MD02G1020800.v1.1

Arogenate dehydrogenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003175
Physical Location & Seq
Forward (+)
1474781 .. 1477398
2618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1020800.v1.1.491

Sequence Viewer

Length: 2088 bp
ATGTTATCTCTCCGCCTCCACTACCCATCGCCACCCAAACCATATCTATCTCCCTCCTCCTTCGTAAACCCCCAAAGCCTCAACCTTTCCCCAAACCCAACAACCACCCGCCCTCTCCGCCGCAGCTCCCTCAAAATCCGATCCATCGACTCCACCCAATTCTACGACTACGAGTCCAAGCTCGCCGCCCAATTCCGCTACGCAAGCATGCTCAAAATCGCCGTCATTGGCTTCGGCAACTACGGCCAATTCCTCGCCGAGGCCCTCGTCGCGCAAGGCCACACCGTCCTCGCCCATTCCCGATCCGACTACTCCAAAACGGCGCAAGACCTCGGCGTTTCGTTCTTCTCCGACCCCCACGACCTCTGCGAGCAGCACCCGCAAGTCATCCTGCTATGCACCTCCATCATTTCCACCGAGCCCGTCCTCAAATCGCTGCCTCTCCAGCGCCTCCGCCGCAACACCTTGTTCGTCGACGTGCTCTCCGTCAAGGAGTTCGCCAAGGCGTTGCTGCTCAAGTATCTGCCTCCCCACTTCGACATTCTTTGCACCCACCCGATGTTCGGACCCCAGAGCGCCAAAAACGGGTGGAAAGGGCTTCCTTTCGTCTACGAAAAAGTCCGGATTGGGTCCGAAGAATCGCGGGTTTCGCGGTGTGATAAGTTTCTGAGCATTTTTGAGGGAGAAGGGTGCCGGATGGTGGAGATGAGCTGCGCCGAGCATGATAAGTACGCGGCGGAGTCTCAATTCATCACCCACACGGTTGGGAGAGTGTTGGGGATGTTGCACTTGGAGTCGACTCCGATCAATACGAAAGGGTATGAGACATTGTTGGATTTGGTGAAGAACACGGAGGGGGATAGCTTCGATTTGTATTACGGGCTGTTTATGTACAACAAGAATACCTTGGAGATGTTGGAGAGGTTAGACGTGGCTTTGGAGGCTATGAAGAAAAAGATTTTCGGGCTTCTGCACAATTCTGTGCGAGACCAGATCTTTGGCAATGCGGAGAAGGGAAGAACTTTGCAGGAGGATAATGCGAATCGGGCTCAGAATGGACCTGCATTGGCATCTTCTTCTAGAGCTTCGAGATCGCCGAAAATTGCTCGACCACATGACCAAGAAGCAGAGGTTTCTGATGAATCTAGGCTAGAGATTGCAATTGTCGGTTTTGGTAACTTTGGCCAGTTCCTTGCTAAGACTATTGTACGTCAAGGCCATAAGGTTCTAGCATTCTCTCGATCAGATTACTCTGAAGTGGCTCATAAACTAGGCGTTTCATACTTCTCTGATGTGGATGATTTATGTGAAGAACATCCTGACGTGATACTACTTTGTACGTCTATTCTTTCAACCGAGAAAGTTTTGCGGTCATTGCCCCTGCATAGGCTTAAGAGAAATACTTTATTTGTTGATGTTCTTTCAGTGAAAGAATTTCCAAGAAATCTCTTTCTTCAAACATTGCCGCCGGAGTTTGATATTCTATGCACGCATCCTATGTTCGGACCAGAAAGTGGTAAGAACGGGTGGAATGGTCTCTCTTTTGTTTATGATGAGGTCAGGATTGGAAGTGAAGAATCTAGGGTATCACGGTGTGAGCAATTTCTTGATATCTTTGCACGAGAAGGGTGCCGAATGGTGGAAATGTCTTGTGCAGAACATGATAAGCATGCAGCAGGCTCACAATTCATTACACACACCGTAGGAAGGATTTTGGAGAAATTGGGTTTGGAGTCGACACCAATCAACACAAAAGGTTACGAGACTCTGTTGAATTTGGTGGAGAATACAGCGGGAGATAGCTTTGATCTCTACTACGGCTTGTTCATGTACAATGTAAATGCAATGGAGCAGCTGAAGAGATTGGACATGGCATTTCAATCTTTAAGGAAGCAGCTTTTCGGGCGCTTGCATGGTTTTGTTCGGAAGCAAAATTTTGAGACGTCGGATAAGTCTCAAGATACGCAGGAACAACCGTTGTTGCCAGAACCATCCCAGAGTGATCAAAATTTTGGGAAGTTGGATGAGTCTCAAGTTACGCAGGAACAACCGTTGTTGCCAGAACCATCCGGAGTGAATCTGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00400 ko01100 ko01110 ko01230 map00400 map01100 map01110 map01230
Pfam Domains
Protein Families
F420_oxidored PDH

