Rh2BG026500

Arogenate dehydrogenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003175
Physical Location & Seq
Reverse (-)
1760439 .. 1804156
43718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG026500.1

Sequence Viewer

Length: 2076 bp
ATGCTATCCCTCTCACCTCTCCACCCATTGCCTCCAAAACTCATCTCCACTCGCTCTCCCTTCTTCAACCCCCAAAGCCCCAACCTTTACCCCCAAAGCTCCAACCTTTCCCCCAAAATCCCTAACCTTTCCCCCAAAATCCCTAACCTTTCCCCAAAACGACGCCGTTCCCTCAAAATCAAATCCCTAGACGCCGCCCAATTCTTCGACTACGAAACCAAGCTCGCCGCCCAGTACCACTCCTCCCAAACCCTCAAGATCGCCATCATCGGCTTCGGCAACTTCGGCCAATTCCTCGCCAAAACCCTAACCCGCCAAGGCCACACCGTCCTCGCCTATTCCCGCTCCGACCACTCCAAAACGGCAGCCGCCCTCGGCGTTTCCTTCTTCTCCGACCCCCACGACCTCTGCGAGCAGCACCCCCACGTCATCCTCCTCTGCACCTCCATCATCTCCGCCGAGAAAGTCCTCAACTCCCTCCCCCTCCAGCGCCTCCGCCGCAACACTCTCTTCGCCGACGTCCTCTCCGTCAAGGAGTTCTCCAAAGCTCTGTTCTTGAAATCTCTTCCGAGCTATTTCGATGTGATCTGCACGCACCCGATGTTCGGGCCGGAGAGCGCCAAGAACGGCTGGAGTGGGCTGTGCTTTGTTTACGAGAAAGTGAGGATTGGATCGGAGGTGTCTAGAGTTTCGAGGTGTGACAAGTTCTTGAGCATTTTCGAGAGGGAAGGGTGTGAAATGGTGGAAATGAGCTGTGCCGAGCATGATAAGTTCGCGGCGGAGTCGCAGTTTATTACACACACAGTTGGGAGGGTGCTTGGGATGTTGAGGTTGGAGTCGACGCCGATTAATACTAGAGGGTATGAGACATTGTTGGATTTGGTGGAGAATACGGCTGGGGATAGCTTTGATTTGTATTATGGGTTGTTTATGTATAATAAGAATGCGTTGGAGATGCTGGAGAGGTTGGACTTGGCTTTCGAGGATTTGAAGAAGCAGCTTTTCGGGAGGTTGCATGATGTTGTGAGGAACCAGTTGTTTGGGAATGCTGAAAAGGTGAGGACGTTGCAGGAGGAGTATGTGAATCAGAATGGAGCTGCATTGGTGCGTTCTTCAAAAGCTGTGAGATCCCCTAAAGTAGATATTTTGGATAGCTCTGAAGACCATGCAAGGCTAAAGATTGGAATTGTTGGTTTTGGCAACTTTGGTCAGTTCCTTGCTAAAACCATTGTACGCCAAGGCCACAAAGTTCTAGCCTATTCTCGGTCAGACTACTCTGATGTTGCTCAACAGTTGGGTGTTTCTTACTTCTCGGATGCAGATGATTTTTGCGAGGAGCATCCAGAAGTAATACTCCTTTGCACATCGATTCTTTCCACCGAGAAAGTTCTGATGTCACTCCCGCTTCAGAGGTTGAAGCGAAGTACTTTGTTTGTTGATGTTCTATCCGTGAAAGAAATCCCAAGAAGCCTGTTTCTTCAGAATTTGCCTCTAGATTTTGATATTCTCTGCACACATCCTATGTTTGGACCAGAAAGCGGTAAAAATGGGTGGAATGGTCTTGCTTTTGTTTATGATAGGGTCAGAATTGGAAATGACGAATCAAGGGTGTCAAGGTGTGATCAATTTCTTGATATTTTTGCGCAAGAAGGGTGTCGAATGGTGGATATGTCTTGCGAAGAGCATGATAAGCATGCAGCAGGGTCACAATTCATCACACACACTATGGGGAGAGTTTTGGAAAAGTTGGGTTTGGAGTCGACACCGATCAATACAAAAGGTTACAAGACTTTGTTGAATTTGGTGGAAAATACAGCAGGAGATAGCTTTGATCTCTACTATGGATTGTTCATGTACAATGTAAATGCAATGGAGCAGCTAAAGAGGCTGGACCTTGCTTTTGAAGCATTAAAGAATCAGCTTTTTGGGCGGTTGCATGGTGTCCTTCGGAAGCAACTTTTTGAGAATGCAGATGATTCTCAAGTTATGAAGGAGCAGCACTTGTTGCCAACACCATCGGAGATTGAATCTGCAGCGACATCTTCTTTCGAGGCTCTTAACATTCAGAACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00400 ko01100 ko01110 ko01230 map00400 map01100 map01110 map01230
