MD12G1209900.v1.1

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005953
Physical Location & Seq
Reverse (-)
28875456 .. 28884001
8546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1209900.v1.1.491

Sequence Viewer

Length: 3408 bp
ATGTCGTCTTTGAGCAGAGAATTGGTGTTTCTGATACTCCAATTTCTGGAGGAGGAGAAGTTCAACGAGTCCGTACACAGGCTTGAGAAAGAATCCGGATTCTTTTTCAACATGAAGTACTTTGAAGAGAAAATTCAGGCCGGAGAATGGGACGAAGTGGAGAAGTACTTATCAGGATTTACAAAAGTTGATGACAACAGATACTCTATGAAGATATTCTTCGAAATAAGGAAGCAGAAATATCTCGAAGCACTTGATCGGCAAGACAAGCCGAAGGCTGTTGAAATCTTAGTTAGCGATTTGAGAGTGTTCTCCACCTTTAATGAAGAACTTTTCAAAGAAATCACGCAGCTTCTAACTCTTGGAAATTTCAGGGAAAATGAACAGCTTTCCAAGTACGGTGACACGAAAACAGCTCGCAGCATTATGTTGATAGAGCTTAAGAAACTTATAGAAGCAAATCCCCTCTTTCGCGAAAAGCTTGTTTTTCCCGCTCTGAAGACATCAAGACTGCGGACTCTAATCAATCAAAGTTTGAACTGGCAGCACCAGTTATGCAAAAATCCAAGACCAAATCCGGACATCAAAACTTTATTCACTGATCACACATGTACACCTTCAAATGGCCCTCATACTTCTACTCCTACACCTGTTACTCTTCCTGTTGCCGCGGTTGTAAAACCCACTGCTTATCCTTCACTCGGAGCTCATACTCCCTTTTCGGTCGGTGCAGCTGCTGCGAATGCCAATGCTTTAGCAGGTTGGATGGTCAATTCCTCAGTCTCTTCATCTGTGCAAGCAGCCGTTGTTAATGCATCATCCATGCCCGTTCCACAGAACCAAGGCGTTGCAATCTTGAAACATCCAAGAACACCTCCACCAGCCCCAAGTATGGTTGATTATCAGACTGCTGATCATGAATTTATGAAACGATTGCGACCTGTTCAATCTATTGAGGAGGTTGCATATCCAACGTCTAGACAGCAAAGTTCTTGGTCACTGGATGATATACCCAGAACAGTGGCTTTTACCTTGCATCAAGGATCTACTGTCACAAGCATGGACTTTCACCCGTCTCTCCATACGTTGTTGTTAGTTGGTTCTAGTAATGGTGACATTACTCTTTGGGAACTTATGTCGCGGGAGAGGTTGGTTTCAAAGCCGTTCAAGATATGGGATATGACAGCATGTTCACTGCAATTTGAGGCCACAATTGCAAAAGACACACCCATATCTGTCAGCCGTGTTACATGGAGTCCAGATGGGAGTTTCGTGGGGGTTGCATTTACCAAACATTTGATGCACTTGTATGCTTATACTGCACCTAATGATCTACGCCAACATTTGGAGGTTGATGCCCATATCGGTGCTGTGAACGACTTAGCTTTTGCTCATCCAAATAAACAACTGTGTGTTGTGACCTGTGGAGACGACAAGATAATAAAGGTATGGGATTTAACTGGACGAAAGCTATATAACTTTGAAGGTCATGTTGCTCCCGTCTATTCGATCTGTCCTCATTACAAGGAGAGCATTCAGTTCATATTTTCAACTGCTACTGATGGGAAAATAAAGGCCTGGCTGTATGACAACATGGGTTCAAGAGTTGACTATGATGCTCCTGGCCAGTCGTGTACCACAATGCTTTACAGTGCAGATGGAAGTCGATTGTTCTCTTGTGGAACAAGTAAAGAGGGAGACTCGTATCTAGTTGAATGGAATGAAAGTGAAGGAGCAATAAAGAGAACATATTCTGGACTCAGAAAGAAATCAGCAGGCATTGTGCAGTTCGACACAACACGGAATCATTTTTTGGCTGTGGGCGAAGATAGCCAGATCAAGTTTTGGGATATGGACAATAACAATGTTATGACTAGCACAGATGCCGAGGGTGGAATTCCGAGCCTCCCTCGCTTGAGATTCAACAAGGAAGGAAATCTTCTTGCTGTCACAACTGCAGACAACGGATTCAAAATACTTGTAAATGCTGCTGGGGTTAAATCTTTGAAAGCTATCGAGTCCACCGCTTATTTTGAAGGATTAAGGCCTCCCTTTGAATCTACTGCTATCAAGGCATCCGGCTCGGCTTCTGTTACAAATGCTAATGCGGTCAATTGTAAACTGGAAAGGAACTCTCCTGCTAGGTCTACTCCAATTATTCTTGGAGTTGATCACCTCAGTAGAAGTTTTGACAAACCCAGAAGCGTAGAGGATGCAGTGGATAAACCTAAACCCTGGCTGTTGACTGAAATTCAGGATCCTATACAGTGCCGGCAAGTTACCATGCCTGACACAACCGATACTTCTAGCAAGGTTGTCCGACTCCTATATACAAATTCGGGTGCTGGCATATTGGCACTTGGGTCAAATGGTGTGCAGAAGCTGTGGAAGTGGGTCCGTAATGAACAAAACCCAAGTGGAAAGGCCACAGCCAGTGTTGCTCCGCTGCATTGGCAACCAAACAGTGGTCTTCTTATGGCCAACGATGTCTCAGGCGTCAACCTTGAAGAAGCAATTCCATGCATAGCGCTCTCAAAGAACGACTCTTATGTAACGTCAGCTTGCGGCGGAAAGGTCTCGTTATTCAACATGATGACATTCAAGGTGATGACAACATTCATGGCACCTCCTCCTGCTTCCACTTTCCTAGCATTCCATCCTCAGGATAATAACATCCTGGCCATCGGAATGGAAGATTCTACCATCCACATTTACAATGTTCGAGTTGATGAGGTAAAATCAAAATTGAAGGGTCACCAAAAGCGGATAACTGGTTTAGCTTTCTCAACCAATCTTAATATACTCGTTTCGTCCGGTGCTGATGCTCAACTCTGTGTGTGGAGCATTGATACATGGGAGAAGAGGAAGTCGGTTGCAATTCATATGGTTTCTGCAAAGGCAACTGTTGGTGAGACTCGAGTGCAGTTCCATTCCGATCAAATTCGTTTGCTGGTGGTTCACGAGACACAACTAGCAATATATGATGCCTCGAAGATGGATCGCATACGACAGTGGCTTCCACAAGATGCACTTCCTGCGCCAATATCTCATGCAGCATACTCCTGCAACAGCCAACTAATATATGCTGCCTTTTATGACGGTAACATTGGGGTGTTTGATGCCGATAGCCTAAGACTGAGATGCCGTATTGCTCCATCAGCGTACTTACCCCAAGCGGTCTTGAACGGAAGCCAAGCCATGTACCCGCTTGTCGTTGCAGTACATCCACAAGAACCAACCCAGTTTGCTGTTGGGTTGGGTGATGGTTCTGTTAAAGTTATTGAACCCAAAGAATCAGAAGGGAAGTGGGGTTCGTCTCCGCCTTTCGATAACGGAATACCTAATGGAAGAACATCGTCGTCTACCACCAGCAACCACGCGCTCGATCAAATGCAAAGACGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

1136

Amino Acids

125.74

Weight (kDa)

7.0

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 34 2.3e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 1.5e-61 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 1.9e-25 TOPLESS, zinc finger domain
WD40_Prp19 PF24814 332 - 493 7.2e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 349 - 564 2.2e-08 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 390 - 527 1.1e-10 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 392 - 561 2.7e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 412 - 664 6.8e-20 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 412 - 624 3.4e-20 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 458 - 530 7.4e-07 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 464 - 575 1.1e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 465 - 659 1.1e-09 Prp19 WD40 domain
WDR55 PF24796 514 - 656 3.1e-06 WDR55
Beta-prop_WDR3_1st PF25173 517 - 656 2.9e-07 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 828 - 1043 1.2e-19 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 832 - 957 5.1e-13 Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 833 - 998 9.9e-06 MABP1/WDR62 second WD40 domain
