Rw2G002140

Arogenate dehydrogenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003175
Physical Location & Seq
Reverse (-)
1786070 .. 1788283
2214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G002140.1

Sequence Viewer

Length: 1989 bp
ATGCTATCCCTCTCACCTCTCCACCCATTCCCTCCAAAACTCATCCTCACTCGCTCTCCCTTCTTCAACCCCCAAAGCCCCAACCTTTACCCCCAAAGCTCCAACCTTTCCCCCAAAATCCCTAACCTATCCCCAAAACGACGCCGTTTTCGTTCCCTCAAAATCAAATCCCTAGACGCCGCCCAATTATTCGACTACGAAACCAAGCTGGCCGCCCAGTACCAGTCCTCCCAAGCCCTCAAGATCGCCATCATCGGCTTCGGCAACTTCGGCCAATTCCTCGCCAAAACCCTAACCCGCCAAGGCCACACCGTCCTCGCCTATTCCCGCTCCGACCACTCCAAAGCGGCAGCCGCCCTCGGCGTTTCCTTCTTCCCCGACCCCCACGACCTCTGCGAGCAGCACCCCCACGTCATCCTCCTCTGCACCTCCATCATCTCCGCCGAGAAAGTAATCAACTCCCTCCCCCTCCAGCGCCTCCGCCGCAACACTCTCTTCGCCGACGTCCTCTCCGTCAAGGAGTTCTCCAAAGCTCTGTTCTTGAAGTCCCTTCCGAGCTATTTCGATGTGATCTGCACGCACCCGATGTTCGGGCCGGAGAGCGCGAAGAACGGCTGGAGCGGGCTGTGCTTTGTTTATGAGAAAGTGAGGATTGGATCGGAGGTGTCTAGAGTTTCGAGGTGTGACAAGTTCTTGAGCATTTTCGAGAGGGAAGGGTGTGAAATGGTGGAAATGAGCTGTGCCGAGCATGATAAGTTCGCGGCGGGGTCGCAGTTTATTACACACACTGTTGGGAGGGTGCTTGAGAGGTTGGGGTTGGAGTCGACGCCGATTAATACTAGAGGCTATGAGACATTGTTGGATTTGGTGGAGAATACGGCTGGGGATAGCTTTGATTTGTATTACGGGTTGTTTATGTATAATAAGAATGCGTTGGAGATGCTGGAGAGGTTGGACTTGGCTTTCGAGGATTTGAAGAAGCAGCTTTTCGGGAGGTTGCATGATGTTGTCAGGAACCAGTTGTTTGGGAATGCTGAAAAGGTGAGGACGTTGCAGGAGGAGTATGTGAACCAGAATGGAGCTGCATTGGTGCATTGGAATTGTTGGTTTGGCAACTTTGGTCAGTTCCTTGCTAAAACCATTGTACGCCAAGGCCACAAGGTTCTTGCCTATTCTCGCTCAGACTACTCTGATGTTGCTGAACAGTTGGGTGTTTCTTACTTCTCGGACGCGGATGATTTTTGCGAGGAGCATCCAGAAGTAATACTCCTTTGCACATCGATTCTTTCCACTGAGAAAGTTTTGATGTCACTCCCGCTTCAGAGGTTGAAGCGGAGTACTTTGTTTGTTGATGTTCTATCCGTGAAAGAATTCCCAAAAAACCTGTTTCTTCAAAATTTGCCTCTAGATTTTGATATTCTCTGCACGCATCCTATGTTTGGACCAGAAAGTGGTAAAAATGGCTGGAATGGTCTTGCTTTTGTTTATGATAGGATCAGAATTGGAAATGACGAATCAAGGGTGTCAAGGTGTGATCAATTTCTTGATATTTTTGCACAAGAAGGGTGTCGAATGGTGGATATGGCTTGCGAAGAGCATGATAAGCATGCAGCAGGGTCACAATTCATCACACACACTATGGGAAGAGTTTTGGAAAAGTTGGGTTTGGAGTCGACGCCGGTCAATACAAAAGGTTACAAGACTTTGTTGAATTTGGTGGAGAATACAGCAGGAGATAGCTTTGATCTCTACTATGGATTGTTCATGTACAATGTAAATGCAATGGAGCAGCTAAAGAGGCTTGACCTTGCATTTGAAGCATTAAAGAAACAGCTTTTTGGGCGGTTGCATGGTGTTCTTCGGAAGCAGCTTTTTGAGAATGCAGATGATTCTCAAGTTATGAAGGAGCAGCATTTGTTGCCAACACCATTGGAGAATGAATCTGCAGCGACACCTTCTTTCGAGGCTCTTAACAGTCAGAACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00400 ko01100 ko01110 ko01230 map00400 map01100 map01110 map01230
Pfam Domains
Protein Families
F420_oxidored PDH

