RLG00000025297

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005953
Physical Location & Seq
Reverse (-)
43675228 .. 43683751
8524 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025297

Sequence Viewer

Length: 3303 bp
ATGTCGAATTTGTGCAGGGAATTGGTGTTACTGGTCCTCCAATTCCTTGACGAGGAGAAGTTCAGAGGGGCCATGCATAAACTTGAGCAAGAATCAGGGTTCTTTTTCAACATGAAGTACTTTGAGGAGAAGATTTGGACTGGAGAATGGGAAGAAGTTGAAAAGTATTTATCAGGATTTACGAAACTCACTGACAATCGATTTTCACTGAAAATAATGTTTGAAATATCAAAGCAGAAATATCTGGAAGCCCTCGATCGGAACGAGAAGGTTGGGGCCATTGACATCTTAGTCAATGATCTCCAGAAGTTTGCTCCTTATAATCAAGAACTTTACAAGGAGATCACACAGCTTTTAACCCTCCGAAACTTTCGGGAAAATGCACATCTCACTCAGTACGGCGACACCAGATCAGCTCGTCATAGAATGGTGGTAGAGCTCAAAAAGCTCATAAAAGCAAACCCTCTATTGAAGGATAAGCTTTATTTTCCTTCTGTGAGGTCAGCCAGACTGCGCACTCTCATTAATCAGAGTCTTAACTGGCAGCACCAGCAATGCAAGGACCCGGTTCCGAATCCAGAAATAAAAACTCTGTTCATAGATCATTACTGTTCACCACCACTAACTGGTCCTCTTGCTCTAGCAAATGTCAATCAGCCATTTCCTGCAATTGAATTTCCTGCTCCTTTTCCCTCAATGGAAGGAGCTATTATTCTATTTTTTGATTTAGCAGTTCCAAGCCTGAAGCGGCCAAGAACAGCTCTGGAAACTTCTGGTGGGGTTGATCCTTCTAGTACTGGTTTTACTGCTATTGACCGGATGGCGAAAGAACTGCAACATGTTCGATCGAACTTGGAGGGTACCTTTCAAACTTCTAGACTGCTTCAAACTTCCTGGTTGGTGGAGAACCTACCAAGAAATGTGGCTTATAGCATGCGTCAAGGATCTACAGTCACAAGCATGGATTTTCATCCTTATCACCATGCATTGCTCCTAGTTGGTGCTGGCAATGGTGAAATTAGTCTTTGGGAACTTGGAACTCGGCAAAGGGTGGCTTCGCAGCCATTCAGAATATGGAATACGGCGGCACGTTCATCAGAACTTCAGAGCACACTTGCCAATGAAGACACATCTATATCTGTGAGCCGTGTTACATGGAGTCCAGATGGGAACTTTTTTGGAGTTGCATTTACTAAAAATTTGATTCACTTGTATGCTCTTTCTGGAATAAGTGATCTATGCCGGTATTTGGAGGTTGACGCCCATGGTGGTGGTGTGAATGACTTGGCTTTTGATTATCGAAACAACGAACTGTGTGTTATCACATGTGGAGATGACAAGCTAATAAAGGTATGGGATATAGCAGGACGAAACTTATACAACTTTGATGGTCATGAAGCACCAGTCTATTCAGTGTGTCCTTATCATAACAAGGACGTTCAGTACATATATTCAACTTCTACTGATGGGAAGATAAAGACCTGGATGTACGGCAATGGGGGTCCTAGATCTGGCTGTGATGCTCCTGGCAACTGGTGTACGACATTGCTTTTCAGTCCGGATGGTAACCGAATGTTCTCTTGTGGAACAAGTAAAGAGGGAGACACTTTTCTAGTTCAATGGAGCGAAAATAAAGGAATAATTAAAGAAGGTGCAAAAAAGAGAATTTATTCTGGGTTCGGTAAGAAATCTGAAGGGGTTGTGCATTTTGACATAACGAAAAAGCATTTTTTGGCTGTGGGTGAAGATAGCCAGATCAAGTATTGGGATATGGACCATGATGATGTTCTGACTAGCACGGACGCCCAGGGTGAACTGCCGAGTCGTCCTCACTTGAGATTCAACAAGGAAGGAAGTCTTCTTGCTGTCACAACGGCACATGAGGGATTCATGATACTTGCAAATGGAATCGGACGTCTGAGAGTTCATGGAGTCACTGCAACTTATCGTGACAGGTCGAAAGATCCTACTGAATCCACTGCAAACAAGGCATCTGGCTCATCTTCGGTTGCAAATGTCAGTGAGGCCAATAGGAAATCTGACAGGGGCTCTCCTGCCAGGTCTACAACATCTCAGAATGGAGCTAATAATGGAGCTAACAGCGAGGGTAGAAGTTCCAAGAAACGGAAAACTGTGGAGGAGGTAATCAGTAAATCCAAACCTTGGCAGTTGACTGACATTCGGGATCCTGTAAGGTGTCGTTTAGGTGTTATGCCTGAAACAGATAGTTCCTGCGAGGTTGTTCGACTCCTATATACAAGTAACGGTACTGCCATCTTGGCACTCGGGTCAAATGGTGTACAGAGGCTGTGGAGGTGGCCCCGTACTAGAGGTCGAATTTCAAAGGCCAGCGCCAGTGTTGTTCCGCAGCTGGTGAAAGGGGACAATGGTCCTGTTATGGCCAATGATGTCTCAGGTGTCAACCTTGAAGAGGCGGTTCCCTGCATAGCGCTCTCAAAGAATGATGCGTATGTAATGTCAGCTTATGGTGGAAGGATCAAATTATTCAACATTTATAATTTGGCGGTAATGATAACTTTCAGGGAACTTCCCCCTGCACCAACTGAAATTGCATTCAATCCTCGGGATAATAATATCATGGCCATTGGAATGCAAGATTGCACCATACAGATTTTCTTTTGTAGACTTAATGAGGTAAGAGCAATCTTGAAGTTTCACAAGAAGCGGATAACTGGTTTAGTTTTCTCGTTCTTTCTTGGTATACTCGTTTCAGCTAGTGCCGACGGTCAAATATGTGTGTGGAGCATTGATCAATGGGAGAAGAAACAATCTGTTGCCTTATGTTTAGGTTCTGAAAAGACACCTGAGGGTGCCACTCAATTGCAGTTCCACTGCAACCAAGTCCGCTTACTGGTGTTTCATGAAACACAACTGGCAGTTTATGATGCCTCAAAGATGAAGCTCCTCCGGCTGTGGCTTCCACACGGTATGCTTTCTGCTCCCATACGTCATGCAGCATACTCCTTTGACAGTCGACTGGTTTATACCGCCTTCAGTGATGGTAACATCGGAATATTTGATGCCAATAGCCTTAGACTGAGATGTCGTATTGCTCCATCAGCATACTTACCGGAAGCAGTCTTGAAGAGGACCGATGAATTGTATTCTCTTGTCATTGCAGGAAATCCCCATGTACAGAACCAGTTTGCTGTTGGGTTGTCTGATGGTACTGTTAAGGTCATTGAGCCATTATTTGGTGACTGGGGGTCCTCGCCACCTCCTGATGATGAAGCTTCATCAAGTGCCATCAACAACCACTCGTCCAGACAAGTACAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

1101

Amino Acids

122.9

Weight (kDa)

8.48

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 6 - 34 1.2e-08 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 1.2e-52 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 1.3e-22 TOPLESS, zinc finger domain
Beta-prop_WDR3_2nd PF25172 316 - 496 2.7e-06 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 356 - 533 8.5e-15 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 357 - 529 1.8e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 378 - 597 5e-19 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 380 - 628 4.2e-16 THOC3 beta-propeller domain
WD40_Prp19 PF24814 423 - 530 9.3e-07 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 435 - 497 4.7e-07 WDHD1 first WD40 domain
WD40_WDHD1_1st PF24817 802 - 1017 3.3e-14 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 807 - 931 9.3e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 810 - 930 1.5e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 810 - 929 1.1e-10 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 812 - 1024 5.4e-13 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 813 - 1013 1.6e-08 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 816 - 924 9.4e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 826 - 924 7.7e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 833 - 927 2.1e-06 WDR90/POC16, second beta-propeller