Pfam Domains
Protein Families
F420_oxidored PDH

Protein Analysis

691

Amino Acids

77.84

Weight (kDa)

6.59

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 72 - 171 2.4e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
F420_oxidored PF03807 86 - 170 2e-09 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_2 PF03446 86 - 173 8.9e-07 NAD binding domain of 6-phosphogluconate dehydrogenase
PDH_N PF02153 99 - 206 7.4e-09 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 272 - 345 2e-35 TYRAAT1-like, C-terminal domain
2-Hacid_dh_C PF02826 385 - 480 5.8e-08 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
F420_oxidored PF03807 393 - 482 7e-10 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_2 PF03446 393 - 481 5.1e-06 NAD binding domain of 6-phosphogluconate dehydrogenase
PDH_N PF02153 407 - 515 3.6e-09 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 579 - 652 2e-35 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 522
Acc16I TGCGCA 1 cut(s) 1644
AccBSI CCGCTC 1 cut(s) 345
AccI GTMKAC 2 cut(s) 839, 1760
AccII CGCG 1 cut(s) 776
AclWI GGATC 2 cut(s) 679, 1122
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 2 cut(s) 1483, 1798
AcuI CTGAAG 3 cut(s) 1179, 1391, 1463
AcyI GRCGYC 4 cut(s) 163, 192, 519, 842
AfaI GTAC 4 cut(s) 236, 1233, 1426, 1856
AfiI CCNNNNNNNGG 4 cut(s) 605, 1263, 1526, 1538
AgsI TTSAA 8 cut(s) 67, 559, 991, 1116, 1417, 1798, 1904, 2027
AjiI CACGTC 1 cut(s) 427
AjuI GAANNNNNNNTTGG 2 cut(s) 536, 568
Alw26I GTCTC 1 cut(s) 860
AlwI GGATC 2 cut(s) 679, 1122
AoxI GGCC 4 cut(s) 286, 319, 608, 1240
ApeKI GCWGC 8 cut(s) 365, 415, 997, 1097, 1697, 1876, 1996, 2033
ApoI RAATTY 2 cut(s) 1483, 1798
ArsI GACNNNNNNTTYG 4 cut(s) 962, 994, 1883, 1915
AseI ATTAAT 1 cut(s) 849
AspLEI GCGC 3 cut(s) 492, 620, 1645
AspS9I GGNCC 3 cut(s) 608, 1529, 1891
AsuHPI GGTGA 2 cut(s) 6, 1069
AvaII GGWCC 2 cut(s) 1529, 1891
BbsI GAAGAC 1 cut(s) 1167
