Rh3DG077800

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005953
Physical Location & Seq
Forward (+)
6048793 .. 6055356
6564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG077800.1

Sequence Viewer

Length: 2655 bp
ATGAAATTACATTTGCAATGTCGTAGTGTACGTAAGATTCTATTTTTTGATTTAGCAGTTCCAAGCCTAAAGCAGCCAAGAACAGCTCTGGAAACTTCTGGTGGGGTTGATCCTTCTAGTTTTACTGCTATTGACCGGATGGCGAAAGAACTGCAACATGTTCGATCGAACTTGGAGGGTACCTTTCAAACTTCTAGACTGCTTCAAACTTCCTGGTTGGTGGAGAACCTACCGAGAAATGTGGCTTATAGCATGCGTCAAGGATCTACAGTCACAAGCATGGATTTTCATCCTTATCACCATGCATTGCTCCTAGTTGGTGCTAGCAATGGTGAAATTAGTCTTTGGGAAGTTGGAACTCGGCAGAGGGTGGCTTCGCAGCCATTCAGAATATGGAATACGGCAGCACGTTCATCAGAACTTCAGAGCACACTTGCCAATGAAGACACATCTATATCTGTGAGCCGTGTTACATGGAGTCCAGATGGGAACTTTTTTGGAGTTGCATTTACTAAAAATTTGATTCACTTGTATGCTTTTTCTGGAATTAGTGATCTATGCCGGTATTTGGAGGTTGACGCCCATGATGGTGGTGTGAATGACTTGGCTTTTGATTATCGAAACAACGAACTGTGTGTTATCACATGTGGAGATGACAAGCTAATAAAGGTATGGGATATAGCAGGACGAAACTTATATAACTTTGATGGTCATGAAGCACCGGTCTATTCAGTGTGTCCTTATCATAACAAGGATGTTCAGTACATATATTCAACTTCTACTGATGGGAAGATAAAGACCTGGATGTACGACAATGGGGGTTCTAGATCTGGCTGTGATGCTCCTGGCAACTGGTGTACTACATTGCTTTTCAGTCCGGATGGTAACCGAATGTTCTCTTGTGGAACAAATAAAGAGGGAGACACGTTTCTAGTTCAATGGAGCGAAAATGAAGGACTAATTAAAGAAGGAGCAAAAAAGAGAATTTATTCTGGGTTTGGTAAGAAATCTGAAGGGGTTGTGCATTTTGACATAACAAAAAAGCATTTTTTGGCTGTGGGGGAAGATAGCCAGATCAAGTATTGGGATATGGACCATGATGATGTTCTGACTAGCACAGACGCCCAGGGTGAACTGCCGAGTCGTCCTCGCTTGAGATTCAACAAGGAAGGTAGTCTTCTTGCTGTCACAACGGCACATGAGGGATTCATGATACTTGCAAATGGAATCGGACGCCTCAGAGTTCATGGAGTCGCTGCAACTTATCGTGACAGGTCGAAAGATCCTACTGAATCCACTGCAAACAAGGCATCTGGCTCATCTTCGGTTGCAAATGTCAGTGAGGCCAATAGGAAATCTGTTGCAAATGCCAGTGAGGCCAATAGGAAATCTGACAGGGGCTCTCCTGCTAGGTCTACAACATCTCAGAATGGAGCTAATAATGGAGTTAATACCAAGAGTAGAAGTTCCAAGAAACGGAAAACTGTGGAGGAGGTAATCAATAAATCCAAACCTTGGCAGTTGACTGACATTCGGGATCCTGTAAGGTGTCGTTTAGGTGTTATGCCTGAAACAGATAGTTCCTGCAAGGTTGTTCGACTCCTATATACAAGTAACGGTACTGCCATCTTGGCACTTGGGTCAAATGGTGTACAGAGGCTGTGGAGGTGGCCCCGAACTAGAGGTCGAATTTCAAAGGCCAGCGCCAGTGTTGTTCCGCAGCTGGTGAAAGGGGACAATGGTCCTGTAATGGCCAATGATGTCTCAGGTGTCAACCTTGAAGAGGTGGTTCCCTGCATAGCGCTCTCAAAGAATGACGCGTATGTAATGTCAGCTTATGGTGGAAGGATCAGATTATTCAACATTTATAATTTGGCGGCAATGATAACTTTTGGGGAACTTCCCCCTGCACCAACTGAAATTGCATTCAATCCTCGGGATAATAATATCATGGCCATTGGAATGCAAGATTGCACCATACAGATTTTCTTTTGTAGACTTAATGAGGTAAGAGCAATCTTGAAGTTTCACAAGAAGTGGATAACTGGTTTAGTTTTCTCGTTCTTTCTTGGTATACTCGTTTCAGCTAGTGCCGATGGTCAAATATGTGTGTGGAGCATTGATCAATGGGAGAAGAAACAATCTGTTGCCTTATGTTTAGGTTCTGAAAAGACACCTGAGGGTGCCACTCAATTGCAGTTCCACTGCAACCAAGTCCGCTTACTGGTGTTTCATGAAACACAACTGGCAGTTTATGATGCCTCAAAGATGAAGCTCCTCCGGCAGTGGCTTCCACACGGTATGCTTTCTGCTCCCATACGTCATGCAGCATACTCCTTTGACAGTCGACTGGTTTATACCGCCTTCAGTGATGGTAACATCGGAGTATTTGATGCCAATAGCCTAAGACTGAGATGTCGTATTGCTCCATCAGCATACTTACCGGAAGCAGTCTTGAAGAGGACCGATGAATTGTATCCTCTTGTCATTGCAGGAAATCCCCATGTACAAAACCAGTTTGCTGTTGGGTTGTCTGATGGTACTGTTAAGGTCATTGAGCCATTATTTGGTGACTGGGGGTCCTCCCCACCTCCTGATGATGAAGCTTCATCAAGTGCCACCAACAACCACTCGTCCAGACAAGTACAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

884

Amino Acids

97.8

Weight (kDa)

8.71

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 89 - 305 7.3e-08 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 129 - 306 3.3e-16 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 130 - 304 2.9e-07 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 144 - 273 4.1e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 151 - 369 8.1e-20 WDR5 beta-propeller domain
WD40_Prp19 PF24814 194 - 305 1.1e-07 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 208 - 273 1.4e-07 WDHD1 first WD40 domain
EIF3I PF24805 212 - 375 2.1e-06 EIF3I
Beta-prop_THOC3 PF25174 215 - 304 4.9e-08 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 586 - 801 1.6e-13 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 591 - 715 1.7e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 595 - 714 4.5e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 597 - 710 1.4e-09 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 597 - 807 4e-12 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 598 - 797 1.9e-07 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 599 - 808 7e-09 THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 616 - 711 2.8e-06 WDR90/POC16, second beta-propeller
WD40_Prp19 PF24814 639 - 802 4.8e-09 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 677 - 802 1.5e-06 WDR36/Utp21 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1869
AasI GACNNNNNNGTC 1 cut(s) 2415
Acc65I GGTACC 1 cut(s) 179
AccB1I GGYRCC 2 cut(s) 179, 2183