WD40_Prp19 PF24814 855 - 1018 1e-09 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 321, 2517
AasI GACNNNNNNGTC 2 cut(s) 290, 3063
AatII GACGTC 1 cut(s) 1918
Acc16I TGCGCA 1 cut(s) 515
Acc65I GGTACC 1 cut(s) 860
AccB1I GGYRCC 2 cut(s) 860, 2831
AccB7I CCANNNNNTGG 3 cut(s) 626, 2163, 3215
AccI GTMKAC 4 cut(s) 2063, 2644, 2722, 2995
AccIII TCCGGA 1 cut(s) 1558
AciI CCGC 8 cut(s) 748, 1085, 2366, 2435, 2525, 2686, 2866, 3009
AclWI GGATC 6 cut(s) 779, 952, 1958, 2180, 2193, 2504
AcoI YGGCCR 3 cut(s) 749, 2400, 2601
AcsI RAATTY 5 cut(s) 7, 674, 1198, 1665, 2337
AcuI CTGAAG 4 cut(s) 764, 1088, 1713, 2998
AcyI GRCGYC 3 cut(s) 1260, 1803, 1915
AfeI AGCGCT 1 cut(s) 2451
AflIII ACRYGT 2 cut(s) 838, 1325
AhdI GACNNNNNGTC 1 cut(s) 3226
AjnI CCWGG 5 cut(s) 893, 1481, 1525, 1806, 2057
Alw21I GWGCWC 2 cut(s) 441, 1112
Alw26I GTCTC 2 cut(s) 1596, 2416
AlwI GGATC 6 cut(s) 779, 952, 1958, 2180, 2193, 2504
AlwNI CAGNNNCTG 1 cut(s) 2308
Ama87I CYCGRG 2 cut(s) 2285, 2583
Aor13HI TCCGGA 1 cut(s) 1558
Aor51HI AGCGCT 1 cut(s) 2451
AoxI GGCC 8 cut(s) 69, 276, 749, 2025, 2318, 2346, 2400, 2601
ApeKI GCWGC 4 cut(s) 544, 1060, 2368, 2975
ApoI RAATTY 5 cut(s) 7, 674, 1198, 1665, 2337
ArsI GACNNNNNNTTYG 2 cut(s) 1274, 1306
AseI ATTAAT 1 cut(s) 525
Asp700I GAANNNNTTC 1 cut(s) 1669
Asp718I GGTACC 1 cut(s) 860
AspLEI GCGC 3 cut(s) 516, 2354, 2452
AsuC2I CCSGG 1 cut(s) 566
AsuHPI GGTGA 7 cut(s) 606, 971, 1025, 1754, 1823, 2386, 3230
AvaI CYCGRG 2 cut(s) 2285, 2583
AvaII GGWCC 8 cut(s) 34, 562, 629, 1502, 1774, 2390, 3111, 3228
AxyI CCTNAGG 1 cut(s) 2826
BalI TGGCCA 2 cut(s) 2402, 2603
BamHI GGATCC 1 cut(s) 2185
BanI GGYRCC 2 cut(s) 860, 2831
BanII GRGCYC 2 cut(s) 441, 2051
BbsI GAAGAC 2 cut(s) 1131, 1850
Bbv12I GWGCWC 2 cut(s) 441, 1112
BbvI GCAGC 4 cut(s) 556, 1072, 2380, 2987
BceAI ACGGC 5 cut(s) 415, 1098, 1131, 1507, 1890
BcgI CGANNNNNNTGC 4 cut(s) 814, 824, 848, 858
BciT130I CCWGG 5 cut(s) 895, 1483, 1527, 1808, 2059
BclI TGATCA 1 cut(s) 2770
BcnI CCSGG 1 cut(s) 566
BcoDI GTCTC 2 cut(s) 1596, 2416
BfaI CTAG 9 cut(s) 641, 792, 876, 995, 1506, 1613, 1794, 2328, 2736
