Rroxscaffold_2G00115470

Arogenate dehydrogenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003175
Physical Location & Seq
Forward (+)
41744523 .. 41747769
3247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00115470.1

Sequence Viewer

Length: 1821 bp
ATGCTGAAGATTGCCATCATCGGCTTTGGCAAATTGGGACAATCCCTTGCCAAGACCTTTGCTGACCTAGGCCACATGGTCCTTGCACATTCTATATGTGACCACTCCAAAATGGCCCAAGACCTCGGCATTTCATATTTCTCTGACCCGCAAGACCTCTGTGAACAGAATCCACAGGTCATTTTGCTATGCACCTCCATAATCTCCACAGAATCCGTCCTCAGATCTTTGCCTCTCCTGAGCCTTAAGCATGACACATTGTTTGTTGACATGCTTCCTGTCAAAGAGTTTTCAAAAGCACTGTTACTCAAGTTGTTGCCTAGCTATTTAGATGTTCTTTGCACCAACCCAATGTTCGGAGTTGAGAGAGGCAAACATGAGTGGAATGGCATGTTTTTTGTGTACGAAAAGGTTCGAATTGGCTCTGACGGAGCGGGGATTTCTTTCTGTGATAACTTCTTAAACATGTTTGAGAAAGAAGGGTGTAGAATGGTGGAAGTAAGCTGTTCTGAGCAGGATAAGTATGTCGCAGGTTCGCAGTTTATTACCCACACAGTTGGGAGAGTCTTGGGGATGTTGACATTGGAATCGACTCCAATAAATACAAAAGGTTATGAGACATTGTTGGATTTAATGGACAACACAGCTAGGGATGGTTTTGATTTATATTATGAGTTTTTCATGTACAACAAAAATGCATTTGAGATGTTTGAGAGGTTGGACTTGGCTTTTCACACTTTGAAGAAACAGCTTTTTGGTTATTTGTATGATGTTGTGAGAAAGCAAGTATTTGATAATGCAGAGAAGGTAAAAATGGGGCACGAGGAGTATAAGGTGTCTAACACATCCCACAATACAGCTGCCTCGGAATCTTCTTCAAAAGCTATGAGGTTTATTGCAACCTTCAGGTCGCATAAGATTGCTCAGGTATCTAACTGTAAAGCGCTGATATCTGACCACTTTGGTGACAACTCAAGGCTCAAGATTGCAATTGTTGGTTTTGGGAACTTTGGTCAGTTTCTTGCTAAAACTATTATACGTCAAGGTCATATAGTTCTAGCCTATTCTCGATCAGACTACTATGATGTGGCTCAAAAGTTGGGGGTAATTTACTTCTCTGATGTCGAGGAACTTTGCAAAGAGCATCCAGAAGTGATACTTTTTTGTACCTCTATTCTTTCAACTGAGAAAGTTCTCAGGTCATTTCCAGTTCAGAGGTTGATGAGGAATACTTTGTTTGTTGATGTTCTTTCAGTTAAAGAATTTCCAAAAAACTTGTTTCTTCAAAAGTTGCCATTGAATTTTGATATTCTCTGTACGCATCCTATGTTTGGACCAGAGAGTGGTAAAAATGGGTGGGAGGCTCTTCCTTTTGTTTATGATAAGGTCAGGGTGGGAAGTGACGAATCAAGGGTATCACTCTGTGACCGATTTCTTGATATCTTTGCTCTTGAAGGGTGTCAAATGGTTGAAATGTCTTGTGCAGAGCATGATAGGCATGCAGCAGGGTCACAATTCCTTACGCACACAATAGGAAGGGTTTTGGAAAAGCTGGGTTTGGAGTCGACACCTATCTTTACAAATGGTTACAAGACTTTATTGAATTTGGTGGAGAATACAGTAGGAGATAGTTTTGATCTTTACTATGGCTTGTTCATGTATAATGTAAATGCAATGGACCAGCTAAATAGATTGGGCATGGCTTTTGACTCATTAGAGGAGCAGTTTCTGAGGCGTTTGCATGGTGTTCTCCATAAGCAACATTCTGAGAATGCAAGCAAAACCTTGTTGCCAACTCATCCAAGAATGCAGCTCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00400 ko01100 ko01110 ko01230 map00400 map01100 map01110 map01230
Pfam Domains
Protein Families
F420_oxidored PDH

