RchiOBHm_Chr2g0128291

Arogenate dehydrogenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003175
Physical Location & Seq
Reverse (-)
43224688 .. 43227463
2776 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50008

Sequence Viewer

Length: 1410 bp
ATGCTGAAGATTGCCATCATCGGCTTTGGCAACTTGGGACAATCCCTTGCCAAGACCTTTGCTGACCTAGGCCACATGGTCCTTGCACATTCTAGATGTGACCAATCCAAAATGGCCCAAGACCTCGACATTTCATATTTCTCTGACCCGCAAGACCTCTGTGAACAGAATCCACAGGTCATTTTGCTATGCACCTCCATAATCTCCACAGAATCCGTCCTCAAATCTTTACCTTTCCAGCACCATAGGCTTGACACATTATTTGTTGACATGCTTCCTGTCAAAGAGTTTTCAAAAGCATTATTACTCAAGTTGTTGCCTAGCTATATAGATGTTCTTTGCACCAACCCAATGTTCGGAGTTGAGAGAGGCAAACATGAGTGGAATGGCATGTTTTTTGTGTACGAAAAGGTTCGAATTGGCTCTGACGGAGTGGGGATTTCTTTCTGTGATAACTTCTTAAACATGTTTGAGAAAGAAGGGTGTAGAATGGTGGAAGTAAGCTGTTCTGAGCAGGATAAGTATGTCGCAGGTGAGCAGTTTATTACCCACACAGTTGGGAGAGTCTTGGGGATGTTGACATTGGAATCAACTCCAATAAATACAAAAGGTTATGAGACATTGTTGGATTTGGTGGACAACACAGCTAGGGATAGTTTTGATTTATATTATGAGTTGTTCATGTACAACAAAAATGCATTTGAGATGTTTGAGAGGTTGGACTTAGCTTTTGACACTTTGAAGAAACAGCTTTTTGGTTATTTGAGTGATGTTGTGAGAAAGCAAGTATTTGATAATGCAGAGAAGGTAAAAATGGGGCACGAGGAGTACATGATGTCTAACCCATCCCACAATGGAGCTGCCTCGGAATCTTCTTCAAAAGATATGAGGTCGCATAACATTGCTCAGGTATCTAACAGTAAAGCACAGATAATATCTGACCACTTTGATGACAACTCAAGGCTCAAGATTGCAATTGTTGGTTTTGGGAACTTCGGTCAGTTTCTTGCTAAAACTATTATACGTCAAGGTCATATAGTTCTAGCCTATTCTCGGTCAGACTACTATGATGTGGCTCAAAAGTTGGGGGTAACTTACTGCTCTGATGTCGAGGAACTTTGCAAAGAGAATCCAGAAGTGATACTTTTTTGTACCTCTATTCTTTCAACTGAGAAAGTTCTCAGGTCATTTCCAGTTCAGAGGTTGATGAGGAATACTTTGTTTGTTGATGTTCTTTCAGTTAAAGAATTTCCAAAAAACTTGTTTCTTCAAAAGTTGCCATTGAATTTTGATATTCTCTGTACTCATCCTATGTTTGGACCAGAGAGTGGTAAAAATGGGTGGGAGGCTCTTCCTTTTGTTTATGATAAGGTCAGGGTGGGAAGTGACGAATCAAGGGTATCACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00400 ko01100 ko01110 ko01230 map00400 map01100 map01110 map01230
Pfam Domains
Protein Families
F420_oxidored PDH

Protein Analysis

469

Amino Acids

53.22

Weight (kDa)

