Rorug03G0013800

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005953
Physical Location & Seq
Reverse (-)
1088835 .. 1091588
2754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0013800.1

Sequence Viewer

Length: 1359 bp
ATGGCCAGGACAGGTGTCTTGGAAGAGACTGATAAAGAAGCAGCCATGGAGATAAAGAAGCAGTTGGGGAAACTGGTCAAGGAGATTGTTGAAGAAGAAGACTACAGGGTAGAGACTGCTGATGAGGCTATAAGGGCCTTGTCTTCTTTAAAGAACTTGCAGAGGTCTACAAAGTCTCTCTCTTTTAAACTTGGTGTTCTTGATGTTCCTCAAGAGTTTAGGTGTCCTATTTCTGGAGAGCTCATGACTGATCCTGTTGTCTTGGCCACTGGACAGACTTATGATAGGCCATTCATCCAGAGGTTGTTGAATGAAGGCAGCCGGACATGCCCTCAGACCAAGCAAGTCCTTGCTCACACTATCCTGACCCCAAATAACTTGGTCCAAGAACTGATTTCACAATGGTGCAAGGAGCATGGTATTGAGTCTCCGAAACCTGTTCAGGATACTGGTGAGGAAGTTGTCACCAACGCAGACCGAAACTATCTGAATGCACTGCTCAATAAGACTTCATCATCATCCCTGGCTGATCAAAAAGAAGCAGCAAAGGAACTTCGGCTGCTAACAAAGAGGAGTTCATCATTTCGGGCATTGTTTGGCGAGTCTGCTGAGATCATTCCACAACTGCTGAGGCCACTTTCACAAGGCCAGGTTGCCACTCATCCTGACCTTCAAGAAGACTTGATCACGACTCTACTAAACCTTTCGATTCATGACAACAATAAGAGATTAGTTGCTGAAAACCCCTTGGTGATCCCTCTCCTTATTGATTCCTTAATATCTGGAACAATTCAAACAAAAAGCAATGCTGCTGCAACTTTCTTCACACTATCAGCCATCGATTCCAGCAAGATTGTTATCGGAACATCTGGTGCTCTCAAACCTTTGATTGACCTGTTGCATGAGGGGCATCCATTAGCCATGAAGGATGTTGCCACGGCCATATTCCGTCTATGTACAGACCTTGAAAATAGAGGAAGAGCCGTCAATGATGGAGTGGTGTGGGTAATTCTGAATAAGATAATGGATGGTGTATTGGTCGCTGAGTTGCTGGCTATACTCGCAATAATTTCAACCAATCAGAAGGCTATTCAGGAAATGTGTGAGCTTGGCGCTGTGTCTTGCTTGATTAGGATCATTAGGGAGAGTGATTGTGAGCGTGACAAGGAGAACTGCATCGTAATCCTTTACAATATCTGCTTTAGTGGTCGCTCCAAATTGAAGGAGATTAAGGACGAGGAAATGGCAAATGGTACATTTTCTAGACTTGCACAGAGTGGAACTTCAAGAGCTAAGAGGAAAGCCATTGGAATTCTTGAGAGGTTGAATAAAACTGGTTCAGTAACACACTCTGCTTGA

Protein Analysis

452

Amino Acids

49.76

Weight (kDa)

