Rmu_sc0023184.1_g000002

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0023184.1
OG0005953
Physical Location & Seq
Forward (+)
10310 .. 12904
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0023184.1_g000002.1.cds

Sequence Viewer

Length: 1197 bp
atgaaattacatttgcaatgtcgtagtgtacgtaagattctattttttgatttagcagttccaagcctaaagcagccaagaacagctctggaaacttctggtggggttgatccttctagtactggttttactgctattgaccggatggcgaaagaactgcaacatgttcgatcgaacttggagggtacctttcaaatttctagactgcttcaaacttcctggttggtggagaacctaccgagaaatgtggcttatagcatgcgtcaaggatctacagtcacaagcatggattttcatccttatcaccatgtattgctcctaggtgtgatgagcacacttgccaatgaagacacatctatatctgtgagccgtgttacatggagtccagatgggaacttttttggagttgcatttactaaaaatttgattcacttgtatgctttttctggacttagtgatctatgccggtatttggaggtacatgttatcacggttgacgcccatgatggtggtgtgaatgacttggcttttgattatcgaaacaacgaactgtgtgttatcacatgtggagatgacaagctaataaagtacatatattcaacttctactgatgggaagataaagacctggatgtacgacaatgggggtcctagatctggctgtgatgctcctggcaactggtgtactacattgcttttcagtccggatggtaaccgaatgttctcttgtggaacaaataaagagggagacacttttctagttcaatggagcgaaaatgaaggaataattaaagaaggagcaaaaaagagaatttattctgggtttggtaagaaatctgaaggggttgtgcattttgacataacgaaaaagaattttttggctgtgggggaagatagccagatcaagtattgggatatggaccatgatgatgtgctgactagcacggacgcccagggtgaactgccggtaagtcgtcctcgcttgagattcaacaaggaaggtagtcttcttgctgtcacaacagcacatgagggattcatgatacttgcaaatggaatcggacgcctgagagttcatggagtcgctgcaacttatcatgacaggtcgaaagatcctactgaatccactgcaaacaaggttccagtgatttacttggcactttgcatctggctcatcttcggttgcaaatgtcagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

398

Amino Acids

44.35

Weight (kDa)

6.67

Isoelectric Point (pI)

