Rw2G020400

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006879
Physical Location & Seq
Reverse (-)
27846512 .. 27852457
5946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020400.1

Sequence Viewer

Length: 1230 bp
ATGTCACAACAACAGGCGAAGTCTCGTGGTTGGGCTGCCTTTGACCTCAAGCAGCGTCAGAAAAAAGGCCTTGAGCCTCAAATTGAGAAGGACCCTTTCCCTCCTATAGCGCCCACTATCACGTCTCTGCACCCCTGTGAAAATGCACTGAGGAATAATGAGGTTCCGAGCAAACCTTTCTCATCTGTATTCCTCTCTTCTGCACATGTTGTATCTTTGGCAGAGAATAGGGATGGTAAAAGATCCTTATTAGATGGAAATTCTAGTAGGAAGCATACTTCCATGGAAGATCAGAGATCTTCCATAAAGAAGATCAAGGATCTTTACTCTTGGGCTGATGATAGTTTGGTGGAGGATATTATGGCTGCAGTGGATAATGATATTACTAAGGCCTCAAATTTATTAAAAACAATGGTCTCCCCGGGAAGATCTGAAGAGAATAAGGAAACCAACATTTCAAAAATAGATTCCAGTTCAGATGCTTCCTTGAGCGATAAAACAAGGGATAAAAGTTTTCCTTCAGAATCTGCTGCTGATATTGCTGGACTCAGCTCTACTATTGAGAAATGTCTCAAAGAGAATAACATAGAATGGTTAAATGACAATGATTTATATGGACCAAAGCTTTCTAATGGTGCTGGAAATGTGAAACTGACTACTGGGAGTCTGGAGTCTGTACCCATTGAACCTGAGTGGGAAGAGGATGATGTTTACTTGAGGATACGAAAGGATGCATTAAGGATGATGAGGTCAGCGTCTCAGCATTCGAAAGCTGCCACTAATGCCTTTGTAAGAGGTGACCATTATTCTGCTCAACAGTACTCGATCAAGGCTAGAGAGGAATGGTTAGCTGCCGAACGCCTTAATAACAAGGCTGCTAAGGAAATTTTAAGTATCAGGAATAGCAAAAATGATGTTTGGAAATTAGATTTACATGGTCTTCATGCATCAGAAGCAATTCATGCATTGCAAGAACACCTGGAGAAAATTGAACGGAAAATACTTTCTAATGATTTAGTGTCGCCAAACAGAGTTAATATGGAAAGCAACATTAAACGTTCATCAGTTGAGTCTTTTAGTTGCATGGACACAGAAAAGTTGGATCAACTACAGGCATCATCAAGACAAAGACCAACATCCTTACAAGTCATAACAGGTATAGGCAATCATAGCCGAGGACATGCTGCACTTCCAACAGCTGTGAGAAGTTTCCTTAGTGAGAATGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

409

Amino Acids

45.51

Weight (kDa)

6.62

Isoelectric Point (pI)