AccB7I CCANNNNNTGG 2 cut(s) 1515, 2567
AccI GTMKAC 4 cut(s) 1415, 1996, 2074, 2347
AccII CGCG 1 cut(s) 1820
AccIII TCCGGA 1 cut(s) 877
AciI CCGC 4 cut(s) 1718, 1877, 2218, 2361
AclWI GGATC 6 cut(s) 104, 271, 1277, 1532, 1545, 1856
AcoI YGGCCR 2 cut(s) 1752, 1953
AcsI RAATTY 3 cut(s) 517, 984, 1689
AcuI CTGAAG 3 cut(s) 407, 1032, 2350
AcyI GRCGYC 3 cut(s) 579, 1122, 1234
AfeI AGCGCT 1 cut(s) 1803
AfiI CCNNNNNNNGG 6 cut(s) 568, 1476, 1515, 1783, 2224, 2567
AflIII ACRYGT 4 cut(s) 157, 644, 924, 1818
AgeI ACCGGT 1 cut(s) 721
AhdI GACNNNNNGTC 1 cut(s) 2578
AjnI CCWGG 4 cut(s) 212, 800, 844, 1125
AluBI AGCT 8 cut(s) 86, 661, 1436, 1723, 1835, 2087, 2275, 2606
AluI AGCT 8 cut(s) 86, 661, 1436, 1723, 1835, 2087, 2275, 2606
Alw21I GWGCWC 1 cut(s) 431
Alw26I GTCTC 2 cut(s) 915, 1768
AlwI GGATC 6 cut(s) 104, 271, 1277, 1532, 1545, 1856
AlwNI CAGNNNCTG 1 cut(s) 1660
Ama87I CYCGRG 1 cut(s) 1935
Aor13HI TCCGGA 1 cut(s) 877
Aor51HI AGCGCT 1 cut(s) 1803
AoxI GGCC 6 cut(s) 1344, 1377, 1670, 1698, 1752, 1953
ApeKI GCWGC 6 cut(s) 73, 379, 404, 1256, 1720, 2327
ApoI RAATTY 3 cut(s) 517, 984, 1689
ArsI GACNNNNNNTTYG 4 cut(s) 593, 625, 1669, 1701
AsiGI ACCGGT 1 cut(s) 721
Asp700I GAANNNNTTC 1 cut(s) 988
Asp718I GGTACC 1 cut(s) 179
AspLEI GCGC 2 cut(s) 1706, 1804
AspS9I GGNCC 5 cut(s) 1093, 1671, 1742, 2463, 2580
AsuHPI GGTGA 5 cut(s) 290, 344, 1142, 1738, 2582
AsuNHI GCTAGC 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 1935
AvaII GGWCC 4 cut(s) 1093, 1742, 2463, 2580
AxyI CCTNAGG 1 cut(s) 2178
BalI TGGCCA 2 cut(s) 1754, 1955
BamHI GGATCC 1 cut(s) 1537
BanI GGYRCC 2 cut(s) 179, 2183
BanII GRGCYC 1 cut(s) 1403
BbsI GAAGAC 2 cut(s) 450, 1169
Bbv12I GWGCWC 1 cut(s) 431
BbvI GCAGC 6 cut(s) 85, 391, 416, 1243, 1732, 2339
BceAI ACGGC 3 cut(s) 417, 450, 1209
BcgI CGANNNNNNTGC 4 cut(s) 133, 143, 167, 177
BciT130I CCWGG 4 cut(s) 214, 802, 846, 1127
BciVI GTATCC 1 cut(s) 2487
BclI TGATCA 1 cut(s) 2122
BcoDI GTCTC 2 cut(s) 915, 1768
BfmI CTRYAG 1 cut(s) 267
BfoI RGCGCY 2 cut(s) 1707, 1805
BfuI GTATCC 1 cut(s) 2487
BglI GCCNNNNNGGC 2 cut(s) 1376, 1631
BglII AGATCT 1 cut(s) 827
BisI GCNGC 7 cut(s) 74, 380, 405, 1257, 1721, 1878, 2328
BlsI GCNGC 7 cut(s) 75, 381, 406, 1258, 1722, 1879, 2329
Bme1390I CCNGG 4 cut(s) 214, 802, 846, 1127
