Rroxscaffold_6G00422660

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005953
Physical Location & Seq
Reverse (-)
43467103 .. 43475645
8543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00422660.1

Sequence Viewer

Length: 3294 bp
ATGTCGAATTTGTGCAGGGAATTGGTGTTACTGGTCCTCCAATTCCTTGACGAGGAGAAGTTCAGAGGGGCCATGCATAAGCTTGAGCAGGAATCAGGGTTCTTTTTCAACATGAAGTACTTTGAGGAGAAGATTTGGACTGGAGAATGGGAAGAAGTTGAAAAGTATTTATCAGGATATACGAAATTCAATGACAATCGATTTTCACTGAAAATAATGTTTGAAATATCAAAGCAGAAATATCTGGAAGCCCTCGATCGGAACGAGAAGGTAGAGGCCATTGACATCTTAGTCAATGATCTCCAGAAGTTTGCTCCTTATAATCAAGAACTTTACAAGGAGATCACACAGCTTTTAACCCTCCGAAACTTTCGGGAAAATGCACATCTCACTCAGTACGGCGACACCAGATCAGCTCGTCATAGAATGGTGGTAGAGCTCAAAAAGCTCATAAAAGCAAACCCTCTATTGAAGGATAAGCTTTATTTTCCTTCTGTGAGGTCAGCCAGACTGCGGACTCTGATTAATCAGAGTCTTAACTGGCAGCACCAGCAATGCAAGGACCCGGTTCCGAATCCAGAAATAAAAACTCTGTTCATAGATCATTACTGTCCACCACCTCTAACTGGTCCTCTTGCTCTAGCAAATGTCAATCAGCCATTTCCTGGAATTGAATTTCCTGCTCCTTTTCCCTCAATGGAAGGAGCTATTGTTCCGTATCAGGCCCCTGGAGAAGTTCCAAGCCTAAAGTGGCCAAGAACAGCTCTGGAAACTTCTGGTGGGGTTGATCCTTCTAGTACTGGTTTTACTGCTATTGACCGGATGGCGAAAGAACTGCAACATGTTCGATCGAACTTGGAGGGTACCTTTCAAACTTCTAGACTGCTTCAAACTTCCTGGTTGGTGGAGAACCTACCGAGAAATGTGGCTTATAGCATGCGTCAAGGATCTACAGTCACAAGCATGGATTTTCATCCTTATCACCATACATTGCTCCTAGTTGGTGCTAGCAATGGTGAAATTAGTCTTTGGGAACTTGGAACTCGGCAGAGGGTGGCTTCGCAGCCATTCAGAATATGGAATATGGCAGCACGTTCATCAGAACTTCAGAGCACACTTGCCAATGAAGACACATCTATATCTGTGAGCCGTGTTACATGGAGTCCAGATGGGAACTTTTTTGGAGTTGCATTTACTAAAAATTTGATTCACTTGTATGCTCTTTCTGGAACTAGTGATCTATGCCGGTATTTGGAGGTTGACGCCCATGATGGTGGTGTGAATGACTTGGCTTTTGATTATCGAAACAACGAACTGTGTGTTATCACATGTGGAGATGACAAGCTAATAAAGGTATGGGATATAGCAGGACGAAACTTATATAACTTTGATGGTCATGAAGCACCAGTCTATTCAGTGTGTCCTTATCATAACAAGGACGTTCAGTACATATATTCAACTTCTACTGATGGGAAGATAAAGACCTGGATGTACGACAATGGGGGTCCTAGATCTGGCTGTGATGCTCCTGGCAACTGGTGTACGACATTGCTTTTCACTCCGGATGGTAACCGAATGTTCTCTTGTGGAACAAGTAAAGAGGGAGACACTTTTCTAGTTCAATGGAGCGAAAATGAAGGAATAATTAGAGAAGGAGCAAAAAAGAGAATTTATTCTGGGTTCGGTAAGAAATCTGAAGGGGTTGTGCATTTTGACATAACGAAAAAGCATTTTTTGGCTGTGGGTGAAGATAGCCAGATCAAGTATTGGGATATGGACCATGATGATGTTCTGACTAGCACGGACGCCCAGGGTGAACTGCCGAGTCGTCCTCGCTTGAGATTCAACAAGGAAGGTAGTCTTCTTGCTGTCACAACGGCACATGAGGGATTCATGATACTTGCAAATGGAATCGGACGTCTGAGAGTTCATGGAGTCACTGCAACTTATTGTGACAGGTCGAAAGATCCTACTGAATCCACTGCAAACAAGGCATCTGGCTCATCTTCGGTTGCAAATGTCAGTGAGGCCAATAGGAAATCTGACAGGGGCTCTCCTGCTAGGTCTACAACATCTCAGAATGGAGCTAATGCCAAGAGTAGAAGTTTCAAGAAACGGAAAACTGTGGGGGAGGTAATCAATAAATCCAAACCTTGGCAGTTGACTGACATTCGGGATCCTGTAAGGTGTCGTTTAGGTGTTATGCCTGAAACAGATAGTTCCTGCAAGGTTGTTCGACTCCTATATACAAGTAACGGTACTGCCATCTTAGCACTTGGGTCAAATGGTGTACAGAGACTGTGGAGGTGGCCCCGTACTAGAGGTCGAATTTCAAAGGCCAGCGCCAGTGTTGTTCCGCAGCTGGTGAAAGGGGACAATGGTCCTGTTATGGCAAATGATGTCTCAGGTGTCAACCTTGAAGAGGCGGTTCCCTGCATAGCGCTCTCAAAGAATGACGCGTATGTAATGTCAGCTTATGGTGGAAGGATCAAATTATTTGACATTTATAATTTGGCGGTAATGATAACTTTCGGGGAACTTCCCCCTGCACCAACTGAAATTGCATTCAATCCTCGGGATAATAATATCATGGCCATTGGAATGCAAGATTGCACCATACAGATTTTCTTTTGTAGACTTAATGAGGTAAGAGCAATCTTGAAGTTTCACAAGAAGCGGATAACTGGTTTAGTTTTCTCGTTCTTTCTTGCTATACTCGTGTCAGCTAGTGCCGACGGTCAAATATGTGTGTGGAGCATTGATCAATGGGAGAAGAAACAATCTGTTGCCTTATGTTTGGGTTCTGAAAAGACACCTGAGGGTGCCACTCAATTGCAGTTTCATTGCAACCAAGTCCGCTTACTGGTGTTTCATGAAACACAACTGGCAGTTTATGATGCCTCAAAGATGAAGCTCCTCCGGCAGTGGCTACCACATGGTATGCTTTCTGCTCCCATACGTCATGCAGCATACTCCTTTGACAGCAGACTGGTTTATACCGCCTTCAGTGATGGTAACATTGGAGTATTTGATGCCAATAGCCTAAGACTGAGATGTCGTATTGCTCCATCAGCATACTTACCGGAAGCAGTCTTGAAGAGGACCGATGAATTGTATCCTCTTGTCATTGCAGGAAATCCCCGTGTACAAAACCAGTTTGCTGTTGGGTTGTCTGATGGTACTGTTAAGGTCATTGAGCCATTATTTGGTGACTGGGGGTCCTCCCCACCTCCTGATGATGAAGCTTCATCAAGTGCCATCAACAACCACTCGTCCAGACAAGTACAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

1097

Amino Acids

122.87

Weight (kDa)

8.3

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 6 - 34 1.2e-08 LisH-like dimerisation domain
TPR1-like_2nd PF21889 37 - 161 2.1e-52 TPR1-like, CTLH-containing domain
zf_topless PF21359 164 - 204 1.3e-22 TOPLESS, zinc finger domain
WD40_Prp19 PF24814 317 - 455 8.3e-08 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 317 - 530 3.9e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 357 - 534 3.8e-16 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 358 - 530 1.5e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 379 - 598 2e-19 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 381 - 629 1.1e-16 THOC3 beta-propeller domain
WD40_Prp19 PF24814 422 - 531 3.2e-07 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 436 - 500 2.3e-07 WDHD1 first WD40 domain
WD40_WDHD1_1st PF24817 799 - 1014 3.8e-14 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 804 - 928 2.3e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 807 - 924 2.5e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 808 - 1021 6.1e-09 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 808 - 926 1.7e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 809 - 1018 7.2e-13 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 810 - 1010 5.7e-08 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 813 - 921 6.1e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 829 - 924 8.6e-07 WDR90/POC16, second beta-propeller
WD40_Prp19 PF24814 852 - 1015 6.9e-10 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 321, 2508
AasI GACNNNNNNGTC 2 cut(s) 290, 3054
AatII GACGTC 1 cut(s) 1921
Acc65I GGTACC 1 cut(s) 863
AccB1I GGYRCC 2 cut(s) 863, 2822
AccB7I CCANNNNNTGG 3 cut(s) 665, 2154, 3206
AccI GTMKAC 2 cut(s) 2066, 2635
AccII CGCG 1 cut(s) 2459
AccIII TCCGGA 1 cut(s) 1561
AciI CCGC 7 cut(s) 514, 2357, 2426, 2516, 2677, 2857, 3000
AclWI GGATC 6 cut(s) 782, 955, 1961, 2171, 2184, 2495
AcoI YGGCCR 2 cut(s) 752, 2592
AcsI RAATTY 6 cut(s) 7, 185, 674, 1201, 1668, 2328
AcuI CTGAAG 3 cut(s) 1091, 1716, 2989
AcyI GRCGYC 3 cut(s) 1263, 1806, 1918
AfeI AGCGCT 1 cut(s) 2442
AflIII ACRYGT 3 cut(s) 841, 1328, 2457
AhdI GACNNNNNGTC 1 cut(s) 3217
AhlI ACTAGT 1 cut(s) 1232
AjnI CCWGG 6 cut(s) 664, 727, 896, 1484, 1528, 1809
AloI GAACNNNNNNTCC 4 cut(s) 83, 115, 696, 728
Alw21I GWGCWC 2 cut(s) 441, 1115
Alw26I GTCTC 3 cut(s) 1599, 2290, 2407
AlwI GGATC 6 cut(s) 782, 955, 1961, 2171, 2184, 2495
AlwNI CAGNNNCTG 1 cut(s) 2299
Ama87I CYCGRG 1 cut(s) 2574
Aor13HI TCCGGA 1 cut(s) 1561
Aor51HI AGCGCT 1 cut(s) 2442
AoxI GGCC 8 cut(s) 69, 276, 723, 752, 2028, 2309, 2337, 2592
ApeKI GCWGC 5 cut(s) 544, 1063, 1088, 2359, 2966
ApoI RAATTY 6 cut(s) 7, 185, 674, 1201, 1668, 2328
ArsI GACNNNNNNTTYG 2 cut(s) 1277, 1309
AseI ATTAAT 1 cut(s) 525
Asp700I GAANNNNTTC 1 cut(s) 1672
Asp718I GGTACC 1 cut(s) 863
AspLEI GCGC 2 cut(s) 2345, 2443
AsuC2I CCSGG 1 cut(s) 566
AsuHPI GGTGA 6 cut(s) 974, 1028, 1757, 1826, 2377, 3221
AsuNHI GCTAGC 1 cut(s) 1007
AvaI CYCGRG 1 cut(s) 2574
AvaII GGWCC 8 cut(s) 34, 562, 629, 1505, 1777, 2381, 3102, 3219
AxyI CCTNAGG 1 cut(s) 2817
BalI TGGCCA 2 cut(s) 754, 2594
BamHI GGATCC 1 cut(s) 2176
BanI GGYRCC 2 cut(s) 863, 2822
BanII GRGCYC 2 cut(s) 441, 2054
BauI CACGAG 1 cut(s) 2717
BbsI GAAGAC 2 cut(s) 1134, 1853
Bbv12I GWGCWC 2 cut(s) 441, 1115
BbvI GCAGC 5 cut(s) 556, 1075, 1100, 2371, 2978
BceAI ACGGC 3 cut(s) 415, 1134, 1893
