AT5G34930

Arogenate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003175
Physical Location & Seq
Forward (+)
13233357 .. 13235763
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G34930.2

Sequence Viewer

Length: 1923 bp
ATGGCAGAGACACTCATCACCAAACCTCCTCTCTCCCTCTCATTTACCTCTCTTTCCTCAATGCTCCCTTCTCTCTCTCTCTCCACCGCCAACCGCCACCTCTCCGTCACCGACACCATCCCTCTTCCCAACTCCAACTCCAACGCCACCCCTCCTCTCCGTATCGCCATCATCGGATTCGGAAACTACGGCCAATTCCTTGCCGAAACCCTAATTTCTCAAGGCCACATTCTCTTCGCTCACTCCCGATCCGATCACTCCTCCGCCGCTCGCCGTCTCGGTGTCTCATACTTCACCGATCTTCACGATCTCTGCGAACGTCATCCTGACGTAGTCCTTCTCTGTACTTCAATCCTCTCCATAGAGAATATTCTCAAAACGTTGCCGTTTCAGAGACTCCGTCGCAACACTCTCTTCGTTGATGTTCTCTCCGTTAAAGAGTTTGCTAAAACTCTTCTCCTTCAATACTTACCTGAAGATTTCGATATTCTTTGTACACATCCAATGTTTGGTCCTCAGAGTGTGAGTTCAAATCATGGCTGGAGAGGATTAAGATTTGTGTATGATAAAGTTAGGATTGGGGAAGAGAGATTGAGAGTCTCAAGGTGTGAGAGTTTTCTTGAGATTTTTGTTAGAGAAGGATGTGAGATGGTGGAGATGAGTGTTACTGATCATGATAAGTTTGCTGCTGAATCACAGTTTATAACTCATACTCTTGGTAGGCTTTTGGGGATGTTGAAGTTGATATCGACGCCGATTAATACGAAAGGGTACGAGGCGTTGCTTGATTTAGCTGAGAATATTTGTGGGGATAGTTTTGATTTGTATTATGGGTTGTTTGTGTATAATAACAACTCTTTGGAGGTGTTAGAGAGGATTGATTTGGCTTTCGAGGCTTTGCGTAAGGAGCTTTTTAGTCGGCTTCACGGTGTTGTGAGGAAGCAGTCTTTTGAAGGTGAAGCAAAGAAAGTTCATGTTTTTCCAAATTGTGGTGAAAATGATGCTTCTTTGGATATGATGAGGTCAGAAGATGTTGTTGTGAAGTATGAATATAACTCCCAGGTGTCTGGTAGTGTTAATGACGGTTCGAGGCTCAAGATTGGTATCGTCGGGTTTGGAAATTTTGGACAGTTTCTAGGTAAAACCATGGTCAAGCAGGGTCACACTGTGTTAGCTTATTCCAGAAGTGACTACACTGATGAAGCAGCAAAGCTCGGTGTTTCGTATTTTTCAGATCTTGATGATCTATTTGAAGAGCATCCTGAAGTTATTATTCTCTGTACGTCAATCCTTTCGACTGAAAAAGTTCTCGAGTCACTACCGTTTCAGAGACTGAAGAGAAGCACACTTTTTGTGGATGTACTCTCAGTAAAAGAGTTCCCGAGGAATTTATTTCTTCAAACTCTCCCACAAGATTTTGATATTTTGTGCACGCATCCTATGTTTGGGCCAGAGAGTGGTAAAAATGGATGGAACAATCTTGCCTTTGTGTTTGATAAGGTTAGGATTGGAATGGATGATAGAAGAAAATCGAGGTGTAACAGTTTTCTTGATATTTTTGCCCGTGAAGGATGTCGTATGGTGGAGATGTCGTGTGCTGAACATGATTGGCATGCTGCTGGATCACAGTTTATCACACACACAGTGGGAAGGCTTCTGGAGAAGCTGAGCTTGGAATCTACTCCTATAGATACCAAAGGTTATGAGACATTGCTAAAACTGGTGGAGAATACTGCTGGTGACAGCTTTGATCTGTACTATGGACTATTTTTATACAATCCTAATGCAATGGAACAGCTTGAGAGGTTTCATGTGGCTTTTGAATCATTGAAGACACAGCTCTTTGGACGACTACATTCTCAACATTCTCATGAGCTAGCTAAATCATCTTCCCCAAAGACAACTAAGCTATTAACTAGCTAA

Protein Analysis

640

Amino Acids

72.3

Weight (kDa)

5.95

