MD04G1197000.v1.1

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005953
Physical Location & Seq
Reverse (-)
28516307 .. 28525382
9076 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1197000.v1.1.491

Sequence Viewer

Length: 3408 bp
ATGTCGTCTTTGAGCAGAGAATTGGTGTTTCTGATACTCCAATTTCTGGAAGAGGAGAAGTTCAAGGAGTCCGTACACAGGCTTGAGAAAGAATCAGGATTCTTTTTCAACATGAAGTACTTTGAAGAAAAAATTCAGGCCGGAGAATGGGACGAAGTGGAGAAGTACTTATCAGGATTTACAAAAGTTGATGACAACAGATACTCTATGAAGATATTTTTCGAAATAAGGAAGCAGAAATATCTGGAAGCACTTGATCGGCAAGACAAGCCGAAGGCTGTTGAAATCTTAGTGAATGATTTGAGAGTGTTCTCCACATTTAATGAAGAACTTTTCAAAGAAATCACACAGCTTCTAACTCTTGGAAATTTCAGGGAAAATGAACAGCTTTCCAAGTATGGGGACACCAAAACGGCTCGCAGCATTATGTTGATAGAGCTTAAGAAACTTATAGAAGCAAATCCCCTCTTTCGTGATAAGCTTGTTTTTCCCGCTCTGAAGGCATCGAGACTGCGGACTCTAATCAATCAAAGTTTGAACTGGCAGCACCAGTTATGCAAAAATCCAAGAGTAAATCCGGACATCAAAACTTTATTTACTGATCACACATGTACAGCTCCAAATGGCCCTCATACTTCTACGCCTACACCTGTTACTCCTCCAGTTGCCGCTGTTGCAAAACCCACTGCTTATCCTTCGCTCGGATCTCATGCTCCCTTTTCGGTCGGTGCAGCTGCTGCGAATTCTAATGCTTTAGCAGGTTGGATGGCCAATTCCTCAGTCTCTTCATCTGTGCAAGCAGCCGTTGTTAATGCATCATCCATGCCCATTCCACAGAACCAAGGCGTTGCAATCTTGAAACGTCCAAGAACACCTCCAGCTGCTCCAAGTATGGTTGATCATCAGAGTGCTGATCATGAACTTATGAAACGACTACGACCTGTTCAATCTATTGAGGAGGTTGCATATCCAACGTCTAGACAACAAACTTCTTGGTCACTGGATGATCTACCCAGAACAGTGGCTTTTAACCTGCATCAAGGATCTACTGTCACAAGCATGGATTTTCACCCGGCTCTCCATACGTTGTTGCTAGTTGGTTCTAATAATGGCGACATTACTCTTTGGGAACTTATGTCGCGGGAGAGGTTGGTTTCAAAGCCATTCAAGATATGGGATATGACAAAATGTACACTGCAATTTCAGGCCACAATTGGCAAGGACACACCCGTATCTGTCAGCCGTGTGACGTGGAGTCAAGATGGGAGTTTTGTGGGGGTTGCATTTACCAAACATTTGATTCACTTGTACGTTTATACTGGACCTAATGATCTACGCCAACATTTGGAGGTTGATGCCCATAACGGTGCTGTGAACGACTTAGCATTTGCTCATCCAAATAAACAACTGTGTGTTGTGACCTGTGGAGACGATAAGATAATAAAGGTATGGGATTTAACCGGACGAAAGCTATATAACTTTGAAGGTCATGATGCTCCCGTCTATTCGATATGTCCTCATTACAAGGAGAACATTCAGTTCATATTTTCAACTGCTACTGATGGGAAAATAAAGGCCTGGCTGTACGACAACTTGGGTTCAAGAGTTGACTATGATGCTCCTGGCCAGTCGTGTACCACAATGCTTTACAGTGCAGATGGAAGTCGATTGTTCTCTTGTGGAACAAGTAAAGAGGGAGACTCGTATCTAGTTGAATGGAATGAAAGTGAAGGAGCAATAAAGAGAACATATTCTGGACTCAGGAAGAAATCAGCAGGCGTTGTGCAATTCGACACAACACAAAATCATTTTTTGGCAGTTGGTGAAGATAGCCAGATAAAGTTTTGGGATATGGACAATAACAATGTTATGACTACCATAGACGCTGAGGGTGGACTTCCGAGCCTCCCTCGCTTGAGATTCAACAAGGAAGGAAATCTTCTTGCTGTCACAACTGCAGACAACGGATTCAAAATACTTGTAAATGCTGCTGGAGTTAAAACTTTGAAAGCTATCGAGTCCACCACATCGTTTGAGGGATTAAGGCCTCCCATTGAATCCACTGCTATCAAGGCATCCGGCTCGGCTTCTGTTACAAATGCAAATGCGGTCAATTGTAAAGTGGAAAGGAGCTCTCCTGCTAGGTCTACTCCAATTATTCTTGGAGTTGATCACCTCAGTAGAAGTTTCGACAAACTGAGAAGCGTAGAGGATGCAGTGGATAAACCTAAACCCTGGCTGTTGACTGAAATTCAGGATCCTATACAGTGCCGGCTAGTTACCATGCCTGACACAACCGATACTTCTAGCAAGGTCGTTCGACTCCTATATACAAATTCGGGTGCTGGCATTTTGGCACTTGGGTCAAATGGTGTGCAGAAGCTGTGGAAGTGGGTCCGTAATGACCAAAATCCAAGTGGAAAGGCCACTGCCAGTGTTGCTCCGCTGCATTGGCAACCAAACAGTGGTCTTCTTATGGCCAATGACATCTCGGGCGTCAACCTTGAAGAAGCAATTCCATGCATAGCGCTCTCAAAGAATGACTCTTATGTAATGTCTGCTTGTGGTGGAAAGGTCTCGTTATTCAACATGATGACATTCAAGGTAATGACAACATTCATGGCGCCTCCTCCTGCTTCGACTTTCCTAGCATTCCATCCTCAGGATAATAACATCCTCGCCATCGGAATGGAAGATTCTACCATCCACATTTACAATGTTCGAGTTGATGAGGTGAAATCAAAATTGAAGAGTCACCAAAAGCGGATAACTGGTTTAGCTTTCTCAACCAATCTTAATATACTCGTTTCATCCGGTGCTGATGCTCAACTCTGTGTGTGGAGCATTGATACATGGGAGAAGCGGAAATCGGTTGCAATTCATATGGTTTCTGCAAAGGCAACTGTTGGTGAGACTCGAGTGCAGTTCCATTCCGATCAAATTCACTTGCTGGTGGTTCACGAGACGCAACTAGCAATATATGATGCCTCAAAAATGGAACGCATCCGACAGTGGCTTCCACAAGATCCACTTCCTGCACCAATATCTCATGCAGCATACTCCTGCAACAGCCAACTAATATATGCCGCCTTTTGTGACGGTAACATTGGGGTGTTTGATGCCGATAGCCTAAGACTCAGATGCCGTATTGCTCCATCAGCATACATACCTCAAGCAGCCTTGAACGGAAGCCAAGCCATGTACCCGCTGGTAGTTGCGGTACATCCACAAGAACCAACCCAGTTTGCTGTTGGGTTGGGCGATGGTTCTGTTAAAGTTATTGAACCCAACGAATCAGAAGGGAAGTGGGGTTCGTCTCCACCTGTTGATAACGGAATACCTAATGGAAGAACATCGTCGTCTACCACCAGCAACCACGCCCTCGATCAAATGCAAAGACGGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

1136

Amino Acids

125.52

Weight (kDa)

7.01

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 5 - 34 3e-11 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 4.2e-62 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 9.1e-25 TOPLESS, zinc finger domain
Beta-prop_WDR3_2nd PF25172 349 - 564 1.3e-09 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 390 - 527 1.9e-12 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 390 - 603 3.6e-08 Echinoderm microtubule-associated protein first beta-propeller
WD40_CDC20-Fz PF24807 391 - 561 9.8e-07 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 412 - 665 4e-21 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 412 - 626 4.7e-21 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 458 - 530 2.6e-07 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 464 - 575 2.8e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 465 - 659 8.7e-11 Prp19 WD40 domain
EIF3I PF24805 472 - 677 7.3e-06 EIF3I
WDR55 PF24796 500 - 656 1.7e-06 WDR55