5.53

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F420_oxidored PF03807 3 - 91 1.3e-08 NADP oxidoreductase coenzyme F420-dependent
2-Hacid_dh_C PF02826 3 - 89 4.7e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
TYRAAT1_C PF26213 189 - 262 1.6e-32 TYRAAT1-like, C-terminal domain
F420_oxidored PF03807 323 - 412 8.1e-09 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 337 - 445 1.8e-08 Prephenate dehydrogenase, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 521
Acc36I ACCTGC 1 cut(s) 521
AccB7I CCANNNNNTGG 1 cut(s) 1328
AciI CCGC 1 cut(s) 149
AcsI RAATTY 2 cut(s) 1247, 1285
AcuI CTGAAG 1 cut(s) 26
AfaI GTAC 5 cut(s) 404, 686, 830, 1153, 1303
AfiI CCNNNNNNNGG 4 cut(s) 356, 1053, 1316, 1328
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 6 cut(s) 294, 742, 879, 1167, 1271, 1285
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AluBI AGCT 6 cut(s) 324, 504, 647, 728, 751, 860
AluI AGCT 6 cut(s) 324, 504, 647, 728, 751, 860
Alw26I GTCTC 1 cut(s) 611
AoxI GGCC 2 cut(s) 70, 114
ApeKI GCWGC 1 cut(s) 860
ApoI RAATTY 2 cut(s) 1247, 1285
ArsI GACNNNNNNTTYG 4 cut(s) 245, 277, 713, 745
Asp700I GAANNNNTTC 1 cut(s) 411
AspA2I CCTAGG 1 cut(s) 67
AspS9I GGNCC 3 cut(s) 79, 115, 1319
AsuHPI GGTGA 1 cut(s) 545
AsuII TTCGAA 1 cut(s) 415
AvaII GGWCC 2 cut(s) 79, 1319
AvrII CCTAGG 1 cut(s) 67
BaeGI GKGCMC 1 cut(s) 822
BauI CACGAG 1 cut(s) 821
BbvI GCAGC 1 cut(s) 847
BccI CCATC 2 cut(s) 23, 853
BcoDI GTCTC 1 cut(s) 611
BfaI CTAG 5 cut(s) 68, 93, 321, 648, 1043
BfuAI ACCTGC 1 cut(s) 521
BisI GCNGC 1 cut(s) 861
BlnI CCTAGG 1 cut(s) 67
BlsI GCNGC 1 cut(s) 862
Bme18I GGWCC 2 cut(s) 79, 1319
BmgT120I GGNCC 3 cut(s) 79, 115, 1319
Bpu10I CCTNAGC 1 cut(s) 906
Bpu14I TTCGAA 1 cut(s) 415
BpuEI CTTGAG 3 cut(s) 293, 943, 950
BsaBI GATNNNNATC 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 67, 864
Bsc4I CCNNNNNNNGG 4 cut(s) 356, 1053, 1316, 1328
Bse1I ACTGG 1 cut(s) 1193
Bse3DI GCAATG 1 cut(s) 900
Bse8I GATNNNNATC 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 67, 864
BseGI GGATG 3 cut(s) 579, 845, 1306
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 4 cut(s) 356, 1053, 1316, 1328
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 4 cut(s) 501, 920, 1161, 1195
BseNI ACTGG 1 cut(s) 1193
BseRI GAGGAG 1 cut(s) 839
BseSI GKGCMC 1 cut(s) 822
BseXI GCAGC 1 cut(s) 847
BshFI GGCC 2 cut(s) 72, 116
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 4 cut(s) 356, 1053, 1316, 1328
BsmAI GTCTC 1 cut(s) 611