Protein Analysis

662

Amino Acids

74.94

Weight (kDa)

6.36

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 69 - 166 1.4e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
F420_oxidored PF03807 81 - 165 3e-09 NADP oxidoreductase coenzyme F420-dependent
NAD_binding_2 PF03446 81 - 168 9.5e-07 NAD binding domain of 6-phosphogluconate dehydrogenase
PDH_N PF02153 94 - 201 1.9e-08 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 267 - 340 1.3e-35 TYRAAT1-like, C-terminal domain
F420_oxidored PF03807 370 - 453 5.9e-06 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 378 - 485 8.2e-09 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 550 - 623 3.9e-35 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 507
AccB7I CCANNNNNTGG 1 cut(s) 1451
AccBSI CCGCTC 2 cut(s) 330, 621
AccI GTMKAC 2 cut(s) 824, 1673
AccII CGCG 3 cut(s) 605, 761, 1232
AclWI GGATC 2 cut(s) 664, 1502
AcoI YGGCCR 2 cut(s) 210, 271
AcsI RAATTY 3 cut(s) 1370, 1396, 1711
AcuI CTGAAG 1 cut(s) 1304
AcyI GRCGYC 5 cut(s) 142, 177, 504, 827, 1676
AfaI GTAC 4 cut(s) 221, 1146, 1339, 1769
AfiI CCNNNNNNNGG 3 cut(s) 590, 1439, 1451
AgsI TTSAA 7 cut(s) 67, 544, 976, 1330, 1394, 1711, 1817
AjiI CACGTC 1 cut(s) 412
AjuI GAANNNNNNNTTGG 2 cut(s) 521, 553
Alw26I GTCTC 1 cut(s) 845
AlwI GGATC 2 cut(s) 664, 1502
AoxI GGCC 5 cut(s) 210, 271, 304, 593, 1153
ApeKI GCWGC 9 cut(s) 350, 400, 982, 1082, 1610, 1789, 1867, 1909, 1946
ApoI RAATTY 3 cut(s) 1370, 1396, 1711
ArsI GACNNNNNNTTYG 6 cut(s) 132, 164, 947, 979, 1796, 1828
AseI ATTAAT 1 cut(s) 834
Asp700I GAANNNNTTC 1 cut(s) 1370
AspLEI GCGC 2 cut(s) 477, 605
AspS9I GGNCC 2 cut(s) 593, 1442
AsuHPI GGTGA 2 cut(s) 6, 1054
AvaII GGWCC 1 cut(s) 1442
BbvI GCAGC 9 cut(s) 362, 412, 994, 1069, 1622, 1801, 1879, 1921, 1958
BccI CCATC 2 cut(s) 257, 440
BceAI ACGGC 3 cut(s) 129, 628, 894
BclI TGATCA 1 cut(s) 1534
BcoDI GTCTC 1 cut(s) 845
BfaI CTAG 4 cut(s) 173, 669, 840, 1406
BfmI CTRYAG 1 cut(s) 1944
BfoI RGCGCY 1 cut(s) 478
BmcAI AGTACT 1 cut(s) 1339
Bme18I GGWCC 1 cut(s) 1442
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 2 cut(s) 593, 1442
BmiI GGNNCC 1 cut(s) 1016
BmrI ACTGGG 1 cut(s) 211
BmsI GCATC 3 cut(s) 930, 1261, 1438
BmuI ACTGGG 1 cut(s) 211
BoxI GACNNNNGTC 1 cut(s) 1679
BpmI CTGGAG 3 cut(s) 455, 637, 965
BpuEI CTTGAG 4 cut(s) 224, 715, 824, 1878
Bsa29I ATCGAT 1 cut(s) 1280
BsaBI GATNNNNATC 1 cut(s) 248
BsaHI GRCGYC 5 cut(s) 142, 177, 504, 827, 1676
BsaJI CCNNGG 3 cut(s) 301, 358, 1150