Bme18I GGWCC 4 cut(s) 1093, 1742, 2463, 2580
BmeRI GACNNNNNGTC 1 cut(s) 2578
BmeT110I CYCGRG 1 cut(s) 1935
BmgT120I GGNCC 5 cut(s) 1093, 1671, 1742, 2463, 2580
BmiI GGNNCC 6 cut(s) 181, 1539, 1673, 1791, 2185, 2581
BmrFI CCNGG 4 cut(s) 214, 802, 846, 1127
BmrI ACTGGG 1 cut(s) 2584
BmsI GCATC 4 cut(s) 829, 1319, 2248, 2383
BmtI GCTAGC 1 cut(s) 327
BmuI ACTGGG 1 cut(s) 2584
BoxI GACNNNNGTC 1 cut(s) 1740
BpiI GAAGAC 2 cut(s) 450, 1169
BplI GAGNNNNNCTC 2 cut(s) 1132, 1164
BpuEI CTTGAG 1 cut(s) 1174
BsaAI YACGTR 1 cut(s) 32
BsaBI GATNNNNATC 1 cut(s) 288
BsaHI GRCGYC 3 cut(s) 579, 1122, 1234
BsaJI CCNNGG 4 cut(s) 1125, 1126, 1514, 1934
BsaWI WCCGGW 4 cut(s) 135, 721, 877, 2443
Bsc4I CCNNNNNNNGG 6 cut(s) 568, 1476, 1515, 1783, 2224, 2567
Bse118I RCCGGY 2 cut(s) 561, 721
Bse1I ACTGG 9 cut(s) 857, 1371, 1707, 2050, 2229, 2250, 2355, 2515, 2579
Bse21I CCTNAGG 1 cut(s) 2178
Bse3DI GCAATG 6 cut(s) 23, 305, 334, 863, 1887, 2487
Bse8I GATNNNNATC 1 cut(s) 288
BseAI TCCGGA 1 cut(s) 877
BseBI CCWGG 4 cut(s) 214, 802, 846, 1127
BseDI CCNNGG 4 cut(s) 1125, 1126, 1514, 1934
BseGI GGATG 5 cut(s) 144, 289, 760, 810, 886
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 6 cut(s) 568, 1476, 1515, 1783, 2224, 2567
BseMI GCAATG 6 cut(s) 23, 305, 334, 863, 1887, 2487
BseMII CTCAG 5 cut(s) 1252, 1439, 1779, 2169, 2402
BseNI ACTGG 9 cut(s) 857, 1371, 1707, 2050, 2229, 2250, 2355, 2515, 2579
BseRI GAGGAG 2 cut(s) 1505, 2267
BseXI GCAGC 6 cut(s) 85, 391, 416, 1243, 1732, 2339
BsgI GTGCAG 1 cut(s) 1893
Bsh1236I CGCG 1 cut(s) 1820
Bsh1285I CGRYCG 1 cut(s) 167
BshFI GGCC 6 cut(s) 1346, 1379, 1672, 1700, 1754, 1955
BshNI GGYRCC 2 cut(s) 179, 2183
BshTI ACCGGT 1 cut(s) 721
BsiEI CGRYCG 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 431
BsiHKCI CYCGRG 1 cut(s) 1935
BsiSI CCGG 6 cut(s) 136, 562, 722, 878, 2281, 2444
BslFI GGGAC 1 cut(s) 1748
BslI CCNNNNNNNGG 6 cut(s) 568, 1476, 1515, 1783, 2224, 2567
BsmAI GTCTC 2 cut(s) 915, 1768
BsmFI GGGAC 1 cut(s) 1748
BsmI GAATGC 2 cut(s) 1925, 1968
BsnI GGCC 6 cut(s) 1346, 1379, 1672, 1700, 1754, 1955
BsoBI CYCGRG 1 cut(s) 1935
Bsp1286I GDGCHC 2 cut(s) 431, 1403
Bsp13I TCCGGA 1 cut(s) 877
Bsp1407I TGTACA 2 cut(s) 1651, 2506
BspACI CCGC 4 cut(s) 1718, 1877, 2218, 2361