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F420_oxidored PF03807 76 - 165 1.9e-09 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 105 - 195 5.3e-08 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 265 - 337 2.9e-30 TYRAAT1-like, C-terminal domain
2-Hacid_dh_C PF02826 370 - 475 5.9e-09 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
F420_oxidored PF03807 388 - 477 2.8e-08 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_2 PF03446 388 - 476 1.8e-06 NAD binding domain of 6-phosphogluconate dehydrogenase
PDH_N PF02153 403 - 511 8.9e-10 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 575 - 646 2e-33 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 704
AccB7I CCANNNNNTGG 3 cut(s) 509, 989, 1457
AccBSI CCGCTC 1 cut(s) 269
AciI CCGC 4 cut(s) 87, 94, 264, 267
AclI AACGTT 1 cut(s) 380
AclWI GGATC 2 cut(s) 243, 1630
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 2 cut(s) 1120, 1387
AcuI CTGAAG 3 cut(s) 495, 1284, 1355
AcyI GRCGYC 1 cut(s) 752
AdeI CACNNNGTG 2 cut(s) 1168, 1645
AfaI GTAC 6 cut(s) 346, 496, 773, 1282, 1362, 1757
AfiI CCNNNNNNNGG 4 cut(s) 509, 989, 1445, 1457
AgsI TTSAA 9 cut(s) 351, 464, 531, 739, 953, 1253, 1400, 1823, 1831
AjnI CCWGG 1 cut(s) 1059
AjuI GAANNNNNNNTTGG 2 cut(s) 1655, 1687
Alw21I GWGCWC 1 cut(s) 1433
Alw26I GTCTC 7 cut(s) 2, 281, 289, 388, 604, 1324, 1700
Alw44I GTGCAC 1 cut(s) 1429
AlwI GGATC 2 cut(s) 243, 1630
AlwNI CAGNNNCTG 1 cut(s) 1333
Ama87I CYCGRG 2 cut(s) 1310, 1381
AoxI GGCC 3 cut(s) 190, 223, 1448
ApaLI GTGCAC 1 cut(s) 1429
ApeKI GCWGC 3 cut(s) 686, 1205, 1616
ApoI RAATTY 2 cut(s) 1120, 1387
AseI ATTAAT 1 cut(s) 759
Asp700I GAANNNNTTC 1 cut(s) 1305
AspS9I GGNCC 2 cut(s) 512, 1448
AsuHPI GGTGA 6 cut(s) 10, 100, 286, 968, 1004, 1751
AsuNHI GCTAGC 1 cut(s) 1876
AvaI CYCGRG 2 cut(s) 1310, 1381
AvaII GGWCC 1 cut(s) 512
BaeGI GKGCMC 1 cut(s) 1433
BbsI GAAGAC 1 cut(s) 1838
Bbv12I GWGCWC 1 cut(s) 1433
BbvI GCAGC 3 cut(s) 673, 1217, 1603
BccI CCATC 4 cut(s) 125, 176, 643, 1464
BceAI ACGGC 3 cut(s) 205, 258, 370
BciT130I CCWGG 1 cut(s) 1061
BclI TGATCA 1 cut(s) 670
BcoDI GTCTC 7 cut(s) 2, 281, 289, 388, 604, 1324, 1700
BfaI CTAG 3 cut(s) 1136, 1877, 1917
BfmI CTRYAG 1 cut(s) 1686
BglII AGATCT 1 cut(s) 1234
BisI GCNGC 4 cut(s) 267, 687, 1206, 1617
BlpI GCTNAGC 1 cut(s) 1667
BlsI GCNGC 4 cut(s) 268, 688, 1207, 1618