Beta-prop_WDR3_1st PF25173 520 - 656 1.8e-07 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 828 - 1043 1.4e-18 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 832 - 957 1.2e-12 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 834 - 950 1.2e-06 WDR55
Beta-prop_WDR5 PF25175 836 - 1042 7.4e-18 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 836 - 961 2.2e-16 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 836 - 967 1.9e-14 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 836 - 1030 3.9e-17 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 838 - 994 1.8e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 840 - 959 1e-12 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 846 - 962 1.2e-10 THOC3 beta-propeller domain
Beta-prop_IFT140_1st PF23383 852 - 954 2.2e-11 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 856 - 955 1.9e-11 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 858 - 990 2.3e-10 WDR90/POC16, second beta-propeller
WDR55 PF24796 883 - 1041 4.1e-06 WDR55
Beta-prop_THOC3 PF25174 914 - 1093 2e-06 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 925 - 1043 8.4e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 749, 1041
AccB1I GGYRCC 1 cut(s) 2623
AccB7I CCANNNNNTGG 3 cut(s) 46, 1345, 2465
AccBSI CCGCTC 1 cut(s) 494
AccI GTMKAC 2 cut(s) 2147, 3362
AccII CGCG 1 cut(s) 1141
AccIII TCCGGA 1 cut(s) 577
AclWI GGATC 5 cut(s) 712, 1051, 2252, 2265, 3020
AcoI YGGCCR 3 cut(s) 768, 1624, 2478
AcsI RAATTY 6 cut(s) 132, 367, 742, 2250, 2335, 2940
AcuI CTGAAG 1 cut(s) 518
AcyI GRCGYC 2 cut(s) 2496, 2624
AfeI AGCGCT 1 cut(s) 2529
AfiI CCNNNNNNNGG 8 cut(s) 46, 78, 147, 399, 701, 1345, 2465, 2662
AflII CTTAAG 1 cut(s) 440
AflIII ACRYGT 1 cut(s) 608
AhdI GACNNNNNGTC 1 cut(s) 1254
AjiI CACGTC 1 cut(s) 1251
AjnI CCWGG 3 cut(s) 1577, 1621, 2234
AloI GAACNNNNNNTCC 2 cut(s) 3295, 3327
Alw21I GWGCWC 1 cut(s) 2135
Alw26I GTCTC 8 cut(s) 502, 787, 1422, 1692, 2581, 2906, 2957, 3321
AlwI GGATC 5 cut(s) 712, 1051, 2252, 2265, 3020
AlwNI CAGNNNCTG 2 cut(s) 737, 2383
Ama87I CYCGRG 2 cut(s) 2491, 2916
Aor13HI TCCGGA 1 cut(s) 577
Aor51HI AGCGCT 1 cut(s) 2529
AoxI GGCC 9 cut(s) 138, 625, 768, 1206, 1575, 1624, 2045, 2424, 2478
ApoI RAATTY 6 cut(s) 132, 367, 742, 2250, 2335, 2940
ArsI GACNNNNNNTTYG 2 cut(s) 1370, 1402
Asp700I GAANNNNTTC 3 cut(s) 132, 1750, 2514