BspANI GGCC 6 cut(s) 1346, 1379, 1672, 1700, 1754, 1955
BspCNI CTCAG 5 cut(s) 1251, 1438, 1778, 2170, 2403
BspEI TCCGGA 1 cut(s) 877
BspFNI CGCG 1 cut(s) 1820
BspHI TCATGA 3 cut(s) 712, 1209, 2233
BspLI GGNNCC 6 cut(s) 181, 1539, 1673, 1791, 2185, 2581
BspOI GCTAGC 1 cut(s) 327
BspPI GGATC 6 cut(s) 104, 271, 1277, 1532, 1545, 1856
BspT107I GGYRCC 2 cut(s) 179, 2183
BsrDI GCAATG 6 cut(s) 23, 305, 334, 863, 1887, 2487
BsrFI RCCGGY 2 cut(s) 561, 721
BsrGI TGTACA 2 cut(s) 1651, 2506
BsrI ACTGG 9 cut(s) 857, 1371, 1707, 2050, 2229, 2250, 2355, 2515, 2579
BssAI RCCGGY 2 cut(s) 561, 721
BssECI CCNNGG 4 cut(s) 1125, 1126, 1514, 1934
BssNAI GTATAC 1 cut(s) 2075
BssNI GRCGYC 3 cut(s) 579, 1122, 1234
BssT1I CCWWGG 1 cut(s) 1514
Bst1107I GTATAC 1 cut(s) 2075
Bst2UI CCWGG 4 cut(s) 214, 802, 846, 1127
Bst4CI ACNGT 7 cut(s) 271, 633, 1486, 1619, 2300, 2345, 2545
Bst6I CTCTTC 2 cut(s) 1776, 2453
BstACI GRCGYC 3 cut(s) 579, 1122, 1234
BstAUI TGTACA 2 cut(s) 1651, 2506
BstBAI YACGTR 1 cut(s) 32
BstC8I GCNNGC 3 cut(s) 254, 325, 1702
BstDEI CTNAG 6 cut(s) 1238, 1425, 1765, 2178, 2405, 2411
BstEII GGTNACC 1 cut(s) 884
BstENI CCTNNNNNAGG 1 cut(s) 1781
BstF5I GGATG 5 cut(s) 144, 289, 760, 810, 886
BstFNI CGCG 1 cut(s) 1820
BstH2I RGCGCY 2 cut(s) 1707, 1805
BstHHI GCGC 2 cut(s) 1706, 1804
BstMAI GTCTC 2 cut(s) 915, 1768
BstMCI CGRYCG 1 cut(s) 167
BstMWI GCNNNNNNNGC 5 cut(s) 1307, 1376, 1631, 2281, 2432
BstNI CCWGG 4 cut(s) 214, 802, 846, 1127
BstNSI RCATGY 3 cut(s) 161, 256, 648
BstPAI GACNNNNGTC 1 cut(s) 1740
BstPI GGTNACC 1 cut(s) 884
BstSCI CCNGG 4 cut(s) 212, 800, 844, 1125
BstSFI CTRYAG 1 cut(s) 267
BstSNI TACGTA 1 cut(s) 32
BstUI CGCG 1 cut(s) 1820
BstV1I GCAGC 6 cut(s) 85, 391, 416, 1243, 1732, 2339
BstV2I GAAGAC 2 cut(s) 450, 1169
BstX2I RGATCY 4 cut(s) 263, 827, 1282, 1537
BstXI CCANNNNNNTGG 1 cut(s) 590
BstYI RGATCY 4 cut(s) 263, 827, 1282, 1537
BstZ17I GTATAC 1 cut(s) 2075
Bsu36I CCTNAGG 1 cut(s) 2178
BsuI GTATCC 1 cut(s) 2487
BsuRI GGCC 6 cut(s) 1346, 1379, 1672, 1700, 1754, 1955
BtsCI GGATG 5 cut(s) 144, 289, 760, 810, 886
BtsI GCAGTG 3 cut(s) 1296, 2203, 2291
BtsIMutI CAGTG 8 cut(s) 738, 1296, 1345, 1378, 1714, 2203, 2291, 2374
Cac8I GCNNGC 3 cut(s) 254, 325, 1702
CaiI CAGNNNCTG 1 cut(s) 1660