BfmI CTRYAG 1 cut(s) 948
BfoI RGCGCY 2 cut(s) 2355, 2453
BglI GCCNNNNNGGC 1 cut(s) 2279
BglII AGATCT 1 cut(s) 1508
BisI GCNGC 6 cut(s) 545, 749, 1061, 1086, 2369, 2976
BlsI GCNGC 6 cut(s) 546, 750, 1062, 1087, 2370, 2977
BmcAI AGTACT 2 cut(s) 119, 796
Bme1390I CCNGG 6 cut(s) 566, 895, 1483, 1527, 1808, 2059
Bme18I GGWCC 8 cut(s) 34, 562, 629, 1502, 1774, 2390, 3111, 3228
BmeRI GACNNNNNGTC 1 cut(s) 3226
BmeT110I CYCGRG 2 cut(s) 2285, 2583
BmrFI CCNGG 6 cut(s) 566, 895, 1483, 1527, 1808, 2059
BmrI ACTGGG 1 cut(s) 3232
BmsI GCATC 5 cut(s) 1510, 2000, 2455, 2896, 3031
BmuI ACTGGG 1 cut(s) 3232
BoxI GACNNNNGTC 1 cut(s) 2388
BpiI GAAGAC 2 cut(s) 1131, 1850
BplI GAGNNNNNCTC 2 cut(s) 1813, 1845
BpmI CTGGAG 2 cut(s) 162, 287
BpuEI CTTGAG 2 cut(s) 104, 1855
BpuMI CCSGG 1 cut(s) 566
Bsa29I ATCGAT 1 cut(s) 199
BsaBI GATNNNNATC 1 cut(s) 969
BsaHI GRCGYC 3 cut(s) 1260, 1803, 1915
BsaJI CCNNGG 5 cut(s) 1264, 1806, 1807, 2162, 2582
BsaWI WCCGGW 3 cut(s) 816, 1558, 3091
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bse118I RCCGGY 1 cut(s) 1242
Bse21I CCTNAGG 1 cut(s) 2826
Bse3DI GCAATG 6 cut(s) 560, 986, 1015, 1501, 1544, 3135
Bse8I GATNNNNATC 1 cut(s) 969
BseAI TCCGGA 1 cut(s) 1558
BseBI CCWGG 5 cut(s) 895, 1483, 1527, 1808, 2059
BseCI ATCGAT 1 cut(s) 199
BseDI CCNNGG 5 cut(s) 1264, 1806, 1807, 2162, 2582
BseGI GGATG 4 cut(s) 825, 970, 1491, 1567
BseJI GATNNNNATC 1 cut(s) 969
BseMI GCAATG 6 cut(s) 560, 986, 1015, 1501, 1544, 3135
BseMII CTCAG 6 cut(s) 407, 1910, 2087, 2427, 2817, 3050
BseRI GAGGAG 4 cut(s) 68, 140, 2153, 2915
BseXI GCAGC 4 cut(s) 556, 1072, 2380, 2987
BsgI GTGCAG 2 cut(s) 34, 2541
Bsh1285I CGRYCG 2 cut(s) 259, 848
BshFI GGCC 8 cut(s) 71, 278, 751, 2027, 2320, 2348, 2402, 2603
BshNI GGYRCC 2 cut(s) 860, 2831
BshVI ATCGAT 1 cut(s) 199
BsiEI CGRYCG 2 cut(s) 259, 848
BsiHKAI GWGCWC 2 cut(s) 441, 1112
BsiHKCI CYCGRG 2 cut(s) 2285, 2583
BsiSI CCGG 6 cut(s) 566, 817, 1243, 1559, 2929, 3092
BslFI GGGAC 1 cut(s) 2396
BsmAI GTCTC 2 cut(s) 1596, 2416
BsmFI GGGAC 1 cut(s) 2396
BsmI GAATGC 2 cut(s) 2573, 2616
BsnI GGCC 8 cut(s) 71, 278, 751, 2027, 2320, 2348, 2402, 2603
BsoBI CYCGRG 2 cut(s) 2285, 2583
Bsp1286I GDGCHC 3 cut(s) 441, 1112, 2051
Bsp13I TCCGGA 1 cut(s) 1558
Bsp1407I TGTACA 2 cut(s) 2299, 3154
Bsp19I CCATGG 1 cut(s) 1264
BspACI CCGC 8 cut(s) 748, 1085, 2366, 2435, 2525, 2686, 2866, 3009