31.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 185
AccB1I GGYRCC 1 cut(s) 185
AccIII TCCGGA 1 cut(s) 703
AclWI GGATC 3 cut(s) 104, 277, 1106
AcsI RAATTY 4 cut(s) 195, 421, 810, 871
AcuI CTGAAG 1 cut(s) 858
AcyI GRCGYC 3 cut(s) 498, 948, 1063
AfaI GTAC 7 cut(s) 30, 121, 187, 480, 590, 635, 685
AfiI CCNNNNNNNGG 2 cut(s) 472, 656
AflIII ACRYGT 3 cut(s) 163, 481, 563
AgsI TTSAA 5 cut(s) 194, 212, 600, 764, 991
AjnI CCWGG 4 cut(s) 218, 626, 670, 951
AluBI AGCT 2 cut(s) 86, 580
AluI AGCT 2 cut(s) 86, 580
Alw21I GWGCWC 1 cut(s) 335
Alw26I GTCTC 1 cut(s) 741
AlwI GGATC 3 cut(s) 104, 277, 1106
Aor13HI TCCGGA 1 cut(s) 703
ApeKI GCWGC 2 cut(s) 73, 1085
ApoI RAATTY 4 cut(s) 195, 421, 810, 871
ArsI GACNNNNNNTTYG 2 cut(s) 512, 544
Asp700I GAANNNNTTC 1 cut(s) 814
Asp718I GGTACC 1 cut(s) 185
AspA2I CCTAGG 1 cut(s) 319
AspS9I GGNCC 2 cut(s) 647, 919
AsuHPI GGTGA 2 cut(s) 296, 968
AvaII GGWCC 2 cut(s) 647, 919
AvrII CCTAGG 1 cut(s) 319
BanI GGYRCC 1 cut(s) 185
BbsI GAAGAC 2 cut(s) 354, 998
Bbv12I GWGCWC 1 cut(s) 335
BbvI GCAGC 2 cut(s) 85, 1072
BccI CCATC 5 cut(s) 139, 383, 500, 605, 701
BceAI ACGGC 1 cut(s) 354
BcgI CGANNNNNNTGC 4 cut(s) 139, 149, 173, 183
BciT130I CCWGG 4 cut(s) 220, 628, 672, 953
BcoDI GTCTC 1 cut(s) 741
BfaI CTAG 6 cut(s) 117, 201, 320, 651, 758, 939
BfmI CTRYAG 1 cut(s) 273
BglII AGATCT 1 cut(s) 653
BisI GCNGC 2 cut(s) 74, 1086
BlnI CCTAGG 1 cut(s) 319
BlsI GCNGC 2 cut(s) 75, 1087
BmcAI AGTACT 1 cut(s) 121
Bme1390I CCNGG 4 cut(s) 220, 628, 672, 953
Bme18I GGWCC 2 cut(s) 647, 919
BmgT120I GGNCC 2 cut(s) 647, 919
BmiI GGNNCC 3 cut(s) 187, 648, 1140
BmrFI CCNGG 4 cut(s) 220, 628, 672, 953
BmsI GCATC 2 cut(s) 655, 1173
BpiI GAAGAC 2 cut(s) 354, 998
BpuEI CTTGAG 1 cut(s) 1003
BsaAI YACGTR 1 cut(s) 32
BsaBI GATNNNNATC 1 cut(s) 294
BsaHI GRCGYC 3 cut(s) 498, 948, 1063
BsaJI CCNNGG 3 cut(s) 319, 951, 952
BsaWI WCCGGW 2 cut(s) 141, 703
Bsc4I CCNNNNNNNGG 2 cut(s) 472, 656
Bse118I RCCGGY 2 cut(s) 465, 964
Bse1I ACTGG 3 cut(s) 127, 683, 1142
Bse3DI GCAATG 2 cut(s) 23, 689
Bse8I GATNNNNATC 1 cut(s) 294
BseAI TCCGGA 1 cut(s) 703
BseBI CCWGG 4 cut(s) 220, 628, 672, 953
BseDI CCNNGG 3 cut(s) 319, 951, 952
BseGI GGATG 4 cut(s) 150, 295, 636, 712
BseJI GATNNNNATC 1 cut(s) 294
BseLI CCNNNNNNNGG 2 cut(s) 472, 656
BseMI GCAATG 2 cut(s) 23, 689
BseMII CTCAG 1 cut(s) 1058
BseNI ACTGG 3 cut(s) 127, 683, 1142
BseXI GCAGC 2 cut(s) 85, 1072
Bsh1285I CGRYCG 1 cut(s) 173
BshNI GGYRCC 1 cut(s) 185
BsiEI CGRYCG 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 335
BsiSI CCGG 4 cut(s) 142, 466, 704, 965
BslI CCNNNNNNNGG 2 cut(s) 472, 656
BsmAI GTCTC 1 cut(s) 741
Bsp1286I GDGCHC 1 cut(s) 335
Bsp13I TCCGGA 1 cut(s) 703
Bsp143I GATC 7 cut(s) 109, 170, 269, 457, 653, 900, 1111
BspCNI CTCAG 1 cut(s) 1059
BspEI TCCGGA 1 cut(s) 703
BspHI TCATGA 2 cut(s) 1038, 1096
BspLI GGNNCC 3 cut(s) 187, 648, 1140
BspPI GGATC 3 cut(s) 104, 277, 1106
BspT107I GGYRCC 1 cut(s) 185
BsrDI GCAATG 2 cut(s) 23, 689
BsrFI RCCGGY 2 cut(s) 465, 964
BsrI ACTGG 3 cut(s) 127, 683, 1142
BssAI RCCGGY 2 cut(s) 465, 964
BssECI CCNNGG 3 cut(s) 319, 951, 952