6.08

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 69 - 139 3.3e-20 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 167
AclWI GGATC 3 cut(s) 245, 748, 1142
AcoI YGGCCR 3 cut(s) 3, 264, 939
AcsI RAATTY 1 cut(s) 1311
AdeI CACNNNGTG 1 cut(s) 1277
AfaI GTAC 2 cut(s) 958, 1255
AfiI CCNNNNNNNGG 1 cut(s) 233
AgsI TTSAA 9 cut(s) 92, 310, 674, 794, 968, 1074, 1222, 1287, 1327
AjnI CCWGG 3 cut(s) 5, 522, 648
AleI CACNNNNGTG 1 cut(s) 403
AluBI AGCT 3 cut(s) 241, 1108, 1292
AluI AGCT 3 cut(s) 241, 1108, 1292
Alw21I GWGCWC 2 cut(s) 243, 877
Alw26I GTCTC 4 cut(s) 20, 107, 180, 432
AlwI GGATC 3 cut(s) 245, 748, 1142
AoxI GGCC 7 cut(s) 3, 135, 264, 287, 632, 646, 939
ApeKI GCWGC 6 cut(s) 41, 318, 542, 559, 809, 812
ApoI RAATTY 1 cut(s) 1311
AspLEI GCGC 1 cut(s) 1115
AspS9I GGNCC 2 cut(s) 135, 382
AsuHPI GGTGA 3 cut(s) 457, 464, 763
AvaII GGWCC 1 cut(s) 382
BalI TGGCCA 2 cut(s) 5, 266
BanII GRGCYC 1 cut(s) 243
BbsI GAAGAC 3 cut(s) 105, 135, 684
Bbv12I GWGCWC 2 cut(s) 243, 877
BbvCI CCTCAGC 1 cut(s) 629
BbvI GCAGC 6 cut(s) 53, 330, 546, 554, 796, 799
BccI CCATC 3 cut(s) 845, 986, 1022
BceAI ACGGC 2 cut(s) 954, 968
BciT130I CCWGG 3 cut(s) 7, 524, 650
BciVI GTATCC 1 cut(s) 439
BclI TGATCA 2 cut(s) 529, 684
BcoDI GTCTC 4 cut(s) 20, 107, 180, 432
BfaI CTAG 1 cut(s) 1263
BfmI CTRYAG 1 cut(s) 103
BfoI RGCGCY 1 cut(s) 1116
BfuI GTATCC 1 cut(s) 439
BisI GCNGC 6 cut(s) 42, 319, 543, 560, 810, 813
BlsI GCNGC 6 cut(s) 43, 320, 544, 561, 811, 814
Bme1390I CCNGG 3 cut(s) 7, 524, 650
Bme18I GGWCC 1 cut(s) 382
BmgT120I GGNCC 2 cut(s) 135, 382
BmrFI CCNGG 3 cut(s) 7, 524, 650
BmsI GCATC 2 cut(s) 919, 1185
BoxI GACNNNNGTC 1 cut(s) 14
BpiI GAAGAC 3 cut(s) 105, 135, 684
BpmI CTGGAG 1 cut(s) 255
Bpu10I CCTNAGC 1 cut(s) 629
BpuEI CTTGAG 2 cut(s) 195, 1337
Bsa29I ATCGAT 1 cut(s) 840
BsaBI GATNNNNATC 2 cut(s) 857, 1133
BsaJI CCNNGG 4 cut(s) 45, 522, 747, 936
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse1I ACTGG 4 cut(s) 78, 274, 454, 1339
Bse3DI GCAATG 1 cut(s) 811
Bse8I GATNNNNATC 2 cut(s) 857, 1133
BseBI CCWGG 3 cut(s) 7, 524, 650
BseCI ATCGAT 1 cut(s) 840
BseDI CCNNGG 4 cut(s) 45, 522, 747, 936
BseGI GGATG 6 cut(s) 294, 518, 661, 910, 934, 1033
BseJI GATNNNNATC 2 cut(s) 857, 1133
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMI GCAATG 1 cut(s) 811
BseMII CTCAG 4 cut(s) 347, 600, 620, 1035
BseNI ACTGG 4 cut(s) 78, 274, 454, 1339