WDR55 PF24796 834 - 950 3.5e-06 WDR55
Beta-prop_WDR3_1st PF25173 835 - 961 2.3e-17 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 836 - 1042 1.3e-18 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 836 - 967 2.5e-14 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 837 - 1030 3.8e-17 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 840 - 959 2.7e-12 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 847 - 994 3.3e-11 WDR36/Utp21 second beta-propeller domain
Beta-prop_IFT140_1st PF23383 851 - 954 3e-11 IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 852 - 1001 1e-10 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 857 - 954 6.2e-11 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 889 - 1093 3.1e-11 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 749
AccB1I GGYRCC 1 cut(s) 2623
AccB7I CCANNNNNTGG 3 cut(s) 46, 1345, 2465
AccBSI CCGCTC 1 cut(s) 494
AccI GTMKAC 2 cut(s) 2147, 3362
AccII CGCG 4 cut(s) 474, 671, 1141, 3380
AccIII TCCGGA 2 cut(s) 95, 577
AclWI GGATC 4 cut(s) 1051, 2252, 2265, 3006
AcoI YGGCCR 3 cut(s) 1624, 2478, 2679
AcsI RAATTY 7 cut(s) 132, 367, 920, 1896, 2250, 2335, 2940
AcuI CTGAAG 1 cut(s) 518
AcyI GRCGYC 1 cut(s) 2496
AfaI GTAC 9 cut(s) 75, 119, 167, 398, 613, 1636, 3164, 3203, 3222
AfeI AGCGCT 1 cut(s) 2529
AfiI CCNNNNNNNGG 9 cut(s) 46, 78, 147, 623, 701, 892, 1345, 2465, 2662
AflII CTTAAG 1 cut(s) 440
AflIII ACRYGT 1 cut(s) 608
AjnI CCWGG 4 cut(s) 1577, 1621, 2234, 2676
AjuI GAANNNNNNNTTGG 2 cut(s) 1796, 1828
AloI GAACNNNNNNTCC 4 cut(s) 44, 76, 3295, 3327
Alw21I GWGCWC 1 cut(s) 709
Alw26I GTCTC 9 cut(s) 787, 1080, 1422, 1692, 2494, 2581, 2906, 2957, 3321
AlwI GGATC 4 cut(s) 1051, 2252, 2265, 3006
AlwNI CAGNNNCTG 2 cut(s) 737, 2383
Ama87I CYCGRG 1 cut(s) 2916
Aor13HI TCCGGA 2 cut(s) 95, 577
Aor51HI AGCGCT 1 cut(s) 2529
AoxI GGCC 9 cut(s) 138, 625, 1206, 1575, 1624, 2045, 2424, 2478, 2679
ApoI RAATTY 7 cut(s) 132, 367, 920, 1896, 2250, 2335, 2940
ArsI GACNNNNNNTTYG 2 cut(s) 1370, 1402
Asp700I GAANNNNTTC 3 cut(s) 215, 1750, 2514
AspLEI GCGC 3 cut(s) 2530, 3040, 3382
AspS9I GGNCC 2 cut(s) 626, 2395
AsuHPI GGTGA 8 cut(s) 413, 1061, 1124, 2165, 2617, 2747, 2921, 3272
AsuII TTCGAA 1 cut(s) 222
AvaI CYCGRG 1 cut(s) 2916
AvaII GGWCC 1 cut(s) 2395
AxyI CCTNAGG 1 cut(s) 2661