BssMI GATC 7 cut(s) 109, 170, 269, 457, 653, 900, 1111
BssNI GRCGYC 3 cut(s) 498, 948, 1063
BssT1I CCWWGG 1 cut(s) 319
Bst2UI CCWGG 4 cut(s) 220, 628, 672, 953
Bst4CI ACNGT 3 cut(s) 277, 493, 552
BstACI GRCGYC 3 cut(s) 498, 948, 1063
BstBAI YACGTR 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 260
BstDEI CTNAG 2 cut(s) 452, 1067
BstEII GGTNACC 1 cut(s) 710
BstF5I GGATG 4 cut(s) 150, 295, 636, 712
BstKTI GATC 7 cut(s) 112, 173, 272, 460, 656, 903, 1114
BstMAI GTCTC 1 cut(s) 741
BstMBI GATC 7 cut(s) 109, 170, 269, 457, 653, 900, 1111
BstMCI CGRYCG 1 cut(s) 173
BstNI CCWGG 4 cut(s) 220, 628, 672, 953
BstNSI RCATGY 4 cut(s) 167, 262, 485, 567
BstPI GGTNACC 1 cut(s) 710
BstSCI CCNGG 4 cut(s) 218, 626, 670, 951
BstSFI CTRYAG 1 cut(s) 273
BstSNI TACGTA 1 cut(s) 32
BstV1I GCAGC 2 cut(s) 85, 1072
BstV2I GAAGAC 2 cut(s) 354, 998
BstX2I RGATCY 3 cut(s) 269, 653, 1111
BstXI CCANNNNNNTGG 1 cut(s) 509
BstYI RGATCY 3 cut(s) 269, 653, 1111
BtsCI GGATG 4 cut(s) 150, 295, 636, 712
BtsI GCAGTG 1 cut(s) 1125
BtsIMutI CAGTG 2 cut(s) 1125, 1149
Cac8I GCNNGC 1 cut(s) 260
CciI TCATGA 2 cut(s) 1038, 1096
Cfr10I RCCGGY 2 cut(s) 465, 964
Cfr13I GGNCC 2 cut(s) 647, 919
CseI GACGC 4 cut(s) 251, 506, 956, 1071
Csp6I GTAC 7 cut(s) 29, 120, 186, 479, 589, 634, 684
CviQI GTAC 7 cut(s) 29, 120, 186, 479, 589, 634, 684
DdeI CTNAG 2 cut(s) 452, 1067
DpnI GATC 7 cut(s) 111, 172, 271, 459, 655, 902, 1113
DpnII GATC 7 cut(s) 109, 170, 269, 457, 653, 900, 1111
Eco105I TACGTA 1 cut(s) 32
Eco130I CCWWGG 1 cut(s) 319
Eco47I GGWCC 2 cut(s) 647, 919
Eco57I CTGAAG 1 cut(s) 858
Eco91I GGTNACC 1 cut(s) 710
EcoO109I RGGNCCY 1 cut(s) 647
EcoO65I GGTNACC 1 cut(s) 710
EcoRII CCWGG 4 cut(s) 218, 626, 670, 951
EcoT14I CCWWGG 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 319
FalI AAGNNNNNCTT 2 cut(s) 990, 1022
Fnu4HI GCNGC 2 cut(s) 74, 1086
FokI GGATG 4 cut(s) 157, 282, 643, 719
Fsp4HI GCNGC 2 cut(s) 74, 1086
FspBI CTAG 6 cut(s) 117, 201, 320, 651, 758, 939
GluI GCNGC 2 cut(s) 74, 1086
HapII CCGG 4 cut(s) 142, 466, 704, 965
HgaI GACGC 4 cut(s) 251, 506, 956, 1071
Hin1I GRCGYC 3 cut(s) 498, 948, 1063
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 8 cut(s) 37, 382, 427, 987, 1035, 1056, 1080, 1121
HpaII CCGG 4 cut(s) 142, 466, 704, 965
HphI GGTGA 2 cut(s) 296, 968
Hpy166II GTNNAC 4 cut(s) 29, 496, 684, 959
Hpy188I TCNGA 2 cut(s) 838, 1061
Hpy188III TCNNGA 7 cut(s) 89, 201, 386, 447, 704, 1039, 1097
Hpy8I GTNNAC 4 cut(s) 29, 496, 684, 959
HpyAV CCTTC 5 cut(s) 123, 773, 788, 833, 992
HpyCH4III ACNGT 3 cut(s) 277, 493, 552
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 9 cut(s) 16, 160, 410, 850, 1049, 1088, 1130, 1164, 1185
HpyF3I CTNAG 2 cut(s) 452, 1067
HpySE526I ACGT 1 cut(s) 31
Hsp92I GRCGYC 3 cut(s) 498, 948, 1063
Kpn2I TCCGGA 1 cut(s) 703
KpnI GGTACC 1 cut(s) 189
Kzo9I GATC 7 cut(s) 109, 170, 269, 457, 653, 900, 1111
LmnI GCTCC 4 cut(s) 321, 673, 768, 797
Lsp1109I GCAGC 2 cut(s) 85, 1072
LweI GCATC 2 cut(s) 655, 1173
MaeI CTAG 6 cut(s) 117, 201, 320, 651, 758, 939
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 4 cut(s) 277, 373, 710, 1015
MalI GATC 7 cut(s) 111, 172, 271, 459, 655, 902, 1113