AspLEI GCGC 2 cut(s) 2530, 2626
AspS9I GGNCC 3 cut(s) 626, 1322, 2395
AsuC2I CCSGG 1 cut(s) 1073
AsuHPI GGTGA 6 cut(s) 1061, 1835, 2165, 2746, 2747, 2921
AsuII TTCGAA 1 cut(s) 222
AvaI CYCGRG 2 cut(s) 2491, 2916
AvaII GGWCC 2 cut(s) 1322, 2395
AxyI CCTNAGG 1 cut(s) 2661
BaeI ACNNNNGTAYC 2 cut(s) 1215, 1248
BalI TGGCCA 3 cut(s) 770, 1626, 2480
BamHI GGATCC 1 cut(s) 2257
BanI GGYRCC 1 cut(s) 2623
BanII GRGCYC 1 cut(s) 2135
BauI CACGAG 1 cut(s) 2960
BbsI GAAGAC 1 cut(s) 2462
Bbv12I GWGCWC 1 cut(s) 2135
BbvCI CCTCAGC 1 cut(s) 1887
BccI CCATC 9 cut(s) 760, 1256, 1556, 1652, 2664, 2690, 2711, 3163, 3257
BceAI ACGGC 4 cut(s) 429, 788, 1227, 3129
BcgI CGANNNNNNTGC 2 cut(s) 2064, 2098
BciT130I CCWGG 3 cut(s) 1579, 1623, 2236
BclI TGATCA 4 cut(s) 601, 898, 913, 2170
BcnI CCSGG 1 cut(s) 1073
BcoDI GTCTC 8 cut(s) 502, 787, 1422, 1692, 2581, 2906, 2957, 3321
BfaI CTAG 8 cut(s) 978, 1094, 1709, 2142, 2276, 2307, 2648, 2972
BfmI CTRYAG 1 cut(s) 1956
BfoI RGCGCY 2 cut(s) 2531, 2627
BfrI CTTAAG 1 cut(s) 440
BfuAI ACCTGC 2 cut(s) 749, 1041
BmcAI AGTACT 2 cut(s) 119, 167
Bme1390I CCNGG 4 cut(s) 1073, 1579, 1623, 2236
Bme18I GGWCC 2 cut(s) 1322, 2395
BmeRI GACNNNNNGTC 1 cut(s) 1254
BmeT110I CYCGRG 2 cut(s) 2491, 2916
BmgBI CACGTC 1 cut(s) 1251
BmgT120I GGNCC 3 cut(s) 626, 1322, 2395
BmiI GGNNCC 3 cut(s) 2259, 2396, 2625
BmrFI CCNGG 4 cut(s) 1073, 1579, 1623, 2236
BmrI ACTGGG 1 cut(s) 3235
BmuI ACTGGG 1 cut(s) 3235
BpiI GAAGAC 1 cut(s) 2462
BplI GAGNNNNNCTC 4 cut(s) 1685, 1717, 1894, 1926
BpmI CTGGAG 3 cut(s) 645, 861, 2013
Bpu10I CCTNAGC 1 cut(s) 1887
Bpu14I TTCGAA 1 cut(s) 222
BpuEI CTTGAG 3 cut(s) 104, 1936, 3156
BpuMI CCSGG 1 cut(s) 1073
BsaHI GRCGYC 2 cut(s) 2496, 2624
BsaI GGTCTC 1 cut(s) 2581
BsaJI CCNNGG 2 cut(s) 841, 2234
BsaWI WCCGGW 3 cut(s) 577, 1460, 2813
Bsc4I CCNNNNNNNGG 8 cut(s) 46, 78, 147, 399, 701, 1345, 2465, 2662
Bse118I RCCGGY 1 cut(s) 2271
Bse1I ACTGG 9 cut(s) 545, 550, 662, 1005, 1324, 1627, 2433, 2776, 3241
Bse21I CCTNAGG 1 cut(s) 2661
BseAI TCCGGA 1 cut(s) 577
BseBI CCWGG 3 cut(s) 1579, 1623, 2236
BseDI CCNNGG 2 cut(s) 841, 2234
BseLI CCNNNNNNNGG 8 cut(s) 46, 78, 147, 399, 701, 1345, 2465, 2662
BseMII CTCAG 7 cut(s) 792, 1774, 1878, 2189, 2191, 2675, 3151
BseNI ACTGG 9 cut(s) 545, 550, 662, 1005, 1324, 1627, 2433, 2776, 3241
BseRI GAGGAG 4 cut(s) 68, 648, 971, 2619
BsgI GTGCAG 5 cut(s) 750, 1674, 2396, 2942, 3021
