AT5G27030

Topless-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005953
Physical Location & Seq
Reverse (-)
9508718 .. 9515565
6848 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27030.1

Sequence Viewer

Length: 3327 bp
ATGTCGTCGTTGAGTCGAGAGTTGGTGTTTCTGATACTTCAATTTCTGGAGGAAGAGAAATTTAAGGAATCTGTGCACAGGCTTGAGAAAGAGTCAGGATTCTTCTTCAATACCAAGTATTTTGATGAGAAAGTTCTAGCTGGAGAATGGGATGATGTTGAGACGTATTTGTCAGGTTTTACTAAGGTTGATGATAACAGATACTCTATGAAGATTTTCTTTGAAATTAGGAAGCAGAAGTATCTCGAGGCTCTTGATCGGCAAGAGAAGGCAAAAGCAGTTGAGATACTAGTGCAAGATTTGAGAGTTTTCTCCACTTTCAATGAGGAGCTTTACAAAGAAATCACTCAACTCTTAACTTTGCAAAACTTTAGGGAAAATGAGCAGCTTTCAAAATATGGAGACACTAAAACTGCTCGGGGCATAATGCTAGGCGAACTTAAAAAACTAATTGAAGCGAATCCACTTTTTCGTGATAAATTGATGTTCCCTACTTTGAGATCTTCGAGACTGCGGACTCTGATTAATCAAAGCCTCAATTGGCAGCACCAGTTGTGCAAGAATCCTAGGCCAAACCCAGATATTAAAACTCTATTCACAGACCACACTTGCACACTTCCGAATGGTCCTCTCGCACCTTCAGCAGTCAATCAGCCTGTTACTACTTTGACGAAGCCAGCAGCTTATCCGTCACTTGGACCTCATGTTCCCTTTCCACCTGGTCCTGCTGCTGCTAATGCTGGTGCTTTAGCTAGTTGGATGGCTGCTGCCTCTGGTGCTTCTGCTGTCCAAGCTGCCGTTGTTACACCTGCCTTGATGCCTCAACCTCAGAATCAAATGTCAATCTTGAAGCGACCAAGAACACCACCAGCAACTCCAGGTATAGTAGATTATCAGAATCCTGATCATGAACTAATGAAGCGTCTCCGCCCTGCTCCATCTGTGGAGGAGGTGACATATCCTGCTCCTAGGCAGCAAGCTCCATGGTCGCTGGAAGATTTGCCAACAAAGGCAGCTCTGGCGCTGCATCAAGGGTCCACTGTGACAAGCATGGAGTTCTACCCTATGCAAAACACGTTACTTCTTGTTGGATCTGCGACGGGAGAAATCACATTGTGGGAACTTGCCGCTCGAGAGAGGCTGGTTTCAAGGCCATTCAAGATATGGGATATGTCTAATTGCTCACATCAGTTTCAGGCTTTGATAGCTAAAGAAACACCGATATCTGTCACTCGCGTTGCATGGAGTCCGGATGGAAATTTCATTGGGGTTGCGTTTACAAAACATCTAATTCAGTTGTACGCTTTCTCTGGACCTAATGATCTTCGCCAACATACTGAGATTGATGCCCATGTCGGTGCTGTGAACGACTTGGCTTTCGCCAATCCAAACAGACAACTATGTGTAATTACTTGCGGAGATGATAAATTAATCAAGGTATGGGATGTTTCTGGTCGGAAGCATTTTACTTTTGAAGGCCACGACGCTCCAGTTTATTCCATTTGCCCTCATTACAAAGAGAACATTCAGTTCATATTTTCAACCGCCATAGATGGGAAAATAAAAGCCTGGCTTTATGATAATCTGGGTTCCAGAGTTGACTATGATGCTCCCGGTAAATGGTGTACGAGGATGCTTTACAGTGCTGATGGTACTAGATTGTTCTCTTGTGGAACGAGTAAAGATGGAGATTCTTTCCTAGTTGAGTGGAATGAAAGTGAAGGATCAATTAAAAGGACGTATAAAGAGTTTCAGAAAAAGTTGGCAGGTGTGGTTCAGTTCGATACCTCAAAAAACCATTTTCTGGCTGTTGGTGAAGATGGACAAATCAAATTTTGGGATATGAACAACATCAATGTTCTGACTAGCACTGATGCTGAGGGTGGACTTCCGGCTCTTCCCCATTTGAGATTTAACAAGGACGGCAATCTTCTAGCCGTTACTACGGCAGATAACGGATTTAAGATCCTAGCTAATCCAGCTGGTTTCCGATCTCTTCGAGCGATGGAAACTCCAGCTTCTGAAACGATGAGGACTCCAGTTGATTTCAAAGCTGTTCCTGGTGCTCCTGTTGCGTCTGTCAATTGTAAAGTTGAACGAGGCTCTCCTGTTAGACACTCACAAATGCTGAATGGAGTTGATCCCTCAAAGTCGAGAATCGATGACTCAACAGACAAACCAAAATCTTGGCAATTAGCTGAAATCTTGGACCCTTCCCAGTGTTTCCAAGCTACTTTACCCGATACCGCTGGTTCTTCCACAAAGGTTGTTCAGCTTCTGTATACGAATTCCGGTGCTGGAATCTTGGCACTAGGTTCCAATGGTATCCAGAGACTCTGGAAGTGGGTTCCCAATGAGCAGAACCCTAGTGGAAAGGCAACTGCTACTGTTGTTCCTCAGCATTGGCAACCAAACAGTGGTCTTCTTATGACCAATGATGTGTCTGGTGTAAACCTTGAAAACGCTGCCCCATGCATCGCTCTCTCTAAGAACGACTCATATGTCATGTCTGCTGCTGGAGGAAAAGTCTCGTTGTTCAACATGATGACTTTTAAGGTGATGACAACATTCATGCCGCCTCCACCAGCATCAACGTTTTTGGCGTTCCATCCTCAGGACAATAACGTCATCGCCATTGGAATGGAAGACTCTACGATTCACATCTATAATGTCCGAGTGGATGAGGTCAAATCAAAGCTAAAGGGTCACCAGAAACGCATCACAGGCCTAGCATTTTCTACAGCCCTCAACATCTTGGTTTCATCTGGTGCTGATGCACAGATCTGCTTTTGGAGCATTGACACATGGGAGAAGAGGAAATCCGTTGCAATACAAATGCCAGCAGGAAAAGCCGCCAATGGAGACACGCGGGTTCAGTTTCATGTGGATCAGTTGCGTATTCTGGTAGTCCACGAGACACAACTGGCGGTATTTGATGCTTCCAAGATGGAATGTATCCGACAGTGGATTCCACAAGACTCGCTGTCTGCTCCTATATCTTCAGCGGTGTACGCATGCAACAGCCAGCTGATCTACACCACTTTCCGCGATGGTAACATTGGAGTATTTGACGCGGACAGTCTCAGATTAAGATGTCGTATCTCTCCATCCGCCTACTTGCCTCAAGGGAATCAAGGCTTGTCTCCTCTTGTTGTGGCGGCTCACCCGCAAGACCCAAACCAGTTTGCGGTAGGTTTGAATGACGGGTCAGTGAAGATGATGGAACCGACAGAGGGTGAAGGCAAGTGGGGGATGATTCCACCCTCTGAAGCTATCAATTCACCATCCACCACAAGCAACCAAACTCCAGAACAGTTACAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

1108

Amino Acids

122.66

Weight (kDa)

6.78

Isoelectric Point (pI)

39.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 34 2.9e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 5.6e-61 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 2.5e-25 TOPLESS, zinc finger domain
Beta-prop_WDR3_2nd PF25172 346 - 561 4.9e-08 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 348 - 498 1.2e-06 RIG second beta-propeller
Beta-prop_WDR3_1st PF25173 387 - 559 1.1e-13 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 389 - 558 1.6e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 409 - 620 3.3e-21 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 409 - 662 9.4e-22 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 456 - 527 1.9e-07 WDHD1 first WD40 domain
WDR55 PF24796 497 - 652 3.9e-07 WDR55
WD40_Prp19 PF24814 564 - 656 7.8e-06 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 812 - 1027 7.7e-17 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 816 - 941 2.1e-12 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 818 - 934 5.8e-08 WDR55