BbvI GCAGC 8 cut(s) 377, 427, 1009, 1084, 1709, 1888, 2008, 2045
BccI CCATC 3 cut(s) 272, 455, 2023
BceAI ACGGC 4 cut(s) 150, 378, 643, 909
BclI TGATCA 1 cut(s) 1621
BcoDI GTCTC 1 cut(s) 860
BfaI CTAG 5 cut(s) 188, 684, 855, 1253, 1493
BfmI CTRYAG 1 cut(s) 2031
BfoI RGCGCY 2 cut(s) 493, 621
BmcAI AGTACT 1 cut(s) 1426
Bme18I GGWCC 2 cut(s) 1529, 1891
BmgBI CACGTC 1 cut(s) 427
BmgT120I GGNCC 3 cut(s) 608, 1529, 1891
BmiI GGNNCC 1 cut(s) 1031
BmrI ACTGGG 1 cut(s) 226
BmsI GCATC 3 cut(s) 945, 1306, 1348
BmuI ACTGGG 1 cut(s) 226
BpiI GAAGAC 1 cut(s) 1167
BpmI CTGGAG 3 cut(s) 470, 652, 980
BpuEI CTTGAG 3 cut(s) 239, 730, 1965
Bsa29I ATCGAT 1 cut(s) 1367
BsaBI GATNNNNATC 1 cut(s) 263
BsaHI GRCGYC 4 cut(s) 163, 192, 519, 842
BsaJI CCNNGG 3 cut(s) 316, 373, 1237
BsaXI ACNNNNNCTCC 8 cut(s) 40, 70, 227, 257, 509, 539, 773, 803
Bsc4I CCNNNNNNNGG 4 cut(s) 605, 1263, 1526, 1538
Bse1I ACTGG 2 cut(s) 232, 1033
Bse3DI GCAATG 2 cut(s) 26, 1875
Bse8I GATNNNNATC 1 cut(s) 263
BseCI ATCGAT 1 cut(s) 1367
BseDI CCNNGG 3 cut(s) 316, 373, 1237
BseGI GGATG 5 cut(s) 429, 828, 1321, 1339, 1516
BseJI GATNNNNATC 1 cut(s) 263
BseLI CCNNNNNNNGG 4 cut(s) 605, 1263, 1526, 1538
BseMI GCAATG 2 cut(s) 26, 1875
BseNI ACTGG 2 cut(s) 232, 1033
BseRI GAGGAG 4 cut(s) 232, 425, 1088, 1349
BseXI GCAGC 8 cut(s) 377, 427, 1009, 1084, 1709, 1888, 2008, 2045
BseYI CCCAGC 1 cut(s) 896
BsgI GTGCAG 3 cut(s) 424, 574, 1495
Bsh1236I CGCG 1 cut(s) 776
BshFI GGCC 4 cut(s) 288, 321, 610, 1242
BshVI ATCGAT 1 cut(s) 1367
BsiSI CCGG 1 cut(s) 611
BslI CCNNNNNNNGG 4 cut(s) 605, 1263, 1526, 1538
BsmAI GTCTC 1 cut(s) 860
BsmI GAATGC 3 cut(s) 949, 1051, 1972
BsnI GGCC 4 cut(s) 288, 321, 610, 1242
Bsp1407I TGTACA 1 cut(s) 1854
Bsp143I GATC 7 cut(s) 258, 585, 671, 1127, 1621, 1767, 1831
BspANI GGCC 4 cut(s) 288, 321, 610, 1242
BspDI ATCGAT 1 cut(s) 1367
BspFNI CGCG 1 cut(s) 776
BspLI GGNNCC 1 cut(s) 1031
BspMAI CTGCAG 1 cut(s) 2035
BspPI GGATC 2 cut(s) 679, 1122
BspQI GCTCTTC 1 cut(s) 1674
BsrBI CCGCTC 1 cut(s) 345
BsrDI GCAATG 2 cut(s) 26, 1875
BsrGI TGTACA 1 cut(s) 1854
BsrI ACTGG 2 cut(s) 232, 1033
BssECI CCNNGG 3 cut(s) 316, 373, 1237
BssMI GATC 7 cut(s) 258, 585, 671, 1127, 1621, 1767, 1831