Protein Analysis

696

Amino Acids

78.47

Weight (kDa)

6.22

Isoelectric Point (pI)

49.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 62 - 159 7.8e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
F420_oxidored PF03807 72 - 161 2.9e-09 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 86 - 192 2.5e-08 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 258 - 331 2.7e-31 TYRAAT1-like, C-terminal domain
2-Hacid_dh_C PF02826 375 - 472 5.2e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
F420_oxidored PF03807 386 - 474 1e-09 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 399 - 509 1.6e-09 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 571 - 644 4e-35 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1946
Acc36I ACCTGC 1 cut(s) 1069
AccB1I GGYRCC 2 cut(s) 690, 1629
AccB7I CCANNNNNTGG 1 cut(s) 1514
AccI GTMKAC 4 cut(s) 474, 609, 797, 1736
AccII CGCG 4 cut(s) 272, 643, 652, 734
AccIII TCCGGA 2 cut(s) 621, 2069
AclWI GGATC 2 cut(s) 135, 297
AcoI YGGCCR 2 cut(s) 244, 1183
AcsI RAATTY 4 cut(s) 1433, 1774, 1933, 2008
AcuI CTGAAG 2 cut(s) 1275, 1877
AcyI GRCGYC 1 cut(s) 1943
AdeI CACNNNGTG 1 cut(s) 1595
AfaI GTAC 5 cut(s) 731, 893, 1209, 1339, 1832
AfiI CCNNNNNNNGG 9 cut(s) 259, 563, 585, 700, 1386, 1502, 1514, 1639, 1707
AflII CTTAAG 1 cut(s) 1391
AgsI TTSAA 4 cut(s) 1353, 1457, 1774, 1880
AhdI GACNNNNNGTC 1 cut(s) 172
AjiI CACGTC 3 cut(s) 478, 931, 1324
AjuI GAANNNNNNNTTGG 2 cut(s) 1712, 1744
AluBI AGCT 8 cut(s) 126, 181, 711, 864, 1085, 1803, 1855, 1897
AluI AGCT 8 cut(s) 126, 181, 711, 864, 1085, 1803, 1855, 1897
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 8 cut(s) 747, 818, 981, 1541, 1757, 1934, 1959, 2034
AlwI GGATC 2 cut(s) 135, 297
Aor13HI TCCGGA 2 cut(s) 621, 2069
AoxI GGCC 5 cut(s) 244, 261, 277, 1183, 1216
ApeKI GCWGC 8 cut(s) 123, 373, 436, 511, 711, 1673, 1852, 1894
ApoI RAATTY 4 cut(s) 1433, 1774, 1933, 2008
ArsI GACNNNNNNTTYG 4 cut(s) 207, 239, 980, 1012
AspLEI GCGC 6 cut(s) 274, 325, 450, 578, 716, 1908
AspS9I GGNCC 5 cut(s) 262, 566, 630, 1058, 1505
AsuHPI GGTGA 2 cut(s) 745, 853
AvaII GGWCC 4 cut(s) 566, 630, 1058, 1505
BalI TGGCCA 1 cut(s) 1185
BanI GGYRCC 2 cut(s) 690, 1629
BanII GRGCYC 2 cut(s) 423, 1051
BauI CACGAG 1 cut(s) 1620
Bbv12I GWGCWC 1 cut(s) 483
BbvI GCAGC 8 cut(s) 135, 385, 423, 498, 698, 1685, 1864, 1906
BccI CCATC 6 cut(s) 34, 152, 413, 691, 1999, 2074
BceAI ACGGC 4 cut(s) 206, 259, 336, 1834
BcgI CGANNNNNNTGC 2 cut(s) 1611, 1645
BclI TGATCA 1 cut(s) 2002