Beta-prop_WDR3_1st PF25173 821 - 941 2e-16 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 821 - 942 8.6e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 822 - 1026 1.8e-16 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 825 - 941 9.4e-13 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 833 - 935 4e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_IFT140_1st PF23383 836 - 935 4.5e-09 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 837 - 936 1.1e-11 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 842 - 936 1.3e-07 WDR90/POC16, second beta-propeller
WD40_Prp19 PF24814 863 - 1025 2.7e-13 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 873 - 1074 8e-12 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 817, 1757
AasI GACNNNNNNGTC 3 cut(s) 169, 2501, 2624
Acc36I ACCTGC 2 cut(s) 817, 1757
AccB7I CCANNNNNTGG 2 cut(s) 1804, 2417
AccBSI CCGCTC 1 cut(s) 1130
AccI GTMKAC 1 cut(s) 2282
AccII CGCG 4 cut(s) 1236, 2869, 3048, 3074
AccIII TCCGGA 1 cut(s) 1249
AclI AACGTT 1 cut(s) 2594
AclWI GGATC 5 cut(s) 1099, 1732, 1960, 2135, 2895
AcsI RAATTY 4 cut(s) 59, 1258, 1832, 2287
AcuI CTGAAG 3 cut(s) 624, 2985, 3288
AdeI CACNNNGTG 1 cut(s) 1116
AfaI GTAC 4 cut(s) 1301, 1627, 1654, 3011
AfiI CCNNNNNNNGG 8 cut(s) 695, 1554, 1620, 1804, 2417, 2614, 3187, 3233
AflIII ACRYGT 1 cut(s) 1074
AhdI GACNNNNNGTC 1 cut(s) 2983
AhlI ACTAGT 1 cut(s) 289
AjnI CCWGG 4 cut(s) 718, 877, 1568, 2059
AloI GAACNNNNNNTCC 2 cut(s) 690, 722
Alw21I GWGCWC 2 cut(s) 78, 2068
Alw44I GTGCAC 1 cut(s) 74
AlwI GGATC 5 cut(s) 1099, 1732, 1960, 2135, 2895
AlwNI CAGNNNCTG 2 cut(s) 2021, 2278
Ama87I CYCGRG 3 cut(s) 245, 417, 1131
Aor13HI TCCGGA 1 cut(s) 1249
AoxI GGCC 4 cut(s) 569, 1151, 1477, 2725
ApaLI GTGCAC 1 cut(s) 74
ApoI RAATTY 4 cut(s) 59, 1258, 1832, 2287
ArsI GACNNNNNNTTYG 2 cut(s) 1361, 1393
AseI ATTAAT 2 cut(s) 525, 1430
Asp700I GAANNNNTTC 1 cut(s) 215
AspA2I CCTAGG 2 cut(s) 566, 968
AspLEI GCGC 1 cut(s) 1024
AspS9I GGNCC 6 cut(s) 626, 698, 722, 1035, 1313, 2209
AsuC2I CCSGG 1 cut(s) 1614
AsuHPI GGTGA 7 cut(s) 964, 1826, 2569, 2699, 3155, 3248, 3273
AvaI CYCGRG 3 cut(s) 245, 417, 1131
AvaII GGWCC 6 cut(s) 626, 698, 722, 1035, 1313, 2209
AvrII CCTAGG 2 cut(s) 566, 968
AxyI CCTNAGG 1 cut(s) 2613
BaeGI GKGCMC 1 cut(s) 78
BauI CACGAG 1 cut(s) 2912
BbsI GAAGAC 2 cut(s) 2414, 2652
Bbv12I GWGCWC 2 cut(s) 78, 2068
BbvCI CCTCAGC 2 cut(s) 1878, 2397
BceAI ACGGC 4 cut(s) 782, 1922, 1939, 1962
BciT130I CCWGG 4 cut(s) 720, 879, 1570, 2061
BciVI GTATCC 2 cut(s) 2336, 2966