Bme1390I CCNGG 1 cut(s) 1061
Bme18I GGWCC 1 cut(s) 512
BmeT110I CYCGRG 2 cut(s) 1310, 1381
BmgT120I GGNCC 2 cut(s) 512, 1448
BmrFI CCNGG 1 cut(s) 1061
BmsI GCATC 3 cut(s) 991, 1267, 1444
BmtI GCTAGC 1 cut(s) 1880
BpiI GAAGAC 1 cut(s) 1838
BpmI CTGGAG 2 cut(s) 562, 1679
Bpu1102I GCTNAGC 1 cut(s) 1667
BpuEI CTTGAG 5 cut(s) 204, 586, 641, 1079, 1820
BsaBI GATNNNNATC 1 cut(s) 1103
BsaHI GRCGYC 1 cut(s) 752
BsaJI CCNNGG 3 cut(s) 1059, 1146, 1382
BsaXI ACNNNNNCTCC 6 cut(s) 10, 40, 122, 152, 1577, 1607
Bsc4I CCNNNNNNNGG 4 cut(s) 509, 989, 1445, 1457
Bse1I ACTGG 1 cut(s) 1725
Bse3DI GCAATG 2 cut(s) 1709, 1794
Bse8I GATNNNNATC 1 cut(s) 1103
BseBI CCWGG 1 cut(s) 1061
BseDI CCNNGG 3 cut(s) 1059, 1146, 1382
BseJI GATNNNNATC 1 cut(s) 1103
BseLI CCNNNNNNNGG 4 cut(s) 509, 989, 1445, 1457
BseMI GCAATG 2 cut(s) 1709, 1794
BseMII CTCAG 4 cut(s) 530, 786, 1380, 1658
BseNI ACTGG 1 cut(s) 1725
BseRI GAGGAG 3 cut(s) 18, 144, 250
BseSI GKGCMC 1 cut(s) 1433
BseXI GCAGC 3 cut(s) 673, 1217, 1603
BshFI GGCC 3 cut(s) 192, 225, 1450
BsiHKAI GWGCWC 1 cut(s) 1433
BsiHKCI CYCGRG 2 cut(s) 1310, 1381
BslI CCNNNNNNNGG 4 cut(s) 509, 989, 1445, 1457
BsmAI GTCTC 7 cut(s) 2, 281, 289, 388, 604, 1324, 1700
BsmBI CGTCTC 1 cut(s) 281
BsnI GGCC 3 cut(s) 192, 225, 1450
BsoBI CYCGRG 2 cut(s) 1310, 1381
Bsp1286I GDGCHC 1 cut(s) 1433
Bsp1407I TGTACA 1 cut(s) 494
Bsp143I GATC 9 cut(s) 248, 253, 298, 307, 670, 1234, 1243, 1622, 1750
Bsp1720I GCTNAGC 1 cut(s) 1667
Bsp19I CCATGG 1 cut(s) 1146
BspACI CCGC 4 cut(s) 87, 94, 264, 267
BspANI GGCC 3 cut(s) 192, 225, 1450
BspCNI CTCAG 4 cut(s) 529, 787, 1379, 1659
BspHI TCATGA 2 cut(s) 673, 1870
BspOI GCTAGC 1 cut(s) 1880
BspPI GGATC 2 cut(s) 243, 1630
BspQI GCTCTTC 1 cut(s) 1248
BsrBI CCGCTC 1 cut(s) 269
BsrDI GCAATG 2 cut(s) 1709, 1794
BsrGI TGTACA 1 cut(s) 494
BsrI ACTGG 1 cut(s) 1725
BssECI CCNNGG 3 cut(s) 1059, 1146, 1382
BssMI GATC 9 cut(s) 248, 253, 298, 307, 670, 1234, 1243, 1622, 1750
BssNI GRCGYC 1 cut(s) 752
BssT1I CCWWGG 1 cut(s) 1146
Bst2UI CCWGG 1 cut(s) 1061
Bst4CI ACNGT 9 cut(s) 699, 929, 1085, 1131, 1168, 1323, 1544, 1629, 1645
Bst6I CTCTTC 7 cut(s) 129, 239, 419, 459, 579, 1248, 1331
BstACI GRCGYC 1 cut(s) 752
BstAUI TGTACA 1 cut(s) 494
BstC8I GCNNGC 4 cut(s) 271, 1433, 1614, 1878
BstDEI CTNAG 5 cut(s) 516, 795, 1366, 1667, 1905