BcgI CGANNNNNNTGC 4 cut(s) 817, 827, 851, 861
BciT130I CCWGG 6 cut(s) 666, 729, 898, 1486, 1530, 1811
BciVI GTATCC 1 cut(s) 3126
BclI TGATCA 1 cut(s) 2761
BcnI CCSGG 1 cut(s) 566
BcoDI GTCTC 3 cut(s) 1599, 2290, 2407
BcuI ACTAGT 1 cut(s) 1232
BfmI CTRYAG 1 cut(s) 951
BfoI RGCGCY 2 cut(s) 2346, 2444
BfuI GTATCC 1 cut(s) 3126
BglII AGATCT 1 cut(s) 1511
BisI GCNGC 5 cut(s) 545, 1064, 1089, 2360, 2967
BlsI GCNGC 5 cut(s) 546, 1065, 1090, 2361, 2968
BmcAI AGTACT 2 cut(s) 119, 799
Bme1390I CCNGG 7 cut(s) 566, 666, 729, 898, 1486, 1530, 1811
Bme18I GGWCC 8 cut(s) 34, 562, 629, 1505, 1777, 2381, 3102, 3219
BmeRI GACNNNNNGTC 1 cut(s) 3217
BmeT110I CYCGRG 1 cut(s) 2574
BmrFI CCNGG 7 cut(s) 566, 666, 729, 898, 1486, 1530, 1811
BmrI ACTGGG 1 cut(s) 3223
BmsI GCATC 4 cut(s) 1513, 2003, 2887, 3022
BmtI GCTAGC 1 cut(s) 1011
BmuI ACTGGG 1 cut(s) 3223
BoxI GACNNNNGTC 1 cut(s) 2379
BpiI GAAGAC 2 cut(s) 1134, 1853
BplI GAGNNNNNCTC 2 cut(s) 1816, 1848
BpmI CTGGAG 3 cut(s) 162, 287, 750
BpuEI CTTGAG 2 cut(s) 104, 1858
BpuMI CCSGG 1 cut(s) 566
Bsa29I ATCGAT 1 cut(s) 199
BsaBI GATNNNNATC 1 cut(s) 972
BsaHI GRCGYC 3 cut(s) 1263, 1806, 1918
BsaJI CCNNGG 5 cut(s) 727, 1809, 1810, 2153, 2573
BsaWI WCCGGW 3 cut(s) 819, 1561, 3082
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 696, 726
Bse118I RCCGGY 1 cut(s) 1245
Bse21I CCTNAGG 1 cut(s) 2817
Bse3DI GCAATG 6 cut(s) 560, 989, 1018, 1547, 2842, 3126
Bse8I GATNNNNATC 1 cut(s) 972
BseAI TCCGGA 1 cut(s) 1561
BseBI CCWGG 6 cut(s) 666, 729, 898, 1486, 1530, 1811
BseCI ATCGAT 1 cut(s) 199
BseDI CCNNGG 5 cut(s) 727, 1809, 1810, 2153, 2573
BseGI GGATG 4 cut(s) 828, 973, 1494, 1570
BseJI GATNNNNATC 1 cut(s) 972
BseMI GCAATG 6 cut(s) 560, 989, 1018, 1547, 2842, 3126
BseMII CTCAG 6 cut(s) 407, 1913, 2090, 2418, 2808, 3041
BseRI GAGGAG 3 cut(s) 68, 140, 2906
BseXI GCAGC 5 cut(s) 556, 1075, 1100, 2371, 2978
BsgI GTGCAG 2 cut(s) 34, 2532
Bsh1236I CGCG 1 cut(s) 2459
Bsh1285I CGRYCG 2 cut(s) 259, 851
BshFI GGCC 8 cut(s) 71, 278, 725, 754, 2030, 2311, 2339, 2594
BshNI GGYRCC 2 cut(s) 863, 2822
BshVI ATCGAT 1 cut(s) 199
BsiEI CGRYCG 2 cut(s) 259, 851
BsiHKAI GWGCWC 2 cut(s) 441, 1115
BsiHKCI CYCGRG 1 cut(s) 2574
BsiSI CCGG 6 cut(s) 566, 820, 1246, 1562, 2920, 3083
BslFI GGGAC 1 cut(s) 2387
BsmAI GTCTC 3 cut(s) 1599, 2290, 2407
BsmFI GGGAC 1 cut(s) 2387
BsmI GAATGC 2 cut(s) 2564, 2607
