Rh3CG075700

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0005953
Physical Location & Seq
Forward (+)
5971623 .. 5980229
8607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG075700.1

Sequence Viewer

Length: 3288 bp
ATGTCAAATTTGTGCAGGGAATTGGTGTTACTGGTCCTCCAATTCCTTGACGAGGAGAAGTACAGAGGGGCCATGCATAAACTTGAGCAAGAATCAGGGTTCTTTTTCAACATGAAGTACTTTGAGGAGAAGATTTGGAGTGGAGAATGGGAAGAAGTTGAAAAGTATTTATCAGGATTTACAAAACTCAATGACAATCGATTTTCACTGAAAATAATGTTTGAAATATCAAAGCAGAAATATCTGGAAGCCCTCGATCGGAACGAGAAGGTTGGGGCCATTGACATCTTAGTCAATGATCTCCAGAAGTTTGCTCCTTATAATCAAGAACTTTACAAGGAGATCACACAGCTTTTAACCCTCCGAAACTTTCGGGAAAATGCACATCTCACTCAGTACGGCGACACCAGATCAGCTCGTCATAGAATGGTGGTAGAGCTCAAAAAGCTCATAAAAGCAAACCCTCTATTGAAGGATAAGCTTTATTTTCCTTCTGTGAGGTCAGCCAGACTGCGGACTCTCATTAATCAGAGTCTTAACTGGCAGCACCAGCAATGCAAGGACCCGGTTCTGAATCCAGAAATAAAAACTCTGTTCATAGATCATTACTGTCCACCACCTCTAACTGGTCCTCTTGCTCTAGCAAATGTCAATCAGCCATTTCCTGCAATTGAATTTCCTGCTCCTTTTCCCTCAATGGAAGGAGCTATTGTTCCGTATCGGGCCCCTGGAGAAGTTCCAAGCCTAAAGCAGCCAAGAACAGCTCTGGAAACTTCTGGTGGGGTTGATCCTTCTAGTTTTACTGCTATTGACCGGATGGCGAAAGAACTGCAACATATTCGATCGAACTTGGAGGGTACTTTTCAAACTTCTAGACTGCTTCAAACTTCCTGGTTGGTGGAGAACCTACCGAGAAATGTGGCTTATAGCATGCGTCAAGGATCTACAGTCACAAGCATGGATTTTCATCCTTATCACCATGCATTGCTCCTAGTTGGTGCTAGCAATGGTGAAATTAGTCTTTGGGAAGTTGGAACTCGGCAGAGGGTGGCTTCGCAGCCATTCAGAATATGGAATACGGCAGCACGTTCATCAGAACTTCAGAGCACACTTGCCGATGAAGACACATCTATATCTGTGAGCCGTGTTACATGGAGTCCAGATGGGAACTTTTTTGGAGTTGCATTTACTAAAAATTTGATTCACTTGTATGCTTTTTCTGGAATTAGTGATCTATGCCGGTATTTGGAGGTTGACGCCCATGATGGTGGTGTGAATGACTTGGCTTTTGATCATCGAAACAACGAACTGTGTGTTATCACATGTGGAGATGACAAGCTAATAAAGGTATGGGATATATCAGGACGAAACTTATATAACTTTGATGGTCATGAAGCACCGGTCTATTCAGTGTGTCCTTATCATAACAAGGATGTTCAGTACATATATTCAACTTCTACTGATGGGAAGATAAAGACCTGGATGTACGACAATGGGGGTCCTAGATCTGGCTGTGATGCTCCTGGCAACTGGTGTACTACATTGCTTTTCAGTCCGGATGGTAACCGAATGTTCTCTTGTGGAACAAATAAAGAGGGAGACACTTTTCTAGTTCAATGGAGCGAAAATGAAGGACTAATTAAAGAAGGAGCAAAAAAGAGAATTTATTCTGGGTTTGGTAAGAAATCTGAAGGGGTTGTGCATTTTGACATAACGAAAAAGCATTTTTTGGCTGTGGGGGAAGATAGCCAGATCAAGTATTGGGATATGGACCATGATGATGTTCTGACTAGCACAGACGCCCAGGGTGAACTGCCGAGTCGTCCTCGCTTGAGATTCAACAAGGAAGGTAGTCTTCTTGCTGTCACAACGGCACATGAGGGATTCATGATACTTGCAAATGGAATCGGACGCCTGAGAGTTCATGGAGTCGCTGCAACTTATTGTGACAGGTCGAAAGATCCTACTGAATCCACTGCAAACAAGGCATCTGGCTCATCTTCGGTTGCAAATGTCAGTGAGGCCAATAGGAAATCTGACAGGGGCTCTCCTGCTAGGTCTACAACATCTCAGAATGGAGCTAATACCAAGAGTGGAAGTTCCAAGAAACGGAAAACTGTGGAGGAGGTAATCAATAAATCCAAACCTTGGCAGTTGACTGACATTCGGGATCCTGTAAGGTGTCGTTTAGGTGTTATGCCTGAAACAGATAGTTCCTGCAAGGTTGTTCGACTCCTATATACAAGTAACGGTACTGCCATCTTGGCACTTGGGTCAAATGGTGTACAGAGGCTGTGGAGGTGGCCCCGAACTAGAGGTCGAATTTCAAAGGCCAGCGCCAGTGTTGTTCCGCAGCTGGTGAAAGGGGACAATGGTCCTGTTATGGCCAATGATGTCTCAGGTGTCAACCTTGAAGAGGTGGTTCCCTGCATAGCACTCTCAAAGAATGACGCGTATGTAATGTCAGCTTATGGTGGAAGGATCAGATTATTCAACATTTATAATTTGGCGGTAATGATAACTTTCGGGGAACTTCCCCCTGCACCAACTGAAATTGCATTCAATCCTCGGGATAATAATATCATGGCCATTGGAATGCAAGATTGCACCATACAGATTTTCTTTTGTAGACTTAATGAGGTAAGAGCAATCTTGAAGTTTCACAAGAAGTGGATAACTGGTTTAGTTTTCTCGTTCTTTCTTGGTATACTCATTTCAGCTAGTGTCGATGGTCAAATATGTGTGTGGAGCACTGATCAATGGGAGAAGAAACAATCTGTTGCCTTATGTTTAGGTTCTGAAAAGACACCTGAGGGTGCCATTCAATTGCAGTTCCACTGCAACCAAGTCCGCTTACTGGTGTTTCATGAAACACAACTGGCAGTTTATGATGCCTCAAAGATGAAGCTCCTCCGGCAGTGGCTTCCACACGGTATGCTTTCTGCTCCCATACGTCATGCAGCATACTCCTTTGACAGTCGACTGGTTTATACCGCCTTCAGTGATGGTAACATCGGAGTATTTGATGCCAATAGCCTAAGACTGAGATGTCGTATTGCTCCATCAGCATACTTACCAGAAGCAGTCTTGAAGAGGACCGATGAATTGTATCCTCTCGTCATTGCAGGAAATCCCCATGTACAAAACCAGTTTGCTGTTGGGTTGTCTGATGGTACTGTTAAGGTCATTGAGCCATTATTTGGTGACTGGGGGTCCTCCCCACCTCCTGATGATGAAGCTTCATCAAGTGCCACCAACAACCACTCGTCCAGACAAGTACAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

1095

Amino Acids

122.59

Weight (kDa)

8.09

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 6 - 34 1.3e-07 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 3.8e-53 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 8.1e-22 TOPLESS, zinc finger domain
Beta-prop_WDR3_2nd PF25172 315 - 531 4.9e-07 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 317 - 459 5.6e-06 RIG second beta-propeller
Beta-prop_WDR3_1st PF25173 355 - 532 4e-16 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 356 - 530 1.4e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 378 - 596 8.5e-20 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 379 - 627 9.7e-17 THOC3 beta-propeller domain
WD40_Prp19 PF24814 420 - 531 9e-08 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 427 - 498 1.3e-07 WDHD1 first WD40 domain
EIF3I PF24805 439 - 601 6.2e-06 EIF3I