BalI TGGCCA 3 cut(s) 1626, 2480, 2681
BamHI GGATCC 1 cut(s) 2257
BanI GGYRCC 1 cut(s) 2623
BanII GRGCYC 1 cut(s) 709
BauI CACGAG 1 cut(s) 2960
BbsI GAAGAC 2 cut(s) 506, 2462
Bbv12I GWGCWC 1 cut(s) 709
BceAI ACGGC 4 cut(s) 788, 1147, 1227, 3129
BcgI CGANNNNNNTGC 2 cut(s) 2064, 2098
BciT130I CCWGG 4 cut(s) 1579, 1623, 2236, 2678
BclI TGATCA 3 cut(s) 601, 913, 2170
BcoDI GTCTC 9 cut(s) 787, 1080, 1422, 1692, 2494, 2581, 2906, 2957, 3321
BfaI CTAG 8 cut(s) 978, 1104, 1709, 1875, 2142, 2307, 2648, 2972
BfmI CTRYAG 1 cut(s) 1956
BfoI RGCGCY 1 cut(s) 2531
BfrI CTTAAG 1 cut(s) 440
BfuAI ACCTGC 1 cut(s) 749
BmcAI AGTACT 2 cut(s) 119, 167
Bme1390I CCNGG 4 cut(s) 1579, 1623, 2236, 2678
Bme18I GGWCC 1 cut(s) 2395
BmeT110I CYCGRG 1 cut(s) 2916
BmgT120I GGNCC 2 cut(s) 626, 2395
BmiI GGNNCC 3 cut(s) 2259, 2396, 2625
BmrFI CCNGG 4 cut(s) 1579, 1623, 2236, 2678
BmrI ACTGGG 1 cut(s) 3235
BmuI ACTGGG 1 cut(s) 3235
BpiI GAAGAC 2 cut(s) 506, 2462
BplI GAGNNNNNCTC 4 cut(s) 1685, 1717, 1894, 1926
BpmI CTGGAG 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 222
BpuEI CTTGAG 2 cut(s) 104, 1936
BsaHI GRCGYC 1 cut(s) 2496
BsaI GGTCTC 1 cut(s) 2581
BsaJI CCNNGG 4 cut(s) 669, 841, 1887, 2234
BsaWI WCCGGW 3 cut(s) 95, 577, 2813
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 625, 655
Bsc4I CCNNNNNNNGG 9 cut(s) 46, 78, 147, 623, 701, 892, 1345, 2465, 2662
Bse118I RCCGGY 1 cut(s) 2271
Bse1I ACTGG 9 cut(s) 545, 550, 1005, 1465, 1627, 2127, 2433, 2776, 3241
Bse21I CCTNAGG 1 cut(s) 2661
BseAI TCCGGA 2 cut(s) 95, 577
BseBI CCWGG 4 cut(s) 1579, 1623, 2236, 2678
BseDI CCNNGG 4 cut(s) 669, 841, 1887, 2234
BseLI CCNNNNNNNGG 9 cut(s) 46, 78, 147, 623, 701, 892, 1345, 2465, 2662
BseMII CTCAG 6 cut(s) 792, 1774, 2191, 2505, 2675, 3128
BseNI ACTGG 9 cut(s) 545, 550, 1005, 1465, 1627, 2127, 2433, 2776, 3241
BseRI GAGGAG 4 cut(s) 65, 68, 971, 2619
BseYI CCCAGC 1 cut(s) 1991
BsgI GTGCAG 6 cut(s) 750, 1305, 1674, 1805, 2396, 2942
Bsh1236I CGCG 4 cut(s) 474, 671, 1141, 3380
Bsh1285I CGRYCG 1 cut(s) 726
BshFI GGCC 9 cut(s) 140, 627, 1208, 1577, 1626, 2047, 2426, 2480, 2681
BshNI GGYRCC 1 cut(s) 2623
BsiEI CGRYCG 1 cut(s) 726
BsiHKAI GWGCWC 1 cut(s) 709
BsiHKCI CYCGRG 1 cut(s) 2916
BsiSI CCGG 6 cut(s) 96, 141, 578, 2079, 2272, 2814
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 9 cut(s) 46, 78, 147, 623, 701, 892, 1345, 2465, 2662