BsmFI GGGAC 1 cut(s) 51
BsnI GGCC 2 cut(s) 72, 116
Bsp119I TTCGAA 1 cut(s) 415
Bsp1286I GDGCHC 1 cut(s) 822
Bsp1407I TGTACA 1 cut(s) 684
BspACI CCGC 1 cut(s) 149
BspANI GGCC 2 cut(s) 72, 116
BspCNI CTCAG 4 cut(s) 502, 919, 1162, 1194
BspMI ACCTGC 1 cut(s) 521
BspQI GCTCTTC 1 cut(s) 1356
BspT104I TTCGAA 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 900
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 1 cut(s) 1193
BssECI CCNNGG 2 cut(s) 67, 864
BssSI CACGAG 1 cut(s) 821
BssT1I CCWWGG 1 cut(s) 67
Bst2BI CACGAG 1 cut(s) 821
Bst4CI ACNGT 2 cut(s) 556, 920
Bst6I CTCTTC 1 cut(s) 1356
BstAUI TGTACA 1 cut(s) 684
BstBI TTCGAA 1 cut(s) 415
BstDEI CTNAG 5 cut(s) 510, 724, 906, 1170, 1181
BstF5I GGATG 3 cut(s) 579, 845, 1306
BstMAI GTCTC 1 cut(s) 611
BstMWI GCNNNNNNNGC 1 cut(s) 247
BstNSI RCATGY 3 cut(s) 274, 394, 469
BstSLI GKGCMC 1 cut(s) 822
BstV1I GCAGC 1 cut(s) 847
BstXI CCANNNNNNTGG 1 cut(s) 557
BsuRI GGCC 2 cut(s) 72, 116
BtsCI GGATG 3 cut(s) 579, 845, 1306
BveI ACCTGC 1 cut(s) 521
Cfr13I GGNCC 3 cut(s) 79, 115, 1319
Csp6I GTAC 5 cut(s) 403, 685, 829, 1152, 1302
CviAII CATG 7 cut(s) 76, 271, 377, 391, 466, 682, 832
CviQI GTAC 5 cut(s) 403, 685, 829, 1152, 1302
DdeI CTNAG 5 cut(s) 510, 724, 906, 1170, 1181
Eam1104I CTCTTC 1 cut(s) 1356
EarI CTCTTC 1 cut(s) 1356
Eco130I CCWWGG 1 cut(s) 67
Eco47I GGWCC 2 cut(s) 79, 1319
Eco57I CTGAAG 1 cut(s) 26
EcoT14I CCWWGG 1 cut(s) 67
EcoT22I ATGCAT 1 cut(s) 700
ErhI CCWWGG 1 cut(s) 67
FaeI CATG 7 cut(s) 79, 274, 380, 394, 469, 685, 835
FalI AAGNNNNNCTT 2 cut(s) 1128, 1160
FaqI GGGAC 1 cut(s) 51
FatI CATG 7 cut(s) 75, 270, 376, 390, 465, 681, 831
FauI CCCGC 1 cut(s) 156
Fnu4HI GCNGC 1 cut(s) 861
FokI GGATG 3 cut(s) 586, 832, 1293
Fsp4HI GCNGC 1 cut(s) 861
FspBI CTAG 5 cut(s) 68, 93, 321, 648, 1043
GluI GCNGC 1 cut(s) 861
HaeIII GGCC 2 cut(s) 72, 116
Hin1II CATG 7 cut(s) 79, 274, 380, 394, 469, 685, 835
HincII GTYRAC 2 cut(s) 268, 579
HindII GTYRAC 2 cut(s) 268, 579
HinfI GANTC 7 cut(s) 169, 212, 564, 587, 869, 1129, 1391
HphI GGTGA 1 cut(s) 545
Hpy166II GTNNAC 5 cut(s) 164, 268, 403, 579, 637
Hpy188III TCNNGA 3 cut(s) 93, 967, 1133
Hpy8I GTNNAC 5 cut(s) 164, 268, 403, 579, 637
HpyAV CCTTC 2 cut(s) 473, 799
HpyCH4III ACNGT 2 cut(s) 556, 920
HpyCH4IV ACGT 1 cut(s) 1024
HpyCH4V TGCA 7 cut(s) 86, 192, 342, 698, 800, 974, 1122
HpyF10VI GCNNNNNNNGC 1 cut(s) 247
HpyF3I CTNAG 5 cut(s) 510, 724, 906, 1170, 1181
HpySE526I ACGT 1 cut(s) 1024
Hsp92II CATG 7 cut(s) 79, 274, 380, 394, 469, 685, 835