WD40_WDHD1_1st PF24817 797 - 1012 1.7e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 802 - 926 8.1e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 806 - 921 8.3e-09 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 808 - 1018 6.7e-12 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 809 - 1008 2.9e-06 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 810 - 1019 3e-08 THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 827 - 922 8.8e-06 WDR90/POC16, second beta-propeller
WD40_Prp19 PF24814 850 - 1013 2e-07 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 321, 2502
AasI GACNNNNNNGTC 2 cut(s) 290, 3048
AccB1I GGYRCC 1 cut(s) 2816
AccB7I CCANNNNNTGG 2 cut(s) 2148, 3200
AccI GTMKAC 4 cut(s) 2060, 2629, 2707, 2980
AccII CGCG 1 cut(s) 2453
AccIII TCCGGA 1 cut(s) 1555
AciI CCGC 5 cut(s) 514, 2351, 2510, 2851, 2994
AclWI GGATC 6 cut(s) 782, 949, 1955, 2165, 2178, 2489
AcoI YGGCCR 2 cut(s) 2385, 2586
AcsI RAATTY 5 cut(s) 7, 674, 1195, 1662, 2322
AcuI CTGAAG 3 cut(s) 1085, 1710, 2983
AcyI GRCGYC 3 cut(s) 1257, 1800, 1912
AflIII ACRYGT 2 cut(s) 1322, 2451
AgeI ACCGGT 1 cut(s) 1399
AhdI GACNNNNNGTC 1 cut(s) 3211
AjnI CCWGG 5 cut(s) 727, 890, 1478, 1522, 1803
AloI GAACNNNNNNTCC 2 cut(s) 696, 728
Alw21I GWGCWC 3 cut(s) 441, 1109, 2753
Alw26I GTCTC 2 cut(s) 1593, 2401
AlwI GGATC 6 cut(s) 782, 949, 1955, 2165, 2178, 2489
AlwNI CAGNNNCTG 1 cut(s) 2293
Ama87I CYCGRG 1 cut(s) 2568
Aor13HI TCCGGA 1 cut(s) 1555
AoxI GGCC 8 cut(s) 69, 276, 723, 2022, 2303, 2331, 2385, 2586
ApaI GGGCCC 1 cut(s) 727
ApeKI GCWGC 7 cut(s) 544, 751, 1057, 1082, 1934, 2353, 2960
ApoI RAATTY 5 cut(s) 7, 674, 1195, 1662, 2322
ArsI GACNNNNNNTTYG 4 cut(s) 1271, 1303, 2302, 2334
AseI ATTAAT 1 cut(s) 525
AsiGI ACCGGT 1 cut(s) 1399
Asp700I GAANNNNTTC 1 cut(s) 1666
AspLEI GCGC 1 cut(s) 2339
AsuC2I CCSGG 1 cut(s) 566
AsuHPI GGTGA 5 cut(s) 968, 1022, 1820, 2371, 3215
AsuNHI GCTAGC 1 cut(s) 1001
AvaI CYCGRG 1 cut(s) 2568
AvaII GGWCC 8 cut(s) 34, 562, 629, 1499, 1771, 2375, 3096, 3213
AxyI CCTNAGG 1 cut(s) 2811
BaeGI GKGCMC 1 cut(s) 727
BalI TGGCCA 2 cut(s) 2387, 2588
BamHI GGATCC 1 cut(s) 2170
BanI GGYRCC 1 cut(s) 2816
BanII GRGCYC 3 cut(s) 441, 727, 2048
BbsI GAAGAC 2 cut(s) 1128, 1847
Bbv12I GWGCWC 3 cut(s) 441, 1109, 2753
BbvI GCAGC 7 cut(s) 556, 763, 1069, 1094, 1921, 2365, 2972
BceAI ACGGC 4 cut(s) 415, 1095, 1128, 1887
BcgI CGANNNNNNTGC 4 cut(s) 811, 821, 845, 855
BciT130I CCWGG 5 cut(s) 729, 892, 1480, 1524, 1805
BciVI GTATCC 1 cut(s) 3120
BclI TGATCA 2 cut(s) 1291, 2755
BcnI CCSGG 1 cut(s) 566
BcoDI GTCTC 2 cut(s) 1593, 2401