Protein Analysis

606

Amino Acids

68.82

Weight (kDa)

6.2

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F420_oxidored PF03807 3 - 92 7.3e-08 NADP oxidoreductase coenzyme F420-dependent
2-Hacid_dh_C PF02826 3 - 90 8e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
TYRAAT1_C PF26213 189 - 262 2.3e-29 TYRAAT1-like, C-terminal domain
F420_oxidored PF03807 328 - 417 6.4e-08 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 342 - 450 1e-07 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 514 - 587 4.2e-31 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 521
AccB7I CCANNNNNTGG 1 cut(s) 1343
AccBSI CCGCTC 1 cut(s) 434
AccI GTMKAC 1 cut(s) 1565
AciI CCGC 2 cut(s) 149, 434
AcsI RAATTY 3 cut(s) 1262, 1300, 1603
AcuI CTGAAG 2 cut(s) 26, 889
AdeI CACNNNGTG 1 cut(s) 1424
AfaI GTAC 4 cut(s) 404, 686, 1168, 1318
AfeI AGCGCT 1 cut(s) 945
AfiI CCNNNNNNNGG 3 cut(s) 356, 1331, 1343
AflII CTTAAG 1 cut(s) 245
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 9 cut(s) 294, 742, 879, 1182, 1286, 1300, 1454, 1472, 1603
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AleI CACNNNNGTG 1 cut(s) 963
AluBI AGCT 9 cut(s) 324, 504, 647, 751, 860, 884, 1552, 1684, 1813
AluI AGCT 9 cut(s) 324, 504, 647, 751, 860, 884, 1552, 1684, 1813
Alw26I GTCTC 1 cut(s) 611
AlwNI CAGNNNCTG 1 cut(s) 1729
Aor51HI AGCGCT 1 cut(s) 945
AoxI GGCC 2 cut(s) 70, 114
ApeKI GCWGC 3 cut(s) 860, 1502, 1810
ApoI RAATTY 3 cut(s) 1262, 1300, 1603
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
Asp700I GAANNNNTTC 1 cut(s) 411
AspA2I CCTAGG 1 cut(s) 67
AspLEI GCGC 1 cut(s) 946
AspS9I GGNCC 4 cut(s) 79, 115, 1334, 1678
AsuHPI GGTGA 1 cut(s) 977
AsuII TTCGAA 1 cut(s) 415
AvaII GGWCC 3 cut(s) 79, 1334, 1678
AvrII CCTAGG 1 cut(s) 67
BaeGI GKGCMC 1 cut(s) 822
BauI CACGAG 1 cut(s) 821
BbvI GCAGC 2 cut(s) 847, 1514
BccI CCATC 2 cut(s) 23, 647
BcoDI GTCTC 1 cut(s) 611
BfaI CTAG 4 cut(s) 68, 321, 648, 1058
BfoI RGCGCY 1 cut(s) 947
BfrI CTTAAG 1 cut(s) 245
BfuAI ACCTGC 1 cut(s) 521
BglII AGATCT 1 cut(s) 224
BisI GCNGC 3 cut(s) 861, 1503, 1811
BlnI CCTAGG 1 cut(s) 67
BlsI GCNGC 3 cut(s) 862, 1504, 1812
Bme18I GGWCC 3 cut(s) 79, 1334, 1678
BmgT120I GGNCC 4 cut(s) 79, 115, 1334, 1678
BmsI GCATC 2 cut(s) 1153, 1330
Bpu10I CCTNAGC 2 cut(s) 239, 924
Bpu14I TTCGAA 1 cut(s) 415
BpuEI CTTGAG 3 cut(s) 293, 958, 965
BsaBI GATNNNNATC 1 cut(s) 14
BsaJI CCNNGG 3 cut(s) 67, 124, 864
Bsc4I CCNNNNNNNGG 3 cut(s) 356, 1331, 1343
Bse1I ACTGG 1 cut(s) 1208
Bse3DI GCAATG 1 cut(s) 1680
