Rroxscaffold_2G00115930

Arogenate dehydrogenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003175
Physical Location & Seq
Forward (+)
42548381 .. 42552161
3781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00115930.1

Sequence Viewer

Length: 1803 bp
ATGCTGAAGATTGCCATCATCGGCTTTGGCAACTTGGGACAATCCCTTGCCAAGACCTTTGCTGACCTAGGCCACATGGTCCTTGCACATTCTATATGTGACCACTCCAAAATGGCCCAAGACCTCGGCATTTCATATTTCTCTGACCCGCAAGACCTCTGTGAACAGAATCCACAGGTCATTTTGCTATGCACCTCCATAATCTCCACAGAATCCGTCCTCAGATCTTTGCCTCTCCTGAGCCTTAAGCATGACACATTGTTTGTTGACATGCTTCCTGTCAAAGAGTTTTCAAAAGCACTGTTACTCAAGTTGTTGCCTAGCTATTTAGATGTTCTTTGCACCAACCCAATGTTCGGAGTTGAGAGAGGCAAACATGAGTGGAATGGCATGTTTTTTGTGTACGAAAAGGTTCGAATTGGCTCTGACGGAGCGGGGATTTCTTTCTGTGATAACTTCTTAAACATGTTTGAGAAAGAAGGGTGTAGAATGGTGGAAGTAAGCTGTTCTGAGCAGGATAAGTATGTCGCAGGTTCGCAGTTTATTACCCACACAGTTGGGAGAGTCTTGGGGATGTTGACATTGGAATCGACTCCAATAAATACAAAAGGTTATGAGACATTGTTGGATTTAATGGACAACACAGCTAGGGATGGTTTTGATTTATATTATGAGTTTTTCATGTACAACAAAAATGCACTTGAGATGTTTGAGAGGTTGGACTTGGCTTTTCACACTTTGAAGAAACAGCTTTTTGGTTATTTGTATGATGTTGTGAGAAAGCAAGTATTTGATAATGCAGAGAAGGTAAAAATGGGGCACGAGGAGTATAAGGTGTCTAACACATCCCACAATACAGCTGCCTCGGAATCTTCTTCAAAAGCTATGAGGTCGCATAAGATTGCTCAGGTATCTAACTGTAAAGCGCTGATATCTGACCACTTTGGTGACAACTCAAGGCTCAAGATTGCAATTGTTGGTTTTGGGAACTTTGGTCAGTTTCTTGCTAAAACTATTATACGTCAAGGTCATATAGTTCTAGCCTATTCTCGATCAGACTACTATAATGTGGCTCAAAAGTTGGGGGTAATTTACTTCTCTGATGTCGAGGAACTTTGCAAAGAGCATCCAGAAGTGATACTTTTTTGTACCTCTATTCTTTCAACTGAGAAAGTTCTCAGGTCATTTCCAGTTCAGAGGTTGATGAGGAATACTTTGTTTGTTGATGTTCTTTCAGTTAAAGAATTTCCAAAAAACTTGTTTCTTCAAAAGTTGCCATTGAATTTTGATATTCTCTGTACGCATCCTATGTTTGGACCAGAGAGTGGTAAAAATGGGTGGGAGGCTCTTCCTTTTGTTTATGATAAGGTCAGGGTGGGAAGTGACGAATCAAGGGTATCACTCTGTGACCGATTTCTTGATATCTTTGCTCTTGAAGGGTGTCAAATGGTTGAAATGTCTTGTGCAGAGCATGATAGGCATGCAGCAGGGTCACAATTCCTTACGCACACAATAGGAAGGGTTTTGGAAAAGCTGGGTTTGGAGTCGACACCTATCTTTACAAATGGTTACAAGACTTTATTGAATTTGGTGGAGAATACAGTAGGAGATAGTTTTGATCTTTACTATGGCTTGTTCATGTATAATGTAAATGCAATGGACCAGCTAAATAGATTGGGCATGGCTTTTGACTCATTAGAGGAGCAGTTTCTGAGGCGTTTGCATGGTGTTCTCCATAAGCAACATTCTGAGAATGCAAGCAAAACCTTGTTGCCAACTCATCCAAGAATGCAGCTCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00400 ko01100 ko01110 ko01230 map00400 map01100 map01110 map01230
Pfam Domains
Protein Families
F420_oxidored PDH