BseRI GAGGAG 1 cut(s) 586
BseXI GCAGC 6 cut(s) 53, 330, 546, 554, 796, 799
BshFI GGCC 7 cut(s) 5, 137, 266, 289, 634, 648, 941
BshVI ATCGAT 1 cut(s) 840
BsiHKAI GWGCWC 2 cut(s) 243, 877
BsiSI CCGG 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 4 cut(s) 20, 107, 180, 432
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 7 cut(s) 5, 137, 266, 289, 634, 648, 941
Bsp1286I GDGCHC 2 cut(s) 243, 877
Bsp1407I TGTACA 1 cut(s) 956
Bsp143I GATC 6 cut(s) 250, 529, 612, 684, 753, 1134
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 7 cut(s) 5, 137, 266, 289, 634, 648, 941
BspCNI CTCAG 4 cut(s) 346, 601, 621, 1036
BspDI ATCGAT 1 cut(s) 840
BspHI TCATGA 2 cut(s) 243, 712
BspPI GGATC 3 cut(s) 245, 748, 1142
BspQI GCTCTTC 1 cut(s) 973
BsrDI GCAATG 1 cut(s) 811
BsrGI TGTACA 1 cut(s) 956
BsrI ACTGG 4 cut(s) 78, 274, 454, 1339
BssECI CCNNGG 4 cut(s) 45, 522, 747, 936
BssMI GATC 6 cut(s) 250, 529, 612, 684, 753, 1134
BssT1I CCWWGG 2 cut(s) 45, 747
Bst2UI CCWGG 3 cut(s) 7, 524, 650
Bst6I CTCTTC 2 cut(s) 18, 973
BstAUI TGTACA 1 cut(s) 956
BstC8I GCNNGC 1 cut(s) 1053
BstDEI CTNAG 5 cut(s) 333, 609, 629, 1044, 1293
BstDSI CCRYGG 2 cut(s) 45, 936
BstF5I GGATG 6 cut(s) 294, 518, 661, 910, 934, 1033
BstH2I RGCGCY 1 cut(s) 1116
BstHHI GCGC 1 cut(s) 1115
BstKTI GATC 6 cut(s) 253, 532, 615, 687, 756, 1137
BstMAI GTCTC 4 cut(s) 20, 107, 180, 432
BstMBI GATC 6 cut(s) 250, 529, 612, 684, 753, 1134
BstMWI GCNNNNNNNGC 5 cut(s) 125, 134, 327, 907, 1061
BstNI CCWGG 3 cut(s) 7, 524, 650
BstNSI RCATGY 1 cut(s) 330
BstPAI GACNNNNGTC 1 cut(s) 14
BstSCI CCNGG 3 cut(s) 5, 522, 648
BstSFI CTRYAG 1 cut(s) 103
BstV1I GCAGC 6 cut(s) 53, 330, 546, 554, 796, 799
BstV2I GAAGAC 3 cut(s) 105, 135, 684
Bsu15I ATCGAT 1 cut(s) 840
BsuI GTATCC 1 cut(s) 439
BsuRI GGCC 7 cut(s) 5, 137, 266, 289, 634, 648, 941
BsuTUI ATCGAT 1 cut(s) 840
BtgI CCRYGG 2 cut(s) 45, 936
BtsCI GGATG 6 cut(s) 294, 518, 661, 910, 934, 1033
BtsI GCAGTG 1 cut(s) 494
BtsIMutI CAGTG 2 cut(s) 267, 494
Cac8I GCNNGC 1 cut(s) 1053
CciI TCATGA 2 cut(s) 243, 712
CfoI GCGC 1 cut(s) 1115
Cfr13I GGNCC 2 cut(s) 135, 382
ClaI ATCGAT 1 cut(s) 840
Csp6I GTAC 2 cut(s) 957, 1254
CviAII CATG 7 cut(s) 46, 244, 327, 416, 713, 902, 922
CviQI GTAC 2 cut(s) 957, 1254
DdeI CTNAG 5 cut(s) 333, 609, 629, 1044, 1293
DpnI GATC 6 cut(s) 252, 531, 614, 686, 755, 1136
DpnII GATC 6 cut(s) 250, 529, 612, 684, 753, 1134