BsmAI GTCTC 9 cut(s) 787, 1080, 1422, 1692, 2494, 2581, 2906, 2957, 3321
BsmBI CGTCTC 3 cut(s) 1080, 1422, 3321
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 3 cut(s) 748, 1533, 2651
BsnI GGCC 9 cut(s) 140, 627, 1208, 1577, 1626, 2047, 2426, 2480, 2681
Bso31I GGTCTC 1 cut(s) 2581
BsoBI CYCGRG 1 cut(s) 2916
Bsp119I TTCGAA 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 709
Bsp13I TCCGGA 2 cut(s) 95, 577
Bsp1407I TGTACA 1 cut(s) 611
Bsp68I TCGCGA 1 cut(s) 474
BspANI GGCC 9 cut(s) 140, 627, 1208, 1577, 1626, 2047, 2426, 2480, 2681
BspCNI CTCAG 6 cut(s) 791, 1773, 2190, 2504, 2674, 3129
BspEI TCCGGA 2 cut(s) 95, 577
BspFNI CGCG 4 cut(s) 474, 671, 1141, 3380
BspHI TCATGA 1 cut(s) 916
BspLI GGNNCC 3 cut(s) 2259, 2396, 2625
BspMAI CTGCAG 1 cut(s) 1960
BspMI ACCTGC 1 cut(s) 749
BspPI GGATC 4 cut(s) 1051, 2252, 2265, 3006
BspT104I TTCGAA 1 cut(s) 222
BspT107I GGYRCC 1 cut(s) 2623
BspTI CTTAAG 1 cut(s) 440
BspTNI GGTCTC 1 cut(s) 2581
BsrBI CCGCTC 1 cut(s) 494
BsrFI RCCGGY 1 cut(s) 2271
BsrGI TGTACA 1 cut(s) 611
BsrI ACTGG 9 cut(s) 545, 550, 1005, 1465, 1627, 2127, 2433, 2776, 3241
BssAI RCCGGY 1 cut(s) 2271
BssECI CCNNGG 4 cut(s) 669, 841, 1887, 2234
BssNI GRCGYC 1 cut(s) 2496
BssSI CACGAG 1 cut(s) 2960
BssT1I CCWWGG 1 cut(s) 841
Bst2BI CACGAG 1 cut(s) 2960
Bst2UI CCWGG 4 cut(s) 1579, 1623, 2236, 2678
Bst6I CTCTTC 4 cut(s) 120, 663, 790, 2855
BstACI GRCGYC 1 cut(s) 2496
BstAFI CTTAAG 1 cut(s) 440
BstAPI GCANNNNNTGC 2 cut(s) 737, 3035
BstAUI TGTACA 1 cut(s) 611
BstBI TTCGAA 1 cut(s) 222
BstC8I GCNNGC 6 cut(s) 418, 798, 1777, 2273, 2347, 2563
BstDEI CTNAG 9 cut(s) 289, 778, 1381, 1760, 2177, 2491, 2661, 3131, 3137
BstDSI CCRYGG 1 cut(s) 669
BstEII GGTNACC 1 cut(s) 2753
BstFNI CGCG 4 cut(s) 474, 671, 1141, 3380
BstH2I RGCGCY 1 cut(s) 2531
BstHHI GCGC 3 cut(s) 2530, 3040, 3382
BstMAI GTCTC 9 cut(s) 787, 1080, 1422, 1692, 2494, 2581, 2906, 2957, 3321
BstMCI CGRYCG 1 cut(s) 726
BstNI CCWGG 4 cut(s) 1579, 1623, 2236, 2678
BstNSI RCATGY 2 cut(s) 612, 1191
BstPI GGTNACC 1 cut(s) 2753
BstSCI CCNGG 4 cut(s) 1577, 1621, 2234, 2676
BstSFI CTRYAG 1 cut(s) 1956
BstUI CGCG 4 cut(s) 474, 671, 1141, 3380
BstV2I GAAGAC 2 cut(s) 506, 2462
BstX2I RGATCY 2 cut(s) 1043, 2257
BstXI CCANNNNNNTGG 2 cut(s) 1021, 2689
BstYI RGATCY 2 cut(s) 1043, 2257
Bsu36I CCTNAGG 1 cut(s) 2661
BsuRI GGCC 9 cut(s) 140, 627, 1208, 1577, 1626, 2047, 2426, 2480, 2681
BtgI CCRYGG 1 cut(s) 669
BtsI GCAGTG 3 cut(s) 684, 1193, 2223