BclI TGATCA 1 cut(s) 904
BcnI CCSGG 1 cut(s) 1614
BcuI ACTAGT 1 cut(s) 289
BfmI CTRYAG 1 cut(s) 2739
BfoI RGCGCY 1 cut(s) 1025
BfuAI ACCTGC 2 cut(s) 817, 1757
BfuI GTATCC 2 cut(s) 2336, 2966
BglII AGATCT 2 cut(s) 500, 2781
BlnI CCTAGG 2 cut(s) 566, 968
Bme1390I CCNGG 5 cut(s) 720, 879, 1570, 1614, 2061
Bme18I GGWCC 6 cut(s) 626, 698, 722, 1035, 1313, 2209
BmeRI GACNNNNNGTC 1 cut(s) 2983
BmeT110I CYCGRG 3 cut(s) 245, 417, 1131
BmgT120I GGNCC 6 cut(s) 626, 698, 722, 1035, 1313, 2209
BmiI GGNNCC 6 cut(s) 1036, 1591, 2211, 2317, 2349, 3225
BmrFI CCNGG 5 cut(s) 720, 879, 1570, 1614, 2061
BmrI ACTGGG 1 cut(s) 2212
BmuI ACTGGG 1 cut(s) 2212
BpiI GAAGAC 2 cut(s) 2414, 2652
BpmI CTGGAG 8 cut(s) 68, 162, 861, 1473, 1998, 2022, 2538, 3291
Bpu10I CCTNAGC 2 cut(s) 1878, 2397
BpuEI CTTGAG 2 cut(s) 104, 3108
BpuMI CCSGG 1 cut(s) 1614
Bsa29I ATCGAT 1 cut(s) 2160
BsaBI GATNNNNATC 1 cut(s) 2660
BsaJI CCNNGG 3 cut(s) 566, 968, 983
BsaWI WCCGGW 2 cut(s) 1249, 2291
BsaXI ACNNNNNCTCC 2 cut(s) 2511, 2541
Bsc4I CCNNNNNNNGG 8 cut(s) 695, 1554, 1620, 1804, 2417, 2614, 3187, 3233
Bse1I ACTGG 6 cut(s) 550, 1490, 2039, 2218, 2928, 3181
Bse21I CCTNAGG 1 cut(s) 2613
Bse8I GATNNNNATC 1 cut(s) 2660
BseAI TCCGGA 1 cut(s) 1249
BseBI CCWGG 4 cut(s) 720, 879, 1570, 2061
BseCI ATCGAT 1 cut(s) 2160
BseDI CCNNGG 3 cut(s) 566, 968, 983
BseJI GATNNNNATC 1 cut(s) 2660
BseLI CCNNNNNNNGG 8 cut(s) 695, 1554, 1620, 1804, 2417, 2614, 3187, 3233
BseMII CTCAG 6 cut(s) 842, 1329, 1869, 2411, 2627, 3097
BseNI ACTGG 6 cut(s) 550, 1490, 2039, 2218, 2928, 3181
BseRI GAGGAG 3 cut(s) 341, 962, 3135
BseSI GKGCMC 1 cut(s) 78
Bsh1236I CGCG 4 cut(s) 1236, 2869, 3048, 3074
BshFI GGCC 4 cut(s) 571, 1153, 1479, 2727
BshVI ATCGAT 1 cut(s) 2160
BsiHKAI GWGCWC 2 cut(s) 78, 2068
BsiHKCI CYCGRG 3 cut(s) 245, 417, 1131
BsiSI CCGG 4 cut(s) 1250, 1614, 1892, 2292
BslI CCNNNNNNNGG 8 cut(s) 695, 1554, 1620, 1804, 2417, 2614, 3187, 3233
BsmBI CGTCTC 2 cut(s) 155, 929
BsnI GGCC 4 cut(s) 571, 1153, 1479, 2727
BsoBI CYCGRG 3 cut(s) 245, 417, 1131
Bsp1286I GDGCHC 2 cut(s) 78, 2068
Bsp13I TCCGGA 1 cut(s) 1249
Bsp19I CCATGG 1 cut(s) 983
BspANI GGCC 4 cut(s) 571, 1153, 1479, 2727
BspCNI CTCAG 6 cut(s) 841, 1330, 1870, 2410, 2626, 3096
BspDI ATCGAT 1 cut(s) 2160
BspEI TCCGGA 1 cut(s) 1249
BspFNI CGCG 4 cut(s) 1236, 2869, 3048, 3074
BspHI TCATGA 1 cut(s) 907
BspLI GGNNCC 6 cut(s) 1036, 1591, 2211, 2317, 2349, 3225
BspMI ACCTGC 2 cut(s) 817, 1757