CciI TCATGA 3 cut(s) 712, 1209, 2233
CfoI GCGC 2 cut(s) 1706, 1804
Cfr10I RCCGGY 2 cut(s) 561, 721
Cfr13I GGNCC 5 cut(s) 1093, 1671, 1742, 2463, 2580
CseI GACGC 5 cut(s) 245, 587, 1130, 1242, 1826
CspAI ACCGGT 1 cut(s) 721
DdeI CTNAG 6 cut(s) 1238, 1425, 1765, 2178, 2405, 2411
DrdI GACNNNNNNGTC 1 cut(s) 2415
DriI GACNNNNNGTC 1 cut(s) 2578
DseDI GACNNNNNNGTC 1 cut(s) 2415
EaeI YGGCCR 2 cut(s) 1752, 1953
Eam1104I CTCTTC 2 cut(s) 1776, 2453
Eam1105I GACNNNNNGTC 1 cut(s) 2578
EarI CTCTTC 2 cut(s) 1776, 2453
Eco105I TACGTA 1 cut(s) 32
Eco130I CCWWGG 1 cut(s) 1514
Eco24I GRGCYC 1 cut(s) 1403
Eco47I GGWCC 4 cut(s) 1093, 1742, 2463, 2580
Eco47III AGCGCT 1 cut(s) 1803
Eco57I CTGAAG 3 cut(s) 407, 1032, 2350
Eco81I CCTNAGG 1 cut(s) 2178
Eco88I CYCGRG 1 cut(s) 1935
Eco91I GGTNACC 1 cut(s) 884
EcoNI CCTNNNNNAGG 1 cut(s) 1781
EcoO109I RGGNCCY 1 cut(s) 2580
EcoO65I GGTNACC 1 cut(s) 884
EcoRII CCWGG 4 cut(s) 212, 800, 844, 1125
EcoT14I CCWWGG 1 cut(s) 1514
EcoT22I ATGCAT 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 1403
ErhI CCWWGG 1 cut(s) 1514
FalI AAGNNNNNCTT 2 cut(s) 1161, 1193
FaqI GGGAC 1 cut(s) 1748
FbaI TGATCA 1 cut(s) 2122
FblI GTMKAC 4 cut(s) 1415, 1996, 2074, 2347
Fnu4HI GCNGC 7 cut(s) 74, 380, 405, 1257, 1721, 1878, 2328
FokI GGATG 5 cut(s) 151, 276, 767, 817, 893
FriOI GRGCYC 1 cut(s) 1403
Fsp4HI GCNGC 7 cut(s) 74, 380, 405, 1257, 1721, 1878, 2328
GlaI GCGC 2 cut(s) 1705, 1803
GluI GCNGC 7 cut(s) 74, 380, 405, 1257, 1721, 1878, 2328
HaeII RGCGCY 2 cut(s) 1707, 1805
HaeIII GGCC 6 cut(s) 1346, 1379, 1672, 1700, 1754, 1955
HapII CCGG 6 cut(s) 136, 562, 722, 878, 2281, 2444
HgaI GACGC 5 cut(s) 245, 587, 1130, 1242, 1826
HhaI GCGC 2 cut(s) 1706, 1804
Hin1I GRCGYC 3 cut(s) 579, 1122, 1234
Hin6I GCGC 2 cut(s) 1704, 1802
HinP1I GCGC 2 cut(s) 1704, 1802
HincII GTYRAC 4 cut(s) 577, 1524, 1774, 2348
HindII GTYRAC 4 cut(s) 577, 1524, 1774, 2348
HindIII AAGCTT 1 cut(s) 2604
HpaII CCGG 6 cut(s) 136, 562, 722, 878, 2281, 2444
HphI GGTGA 5 cut(s) 290, 344, 1142, 1738, 2582
HpyAV CCTTC 7 cut(s) 123, 947, 962, 1007, 1163, 1839, 2374
HpyCH4III ACNGT 7 cut(s) 271, 633, 1486, 1619, 2300, 2345, 2545
HpyCH4IV ACGT 4 cut(s) 31, 409, 926, 2320
HpyF10VI GCNNNNNNNGC 5 cut(s) 1307, 1376, 1631, 2281, 2432