55.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 238 - 301 7.9e-13 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 1057
AclWI GGATC 3 cut(s) 237, 327, 1110
AcsI RAATTY 3 cut(s) 259, 397, 885
AcuI CTGAAG 2 cut(s) 453, 504
AfaI GTAC 2 cut(s) 678, 821
AflIII ACRYGT 1 cut(s) 205
AgsI TTSAA 3 cut(s) 459, 686, 992
AjiI CACGTC 1 cut(s) 123
AjnI CCWGG 1 cut(s) 978
AleI CACNNNNGTG 1 cut(s) 135
AluBI AGCT 5 cut(s) 552, 625, 773, 851, 1199
AluI AGCT 5 cut(s) 552, 625, 773, 851, 1199
Alw26I GTCTC 5 cut(s) 27, 129, 421, 575, 762
AlwI GGATC 3 cut(s) 237, 327, 1110
AlwNI CAGNNNCTG 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 421
AoxI GGCC 2 cut(s) 67, 390
ApeKI GCWGC 8 cut(s) 35, 52, 365, 530, 773, 851, 875, 1184
ApoI RAATTY 3 cut(s) 259, 397, 885
Asp700I GAANNNNTTC 1 cut(s) 957
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 2 cut(s) 91, 617
AsuC2I CCSGG 2 cut(s) 422, 423
AsuHPI GGTGA 1 cut(s) 809
AsuII TTCGAA 1 cut(s) 767
AvaI CYCGRG 1 cut(s) 421
AvaII GGWCC 2 cut(s) 91, 617
BauI CACGAG 1 cut(s) 24
BbsI GAAGAC 1 cut(s) 932
BbvI GCAGC 8 cut(s) 22, 64, 352, 517, 760, 838, 862, 1171
BccI CCATC 2 cut(s) 227, 248
BciT130I CCWGG 1 cut(s) 980
BciVI GTATCC 1 cut(s) 714
BcnI CCSGG 2 cut(s) 422, 423
BcoDI GTCTC 5 cut(s) 27, 129, 421, 575, 762
BfaI CTAG 2 cut(s) 264, 834
BfmI CTRYAG 3 cut(s) 105, 366, 1109
BfoI RGCGCY 1 cut(s) 113
BfuI GTATCC 1 cut(s) 714
BglII AGATCT 2 cut(s) 296, 428
BisI GCNGC 8 cut(s) 36, 53, 366, 531, 774, 852, 876, 1185
BlsI GCNGC 8 cut(s) 37, 54, 367, 532, 775, 853, 877, 1186
BmcAI AGTACT 1 cut(s) 821
Bme1390I CCNGG 3 cut(s) 422, 423, 980
Bme18I GGWCC 2 cut(s) 91, 617
BmeT110I CYCGRG 1 cut(s) 421
BmgBI CACGTC 1 cut(s) 123
BmgT120I GGNCC 2 cut(s) 91, 617
BmiI GGNNCC 2 cut(s) 93, 165
BmrFI CCNGG 3 cut(s) 422, 423, 980
BmrI ACTGGG 1 cut(s) 669
BmsI GCATC 4 cut(s) 469, 721, 956, 1124
BmuI ACTGGG 1 cut(s) 669
BpiI GAAGAC 1 cut(s) 932
BpmI CTGGAG 2 cut(s) 689, 1001
Bpu10I CCTNAGC 1 cut(s) 879
Bpu14I TTCGAA 1 cut(s) 767
BpuEI CTTGAG 4 cut(s) 32, 92, 508, 736
BpuMI CCSGG 2 cut(s) 422, 423
BsaI GGTCTC 1 cut(s) 421
BsaJI CCNNGG 4 cut(s) 282, 420, 421, 1174
Bse1I ACTGG 2 cut(s) 471, 664
Bse3DI GCAATG 1 cut(s) 965
BseBI CCWGG 1 cut(s) 980
BseDI CCNNGG 4 cut(s) 282, 420, 421, 1174
BseGI GGATG 5 cut(s) 238, 709, 736, 747, 1136
BseMI GCAATG 1 cut(s) 965
BseMII CTCAG 4 cut(s) 140, 562, 681, 773
BseNI ACTGG 2 cut(s) 471, 664
BseXI GCAGC 8 cut(s) 22, 64, 352, 517, 760, 838, 862, 1171
BsgI GTGCAG 3 cut(s) 113, 186, 1170
BshFI GGCC 2 cut(s) 69, 392
BsiHKCI CYCGRG 1 cut(s) 421
BsiSI CCGG 1 cut(s) 422
BsmAI GTCTC 5 cut(s) 27, 129, 421, 575, 762