BspPI GGATC 5 cut(s) 1099, 1732, 1960, 2135, 2895
BspQI GCTCTTC 1 cut(s) 1902
BsrBI CCGCTC 1 cut(s) 1130
BsrI ACTGG 6 cut(s) 550, 1490, 2039, 2218, 2928, 3181
BssECI CCNNGG 3 cut(s) 566, 968, 983
BssNAI GTATAC 1 cut(s) 2283
BssSI CACGAG 1 cut(s) 2912
BssT1I CCWWGG 3 cut(s) 566, 968, 983
Bst1107I GTATAC 1 cut(s) 2283
Bst2BI CACGAG 1 cut(s) 2912
Bst2UI CCWGG 4 cut(s) 720, 879, 1570, 2061
Bst4CI ACNGT 7 cut(s) 1042, 1643, 2389, 2417, 2964, 3080, 3315
Bst6I CTCTTC 4 cut(s) 48, 1902, 2001, 2807
BstC8I GCNNGC 5 cut(s) 678, 978, 2841, 3016, 3026
BstDEI CTNAG 8 cut(s) 183, 828, 1338, 1878, 2397, 2487, 2613, 3083
BstDSI CCRYGG 1 cut(s) 983
BstEII GGTNACC 1 cut(s) 2705
BstFNI CGCG 4 cut(s) 1236, 2869, 3048, 3074
BstH2I RGCGCY 1 cut(s) 1025
BstHHI GCGC 1 cut(s) 1024
BstNI CCWGG 4 cut(s) 720, 879, 1570, 2061
BstNSI RCATGY 1 cut(s) 3018
BstPI GGTNACC 1 cut(s) 2705
BstSCI CCNGG 5 cut(s) 718, 877, 1568, 1612, 2059
BstSFI CTRYAG 1 cut(s) 2739
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 4 cut(s) 1236, 2869, 3048, 3074
BstV2I GAAGAC 2 cut(s) 2414, 2652
BstX2I RGATCY 4 cut(s) 500, 1091, 1965, 2781
BstXI CCANNNNNNTGG 2 cut(s) 2187, 2641
BstYI RGATCY 4 cut(s) 500, 1091, 1965, 2781
BstZ17I GTATAC 1 cut(s) 2283
Bsu15I ATCGAT 1 cut(s) 2160
Bsu36I CCTNAGG 1 cut(s) 2613
BsuI GTATCC 2 cut(s) 2336, 2966
BsuRI GGCC 4 cut(s) 571, 1153, 1479, 2727
BsuTUI ATCGAT 1 cut(s) 2160
BtgI CCRYGG 1 cut(s) 983
BtgZI GCGATG 4 cut(s) 2018, 2461, 2614, 3063
BtsIMutI CAGTG 7 cut(s) 1038, 1648, 1869, 2225, 2422, 2969, 3216
BveI ACCTGC 2 cut(s) 817, 1757
Cac8I GCNNGC 5 cut(s) 678, 978, 2841, 3016, 3026
CaiI CAGNNNCTG 2 cut(s) 2021, 2278
CciI TCATGA 1 cut(s) 907
CfoI GCGC 1 cut(s) 1024
Cfr13I GGNCC 6 cut(s) 626, 698, 722, 1035, 1313, 2209
ClaI ATCGAT 1 cut(s) 2160
CseI GACGC 4 cut(s) 911, 1493, 2064, 3080
CsiI ACCWGGT 1 cut(s) 718
Csp6I GTAC 4 cut(s) 1300, 1626, 1653, 3010
CspCI CAANNNNNGTGG 2 cut(s) 2248, 2283
CviQI GTAC 4 cut(s) 1300, 1626, 1653, 3010
DdeI CTNAG 8 cut(s) 183, 828, 1338, 1878, 2397, 2487, 2613, 3083
DraIII CACNNNGTG 1 cut(s) 1116
DrdI GACNNNNNNGTC 3 cut(s) 169, 2501, 2624
DriI GACNNNNNGTC 1 cut(s) 2983
DseDI GACNNNNNNGTC 3 cut(s) 169, 2501, 2624
Eam1104I CTCTTC 4 cut(s) 48, 1902, 2001, 2807
Eam1105I GACNNNNNGTC 1 cut(s) 2983
EarI CTCTTC 4 cut(s) 48, 1902, 2001, 2807
EciI GGCGGA 2 cut(s) 917, 3100
Eco130I CCWWGG 3 cut(s) 566, 968, 983
Eco147I AGGCCT 1 cut(s) 2727
Eco32I GATATC 1 cut(s) 1224