BsaXI ACNNNNNCTCC 6 cut(s) 40, 70, 212, 242, 494, 524
Bsc4I CCNNNNNNNGG 3 cut(s) 590, 1439, 1451
Bse118I RCCGGY 1 cut(s) 1678
Bse1I ACTGG 3 cut(s) 217, 223, 1018
Bse3DI GCAATG 1 cut(s) 1788
Bse8I GATNNNNATC 1 cut(s) 248
BseCI ATCGAT 1 cut(s) 1280
BseDI CCNNGG 3 cut(s) 301, 358, 1150
BseGI GGATG 5 cut(s) 42, 414, 1240, 1252, 1429
BseJI GATNNNNATC 1 cut(s) 248
BseLI CCNNNNNNNGG 3 cut(s) 590, 1439, 1451
BseMI GCAATG 1 cut(s) 1788
BseMII CTCAG 2 cut(s) 1194, 1284
BseNI ACTGG 3 cut(s) 217, 223, 1018
BseRI GAGGAG 3 cut(s) 410, 1073, 1262
BseXI GCAGC 9 cut(s) 362, 412, 994, 1069, 1622, 1801, 1879, 1921, 1958
BseYI CCCAGC 1 cut(s) 881
BsgI GTGCAG 3 cut(s) 409, 559, 1408
Bsh1236I CGCG 3 cut(s) 605, 761, 1232
BshFI GGCC 5 cut(s) 212, 273, 306, 595, 1155
BshVI ATCGAT 1 cut(s) 1280
BsiSI CCGG 2 cut(s) 596, 1679
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 3 cut(s) 590, 1439, 1451
BsmAI GTCTC 1 cut(s) 845
BsmFI GGGAC 1 cut(s) 532
BsmI GAATGC 3 cut(s) 934, 1036, 1885
BsnI GGCC 5 cut(s) 212, 273, 306, 595, 1155
Bsp1407I TGTACA 1 cut(s) 1767
Bsp143I GATC 6 cut(s) 243, 570, 656, 1494, 1534, 1744
BspANI GGCC 5 cut(s) 212, 273, 306, 595, 1155
BspCNI CTCAG 2 cut(s) 1193, 1285
BspDI ATCGAT 1 cut(s) 1280
BspFNI CGCG 3 cut(s) 605, 761, 1232
BspLI GGNNCC 1 cut(s) 1016
BspMAI CTGCAG 1 cut(s) 1948
BspPI GGATC 2 cut(s) 664, 1502
BspQI GCTCTTC 1 cut(s) 1587
BsrBI CCGCTC 2 cut(s) 330, 621
BsrDI GCAATG 1 cut(s) 1788
BsrFI RCCGGY 1 cut(s) 1678
BsrGI TGTACA 1 cut(s) 1767
BsrI ACTGG 3 cut(s) 217, 223, 1018
BssAI RCCGGY 1 cut(s) 1678
BssECI CCNNGG 3 cut(s) 301, 358, 1150
BssMI GATC 6 cut(s) 243, 570, 656, 1494, 1534, 1744
BssNI GRCGYC 5 cut(s) 142, 177, 504, 827, 1676
BssT1I CCWWGG 2 cut(s) 301, 1150
Bst4CI ACNGT 4 cut(s) 313, 790, 1206, 1976
Bst6I CTCTTC 3 cut(s) 500, 1587, 1639
BstACI GRCGYC 5 cut(s) 142, 177, 504, 827, 1676
BstAPI GCANNNNNTGC 1 cut(s) 1918
BstAUI TGTACA 1 cut(s) 1767
BstC8I GCNNGC 7 cut(s) 210, 398, 578, 623, 1427, 1588, 1608
BstDEI CTNAG 2 cut(s) 1180, 1293
BstF5I GGATG 5 cut(s) 42, 414, 1240, 1252, 1429
BstFNI CGCG 3 cut(s) 605, 761, 1232
BstH2I RGCGCY 1 cut(s) 478
BstHHI GCGC 2 cut(s) 477, 605
BstKTI GATC 6 cut(s) 246, 573, 659, 1497, 1537, 1747
BstMAI GTCTC 1 cut(s) 845
BstMBI GATC 6 cut(s) 243, 570, 656, 1494, 1534, 1744
BstMWI GCNNNNNNNGC 9 cut(s) 270, 353, 483, 627, 1603, 1798, 1817, 1840, 1918
BstNSI RCATGY 1 cut(s) 1610
BstPAI GACNNNNGTC 1 cut(s) 1679
BstSFI CTRYAG 1 cut(s) 1944
