RchiOBHm_Chr2g0128231

Arogenate dehydrogenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003175
Physical Location & Seq
Reverse (-)
43168886 .. 43172542
3657 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50003

Sequence Viewer

Length: 1806 bp
ATGCTCAAGATTGCCATCATTGGTTTTGGCAACTTGGGCCAATCCCTTGCCAAGACCTTTGCTAACCTAGGCCACATCGTCCTTGCACATTCTAGATGTGACCACTCCAAAATGGCCCAAGACCTCGGCATTTCATATTTGTCTGACCCGCAAGACCTCTGTGAACAGAATCCACAGGTCATTTTGCTATGCACCTCCATAATCTCCACAGAATCCGTCCTCAAATCTTTACCTCTCCAACGCCTTAGGCATGACACATTGTTTGTTGACATGCTTCCTGTCAAAGAGTTTTCAAAGGCATTGTTACTCAAGTTGTTGCCTAGCTATTTTGATGTCCTTTGCACCAACCCAATGTTCGGAGTTGAGAGAGGCAAACATGAATGGAATGGCATGTTTTTTGTGTACGAAAATGCTCGAATTGGCTCTGACGGAGTGGGGATTTCTCTCTGTGATAACTTCTTAAACATATTTGAGAAAGAAGGGTGTAGGATGGTGGAAGTAAGCTGTTCAGAGCAGGATAAGTATGCCGCAGGTTCACAGTTTATTACCCACGCAGTTGGGAGAGTCTTGGGGATGTTGCCATTGGAATCGACTCCAATAAATACAAAAGGTTATGAAACATTGTTGCATTTGGTGGACAACACAGCTAGGGATAGTTTTGATTTATATTATGAGTTGTTCATGTACAACAAAAATGCATTTGAGATGTTTGAGAGGTTGGACTTGGCTTTTGACACTTTGAAGAAACAGCTTTTTGGTTATTTGCATGATGTTGTGAGAAAGCAAGTATTTGATAATGCAGAGAAGGTAAAAATGGGGCACGAGGAGTACAAGGTGTCTAACCCATCTCACAATGGAGCTGCCTCCGAATCTTCTTCAAAAGCTATGAGGTCGCATAAGATTGCTCAGGTATCTAACAGTAAAGCACAGATAATATCTGACCACTTTGATGACAACTCAAGGCTCAAGATTGCAGTTGTTGGTTTTGGGAACTTTGGTCAGTTTCTTGCTAAAACTATTATACGTCAAGGTCATATAGTTCTAGCCTATTCTCGATCAGACTACTATGATGTGGCTCAAAAGTTGGGGGTAACTTACTGCTCTGATGTCGAGGAACTTTGCAAAGAGAATCCAGAAGTGATACTTTTTTGTACCTCTATTCTTTCAACTGAGAAAGTTCTCAGGTCATTTCCAGTTCAGAGGTTGATGAGGAATACTTTGTTTGTTGATGTTCTTTCTGTTAAAGAATTTCCAAAAAACTTGTTTCTTCAAAAGTTGCCATTGAATTTTGATATTCTCTGTACTCATCCTATGTTTGGACCAGAGAGTGGTAAAAATGGGTGGGAGGCTCTTCCTTTTGTTTATGATAAGGTCAGGGTGGGAAGTGATGAATCAAGGGTATCACTCTGTGACCGATTTCTTGATATCTTTGCTCTTGAAGGGTGTCGAATGGTTGAAATGTCTTGTGCAGAGCATGATAGGCATGCAGCAGGGTCACAATTCCTTACACACACAATAGGAAGGGTTTTGGAGAAGCTAGGTTTGGAGTCGACACCTATCGTTACAAATGGTTACAAGACTTTATTGAATTTGGTGGAGACTACAGTAGGAGATAGTTTTGATCTTTACTATGGCTTGTTCATGTATAATGTAAATGCAATGGACCAGCTAAATAGATTGGGCATGGCTTTTGACTCATTAGAGGAGCAGTTTTTGGGGCGTTTGCATGGTGTTCTCCATAAGCAACATTCTGAGAATGCAAGCAAAAGCTTGTTGCCAAATCATCCAAGAATGCAGCTCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00400 ko01100 ko01110 ko01230 map00400 map01100 map01110 map01230
Pfam Domains
Protein Families
F420_oxidored PDH

Protein Analysis

601

Amino Acids

67.88

Weight (kDa)