Eco47I GGWCC 6 cut(s) 626, 698, 722, 1035, 1313, 2209
Eco57I CTGAAG 3 cut(s) 624, 2985, 3288
Eco81I CCTNAGG 1 cut(s) 2613
Eco88I CYCGRG 3 cut(s) 245, 417, 1131
Eco91I GGTNACC 1 cut(s) 2705
EcoO65I GGTNACC 1 cut(s) 2705
EcoRI GAATTC 1 cut(s) 2287
EcoRII CCWGG 4 cut(s) 718, 877, 1568, 2059
EcoRV GATATC 1 cut(s) 1224
EcoT14I CCWWGG 3 cut(s) 566, 968, 983
EcoT22I ATGCAT 1 cut(s) 2477
ErhI CCWWGG 3 cut(s) 566, 968, 983
Esp3I CGTCTC 2 cut(s) 155, 929
FalI AAGNNNNNCTT 4 cut(s) 203, 235, 1557, 1589
FauI CCCGC 2 cut(s) 2862, 3174
FauNDI CATATG 1 cut(s) 2500
FbaI TGATCA 1 cut(s) 904
FblI GTMKAC 1 cut(s) 2282
GlaI GCGC 1 cut(s) 1023
GsuI CTGGAG 8 cut(s) 68, 162, 861, 1473, 1998, 2022, 2538, 3291
HaeII RGCGCY 1 cut(s) 1025
HaeIII GGCC 4 cut(s) 571, 1153, 1479, 2727
HapII CCGG 4 cut(s) 1250, 1614, 1892, 2292
HgaI GACGC 4 cut(s) 911, 1493, 2064, 3080
HhaI GCGC 1 cut(s) 1024
Hin6I GCGC 1 cut(s) 1022
HinP1I GCGC 1 cut(s) 1022
HincII GTYRAC 1 cut(s) 1600
HindII GTYRAC 1 cut(s) 1600
HpaII CCGG 4 cut(s) 1250, 1614, 1892, 2292
HphI GGTGA 7 cut(s) 964, 1826, 2569, 2699, 3155, 3248, 3273
Hpy99I CGWCG 3 cut(s) 10, 1102, 1487
HpyAV CCTTC 6 cut(s) 262, 648, 1469, 1715, 2223, 3233
HpyCH4III ACNGT 7 cut(s) 1042, 1643, 2389, 2417, 2964, 3080, 3315
HpyCH4IV ACGT 5 cut(s) 164, 1076, 1739, 2594, 2625
HpyF3I CTNAG 8 cut(s) 183, 828, 1338, 1878, 2397, 2487, 2613, 3083
HpySE526I ACGT 5 cut(s) 164, 1076, 1739, 2594, 2625
HspAI GCGC 1 cut(s) 1022
Kpn2I TCCGGA 1 cut(s) 1249
Ksp22I TGATCA 1 cut(s) 904
LguI GCTCTTC 1 cut(s) 1902
LmnI GCTCC 9 cut(s) 328, 940, 970, 985, 1492, 1615, 2071, 2793, 2995
MabI ACCWGGT 1 cut(s) 718
MaeII ACGT 5 cut(s) 164, 1076, 1739, 2594, 2625
MbiI CCGCTC 1 cut(s) 1130
MfeI CAATTG 2 cut(s) 538, 2083
MflI RGATCY 4 cut(s) 500, 1091, 1965, 2781
MhlI GDGCHC 2 cut(s) 78, 2068
MmeI TCCRAC 4 cut(s) 737, 1069, 1436, 2983
Mph1103I ATGCAT 1 cut(s) 2477
MroI TCCGGA 1 cut(s) 1249
MroXI GAANNNNTTC 1 cut(s) 215
MslI CAYNNNNRTG 2 cut(s) 1356, 2639
MspA1I CMGCKG 4 cut(s) 1982, 2249, 3005, 3028
MspI CCGG 4 cut(s) 1250, 1614, 1892, 2292
MspR9I CCNGG 5 cut(s) 720, 879, 1570, 1614, 2061
MunI CAATTG 2 cut(s) 538, 2083
MvaI CCWGG 4 cut(s) 720, 879, 1570, 2061
MvnI CGCG 4 cut(s) 1236, 2869, 3048, 3074
NciI CCSGG 1 cut(s) 1614
NcoI CCATGG 1 cut(s) 983
NdeI CATATG 1 cut(s) 2500
NlaIV GGNNCC 6 cut(s) 1036, 1591, 2211, 2317, 2349, 3225
NmuCI GTSAC 5 cut(s) 690, 952, 1042, 1228, 2705
