Rroxscaffold_2G00153760

Arogenate dehydrogenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003175
Physical Location & Seq
Forward (+)
90490590 .. 90493512
2923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00153760.1

Sequence Viewer

Length: 1329 bp
ATGCTATCCCTCTCACCTCTCCACCCATTGCCTCCAAAACTCATCTCCACTCGCTCTCCCTTCTTCAACCCCCAAAGCCCCAACCTTTACCCCCAAAGCTCCAACCTTTCCCCCAAAATCCCTAACCTTTCCCCAAAACGACGCCGTTCCCTCAAAATAAAATCCCTAGACGCCGCCCAATTATTCGACTATGAAACCAAGCTGGCCGCCCAGTACCACTCCTCCCAAACCCTCAAGATCGCCATCATCGGCTTCGGCAACTTCGGCCAATTCCTCGCCAAAACCCTAACCCGCCAAGGCCACACCGTCCTCGCCTATTCCCGCTCCGACCACTCCCAAACCGCAGCCGCCCTCGGCGTTTCCTTCTTCTCCGACCCCCACGACCTCTGCGAGCAGCACCCCCACGTCATCCTCCTCTGCACCTCCATCATCTCCGCCGAGAAAGTCCTCAACTCCCTCCCCCTCCAGCGCCTCCGCCGCAACACTCTCTTCGCCGACGTCCTCTCCGTCAAGGAGTTCTCCAAAGCTCTGTTCTTGAAATCTCTTCCGAGCTATTTCGATGTGATCTGCACGCACCCGATGTTCGGGCCGGAGAGCGCCAAGAACGGCTGGAATGGGCTGTGCTTTGTTTACGAGAAAGTGAGGATTGGATCGGAGGTGTCTAGAGTTTCGAGGTGTGACAAGTTCTTGAGCATTTTCGAGAGGGAAGGGTGTGAAATGGTGGAAATGAGCTGTGCCGAGCATGATAAGTTCGCGGCGGGGTCGCAGTTTATTACACACACAGTTGGGAGGGTGCTTGAGATGTTGAGGTTGGAGTCGACGCCGATTAATACTAGAGGGTATGAGACATTGTTGGATTTGGTGGAGAATACGGCTGGGGATAGCTTTGATTTGTATTATGGGTTGTTTATGTATAATAAGAATGCGTTGGAGATGCTGGAGAGGTTGGACTTGGCTTTCGAGGATTTGAAGAAGCAGCTTTTCGGGAGGTTGCATGATGTTGTGAGGAACCAGTTGTTTGGGAATGCCGAAAAGGTGAGGACGTTGCAGGAGGAGTATGTGAATCAGAATGGAGCTGCATTGGTGCGTTCTTCGAAAGCTGTGAGATCCTCTAAAGCACATATTTTGGATAGCTCCGAAGATCATTCAAGGCTTAAGATTGGAATTGTTGGTTTTGGCAACTTTGGTCAGTTCCTTGCTAAAACCATTGTACGCCAAGGCCACAAGGTTCTAGCCTATTCTCGCTCAGACTACTCTGATGTTGCTCAACAGTTGGGTGTTTCTTACTTCTCCGATGCGGATGATTTTGCGAGGAGCATCCAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00400 ko01100 ko01110 ko01230 map00400 map01100 map01110 map01230
Pfam Domains
Protein Families
F420_oxidored PDH

Protein Analysis

442

Amino Acids

49.68

Weight (kDa)