Protein Analysis

600

Amino Acids

68.03

Weight (kDa)

6.13

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F420_oxidored PF03807 3 - 92 3.3e-08 NADP oxidoreductase coenzyme F420-dependent
2-Hacid_dh_C PF02826 3 - 90 6e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
TYRAAT1_C PF26213 189 - 262 1.1e-29 TYRAAT1-like, C-terminal domain
F420_oxidored PF03807 322 - 411 6.4e-08 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 336 - 444 4.4e-08 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 508 - 581 4.1e-31 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 521
AccB7I CCANNNNNTGG 1 cut(s) 1325
AccBSI CCGCTC 1 cut(s) 434
AccI GTMKAC 1 cut(s) 1547
AciI CCGC 2 cut(s) 149, 434
AcsI RAATTY 3 cut(s) 1244, 1282, 1585
AcuI CTGAAG 1 cut(s) 26
AdeI CACNNNGTG 1 cut(s) 1406
AfaI GTAC 4 cut(s) 404, 686, 1150, 1300
AfeI AGCGCT 1 cut(s) 927
AfiI CCNNNNNNNGG 3 cut(s) 356, 1313, 1325
AflII CTTAAG 1 cut(s) 245
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 9 cut(s) 294, 742, 879, 1164, 1268, 1282, 1436, 1454, 1585
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AleI CACNNNNGTG 1 cut(s) 945
AluBI AGCT 9 cut(s) 324, 504, 647, 751, 860, 884, 1534, 1666, 1795
AluI AGCT 9 cut(s) 324, 504, 647, 751, 860, 884, 1534, 1666, 1795
Alw26I GTCTC 1 cut(s) 611
AlwNI CAGNNNCTG 1 cut(s) 1711
Aor51HI AGCGCT 1 cut(s) 927
AoxI GGCC 2 cut(s) 70, 114
ApeKI GCWGC 3 cut(s) 860, 1484, 1792
ApoI RAATTY 3 cut(s) 1244, 1282, 1585
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
Asp700I GAANNNNTTC 1 cut(s) 411
AspA2I CCTAGG 1 cut(s) 67
AspLEI GCGC 1 cut(s) 928
AspS9I GGNCC 4 cut(s) 79, 115, 1316, 1660
AsuHPI GGTGA 1 cut(s) 959
AsuII TTCGAA 1 cut(s) 415
AvaII GGWCC 3 cut(s) 79, 1316, 1660
AvrII CCTAGG 1 cut(s) 67
BaeGI GKGCMC 1 cut(s) 822
BauI CACGAG 1 cut(s) 821
BbvI GCAGC 2 cut(s) 847, 1496
BccI CCATC 2 cut(s) 23, 647
BcoDI GTCTC 1 cut(s) 611
BfaI CTAG 4 cut(s) 68, 321, 648, 1040
BfoI RGCGCY 1 cut(s) 929
BfrI CTTAAG 1 cut(s) 245
BfuAI ACCTGC 1 cut(s) 521
BglII AGATCT 1 cut(s) 224
BisI GCNGC 3 cut(s) 861, 1485, 1793
BlnI CCTAGG 1 cut(s) 67
BlsI GCNGC 3 cut(s) 862, 1486, 1794
Bme18I GGWCC 3 cut(s) 79, 1316, 1660
BmgT120I GGNCC 4 cut(s) 79, 115, 1316, 1660
BmsI GCATC 2 cut(s) 1135, 1312
Bpu10I CCTNAGC 2 cut(s) 239, 906
Bpu14I TTCGAA 1 cut(s) 415
BpuEI CTTGAG 4 cut(s) 293, 722, 940, 947
BsaBI GATNNNNATC 1 cut(s) 14
BsaJI CCNNGG 3 cut(s) 67, 124, 864
Bsc4I CCNNNNNNNGG 3 cut(s) 356, 1313, 1325
Bse1I ACTGG 1 cut(s) 1190