BfmI CTRYAG 1 cut(s) 945
BfoI RGCGCY 1 cut(s) 2340
BfuI GTATCC 1 cut(s) 3120
BglI GCCNNNNNGGC 1 cut(s) 2264
BglII AGATCT 1 cut(s) 1505
BisI GCNGC 7 cut(s) 545, 752, 1058, 1083, 1935, 2354, 2961
BlsI GCNGC 7 cut(s) 546, 753, 1059, 1084, 1936, 2355, 2962
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 6 cut(s) 566, 729, 892, 1480, 1524, 1805
Bme18I GGWCC 8 cut(s) 34, 562, 629, 1499, 1771, 2375, 3096, 3213
BmeRI GACNNNNNGTC 1 cut(s) 3211
BmeT110I CYCGRG 1 cut(s) 2568
BmrFI CCNGG 6 cut(s) 566, 729, 892, 1480, 1524, 1805
BmrI ACTGGG 1 cut(s) 3217
BmsI GCATC 4 cut(s) 1507, 1997, 2881, 3016
BmtI GCTAGC 1 cut(s) 1005
BmuI ACTGGG 1 cut(s) 3217
BoxI GACNNNNGTC 1 cut(s) 2373
BpiI GAAGAC 2 cut(s) 1128, 1847
BplI GAGNNNNNCTC 2 cut(s) 1810, 1842
BpmI CTGGAG 2 cut(s) 287, 750
BpuEI CTTGAG 2 cut(s) 104, 1852
BpuMI CCSGG 1 cut(s) 566
Bsa29I ATCGAT 1 cut(s) 199
BsaBI GATNNNNATC 1 cut(s) 966
BsaHI GRCGYC 3 cut(s) 1257, 1800, 1912
BsaJI CCNNGG 5 cut(s) 727, 1803, 1804, 2147, 2567
BsaWI WCCGGW 3 cut(s) 813, 1399, 1555
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 696, 726
Bse118I RCCGGY 2 cut(s) 1239, 1399
Bse21I CCTNAGG 1 cut(s) 2811
Bse3DI GCAATG 5 cut(s) 560, 983, 1012, 1541, 3120
Bse8I GATNNNNATC 1 cut(s) 966
BseAI TCCGGA 1 cut(s) 1555
BseBI CCWGG 5 cut(s) 729, 892, 1480, 1524, 1805
BseCI ATCGAT 1 cut(s) 199
BseDI CCNNGG 5 cut(s) 727, 1803, 1804, 2147, 2567
BseGI GGATG 5 cut(s) 822, 967, 1438, 1488, 1564
BseJI GATNNNNATC 1 cut(s) 966
BseMI GCAATG 5 cut(s) 560, 983, 1012, 1541, 3120
BseMII CTCAG 6 cut(s) 407, 1907, 2084, 2412, 2802, 3035
BseRI GAGGAG 4 cut(s) 68, 140, 2138, 2900
BseSI GKGCMC 1 cut(s) 727
BseXI GCAGC 7 cut(s) 556, 763, 1069, 1094, 1921, 2365, 2972
BsgI GTGCAG 2 cut(s) 34, 2526
Bsh1236I CGCG 1 cut(s) 2453
Bsh1285I CGRYCG 2 cut(s) 259, 845
BshFI GGCC 8 cut(s) 71, 278, 725, 2024, 2305, 2333, 2387, 2588
BshNI GGYRCC 1 cut(s) 2816
BshTI ACCGGT 1 cut(s) 1399
BshVI ATCGAT 1 cut(s) 199
BsiEI CGRYCG 2 cut(s) 259, 845
BsiHKAI GWGCWC 3 cut(s) 441, 1109, 2753
BsiHKCI CYCGRG 1 cut(s) 2568
BsiSI CCGG 6 cut(s) 566, 814, 1240, 1400, 1556, 2914
BslFI GGGAC 1 cut(s) 2381
BsmAI GTCTC 2 cut(s) 1593, 2401
BsmFI GGGAC 1 cut(s) 2381
BsmI GAATGC 2 cut(s) 2558, 2601
BsnI GGCC 8 cut(s) 71, 278, 725, 2024, 2305, 2333, 2387, 2588
BsoBI CYCGRG 1 cut(s) 2568
Bsp120I GGGCCC 1 cut(s) 723