BtuMI TCGCGA 1 cut(s) 474
BveI ACCTGC 1 cut(s) 749
Cac8I GCNNGC 6 cut(s) 418, 798, 1777, 2273, 2347, 2563
CaiI CAGNNNCTG 2 cut(s) 737, 2383
CciI TCATGA 1 cut(s) 916
CfoI GCGC 3 cut(s) 2530, 3040, 3382
Cfr10I RCCGGY 1 cut(s) 2271
Cfr13I GGNCC 2 cut(s) 626, 2395
Cfr42I CCGCGG 1 cut(s) 672
CseI GACGC 1 cut(s) 2485
Csp6I GTAC 9 cut(s) 74, 118, 166, 397, 612, 1635, 3163, 3202, 3221
CviQI GTAC 9 cut(s) 74, 118, 166, 397, 612, 1635, 3163, 3202, 3221
DdeI CTNAG 9 cut(s) 289, 778, 1381, 1760, 2177, 2491, 2661, 3131, 3137
EaeI YGGCCR 3 cut(s) 1624, 2478, 2679
Eam1104I CTCTTC 4 cut(s) 120, 663, 790, 2855
EarI CTCTTC 4 cut(s) 120, 663, 790, 2855
EciI GGCGGA 2 cut(s) 2583, 3309
Ecl136II GAGCTC 1 cut(s) 707
Eco130I CCWWGG 1 cut(s) 841
Eco147I AGGCCT 2 cut(s) 1577, 2047
Eco24I GRGCYC 1 cut(s) 709
Eco31I GGTCTC 1 cut(s) 2581
Eco47I GGWCC 1 cut(s) 2395
Eco47III AGCGCT 1 cut(s) 2529
Eco53kI GAGCTC 1 cut(s) 707
Eco57I CTGAAG 1 cut(s) 518
Eco81I CCTNAGG 1 cut(s) 2661
Eco88I CYCGRG 1 cut(s) 2916
Eco91I GGTNACC 1 cut(s) 2753
EcoICRI GAGCTC 1 cut(s) 707
EcoO65I GGTNACC 1 cut(s) 2753
EcoRI GAATTC 1 cut(s) 1896
EcoRII CCWGG 4 cut(s) 1577, 1621, 2234, 2676
EcoT14I CCWWGG 1 cut(s) 841
EcoT22I ATGCAT 2 cut(s) 817, 2525
EcoT38I GRGCYC 1 cut(s) 709
ErhI CCWWGG 1 cut(s) 841
Esp3I CGTCTC 3 cut(s) 1080, 1422, 3321
FalI AAGNNNNNCTT 6 cut(s) 203, 235, 1923, 1955, 3015, 3047
FaqI GGGAC 1 cut(s) 164
FauI CCCGC 3 cut(s) 499, 1134, 3213
FauNDI CATATG 1 cut(s) 2883
FbaI TGATCA 3 cut(s) 601, 913, 2170
FblI GTMKAC 2 cut(s) 2147, 3362
FriOI GRGCYC 1 cut(s) 709
FspBI CTAG 8 cut(s) 978, 1104, 1709, 1875, 2142, 2307, 2648, 2972
GlaI GCGC 3 cut(s) 2529, 3039, 3381
GsaI CCCAGC 1 cut(s) 1995
GsuI CTGGAG 1 cut(s) 68
HaeII RGCGCY 1 cut(s) 2531
HaeIII GGCC 9 cut(s) 140, 627, 1208, 1577, 1626, 2047, 2426, 2480, 2681
HapII CCGG 6 cut(s) 96, 141, 578, 2079, 2272, 2814
HgaI GACGC 1 cut(s) 2485
HhaI GCGC 3 cut(s) 2530, 3040, 3382
Hin1I GRCGYC 1 cut(s) 2496
Hin6I GCGC 3 cut(s) 2528, 3038, 3380
HinP1I GCGC 3 cut(s) 2528, 3038, 3380
HincII GTYRAC 3 cut(s) 1609, 2244, 2500
HindII GTYRAC 3 cut(s) 1609, 2244, 2500
HindIII AAGCTT 1 cut(s) 479
HpaII CCGG 6 cut(s) 96, 141, 578, 2079, 2272, 2814
HphI GGTGA 8 cut(s) 413, 1061, 1124, 2165, 2617, 2747, 2921, 3272
Hpy99I CGWCG 1 cut(s) 3361
HpyAV CCTTC 9 cut(s) 268, 627, 705, 1478, 1724, 1925, 2030, 2743, 3293
HpyCH4IV ACGT 3 cut(s) 974, 1085, 2555