Bse3DI GCAATG 1 cut(s) 1662
Bse8I GATNNNNATC 1 cut(s) 14
BseDI CCNNGG 3 cut(s) 67, 124, 864
BseGI GGATG 6 cut(s) 579, 658, 845, 1126, 1303, 1780
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 3 cut(s) 356, 1313, 1325
BseMI GCAATG 1 cut(s) 1662
BseMII CTCAG 8 cut(s) 230, 235, 501, 920, 1158, 1192, 1703, 1740
BseNI ACTGG 1 cut(s) 1190
BseRI GAGGAG 2 cut(s) 839, 1715
BseSI GKGCMC 1 cut(s) 822
BseXI GCAGC 2 cut(s) 847, 1496
BseYI CCCAGC 1 cut(s) 1534
BsgI GTGCAG 1 cut(s) 1485
BshFI GGCC 2 cut(s) 72, 116
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 3 cut(s) 356, 1313, 1325
BsmAI GTCTC 1 cut(s) 611
BsmFI GGGAC 1 cut(s) 51
BsmI GAATGC 2 cut(s) 1759, 1794
BsnI GGCC 2 cut(s) 72, 116
Bsp119I TTCGAA 1 cut(s) 415
Bsp1286I GDGCHC 1 cut(s) 822
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 3 cut(s) 224, 1052, 1618
BspACI CCGC 2 cut(s) 149, 434
BspANI GGCC 2 cut(s) 72, 116
BspCNI CTCAG 8 cut(s) 231, 234, 502, 919, 1159, 1191, 1704, 1741
BspMI ACCTGC 1 cut(s) 521
BspQI GCTCTTC 1 cut(s) 1353
BspT104I TTCGAA 1 cut(s) 415
BspTI CTTAAG 1 cut(s) 245
BsrBI CCGCTC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 1662
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 1 cut(s) 1190
BssECI CCNNGG 3 cut(s) 67, 124, 864
BssMI GATC 3 cut(s) 224, 1052, 1618
BssSI CACGAG 1 cut(s) 821
BssT1I CCWWGG 1 cut(s) 67
Bst2BI CACGAG 1 cut(s) 821
Bst4CI ACNGT 4 cut(s) 303, 556, 920, 1603
Bst6I CTCTTC 1 cut(s) 1353
BstAFI CTTAAG 1 cut(s) 245
BstAUI TGTACA 1 cut(s) 684
BstBI TTCGAA 1 cut(s) 415
BstC8I GCNNGC 2 cut(s) 1482, 1759
BstDEI CTNAG 8 cut(s) 221, 239, 510, 906, 1167, 1178, 1712, 1749
BstF5I GGATG 6 cut(s) 579, 658, 845, 1126, 1303, 1780
BstH2I RGCGCY 1 cut(s) 929
BstHHI GCGC 1 cut(s) 928
BstKTI GATC 3 cut(s) 227, 1055, 1621
BstMAI GTCTC 1 cut(s) 611
BstMBI GATC 3 cut(s) 224, 1052, 1618
BstMWI GCNNNNNNNGC 1 cut(s) 1477
BstNSI RCATGY 4 cut(s) 274, 394, 469, 1484
BstSLI GKGCMC 1 cut(s) 822
BstV1I GCAGC 2 cut(s) 847, 1496
BstX2I RGATCY 1 cut(s) 224
BstXI CCANNNNNNTGG 1 cut(s) 557
BstYI RGATCY 1 cut(s) 224
BsuRI GGCC 2 cut(s) 72, 116
BtsCI GGATG 6 cut(s) 579, 658, 845, 1126, 1303, 1780
BtsIMutI CAGTG 1 cut(s) 299
BveI ACCTGC 1 cut(s) 521
Cac8I GCNNGC 2 cut(s) 1482, 1759
CaiI CAGNNNCTG 1 cut(s) 1711
CfoI GCGC 1 cut(s) 928
Cfr13I GGNCC 4 cut(s) 79, 115, 1316, 1660
Csp6I GTAC 4 cut(s) 403, 685, 1149, 1299
CviQI GTAC 4 cut(s) 403, 685, 1149, 1299
DdeI CTNAG 8 cut(s) 221, 239, 510, 906, 1167, 1178, 1712, 1749