Bsp1286I GDGCHC 5 cut(s) 441, 727, 1109, 2048, 2753
Bsp13I TCCGGA 1 cut(s) 1555
Bsp1407I TGTACA 2 cut(s) 2284, 3139
BspACI CCGC 5 cut(s) 514, 2351, 2510, 2851, 2994
BspANI GGCC 8 cut(s) 71, 278, 725, 2024, 2305, 2333, 2387, 2588
BspCNI CTCAG 6 cut(s) 406, 1908, 2083, 2411, 2803, 3036
BspDI ATCGAT 1 cut(s) 199
BspEI TCCGGA 1 cut(s) 1555
BspFNI CGCG 1 cut(s) 2453
BspHI TCATGA 3 cut(s) 1390, 1887, 2866
BspOI GCTAGC 1 cut(s) 1005
BspPI GGATC 6 cut(s) 782, 949, 1955, 2165, 2178, 2489
BspT107I GGYRCC 1 cut(s) 2816
BsrDI GCAATG 5 cut(s) 560, 983, 1012, 1541, 3120
BsrFI RCCGGY 2 cut(s) 1239, 1399
BsrGI TGTACA 2 cut(s) 2284, 3139
BssAI RCCGGY 2 cut(s) 1239, 1399
BssECI CCNNGG 5 cut(s) 727, 1803, 1804, 2147, 2567
BssNAI GTATAC 1 cut(s) 2708
BssNI GRCGYC 3 cut(s) 1257, 1800, 1912
BssT1I CCWWGG 1 cut(s) 2147
Bst1107I GTATAC 1 cut(s) 2708
Bst2UI CCWGG 5 cut(s) 729, 892, 1480, 1524, 1805
Bst4CI ACNGT 8 cut(s) 611, 949, 1311, 2119, 2252, 2933, 2978, 3178
Bst6I CTCTTC 2 cut(s) 2409, 3086
BstACI GRCGYC 3 cut(s) 1257, 1800, 1912
BstAUI TGTACA 2 cut(s) 2284, 3139
BstC8I GCNNGC 3 cut(s) 932, 1003, 2335
BstDEI CTNAG 8 cut(s) 289, 393, 1916, 2070, 2398, 2811, 3038, 3044
BstEII GGTNACC 1 cut(s) 1562
BstENI CCTNNNNNAGG 2 cut(s) 50, 2414
BstF5I GGATG 5 cut(s) 822, 967, 1438, 1488, 1564
BstFNI CGCG 1 cut(s) 2453
BstH2I RGCGCY 1 cut(s) 2340
BstHHI GCGC 1 cut(s) 2339
BstMAI GTCTC 2 cut(s) 1593, 2401
BstMCI CGRYCG 2 cut(s) 259, 845
BstMWI GCNNNNNNNGC 6 cut(s) 445, 550, 1985, 2264, 2914, 3065
BstNI CCWGG 5 cut(s) 729, 892, 1480, 1524, 1805
BstNSI RCATGY 2 cut(s) 934, 1326
BstPAI GACNNNNGTC 1 cut(s) 2373
BstPI GGTNACC 1 cut(s) 1562
BstSCI CCNGG 6 cut(s) 564, 727, 890, 1478, 1522, 1803
BstSFI CTRYAG 1 cut(s) 945
BstSLI GKGCMC 1 cut(s) 727
BstUI CGCG 1 cut(s) 2453
BstV1I GCAGC 7 cut(s) 556, 763, 1069, 1094, 1921, 2365, 2972
BstV2I GAAGAC 2 cut(s) 1128, 1847
BstX2I RGATCY 4 cut(s) 941, 1505, 1960, 2170
BstXI CCANNNNNNTGG 1 cut(s) 1268
BstYI RGATCY 4 cut(s) 941, 1505, 1960, 2170
BstZ17I GTATAC 1 cut(s) 2708
Bsu15I ATCGAT 1 cut(s) 199
Bsu36I CCTNAGG 1 cut(s) 2811
BsuI GTATCC 1 cut(s) 3120
BsuRI GGCC 8 cut(s) 71, 278, 725, 2024, 2305, 2333, 2387, 2588
BsuTUI ATCGAT 1 cut(s) 199
BtsCI GGATG 5 cut(s) 822, 967, 1438, 1488, 1564
BtsI GCAGTG 3 cut(s) 1974, 2836, 2924
BtsIMutI CAGTG 9 cut(s) 206, 1416, 1974, 2023, 2347, 2751, 2836, 2924, 3007