6.13

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F420_oxidored PF03807 3 - 92 1.6e-09 NADP oxidoreductase coenzyme F420-dependent
2-Hacid_dh_C PF02826 3 - 90 8.6e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
TYRAAT1_C PF26213 189 - 262 4.2e-33 TYRAAT1-like, C-terminal domain
F420_oxidored PF03807 323 - 412 2.7e-08 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 337 - 445 2.7e-08 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 509 - 582 5.4e-31 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 521
AccB7I CCANNNNNTGG 1 cut(s) 1328
AccI GTMKAC 1 cut(s) 1550
AciI CCGC 2 cut(s) 149, 528
AcsI RAATTY 3 cut(s) 1247, 1285, 1588
AdeI CACNNNGTG 1 cut(s) 1409
AfaI GTAC 5 cut(s) 404, 686, 830, 1153, 1303
AfiI CCNNNNNNNGG 3 cut(s) 356, 1316, 1328
AgsI TTSAA 9 cut(s) 294, 742, 879, 1167, 1271, 1285, 1439, 1457, 1588
AjuI GAANNNNNNNTTGG 4 cut(s) 338, 370, 1526, 1558
Alw26I GTCTC 1 cut(s) 1592
AoxI GGCC 3 cut(s) 37, 70, 114
ApeKI GCWGC 3 cut(s) 860, 1487, 1795
ApoI RAATTY 3 cut(s) 1247, 1285, 1588
ArsI GACNNNNNNTTYG 4 cut(s) 245, 277, 713, 745
AspA2I CCTAGG 1 cut(s) 67
AspS9I GGNCC 4 cut(s) 37, 115, 1319, 1663
AvaII GGWCC 2 cut(s) 1319, 1663
AvrII CCTAGG 1 cut(s) 67
AxyI CCTNAGG 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 822
BauI CACGAG 1 cut(s) 821
BbvI GCAGC 2 cut(s) 847, 1499
BccI CCATC 3 cut(s) 23, 484, 853
BcoDI GTCTC 1 cut(s) 1592
BfaI CTAG 6 cut(s) 68, 93, 321, 648, 1043, 1538
BfmI CTRYAG 1 cut(s) 1602
BfuAI ACCTGC 1 cut(s) 521
BisI GCNGC 4 cut(s) 528, 861, 1488, 1796
BlnI CCTAGG 1 cut(s) 67
BlsI GCNGC 4 cut(s) 529, 862, 1489, 1797
Bme18I GGWCC 2 cut(s) 1319, 1663
BmgT120I GGNCC 4 cut(s) 37, 115, 1319, 1663
Bpu10I CCTNAGC 1 cut(s) 906
BpuEI CTTGAG 3 cut(s) 293, 943, 950
BsaBI GATNNNNATC 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 67, 124
Bsc4I CCNNNNNNNGG 3 cut(s) 356, 1316, 1328
Bse1I ACTGG 1 cut(s) 1193
Bse21I CCTNAGG 1 cut(s) 245
Bse3DI GCAATG 1 cut(s) 1665
Bse8I GATNNNNATC 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 67, 124
BseGI GGATG 4 cut(s) 495, 579, 1306, 1783
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 3 cut(s) 356, 1316, 1328
BseMI GCAATG 1 cut(s) 1665
BseMII CTCAG 4 cut(s) 920, 1161, 1195, 1743
BseNI ACTGG 1 cut(s) 1193
BseRI GAGGAG 2 cut(s) 839, 1718
BseSI GKGCMC 1 cut(s) 822
BseXI GCAGC 2 cut(s) 847, 1499
BsgI GTGCAG 1 cut(s) 1488
BshFI GGCC 3 cut(s) 39, 72, 116
BslI CCNNNNNNNGG 3 cut(s) 356, 1316, 1328
BsmAI GTCTC 1 cut(s) 1592
BsmI GAATGC 2 cut(s) 1762, 1797
BsnI GGCC 3 cut(s) 39, 72, 116
Bsp1286I GDGCHC 1 cut(s) 822
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 2 cut(s) 1055, 1621