BspANI GGCC 8 cut(s) 71, 278, 751, 2027, 2320, 2348, 2402, 2603
BspCNI CTCAG 6 cut(s) 406, 1911, 2086, 2426, 2818, 3051
BspDI ATCGAT 1 cut(s) 199
BspEI TCCGGA 1 cut(s) 1558
BspHI TCATGA 3 cut(s) 1393, 1890, 2881
BspPI GGATC 6 cut(s) 779, 952, 1958, 2180, 2193, 2504
BspT107I GGYRCC 2 cut(s) 860, 2831
BsrDI GCAATG 6 cut(s) 560, 986, 1015, 1501, 1544, 3135
BsrFI RCCGGY 1 cut(s) 1242
BsrGI TGTACA 2 cut(s) 2299, 3154
BssAI RCCGGY 1 cut(s) 1242
BssECI CCNNGG 5 cut(s) 1264, 1806, 1807, 2162, 2582
BssNAI GTATAC 1 cut(s) 2723
BssNI GRCGYC 3 cut(s) 1260, 1803, 1915
BssT1I CCWWGG 2 cut(s) 1264, 2162
Bst1107I GTATAC 1 cut(s) 2723
Bst2UI CCWGG 5 cut(s) 895, 1483, 1527, 1808, 2059
Bst4CI ACNGT 9 cut(s) 611, 952, 1314, 2134, 2267, 2747, 2948, 2993, 3193
Bst6I CTCTTC 2 cut(s) 2424, 3101
BstACI GRCGYC 3 cut(s) 1260, 1803, 1915
BstAUI TGTACA 2 cut(s) 2299, 3154
BstC8I GCNNGC 3 cut(s) 935, 1006, 2350
BstDEI CTNAG 8 cut(s) 289, 393, 1919, 2073, 2413, 2826, 3053, 3059
BstDSI CCRYGG 1 cut(s) 1264
BstEII GGTNACC 1 cut(s) 1565
BstENI CCTNNNNNAGG 2 cut(s) 50, 2429
BstF5I GGATG 4 cut(s) 825, 970, 1491, 1567
BstH2I RGCGCY 2 cut(s) 2355, 2453
BstHHI GCGC 3 cut(s) 516, 2354, 2452
BstMAI GTCTC 2 cut(s) 1596, 2416
BstMCI CGRYCG 2 cut(s) 259, 848
BstMWI GCNNNNNNNGC 6 cut(s) 445, 550, 1988, 2279, 2929, 3080
BstNI CCWGG 5 cut(s) 895, 1483, 1527, 1808, 2059
BstNSI RCATGY 3 cut(s) 842, 937, 1329
BstPAI GACNNNNGTC 1 cut(s) 2388
BstPI GGTNACC 1 cut(s) 1565
BstSCI CCNGG 6 cut(s) 564, 893, 1481, 1525, 1806, 2057
BstSFI CTRYAG 1 cut(s) 948
BstV1I GCAGC 4 cut(s) 556, 1072, 2380, 2987
BstV2I GAAGAC 2 cut(s) 1131, 1850
BstX2I RGATCY 4 cut(s) 944, 1508, 1963, 2185
BstXI CCANNNNNNTGG 1 cut(s) 1271
BstYI RGATCY 4 cut(s) 944, 1508, 1963, 2185
BstZ17I GTATAC 1 cut(s) 2723
Bsu15I ATCGAT 1 cut(s) 199
Bsu36I CCTNAGG 1 cut(s) 2826
BsuRI GGCC 8 cut(s) 71, 278, 751, 2027, 2320, 2348, 2402, 2603
BsuTUI ATCGAT 1 cut(s) 199
BtgI CCRYGG 1 cut(s) 1264
BtsCI GGATG 4 cut(s) 825, 970, 1491, 1567
BtsI GCAGTG 3 cut(s) 1935, 1977, 2851
BtsIMutI CAGTG 9 cut(s) 189, 206, 1419, 1935, 1977, 2026, 2362, 2851, 3022
Cac8I GCNNGC 3 cut(s) 935, 1006, 2350
CaiI CAGNNNCTG 1 cut(s) 2308
CciI TCATGA 3 cut(s) 1393, 1890, 2881
CfoI GCGC 3 cut(s) 516, 2354, 2452
Cfr10I RCCGGY 1 cut(s) 1242
ClaI ATCGAT 1 cut(s) 199
CseI GACGC 3 cut(s) 926, 1268, 1811
CspCI CAANNNNNGTGG 2 cut(s) 903, 938
DdeI CTNAG 8 cut(s) 289, 393, 1919, 2073, 2413, 2826, 3053, 3059