NsiI ATGCAT 1 cut(s) 2477
NspI RCATGY 1 cut(s) 3018
PaeI GCATGC 1 cut(s) 3018
PaeR7I CTCGAG 2 cut(s) 245, 1131
PagI TCATGA 1 cut(s) 907
PaqCI CACCTGC 2 cut(s) 817, 1757
PceI AGGCCT 1 cut(s) 2727
PciSI GCTCTTC 1 cut(s) 1902
PcsI WCGNNNNNNNCGW 1 cut(s) 2600
PdmI GAANNNNTTC 1 cut(s) 215
PflMI CCANNNNNTGG 2 cut(s) 1804, 2417
PshBI ATTAAT 2 cut(s) 525, 1430
Psp1406I AACGTT 1 cut(s) 2594
Psp6I CCWGG 4 cut(s) 718, 877, 1568, 2059
PspEI GGTNACC 1 cut(s) 2705
PspGI CCWGG 4 cut(s) 718, 877, 1568, 2059
PspN4I GGNNCC 6 cut(s) 1036, 1591, 2211, 2317, 2349, 3225
PspPI GGNCC 6 cut(s) 626, 698, 722, 1035, 1313, 2209
PstNI CAGNNNCTG 2 cut(s) 2021, 2278
PsuI RGATCY 4 cut(s) 500, 1091, 1965, 2781
PvuII CAGCTG 2 cut(s) 1982, 3028
RsaI GTAC 4 cut(s) 1301, 1627, 1654, 3011
RsaNI GTAC 4 cut(s) 1300, 1626, 1653, 3010
RseI CAYNNNNRTG 2 cut(s) 1356, 2639
SapI GCTCTTC 1 cut(s) 1902
Sau96I GGNCC 6 cut(s) 626, 698, 722, 1035, 1313, 2209
ScrFI CCNGG 5 cut(s) 720, 879, 1570, 1614, 2061
SduI GDGCHC 2 cut(s) 78, 2068
SexAI ACCWGGT 1 cut(s) 718
SfcI CTRYAG 1 cut(s) 2739
Sfr274I CTCGAG 2 cut(s) 245, 1131
SinI GGWCC 6 cut(s) 626, 698, 722, 1035, 1313, 2209
SlaI CTCGAG 2 cut(s) 245, 1131
SmiMI CAYNNNNRTG 2 cut(s) 1356, 2639
SmlI CTYRAG 4 cut(s) 83, 245, 1131, 3123
SmoI CTYRAG 4 cut(s) 83, 245, 1131, 3123
SpeI ACTAGT 1 cut(s) 289
SphI GCATGC 1 cut(s) 3018
SseBI AGGCCT 1 cut(s) 2727
StuI AGGCCT 1 cut(s) 2727
StyD4I CCNGG 5 cut(s) 718, 877, 1568, 1612, 2059
StyI CCWWGG 3 cut(s) 566, 968, 983
TaaI ACNGT 7 cut(s) 1042, 1643, 2389, 2417, 2964, 3080, 3315
TaiI ACGT 5 cut(s) 167, 1079, 1742, 2597, 2628
TaqI TCGA 8 cut(s) 16, 246, 506, 1132, 1782, 1999, 2153, 2160
TauI GCSGC 4 cut(s) 1130, 2578, 2855, 3161
TscAI CASTG 7 cut(s) 1045, 1648, 1876, 2225, 2422, 2969, 3216
TseFI GTSAC 5 cut(s) 690, 952, 1042, 1228, 2705
Tsp45I GTSAC 5 cut(s) 690, 952, 1042, 1228, 2705
TspGWI ACGGA 3 cut(s) 678, 1971, 2812
TspRI CASTG 7 cut(s) 1045, 1648, 1876, 2225, 2422, 2969, 3216
Van91I CCANNNNNTGG 2 cut(s) 1804, 2417
VneI GTGCAC 1 cut(s) 74
VpaK11BI GGWCC 6 cut(s) 626, 698, 722, 1035, 1313, 2209
VspI ATTAAT 2 cut(s) 525, 1430
XapI RAATTY 4 cut(s) 59, 1258, 1832, 2287
XceI RCATGY 1 cut(s) 3018
XcmI CCANNNNNNNNNTGG 1 cut(s) 1161
XhoI CTCGAG 2 cut(s) 245, 1131
XmaJI CCTAGG 2 cut(s) 566, 968
XmiI GTMKAC 1 cut(s) 2282
XmnI GAANNNNTTC 1 cut(s) 215
Zsp2I ATGCAT 1 cut(s) 2477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.