BsnI GGCC 8 cut(s) 71, 278, 725, 754, 2030, 2311, 2339, 2594
BsoBI CYCGRG 1 cut(s) 2574
Bsp1286I GDGCHC 3 cut(s) 441, 1115, 2054
Bsp13I TCCGGA 1 cut(s) 1561
Bsp1407I TGTACA 2 cut(s) 2290, 3145
BspACI CCGC 7 cut(s) 514, 2357, 2426, 2516, 2677, 2857, 3000
BspANI GGCC 8 cut(s) 71, 278, 725, 754, 2030, 2311, 2339, 2594
BspCNI CTCAG 6 cut(s) 406, 1914, 2089, 2417, 2809, 3042
BspDI ATCGAT 1 cut(s) 199
BspEI TCCGGA 1 cut(s) 1561
BspFNI CGCG 1 cut(s) 2459
BspHI TCATGA 3 cut(s) 1396, 1893, 2872
BspOI GCTAGC 1 cut(s) 1011
BspPI GGATC 6 cut(s) 782, 955, 1961, 2171, 2184, 2495
BspT107I GGYRCC 2 cut(s) 863, 2822
BsrDI GCAATG 6 cut(s) 560, 989, 1018, 1547, 2842, 3126
BsrFI RCCGGY 1 cut(s) 1245
BsrGI TGTACA 2 cut(s) 2290, 3145
BssAI RCCGGY 1 cut(s) 1245
BssECI CCNNGG 5 cut(s) 727, 1809, 1810, 2153, 2573
BssNI GRCGYC 3 cut(s) 1263, 1806, 1918
BssSI CACGAG 1 cut(s) 2717
BssT1I CCWWGG 1 cut(s) 2153
Bst2BI CACGAG 1 cut(s) 2717
Bst2UI CCWGG 6 cut(s) 666, 729, 898, 1486, 1530, 1811
Bst4CI ACNGT 8 cut(s) 611, 955, 1317, 2125, 2258, 2301, 2738, 3184
Bst6I CTCTTC 2 cut(s) 2415, 3092
BstACI GRCGYC 3 cut(s) 1263, 1806, 1918
BstAUI TGTACA 2 cut(s) 2290, 3145
BstC8I GCNNGC 3 cut(s) 938, 1009, 2341
BstDEI CTNAG 9 cut(s) 289, 393, 1922, 2076, 2269, 2404, 2817, 3044, 3050
BstEII GGTNACC 1 cut(s) 1568
BstENI CCTNNNNNAGG 2 cut(s) 50, 2420
BstF5I GGATG 4 cut(s) 828, 973, 1494, 1570
BstFNI CGCG 1 cut(s) 2459
BstH2I RGCGCY 2 cut(s) 2346, 2444
BstHHI GCGC 2 cut(s) 2345, 2443
BstMAI GTCTC 3 cut(s) 1599, 2290, 2407
BstMCI CGRYCG 2 cut(s) 259, 851
BstMWI GCNNNNNNNGC 6 cut(s) 445, 550, 1991, 2270, 2920, 3071
BstNI CCWGG 6 cut(s) 666, 729, 898, 1486, 1530, 1811
BstNSI RCATGY 3 cut(s) 845, 940, 1332
BstPAI GACNNNNGTC 1 cut(s) 2379
BstPI GGTNACC 1 cut(s) 1568
BstSCI CCNGG 7 cut(s) 564, 664, 727, 896, 1484, 1528, 1809
BstSFI CTRYAG 1 cut(s) 951
BstUI CGCG 1 cut(s) 2459
BstV1I GCAGC 5 cut(s) 556, 1075, 1100, 2371, 2978
BstV2I GAAGAC 2 cut(s) 1134, 1853
BstX2I RGATCY 4 cut(s) 947, 1511, 1966, 2176
BstXI CCANNNNNNTGG 1 cut(s) 1274
BstYI RGATCY 4 cut(s) 947, 1511, 1966, 2176
Bsu15I ATCGAT 1 cut(s) 199
Bsu36I CCTNAGG 1 cut(s) 2817
BsuI GTATCC 1 cut(s) 3126
BsuRI GGCC 8 cut(s) 71, 278, 725, 754, 2030, 2311, 2339, 2594
BsuTUI ATCGAT 1 cut(s) 199
BtsCI GGATG 4 cut(s) 828, 973, 1494, 1570
BtsI GCAGTG 3 cut(s) 1938, 1980, 2930