8.9

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 66 - 164 1.4e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
F420_oxidored PF03807 79 - 163 1.5e-09 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_2 PF03446 79 - 166 4e-07 NAD binding domain of 6-phosphogluconate dehydrogenase
PDH_N PF02153 92 - 199 3.3e-09 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 265 - 338 1.2e-35 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 501
AccBSI CCGCTC 1 cut(s) 324
AccI GTMKAC 1 cut(s) 818
AccII CGCG 1 cut(s) 755
AclWI GGATC 2 cut(s) 658, 1101
AcoI YGGCCR 2 cut(s) 204, 265
AcyI GRCGYC 4 cut(s) 142, 171, 498, 821
AfaI GTAC 2 cut(s) 215, 1212
AfiI CCNNNNNNNGG 1 cut(s) 584
AflII CTTAAG 1 cut(s) 1154
AgsI TTSAA 4 cut(s) 67, 538, 970, 1149
AjiI CACGTC 1 cut(s) 406
AjuI GAANNNNNNNTTGG 2 cut(s) 515, 547
Alw26I GTCTC 1 cut(s) 839
AlwI GGATC 2 cut(s) 658, 1101
AoxI GGCC 5 cut(s) 204, 265, 298, 587, 1219
ApeKI GCWGC 4 cut(s) 344, 394, 976, 1076
ArsI GACNNNNNNTTYG 2 cut(s) 941, 973
AseI ATTAAT 1 cut(s) 828
AspLEI GCGC 2 cut(s) 471, 599
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 2 cut(s) 6, 1048
AsuII TTCGAA 1 cut(s) 1094
BbvI GCAGC 4 cut(s) 356, 406, 988, 1063
BccI CCATC 2 cut(s) 251, 434
BceAI ACGGC 3 cut(s) 129, 622, 888
BcoDI GTCTC 1 cut(s) 839
BfaI CTAG 4 cut(s) 167, 663, 834, 1232
BfoI RGCGCY 2 cut(s) 472, 600
BfrI CTTAAG 1 cut(s) 1154
BisI GCNGC 9 cut(s) 174, 207, 345, 348, 395, 478, 756, 977, 1077
BlsI GCNGC 9 cut(s) 175, 208, 346, 349, 396, 479, 757, 978, 1078
BmgBI CACGTC 1 cut(s) 406
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 1 cut(s) 1010
BmrI ACTGGG 1 cut(s) 205
BmsI GCATC 2 cut(s) 924, 1285
BmuI ACTGGG 1 cut(s) 205
BpmI CTGGAG 2 cut(s) 449, 959
Bpu14I TTCGAA 1 cut(s) 1094
BpuEI CTTGAG 3 cut(s) 218, 709, 818
BsaBI GATNNNNATC 1 cut(s) 242
BsaHI GRCGYC 4 cut(s) 142, 171, 498, 821
BsaJI CCNNGG 3 cut(s) 295, 352, 1216
BsaXI ACNNNNNCTCC 6 cut(s) 40, 70, 206, 236, 488, 518
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse1I ACTGG 2 cut(s) 211, 1012
Bse3DI GCAATG 1 cut(s) 26
Bse8I GATNNNNATC 1 cut(s) 242
BseDI CCNNGG 3 cut(s) 295, 352, 1216
BseGI GGATG 3 cut(s) 408, 1306, 1317
BseJI GATNNNNATC 1 cut(s) 242
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMI GCAATG 1 cut(s) 26
BseMII CTCAG 1 cut(s) 1260
BseNI ACTGG 2 cut(s) 211, 1012
BseRI GAGGAG 4 cut(s) 211, 404, 1067, 1327
BseXI GCAGC 4 cut(s) 356, 406, 988, 1063
BseYI CCCAGC 1 cut(s) 875
BsgI GTGCAG 2 cut(s) 403, 553
Bsh1236I CGCG 1 cut(s) 755
BshFI GGCC 5 cut(s) 206, 267, 300, 589, 1221
BsiSI CCGG 1 cut(s) 590
BslI CCNNNNNNNGG 1 cut(s) 584
BsmAI GTCTC 1 cut(s) 839
BsmI GAATGC 2 cut(s) 928, 1030
BsnI GGCC 5 cut(s) 206, 267, 300, 589, 1221
Bsp119I TTCGAA 1 cut(s) 1094
Bsp143I GATC 5 cut(s) 237, 564, 650, 1106, 1141
BspANI GGCC 5 cut(s) 206, 267, 300, 589, 1221
BspCNI CTCAG 1 cut(s) 1259
BspFNI CGCG 1 cut(s) 755