BcoDI GTCTC 8 cut(s) 747, 818, 981, 1541, 1757, 1934, 1959, 2034
BfaI CTAG 6 cut(s) 1080, 1146, 1151, 1229, 1271, 1581
BfoI RGCGCY 3 cut(s) 451, 579, 1909
BfrI CTTAAG 1 cut(s) 1391
BfuAI ACCTGC 1 cut(s) 1069
BglII AGATCT 1 cut(s) 993
Bme18I GGWCC 4 cut(s) 566, 630, 1058, 1505
BmeRI GACNNNNNGTC 1 cut(s) 172
BmgBI CACGTC 3 cut(s) 478, 931, 1324
BmgT120I GGNCC 5 cut(s) 262, 566, 630, 1058, 1505
BmiI GGNNCC 4 cut(s) 568, 631, 692, 1631
BmsI GCATC 2 cut(s) 1079, 1501
BpmI CTGGAG 1 cut(s) 428
BpuEI CTTGAG 3 cut(s) 500, 1941, 2016
BsaHI GRCGYC 1 cut(s) 1943
BsaI GGTCTC 2 cut(s) 981, 1541
BsaJI CCNNGG 4 cut(s) 258, 331, 501, 906
BsaWI WCCGGW 2 cut(s) 621, 2069
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 9 cut(s) 259, 563, 585, 700, 1386, 1502, 1514, 1639, 1707
Bse1I ACTGG 1 cut(s) 1186
Bse3DI GCAATG 4 cut(s) 1009, 1373, 1460, 1851
BseAI TCCGGA 2 cut(s) 621, 2069
BseDI CCNNGG 4 cut(s) 258, 331, 501, 906
BseGI GGATG 9 cut(s) 387, 702, 786, 1303, 1315, 1492, 1991, 2029, 2066
BseLI CCNNNNNNNGG 9 cut(s) 259, 563, 585, 700, 1386, 1502, 1514, 1639, 1707
BseMI GCAATG 4 cut(s) 1009, 1373, 1460, 1851
BseMII CTCAG 2 cut(s) 659, 1064
BseNI ACTGG 1 cut(s) 1186
BseRI GAGGAG 1 cut(s) 46
BseXI GCAGC 8 cut(s) 135, 385, 423, 498, 698, 1685, 1864, 1906
BsgI GTGCAG 3 cut(s) 956, 1674, 2066
Bsh1236I CGCG 4 cut(s) 272, 643, 652, 734
BshFI GGCC 5 cut(s) 246, 263, 279, 1185, 1218
BshNI GGYRCC 2 cut(s) 690, 1629
BsiHKAI GWGCWC 1 cut(s) 483
BsiSI CCGG 4 cut(s) 622, 694, 1469, 2070
BslI CCNNNNNNNGG 9 cut(s) 259, 563, 585, 700, 1386, 1502, 1514, 1639, 1707
BsmAI GTCTC 8 cut(s) 747, 818, 981, 1541, 1757, 1934, 1959, 2034
BsmBI CGTCTC 1 cut(s) 1934
BsmI GAATGC 1 cut(s) 1232
BsnI GGCC 5 cut(s) 246, 263, 279, 1185, 1218
Bso31I GGTCTC 2 cut(s) 981, 1541
Bsp1286I GDGCHC 3 cut(s) 423, 483, 1051
Bsp13I TCCGGA 2 cut(s) 621, 2069
Bsp1407I TGTACA 2 cut(s) 891, 1830
Bsp143I GATC 8 cut(s) 140, 302, 804, 993, 1091, 1241, 1807, 2002
BspANI GGCC 5 cut(s) 246, 263, 279, 1185, 1218
BspCNI CTCAG 2 cut(s) 660, 1063
BspEI TCCGGA 2 cut(s) 621, 2069
BspFNI CGCG 4 cut(s) 272, 643, 652, 734
BspLI GGNNCC 4 cut(s) 568, 631, 692, 1631
BspMI ACCTGC 1 cut(s) 1069
BspPI GGATC 2 cut(s) 135, 297
BspT107I GGYRCC 2 cut(s) 690, 1629
BspTI CTTAAG 1 cut(s) 1391
BspTNI GGTCTC 2 cut(s) 981, 1541
BsrDI GCAATG 4 cut(s) 1009, 1373, 1460, 1851
BsrGI TGTACA 2 cut(s) 891, 1830
BsrI ACTGG 1 cut(s) 1186
BssECI CCNNGG 4 cut(s) 258, 331, 501, 906
BssMI GATC 8 cut(s) 140, 302, 804, 993, 1091, 1241, 1807, 2002