Bse8I GATNNNNATC 1 cut(s) 14
BseDI CCNNGG 3 cut(s) 67, 124, 864
BseGI GGATG 6 cut(s) 579, 658, 845, 1144, 1321, 1798
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 3 cut(s) 356, 1331, 1343
BseMI GCAATG 1 cut(s) 1680
BseMII CTCAG 8 cut(s) 230, 235, 501, 938, 1176, 1210, 1721, 1758
BseNI ACTGG 1 cut(s) 1208
BseRI GAGGAG 2 cut(s) 839, 1733
BseSI GKGCMC 1 cut(s) 822
BseXI GCAGC 2 cut(s) 847, 1514
BseYI CCCAGC 1 cut(s) 1552
BsgI GTGCAG 1 cut(s) 1503
BshFI GGCC 2 cut(s) 72, 116
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 3 cut(s) 356, 1331, 1343
BsmAI GTCTC 1 cut(s) 611
BsmFI GGGAC 1 cut(s) 51
BsmI GAATGC 2 cut(s) 1777, 1812
BsnI GGCC 2 cut(s) 72, 116
Bsp119I TTCGAA 1 cut(s) 415
Bsp1286I GDGCHC 1 cut(s) 822
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 3 cut(s) 224, 1070, 1636
BspACI CCGC 2 cut(s) 149, 434
BspANI GGCC 2 cut(s) 72, 116
BspCNI CTCAG 8 cut(s) 231, 234, 502, 937, 1177, 1209, 1722, 1759
BspMI ACCTGC 1 cut(s) 521
BspQI GCTCTTC 1 cut(s) 1371
BspT104I TTCGAA 1 cut(s) 415
BspTI CTTAAG 1 cut(s) 245
BsrBI CCGCTC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 1680
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 1 cut(s) 1208
BssECI CCNNGG 3 cut(s) 67, 124, 864
BssMI GATC 3 cut(s) 224, 1070, 1636
BssSI CACGAG 1 cut(s) 821
BssT1I CCWWGG 1 cut(s) 67
Bst2BI CACGAG 1 cut(s) 821
Bst4CI ACNGT 4 cut(s) 303, 556, 938, 1621
Bst6I CTCTTC 1 cut(s) 1371
BstAFI CTTAAG 1 cut(s) 245
BstAUI TGTACA 1 cut(s) 684
BstBI TTCGAA 1 cut(s) 415
BstC8I GCNNGC 2 cut(s) 1500, 1777
BstDEI CTNAG 8 cut(s) 221, 239, 510, 924, 1185, 1196, 1730, 1767
BstF5I GGATG 6 cut(s) 579, 658, 845, 1144, 1321, 1798
BstH2I RGCGCY 1 cut(s) 947
BstHHI GCGC 1 cut(s) 946
BstKTI GATC 3 cut(s) 227, 1073, 1639
BstMAI GTCTC 1 cut(s) 611
BstMBI GATC 3 cut(s) 224, 1070, 1636
BstMWI GCNNNNNNNGC 1 cut(s) 1495
BstNSI RCATGY 4 cut(s) 274, 394, 469, 1502
BstSLI GKGCMC 1 cut(s) 822
BstV1I GCAGC 2 cut(s) 847, 1514
BstX2I RGATCY 1 cut(s) 224
BstXI CCANNNNNNTGG 1 cut(s) 557
BstYI RGATCY 1 cut(s) 224
BsuRI GGCC 2 cut(s) 72, 116
BtsCI GGATG 6 cut(s) 579, 658, 845, 1144, 1321, 1798
BtsIMutI CAGTG 1 cut(s) 299
BveI ACCTGC 1 cut(s) 521
Cac8I GCNNGC 2 cut(s) 1500, 1777
CaiI CAGNNNCTG 1 cut(s) 1729
CfoI GCGC 1 cut(s) 946
Cfr13I GGNCC 4 cut(s) 79, 115, 1334, 1678
Csp6I GTAC 4 cut(s) 403, 685, 1167, 1317
CviQI GTAC 4 cut(s) 403, 685, 1167, 1317
DdeI CTNAG 8 cut(s) 221, 239, 510, 924, 1185, 1196, 1730, 1767
DpnI GATC 3 cut(s) 226, 1072, 1638
DpnII GATC 3 cut(s) 224, 1070, 1636