BsmBI CGTCTC 2 cut(s) 129, 762
BsmI GAATGC 1 cut(s) 763
BsnI GGCC 2 cut(s) 69, 392
Bso31I GGTCTC 1 cut(s) 421
BsoBI CYCGRG 1 cut(s) 421
Bsp119I TTCGAA 1 cut(s) 767
Bsp143I GATC 8 cut(s) 242, 289, 296, 312, 319, 428, 825, 1102
Bsp19I CCATGG 1 cut(s) 282
BspANI GGCC 2 cut(s) 69, 392
BspCNI CTCAG 4 cut(s) 141, 561, 682, 772
BspLI GGNNCC 2 cut(s) 93, 165
BspMAI CTGCAG 1 cut(s) 370
BspPI GGATC 3 cut(s) 237, 327, 1110
BspT104I TTCGAA 1 cut(s) 767
BspTNI GGTCTC 1 cut(s) 421
BsrDI GCAATG 1 cut(s) 965
BsrI ACTGG 2 cut(s) 471, 664
BssECI CCNNGG 4 cut(s) 282, 420, 421, 1174
BssMI GATC 8 cut(s) 242, 289, 296, 312, 319, 428, 825, 1102
BssSI CACGAG 1 cut(s) 24
BssT1I CCWWGG 1 cut(s) 282
Bst2BI CACGAG 1 cut(s) 24
Bst2UI CCWGG 1 cut(s) 980
Bst4CI ACNGT 1 cut(s) 819
Bst6I CTCTTC 3 cut(s) 202, 429, 693
BstAPI GCANNNNNTGC 1 cut(s) 962
BstBI TTCGAA 1 cut(s) 767
BstDEI CTNAG 7 cut(s) 149, 387, 548, 690, 759, 879, 1214
BstDSI CCRYGG 1 cut(s) 282
BstEII GGTNACC 1 cut(s) 797
BstF5I GGATG 5 cut(s) 238, 709, 736, 747, 1136
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 8 cut(s) 245, 292, 299, 315, 322, 431, 828, 1105
BstMAI GTCTC 5 cut(s) 27, 129, 421, 575, 762
BstMBI GATC 8 cut(s) 242, 289, 296, 312, 319, 428, 825, 1102
BstMWI GCNNNNNNNGC 5 cut(s) 539, 782, 953, 962, 1170
BstNI CCWGG 1 cut(s) 980
BstNSI RCATGY 2 cut(s) 209, 1184
BstPI GGTNACC 1 cut(s) 797
BstSCI CCNGG 3 cut(s) 420, 421, 978
BstSFI CTRYAG 3 cut(s) 105, 366, 1109
BstV1I GCAGC 8 cut(s) 22, 64, 352, 517, 760, 838, 862, 1171
BstV2I GAAGAC 1 cut(s) 932
BstX2I RGATCY 4 cut(s) 242, 296, 319, 428
BstYI RGATCY 4 cut(s) 242, 296, 319, 428
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 2 cut(s) 69, 392
BtgI CCRYGG 1 cut(s) 282
BtrI CACGTC 1 cut(s) 123
BtsCI GGATG 5 cut(s) 238, 709, 736, 747, 1136
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 146, 375
CaiI CAGNNNCTG 1 cut(s) 527
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 91, 617
Cfr9I CCCGGG 1 cut(s) 421
CseI GACGC 2 cut(s) 44, 744
Csp6I GTAC 2 cut(s) 677, 820
CviAII CATG 7 cut(s) 206, 283, 935, 944, 962, 1084, 1181
CviQI GTAC 2 cut(s) 677, 820
DdeI CTNAG 7 cut(s) 149, 387, 548, 690, 759, 879, 1214
DpnI GATC 8 cut(s) 244, 291, 298, 314, 321, 430, 827, 1104
DpnII GATC 8 cut(s) 242, 289, 296, 312, 319, 428, 825, 1102
Eam1104I CTCTTC 3 cut(s) 202, 429, 693
EarI CTCTTC 3 cut(s) 202, 429, 693
Eco130I CCWWGG 1 cut(s) 282
Eco147I AGGCCT 2 cut(s) 69, 392
Eco31I GGTCTC 1 cut(s) 421
Eco47I GGWCC 2 cut(s) 91, 617
Eco57I CTGAAG 2 cut(s) 453, 504
Eco88I CYCGRG 1 cut(s) 421
Eco91I GGTNACC 1 cut(s) 797
EcoO109I RGGNCCY 1 cut(s) 91
EcoO65I GGTNACC 1 cut(s) 797
