Rorug03G0013600

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005953
Physical Location & Seq
Reverse (-)
1052068 .. 1054145
2078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0013600.1

Sequence Viewer

Length: 2001 bp
ATGATTGCTCAGTTTTCCGACTCCGTTGTGTTCAGATTCTTCAGCACACTCTTCGTCGAAGAAGAGATTTCTCCGATGAGGAATTCCGGTTTCTCTAGCACTGTACCGGAAAGAGCTGAATCTGAGATTCGGAAAAGTTACGAAGAAGATGATGATTCAGAATTCTTTGATGTTGGTGATATAGAGATCTCAGAGCCAAATTTCGTGTTTAAATTTGAGTACCAGACTGGGGAAATATTAAAAACACTGGAAAAAGCGGATGTAATTGGTGATTATGTAGCTTTTCAGGCCCAAAAGAATACTAGTAGTAGTTGCTTCATTGAAGAAGCAGAAAATGGGTCTGCTCAGAAATCAAACTGTGGTTCTATAGAAGCAACTGATGTTGATGAAGGAAAAAGTGCTCAGGGTTTTACAGAGGGTTTTGACCAATTGGTTAGACAAGTACAAGTTCATGAGGAAAAAGGTACTGATCAGGGTTTTAGAGATGGTGCATTTGAGGAAGAGGAGGTAGAGAAGAAATCAGTGATTGAAGAAGATTCTGTTTCGGACGACGAAGAAGGATCCGAGAGTCAGGAAGAAGTAGATGTTAATAATGAAGAAATGTCTCATTCAGAAGAGGAAAGTGATAGTACTGGTGATGAGAAACTGGCTACTAGTCATGAAGATGAGTTGGTTTCGGAAGAGAACTTTGTTAGTTCGGAATCTGATTCTGATTTGATTTGCTCAAGTCTAGATGATGATGAAGTAGGTGCTTTGGAAGACATTGGATTGCACGAGCCTGATGGTTTTGATGAAGAAGATGATGATATAATGGAACAAGTTAGAGAACTAGAAGAGTTGTGTACGACTGATGATCAAAACTCGAAAGGGTCCGAACCTAAAGGAGAAGAAAAGCTAGTGGATGATTATAATGGTTCCGAGGATTCAGACACATTGGACGTTTTGTGGGAACACCAAGAGTTGATAGACCAGTTGAAAATGGAGCTCAAGATAGTCAGAGCTACAACTGGTCTGCCTACAATTTTTGAAGAATCCGAGTCTCTGGATATGGATGACTTGAAGCCACTCAAGATTGATGAAAAATACCAACACGGAGATAGAATGAGGGTGCTTCACAAGTTCTACAAGAGTTACAGGACTCGAATGCGCAAGTTGGATATTTTGAATTACCAGAAGTTATATGCAATAGGTGTTCTGCAGTCTAAGGATCCACTTAGATCGTTTTCGAGCTACAAATCCTCAACTCCGGCAATCAAATCCTTTCTTTCCAACTGGAGGCTATGGCAGTTGTGCCTTTCATGGGAGTTCCTTCAATGGCAATATGAGAAGGCCTTGGAGCTATTGGAATCTGCCCCTAATCAAATACATTCATACAATGAAGTTGCAGAGGAATTGCAACATTTTCAAGTGCTCCTGCAAAGATTTATAGAGGATGAACATTTTCAAGGGGCAAGGGTCGAAAACTATGTTAAAAATCGAAGTGCTATGCATAAGCTTCTACAAGTTCCAGTAATCAGAGAGGACAAATCGGAGAAAGGAAAGAAAGAGGATGCAATTACCACTGACAGTCTTGTCGAGATGTTGAGAGAATTGATAAGGACCATTTGGCGATTCATTCGAGCTGATAAAAATGCCAACATAACCACCGCTTGTCGAAAAAGATCACTTGATGGAGATTGCTTGGATCCAAAGCTTTTAGTGGAAGTCCAAGCAGATCTTCGGAAGAAAGAGAGGAAGCTTAAAGAGCTATTGAGGAGTGAGAACCGGATCATAAAGAGATTCCAAAAGCATGAAGAAGAAGGAGAAGAAGAGGAAGACAGCGGAGATCATGGTCACCATAACTTCTTTTCTCAAGTGGACATCAGATTAGTTTCAAGGGTTTTGAACATGTCAACTATAACACAAGAGCAACTAGTGTGGTGTCAAAGTAAGTTGAGCGAGATACACTTTGTGAAGCGCAAGATTCATGTAGATCCCTCCTTCTTGTTGTTTCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