Bsh1236I CGCG 1 cut(s) 1141
Bsh1285I CGRYCG 1 cut(s) 726
BshFI GGCC 9 cut(s) 140, 627, 770, 1208, 1577, 1626, 2047, 2426, 2480
BshNI GGYRCC 1 cut(s) 2623
BsiEI CGRYCG 1 cut(s) 726
BsiHKAI GWGCWC 1 cut(s) 2135
BsiHKCI CYCGRG 2 cut(s) 2491, 2916
BsiSI CCGG 7 cut(s) 141, 578, 1073, 1461, 2079, 2272, 2814
BslFI GGGAC 2 cut(s) 164, 416
BslI CCNNNNNNNGG 8 cut(s) 46, 78, 147, 399, 701, 1345, 2465, 2662
BsmAI GTCTC 8 cut(s) 502, 787, 1422, 1692, 2581, 2906, 2957, 3321
BsmBI CGTCTC 3 cut(s) 1422, 2957, 3321
BsmFI GGGAC 2 cut(s) 164, 416
BsmI GAATGC 1 cut(s) 2651
BsnI GGCC 9 cut(s) 140, 627, 770, 1208, 1577, 1626, 2047, 2426, 2480
Bso31I GGTCTC 1 cut(s) 2581
BsoBI CYCGRG 2 cut(s) 2491, 2916
Bsp119I TTCGAA 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 2135
Bsp13I TCCGGA 1 cut(s) 577
Bsp1407I TGTACA 2 cut(s) 611, 1190
BspANI GGCC 9 cut(s) 140, 627, 770, 1208, 1577, 1626, 2047, 2426, 2480
BspCNI CTCAG 7 cut(s) 791, 1773, 1879, 2190, 2190, 2674, 3150
BspEI TCCGGA 1 cut(s) 577
BspFNI CGCG 1 cut(s) 1141
BspHI TCATGA 2 cut(s) 916, 1489
BspLI GGNNCC 3 cut(s) 2259, 2396, 2625
BspMAI CTGCAG 1 cut(s) 1960
BspMI ACCTGC 2 cut(s) 749, 1041
BspPI GGATC 5 cut(s) 712, 1051, 2252, 2265, 3020
BspT104I TTCGAA 1 cut(s) 222
BspT107I GGYRCC 1 cut(s) 2623
BspTI CTTAAG 1 cut(s) 440
BspTNI GGTCTC 1 cut(s) 2581
BsrBI CCGCTC 1 cut(s) 494
BsrFI RCCGGY 1 cut(s) 2271
BsrGI TGTACA 2 cut(s) 611, 1190
BsrI ACTGG 9 cut(s) 545, 550, 662, 1005, 1324, 1627, 2433, 2776, 3241
BssAI RCCGGY 1 cut(s) 2271
BssECI CCNNGG 2 cut(s) 841, 2234
BssNI GRCGYC 2 cut(s) 2496, 2624
BssSI CACGAG 1 cut(s) 2960
BssT1I CCWWGG 1 cut(s) 841
Bst2BI CACGAG 1 cut(s) 2960
Bst2UI CCWGG 3 cut(s) 1579, 1623, 2236
Bst6I CTCTTC 3 cut(s) 45, 790, 2744
BstACI GRCGYC 2 cut(s) 2496, 2624
BstAFI CTTAAG 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 737
BstAUI TGTACA 2 cut(s) 611, 1190
BstBI TTCGAA 1 cut(s) 222
BstC8I GCNNGC 5 cut(s) 418, 798, 1777, 2273, 2347
BstFNI CGCG 1 cut(s) 1141
BstH2I RGCGCY 2 cut(s) 2531, 2627
BstHHI GCGC 2 cut(s) 2530, 2626
BstMAI GTCTC 8 cut(s) 502, 787, 1422, 1692, 2581, 2906, 2957, 3321
BstMCI CGRYCG 1 cut(s) 726
BstMWI GCNNNNNNNGC 9 cut(s) 268, 500, 674, 737, 1911, 2072, 2438, 2452, 3158
BstNI CCWGG 3 cut(s) 1579, 1623, 2236
BstNSI RCATGY 1 cut(s) 612
BstSCI CCNGG 4 cut(s) 1071, 1577, 1621, 2234
BstSFI CTRYAG 1 cut(s) 1956