HpyF3I CTNAG 6 cut(s) 1238, 1425, 1765, 2178, 2405, 2411
HpySE526I ACGT 4 cut(s) 31, 409, 926, 2320
Hsp92I GRCGYC 3 cut(s) 579, 1122, 1234
HspAI GCGC 2 cut(s) 1704, 1802
Kpn2I TCCGGA 1 cut(s) 877
KpnI GGTACC 1 cut(s) 183
Ksp22I TGATCA 1 cut(s) 2122
LmnI GCTCC 9 cut(s) 315, 847, 942, 971, 1433, 2115, 2280, 2317, 2431
Lsp1109I GCAGC 6 cut(s) 85, 391, 416, 1243, 1732, 2339
LweI GCATC 4 cut(s) 829, 1319, 2248, 2383
MaeII ACGT 4 cut(s) 31, 409, 926, 2320
MaeIII GTNAC 8 cut(s) 271, 469, 884, 1186, 1268, 1613, 2375, 2570
MboII GAAGA 8 cut(s) 455, 802, 1076, 1169, 1314, 1793, 2146, 2470
MfeI CAATTG 1 cut(s) 2192
MflI RGATCY 4 cut(s) 263, 827, 1282, 1537
MhlI GDGCHC 2 cut(s) 431, 1403
MlsI TGGCCA 2 cut(s) 1754, 1955
MluI ACGCGT 1 cut(s) 1818
MluNI TGGCCA 2 cut(s) 1754, 1955
MlyI GAGTC 4 cut(s) 487, 1150, 1260, 1593
MmeI TCCRAC 1 cut(s) 334
Mox20I TGGCCA 2 cut(s) 1754, 1955
Mph1103I ATGCAT 1 cut(s) 307
MroI TCCGGA 1 cut(s) 877
MroXI GAANNNNTTC 1 cut(s) 988
MscI TGGCCA 2 cut(s) 1754, 1955
MseI TTAA 4 cut(s) 963, 1449, 2001, 2547
MslI CAYNNNNRTG 5 cut(s) 278, 531, 588, 1101, 1961
Msp20I TGGCCA 2 cut(s) 1754, 1955
MspA1I CMGCKG 1 cut(s) 1723
MspI CCGG 6 cut(s) 136, 562, 722, 878, 2281, 2444
MspR9I CCNGG 4 cut(s) 214, 802, 846, 1127
MunI CAATTG 1 cut(s) 2192
Mva1269I GAATGC 2 cut(s) 1925, 1968
MvaI CCWGG 4 cut(s) 214, 802, 846, 1127
MvnI CGCG 1 cut(s) 1820
MwoI GCNNNNNNNGC 5 cut(s) 1307, 1376, 1631, 2281, 2432
NheI GCTAGC 1 cut(s) 323
NlaIV GGNNCC 6 cut(s) 181, 1539, 1673, 1791, 2185, 2581
NmeAIII GCCGAG 2 cut(s) 340, 1164
NmuCI GTSAC 4 cut(s) 271, 1186, 1268, 2570
NsiI ATGCAT 1 cut(s) 307
NspI RCATGY 3 cut(s) 161, 256, 648
PaeI GCATGC 1 cut(s) 256
PagI TCATGA 3 cut(s) 712, 1209, 2233
PasI CCCWGGG 1 cut(s) 1126
PciI ACATGT 2 cut(s) 157, 644
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PctI GAATGC 2 cut(s) 1925, 1968
PdmI GAANNNNTTC 1 cut(s) 988
PfeI GAWTC 6 cut(s) 37, 523, 1158, 1206, 1227, 1292
PflMI CCANNNNNTGG 2 cut(s) 1515, 2567
PinAI ACCGGT 1 cut(s) 721
PkrI GCNGC 7 cut(s) 75, 381, 406, 1258, 1722, 1879, 2329
Ple19I CGATCG 1 cut(s) 167
PleI GAGTC 4 cut(s) 486, 1149, 1259, 1593
PpsI GAGTC 4 cut(s) 486, 1149, 1259, 1593
Ppu21I YACGTR 1 cut(s) 32
PpuMI RGGWCCY 1 cut(s) 2580
PscI ACATGT 2 cut(s) 157, 644
PshAI GACNNNNGTC 1 cut(s) 1740