BspLI GGNNCC 1 cut(s) 1010
BspPI GGATC 2 cut(s) 658, 1101
BspT104I TTCGAA 1 cut(s) 1094
BspTI CTTAAG 1 cut(s) 1154
BsrBI CCGCTC 1 cut(s) 324
BsrDI GCAATG 1 cut(s) 26
BsrI ACTGG 2 cut(s) 211, 1012
BssECI CCNNGG 3 cut(s) 295, 352, 1216
BssMI GATC 5 cut(s) 237, 564, 650, 1106, 1141
BssNI GRCGYC 4 cut(s) 142, 171, 498, 821
BssT1I CCWWGG 2 cut(s) 295, 1216
Bst4CI ACNGT 3 cut(s) 307, 784, 1272
Bst6I CTCTTC 2 cut(s) 494, 549
BstACI GRCGYC 4 cut(s) 142, 171, 498, 821
BstAFI CTTAAG 1 cut(s) 1154
BstBI TTCGAA 1 cut(s) 1094
BstC8I GCNNGC 3 cut(s) 204, 392, 572
BstDEI CTNAG 1 cut(s) 1246
BstF5I GGATG 3 cut(s) 408, 1306, 1317
BstFNI CGCG 1 cut(s) 755
BstH2I RGCGCY 2 cut(s) 472, 600
BstHHI GCGC 2 cut(s) 471, 599
BstKTI GATC 5 cut(s) 240, 567, 653, 1109, 1144
BstMAI GTCTC 1 cut(s) 839
BstMBI GATC 5 cut(s) 237, 564, 650, 1106, 1141
BstMWI GCNNNNNNNGC 2 cut(s) 264, 477
BstUI CGCG 1 cut(s) 755
BstV1I GCAGC 4 cut(s) 356, 406, 988, 1063
BstX2I RGATCY 1 cut(s) 1106
BstXI CCANNNNNNTGG 1 cut(s) 1019
BstYI RGATCY 1 cut(s) 1106
BsuRI GGCC 5 cut(s) 206, 267, 300, 589, 1221
BtrI CACGTC 1 cut(s) 406
BtsCI GGATG 3 cut(s) 408, 1306, 1317
Cac8I GCNNGC 3 cut(s) 204, 392, 572
CfoI GCGC 2 cut(s) 471, 599
Cfr13I GGNCC 1 cut(s) 587
CseI GACGC 3 cut(s) 150, 179, 829
Csp6I GTAC 2 cut(s) 214, 1211
CviAII CATG 2 cut(s) 743, 995
CviQI GTAC 2 cut(s) 214, 1211
DdeI CTNAG 1 cut(s) 1246
DpnI GATC 5 cut(s) 239, 566, 652, 1108, 1143
DpnII GATC 5 cut(s) 237, 564, 650, 1106, 1141
EaeI YGGCCR 2 cut(s) 204, 265
Eam1104I CTCTTC 2 cut(s) 494, 549
EarI CTCTTC 2 cut(s) 494, 549
EciI GGCGGA 2 cut(s) 424, 464
Eco130I CCWWGG 2 cut(s) 295, 1216
EcoT14I CCWWGG 2 cut(s) 295, 1216
ErhI CCWWGG 2 cut(s) 295, 1216
FaeI CATG 2 cut(s) 746, 998
FaiI YATR 9 cut(s) 192, 744, 843, 900, 911, 915, 996, 1059, 1122
FatI CATG 2 cut(s) 742, 994
FauI CCCGC 3 cut(s) 299, 329, 751
FblI GTMKAC 1 cut(s) 818
Fnu4HI GCNGC 9 cut(s) 174, 207, 345, 348, 395, 478, 756, 977, 1077
FokI GGATG 3 cut(s) 395, 1304, 1313
Fsp4HI GCNGC 9 cut(s) 174, 207, 345, 348, 395, 478, 756, 977, 1077
FspBI CTAG 4 cut(s) 167, 663, 834, 1232
GlaI GCGC 2 cut(s) 470, 598
GluI GCNGC 9 cut(s) 174, 207, 345, 348, 395, 478, 756, 977, 1077
GsaI CCCAGC 1 cut(s) 879
GsuI CTGGAG 2 cut(s) 449, 959
HaeII RGCGCY 2 cut(s) 472, 600
HaeIII GGCC 5 cut(s) 206, 267, 300, 589, 1221
HapII CCGG 1 cut(s) 590
HgaI GACGC 3 cut(s) 150, 179, 829
HhaI GCGC 2 cut(s) 471, 599
Hin1I GRCGYC 4 cut(s) 142, 171, 498, 821
Hin1II CATG 2 cut(s) 746, 998
Hin6I GCGC 2 cut(s) 469, 597
HinP1I GCGC 2 cut(s) 469, 597
HincII GTYRAC 1 cut(s) 819
HindII GTYRAC 1 cut(s) 819
HinfI GANTC 2 cut(s) 815, 1063
HpaII CCGG 1 cut(s) 590
HphI GGTGA 2 cut(s) 6, 1048
Hpy166II GTNNAC 2 cut(s) 631, 819
Hpy188I TCNGA 9 cut(s) 328, 373, 549, 655, 1068, 1138, 1249, 1258, 1294
Hpy188III TCNNGA 7 cut(s) 235, 535, 663, 688, 700, 985, 1321
Hpy8I GTNNAC 2 cut(s) 631, 819
Hpy99I CGWCG 3 cut(s) 144, 500, 823