BssNI GRCGYC 4 cut(s) 163, 192, 519, 842
BssT1I CCWWGG 2 cut(s) 316, 1237
Bst4CI ACNGT 3 cut(s) 328, 805, 1293
Bst6I CTCTTC 3 cut(s) 515, 570, 1674
BstACI GRCGYC 4 cut(s) 163, 192, 519, 842
BstAPI GCANNNNNTGC 1 cut(s) 2005
BstAUI TGTACA 1 cut(s) 1854
BstC8I GCNNGC 4 cut(s) 225, 413, 593, 1695
BstF5I GGATG 5 cut(s) 429, 828, 1321, 1339, 1516
BstFNI CGCG 1 cut(s) 776
BstH2I RGCGCY 2 cut(s) 493, 621
BstHHI GCGC 3 cut(s) 492, 620, 1645
BstKTI GATC 7 cut(s) 261, 588, 674, 1130, 1624, 1770, 1834
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 7 cut(s) 258, 585, 671, 1127, 1621, 1767, 1831
BstMWI GCNNNNNNNGC 7 cut(s) 285, 498, 1690, 1885, 1904, 1927, 2005
BstNSI RCATGY 1 cut(s) 1697
BstSFI CTRYAG 1 cut(s) 2031
BstUI CGCG 1 cut(s) 776
BstV1I GCAGC 8 cut(s) 377, 427, 1009, 1084, 1709, 1888, 2008, 2045
BstV2I GAAGAC 1 cut(s) 1167
BstX2I RGATCY 1 cut(s) 1127
BstXI CCANNNNNNTGG 1 cut(s) 1040
BstYI RGATCY 1 cut(s) 1127
Bsu15I ATCGAT 1 cut(s) 1367
BsuRI GGCC 4 cut(s) 288, 321, 610, 1242
BsuTUI ATCGAT 1 cut(s) 1367
BtrI CACGTC 1 cut(s) 427
BtsCI GGATG 5 cut(s) 429, 828, 1321, 1339, 1516
Cac8I GCNNGC 4 cut(s) 225, 413, 593, 1695
CfoI GCGC 3 cut(s) 492, 620, 1645
Cfr13I GGNCC 3 cut(s) 608, 1529, 1891
ClaI ATCGAT 1 cut(s) 1367
CseI GACGC 3 cut(s) 171, 200, 850
Csp6I GTAC 4 cut(s) 235, 1232, 1425, 1855
CviAII CATG 7 cut(s) 764, 1016, 1166, 1685, 1694, 1852, 1937
CviQI GTAC 4 cut(s) 235, 1232, 1425, 1855
DpnI GATC 7 cut(s) 260, 587, 673, 1129, 1623, 1769, 1833
DpnII GATC 7 cut(s) 258, 585, 671, 1127, 1621, 1767, 1831
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 3 cut(s) 515, 570, 1674
EarI CTCTTC 3 cut(s) 515, 570, 1674
EciI GGCGGA 3 cut(s) 445, 485, 794
Eco130I CCWWGG 2 cut(s) 316, 1237
Eco47I GGWCC 2 cut(s) 1529, 1891
Eco57I CTGAAG 3 cut(s) 1179, 1391, 1463
EcoT14I CCWWGG 2 cut(s) 316, 1237
ErhI CCWWGG 2 cut(s) 316, 1237
FaeI CATG 7 cut(s) 767, 1019, 1169, 1688, 1697, 1855, 1940
FatI CATG 7 cut(s) 763, 1015, 1165, 1684, 1693, 1851, 1936
FauI CCCGC 3 cut(s) 320, 350, 1410
FbaI TGATCA 1 cut(s) 1621
FblI GTMKAC 2 cut(s) 839, 1760
FokI GGATG 5 cut(s) 416, 835, 1326, 1328, 1503
FspBI CTAG 5 cut(s) 188, 684, 855, 1253, 1493
FspI TGCGCA 1 cut(s) 1644