DraIII CACNNNGTG 1 cut(s) 1424
Eam1104I CTCTTC 1 cut(s) 1371
EarI CTCTTC 1 cut(s) 1371
Eco130I CCWWGG 1 cut(s) 67
Eco32I GATATC 2 cut(s) 951, 1441
Eco47I GGWCC 3 cut(s) 79, 1334, 1678
Eco47III AGCGCT 1 cut(s) 945
Eco57I CTGAAG 2 cut(s) 26, 889
EcoRV GATATC 2 cut(s) 951, 1441
EcoT14I CCWWGG 1 cut(s) 67
EcoT22I ATGCAT 1 cut(s) 700
ErhI CCWWGG 1 cut(s) 67
FalI AAGNNNNNCTT 2 cut(s) 1143, 1175
FaqI GGGAC 1 cut(s) 51
FauI CCCGC 2 cut(s) 156, 427
FblI GTMKAC 1 cut(s) 1565
Fnu4HI GCNGC 3 cut(s) 861, 1503, 1811
FokI GGATG 6 cut(s) 586, 665, 832, 1131, 1308, 1785
Fsp4HI GCNGC 3 cut(s) 861, 1503, 1811
FspBI CTAG 4 cut(s) 68, 321, 648, 1058
GlaI GCGC 1 cut(s) 945
GluI GCNGC 3 cut(s) 861, 1503, 1811
GsaI CCCAGC 1 cut(s) 1556
HaeII RGCGCY 1 cut(s) 947
HaeIII GGCC 2 cut(s) 72, 116
HhaI GCGC 1 cut(s) 946
Hin6I GCGC 1 cut(s) 944
HinP1I GCGC 1 cut(s) 944
HincII GTYRAC 3 cut(s) 268, 579, 1566
HindII GTYRAC 3 cut(s) 268, 579, 1566
HinfI GANTC 9 cut(s) 169, 212, 564, 587, 592, 869, 1406, 1562, 1709
HphI GGTGA 1 cut(s) 977
Hpy166II GTNNAC 5 cut(s) 164, 268, 403, 579, 1566
Hpy188III TCNNGA 6 cut(s) 238, 982, 1068, 1148, 1436, 1451
Hpy8I GTNNAC 5 cut(s) 164, 268, 403, 579, 1566
HpyAV CCTTC 5 cut(s) 473, 799, 913, 1448, 1530
HpyCH4III ACNGT 4 cut(s) 303, 556, 938, 1621
HpyCH4IV ACGT 1 cut(s) 1039
HpyF10VI GCNNNNNNNGC 1 cut(s) 1495
HpyF3I CTNAG 8 cut(s) 221, 239, 510, 924, 1185, 1196, 1730, 1767
HpySE526I ACGT 1 cut(s) 1039
HspAI GCGC 1 cut(s) 944
Kzo9I GATC 3 cut(s) 224, 1070, 1636
LguI GCTCTTC 1 cut(s) 1371
LmnI GCTCC 3 cut(s) 431, 1720, 1818
Lsp1109I GCAGC 2 cut(s) 847, 1514
LweI GCATC 2 cut(s) 1153, 1330
MaeI CTAG 4 cut(s) 68, 321, 648, 1058
MaeII ACGT 1 cut(s) 1039
MaeIII GTNAC 7 cut(s) 98, 303, 965, 1400, 1424, 1509, 1586
MalI GATC 3 cut(s) 226, 1072, 1638
MbiI CCGCTC 1 cut(s) 434
MboI GATC 3 cut(s) 224, 1070, 1636
MboII GAAGA 6 cut(s) 19, 754, 864, 867, 1274, 1358
MfeI CAATTG 1 cut(s) 990
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 1 cut(s) 822
MluCI AATT 8 cut(s) 32, 417, 990, 1107, 1262, 1300, 1514, 1603
MlyI GAGTC 4 cut(s) 573, 586, 1571, 1703
MmeI TCCRAC 2 cut(s) 606, 699
Mph1103I ATGCAT 1 cut(s) 700
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 4 cut(s) 246, 461, 632, 1257
MslI CAYNNNNRTG 1 cut(s) 963
MspA1I CMGCKG 1 cut(s) 860
MspCI CTTAAG 1 cut(s) 245
MunI CAATTG 1 cut(s) 990
Mva1269I GAATGC 2 cut(s) 1777, 1812
MwoI GCNNNNNNNGC 1 cut(s) 1495
NdeII GATC 3 cut(s) 224, 1070, 1636
NmeAIII GCCGAG 1 cut(s) 105