BstUI CGCG 1 cut(s) 1141
BstV2I GAAGAC 1 cut(s) 2462
BstX2I RGATCY 4 cut(s) 704, 1043, 2257, 3025
BstXI CCANNNNNNTGG 2 cut(s) 1021, 2689
BstYI RGATCY 4 cut(s) 704, 1043, 2257, 3025
Bsu36I CCTNAGG 1 cut(s) 2661
BsuRI GGCC 9 cut(s) 140, 627, 770, 1208, 1577, 1626, 2047, 2426, 2480
BtgZI GCGATG 1 cut(s) 3276
BtrI CACGTC 1 cut(s) 1251
BtsI GCAGTG 5 cut(s) 684, 1193, 2061, 2223, 2427
BveI ACCTGC 2 cut(s) 749, 1041
Cac8I GCNNGC 5 cut(s) 418, 798, 1777, 2273, 2347
CaiI CAGNNNCTG 2 cut(s) 737, 2383
CciI TCATGA 2 cut(s) 916, 1489
CfoI GCGC 2 cut(s) 2530, 2626
Cfr10I RCCGGY 1 cut(s) 2271
Cfr13I GGNCC 3 cut(s) 626, 1322, 2395
CseI GACGC 3 cut(s) 1892, 2485, 2974
CspCI CAANNNNNGTGG 2 cut(s) 2014, 2049
DinI GGCGCC 1 cut(s) 2625
DriI GACNNNNNGTC 1 cut(s) 1254
EaeI YGGCCR 3 cut(s) 768, 1624, 2478
Eam1104I CTCTTC 3 cut(s) 45, 790, 2744
Eam1105I GACNNNNNGTC 1 cut(s) 1254
EarI CTCTTC 3 cut(s) 45, 790, 2744
Ecl136II GAGCTC 1 cut(s) 2133
Eco130I CCWWGG 1 cut(s) 841
Eco147I AGGCCT 2 cut(s) 1577, 2047
Eco24I GRGCYC 1 cut(s) 2135
Eco31I GGTCTC 1 cut(s) 2581
Eco47I GGWCC 2 cut(s) 1322, 2395
Eco47III AGCGCT 1 cut(s) 2529
Eco53kI GAGCTC 1 cut(s) 2133
Eco57I CTGAAG 1 cut(s) 518
Eco81I CCTNAGG 1 cut(s) 2661
Eco88I CYCGRG 2 cut(s) 2491, 2916
EcoICRI GAGCTC 1 cut(s) 2133
EcoRI GAATTC 1 cut(s) 742
EcoRII CCWGG 3 cut(s) 1577, 1621, 2234
EcoT14I CCWWGG 1 cut(s) 841
EcoT22I ATGCAT 2 cut(s) 817, 2525
EcoT38I GRGCYC 1 cut(s) 2135
EgeI GGCGCC 1 cut(s) 2625
EheI GGCGCC 1 cut(s) 2625
ErhI CCWWGG 1 cut(s) 841
Esp3I CGTCTC 3 cut(s) 1422, 2957, 3321
FalI AAGNNNNNCTT 4 cut(s) 1923, 1955, 3015, 3047
FaqI GGGAC 2 cut(s) 164, 416
FauI CCCGC 3 cut(s) 499, 1134, 3213
FauNDI CATATG 1 cut(s) 2883
FbaI TGATCA 4 cut(s) 601, 898, 913, 2170
FblI GTMKAC 2 cut(s) 2147, 3362
FriOI GRGCYC 1 cut(s) 2135
FspBI CTAG 8 cut(s) 978, 1094, 1709, 2142, 2276, 2307, 2648, 2972
GlaI GCGC 2 cut(s) 2529, 2625
GsuI CTGGAG 3 cut(s) 645, 861, 2013
HaeII RGCGCY 2 cut(s) 2531, 2627
HaeIII GGCC 9 cut(s) 140, 627, 770, 1208, 1577, 1626, 2047, 2426, 2480
HapII CCGG 7 cut(s) 141, 578, 1073, 1461, 2079, 2272, 2814
HgaI GACGC 3 cut(s) 1892, 2485, 2974
HhaI GCGC 2 cut(s) 2530, 2626
Hin1I GRCGYC 2 cut(s) 2496, 2624
Hin6I GCGC 2 cut(s) 2528, 2624
HinP1I GCGC 2 cut(s) 2528, 2624
HincII GTYRAC 3 cut(s) 1609, 2244, 2500
HindII GTYRAC 3 cut(s) 1609, 2244, 2500
HindIII AAGCTT 1 cut(s) 479