BssNI GRCGYC 1 cut(s) 1943
BssSI CACGAG 1 cut(s) 1620
BssT1I CCWWGG 2 cut(s) 501, 906
Bst2BI CACGAG 1 cut(s) 1620
Bst4CI ACNGT 6 cut(s) 286, 763, 1593, 1702, 1977, 2052
Bst6I CTCTTC 1 cut(s) 1853
BstACI GRCGYC 1 cut(s) 1943
BstAFI CTTAAG 1 cut(s) 1391
BstAUI TGTACA 2 cut(s) 891, 1830
BstC8I GCNNGC 8 cut(s) 183, 205, 209, 371, 1490, 1671, 1678, 1910
BstDEI CTNAG 3 cut(s) 668, 1050, 1197
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 9 cut(s) 387, 702, 786, 1303, 1315, 1492, 1991, 2029, 2066
BstFNI CGCG 4 cut(s) 272, 643, 652, 734
BstH2I RGCGCY 3 cut(s) 451, 579, 1909
BstHHI GCGC 6 cut(s) 274, 325, 450, 578, 716, 1908
BstKTI GATC 8 cut(s) 143, 305, 807, 996, 1094, 1244, 1810, 2005
BstMAI GTCTC 8 cut(s) 747, 818, 981, 1541, 1757, 1934, 1959, 2034
BstMBI GATC 8 cut(s) 140, 302, 804, 993, 1091, 1241, 1807, 2002
BstNSI RCATGY 2 cut(s) 211, 1673
BstUI CGCG 4 cut(s) 272, 643, 652, 734
BstV1I GCAGC 8 cut(s) 135, 385, 423, 498, 698, 1685, 1864, 1906
BstX2I RGATCY 1 cut(s) 993
BstXI CCANNNNNNTGG 2 cut(s) 764, 998
BstYI RGATCY 1 cut(s) 993
BsuRI GGCC 5 cut(s) 246, 263, 279, 1185, 1218
BtgZI GCGATG 1 cut(s) 12
BtrI CACGTC 3 cut(s) 478, 931, 1324
BtsCI GGATG 9 cut(s) 387, 702, 786, 1303, 1315, 1492, 1991, 2029, 2066
BtsIMutI CAGTG 1 cut(s) 1431
BveI ACCTGC 1 cut(s) 1069
Cac8I GCNNGC 8 cut(s) 183, 205, 209, 371, 1490, 1671, 1678, 1910
CfoI GCGC 6 cut(s) 274, 325, 450, 578, 716, 1908
Cfr13I GGNCC 5 cut(s) 262, 566, 630, 1058, 1505
Csp6I GTAC 5 cut(s) 730, 892, 1208, 1338, 1831
CviAII CATG 8 cut(s) 208, 722, 1115, 1661, 1670, 1828, 1870, 1913
CviQI GTAC 5 cut(s) 730, 892, 1208, 1338, 1831
DdeI CTNAG 3 cut(s) 668, 1050, 1197
DpnI GATC 8 cut(s) 142, 304, 806, 995, 1093, 1243, 1809, 2004
DpnII GATC 8 cut(s) 140, 302, 804, 993, 1091, 1241, 1807, 2002
DraIII CACNNNGTG 1 cut(s) 1595
DriI GACNNNNNGTC 1 cut(s) 172
EaeI YGGCCR 2 cut(s) 244, 1183
Eam1104I CTCTTC 1 cut(s) 1853
Eam1105I GACNNNNNGTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 1853
EciI GGCGGA 3 cut(s) 107, 443, 752
Eco130I CCWWGG 2 cut(s) 501, 906
Eco24I GRGCYC 2 cut(s) 423, 1051
Eco31I GGTCTC 2 cut(s) 981, 1541
Eco32I GATATC 1 cut(s) 1612
Eco47I GGWCC 4 cut(s) 566, 630, 1058, 1505
Eco57I CTGAAG 2 cut(s) 1275, 1877
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 262
EcoRV GATATC 1 cut(s) 1612
EcoT14I CCWWGG 2 cut(s) 501, 906
EcoT38I GRGCYC 2 cut(s) 423, 1051
ErhI CCWWGG 2 cut(s) 501, 906
Esp3I CGTCTC 1 cut(s) 1934
FaeI CATG 8 cut(s) 211, 725, 1118, 1664, 1673, 1831, 1873, 1916