EcoRII CCWGG 1 cut(s) 978
EcoT14I CCWWGG 1 cut(s) 282
EcoT22I ATGCAT 3 cut(s) 736, 949, 967
ErhI CCWWGG 1 cut(s) 282
Esp3I CGTCTC 2 cut(s) 129, 762
FaeI CATG 7 cut(s) 209, 286, 938, 947, 965, 1087, 1184
FalI AAGNNNNNCTT 2 cut(s) 502, 534
FatI CATG 7 cut(s) 205, 282, 934, 943, 961, 1083, 1180
Fnu4HI GCNGC 8 cut(s) 36, 53, 366, 531, 774, 852, 876, 1185
FokI GGATG 5 cut(s) 245, 716, 743, 754, 1123
Fsp4HI GCNGC 8 cut(s) 36, 53, 366, 531, 774, 852, 876, 1185
FspBI CTAG 2 cut(s) 264, 834
GlaI GCGC 1 cut(s) 111
GluI GCNGC 8 cut(s) 36, 53, 366, 531, 774, 852, 876, 1185
GsuI CTGGAG 2 cut(s) 689, 1001
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 2 cut(s) 69, 392
HapII CCGG 1 cut(s) 422
HgaI GACGC 2 cut(s) 44, 744
HhaI GCGC 1 cut(s) 112
Hin1II CATG 7 cut(s) 209, 286, 938, 947, 965, 1087, 1184
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 6 cut(s) 467, 524, 546, 664, 671, 1070
HpaII CCGG 1 cut(s) 422
HphI GGTGA 1 cut(s) 809
Hpy166II GTNNAC 1 cut(s) 712
Hpy188I TCNGA 7 cut(s) 60, 168, 294, 433, 478, 523, 952
Hpy188III TCNNGA 3 cut(s) 668, 898, 1122
Hpy8I GTNNAC 1 cut(s) 712
HpyAV CCTTC 2 cut(s) 82, 528
HpyCH4III ACNGT 1 cut(s) 819
HpyCH4IV ACGT 2 cut(s) 122, 1057
HpyF10VI GCNNNNNNNGC 5 cut(s) 539, 782, 953, 962, 1170
HpyF3I CTNAG 7 cut(s) 149, 387, 548, 690, 759, 879, 1214
HpySE526I ACGT 2 cut(s) 122, 1057
Hsp92II CATG 7 cut(s) 209, 286, 938, 947, 965, 1087, 1184
HspAI GCGC 1 cut(s) 110
Kzo9I GATC 8 cut(s) 242, 289, 296, 312, 319, 428, 825, 1102
Lsp1109I GCAGC 8 cut(s) 22, 64, 352, 517, 760, 838, 862, 1171
LweI GCATC 4 cut(s) 469, 721, 956, 1124
MaeI CTAG 2 cut(s) 264, 834
MaeII ACGT 2 cut(s) 122, 1057
MaeIII GTNAC 2 cut(s) 3, 797
MalI GATC 8 cut(s) 244, 291, 298, 314, 321, 430, 827, 1104
MboI GATC 8 cut(s) 242, 289, 296, 312, 319, 428, 825, 1102
MboII GAAGA 8 cut(s) 189, 291, 299, 322, 438, 446, 710, 932
MflI RGATCY 4 cut(s) 242, 296, 319, 428
MluCI AATT 7 cut(s) 81, 259, 397, 885, 923, 957, 987
MlyI GAGTC 4 cut(s) 540, 673, 680, 1079
MmeI TCCRAC 2 cut(s) 1080, 1217
Mph1103I ATGCAT 3 cut(s) 736, 949, 967
MroXI GAANNNNTTC 1 cut(s) 957
MseI TTAA 7 cut(s) 404, 596, 737, 864, 890, 1035, 1053
MslI CAYNNNNRTG 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 1199
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 3 cut(s) 422, 423, 980
Mva1269I GAATGC 1 cut(s) 763
MvaI CCWGG 1 cut(s) 980
MwoI GCNNNNNNNGC 5 cut(s) 539, 782, 953, 962, 1170
NciI CCSGG 2 cut(s) 422, 423
NcoI CCATGG 1 cut(s) 282
NdeII GATC 8 cut(s) 242, 289, 296, 312, 319, 428, 825, 1102
NlaIII CATG 7 cut(s) 209, 286, 938, 947, 965, 1087, 1184
NlaIV GGNNCC 2 cut(s) 93, 165
NmeAIII GCCGAG 1 cut(s) 1199