BtsIMutI CAGTG 8 cut(s) 206, 1422, 1938, 1980, 2029, 2353, 2930, 3013
Cac8I GCNNGC 3 cut(s) 938, 1009, 2341
CaiI CAGNNNCTG 1 cut(s) 2299
CciI TCATGA 3 cut(s) 1396, 1893, 2872
CfoI GCGC 2 cut(s) 2345, 2443
Cfr10I RCCGGY 1 cut(s) 1245
ClaI ATCGAT 1 cut(s) 199
CseI GACGC 4 cut(s) 929, 1271, 1814, 2465
DdeI CTNAG 9 cut(s) 289, 393, 1922, 2076, 2269, 2404, 2817, 3044, 3050
DrdI GACNNNNNNGTC 2 cut(s) 290, 3054
DriI GACNNNNNGTC 1 cut(s) 3217
DseDI GACNNNNNNGTC 2 cut(s) 290, 3054
EaeI YGGCCR 2 cut(s) 752, 2592
Eam1104I CTCTTC 2 cut(s) 2415, 3092
Eam1105I GACNNNNNGTC 1 cut(s) 3217
EarI CTCTTC 2 cut(s) 2415, 3092
Ecl136II GAGCTC 1 cut(s) 439
Eco130I CCWWGG 1 cut(s) 2153
Eco24I GRGCYC 2 cut(s) 441, 2054
Eco47I GGWCC 8 cut(s) 34, 562, 629, 1505, 1777, 2381, 3102, 3219
Eco47III AGCGCT 1 cut(s) 2442
Eco53kI GAGCTC 1 cut(s) 439
Eco57I CTGAAG 3 cut(s) 1091, 1716, 2989
Eco81I CCTNAGG 1 cut(s) 2817
Eco88I CYCGRG 1 cut(s) 2574
Eco91I GGTNACC 1 cut(s) 1568
EcoICRI GAGCTC 1 cut(s) 439
EcoNI CCTNNNNNAGG 2 cut(s) 50, 2420
EcoO109I RGGNCCY 4 cut(s) 562, 724, 1505, 3219
EcoO65I GGTNACC 1 cut(s) 1568
EcoRII CCWGG 6 cut(s) 664, 727, 896, 1484, 1528, 1809
EcoT14I CCWWGG 1 cut(s) 2153
EcoT22I ATGCAT 1 cut(s) 78
EcoT38I GRGCYC 2 cut(s) 441, 2054
ErhI CCWWGG 1 cut(s) 2153
FalI AAGNNNNNCTT 2 cut(s) 1845, 1877
FaqI GGGAC 1 cut(s) 2387
FbaI TGATCA 1 cut(s) 2761
FblI GTMKAC 2 cut(s) 2066, 2635
Fnu4HI GCNGC 5 cut(s) 545, 1064, 1089, 2360, 2967
FokI GGATG 4 cut(s) 835, 960, 1501, 1577
FriOI GRGCYC 2 cut(s) 441, 2054
Fsp4HI GCNGC 5 cut(s) 545, 1064, 1089, 2360, 2967
GlaI GCGC 2 cut(s) 2344, 2442
GluI GCNGC 5 cut(s) 545, 1064, 1089, 2360, 2967
GsuI CTGGAG 3 cut(s) 162, 287, 750
HaeII RGCGCY 2 cut(s) 2346, 2444
HaeIII GGCC 8 cut(s) 71, 278, 725, 754, 2030, 2311, 2339, 2594
HapII CCGG 6 cut(s) 566, 820, 1246, 1562, 2920, 3083
HgaI GACGC 4 cut(s) 929, 1271, 1814, 2465
HhaI GCGC 2 cut(s) 2345, 2443
Hin1I GRCGYC 3 cut(s) 1263, 1806, 1918
Hin6I GCGC 2 cut(s) 2343, 2441
HinP1I GCGC 2 cut(s) 2343, 2441
HincII GTYRAC 3 cut(s) 1261, 2163, 2413
HindII GTYRAC 3 cut(s) 1261, 2163, 2413
HindIII AAGCTT 3 cut(s) 80, 479, 3243
HpaII CCGG 6 cut(s) 566, 820, 1246, 1562, 2920, 3083
HphI GGTGA 6 cut(s) 974, 1028, 1757, 1826, 2377, 3221
Hpy99I CGWCG 1 cut(s) 2738
HpyCH4III ACNGT 8 cut(s) 611, 955, 1317, 2125, 2258, 2301, 2738, 3184
HpyCH4IV ACGT 4 cut(s) 1093, 1440, 1918, 2959