666

Amino Acids

76.95

Weight (kDa)

4.52

Isoelectric Point (pI)

53.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 429 - 665 6e-80 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 909
AasI GACNNNNNNGTC 1 cut(s) 1571
Acc16I TGCGCA 1 cut(s) 1148
AciI CCGC 3 cut(s) 257, 1647, 1821
AclWI GGATC 8 cut(s) 555, 568, 1202, 1215, 1679, 1692, 1775, 1967
AcsI RAATTY 4 cut(s) 82, 161, 199, 212
AcuI CTGAAG 1 cut(s) 25
AdeI CACNNNGTG 1 cut(s) 1951
AfaI GTAC 6 cut(s) 105, 221, 444, 466, 631, 844
AfiI CCNNNNNNNGG 3 cut(s) 229, 1275, 1300
AflIII ACRYGT 1 cut(s) 1887
AhlI ACTAGT 3 cut(s) 302, 653, 1912
AjuI GAANNNNNNNTTGG 2 cut(s) 1701, 1733
Alw21I GWGCWC 3 cut(s) 403, 987, 1413
Alw26I GTCTC 2 cut(s) 609, 1044
AlwI GGATC 8 cut(s) 555, 568, 1202, 1215, 1679, 1692, 1775, 1967
AoxI GGCC 2 cut(s) 288, 1329
ApoI RAATTY 4 cut(s) 82, 161, 199, 212
Asp700I GAANNNNTTC 1 cut(s) 1440
AspLEI GCGC 2 cut(s) 1149, 1959
AspS9I GGNCC 3 cut(s) 289, 870, 1599
AsuHPI GGTGA 4 cut(s) 188, 281, 647, 1826
AvaII GGWCC 2 cut(s) 870, 1599
BamHI GGATCC 3 cut(s) 560, 1207, 1684
BanII GRGCYC 1 cut(s) 987
BauI CACGAG 1 cut(s) 773
BbsI GAAGAC 2 cut(s) 765, 1821
Bbv12I GWGCWC 3 cut(s) 403, 987, 1413
BccI CCATC 3 cut(s) 479, 776, 1664
BcgI CGANNNNNNTGC 2 cut(s) 34, 68
BclI TGATCA 2 cut(s) 469, 853
BcoDI GTCTC 2 cut(s) 609, 1044
BcuI ACTAGT 3 cut(s) 302, 653, 1912
BfaI CTAG 7 cut(s) 96, 303, 654, 731, 830, 896, 1913
BfmI CTRYAG 2 cut(s) 366, 1196
BglII AGATCT 2 cut(s) 186, 1714
BmcAI AGTACT 1 cut(s) 631
Bme18I GGWCC 2 cut(s) 870, 1599
BmgT120I GGNCC 3 cut(s) 289, 870, 1599
BmiI GGNNCC 5 cut(s) 562, 871, 916, 1209, 1686
BmrI ACTGGG 1 cut(s) 237
BmsI GCATC 1 cut(s) 1540
BmuI ACTGGG 1 cut(s) 237
BpiI GAAGAC 2 cut(s) 765, 1821
BpmI CTGGAG 1 cut(s) 1294
Bpu10I CCTNAGC 1 cut(s) 402
BpuEI CTTGAG 4 cut(s) 709, 971, 1052, 1836
BsaJI CCNNGG 2 cut(s) 918, 1332
BsaWI WCCGGW 3 cut(s) 86, 106, 1764
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 1275, 1300
Bse1I ACTGG 8 cut(s) 232, 252, 637, 651, 970, 1012, 1277, 1508
BseDI CCNNGG 2 cut(s) 918, 1332
BseGI GGATG 5 cut(s) 265, 907, 1057, 1438, 1555
BseLI CCNNNNNNNGG 3 cut(s) 229, 1275, 1300
BseMII CTCAG 5 cut(s) 23, 114, 204, 359, 416
BseNI ACTGG 8 cut(s) 232, 252, 637, 651, 970, 1012, 1277, 1508
BseRI GAGGAG 2 cut(s) 518, 1768