BstDSI CCRYGG 1 cut(s) 1146
BstKTI GATC 9 cut(s) 251, 256, 301, 310, 673, 1237, 1246, 1625, 1753
BstMAI GTCTC 7 cut(s) 2, 281, 289, 388, 604, 1324, 1700
BstMBI GATC 9 cut(s) 248, 253, 298, 307, 670, 1234, 1243, 1622, 1750
BstMWI GCNNNNNNNGC 2 cut(s) 893, 907
BstNI CCWGG 1 cut(s) 1061
BstNSI RCATGY 1 cut(s) 1616
BstSCI CCNGG 1 cut(s) 1059
BstSFI CTRYAG 1 cut(s) 1686
BstSLI GKGCMC 1 cut(s) 1433
BstV1I GCAGC 3 cut(s) 673, 1217, 1603
BstV2I GAAGAC 1 cut(s) 1838
BstX2I RGATCY 1 cut(s) 1234
BstXI CCANNNNNNTGG 1 cut(s) 1067
BstYI RGATCY 1 cut(s) 1234
BsuRI GGCC 3 cut(s) 192, 225, 1450
BtgI CCRYGG 1 cut(s) 1146
BtsIMutI CAGTG 3 cut(s) 1164, 1194, 1650
Cac8I GCNNGC 4 cut(s) 271, 1433, 1614, 1878
CaiI CAGNNNCTG 1 cut(s) 1333
CciI TCATGA 2 cut(s) 673, 1870
Cfr13I GGNCC 2 cut(s) 512, 1448
CseI GACGC 1 cut(s) 760
Csp6I GTAC 6 cut(s) 345, 495, 772, 1281, 1361, 1756
CviAII CATG 8 cut(s) 536, 674, 974, 1147, 1604, 1613, 1811, 1871
CviQI GTAC 6 cut(s) 345, 495, 772, 1281, 1361, 1756
DdeI CTNAG 5 cut(s) 516, 795, 1366, 1667, 1905
DpnI GATC 9 cut(s) 250, 255, 300, 309, 672, 1236, 1245, 1624, 1752
DpnII GATC 9 cut(s) 248, 253, 298, 307, 670, 1234, 1243, 1622, 1750
DraIII CACNNNGTG 2 cut(s) 1168, 1645
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 7 cut(s) 129, 239, 419, 459, 579, 1248, 1331
EarI CTCTTC 7 cut(s) 129, 239, 419, 459, 579, 1248, 1331
EciI GGCGGA 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 1146
Eco32I GATATC 1 cut(s) 747
Eco47I GGWCC 1 cut(s) 512
Eco57I CTGAAG 3 cut(s) 495, 1284, 1355
Eco88I CYCGRG 2 cut(s) 1310, 1381
EcoRII CCWGG 1 cut(s) 1059
EcoRV GATATC 1 cut(s) 747
EcoT14I CCWWGG 1 cut(s) 1146
ErhI CCWWGG 1 cut(s) 1146
Esp3I CGTCTC 1 cut(s) 281
FaeI CATG 8 cut(s) 539, 677, 977, 1150, 1607, 1616, 1814, 1874
FalI AAGNNNNNCTT 2 cut(s) 1655, 1687
FatI CATG 8 cut(s) 535, 673, 973, 1146, 1603, 1612, 1810, 1870
FbaI TGATCA 1 cut(s) 670
Fnu4HI GCNGC 4 cut(s) 267, 687, 1206, 1617
Fsp4HI GCNGC 4 cut(s) 267, 687, 1206, 1617
FspBI CTAG 3 cut(s) 1136, 1877, 1917
GluI GCNGC 4 cut(s) 267, 687, 1206, 1617
GsuI CTGGAG 2 cut(s) 562, 1679
HaeIII GGCC 3 cut(s) 192, 225, 1450
HgaI GACGC 1 cut(s) 760
Hin1I GRCGYC 1 cut(s) 752
Hin1II CATG 8 cut(s) 539, 677, 977, 1150, 1607, 1616, 1814, 1874
HinfI GANTC 7 cut(s) 177, 396, 597, 692, 1313, 1676, 1823