HpyF3I CTNAG 9 cut(s) 289, 778, 1381, 1760, 2177, 2491, 2661, 3131, 3137
HpySE526I ACGT 3 cut(s) 974, 1085, 2555
Hsp92I GRCGYC 1 cut(s) 2496
HspAI GCGC 3 cut(s) 2528, 3038, 3380
Kpn2I TCCGGA 2 cut(s) 95, 577
KroI GCCGGC 1 cut(s) 2271
KroNI GCCGGC 1 cut(s) 2273
Ksp22I TGATCA 3 cut(s) 601, 913, 2170
KspI CCGCGG 1 cut(s) 672
LmnI GCTCC 7 cut(s) 704, 1501, 1624, 1733, 2446, 2841, 3157
MaeI CTAG 8 cut(s) 978, 1104, 1709, 1875, 2142, 2307, 2648, 2972
MaeII ACGT 3 cut(s) 974, 1085, 2555
MbiI CCGCTC 1 cut(s) 494
MfeI CAATTG 2 cut(s) 1212, 2113
MflI RGATCY 2 cut(s) 1043, 2257
MhlI GDGCHC 1 cut(s) 709
MlsI TGGCCA 3 cut(s) 1626, 2480, 2681
MluNI TGGCCA 3 cut(s) 1626, 2480, 2681
MlyI GAGTC 9 cut(s) 77, 511, 1264, 1694, 1752, 2027, 2316, 2537, 2908
MmeI TCCRAC 3 cut(s) 743, 995, 2344
Mox20I TGGCCA 3 cut(s) 1626, 2480, 2681
Mph1103I ATGCAT 2 cut(s) 817, 2525
MroI TCCGGA 2 cut(s) 95, 577
MroNI GCCGGC 1 cut(s) 2271
MroXI GAANNNNTTC 3 cut(s) 215, 1750, 2514
MscI TGGCCA 3 cut(s) 1626, 2480, 2681
MseI TTAA 8 cut(s) 321, 441, 810, 1457, 1998, 2042, 2796, 3273
MslI CAYNNNNRTG 5 cut(s) 1058, 1308, 1365, 2687, 3110
Msp20I TGGCCA 3 cut(s) 1626, 2480, 2681
MspA1I CMGCKG 3 cut(s) 671, 734, 2446
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 6 cut(s) 96, 141, 578, 2079, 2272, 2814
MspR9I CCNGG 4 cut(s) 1579, 1623, 2236, 2678
MunI CAATTG 2 cut(s) 1212, 2113
Mva1269I GAATGC 3 cut(s) 748, 1533, 2651
MvaI CCWGG 4 cut(s) 1579, 1623, 2236, 2678
MvnI CGCG 4 cut(s) 474, 671, 1141, 3380
NaeI GCCGGC 1 cut(s) 2273
NdeI CATATG 1 cut(s) 2883
NgoMIV GCCGGC 1 cut(s) 2271
NlaIV GGNNCC 3 cut(s) 2259, 2396, 2625
NmeAIII GCCGAG 2 cut(s) 1912, 2063
NmuCI GTSAC 7 cut(s) 401, 996, 1051, 1112, 1417, 1948, 2753
NruI TCGCGA 1 cut(s) 474
NsiI ATGCAT 2 cut(s) 817, 2525
NspI RCATGY 2 cut(s) 612, 1191
NspV TTCGAA 1 cut(s) 222
PaeR7I CTCGAG 1 cut(s) 2916
PagI TCATGA 1 cut(s) 916
PceI AGGCCT 2 cut(s) 1577, 2047
PciI ACATGT 1 cut(s) 608
PctI GAATGC 3 cut(s) 748, 1533, 2651
PdiI GCCGGC 1 cut(s) 2273
PdmI GAANNNNTTC 3 cut(s) 215, 1750, 2514
PfeI GAWTC 8 cut(s) 92, 99, 1804, 1920, 1968, 2057, 2696, 3293
PflMI CCANNNNNTGG 3 cut(s) 46, 1345, 2465
PleI GAGTC 9 cut(s) 76, 511, 1263, 1694, 1752, 2026, 2316, 2537, 2908
PpsI GAGTC 9 cut(s) 76, 511, 1263, 1694, 1752, 2026, 2316, 2537, 2908
PscI ACATGT 1 cut(s) 608
Psp124BI GAGCTC 1 cut(s) 709
Psp6I CCWGG 4 cut(s) 1577, 1621, 2234, 2676
PspEI GGTNACC 1 cut(s) 2753