LguI GCTCTTC 1 cut(s) 1356
LmnI GCTCC 1 cut(s) 857
Lsp1109I GCAGC 1 cut(s) 847
MaeI CTAG 5 cut(s) 68, 93, 321, 648, 1043
MaeII ACGT 1 cut(s) 1024
MaeIII GTNAC 3 cut(s) 98, 1090, 1385
MboII GAAGA 6 cut(s) 19, 754, 864, 867, 1259, 1343
MfeI CAATTG 1 cut(s) 975
MhlI GDGCHC 1 cut(s) 822
MluCI AATT 4 cut(s) 417, 975, 1247, 1285
MlyI GAGTC 1 cut(s) 573
MmeI TCCRAC 2 cut(s) 606, 699
Mph1103I ATGCAT 1 cut(s) 700
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 2 cut(s) 461, 1242
MslI CAYNNNNRTG 1 cut(s) 948
MunI CAATTG 1 cut(s) 975
MwoI GCNNNNNNNGC 1 cut(s) 247
NlaIII CATG 7 cut(s) 79, 274, 380, 394, 469, 685, 835
NmuCI GTSAC 2 cut(s) 98, 1385
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 3 cut(s) 274, 394, 469
NspV TTCGAA 1 cut(s) 415
PaqCI CACCTGC 1 cut(s) 521
PciI ACATGT 1 cut(s) 465
PciSI GCTCTTC 1 cut(s) 1356
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 6 cut(s) 169, 212, 587, 869, 1129, 1391
PflMI CCANNNNNTGG 1 cut(s) 1328
PkrI GCNGC 1 cut(s) 862
PleI GAGTC 1 cut(s) 572
PpsI GAGTC 1 cut(s) 572
PscI ACATGT 1 cut(s) 465
PspPI GGNCC 3 cut(s) 79, 115, 1319
RsaI GTAC 5 cut(s) 404, 686, 830, 1153, 1303
RsaNI GTAC 5 cut(s) 403, 685, 829, 1152, 1302
RseI CAYNNNNRTG 1 cut(s) 948
SapI GCTCTTC 1 cut(s) 1356
SaqAI TTAA 2 cut(s) 461, 1242
SatI GCNGC 1 cut(s) 861
Sau96I GGNCC 3 cut(s) 79, 115, 1319
SchI GAGTC 1 cut(s) 573
SduI GDGCHC 1 cut(s) 822
SfuI TTCGAA 1 cut(s) 415
SinI GGWCC 2 cut(s) 79, 1319
SmiMI CAYNNNNRTG 1 cut(s) 948
SmlI CTYRAG 3 cut(s) 308, 958, 965
SmoI CTYRAG 3 cut(s) 308, 958, 965
Sse9I AATT 4 cut(s) 417, 975, 1247, 1285
SsiI CCGC 1 cut(s) 149
SspMI CTAG 5 cut(s) 68, 93, 321, 648, 1043
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 2 cut(s) 556, 920
TaiI ACGT 1 cut(s) 1027
TaqI TCGA 3 cut(s) 126, 415, 1110
TaqII GACCGA 2 cut(s) 986, 1044
TasI AATT 4 cut(s) 417, 975, 1247, 1285
TatI WGTACW 3 cut(s) 684, 828, 1301
TfiI GAWTC 6 cut(s) 169, 212, 587, 869, 1129, 1391
Tru1I TTAA 2 cut(s) 461, 1242
Tru9I TTAA 2 cut(s) 461, 1242
TseFI GTSAC 2 cut(s) 98, 1385
TseI GCWGC 1 cut(s) 860
Tsp45I GTSAC 2 cut(s) 98, 1385
TspDTI ATGAA 2 cut(s) 123, 670
TspGWI ACGGA 2 cut(s) 205, 444
Van91I CCANNNNNTGG 1 cut(s) 1328
VpaK11BI GGWCC 2 cut(s) 79, 1319
XapI RAATTY 2 cut(s) 1247, 1285
XbaI TCTAGA 1 cut(s) 92
XceI RCATGY 3 cut(s) 274, 394, 469
XmaJI CCTAGG 1 cut(s) 67
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 5 cut(s) 68, 93, 321, 648, 1043
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.