Cac8I GCNNGC 3 cut(s) 932, 1003, 2335
CaiI CAGNNNCTG 1 cut(s) 2293
CciI TCATGA 3 cut(s) 1390, 1887, 2866
CfoI GCGC 1 cut(s) 2339
Cfr10I RCCGGY 2 cut(s) 1239, 1399
ClaI ATCGAT 1 cut(s) 199
CseI GACGC 5 cut(s) 923, 1265, 1808, 1920, 2459
CspAI ACCGGT 1 cut(s) 1399
DdeI CTNAG 8 cut(s) 289, 393, 1916, 2070, 2398, 2811, 3038, 3044
DrdI GACNNNNNNGTC 2 cut(s) 290, 3048
DriI GACNNNNNGTC 1 cut(s) 3211
DseDI GACNNNNNNGTC 2 cut(s) 290, 3048
EaeI YGGCCR 2 cut(s) 2385, 2586
Eam1104I CTCTTC 2 cut(s) 2409, 3086
Eam1105I GACNNNNNGTC 1 cut(s) 3211
EarI CTCTTC 2 cut(s) 2409, 3086
Ecl136II GAGCTC 1 cut(s) 439
Eco130I CCWWGG 1 cut(s) 2147
Eco24I GRGCYC 3 cut(s) 441, 727, 2048
Eco47I GGWCC 8 cut(s) 34, 562, 629, 1499, 1771, 2375, 3096, 3213
Eco53kI GAGCTC 1 cut(s) 439
Eco57I CTGAAG 3 cut(s) 1085, 1710, 2983
Eco81I CCTNAGG 1 cut(s) 2811
Eco88I CYCGRG 1 cut(s) 2568
Eco91I GGTNACC 1 cut(s) 1562
EcoICRI GAGCTC 1 cut(s) 439
EcoNI CCTNNNNNAGG 2 cut(s) 50, 2414
EcoO109I RGGNCCY 4 cut(s) 562, 724, 1499, 3213
EcoO65I GGTNACC 1 cut(s) 1562
EcoRII CCWGG 5 cut(s) 727, 890, 1478, 1522, 1803
EcoT14I CCWWGG 1 cut(s) 2147
EcoT22I ATGCAT 2 cut(s) 78, 985
EcoT38I GRGCYC 3 cut(s) 441, 727, 2048
ErhI CCWWGG 1 cut(s) 2147
FalI AAGNNNNNCTT 2 cut(s) 1839, 1871
FaqI GGGAC 1 cut(s) 2381
FbaI TGATCA 2 cut(s) 1291, 2755
FblI GTMKAC 4 cut(s) 2060, 2629, 2707, 2980
Fnu4HI GCNGC 7 cut(s) 545, 752, 1058, 1083, 1935, 2354, 2961
FokI GGATG 5 cut(s) 829, 954, 1445, 1495, 1571
FriOI GRGCYC 3 cut(s) 441, 727, 2048
Fsp4HI GCNGC 7 cut(s) 545, 752, 1058, 1083, 1935, 2354, 2961
GlaI GCGC 1 cut(s) 2338
GluI GCNGC 7 cut(s) 545, 752, 1058, 1083, 1935, 2354, 2961
GsuI CTGGAG 2 cut(s) 287, 750
HaeII RGCGCY 1 cut(s) 2340
HaeIII GGCC 8 cut(s) 71, 278, 725, 2024, 2305, 2333, 2387, 2588
HapII CCGG 6 cut(s) 566, 814, 1240, 1400, 1556, 2914
HgaI GACGC 5 cut(s) 923, 1265, 1808, 1920, 2459
HhaI GCGC 1 cut(s) 2339
Hin1I GRCGYC 3 cut(s) 1257, 1800, 1912
Hin6I GCGC 1 cut(s) 2337
HinP1I GCGC 1 cut(s) 2337
HincII GTYRAC 4 cut(s) 1255, 2157, 2407, 2981
HindII GTYRAC 4 cut(s) 1255, 2157, 2407, 2981
HindIII AAGCTT 2 cut(s) 479, 3237
HpaII CCGG 6 cut(s) 566, 814, 1240, 1400, 1556, 2914
HphI GGTGA 5 cut(s) 968, 1022, 1820, 2371, 3215
HpyCH4III ACNGT 8 cut(s) 611, 949, 1311, 2119, 2252, 2933, 2978, 3178
HpyCH4IV ACGT 2 cut(s) 1087, 2953
HpyF10VI GCNNNNNNNGC 6 cut(s) 445, 550, 1985, 2264, 2914, 3065