HpyF10VI GCNNNNNNNGC 6 cut(s) 445, 550, 1991, 2270, 2920, 3071
HpyF3I CTNAG 9 cut(s) 289, 393, 1922, 2076, 2269, 2404, 2817, 3044, 3050
HpySE526I ACGT 4 cut(s) 1093, 1440, 1918, 2959
Hsp92I GRCGYC 3 cut(s) 1263, 1806, 1918
HspAI GCGC 2 cut(s) 2343, 2441
Kpn2I TCCGGA 1 cut(s) 1561
KpnI GGTACC 1 cut(s) 867
Ksp22I TGATCA 1 cut(s) 2761
Lsp1109I GCAGC 5 cut(s) 556, 1075, 1100, 2371, 2978
LweI GCATC 4 cut(s) 1513, 2003, 2887, 3022
MaeII ACGT 4 cut(s) 1093, 1440, 1918, 2959
MfeI CAATTG 1 cut(s) 2831
MflI RGATCY 4 cut(s) 947, 1511, 1966, 2176
MhlI GDGCHC 3 cut(s) 441, 1115, 2054
MlsI TGGCCA 2 cut(s) 754, 2594
MluI ACGCGT 1 cut(s) 2457
MluNI TGGCCA 2 cut(s) 754, 2594
MlyI GAGTC 6 cut(s) 511, 541, 1171, 1834, 1944, 2232
Mox20I TGGCCA 2 cut(s) 754, 2594
Mph1103I ATGCAT 1 cut(s) 78
MroI TCCGGA 1 cut(s) 1561
MroXI GAANNNNTTC 1 cut(s) 1672
MscI TGGCCA 2 cut(s) 754, 2594
MseI TTAA 5 cut(s) 356, 525, 537, 2640, 3186
MslI CAYNNNNRTG 5 cut(s) 962, 1215, 1272, 1785, 2600
Msp20I TGGCCA 2 cut(s) 754, 2594
MspA1I CMGCKG 1 cut(s) 2362
MspI CCGG 6 cut(s) 566, 820, 1246, 1562, 2920, 3083
MspR9I CCNGG 7 cut(s) 566, 666, 729, 898, 1486, 1530, 1811
MunI CAATTG 1 cut(s) 2831
Mva1269I GAATGC 2 cut(s) 2564, 2607
MvaI CCWGG 6 cut(s) 666, 729, 898, 1486, 1530, 1811
MvnI CGCG 1 cut(s) 2459
MwoI GCNNNNNNNGC 6 cut(s) 445, 550, 1991, 2270, 2920, 3071
NciI CCSGG 1 cut(s) 566
NheI GCTAGC 1 cut(s) 1007
NmeAIII GCCGAG 2 cut(s) 1024, 1848
NmuCI GTSAC 5 cut(s) 955, 1870, 1936, 1952, 3209
NsiI ATGCAT 1 cut(s) 78
NspI RCATGY 3 cut(s) 845, 940, 1332
PaeI GCATGC 1 cut(s) 940
PagI TCATGA 3 cut(s) 1396, 1893, 2872
PasI CCCWGGG 1 cut(s) 1810
PciI ACATGT 2 cut(s) 841, 1328
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PctI GAATGC 2 cut(s) 2564, 2607
PdmI GAANNNNTTC 1 cut(s) 1672
PfeI GAWTC 7 cut(s) 92, 574, 1207, 1842, 1890, 1911, 1976
PflMI CCANNNNNTGG 3 cut(s) 665, 2154, 3206
PfoI TCCNGGA 1 cut(s) 664
PkrI GCNGC 5 cut(s) 546, 1065, 1090, 2361, 2968
Ple19I CGATCG 2 cut(s) 259, 851
PleI GAGTC 6 cut(s) 511, 540, 1170, 1833, 1943, 2232
PpsI GAGTC 6 cut(s) 511, 540, 1170, 1833, 1943, 2232
PpuMI RGGWCCY 3 cut(s) 562, 1505, 3219
PscI ACATGT 2 cut(s) 841, 1328
PshAI GACNNNNGTC 1 cut(s) 2379
PshBI ATTAAT 1 cut(s) 525
PsiI TTATAA 2 cut(s) 321, 2508
Psp124BI GAGCTC 1 cut(s) 441
Psp5II RGGWCCY 3 cut(s) 562, 1505, 3219