DraI TTTAAA 2 cut(s) 150, 187
DraIII CACNNNGTG 1 cut(s) 1277
EaeI YGGCCR 3 cut(s) 3, 264, 939
Eam1104I CTCTTC 2 cut(s) 18, 973
EarI CTCTTC 2 cut(s) 18, 973
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 45, 747
Eco24I GRGCYC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 241
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 135
EcoRI GAATTC 1 cut(s) 1311
EcoRII CCWGG 3 cut(s) 5, 522, 648
EcoT14I CCWWGG 2 cut(s) 45, 747
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 2 cut(s) 45, 747
FaeI CATG 7 cut(s) 49, 247, 330, 419, 716, 905, 925
FatI CATG 7 cut(s) 45, 243, 326, 415, 712, 901, 921
FbaI TGATCA 2 cut(s) 529, 684
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 6 cut(s) 42, 319, 543, 560, 810, 813
FokI GGATG 6 cut(s) 281, 505, 648, 897, 941, 1040
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 6 cut(s) 42, 319, 543, 560, 810, 813
FspBI CTAG 1 cut(s) 1263
GlaI GCGC 1 cut(s) 1114
GluI GCNGC 6 cut(s) 42, 319, 543, 560, 810, 813
GsuI CTGGAG 1 cut(s) 255
HaeII RGCGCY 1 cut(s) 1116
HaeIII GGCC 7 cut(s) 5, 137, 266, 289, 634, 648, 941
HapII CCGG 1 cut(s) 322
HhaI GCGC 1 cut(s) 1115
Hin1II CATG 7 cut(s) 49, 247, 330, 419, 716, 905, 925
Hin6I GCGC 1 cut(s) 1113
HinP1I GCGC 1 cut(s) 1113
HinfI GANTC 6 cut(s) 425, 602, 691, 709, 770, 842
HpaII CCGG 1 cut(s) 322
HphI GGTGA 3 cut(s) 457, 464, 763
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 6 cut(s) 336, 432, 489, 863, 1014, 1083
Hpy8I GTNNAC 1 cut(s) 168
HpyAV CCTTC 5 cut(s) 308, 680, 919, 1078, 1216
HpyCH4V TGCA 7 cut(s) 160, 408, 494, 815, 901, 1176, 1271
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 134, 327, 907, 1061
HpyF3I CTNAG 5 cut(s) 333, 609, 629, 1044, 1293
Hsp92II CATG 7 cut(s) 49, 247, 330, 419, 716, 905, 925
HspAI GCGC 1 cut(s) 1113
Ksp22I TGATCA 2 cut(s) 529, 684
Kzo9I GATC 6 cut(s) 250, 529, 612, 684, 753, 1134
LguI GCTCTTC 1 cut(s) 973
LmnI GCTCC 2 cut(s) 412, 1217
Lsp1109I GCAGC 6 cut(s) 53, 330, 546, 554, 796, 799
LweI GCATC 2 cut(s) 919, 1185
MaeI CTAG 1 cut(s) 1263
MaeIII GTNAC 3 cut(s) 463, 1160, 1342
MalI GATC 6 cut(s) 252, 531, 614, 686, 755, 1136
MboI GATC 6 cut(s) 250, 529, 612, 684, 753, 1134
MboII GAAGA 8 cut(s) 35, 104, 107, 110, 135, 689, 814, 990
MhlI GDGCHC 2 cut(s) 243, 877
MlsI TGGCCA 2 cut(s) 5, 266
MluCI AATT 5 cut(s) 789, 1008, 1068, 1217, 1311
MluNI TGGCCA 2 cut(s) 5, 266
MlyI GAGTC 3 cut(s) 434, 611, 685