BshFI GGCC 2 cut(s) 290, 1331
BsiHKAI GWGCWC 3 cut(s) 403, 987, 1413
BsiSI CCGG 4 cut(s) 87, 107, 1247, 1765
BslI CCNNNNNNNGG 3 cut(s) 229, 1275, 1300
BsmAI GTCTC 2 cut(s) 609, 1044
BsmI GAATGC 1 cut(s) 1149
BsnI GGCC 2 cut(s) 290, 1331
Bsp1286I GDGCHC 3 cut(s) 403, 987, 1413
BspACI CCGC 3 cut(s) 257, 1647, 1821
BspANI GGCC 2 cut(s) 290, 1331
BspCNI CTCAG 5 cut(s) 22, 115, 203, 358, 415
BspHI TCATGA 2 cut(s) 451, 658
BspLI GGNNCC 5 cut(s) 562, 871, 916, 1209, 1686
BspMAI CTGCAG 1 cut(s) 1200
BspPI GGATC 8 cut(s) 555, 568, 1202, 1215, 1679, 1692, 1775, 1967
BsrI ACTGG 8 cut(s) 232, 252, 637, 651, 970, 1012, 1277, 1508
BssECI CCNNGG 2 cut(s) 918, 1332
BssSI CACGAG 1 cut(s) 773
BssT1I CCWWGG 1 cut(s) 1332
Bst2BI CACGAG 1 cut(s) 773
Bst4CI ACNGT 3 cut(s) 103, 359, 1568
Bst6I CTCTTC 7 cut(s) 56, 57, 495, 609, 675, 828, 1803
BstDEI CTNAG 7 cut(s) 9, 123, 190, 345, 402, 1203, 1214
BstEII GGTNACC 1 cut(s) 1832
BstF5I GGATG 5 cut(s) 265, 907, 1057, 1438, 1555
BstHHI GCGC 2 cut(s) 1149, 1959
BstMAI GTCTC 2 cut(s) 609, 1044
BstMWI GCNNNNNNNGC 2 cut(s) 287, 1744
BstNSI RCATGY 1 cut(s) 1891
BstPI GGTNACC 1 cut(s) 1832
BstSFI CTRYAG 2 cut(s) 366, 1196
BstV2I GAAGAC 2 cut(s) 765, 1821
BstX2I RGATCY 6 cut(s) 186, 560, 1207, 1684, 1714, 1972
BstYI RGATCY 6 cut(s) 186, 560, 1207, 1684, 1714, 1972
BsuRI GGCC 2 cut(s) 290, 1331
BtsCI GGATG 5 cut(s) 265, 907, 1057, 1438, 1555
BtsIMutI CAGTG 4 cut(s) 99, 245, 528, 1560
CciI TCATGA 2 cut(s) 451, 658
CfoI GCGC 2 cut(s) 1149, 1959
Cfr13I GGNCC 3 cut(s) 289, 870, 1599
Csp6I GTAC 6 cut(s) 104, 220, 443, 465, 630, 843
CspCI CAANNNNNGTGG 2 cut(s) 1898, 1933
CviAII CATG 8 cut(s) 452, 659, 1299, 1790, 1829, 1888, 1967, 1995
CviQI GTAC 6 cut(s) 104, 220, 443, 465, 630, 843
DdeI CTNAG 7 cut(s) 9, 123, 190, 345, 402, 1203, 1214
DraI TTTAAA 1 cut(s) 211
DraIII CACNNNGTG 1 cut(s) 1951
DrdI GACNNNNNNGTC 1 cut(s) 1571
DseDI GACNNNNNNGTC 1 cut(s) 1571
Eam1104I CTCTTC 7 cut(s) 56, 57, 495, 609, 675, 828, 1803
EarI CTCTTC 7 cut(s) 56, 57, 495, 609, 675, 828, 1803
Ecl136II GAGCTC 1 cut(s) 985
Eco130I CCWWGG 1 cut(s) 1332
Eco147I AGGCCT 1 cut(s) 1331
Eco24I GRGCYC 1 cut(s) 987
Eco47I GGWCC 2 cut(s) 870, 1599
Eco53kI GAGCTC 1 cut(s) 985
Eco57I CTGAAG 1 cut(s) 25
Eco91I GGTNACC 1 cut(s) 1832
EcoICRI GAGCTC 1 cut(s) 985