Psp6I CCWGG 6 cut(s) 664, 727, 896, 1484, 1528, 1809
PspEI GGTNACC 1 cut(s) 1568
PspGI CCWGG 6 cut(s) 664, 727, 896, 1484, 1528, 1809
PspPPI RGGWCCY 3 cut(s) 562, 1505, 3219
PstNI CAGNNNCTG 1 cut(s) 2299
PsuI RGATCY 4 cut(s) 947, 1511, 1966, 2176
PvuI CGATCG 2 cut(s) 259, 851
PvuII CAGCTG 1 cut(s) 2362
RseI CAYNNNNRTG 5 cut(s) 962, 1215, 1272, 1785, 2600
SacI GAGCTC 1 cut(s) 441
SaqAI TTAA 5 cut(s) 356, 525, 537, 2640, 3186
SatI GCNGC 5 cut(s) 545, 1064, 1089, 2360, 2967
ScaI AGTACT 2 cut(s) 119, 799
SchI GAGTC 6 cut(s) 511, 541, 1171, 1834, 1944, 2232
ScrFI CCNGG 7 cut(s) 566, 666, 729, 898, 1486, 1530, 1811
SduI GDGCHC 3 cut(s) 441, 1115, 2054
SfaNI GCATC 4 cut(s) 1513, 2003, 2887, 3022
SfcI CTRYAG 1 cut(s) 951
SinI GGWCC 8 cut(s) 34, 562, 629, 1505, 1777, 2381, 3102, 3219
SmiMI CAYNNNNRTG 5 cut(s) 962, 1215, 1272, 1785, 2600
SmlI CTYRAG 2 cut(s) 83, 1837
SmoI CTYRAG 2 cut(s) 83, 1837
SpeI ACTAGT 1 cut(s) 1232
SphI GCATGC 1 cut(s) 940
SsiI CCGC 7 cut(s) 514, 2357, 2426, 2516, 2677, 2857, 3000
SstI GAGCTC 1 cut(s) 441
StyD4I CCNGG 7 cut(s) 564, 664, 727, 896, 1484, 1528, 1809
StyI CCWWGG 1 cut(s) 2153
TaaI ACNGT 8 cut(s) 611, 955, 1317, 2125, 2258, 2301, 2738, 3184
TaiI ACGT 4 cut(s) 1096, 1443, 1921, 2962
TaqI TCGA 9 cut(s) 5, 199, 255, 847, 851, 1303, 1961, 2236, 2326
TaqII GACCGA 1 cut(s) 3119
TatI WGTACW 6 cut(s) 117, 797, 1446, 2290, 3145, 3283
TfiI GAWTC 7 cut(s) 92, 574, 1207, 1842, 1890, 1911, 1976
Tru1I TTAA 5 cut(s) 356, 525, 537, 2640, 3186
Tru9I TTAA 5 cut(s) 356, 525, 537, 2640, 3186
TscAI CASTG 8 cut(s) 213, 1422, 1945, 1987, 2029, 2353, 2930, 3013
TseFI GTSAC 5 cut(s) 955, 1870, 1936, 1952, 3209
TseI GCWGC 5 cut(s) 544, 1063, 1088, 2359, 2966
Tsp45I GTSAC 5 cut(s) 955, 1870, 1936, 1952, 3209
TspGWI ACGGA 3 cut(s) 705, 1817, 2131
TspRI CASTG 8 cut(s) 213, 1422, 1945, 1987, 2029, 2353, 2930, 3013
Van91I CCANNNNNTGG 3 cut(s) 665, 2154, 3206
VpaK11BI GGWCC 8 cut(s) 34, 562, 629, 1505, 1777, 2381, 3102, 3219
VspI ATTAAT 1 cut(s) 525
XagI CCTNNNNNAGG 2 cut(s) 50, 2420
XapI RAATTY 6 cut(s) 7, 185, 674, 1201, 1668, 2328
XbaI TCTAGA 1 cut(s) 878
XceI RCATGY 3 cut(s) 845, 940, 1332
XcmI CCANNNNNNNNNTGG 3 cut(s) 747, 1074, 3161
XmiI GTMKAC 2 cut(s) 2066, 2635
XmnI GAANNNNTTC 1 cut(s) 1672
ZraI GACGTC 1 cut(s) 1919
ZrmI AGTACT 2 cut(s) 119, 799
Zsp2I ATGCAT 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.