DrdI GACNNNNNNGTC 2 cut(s) 290, 3063
DriI GACNNNNNGTC 1 cut(s) 3226
DseDI GACNNNNNNGTC 2 cut(s) 290, 3063
EaeI YGGCCR 3 cut(s) 749, 2400, 2601
Eam1104I CTCTTC 2 cut(s) 2424, 3101
Eam1105I GACNNNNNGTC 1 cut(s) 3226
EarI CTCTTC 2 cut(s) 2424, 3101
Ecl136II GAGCTC 1 cut(s) 439
Eco130I CCWWGG 2 cut(s) 1264, 2162
Eco24I GRGCYC 2 cut(s) 441, 2051
Eco47I GGWCC 8 cut(s) 34, 562, 629, 1502, 1774, 2390, 3111, 3228
Eco47III AGCGCT 1 cut(s) 2451
Eco53kI GAGCTC 1 cut(s) 439
Eco57I CTGAAG 4 cut(s) 764, 1088, 1713, 2998
Eco81I CCTNAGG 1 cut(s) 2826
Eco88I CYCGRG 2 cut(s) 2285, 2583
Eco91I GGTNACC 1 cut(s) 1565
EcoICRI GAGCTC 1 cut(s) 439
EcoNI CCTNNNNNAGG 2 cut(s) 50, 2429
EcoO109I RGGNCCY 3 cut(s) 562, 1502, 3228
EcoO65I GGTNACC 1 cut(s) 1565
EcoRII CCWGG 5 cut(s) 893, 1481, 1525, 1806, 2057
EcoT14I CCWWGG 2 cut(s) 1264, 2162
EcoT22I ATGCAT 2 cut(s) 78, 988
EcoT38I GRGCYC 2 cut(s) 441, 2051
ErhI CCWWGG 2 cut(s) 1264, 2162
FalI AAGNNNNNCTT 4 cut(s) 1039, 1071, 1842, 1874
FaqI GGGAC 1 cut(s) 2396
FbaI TGATCA 1 cut(s) 2770
FblI GTMKAC 4 cut(s) 2063, 2644, 2722, 2995
Fnu4HI GCNGC 6 cut(s) 545, 749, 1061, 1086, 2369, 2976
FokI GGATG 4 cut(s) 832, 957, 1498, 1574
FriOI GRGCYC 2 cut(s) 441, 2051
Fsp4HI GCNGC 6 cut(s) 545, 749, 1061, 1086, 2369, 2976
FspBI CTAG 9 cut(s) 641, 792, 876, 995, 1506, 1613, 1794, 2328, 2736
FspI TGCGCA 1 cut(s) 515
GlaI GCGC 3 cut(s) 515, 2353, 2451
GluI GCNGC 6 cut(s) 545, 749, 1061, 1086, 2369, 2976
GsuI CTGGAG 2 cut(s) 162, 287
HaeII RGCGCY 2 cut(s) 2355, 2453
HaeIII GGCC 8 cut(s) 71, 278, 751, 2027, 2320, 2348, 2402, 2603
HapII CCGG 6 cut(s) 566, 817, 1243, 1559, 2929, 3092
HgaI GACGC 3 cut(s) 926, 1268, 1811
HhaI GCGC 3 cut(s) 516, 2354, 2452
Hin1I GRCGYC 3 cut(s) 1260, 1803, 1915
Hin6I GCGC 3 cut(s) 514, 2352, 2450
HinP1I GCGC 3 cut(s) 514, 2352, 2450
HincII GTYRAC 4 cut(s) 1258, 2172, 2422, 2996
HindII GTYRAC 4 cut(s) 1258, 2172, 2422, 2996
HindIII AAGCTT 2 cut(s) 479, 3252
HpaII CCGG 6 cut(s) 566, 817, 1243, 1559, 2929, 3092
HphI GGTGA 7 cut(s) 606, 971, 1025, 1754, 1823, 2386, 3230
Hpy99I CGWCG 1 cut(s) 2747
HpyCH4III ACNGT 9 cut(s) 611, 952, 1314, 2134, 2267, 2747, 2948, 2993, 3193
HpyCH4IV ACGT 4 cut(s) 1090, 1437, 1915, 2968
HpyF10VI GCNNNNNNNGC 6 cut(s) 445, 550, 1988, 2279, 2929, 3080
HpyF3I CTNAG 8 cut(s) 289, 393, 1919, 2073, 2413, 2826, 3053, 3059