HpyF3I CTNAG 8 cut(s) 289, 393, 1916, 2070, 2398, 2811, 3038, 3044
HpySE526I ACGT 2 cut(s) 1087, 2953
Hsp92I GRCGYC 3 cut(s) 1257, 1800, 1912
HspAI GCGC 1 cut(s) 2337
Kpn2I TCCGGA 1 cut(s) 1555
Ksp22I TGATCA 2 cut(s) 1291, 2755
Lsp1109I GCAGC 7 cut(s) 556, 763, 1069, 1094, 1921, 2365, 2972
LweI GCATC 4 cut(s) 1507, 1997, 2881, 3016
MaeII ACGT 2 cut(s) 1087, 2953
MaeIII GTNAC 9 cut(s) 27, 949, 1147, 1562, 1864, 1946, 2246, 3008, 3203
MfeI CAATTG 2 cut(s) 669, 2825
MflI RGATCY 4 cut(s) 941, 1505, 1960, 2170
MhlI GDGCHC 5 cut(s) 441, 727, 1109, 2048, 2753
MlsI TGGCCA 2 cut(s) 2387, 2588
MluI ACGCGT 1 cut(s) 2451
MluNI TGGCCA 2 cut(s) 2387, 2588
MlyI GAGTC 6 cut(s) 511, 541, 1165, 1828, 1938, 2226
MmeI TCCRAC 1 cut(s) 1012
Mox20I TGGCCA 2 cut(s) 2387, 2588
Mph1103I ATGCAT 2 cut(s) 78, 985
MroI TCCGGA 1 cut(s) 1555
MroXI GAANNNNTTC 1 cut(s) 1666
MscI TGGCCA 2 cut(s) 2387, 2588
MseI TTAA 6 cut(s) 356, 525, 537, 1641, 2634, 3180
MslI CAYNNNNRTG 5 cut(s) 956, 1209, 1266, 1779, 2594
Msp20I TGGCCA 2 cut(s) 2387, 2588
MspA1I CMGCKG 1 cut(s) 2356
MspI CCGG 6 cut(s) 566, 814, 1240, 1400, 1556, 2914
MspR9I CCNGG 6 cut(s) 566, 729, 892, 1480, 1524, 1805
MunI CAATTG 2 cut(s) 669, 2825
Mva1269I GAATGC 2 cut(s) 2558, 2601
MvaI CCWGG 5 cut(s) 729, 892, 1480, 1524, 1805
MvnI CGCG 1 cut(s) 2453
MwoI GCNNNNNNNGC 6 cut(s) 445, 550, 1985, 2264, 2914, 3065
NciI CCSGG 1 cut(s) 566
NheI GCTAGC 1 cut(s) 1001
NmeAIII GCCGAG 2 cut(s) 1018, 1842
NmuCI GTSAC 4 cut(s) 949, 1864, 1946, 3203
NsiI ATGCAT 2 cut(s) 78, 985
NspI RCATGY 2 cut(s) 934, 1326
PaeI GCATGC 1 cut(s) 934
PagI TCATGA 3 cut(s) 1390, 1887, 2866
PasI CCCWGGG 1 cut(s) 1804
PciI ACATGT 1 cut(s) 1322
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PctI GAATGC 2 cut(s) 2558, 2601
PdmI GAANNNNTTC 1 cut(s) 1666
PfeI GAWTC 7 cut(s) 92, 574, 1201, 1836, 1884, 1905, 1970
PflMI CCANNNNNTGG 2 cut(s) 2148, 3200
PinAI ACCGGT 1 cut(s) 1399
PkrI GCNGC 7 cut(s) 546, 753, 1059, 1084, 1936, 2355, 2962
Ple19I CGATCG 2 cut(s) 259, 845
PleI GAGTC 6 cut(s) 511, 540, 1164, 1827, 1937, 2226
PpsI GAGTC 6 cut(s) 511, 540, 1164, 1827, 1937, 2226
PpuMI RGGWCCY 3 cut(s) 562, 1499, 3213
PscI ACATGT 1 cut(s) 1322
PshAI GACNNNNGTC 1 cut(s) 2373
PshBI ATTAAT 1 cut(s) 525
PsiI TTATAA 2 cut(s) 321, 2502
Psp124BI GAGCTC 1 cut(s) 441
Psp5II RGGWCCY 3 cut(s) 562, 1499, 3213