DpnI GATC 3 cut(s) 226, 1054, 1620
DpnII GATC 3 cut(s) 224, 1052, 1618
DraIII CACNNNGTG 1 cut(s) 1406
Eam1104I CTCTTC 1 cut(s) 1353
EarI CTCTTC 1 cut(s) 1353
Eco130I CCWWGG 1 cut(s) 67
Eco32I GATATC 2 cut(s) 933, 1423
Eco47I GGWCC 3 cut(s) 79, 1316, 1660
Eco47III AGCGCT 1 cut(s) 927
Eco57I CTGAAG 1 cut(s) 26
EcoRV GATATC 2 cut(s) 933, 1423
EcoT14I CCWWGG 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 67
FalI AAGNNNNNCTT 2 cut(s) 1125, 1157
FaqI GGGAC 1 cut(s) 51
FauI CCCGC 2 cut(s) 156, 427
FblI GTMKAC 1 cut(s) 1547
Fnu4HI GCNGC 3 cut(s) 861, 1485, 1793
FokI GGATG 6 cut(s) 586, 665, 832, 1113, 1290, 1767
Fsp4HI GCNGC 3 cut(s) 861, 1485, 1793
FspBI CTAG 4 cut(s) 68, 321, 648, 1040
GlaI GCGC 1 cut(s) 927
GluI GCNGC 3 cut(s) 861, 1485, 1793
GsaI CCCAGC 1 cut(s) 1538
HaeII RGCGCY 1 cut(s) 929
HaeIII GGCC 2 cut(s) 72, 116
HhaI GCGC 1 cut(s) 928
Hin6I GCGC 1 cut(s) 926
HinP1I GCGC 1 cut(s) 926
HincII GTYRAC 3 cut(s) 268, 579, 1548
HindII GTYRAC 3 cut(s) 268, 579, 1548
HinfI GANTC 9 cut(s) 169, 212, 564, 587, 592, 869, 1388, 1544, 1691
HphI GGTGA 1 cut(s) 959
Hpy166II GTNNAC 5 cut(s) 164, 268, 403, 579, 1548
Hpy188III TCNNGA 6 cut(s) 238, 964, 1050, 1130, 1418, 1433
Hpy8I GTNNAC 5 cut(s) 164, 268, 403, 579, 1548
HpyAV CCTTC 4 cut(s) 473, 799, 1430, 1512
HpyCH4III ACNGT 4 cut(s) 303, 556, 920, 1603
HpyCH4IV ACGT 1 cut(s) 1021
HpyF10VI GCNNNNNNNGC 1 cut(s) 1477
HpyF3I CTNAG 8 cut(s) 221, 239, 510, 906, 1167, 1178, 1712, 1749
HpySE526I ACGT 1 cut(s) 1021
HspAI GCGC 1 cut(s) 926
Kzo9I GATC 3 cut(s) 224, 1052, 1618
LguI GCTCTTC 1 cut(s) 1353
LmnI GCTCC 3 cut(s) 431, 1702, 1800
Lsp1109I GCAGC 2 cut(s) 847, 1496
LweI GCATC 2 cut(s) 1135, 1312
MaeI CTAG 4 cut(s) 68, 321, 648, 1040
MaeII ACGT 1 cut(s) 1021
MaeIII GTNAC 7 cut(s) 98, 303, 947, 1382, 1406, 1491, 1568
MalI GATC 3 cut(s) 226, 1054, 1620
MbiI CCGCTC 1 cut(s) 434
MboI GATC 3 cut(s) 224, 1052, 1618
MboII GAAGA 6 cut(s) 19, 754, 864, 867, 1256, 1340
MfeI CAATTG 1 cut(s) 972
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 1 cut(s) 822
MluCI AATT 7 cut(s) 417, 972, 1089, 1244, 1282, 1496, 1585
MlyI GAGTC 4 cut(s) 573, 586, 1553, 1685
MmeI TCCRAC 2 cut(s) 606, 699
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 4 cut(s) 246, 461, 632, 1239
MslI CAYNNNNRTG 1 cut(s) 945
MspA1I CMGCKG 1 cut(s) 860
MspCI CTTAAG 1 cut(s) 245
MunI CAATTG 1 cut(s) 972
Mva1269I GAATGC 2 cut(s) 1759, 1794
MwoI GCNNNNNNNGC 1 cut(s) 1477
NdeII GATC 3 cut(s) 224, 1052, 1618