HpySE526I ACGT 4 cut(s) 1090, 1437, 1915, 2968
Hsp92I GRCGYC 3 cut(s) 1260, 1803, 1915
HspAI GCGC 3 cut(s) 514, 2352, 2450
Kpn2I TCCGGA 1 cut(s) 1558
KpnI GGTACC 1 cut(s) 864
Ksp22I TGATCA 1 cut(s) 2770
Lsp1109I GCAGC 4 cut(s) 556, 1072, 2380, 2987
LweI GCATC 5 cut(s) 1510, 2000, 2455, 2896, 3031
MaeI CTAG 9 cut(s) 641, 792, 876, 995, 1506, 1613, 1794, 2328, 2736
MaeII ACGT 4 cut(s) 1090, 1437, 1915, 2968
MfeI CAATTG 2 cut(s) 669, 2840
MflI RGATCY 4 cut(s) 944, 1508, 1963, 2185
MhlI GDGCHC 3 cut(s) 441, 1112, 2051
MlsI TGGCCA 2 cut(s) 2402, 2603
MluNI TGGCCA 2 cut(s) 2402, 2603
MlyI GAGTC 5 cut(s) 541, 1168, 1831, 1941, 2241
Mox20I TGGCCA 2 cut(s) 2402, 2603
Mph1103I ATGCAT 2 cut(s) 78, 988
MroI TCCGGA 1 cut(s) 1558
MroXI GAANNNNTTC 1 cut(s) 1669
MscI TGGCCA 2 cut(s) 2402, 2603
MseI TTAA 6 cut(s) 356, 525, 537, 1644, 2649, 3195
MslI CAYNNNNRTG 5 cut(s) 959, 1212, 1269, 1782, 2609
Msp20I TGGCCA 2 cut(s) 2402, 2603
MspA1I CMGCKG 1 cut(s) 2371
MspI CCGG 6 cut(s) 566, 817, 1243, 1559, 2929, 3092
MspR9I CCNGG 6 cut(s) 566, 895, 1483, 1527, 1808, 2059
MunI CAATTG 2 cut(s) 669, 2840
Mva1269I GAATGC 2 cut(s) 2573, 2616
MvaI CCWGG 5 cut(s) 895, 1483, 1527, 1808, 2059
MwoI GCNNNNNNNGC 6 cut(s) 445, 550, 1988, 2279, 2929, 3080
NciI CCSGG 1 cut(s) 566
NcoI CCATGG 1 cut(s) 1264
NmeAIII GCCGAG 2 cut(s) 1021, 1845
NmuCI GTSAC 5 cut(s) 952, 1867, 1933, 1949, 3218
NsbI TGCGCA 1 cut(s) 515
NsiI ATGCAT 2 cut(s) 78, 988
NspI RCATGY 3 cut(s) 842, 937, 1329
PaeI GCATGC 1 cut(s) 937
PagI TCATGA 3 cut(s) 1393, 1890, 2881
PasI CCCWGGG 1 cut(s) 1807
PciI ACATGT 2 cut(s) 838, 1325
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PctI GAATGC 2 cut(s) 2573, 2616
PdmI GAANNNNTTC 1 cut(s) 1669
PfeI GAWTC 7 cut(s) 92, 574, 1204, 1839, 1887, 1908, 1973
PflMI CCANNNNNTGG 3 cut(s) 626, 2163, 3215
PkrI GCNGC 6 cut(s) 546, 750, 1062, 1087, 2370, 2977
Ple19I CGATCG 2 cut(s) 259, 848
PleI GAGTC 5 cut(s) 540, 1167, 1830, 1940, 2241
PpsI GAGTC 5 cut(s) 540, 1167, 1830, 1940, 2241
PpuMI RGGWCCY 3 cut(s) 562, 1502, 3228
PscI ACATGT 2 cut(s) 838, 1325
PshAI GACNNNNGTC 1 cut(s) 2388
PshBI ATTAAT 1 cut(s) 525
PsiI TTATAA 2 cut(s) 321, 2517
Psp124BI GAGCTC 1 cut(s) 441
Psp5II RGGWCCY 3 cut(s) 562, 1502, 3228
Psp6I CCWGG 5 cut(s) 893, 1481, 1525, 1806, 2057
PspEI GGTNACC 1 cut(s) 1565
PspGI CCWGG 5 cut(s) 893, 1481, 1525, 1806, 2057
PspPPI RGGWCCY 3 cut(s) 562, 1502, 3228