FalI AAGNNNNNCTT 2 cut(s) 890, 922
FatI CATG 8 cut(s) 207, 721, 1114, 1660, 1669, 1827, 1869, 1912
FauI CCCGC 4 cut(s) 116, 387, 636, 1786
FbaI TGATCA 1 cut(s) 2002
FblI GTMKAC 4 cut(s) 474, 609, 797, 1736
FokI GGATG 9 cut(s) 374, 709, 793, 1302, 1310, 1479, 1978, 2036, 2053
FriOI GRGCYC 2 cut(s) 423, 1051
FspBI CTAG 6 cut(s) 1080, 1146, 1151, 1229, 1271, 1581
GlaI GCGC 6 cut(s) 273, 324, 449, 577, 715, 1907
GsuI CTGGAG 1 cut(s) 428
HaeII RGCGCY 3 cut(s) 451, 579, 1909
HaeIII GGCC 5 cut(s) 246, 263, 279, 1185, 1218
HapII CCGG 4 cut(s) 622, 694, 1469, 2070
HhaI GCGC 6 cut(s) 274, 325, 450, 578, 716, 1908
Hin1I GRCGYC 1 cut(s) 1943
Hin1II CATG 8 cut(s) 211, 725, 1118, 1664, 1673, 1831, 1873, 1916
Hin6I GCGC 6 cut(s) 272, 323, 448, 576, 714, 1906
HinP1I GCGC 6 cut(s) 272, 323, 448, 576, 714, 1906
HincII GTYRAC 3 cut(s) 475, 798, 1737
HindII GTYRAC 3 cut(s) 475, 798, 1737
HpaII CCGG 4 cut(s) 622, 694, 1469, 2070
HphI GGTGA 2 cut(s) 745, 853
Hpy166II GTNNAC 5 cut(s) 67, 475, 610, 798, 1737
Hpy8I GTNNAC 5 cut(s) 67, 475, 610, 798, 1737
Hpy99I CGWCG 4 cut(s) 272, 476, 479, 1948
HpyAV CCTTC 5 cut(s) 70, 680, 1006, 1619, 1701
HpyCH4III ACNGT 6 cut(s) 286, 763, 1593, 1702, 1977, 2052
HpyCH4IV ACGT 6 cut(s) 477, 930, 1210, 1323, 1340, 1943
HpyF3I CTNAG 3 cut(s) 668, 1050, 1197
HpySE526I ACGT 6 cut(s) 477, 930, 1210, 1323, 1340, 1943
Hsp92I GRCGYC 1 cut(s) 1943
Hsp92II CATG 8 cut(s) 211, 725, 1118, 1664, 1673, 1831, 1873, 1916
HspAI GCGC 6 cut(s) 272, 323, 448, 576, 714, 1906
Kpn2I TCCGGA 2 cut(s) 621, 2069
Ksp22I TGATCA 1 cut(s) 2002
Kzo9I GATC 8 cut(s) 140, 302, 804, 993, 1091, 1241, 1807, 2002
LmnI GCTCC 2 cut(s) 131, 1849
Lsp1109I GCAGC 8 cut(s) 135, 385, 423, 498, 698, 1685, 1864, 1906
LweI GCATC 2 cut(s) 1079, 1501
MaeI CTAG 6 cut(s) 1080, 1146, 1151, 1229, 1271, 1581
MaeII ACGT 6 cut(s) 477, 930, 1210, 1323, 1340, 1943
MaeIII GTNAC 3 cut(s) 1175, 1757, 2035
MalI GATC 8 cut(s) 142, 304, 806, 995, 1093, 1243, 1809, 2004
MboI GATC 8 cut(s) 140, 302, 804, 993, 1091, 1241, 1807, 2002
MfeI CAATTG 1 cut(s) 1161
MflI RGATCY 1 cut(s) 993
MhlI GDGCHC 3 cut(s) 423, 483, 1051
MlsI TGGCCA 1 cut(s) 1185
MluNI TGGCCA 1 cut(s) 1185
MlyI GAGTC 8 cut(s) 143, 182, 749, 793, 803, 1742, 1759, 2036
MmeI TCCRAC 6 cut(s) 330, 375, 813, 897, 1926, 2001
Mox20I TGGCCA 1 cut(s) 1185
MroI TCCGGA 2 cut(s) 621, 2069
MscI TGGCCA 1 cut(s) 1185
MseI TTAA 2 cut(s) 1392, 1886
Msp20I TGGCCA 1 cut(s) 1185
MspA1I CMGCKG 2 cut(s) 1793, 1855
MspCI CTTAAG 1 cut(s) 1391