HpaII CCGG 7 cut(s) 141, 578, 1073, 1461, 2079, 2272, 2814
HphI GGTGA 6 cut(s) 1061, 1835, 2165, 2746, 2747, 2921
Hpy99I CGWCG 1 cut(s) 3361
HpyAV CCTTC 7 cut(s) 268, 493, 705, 1478, 1724, 1925, 3293
HpyCH4IV ACGT 5 cut(s) 862, 974, 1085, 1250, 1311
HpyF10VI GCNNNNNNNGC 9 cut(s) 268, 500, 674, 737, 1911, 2072, 2438, 2452, 3158
HpySE526I ACGT 5 cut(s) 862, 974, 1085, 1250, 1311
Hsp92I GRCGYC 2 cut(s) 2496, 2624
HspAI GCGC 2 cut(s) 2528, 2624
KasI GGCGCC 1 cut(s) 2623
Kpn2I TCCGGA 1 cut(s) 577
KroI GCCGGC 1 cut(s) 2271
KroNI GCCGGC 1 cut(s) 2273
Ksp22I TGATCA 4 cut(s) 601, 898, 913, 2170
MaeI CTAG 8 cut(s) 978, 1094, 1709, 2142, 2276, 2307, 2648, 2972
MaeII ACGT 5 cut(s) 862, 974, 1085, 1250, 1311
MbiI CCGCTC 1 cut(s) 494
MfeI CAATTG 2 cut(s) 1212, 2113
MflI RGATCY 4 cut(s) 704, 1043, 2257, 3025
MhlI GDGCHC 1 cut(s) 2135
MlsI TGGCCA 3 cut(s) 770, 1626, 2480
MluNI TGGCCA 3 cut(s) 770, 1626, 2480
Mly113I GGCGCC 1 cut(s) 2624
MmeI TCCRAC 3 cut(s) 743, 995, 3031
Mox20I TGGCCA 3 cut(s) 770, 1626, 2480
Mph1103I ATGCAT 2 cut(s) 817, 2525
MroI TCCGGA 1 cut(s) 577
MroNI GCCGGC 1 cut(s) 2271
MroXI GAANNNNTTC 3 cut(s) 132, 1750, 2514
MscI TGGCCA 3 cut(s) 770, 1626, 2480
MseI TTAA 9 cut(s) 321, 441, 810, 1029, 1457, 1998, 2042, 2796, 3273
MslI CAYNNNNRTG 5 cut(s) 906, 1058, 1365, 2687, 3110
Msp20I TGGCCA 3 cut(s) 770, 1626, 2480
MspA1I CMGCKG 5 cut(s) 671, 734, 881, 2446, 3208
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 7 cut(s) 141, 578, 1073, 1461, 2079, 2272, 2814
MspR9I CCNGG 4 cut(s) 1073, 1579, 1623, 2236
MunI CAATTG 2 cut(s) 1212, 2113
Mva1269I GAATGC 1 cut(s) 2651
MvaI CCWGG 3 cut(s) 1579, 1623, 2236
MvnI CGCG 1 cut(s) 1141
MwoI GCNNNNNNNGC 9 cut(s) 268, 500, 674, 737, 1911, 2072, 2438, 2452, 3158
NaeI GCCGGC 1 cut(s) 2273
NarI GGCGCC 1 cut(s) 2624
NciI CCSGG 1 cut(s) 1073
NdeI CATATG 1 cut(s) 2883
NgoMIV GCCGGC 1 cut(s) 2271
NlaIV GGNNCC 3 cut(s) 2259, 2396, 2625
NmeAIII GCCGAG 1 cut(s) 2063
NmuCI GTSAC 7 cut(s) 996, 1051, 1246, 1417, 1948, 2753, 3095
NsiI ATGCAT 2 cut(s) 817, 2525
NspI RCATGY 1 cut(s) 612
NspV TTCGAA 1 cut(s) 222
PaeR7I CTCGAG 1 cut(s) 2916
PagI TCATGA 2 cut(s) 916, 1489
PceI AGGCCT 2 cut(s) 1577, 2047
PciI ACATGT 1 cut(s) 608
PctI GAATGC 1 cut(s) 2651
PdiI GCCGGC 1 cut(s) 2273
PdmI GAANNNNTTC 3 cut(s) 132, 1750, 2514
PfeI GAWTC 8 cut(s) 92, 99, 1300, 1920, 1968, 2057, 2696, 3293