HphI GGTGA 6 cut(s) 10, 100, 286, 968, 1004, 1751
Hpy166II GTNNAC 2 cut(s) 497, 1431
Hpy188I TCNGA 8 cut(s) 176, 182, 253, 393, 519, 1027, 1234, 1329
Hpy8I GTNNAC 2 cut(s) 497, 1431
Hpy99I CGWCG 3 cut(s) 405, 754, 1112
HpyAV CCTTC 7 cut(s) 78, 347, 470, 632, 947, 1562, 1644
HpyCH4III ACNGT 9 cut(s) 699, 929, 1085, 1131, 1168, 1323, 1544, 1629, 1645
HpyCH4IV ACGT 4 cut(s) 319, 330, 380, 1283
HpyCH4V TGCA 2 cut(s) 1431, 1787
HpyF10VI GCNNNNNNNGC 2 cut(s) 893, 907
HpyF3I CTNAG 5 cut(s) 516, 795, 1366, 1667, 1905
HpySE526I ACGT 4 cut(s) 319, 330, 380, 1283
Hsp92I GRCGYC 1 cut(s) 752
Hsp92II CATG 8 cut(s) 539, 677, 977, 1150, 1607, 1616, 1814, 1874
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 9 cut(s) 248, 253, 298, 307, 670, 1234, 1243, 1622, 1750
LguI GCTCTTC 1 cut(s) 1248
LmnI GCTCC 2 cut(s) 69, 907
Lsp1109I GCAGC 3 cut(s) 673, 1217, 1603
LweI GCATC 3 cut(s) 991, 1267, 1444
MaeI CTAG 3 cut(s) 1136, 1877, 1917
MaeII ACGT 4 cut(s) 319, 330, 380, 1283
MaeIII GTNAC 7 cut(s) 106, 664, 1160, 1187, 1314, 1538, 1739
MalI GATC 9 cut(s) 250, 255, 300, 309, 672, 1236, 1245, 1624, 1752
MbiI CCGCTC 1 cut(s) 269
MboI GATC 9 cut(s) 248, 253, 298, 307, 670, 1234, 1243, 1622, 1750
MflI RGATCY 1 cut(s) 1234
MhlI GDGCHC 1 cut(s) 1433
MluCI AATT 5 cut(s) 194, 213, 985, 1120, 1387
MlyI GAGTC 3 cut(s) 390, 606, 1322
MmeI TCCRAC 2 cut(s) 159, 165
MroXI GAANNNNTTC 1 cut(s) 1305
MseI TTAA 5 cut(s) 435, 551, 759, 1077, 1913
MslI CAYNNNNRTG 1 cut(s) 1869
MspR9I CCNGG 1 cut(s) 1061
MvaI CCWGG 1 cut(s) 1061
MwoI GCNNNNNNNGC 2 cut(s) 893, 907
NcoI CCATGG 1 cut(s) 1146
NdeII GATC 9 cut(s) 248, 253, 298, 307, 670, 1234, 1243, 1622, 1750
NheI GCTAGC 1 cut(s) 1876
NlaIII CATG 8 cut(s) 539, 677, 977, 1150, 1607, 1616, 1814, 1874
NmuCI GTSAC 5 cut(s) 106, 1160, 1187, 1314, 1739
NspI RCATGY 1 cut(s) 1616
PaeI GCATGC 1 cut(s) 1616
PaeR7I CTCGAG 1 cut(s) 1310
PagI TCATGA 2 cut(s) 673, 1870
PciSI GCTCTTC 1 cut(s) 1248
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PdmI GAANNNNTTC 1 cut(s) 1305
PfeI GAWTC 4 cut(s) 177, 692, 1676, 1823
PflFI GACNNNGTC 2 cut(s) 332, 399
PflMI CCANNNNNTGG 3 cut(s) 509, 989, 1457
PkrI GCNGC 4 cut(s) 268, 688, 1207, 1618
PleI GAGTC 3 cut(s) 390, 605, 1321
PpsI GAGTC 3 cut(s) 390, 605, 1321
PshBI ATTAAT 1 cut(s) 759
PsiI TTATAA 1 cut(s) 704