NmeAIII GCCGAG 1 cut(s) 105
NmuCI GTSAC 5 cut(s) 98, 947, 1382, 1406, 1491
NspI RCATGY 4 cut(s) 274, 394, 469, 1484
NspV TTCGAA 1 cut(s) 415
OliI CACNNNNGTG 1 cut(s) 945
PaeI GCATGC 1 cut(s) 1484
PciI ACATGT 1 cut(s) 465
PciSI GCTCTTC 1 cut(s) 1353
PctI GAATGC 2 cut(s) 1759, 1794
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 5 cut(s) 169, 212, 587, 869, 1388
PflMI CCANNNNNTGG 1 cut(s) 1325
PkrI GCNGC 3 cut(s) 862, 1486, 1794
PleI GAGTC 4 cut(s) 572, 586, 1552, 1685
PpsI GAGTC 4 cut(s) 572, 586, 1552, 1685
PscI ACATGT 1 cut(s) 465
PspFI CCCAGC 1 cut(s) 1534
PspPI GGNCC 4 cut(s) 79, 115, 1316, 1660
PstNI CAGNNNCTG 1 cut(s) 1711
PsuI RGATCY 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 860
RsaI GTAC 4 cut(s) 404, 686, 1150, 1300
RsaNI GTAC 4 cut(s) 403, 685, 1149, 1299
RseI CAYNNNNRTG 1 cut(s) 945
SalI GTCGAC 1 cut(s) 1546
SapI GCTCTTC 1 cut(s) 1353
SaqAI TTAA 4 cut(s) 246, 461, 632, 1239
SatI GCNGC 3 cut(s) 861, 1485, 1793
Sau3AI GATC 3 cut(s) 224, 1052, 1618
Sau96I GGNCC 4 cut(s) 79, 115, 1316, 1660
SchI GAGTC 4 cut(s) 573, 586, 1553, 1685
SduI GDGCHC 1 cut(s) 822
SfaNI GCATC 2 cut(s) 1135, 1312
SfuI TTCGAA 1 cut(s) 415
SinI GGWCC 3 cut(s) 79, 1316, 1660
SmiMI CAYNNNNRTG 1 cut(s) 945
SmlI CTYRAG 5 cut(s) 245, 308, 701, 955, 962
SmoI CTYRAG 5 cut(s) 245, 308, 701, 955, 962
SphI GCATGC 1 cut(s) 1484
Sse9I AATT 7 cut(s) 417, 972, 1089, 1244, 1282, 1496, 1585
SsiI CCGC 2 cut(s) 149, 434
SspMI CTAG 4 cut(s) 68, 321, 648, 1040
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 4 cut(s) 303, 556, 920, 1603
TaiI ACGT 1 cut(s) 1024
TaqI TCGA 5 cut(s) 415, 590, 1051, 1107, 1547
TaqII GACCGA 1 cut(s) 1425
TasI AATT 7 cut(s) 417, 972, 1089, 1244, 1282, 1496, 1585
TatI WGTACW 1 cut(s) 684
TfiI GAWTC 5 cut(s) 169, 212, 587, 869, 1388
Tru1I TTAA 4 cut(s) 246, 461, 632, 1239
Tru9I TTAA 4 cut(s) 246, 461, 632, 1239
TscAI CASTG 1 cut(s) 306
TseFI GTSAC 5 cut(s) 98, 947, 1382, 1406, 1491
TseI GCWGC 3 cut(s) 860, 1484, 1792
Tsp45I GTSAC 5 cut(s) 98, 947, 1382, 1406, 1491
TspDTI ATGAA 3 cut(s) 123, 670, 1627
TspGWI ACGGA 2 cut(s) 205, 444
TspRI CASTG 1 cut(s) 306
Van91I CCANNNNNTGG 1 cut(s) 1325
Vha464I CTTAAG 1 cut(s) 245
VpaK11BI GGWCC 3 cut(s) 79, 1316, 1660
XapI RAATTY 3 cut(s) 1244, 1282, 1585
XceI RCATGY 4 cut(s) 274, 394, 469, 1484
XmaJI CCTAGG 1 cut(s) 67
XmiI GTMKAC 1 cut(s) 1547
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 4 cut(s) 68, 321, 648, 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.