PflMI CCANNNNNTGG 3 cut(s) 46, 1345, 2465
PluTI GGCGCC 1 cut(s) 2627
PscI ACATGT 1 cut(s) 608
Psp124BI GAGCTC 1 cut(s) 2135
Psp6I CCWGG 3 cut(s) 1577, 1621, 2234
PspGI CCWGG 3 cut(s) 1577, 1621, 2234
PspN4I GGNNCC 3 cut(s) 2259, 2396, 2625
PspPI GGNCC 3 cut(s) 626, 1322, 2395
PspXI VCTCGAGB 1 cut(s) 2916
PstI CTGCAG 1 cut(s) 1960
PstNI CAGNNNCTG 2 cut(s) 737, 2383
PsuI RGATCY 4 cut(s) 704, 1043, 2257, 3025
PvuII CAGCTG 2 cut(s) 734, 881
RseI CAYNNNNRTG 5 cut(s) 906, 1058, 1365, 2687, 3110
SacI GAGCTC 1 cut(s) 2135
SaqAI TTAA 9 cut(s) 321, 441, 810, 1029, 1457, 1998, 2042, 2796, 3273
Sau96I GGNCC 3 cut(s) 626, 1322, 2395
ScaI AGTACT 2 cut(s) 119, 167
ScrFI CCNGG 4 cut(s) 1073, 1579, 1623, 2236
SduI GDGCHC 1 cut(s) 2135
SfcI CTRYAG 1 cut(s) 1956
SfoI GGCGCC 1 cut(s) 2625
Sfr274I CTCGAG 1 cut(s) 2916
SfuI TTCGAA 1 cut(s) 222
SinI GGWCC 2 cut(s) 1322, 2395
SlaI CTCGAG 1 cut(s) 2916
SmiMI CAYNNNNRTG 5 cut(s) 906, 1058, 1365, 2687, 3110
SmlI CTYRAG 5 cut(s) 83, 440, 1915, 2916, 3171
SmoI CTYRAG 5 cut(s) 83, 440, 1915, 2916, 3171
SseBI AGGCCT 2 cut(s) 1577, 2047
SspDI GGCGCC 1 cut(s) 2623
SspMI CTAG 8 cut(s) 978, 1094, 1709, 2142, 2276, 2307, 2648, 2972
SstI GAGCTC 1 cut(s) 2135
StuI AGGCCT 2 cut(s) 1577, 2047
StyD4I CCNGG 4 cut(s) 1071, 1577, 1621, 2234
StyI CCWWGG 1 cut(s) 841
TaiI ACGT 5 cut(s) 865, 977, 1088, 1253, 1314
TaqII GACCGA 1 cut(s) 712
TatI WGTACW 4 cut(s) 117, 165, 611, 1190
TauI GCSGC 2 cut(s) 671, 3089
TfiI GAWTC 8 cut(s) 92, 99, 1300, 1920, 1968, 2057, 2696, 3293
Tru1I TTAA 9 cut(s) 321, 441, 810, 1029, 1457, 1998, 2042, 2796, 3273
Tru9I TTAA 9 cut(s) 321, 441, 810, 1029, 1457, 1998, 2042, 2796, 3273
TseFI GTSAC 7 cut(s) 996, 1051, 1246, 1417, 1948, 2753, 3095
Tsp45I GTSAC 7 cut(s) 996, 1051, 1246, 1417, 1948, 2753, 3095
TspGWI ACGGA 5 cut(s) 61, 1980, 2387, 3201, 3348
Van91I CCANNNNNTGG 3 cut(s) 46, 1345, 2465
Vha464I CTTAAG 1 cut(s) 440
VpaK11BI GGWCC 2 cut(s) 1322, 2395
XapI RAATTY 6 cut(s) 132, 367, 742, 2250, 2335, 2940
XbaI TCTAGA 1 cut(s) 977
XceI RCATGY 1 cut(s) 612
XcmI CCANNNNNNNNNTGG 4 cut(s) 1170, 2414, 3205, 3248
XhoI CTCGAG 1 cut(s) 2916
XmiI GTMKAC 2 cut(s) 2147, 3362
XmnI GAANNNNTTC 3 cut(s) 132, 1750, 2514
XspI CTAG 8 cut(s) 978, 1094, 1709, 2142, 2276, 2307, 2648, 2972
ZrmI AGTACT 2 cut(s) 119, 167
Zsp2I ATGCAT 2 cut(s) 817, 2525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.