BspACI CCGC 2 cut(s) 149, 528
BspANI GGCC 3 cut(s) 39, 72, 116
BspCNI CTCAG 4 cut(s) 919, 1162, 1194, 1744
BspMI ACCTGC 1 cut(s) 521
BspQI GCTCTTC 1 cut(s) 1356
BsrDI GCAATG 1 cut(s) 1665
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 1 cut(s) 1193
BssECI CCNNGG 2 cut(s) 67, 124
BssMI GATC 2 cut(s) 1055, 1621
BssSI CACGAG 1 cut(s) 821
BssT1I CCWWGG 1 cut(s) 67
Bst2BI CACGAG 1 cut(s) 821
Bst4CI ACNGT 3 cut(s) 540, 920, 1606
Bst6I CTCTTC 1 cut(s) 1356
BstAUI TGTACA 1 cut(s) 684
BstC8I GCNNGC 2 cut(s) 1485, 1762
BstDEI CTNAG 5 cut(s) 245, 906, 1170, 1181, 1752
BstF5I GGATG 4 cut(s) 495, 579, 1306, 1783
BstKTI GATC 2 cut(s) 1058, 1624
BstMAI GTCTC 1 cut(s) 1592
BstMBI GATC 2 cut(s) 1055, 1621
BstMWI GCNNNNNNNGC 2 cut(s) 36, 1480
BstNSI RCATGY 3 cut(s) 274, 394, 1487
BstSFI CTRYAG 1 cut(s) 1602
BstSLI GKGCMC 1 cut(s) 822
BstV1I GCAGC 2 cut(s) 847, 1499
BstXI CCANNNNNNTGG 1 cut(s) 557
Bsu36I CCTNAGG 1 cut(s) 245
BsuRI GGCC 3 cut(s) 39, 72, 116
BtsCI GGATG 4 cut(s) 495, 579, 1306, 1783
BveI ACCTGC 1 cut(s) 521
Cac8I GCNNGC 2 cut(s) 1485, 1762
Cfr13I GGNCC 4 cut(s) 37, 115, 1319, 1663
Csp6I GTAC 5 cut(s) 403, 685, 829, 1152, 1302
CviQI GTAC 5 cut(s) 403, 685, 829, 1152, 1302
DdeI CTNAG 5 cut(s) 245, 906, 1170, 1181, 1752
DpnI GATC 2 cut(s) 1057, 1623
DpnII GATC 2 cut(s) 1055, 1621
DraIII CACNNNGTG 1 cut(s) 1409
Eam1104I CTCTTC 1 cut(s) 1356
EarI CTCTTC 1 cut(s) 1356
Eco130I CCWWGG 1 cut(s) 67
Eco32I GATATC 1 cut(s) 1426
Eco47I GGWCC 2 cut(s) 1319, 1663
Eco81I CCTNAGG 1 cut(s) 245
EcoRV GATATC 1 cut(s) 1426
EcoT14I CCWWGG 1 cut(s) 67
EcoT22I ATGCAT 1 cut(s) 700
ErhI CCWWGG 1 cut(s) 67
FalI AAGNNNNNCTT 2 cut(s) 1128, 1160
FauI CCCGC 1 cut(s) 156
FblI GTMKAC 1 cut(s) 1550
Fnu4HI GCNGC 4 cut(s) 528, 861, 1488, 1796
FokI GGATG 4 cut(s) 502, 586, 1293, 1770
Fsp4HI GCNGC 4 cut(s) 528, 861, 1488, 1796
FspBI CTAG 6 cut(s) 68, 93, 321, 648, 1043, 1538
GluI GCNGC 4 cut(s) 528, 861, 1488, 1796
HaeIII GGCC 3 cut(s) 39, 72, 116
HincII GTYRAC 2 cut(s) 268, 1551
HindII GTYRAC 2 cut(s) 268, 1551
HindIII AAGCTT 1 cut(s) 1768
Hpy166II GTNNAC 6 cut(s) 164, 268, 403, 536, 637, 1551
Hpy188III TCNNGA 7 cut(s) 7, 93, 967, 1053, 1133, 1421, 1436
Hpy8I GTNNAC 6 cut(s) 164, 268, 403, 536, 637, 1551
HpyAV CCTTC 4 cut(s) 473, 799, 1433, 1515
HpyCH4III ACNGT 3 cut(s) 540, 920, 1606
HpyCH4IV ACGT 1 cut(s) 1024
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 1480
HpyF3I CTNAG 5 cut(s) 245, 906, 1170, 1181, 1752
HpySE526I ACGT 1 cut(s) 1024
Kzo9I GATC 2 cut(s) 1055, 1621
LguI GCTCTTC 1 cut(s) 1356
LmnI GCTCC 3 cut(s) 857, 1705, 1803
Lsp1109I GCAGC 2 cut(s) 847, 1499
MaeI CTAG 6 cut(s) 68, 93, 321, 648, 1043, 1538