MboI GATC 7 cut(s) 109, 170, 269, 457, 653, 900, 1111
MboII GAAGA 5 cut(s) 359, 628, 902, 998, 1168
MflI RGATCY 3 cut(s) 269, 653, 1111
MhlI GDGCHC 1 cut(s) 335
MluCI AATT 6 cut(s) 5, 195, 421, 786, 810, 871
MlyI GAGTC 2 cut(s) 391, 1089
MnlI CCTC 5 cut(s) 175, 469, 736, 987, 1024
MroI TCCGGA 1 cut(s) 703
MroXI GAANNNNTTC 1 cut(s) 814
MseI TTAA 1 cut(s) 789
MslI CAYNNNNRTG 4 cut(s) 284, 435, 507, 927
MspI CCGG 4 cut(s) 142, 466, 704, 965
MspR9I CCNGG 4 cut(s) 220, 628, 672, 953
MvaI CCWGG 4 cut(s) 220, 628, 672, 953
NdeII GATC 7 cut(s) 109, 170, 269, 457, 653, 900, 1111
NlaIV GGNNCC 3 cut(s) 187, 648, 1140
NmuCI GTSAC 2 cut(s) 277, 1015
NspI RCATGY 4 cut(s) 167, 262, 485, 567
PaeI GCATGC 1 cut(s) 262
PagI TCATGA 2 cut(s) 1038, 1096
PasI CCCWGGG 1 cut(s) 952
PciI ACATGT 3 cut(s) 163, 481, 563
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PdmI GAANNNNTTC 1 cut(s) 814
PfeI GAWTC 6 cut(s) 37, 427, 987, 1035, 1056, 1121
PkrI GCNGC 2 cut(s) 75, 1087
Ple19I CGATCG 1 cut(s) 173
PleI GAGTC 2 cut(s) 390, 1088
PpsI GAGTC 2 cut(s) 390, 1088
Ppu21I YACGTR 1 cut(s) 32
PpuMI RGGWCCY 1 cut(s) 647
PscI ACATGT 3 cut(s) 163, 481, 563
Psp5II RGGWCCY 1 cut(s) 647
Psp6I CCWGG 4 cut(s) 218, 626, 670, 951
PspEI GGTNACC 1 cut(s) 710
PspGI CCWGG 4 cut(s) 218, 626, 670, 951
PspN4I GGNNCC 3 cut(s) 187, 648, 1140
PspPI GGNCC 2 cut(s) 647, 919
PspPPI RGGWCCY 1 cut(s) 647
PsuI RGATCY 3 cut(s) 269, 653, 1111
PvuI CGATCG 1 cut(s) 173
RsaI GTAC 7 cut(s) 30, 121, 187, 480, 590, 635, 685
RsaNI GTAC 7 cut(s) 29, 120, 186, 479, 589, 634, 684
RseI CAYNNNNRTG 4 cut(s) 284, 435, 507, 927
SaqAI TTAA 1 cut(s) 789
SatI GCNGC 2 cut(s) 74, 1086
Sau3AI GATC 7 cut(s) 109, 170, 269, 457, 653, 900, 1111
Sau96I GGNCC 2 cut(s) 647, 919
ScaI AGTACT 1 cut(s) 121
SchI GAGTC 2 cut(s) 391, 1089
ScrFI CCNGG 4 cut(s) 220, 628, 672, 953
SduI GDGCHC 1 cut(s) 335
SfaNI GCATC 2 cut(s) 655, 1173
SfcI CTRYAG 1 cut(s) 273
SinI GGWCC 2 cut(s) 647, 919
SmiMI CAYNNNNRTG 4 cut(s) 284, 435, 507, 927
SmlI CTYRAG 1 cut(s) 982
SmoI CTYRAG 1 cut(s) 982
SnaBI TACGTA 1 cut(s) 32
SphI GCATGC 1 cut(s) 262
Sse9I AATT 6 cut(s) 5, 195, 421, 786, 810, 871
SspMI CTAG 6 cut(s) 117, 201, 320, 651, 758, 939
StyD4I CCNGG 4 cut(s) 218, 626, 670, 951
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 3 cut(s) 277, 493, 552
TaiI ACGT 1 cut(s) 34
TaqI TCGA 4 cut(s) 169, 173, 538, 1106
TasI AATT 6 cut(s) 5, 195, 421, 786, 810, 871
TatI WGTACW 3 cut(s) 119, 588, 683
TfiI GAWTC 6 cut(s) 37, 427, 987, 1035, 1056, 1121
Tru1I TTAA 1 cut(s) 789
Tru9I TTAA 1 cut(s) 789
TscAI CASTG 2 cut(s) 1132, 1149
TseFI GTSAC 2 cut(s) 277, 1015
TseI GCWGC 2 cut(s) 73, 1085
Tsp45I GTSAC 2 cut(s) 277, 1015
TspDTI ATGAA 6 cut(s) 17, 284, 360, 792, 1027, 1064
TspGWI ACGGA 1 cut(s) 959
TspRI CASTG 2 cut(s) 1132, 1149
VpaK11BI GGWCC 2 cut(s) 647, 919
XapI RAATTY 4 cut(s) 195, 421, 810, 871
XbaI TCTAGA 1 cut(s) 200
XceI RCATGY 4 cut(s) 167, 262, 485, 567
XmaJI CCTAGG 1 cut(s) 319
XmnI GAANNNNTTC 1 cut(s) 814
XspI CTAG 6 cut(s) 117, 201, 320, 651, 758, 939
ZrmI AGTACT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.