198, 328
NlaIV GGNNCC 1 cut(s) 349
PceI AGGCCT 1 cut(s) 166
PfeI GAWTC 1 cut(s) 66
PflMI CCANNNNNTGG 1 cut(s) 330
PkrI GCNGC 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 53
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 349
PspPI GGNCC 1 cut(s) 200
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
SacI GAGCTC 1 cut(s) 53
SatI GCNGC 1 cut(s) 190
Sau96I GGNCC 1 cut(s) 200
ScaI AGTACT 1 cut(s) 87
ScrFI CCNGG 1 cut(s) 246
SduI GDGCHC 2 cut(s) 53, 151
SetI ASST 5 cut(s) 53, 176, 188, 194, 250
SexAI ACCWGGT 1 cut(s) 244
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 3 cut(s) 153, 213, 229
SseBI AGGCCT 1 cut(s) 166
SstI GAGCTC 1 cut(s) 53
StuI AGGCCT 1 cut(s) 166
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 1 cut(s) 118
TasI AATT 3 cut(s) 153, 213, 229
TatI WGTACW 1 cut(s) 85
TfiI GAWTC 1 cut(s) 66
TscAI CASTG 1 cut(s) 123
TseI GCWGC 1 cut(s) 189
TspDTI ATGAA 3 cut(s) 58, 138, 252
TspRI CASTG 1 cut(s) 123
Van91I CCANNNNNTGG 1 cut(s) 330
ZrmI AGTACT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.