HpyAV CCTTC 3 cut(s) 70, 373, 701
HpyCH4III ACNGT 3 cut(s) 307, 784, 1272
HpyCH4IV ACGT 3 cut(s) 405, 498, 1043
HpyCH4V TGCA 5 cut(s) 420, 570, 994, 1048, 1079
HpyF10VI GCNNNNNNNGC 2 cut(s) 264, 477
HpyF3I CTNAG 1 cut(s) 1246
HpySE526I ACGT 3 cut(s) 405, 498, 1043
Hsp92I GRCGYC 4 cut(s) 142, 171, 498, 821
Hsp92II CATG 2 cut(s) 746, 998
HspAI GCGC 2 cut(s) 469, 597
Kzo9I GATC 5 cut(s) 237, 564, 650, 1106, 1141
LmnI GCTCC 5 cut(s) 104, 329, 1073, 1139, 1314
LpnPI CCDG 9 cut(s) 188, 224, 479, 595, 603, 861, 923, 1025, 1034
Lsp1109I GCAGC 4 cut(s) 356, 406, 988, 1063
LweI GCATC 2 cut(s) 924, 1285
MaeI CTAG 4 cut(s) 167, 663, 834, 1232
MaeII ACGT 3 cut(s) 405, 498, 1043
MaeIII GTNAC 1 cut(s) 677
MalI GATC 5 cut(s) 239, 566, 652, 1108, 1143
MbiI CCGCTC 1 cut(s) 324
MboI GATC 5 cut(s) 237, 564, 650, 1106, 1141
MboII GAAGA 7 cut(s) 55, 358, 481, 536, 982, 1083, 1151
MflI RGATCY 1 cut(s) 1106
MluCI AATT 3 cut(s) 179, 269, 1164
MlyI GAGTC 1 cut(s) 824
MmeI TCCRAC 7 cut(s) 126, 351, 396, 792, 834, 909, 927
MseI TTAA 2 cut(s) 828, 1155
MspCI CTTAAG 1 cut(s) 1154
MspI CCGG 1 cut(s) 590
Mva1269I GAATGC 2 cut(s) 928, 1030
MvnI CGCG 1 cut(s) 755
MwoI GCNNNNNNNGC 2 cut(s) 264, 477
NdeII GATC 5 cut(s) 237, 564, 650, 1106, 1141
NlaIII CATG 2 cut(s) 746, 998
NlaIV GGNNCC 1 cut(s) 1010
NmeAIII GCCGAG 3 cut(s) 333, 463, 763
NmuCI GTSAC 1 cut(s) 677
NspV TTCGAA 1 cut(s) 1094
PcsI WCGNNNNNNNCGW 2 cut(s) 387, 504
PctI GAATGC 2 cut(s) 928, 1030
PfeI GAWTC 1 cut(s) 1063
PkrI GCNGC 9 cut(s) 175, 208, 346, 349, 396, 479, 757, 978, 1078
PleI GAGTC 1 cut(s) 823
PpsI GAGTC 1 cut(s) 823
PshBI ATTAAT 1 cut(s) 828
PspFI CCCAGC 1 cut(s) 875
PspN4I GGNNCC 1 cut(s) 1010
PspPI GGNCC 1 cut(s) 587
PsuI RGATCY 1 cut(s) 1106
RsaI GTAC 2 cut(s) 215, 1212
RsaNI GTAC 2 cut(s) 214, 1211
SalI GTCGAC 1 cut(s) 817
SaqAI TTAA 2 cut(s) 828, 1155
SatI GCNGC 9 cut(s) 174, 207, 345, 348, 395, 478, 756, 977, 1077
Sau3AI GATC 5 cut(s) 237, 564, 650, 1106, 1141
Sau96I GGNCC 1 cut(s) 587
SchI GAGTC 1 cut(s) 824
SfaNI GCATC 2 cut(s) 924, 1285
SfuI TTCGAA 1 cut(s) 1094
SmlI CTYRAG 4 cut(s) 233, 688, 797, 1154
SmoI CTYRAG 4 cut(s) 233, 688, 797, 1154
Sse9I AATT 3 cut(s) 179, 269, 1164
SspMI CTAG 4 cut(s) 167, 663, 834, 1232
StyI CCWWGG 2 cut(s) 295, 1216
TaaI ACNGT 3 cut(s) 307, 784, 1272
TaiI ACGT 3 cut(s) 408, 501, 1046
TaqI TCGA 7 cut(s) 186, 558, 671, 699, 818, 960, 1094
TasI AATT 3 cut(s) 179, 269, 1164
TauI GCSGC 5 cut(s) 176, 209, 350, 480, 758
TfiI GAWTC 1 cut(s) 1063
Tru1I TTAA 2 cut(s) 828, 1155
Tru9I TTAA 2 cut(s) 828, 1155
TseFI GTSAC 1 cut(s) 677
TseI GCWGC 4 cut(s) 344, 394, 976, 1076
Tsp45I GTSAC 1 cut(s) 677
TspDTI ATGAA 1 cut(s) 207
TspGWI ACGGA 1 cut(s) 496
Vha464I CTTAAG 1 cut(s) 1154
VspI ATTAAT 1 cut(s) 828
XbaI TCTAGA 1 cut(s) 662
XmiI GTMKAC 1 cut(s) 818
XspI CTAG 4 cut(s) 167, 663, 834, 1232
ZraI GACGTC 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.