MaeII ACGT 1 cut(s) 1024
MaeIII GTNAC 7 cut(s) 98, 303, 1090, 1409, 1494, 1561, 1571
MalI GATC 2 cut(s) 1057, 1623
MboI GATC 2 cut(s) 1055, 1621
MboII GAAGA 5 cut(s) 754, 864, 867, 1259, 1343
MhlI GDGCHC 1 cut(s) 822
MluCI AATT 5 cut(s) 417, 1247, 1285, 1499, 1588
MlyI GAGTC 4 cut(s) 573, 586, 1556, 1688
MmeI TCCRAC 2 cut(s) 262, 699
Mph1103I ATGCAT 1 cut(s) 700
MseI TTAA 2 cut(s) 461, 1242
MslI CAYNNNNRTG 1 cut(s) 948
Mva1269I GAATGC 2 cut(s) 1762, 1797
MwoI GCNNNNNNNGC 2 cut(s) 36, 1480
NdeII GATC 2 cut(s) 1055, 1621
NmeAIII GCCGAG 1 cut(s) 105
NmuCI GTSAC 3 cut(s) 98, 1409, 1494
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 3 cut(s) 274, 394, 1487
PaeI GCATGC 1 cut(s) 1487
PciSI GCTCTTC 1 cut(s) 1356
PctI GAATGC 2 cut(s) 1762, 1797
PfeI GAWTC 6 cut(s) 169, 212, 587, 869, 1129, 1391
PflMI CCANNNNNTGG 1 cut(s) 1328
PkrI GCNGC 4 cut(s) 529, 862, 1489, 1797
PleI GAGTC 4 cut(s) 572, 586, 1555, 1688
PpsI GAGTC 4 cut(s) 572, 586, 1555, 1688
PspPI GGNCC 4 cut(s) 37, 115, 1319, 1663
RsaI GTAC 5 cut(s) 404, 686, 830, 1153, 1303
RsaNI GTAC 5 cut(s) 403, 685, 829, 1152, 1302
RseI CAYNNNNRTG 1 cut(s) 948
SalI GTCGAC 1 cut(s) 1549
SapI GCTCTTC 1 cut(s) 1356
SaqAI TTAA 2 cut(s) 461, 1242
SatI GCNGC 4 cut(s) 528, 861, 1488, 1796
Sau3AI GATC 2 cut(s) 1055, 1621
Sau96I GGNCC 4 cut(s) 37, 115, 1319, 1663
SchI GAGTC 4 cut(s) 573, 586, 1556, 1688
SduI GDGCHC 1 cut(s) 822
SfcI CTRYAG 1 cut(s) 1602
SinI GGWCC 2 cut(s) 1319, 1663
SmiMI CAYNNNNRTG 1 cut(s) 948
SmlI CTYRAG 4 cut(s) 5, 308, 958, 965
SmoI CTYRAG 4 cut(s) 5, 308, 958, 965
SphI GCATGC 1 cut(s) 1487
Sse9I AATT 5 cut(s) 417, 1247, 1285, 1499, 1588
SsiI CCGC 2 cut(s) 149, 528
SspMI CTAG 6 cut(s) 68, 93, 321, 648, 1043, 1538
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 3 cut(s) 540, 920, 1606
TaiI ACGT 1 cut(s) 1027
TaqI TCGA 6 cut(s) 415, 590, 1054, 1110, 1447, 1550
TaqII GACCGA 1 cut(s) 1428
TasI AATT 5 cut(s) 417, 1247, 1285, 1499, 1588
TatI WGTACW 3 cut(s) 684, 828, 1301
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 6 cut(s) 169, 212, 587, 869, 1129, 1391
Tru1I TTAA 2 cut(s) 461, 1242
Tru9I TTAA 2 cut(s) 461, 1242
TseFI GTSAC 3 cut(s) 98, 1409, 1494
TseI GCWGC 3 cut(s) 860, 1487, 1795
Tsp45I GTSAC 3 cut(s) 98, 1409, 1494
TspDTI ATGAA 6 cut(s) 123, 393, 630, 670, 1404, 1630
TspGWI ACGGA 2 cut(s) 205, 444
Van91I CCANNNNNTGG 1 cut(s) 1328
VpaK11BI GGWCC 2 cut(s) 1319, 1663
XapI RAATTY 3 cut(s) 1247, 1285, 1588
XbaI TCTAGA 1 cut(s) 92
XceI RCATGY 3 cut(s) 274, 394, 1487
XmaJI CCTAGG 1 cut(s) 67
XmiI GTMKAC 1 cut(s) 1550
XspI CTAG 6 cut(s) 68, 93, 321, 648, 1043, 1538
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.