Psp6I CCWGG 5 cut(s) 727, 890, 1478, 1522, 1803
PspEI GGTNACC 1 cut(s) 1562
PspGI CCWGG 5 cut(s) 727, 890, 1478, 1522, 1803
PspOMI GGGCCC 1 cut(s) 723
PspPPI RGGWCCY 3 cut(s) 562, 1499, 3213
PstNI CAGNNNCTG 1 cut(s) 2293
PsuI RGATCY 4 cut(s) 941, 1505, 1960, 2170
PvuI CGATCG 2 cut(s) 259, 845
PvuII CAGCTG 1 cut(s) 2356
RseI CAYNNNNRTG 5 cut(s) 956, 1209, 1266, 1779, 2594
SacI GAGCTC 1 cut(s) 441
SalI GTCGAC 1 cut(s) 2979
SaqAI TTAA 6 cut(s) 356, 525, 537, 1641, 2634, 3180
SatI GCNGC 7 cut(s) 545, 752, 1058, 1083, 1935, 2354, 2961
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 6 cut(s) 511, 541, 1165, 1828, 1938, 2226
ScrFI CCNGG 6 cut(s) 566, 729, 892, 1480, 1524, 1805
SduI GDGCHC 5 cut(s) 441, 727, 1109, 2048, 2753
SfaNI GCATC 4 cut(s) 1507, 1997, 2881, 3016
SfcI CTRYAG 1 cut(s) 945
SinI GGWCC 8 cut(s) 34, 562, 629, 1499, 1771, 2375, 3096, 3213
SmiMI CAYNNNNRTG 5 cut(s) 956, 1209, 1266, 1779, 2594
SmlI CTYRAG 2 cut(s) 83, 1831
SmoI CTYRAG 2 cut(s) 83, 1831
SphI GCATGC 1 cut(s) 934
SsiI CCGC 5 cut(s) 514, 2351, 2510, 2851, 2994
SstI GAGCTC 1 cut(s) 441
StyD4I CCNGG 6 cut(s) 564, 727, 890, 1478, 1522, 1803
StyI CCWWGG 1 cut(s) 2147
TaaI ACNGT 8 cut(s) 611, 949, 1311, 2119, 2252, 2933, 2978, 3178
TaiI ACGT 2 cut(s) 1090, 2956
TaqII GACCGA 1 cut(s) 3113
TatI WGTACW 7 cut(s) 60, 117, 1440, 1535, 2284, 3139, 3277
TfiI GAWTC 7 cut(s) 92, 574, 1201, 1836, 1884, 1905, 1970
Tru1I TTAA 6 cut(s) 356, 525, 537, 1641, 2634, 3180
Tru9I TTAA 6 cut(s) 356, 525, 537, 1641, 2634, 3180
TscAI CASTG 9 cut(s) 213, 1416, 1981, 2023, 2347, 2758, 2843, 2924, 3007
TseFI GTSAC 4 cut(s) 949, 1864, 1946, 3203
TseI GCWGC 7 cut(s) 544, 751, 1057, 1082, 1934, 2353, 2960
Tsp45I GTSAC 4 cut(s) 949, 1864, 1946, 3203
TspGWI ACGGA 2 cut(s) 705, 2125
TspRI CASTG 9 cut(s) 213, 1416, 1981, 2023, 2347, 2758, 2843, 2924, 3007
Van91I CCANNNNNTGG 2 cut(s) 2148, 3200
VpaK11BI GGWCC 8 cut(s) 34, 562, 629, 1499, 1771, 2375, 3096, 3213
VspI ATTAAT 1 cut(s) 525
XagI CCTNNNNNAGG 2 cut(s) 50, 2414
XapI RAATTY 5 cut(s) 7, 674, 1195, 1662, 2322
XbaI TCTAGA 1 cut(s) 872
XceI RCATGY 2 cut(s) 934, 1326
XcmI CCANNNNNNNNNTGG 2 cut(s) 1068, 3155
XmiI GTMKAC 4 cut(s) 2060, 2629, 2707, 2980
XmnI GAANNNNTTC 1 cut(s) 1666
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 2 cut(s) 78, 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.