PspFI CCCAGC 1 cut(s) 1991
PspGI CCWGG 4 cut(s) 1577, 1621, 2234, 2676
PspN4I GGNNCC 3 cut(s) 2259, 2396, 2625
PspPI GGNCC 2 cut(s) 626, 2395
PspXI VCTCGAGB 1 cut(s) 2916
PstI CTGCAG 1 cut(s) 1960
PstNI CAGNNNCTG 2 cut(s) 737, 2383
PsuI RGATCY 2 cut(s) 1043, 2257
PvuII CAGCTG 1 cut(s) 734
RruI TCGCGA 1 cut(s) 474
RsaI GTAC 9 cut(s) 75, 119, 167, 398, 613, 1636, 3164, 3203, 3222
RsaNI GTAC 9 cut(s) 74, 118, 166, 397, 612, 1635, 3163, 3202, 3221
RseI CAYNNNNRTG 5 cut(s) 1058, 1308, 1365, 2687, 3110
SacI GAGCTC 1 cut(s) 709
SacII CCGCGG 1 cut(s) 672
SaqAI TTAA 8 cut(s) 321, 441, 810, 1457, 1998, 2042, 2796, 3273
Sau96I GGNCC 2 cut(s) 626, 2395
ScaI AGTACT 2 cut(s) 119, 167
SchI GAGTC 9 cut(s) 77, 511, 1264, 1694, 1752, 2027, 2316, 2537, 2908
ScrFI CCNGG 4 cut(s) 1579, 1623, 2236, 2678
SduI GDGCHC 1 cut(s) 709
SfcI CTRYAG 1 cut(s) 1956
Sfr274I CTCGAG 1 cut(s) 2916
Sfr303I CCGCGG 1 cut(s) 672
SfuI TTCGAA 1 cut(s) 222
SgrBI CCGCGG 1 cut(s) 672
SinI GGWCC 1 cut(s) 2395
SlaI CTCGAG 1 cut(s) 2916
SmiMI CAYNNNNRTG 5 cut(s) 1058, 1308, 1365, 2687, 3110
SmlI CTYRAG 4 cut(s) 83, 440, 1915, 2916
SmoI CTYRAG 4 cut(s) 83, 440, 1915, 2916
SseBI AGGCCT 2 cut(s) 1577, 2047
SspMI CTAG 8 cut(s) 978, 1104, 1709, 1875, 2142, 2307, 2648, 2972
SstI GAGCTC 1 cut(s) 709
StuI AGGCCT 2 cut(s) 1577, 2047
StyD4I CCNGG 4 cut(s) 1577, 1621, 2234, 2676
StyI CCWWGG 1 cut(s) 841
TaiI ACGT 3 cut(s) 977, 1088, 2558
TaqII GACCGA 1 cut(s) 712
TatI WGTACW 4 cut(s) 117, 165, 611, 3220
TauI GCSGC 2 cut(s) 671, 2568
TfiI GAWTC 8 cut(s) 92, 99, 1804, 1920, 1968, 2057, 2696, 3293
Tru1I TTAA 8 cut(s) 321, 441, 810, 1457, 1998, 2042, 2796, 3273
Tru9I TTAA 8 cut(s) 321, 441, 810, 1457, 1998, 2042, 2796, 3273
TseFI GTSAC 7 cut(s) 401, 996, 1051, 1112, 1417, 1948, 2753
Tsp45I GTSAC 7 cut(s) 401, 996, 1051, 1112, 1417, 1948, 2753
TspGWI ACGGA 6 cut(s) 61, 1816, 1980, 2387, 3201, 3348
Van91I CCANNNNNTGG 3 cut(s) 46, 1345, 2465
Vha464I CTTAAG 1 cut(s) 440
VpaK11BI GGWCC 1 cut(s) 2395
XapI RAATTY 7 cut(s) 132, 367, 920, 1896, 2250, 2335, 2940
XbaI TCTAGA 1 cut(s) 977
XceI RCATGY 2 cut(s) 612, 1191
XcmI CCANNNNNNNNNTGG 1 cut(s) 3248
XhoI CTCGAG 1 cut(s) 2916
XmiI GTMKAC 2 cut(s) 2147, 3362
XmnI GAANNNNTTC 3 cut(s) 215, 1750, 2514
XspI CTAG 8 cut(s) 978, 1104, 1709, 1875, 2142, 2307, 2648, 2972
ZrmI AGTACT 2 cut(s) 119, 167
Zsp2I ATGCAT 2 cut(s) 817, 2525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.