PsiI TTATAA 1 cut(s) 1869
Psp5II RGGWCCY 1 cut(s) 2580
Psp6I CCWGG 4 cut(s) 212, 800, 844, 1125
PspEI GGTNACC 1 cut(s) 884
PspGI CCWGG 4 cut(s) 212, 800, 844, 1125
PspN4I GGNNCC 6 cut(s) 181, 1539, 1673, 1791, 2185, 2581
PspPI GGNCC 5 cut(s) 1093, 1671, 1742, 2463, 2580
PspPPI RGGWCCY 1 cut(s) 2580
PstNI CAGNNNCTG 1 cut(s) 1660
PsuI RGATCY 4 cut(s) 263, 827, 1282, 1537
PvuI CGATCG 1 cut(s) 167
PvuII CAGCTG 1 cut(s) 1723
RseI CAYNNNNRTG 5 cut(s) 278, 531, 588, 1101, 1961
SalI GTCGAC 1 cut(s) 2346
SaqAI TTAA 4 cut(s) 963, 1449, 2001, 2547
SatI GCNGC 7 cut(s) 74, 380, 405, 1257, 1721, 1878, 2328
Sau96I GGNCC 5 cut(s) 1093, 1671, 1742, 2463, 2580
SchI GAGTC 4 cut(s) 487, 1150, 1260, 1593
ScrFI CCNGG 4 cut(s) 214, 802, 846, 1127
SduI GDGCHC 2 cut(s) 431, 1403
SfaNI GCATC 4 cut(s) 829, 1319, 2248, 2383
SfcI CTRYAG 1 cut(s) 267
SinI GGWCC 4 cut(s) 1093, 1742, 2463, 2580
SmiMI CAYNNNNRTG 5 cut(s) 278, 531, 588, 1101, 1961
SmlI CTYRAG 1 cut(s) 1153
SmoI CTYRAG 1 cut(s) 1153
SnaBI TACGTA 1 cut(s) 32
SphI GCATGC 1 cut(s) 256
SsiI CCGC 4 cut(s) 1718, 1877, 2218, 2361
StyD4I CCNGG 4 cut(s) 212, 800, 844, 1125
StyI CCWWGG 1 cut(s) 1514
TaaI ACNGT 7 cut(s) 271, 633, 1486, 1619, 2300, 2345, 2545
TaiI ACGT 4 cut(s) 34, 412, 929, 2323
TaqI TCGA 7 cut(s) 163, 167, 619, 1277, 1597, 1687, 2347
TaqII GACCGA 1 cut(s) 2480
TatI WGTACW 5 cut(s) 762, 857, 1651, 2506, 2644
TauI GCSGC 1 cut(s) 1880
TfiI GAWTC 6 cut(s) 37, 523, 1158, 1206, 1227, 1292
Tru1I TTAA 4 cut(s) 963, 1449, 2001, 2547
Tru9I TTAA 4 cut(s) 963, 1449, 2001, 2547
TscAI CASTG 8 cut(s) 738, 1303, 1345, 1378, 1714, 2210, 2291, 2374
TseFI GTSAC 4 cut(s) 271, 1186, 1268, 2570
TseI GCWGC 6 cut(s) 73, 379, 404, 1256, 1720, 2327
Tsp45I GTSAC 4 cut(s) 271, 1186, 1268, 2570
TspGWI ACGGA 1 cut(s) 1492
TspRI CASTG 8 cut(s) 738, 1303, 1345, 1378, 1714, 2210, 2291, 2374
Van91I CCANNNNNTGG 2 cut(s) 1515, 2567
VpaK11BI GGWCC 4 cut(s) 1093, 1742, 2463, 2580
XagI CCTNNNNNAGG 1 cut(s) 1781
XapI RAATTY 3 cut(s) 517, 984, 1689
XbaI TCTAGA 2 cut(s) 194, 824
XceI RCATGY 3 cut(s) 161, 256, 648
XcmI CCANNNNNNNNNTGG 2 cut(s) 390, 2522
XmiI GTMKAC 4 cut(s) 1415, 1996, 2074, 2347
XmnI GAANNNNTTC 1 cut(s) 988
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.