GlaI GCGC 3 cut(s) 491, 619, 1644
GsaI CCCAGC 1 cut(s) 900
GsuI CTGGAG 3 cut(s) 470, 652, 980
HaeII RGCGCY 2 cut(s) 493, 621
HaeIII GGCC 4 cut(s) 288, 321, 610, 1242
HapII CCGG 1 cut(s) 611
HgaI GACGC 3 cut(s) 171, 200, 850
HhaI GCGC 3 cut(s) 492, 620, 1645
Hin1I GRCGYC 4 cut(s) 163, 192, 519, 842
Hin1II CATG 7 cut(s) 767, 1019, 1169, 1688, 1697, 1855, 1940
Hin6I GCGC 3 cut(s) 490, 618, 1643
HinP1I GCGC 3 cut(s) 490, 618, 1643
HincII GTYRAC 2 cut(s) 840, 1761
HindII GTYRAC 2 cut(s) 840, 1761
HinfI GANTC 9 cut(s) 782, 836, 1084, 1369, 1601, 1757, 1915, 1976, 2027
HpaII CCGG 1 cut(s) 611
HphI GGTGA 2 cut(s) 6, 1069
Hpy166II GTNNAC 3 cut(s) 652, 840, 1761
Hpy188III TCNNGA 9 cut(s) 256, 556, 684, 709, 721, 1006, 1343, 1493, 1631
Hpy8I GTNNAC 3 cut(s) 652, 840, 1761
Hpy99I CGWCG 3 cut(s) 165, 521, 844
HpyAV CCTTC 6 cut(s) 70, 394, 722, 1643, 1955, 1984
HpyCH4III ACNGT 3 cut(s) 328, 805, 1293
HpyCH4IV ACGT 3 cut(s) 426, 519, 1064
HpyF10VI GCNNNNNNNGC 7 cut(s) 285, 498, 1690, 1885, 1904, 1927, 2005
HpySE526I ACGT 3 cut(s) 426, 519, 1064
Hsp92I GRCGYC 4 cut(s) 163, 192, 519, 842
Hsp92II CATG 7 cut(s) 767, 1019, 1169, 1688, 1697, 1855, 1940
HspAI GCGC 3 cut(s) 490, 618, 1643
Ksp22I TGATCA 1 cut(s) 1621
Kzo9I GATC 7 cut(s) 258, 585, 671, 1127, 1621, 1767, 1831
LguI GCTCTTC 1 cut(s) 1674
LmnI GCTCC 6 cut(s) 104, 350, 1094, 1336, 1873, 1993
Lsp1109I GCAGC 8 cut(s) 377, 427, 1009, 1084, 1709, 1888, 2008, 2045
LweI GCATC 3 cut(s) 945, 1306, 1348
MaeI CTAG 5 cut(s) 188, 684, 855, 1253, 1493
MaeII ACGT 3 cut(s) 426, 519, 1064
MaeIII GTNAC 4 cut(s) 698, 1395, 1704, 1781
MalI GATC 7 cut(s) 260, 587, 673, 1129, 1623, 1769, 1833
MbiI CCGCTC 1 cut(s) 345
MboI GATC 7 cut(s) 258, 585, 671, 1127, 1621, 1767, 1831
MflI RGATCY 1 cut(s) 1127
MluCI AATT 8 cut(s) 200, 290, 1185, 1483, 1587, 1625, 1709, 1798
MlyI GAGTC 3 cut(s) 791, 845, 1766
MmeI TCCRAC 7 cut(s) 126, 372, 417, 813, 855, 930, 948
MseI TTAA 3 cut(s) 849, 1910, 2058
MspI CCGG 1 cut(s) 611
Mva1269I GAATGC 3 cut(s) 949, 1051, 1972
MvnI CGCG 1 cut(s) 776
MwoI GCNNNNNNNGC 7 cut(s) 285, 498, 1690, 1885, 1904, 1927, 2005
NdeII GATC 7 cut(s) 258, 585, 671, 1127, 1621, 1767, 1831
NlaIII CATG 7 cut(s) 767, 1019, 1169, 1688, 1697, 1855, 1940