EcoO65I GGTNACC 1 cut(s) 1832
EcoRI GAATTC 2 cut(s) 82, 161
EcoT14I CCWWGG 1 cut(s) 1332
EcoT22I ATGCAT 1 cut(s) 1491
EcoT38I GRGCYC 1 cut(s) 987
ErhI CCWWGG 1 cut(s) 1332
FaeI CATG 8 cut(s) 455, 662, 1302, 1793, 1832, 1891, 1970, 1998
FalI AAGNNNNNCTT 2 cut(s) 1701, 1733
FatI CATG 8 cut(s) 451, 658, 1298, 1789, 1828, 1887, 1966, 1994
FbaI TGATCA 2 cut(s) 469, 853
FokI GGATG 5 cut(s) 272, 914, 1064, 1445, 1562
FriOI GRGCYC 1 cut(s) 987
FspBI CTAG 7 cut(s) 96, 303, 654, 731, 830, 896, 1913
FspI TGCGCA 1 cut(s) 1148
GlaI GCGC 2 cut(s) 1148, 1958
GsuI CTGGAG 1 cut(s) 1294
HaeIII GGCC 2 cut(s) 290, 1331
HapII CCGG 4 cut(s) 87, 107, 1247, 1765
HhaI GCGC 2 cut(s) 1149, 1959
Hin1II CATG 8 cut(s) 455, 662, 1302, 1793, 1832, 1891, 1970, 1998
Hin6I GCGC 2 cut(s) 1147, 1957
HinP1I GCGC 2 cut(s) 1147, 1957
HincII GTYRAC 1 cut(s) 1893
HindII GTYRAC 1 cut(s) 1893
HindIII AAGCTT 3 cut(s) 1493, 1691, 1736
HpaII CCGG 4 cut(s) 87, 107, 1247, 1765
HphI GGTGA 4 cut(s) 188, 281, 647, 1826
Hpy166II GTNNAC 3 cut(s) 843, 1858, 1893
Hpy188III TCNNGA 8 cut(s) 452, 572, 659, 731, 988, 1043, 1069, 1576
Hpy8I GTNNAC 3 cut(s) 843, 1858, 1893
Hpy99I CGWCG 2 cut(s) 59, 554
HpyAV CCTTC 6 cut(s) 383, 551, 1319, 1321, 1793, 1990
HpyCH4III ACNGT 3 cut(s) 103, 359, 1568
HpyCH4IV ACGT 1 cut(s) 939
HpyCH4V TGCA 9 cut(s) 491, 772, 1184, 1198, 1385, 1396, 1417, 1489, 1553
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 1744
HpyF3I CTNAG 7 cut(s) 9, 123, 190, 345, 402, 1203, 1214
HpySE526I ACGT 1 cut(s) 939
Hsp92II CATG 8 cut(s) 455, 662, 1302, 1793, 1832, 1891, 1970, 1998
HspAI GCGC 2 cut(s) 1147, 1957
Ksp22I TGATCA 2 cut(s) 469, 853
LmnI GCTCC 3 cut(s) 982, 1336, 1416
LweI GCATC 1 cut(s) 1540
MaeI CTAG 7 cut(s) 96, 303, 654, 731, 830, 896, 1913
MaeII ACGT 1 cut(s) 939
MaeIII GTNAC 3 cut(s) 137, 1130, 1832
MfeI CAATTG 1 cut(s) 428
MflI RGATCY 6 cut(s) 186, 560, 1207, 1684, 1714, 1972
MhlI GDGCHC 3 cut(s) 403, 987, 1413
MlyI GAGTC 4 cut(s) 14, 577, 1046, 1132
MmeI TCCRAC 3 cut(s) 42, 1134, 1293
Mph1103I ATGCAT 1 cut(s) 1491
MroXI GAANNNNTTC 1 cut(s) 1440
MseI TTAA 6 cut(s) 210, 239, 588, 1470, 1740, 1999
MslI CAYNNNNRTG 1 cut(s) 663
MspA1I CMGCKG 1 cut(s) 1821
MspI CCGG 4 cut(s) 87, 107, 1247, 1765
MunI CAATTG 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 1149