PstNI CAGNNNCTG 1 cut(s) 2308
PsuI RGATCY 4 cut(s) 944, 1508, 1963, 2185
PvuI CGATCG 2 cut(s) 259, 848
PvuII CAGCTG 1 cut(s) 2371
RseI CAYNNNNRTG 5 cut(s) 959, 1212, 1269, 1782, 2609
SacI GAGCTC 1 cut(s) 441
SalI GTCGAC 1 cut(s) 2994
SaqAI TTAA 6 cut(s) 356, 525, 537, 1644, 2649, 3195
SatI GCNGC 6 cut(s) 545, 749, 1061, 1086, 2369, 2976
ScaI AGTACT 2 cut(s) 119, 796
SchI GAGTC 5 cut(s) 541, 1168, 1831, 1941, 2241
ScrFI CCNGG 6 cut(s) 566, 895, 1483, 1527, 1808, 2059
SduI GDGCHC 3 cut(s) 441, 1112, 2051
SfaNI GCATC 5 cut(s) 1510, 2000, 2455, 2896, 3031
SfcI CTRYAG 1 cut(s) 948
SinI GGWCC 8 cut(s) 34, 562, 629, 1502, 1774, 2390, 3111, 3228
SmiMI CAYNNNNRTG 5 cut(s) 959, 1212, 1269, 1782, 2609
SmlI CTYRAG 2 cut(s) 83, 1834
SmoI CTYRAG 2 cut(s) 83, 1834
SphI GCATGC 1 cut(s) 937
SsiI CCGC 8 cut(s) 748, 1085, 2366, 2435, 2525, 2686, 2866, 3009
SspI AATATT 1 cut(s) 3036
SspMI CTAG 9 cut(s) 641, 792, 876, 995, 1506, 1613, 1794, 2328, 2736
SstI GAGCTC 1 cut(s) 441
StyD4I CCNGG 6 cut(s) 564, 893, 1481, 1525, 1806, 2057
StyI CCWWGG 2 cut(s) 1264, 2162
TaaI ACNGT 9 cut(s) 611, 952, 1314, 2134, 2267, 2747, 2948, 2993, 3193
TaiI ACGT 4 cut(s) 1093, 1440, 1918, 2971
TaqII GACCGA 1 cut(s) 3128
TatI WGTACW 6 cut(s) 117, 794, 1443, 2299, 3154, 3292
TauI GCSGC 2 cut(s) 751, 1088
TfiI GAWTC 7 cut(s) 92, 574, 1204, 1839, 1887, 1908, 1973
Tru1I TTAA 6 cut(s) 356, 525, 537, 1644, 2649, 3195
Tru9I TTAA 6 cut(s) 356, 525, 537, 1644, 2649, 3195
TscAI CASTG 9 cut(s) 196, 213, 1419, 1942, 1984, 2026, 2362, 2858, 3022
TseFI GTSAC 5 cut(s) 952, 1867, 1933, 1949, 3218
TseI GCWGC 4 cut(s) 544, 1060, 2368, 2975
Tsp45I GTSAC 5 cut(s) 952, 1867, 1933, 1949, 3218
TspGWI ACGGA 2 cut(s) 1814, 2140
TspRI CASTG 9 cut(s) 196, 213, 1419, 1942, 1984, 2026, 2362, 2858, 3022
Van91I CCANNNNNTGG 3 cut(s) 626, 2163, 3215
VpaK11BI GGWCC 8 cut(s) 34, 562, 629, 1502, 1774, 2390, 3111, 3228
VspI ATTAAT 1 cut(s) 525
XagI CCTNNNNNAGG 2 cut(s) 50, 2429
XapI RAATTY 5 cut(s) 7, 674, 1198, 1665, 2337
XbaI TCTAGA 1 cut(s) 875
XceI RCATGY 3 cut(s) 842, 937, 1329
XcmI CCANNNNNNNNNTGG 2 cut(s) 1071, 3170
XmiI GTMKAC 4 cut(s) 2063, 2644, 2722, 2995
XmnI GAANNNNTTC 1 cut(s) 1669
XspI CTAG 9 cut(s) 641, 792, 876, 995, 1506, 1613, 1794, 2328, 2736
ZraI GACGTC 1 cut(s) 1916
ZrmI AGTACT 2 cut(s) 119, 796
Zsp2I ATGCAT 2 cut(s) 78, 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.