Mox20I TGGCCA 2 cut(s) 5, 266
MscI TGGCCA 2 cut(s) 5, 266
MseI TTAA 4 cut(s) 149, 186, 776, 1230
MslI CAYNNNNRTG 1 cut(s) 403
Msp20I TGGCCA 2 cut(s) 5, 266
MspI CCGG 1 cut(s) 322
MspR9I CCNGG 3 cut(s) 7, 524, 650
Mva1269I GAATGC 1 cut(s) 496
MvaI CCWGG 3 cut(s) 7, 524, 650
MwoI GCNNNNNNNGC 5 cut(s) 125, 134, 327, 907, 1061
NcoI CCATGG 1 cut(s) 45
NdeII GATC 6 cut(s) 250, 529, 612, 684, 753, 1134
NlaIII CATG 7 cut(s) 49, 247, 330, 419, 716, 905, 925
NmuCI GTSAC 2 cut(s) 463, 1160
NspI RCATGY 1 cut(s) 330
OliI CACNNNNGTG 1 cut(s) 403
PagI TCATGA 2 cut(s) 243, 712
PciSI GCTCTTC 1 cut(s) 973
PctI GAATGC 1 cut(s) 496
PfeI GAWTC 3 cut(s) 709, 770, 842
PkrI GCNGC 6 cut(s) 43, 320, 544, 561, 811, 814
PleI GAGTC 3 cut(s) 433, 610, 685
PpsI GAGTC 3 cut(s) 433, 610, 685
PshAI GACNNNNGTC 1 cut(s) 14
Psp124BI GAGCTC 1 cut(s) 243
Psp6I CCWGG 3 cut(s) 5, 522, 648
PspGI CCWGG 3 cut(s) 5, 522, 648
PspPI GGNCC 2 cut(s) 135, 382
RsaI GTAC 2 cut(s) 958, 1255
RsaNI GTAC 2 cut(s) 957, 1254
RseI CAYNNNNRTG 1 cut(s) 403
SacI GAGCTC 1 cut(s) 243
SapI GCTCTTC 1 cut(s) 973
SaqAI TTAA 4 cut(s) 149, 186, 776, 1230
SatI GCNGC 6 cut(s) 42, 319, 543, 560, 810, 813
Sau3AI GATC 6 cut(s) 250, 529, 612, 684, 753, 1134
Sau96I GGNCC 2 cut(s) 135, 382
SchI GAGTC 3 cut(s) 434, 611, 685
ScrFI CCNGG 3 cut(s) 7, 524, 650
SduI GDGCHC 2 cut(s) 243, 877
SfaNI GCATC 2 cut(s) 919, 1185
SfcI CTRYAG 1 cut(s) 103
SinI GGWCC 1 cut(s) 382
SmiMI CAYNNNNRTG 1 cut(s) 403
SmlI CTYRAG 2 cut(s) 210, 1316
SmoI CTYRAG 2 cut(s) 210, 1316
Sse9I AATT 5 cut(s) 789, 1008, 1068, 1217, 1311
SspMI CTAG 1 cut(s) 1263
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 3 cut(s) 5, 522, 648
StyI CCWWGG 2 cut(s) 45, 747
TaqI TCGA 2 cut(s) 707, 840
TaqII GACCGA 1 cut(s) 492
TasI AATT 5 cut(s) 789, 1008, 1068, 1217, 1311
TatI WGTACW 1 cut(s) 956
TfiI GAWTC 3 cut(s) 709, 770, 842
Tru1I TTAA 4 cut(s) 149, 186, 776, 1230
Tru9I TTAA 4 cut(s) 149, 186, 776, 1230
TscAI CASTG 2 cut(s) 274, 501
TseFI GTSAC 2 cut(s) 463, 1160
TseI GCWGC 6 cut(s) 41, 318, 542, 559, 809, 812
Tsp45I GTSAC 2 cut(s) 463, 1160
TspDTI ATGAA 6 cut(s) 283, 327, 501, 567, 701, 938
TspGWI ACGGA 1 cut(s) 938
TspRI CASTG 2 cut(s) 274, 501
VpaK11BI GGWCC 1 cut(s) 382
XapI RAATTY 1 cut(s) 1311
XbaI TCTAGA 1 cut(s) 1262
XceI RCATGY 1 cut(s) 330
XmiI GTMKAC 1 cut(s) 167
XspI CTAG 1 cut(s) 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.