MwoI GCNNNNNNNGC 2 cut(s) 287, 1744
NlaIII CATG 8 cut(s) 455, 662, 1302, 1793, 1832, 1891, 1970, 1998
NlaIV GGNNCC 5 cut(s) 562, 871, 916, 1209, 1686
NmuCI GTSAC 1 cut(s) 1832
NsbI TGCGCA 1 cut(s) 1148
NsiI ATGCAT 1 cut(s) 1491
NspI RCATGY 1 cut(s) 1891
PagI TCATGA 2 cut(s) 451, 658
PceI AGGCCT 1 cut(s) 1331
PciI ACATGT 1 cut(s) 1887
PctI GAATGC 1 cut(s) 1149
PdmI GAANNNNTTC 1 cut(s) 1440
PleI GAGTC 4 cut(s) 14, 576, 1045, 1132
PpsI GAGTC 4 cut(s) 14, 576, 1045, 1132
PscI ACATGT 1 cut(s) 1887
PsiI TTATAA 1 cut(s) 909
Psp124BI GAGCTC 1 cut(s) 987
PspEI GGTNACC 1 cut(s) 1832
PspN4I GGNNCC 5 cut(s) 562, 871, 916, 1209, 1686
PspPI GGNCC 3 cut(s) 289, 870, 1599
PstI CTGCAG 1 cut(s) 1200
PsuI RGATCY 6 cut(s) 186, 560, 1207, 1684, 1714, 1972
RsaI GTAC 6 cut(s) 105, 221, 444, 466, 631, 844
RsaNI GTAC 6 cut(s) 104, 220, 443, 465, 630, 843
RseI CAYNNNNRTG 1 cut(s) 663
SacI GAGCTC 1 cut(s) 987
SaqAI TTAA 6 cut(s) 210, 239, 588, 1470, 1740, 1999
Sau96I GGNCC 3 cut(s) 289, 870, 1599
ScaI AGTACT 1 cut(s) 631
SchI GAGTC 4 cut(s) 14, 577, 1046, 1132
SduI GDGCHC 3 cut(s) 403, 987, 1413
SfaNI GCATC 1 cut(s) 1540
SfcI CTRYAG 2 cut(s) 366, 1196
SinI GGWCC 2 cut(s) 870, 1599
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 4 cut(s) 724, 986, 1067, 1851
SmoI CTYRAG 4 cut(s) 724, 986, 1067, 1851
SpeI ACTAGT 3 cut(s) 302, 653, 1912
SseBI AGGCCT 1 cut(s) 1331
SsiI CCGC 3 cut(s) 257, 1647, 1821
SspI AATATT 1 cut(s) 237
SspMI CTAG 7 cut(s) 96, 303, 654, 731, 830, 896, 1913
SstI GAGCTC 1 cut(s) 987
StuI AGGCCT 1 cut(s) 1331
StyI CCWWGG 1 cut(s) 1332
TaaI ACNGT 3 cut(s) 103, 359, 1568
TaiI ACGT 1 cut(s) 942
TaqI TCGA 9 cut(s) 57, 863, 1141, 1226, 1458, 1477, 1575, 1618, 1654
TatI WGTACW 2 cut(s) 442, 629
Tru1I TTAA 6 cut(s) 210, 239, 588, 1470, 1740, 1999
Tru9I TTAA 6 cut(s) 210, 239, 588, 1470, 1740, 1999
TscAI CASTG 4 cut(s) 106, 252, 528, 1567
TseFI GTSAC 1 cut(s) 1832
Tsp45I GTSAC 1 cut(s) 1832
TspGWI ACGGA 2 cut(s) 13, 1107
TspRI CASTG 4 cut(s) 106, 252, 528, 1567
VpaK11BI GGWCC 2 cut(s) 870, 1599
XapI RAATTY 4 cut(s) 82, 161, 199, 212
XbaI TCTAGA 1 cut(s) 730
XceI RCATGY 1 cut(s) 1891
XmnI GAANNNNTTC 1 cut(s) 1440
XspI CTAG 7 cut(s) 96, 303, 654, 731, 830, 896, 1913
ZrmI AGTACT 1 cut(s) 631
Zsp2I ATGCAT 1 cut(s) 1491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.