Psp1406I AACGTT 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 1059
PspGI CCWGG 1 cut(s) 1059
PspPI GGNCC 2 cut(s) 512, 1448
PstNI CAGNNNCTG 1 cut(s) 1333
PsuI RGATCY 1 cut(s) 1234
PsyI GACNNNGTC 2 cut(s) 332, 399
RsaI GTAC 6 cut(s) 346, 496, 773, 1282, 1362, 1757
RsaNI GTAC 6 cut(s) 345, 495, 772, 1281, 1361, 1756
RseI CAYNNNNRTG 1 cut(s) 1869
SapI GCTCTTC 1 cut(s) 1248
SaqAI TTAA 5 cut(s) 435, 551, 759, 1077, 1913
SatI GCNGC 4 cut(s) 267, 687, 1206, 1617
Sau3AI GATC 9 cut(s) 248, 253, 298, 307, 670, 1234, 1243, 1622, 1750
Sau96I GGNCC 2 cut(s) 512, 1448
SchI GAGTC 3 cut(s) 390, 606, 1322
ScrFI CCNGG 1 cut(s) 1061
SduI GDGCHC 1 cut(s) 1433
SfaNI GCATC 3 cut(s) 991, 1267, 1444
SfcI CTRYAG 1 cut(s) 1686
Sfr274I CTCGAG 1 cut(s) 1310
SinI GGWCC 1 cut(s) 512
SlaI CTCGAG 1 cut(s) 1310
SmiMI CAYNNNNRTG 1 cut(s) 1869
SmlI CTYRAG 6 cut(s) 219, 601, 620, 1094, 1310, 1799
SmoI CTYRAG 6 cut(s) 219, 601, 620, 1094, 1310, 1799
SphI GCATGC 1 cut(s) 1616
Sse9I AATT 5 cut(s) 194, 213, 985, 1120, 1387
SsiI CCGC 4 cut(s) 87, 94, 264, 267
SspI AATATT 2 cut(s) 370, 802
SspMI CTAG 3 cut(s) 1136, 1877, 1917
StyD4I CCNGG 1 cut(s) 1059
StyI CCWWGG 1 cut(s) 1146
TaaI ACNGT 9 cut(s) 699, 929, 1085, 1131, 1168, 1323, 1544, 1629, 1645
TaiI ACGT 4 cut(s) 322, 333, 383, 1286
TaqI TCGA 7 cut(s) 483, 749, 891, 1088, 1295, 1311, 1532
TasI AATT 5 cut(s) 194, 213, 985, 1120, 1387
TatI WGTACW 4 cut(s) 344, 494, 1360, 1755
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 4 cut(s) 177, 692, 1676, 1823
Tru1I TTAA 5 cut(s) 435, 551, 759, 1077, 1913
Tru9I TTAA 5 cut(s) 435, 551, 759, 1077, 1913
TscAI CASTG 3 cut(s) 1171, 1201, 1650
TseFI GTSAC 5 cut(s) 106, 1160, 1187, 1314, 1739
TseI GCWGC 3 cut(s) 686, 1205, 1616
Tsp45I GTSAC 5 cut(s) 106, 1160, 1187, 1314, 1739
TspDTI ATGAA 4 cut(s) 962, 1062, 1215, 1799
TspGWI ACGGA 4 cut(s) 94, 149, 389, 421
TspRI CASTG 3 cut(s) 1171, 1201, 1650
Tth111I GACNNNGTC 2 cut(s) 332, 399
Van91I CCANNNNNTGG 3 cut(s) 509, 989, 1457
VneI GTGCAC 1 cut(s) 1429
VpaK11BI GGWCC 1 cut(s) 512
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 2 cut(s) 1120, 1387
XceI RCATGY 1 cut(s) 1616
XhoI CTCGAG 1 cut(s) 1310
XmnI GAANNNNTTC 1 cut(s) 1305
XspI CTAG 3 cut(s) 1136, 1877, 1917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.