MspI CCGG 4 cut(s) 622, 694, 1469, 2070
MunI CAATTG 1 cut(s) 1161
Mva1269I GAATGC 1 cut(s) 1232
MvnI CGCG 4 cut(s) 272, 643, 652, 734
NdeII GATC 8 cut(s) 140, 302, 804, 993, 1091, 1241, 1807, 2002
NlaIII CATG 8 cut(s) 211, 725, 1118, 1664, 1673, 1831, 1873, 1916
NlaIV GGNNCC 4 cut(s) 568, 631, 692, 1631
NmeAIII GCCGAG 3 cut(s) 283, 312, 742
NspI RCATGY 2 cut(s) 211, 1673
PaeI GCATGC 2 cut(s) 211, 1673
PcsI WCGNNNNNNNCGW 3 cut(s) 366, 483, 1094
PctI GAATGC 1 cut(s) 1232
PfeI GAWTC 5 cut(s) 638, 1042, 1142, 1577, 2077
PflMI CCANNNNNTGG 1 cut(s) 1514
PleI GAGTC 8 cut(s) 143, 181, 748, 793, 802, 1741, 1759, 2035
PpsI GAGTC 8 cut(s) 143, 181, 748, 793, 802, 1741, 1759, 2035
PspN4I GGNNCC 4 cut(s) 568, 631, 692, 1631
PspPI GGNCC 5 cut(s) 262, 566, 630, 1058, 1505
PsuI RGATCY 1 cut(s) 993
PvuII CAGCTG 1 cut(s) 1855
RsaI GTAC 5 cut(s) 731, 893, 1209, 1339, 1832
RsaNI GTAC 5 cut(s) 730, 892, 1208, 1338, 1831
SalI GTCGAC 3 cut(s) 473, 796, 1735
SaqAI TTAA 2 cut(s) 1392, 1886
Sau3AI GATC 8 cut(s) 140, 302, 804, 993, 1091, 1241, 1807, 2002
Sau96I GGNCC 5 cut(s) 262, 566, 630, 1058, 1505
SchI GAGTC 8 cut(s) 143, 182, 749, 793, 803, 1742, 1759, 2036
SduI GDGCHC 3 cut(s) 423, 483, 1051
SfaNI GCATC 2 cut(s) 1079, 1501
SgrDI CGTCGACG 1 cut(s) 473
SinI GGWCC 4 cut(s) 566, 630, 1058, 1505
SmlI CTYRAG 4 cut(s) 515, 1391, 1956, 2031
SmoI CTYRAG 4 cut(s) 515, 1391, 1956, 2031
SphI GCATGC 2 cut(s) 211, 1673
SspMI CTAG 6 cut(s) 1080, 1146, 1151, 1229, 1271, 1581
StyI CCWWGG 2 cut(s) 501, 906
TaaI ACNGT 6 cut(s) 286, 763, 1593, 1702, 1977, 2052
TaiI ACGT 6 cut(s) 480, 933, 1213, 1326, 1343, 1946
TaqI TCGA 9 cut(s) 147, 474, 537, 797, 867, 1088, 1108, 1240, 1736
TatI WGTACW 2 cut(s) 891, 1830
TauI GCSGC 5 cut(s) 123, 188, 459, 737, 1468
TfiI GAWTC 5 cut(s) 638, 1042, 1142, 1577, 2077
Tru1I TTAA 2 cut(s) 1392, 1886
Tru9I TTAA 2 cut(s) 1392, 1886
TscAI CASTG 1 cut(s) 1431
TseI GCWGC 8 cut(s) 123, 373, 436, 511, 711, 1673, 1852, 1894
TspDTI ATGAA 6 cut(s) 739, 962, 1155, 1269, 1678, 1816
TspGWI ACGGA 2 cut(s) 475, 866
TspRI CASTG 1 cut(s) 1431
Van91I CCANNNNNTGG 1 cut(s) 1514
Vha464I CTTAAG 1 cut(s) 1391
VpaK11BI GGWCC 4 cut(s) 566, 630, 1058, 1505
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 4 cut(s) 1433, 1774, 1933, 2008
XbaI TCTAGA 1 cut(s) 1079
XceI RCATGY 2 cut(s) 211, 1673
XmiI GTMKAC 4 cut(s) 474, 609, 797, 1736
XspI CTAG 6 cut(s) 1080, 1146, 1151, 1229, 1271, 1581
ZraI GACGTC 1 cut(s) 1944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.