BstUI CGCG 3 cut(s) 605, 761, 1232
BstV1I GCAGC 9 cut(s) 362, 412, 994, 1069, 1622, 1801, 1879, 1921, 1958
BstXI CCANNNNNNTGG 1 cut(s) 1025
Bsu15I ATCGAT 1 cut(s) 1280
BsuRI GGCC 5 cut(s) 212, 273, 306, 595, 1155
BsuTUI ATCGAT 1 cut(s) 1280
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 5 cut(s) 42, 414, 1240, 1252, 1429
BtsIMutI CAGTG 2 cut(s) 786, 1290
Cac8I GCNNGC 7 cut(s) 210, 398, 578, 623, 1427, 1588, 1608
CfoI GCGC 2 cut(s) 477, 605
Cfr10I RCCGGY 1 cut(s) 1678
Cfr13I GGNCC 2 cut(s) 593, 1442
ClaI ATCGAT 1 cut(s) 1280
CseI GACGC 5 cut(s) 150, 185, 835, 1238, 1684
Csp6I GTAC 4 cut(s) 220, 1145, 1338, 1768
CviAII CATG 6 cut(s) 749, 1001, 1598, 1607, 1765, 1850
CviQI GTAC 4 cut(s) 220, 1145, 1338, 1768
DdeI CTNAG 2 cut(s) 1180, 1293
DpnI GATC 6 cut(s) 245, 572, 658, 1496, 1536, 1746
DpnII GATC 6 cut(s) 243, 570, 656, 1494, 1534, 1744
EaeI YGGCCR 2 cut(s) 210, 271
Eam1104I CTCTTC 3 cut(s) 500, 1587, 1639
EarI CTCTTC 3 cut(s) 500, 1587, 1639
EciI GGCGGA 2 cut(s) 430, 470
Eco130I CCWWGG 2 cut(s) 301, 1150
Eco47I GGWCC 1 cut(s) 1442
Eco57I CTGAAG 1 cut(s) 1304
EcoRI GAATTC 1 cut(s) 1370
EcoT14I CCWWGG 2 cut(s) 301, 1150
ErhI CCWWGG 2 cut(s) 301, 1150
FaeI CATG 6 cut(s) 752, 1004, 1601, 1610, 1768, 1853
FaqI GGGAC 1 cut(s) 532
FatI CATG 6 cut(s) 748, 1000, 1597, 1606, 1764, 1849
FauI CCCGC 5 cut(s) 305, 335, 614, 757, 1323
FbaI TGATCA 1 cut(s) 1534
FblI GTMKAC 2 cut(s) 824, 1673
FokI GGATG 5 cut(s) 29, 401, 1239, 1247, 1416
FspBI CTAG 4 cut(s) 173, 669, 840, 1406
GlaI GCGC 2 cut(s) 476, 604
GsaI CCCAGC 1 cut(s) 885
GsuI CTGGAG 3 cut(s) 455, 637, 965
HaeII RGCGCY 1 cut(s) 478
HaeIII GGCC 5 cut(s) 212, 273, 306, 595, 1155
HapII CCGG 2 cut(s) 596, 1679
HgaI GACGC 5 cut(s) 150, 185, 835, 1238, 1684
HhaI GCGC 2 cut(s) 477, 605
Hin1I GRCGYC 5 cut(s) 142, 177, 504, 827, 1676
Hin1II CATG 6 cut(s) 752, 1004, 1601, 1610, 1768, 1853
Hin6I GCGC 2 cut(s) 475, 603
HinP1I GCGC 2 cut(s) 475, 603
HincII GTYRAC 2 cut(s) 825, 1674
HindII GTYRAC 2 cut(s) 825, 1674
HinfI GANTC 6 cut(s) 821, 1282, 1514, 1670, 1889, 1940
HpaII CCGG 2 cut(s) 596, 1679
HphI GGTGA 2 cut(s) 6, 1054
Hpy166II GTNNAC 3 cut(s) 825, 1069, 1674
Hpy8I GTNNAC 3 cut(s) 825, 1069, 1674
Hpy99I CGWCG 4 cut(s) 144, 506, 829, 1678
HpyAV CCTTC 7 cut(s) 70, 379, 560, 707, 1556, 1897, 1965
HpyCH4III ACNGT 4 cut(s) 313, 790, 1206, 1976
HpyCH4IV ACGT 3 cut(s) 411, 504, 1049
HpyF10VI GCNNNNNNNGC 9 cut(s) 270, 353, 483, 627, 1603, 1798, 1817, 1840, 1918