NmuCI GTSAC 2 cut(s) 3, 797
NsiI ATGCAT 3 cut(s) 736, 949, 967
NspI RCATGY 2 cut(s) 209, 1184
NspV TTCGAA 1 cut(s) 767
OliI CACNNNNGTG 1 cut(s) 135
PceI AGGCCT 2 cut(s) 69, 392
PciI ACATGT 1 cut(s) 205
PctI GAATGC 1 cut(s) 763
PdmI GAANNNNTTC 1 cut(s) 957
PfeI GAWTC 2 cut(s) 467, 524
PkrI GCNGC 8 cut(s) 37, 54, 367, 532, 775, 853, 877, 1186
PleI GAGTC 4 cut(s) 540, 672, 679, 1078
PpsI GAGTC 4 cut(s) 540, 672, 679, 1078
PpuMI RGGWCCY 1 cut(s) 91
PscI ACATGT 1 cut(s) 205
Psp1406I AACGTT 1 cut(s) 1057
Psp5II RGGWCCY 1 cut(s) 91
Psp6I CCWGG 1 cut(s) 978
PspEI GGTNACC 1 cut(s) 797
PspGI CCWGG 1 cut(s) 978
PspN4I GGNNCC 2 cut(s) 93, 165
PspPI GGNCC 2 cut(s) 91, 617
PspPPI RGGWCCY 1 cut(s) 91
PsrI GAACNNNNNNTAC 2 cut(s) 984, 1016
PstI CTGCAG 1 cut(s) 370
PstNI CAGNNNCTG 1 cut(s) 527
PsuI RGATCY 4 cut(s) 242, 296, 319, 428
PvuII CAGCTG 1 cut(s) 1199
RsaI GTAC 2 cut(s) 678, 821
RsaNI GTAC 2 cut(s) 677, 820
RseI CAYNNNNRTG 1 cut(s) 135
SaqAI TTAA 7 cut(s) 404, 596, 737, 864, 890, 1035, 1053
SatI GCNGC 8 cut(s) 36, 53, 366, 531, 774, 852, 876, 1185
Sau3AI GATC 8 cut(s) 242, 289, 296, 312, 319, 428, 825, 1102
Sau96I GGNCC 2 cut(s) 91, 617
ScaI AGTACT 1 cut(s) 821
SchI GAGTC 4 cut(s) 540, 673, 680, 1079
ScrFI CCNGG 3 cut(s) 422, 423, 980
SfaNI GCATC 4 cut(s) 469, 721, 956, 1124
SfcI CTRYAG 3 cut(s) 105, 366, 1109
SfuI TTCGAA 1 cut(s) 767
SinI GGWCC 2 cut(s) 91, 617
SmaI CCCGGG 1 cut(s) 423
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 4 cut(s) 47, 71, 487, 715
SmoI CTYRAG 4 cut(s) 47, 71, 487, 715
Sse9I AATT 7 cut(s) 81, 259, 397, 885, 923, 957, 987
SseBI AGGCCT 2 cut(s) 69, 392
SspMI CTAG 2 cut(s) 264, 834
StuI AGGCCT 2 cut(s) 69, 392
StyD4I CCNGG 3 cut(s) 420, 421, 978
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 1 cut(s) 819
TaiI ACGT 2 cut(s) 125, 1060
TaqI TCGA 2 cut(s) 767, 824
TasI AATT 7 cut(s) 81, 259, 397, 885, 923, 957, 987
TatI WGTACW 1 cut(s) 819
TfiI GAWTC 2 cut(s) 467, 524
Tru1I TTAA 7 cut(s) 404, 596, 737, 864, 890, 1035, 1053
Tru9I TTAA 7 cut(s) 404, 596, 737, 864, 890, 1035, 1053
TscAI CASTG 2 cut(s) 153, 375
TseFI GTSAC 2 cut(s) 3, 797
TseI GCWGC 8 cut(s) 35, 52, 365, 530, 773, 851, 875, 1184
Tsp45I GTSAC 2 cut(s) 3, 797
TspDTI ATGAA 3 cut(s) 932, 950, 1050
TspGWI ACGGA 1 cut(s) 1009
TspMI CCCGGG 1 cut(s) 421
TspRI CASTG 2 cut(s) 153, 375
VpaK11BI GGWCC 2 cut(s) 91, 617
XapI RAATTY 3 cut(s) 259, 397, 885
XceI RCATGY 2 cut(s) 209, 1184
XmaI CCCGGG 1 cut(s) 421
XmnI GAANNNNTTC 1 cut(s) 957
XspI CTAG 2 cut(s) 264, 834
ZrmI AGTACT 1 cut(s) 821
Zsp2I ATGCAT 3 cut(s) 736, 949, 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.