NlaIV GGNNCC 1 cut(s) 1031
NmeAIII GCCGAG 3 cut(s) 354, 484, 784
NmuCI GTSAC 3 cut(s) 698, 1395, 1704
NsbI TGCGCA 1 cut(s) 1644
NspI RCATGY 1 cut(s) 1697
PaeI GCATGC 1 cut(s) 1697
PciSI GCTCTTC 1 cut(s) 1674
PcsI WCGNNNNNNNCGW 2 cut(s) 408, 525
PctI GAATGC 3 cut(s) 949, 1051, 1972
PfeI GAWTC 6 cut(s) 1084, 1369, 1601, 1915, 1976, 2027
PleI GAGTC 3 cut(s) 790, 844, 1765
PpsI GAGTC 3 cut(s) 790, 844, 1765
PshBI ATTAAT 1 cut(s) 849
PspFI CCCAGC 1 cut(s) 896
PspN4I GGNNCC 1 cut(s) 1031
PspPI GGNCC 3 cut(s) 608, 1529, 1891
PstI CTGCAG 1 cut(s) 2035
PsuI RGATCY 1 cut(s) 1127
RsaI GTAC 4 cut(s) 236, 1233, 1426, 1856
RsaNI GTAC 4 cut(s) 235, 1232, 1425, 1855
SalI GTCGAC 2 cut(s) 838, 1759
SapI GCTCTTC 1 cut(s) 1674
SaqAI TTAA 3 cut(s) 849, 1910, 2058
Sau3AI GATC 7 cut(s) 258, 585, 671, 1127, 1621, 1767, 1831
Sau96I GGNCC 3 cut(s) 608, 1529, 1891
ScaI AGTACT 1 cut(s) 1426
SchI GAGTC 3 cut(s) 791, 845, 1766
SfaNI GCATC 3 cut(s) 945, 1306, 1348
SfcI CTRYAG 1 cut(s) 2031
SinI GGWCC 2 cut(s) 1529, 1891
SmlI CTYRAG 3 cut(s) 254, 709, 1980
SmoI CTYRAG 3 cut(s) 254, 709, 1980
SphI GCATGC 1 cut(s) 1697
Sse9I AATT 8 cut(s) 200, 290, 1185, 1483, 1587, 1625, 1709, 1798
SspMI CTAG 5 cut(s) 188, 684, 855, 1253, 1493
StyI CCWWGG 2 cut(s) 316, 1237
TaaI ACNGT 3 cut(s) 328, 805, 1293
TaiI ACGT 3 cut(s) 429, 522, 1067
TaqII GACCGA 1 cut(s) 1254
TasI AATT 8 cut(s) 200, 290, 1185, 1483, 1587, 1625, 1709, 1798
TatI WGTACW 2 cut(s) 1424, 1854
TauI GCSGC 5 cut(s) 197, 230, 371, 501, 779
TfiI GAWTC 6 cut(s) 1084, 1369, 1601, 1915, 1976, 2027
Tru1I TTAA 3 cut(s) 849, 1910, 2058
Tru9I TTAA 3 cut(s) 849, 1910, 2058
TseFI GTSAC 3 cut(s) 698, 1395, 1704
TseI GCWGC 8 cut(s) 365, 415, 997, 1097, 1697, 1876, 1996, 2033
Tsp45I GTSAC 3 cut(s) 698, 1395, 1704
TspDTI ATGAA 3 cut(s) 1702, 1840, 2003
TspGWI ACGGA 2 cut(s) 517, 1438
VpaK11BI GGWCC 2 cut(s) 1529, 1891
VspI ATTAAT 1 cut(s) 849
XapI RAATTY 2 cut(s) 1483, 1798
XbaI TCTAGA 2 cut(s) 683, 1492
XceI RCATGY 1 cut(s) 1697
XmiI GTMKAC 2 cut(s) 839, 1760
XspI CTAG 5 cut(s) 188, 684, 855, 1253, 1493
ZraI GACGTC 1 cut(s) 520
ZrmI AGTACT 1 cut(s) 1426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.