NmuCI GTSAC 5 cut(s) 98, 965, 1400, 1424, 1509
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 4 cut(s) 274, 394, 469, 1502
NspV TTCGAA 1 cut(s) 415
OliI CACNNNNGTG 1 cut(s) 963
PaeI GCATGC 1 cut(s) 1502
PciI ACATGT 1 cut(s) 465
PciSI GCTCTTC 1 cut(s) 1371
PctI GAATGC 2 cut(s) 1777, 1812
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 5 cut(s) 169, 212, 587, 869, 1406
PflMI CCANNNNNTGG 1 cut(s) 1343
PkrI GCNGC 3 cut(s) 862, 1504, 1812
PleI GAGTC 4 cut(s) 572, 586, 1570, 1703
PpsI GAGTC 4 cut(s) 572, 586, 1570, 1703
PscI ACATGT 1 cut(s) 465
PspFI CCCAGC 1 cut(s) 1552
PspPI GGNCC 4 cut(s) 79, 115, 1334, 1678
PstNI CAGNNNCTG 1 cut(s) 1729
PsuI RGATCY 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 860
RsaI GTAC 4 cut(s) 404, 686, 1168, 1318
RsaNI GTAC 4 cut(s) 403, 685, 1167, 1317
RseI CAYNNNNRTG 1 cut(s) 963
SalI GTCGAC 1 cut(s) 1564
SapI GCTCTTC 1 cut(s) 1371
SaqAI TTAA 4 cut(s) 246, 461, 632, 1257
SatI GCNGC 3 cut(s) 861, 1503, 1811
Sau3AI GATC 3 cut(s) 224, 1070, 1636
Sau96I GGNCC 4 cut(s) 79, 115, 1334, 1678
SchI GAGTC 4 cut(s) 573, 586, 1571, 1703
SduI GDGCHC 1 cut(s) 822
SfaNI GCATC 2 cut(s) 1153, 1330
SfuI TTCGAA 1 cut(s) 415
SinI GGWCC 3 cut(s) 79, 1334, 1678
SmiMI CAYNNNNRTG 1 cut(s) 963
SmlI CTYRAG 4 cut(s) 245, 308, 973, 980
SmoI CTYRAG 4 cut(s) 245, 308, 973, 980
SphI GCATGC 1 cut(s) 1502
Sse9I AATT 8 cut(s) 32, 417, 990, 1107, 1262, 1300, 1514, 1603
SsiI CCGC 2 cut(s) 149, 434
SspMI CTAG 4 cut(s) 68, 321, 648, 1058
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 4 cut(s) 303, 556, 938, 1621
TaiI ACGT 1 cut(s) 1042
TaqI TCGA 5 cut(s) 415, 590, 1069, 1125, 1565
TaqII GACCGA 1 cut(s) 1443
TasI AATT 8 cut(s) 32, 417, 990, 1107, 1262, 1300, 1514, 1603
TatI WGTACW 1 cut(s) 684
TfiI GAWTC 5 cut(s) 169, 212, 587, 869, 1406
Tru1I TTAA 4 cut(s) 246, 461, 632, 1257
Tru9I TTAA 4 cut(s) 246, 461, 632, 1257
TscAI CASTG 1 cut(s) 306
TseFI GTSAC 5 cut(s) 98, 965, 1400, 1424, 1509
TseI GCWGC 3 cut(s) 860, 1502, 1810
Tsp45I GTSAC 5 cut(s) 98, 965, 1400, 1424, 1509
TspDTI ATGAA 3 cut(s) 123, 670, 1645
TspGWI ACGGA 2 cut(s) 205, 444
TspRI CASTG 1 cut(s) 306
Van91I CCANNNNNTGG 1 cut(s) 1343
Vha464I CTTAAG 1 cut(s) 245
VpaK11BI GGWCC 3 cut(s) 79, 1334, 1678
XapI RAATTY 3 cut(s) 1262, 1300, 1603
XceI RCATGY 4 cut(s) 274, 394, 469, 1502
XmaJI CCTAGG 1 cut(s) 67
XmiI GTMKAC 1 cut(s) 1565
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 4 cut(s) 68, 321, 648, 1058
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.