HpyF3I CTNAG 2 cut(s) 1180, 1293
HpySE526I ACGT 3 cut(s) 411, 504, 1049
Hsp92I GRCGYC 5 cut(s) 142, 177, 504, 827, 1676
Hsp92II CATG 6 cut(s) 752, 1004, 1601, 1610, 1768, 1853
HspAI GCGC 2 cut(s) 475, 603
Ksp22I TGATCA 1 cut(s) 1534
Kzo9I GATC 6 cut(s) 243, 570, 656, 1494, 1534, 1744
LguI GCTCTTC 1 cut(s) 1587
LmnI GCTCC 7 cut(s) 104, 335, 618, 1079, 1249, 1786, 1906
Lsp1109I GCAGC 9 cut(s) 362, 412, 994, 1069, 1622, 1801, 1879, 1921, 1958
LweI GCATC 3 cut(s) 930, 1261, 1438
MaeI CTAG 4 cut(s) 173, 669, 840, 1406
MaeII ACGT 3 cut(s) 411, 504, 1049
MaeIII GTNAC 4 cut(s) 683, 1308, 1617, 1694
MalI GATC 6 cut(s) 245, 572, 658, 1496, 1536, 1746
MbiI CCGCTC 2 cut(s) 330, 621
MboI GATC 6 cut(s) 243, 570, 656, 1494, 1534, 1744
MboII GAAGA 9 cut(s) 55, 364, 487, 619, 988, 1382, 1604, 1656, 1850
MluCI AATT 9 cut(s) 185, 275, 1099, 1370, 1396, 1500, 1538, 1622, 1711
MlyI GAGTC 2 cut(s) 830, 1679
MmeI TCCRAC 6 cut(s) 126, 357, 798, 840, 915, 933
MroXI GAANNNNTTC 1 cut(s) 1370
MseI TTAA 3 cut(s) 834, 1823, 1971
MspI CCGG 2 cut(s) 596, 1679
Mva1269I GAATGC 3 cut(s) 934, 1036, 1885
MvnI CGCG 3 cut(s) 605, 761, 1232
MwoI GCNNNNNNNGC 9 cut(s) 270, 353, 483, 627, 1603, 1798, 1817, 1840, 1918
NdeII GATC 6 cut(s) 243, 570, 656, 1494, 1534, 1744
NlaIII CATG 6 cut(s) 752, 1004, 1601, 1610, 1768, 1853
NlaIV GGNNCC 1 cut(s) 1016
NmeAIII GCCGAG 3 cut(s) 339, 469, 769
NmuCI GTSAC 3 cut(s) 683, 1308, 1617
NspI RCATGY 1 cut(s) 1610
PaeI GCATGC 1 cut(s) 1610
PciSI GCTCTTC 1 cut(s) 1587
PcsI WCGNNNNNNNCGW 3 cut(s) 148, 393, 510
PctI GAATGC 3 cut(s) 934, 1036, 1885
PdmI GAANNNNTTC 1 cut(s) 1370
PfeI GAWTC 4 cut(s) 1282, 1514, 1889, 1940
PflMI CCANNNNNTGG 1 cut(s) 1451
PleI GAGTC 2 cut(s) 829, 1678
PpsI GAGTC 2 cut(s) 829, 1678
PshAI GACNNNNGTC 1 cut(s) 1679
PshBI ATTAAT 1 cut(s) 834
PspFI CCCAGC 1 cut(s) 881
PspN4I GGNNCC 1 cut(s) 1016
PspPI GGNCC 2 cut(s) 593, 1442
PstI CTGCAG 1 cut(s) 1948
RsaI GTAC 4 cut(s) 221, 1146, 1339, 1769
RsaNI GTAC 4 cut(s) 220, 1145, 1338, 1768
SalI GTCGAC 2 cut(s) 823, 1672
SapI GCTCTTC 1 cut(s) 1587
SaqAI TTAA 3 cut(s) 834, 1823, 1971
Sau3AI GATC 6 cut(s) 243, 570, 656, 1494, 1534, 1744
Sau96I GGNCC 2 cut(s) 593, 1442
ScaI AGTACT 1 cut(s) 1339
SchI GAGTC 2 cut(s) 830, 1679
SfaNI GCATC 3 cut(s) 930, 1261, 1438
SfcI CTRYAG 1 cut(s) 1944
SinI GGWCC 1 cut(s) 1442
SmlI CTYRAG 4 cut(s) 239, 694, 803, 1893
SmoI CTYRAG 4 cut(s) 239, 694, 803, 1893
SphI GCATGC 1 cut(s) 1610
Sse9I AATT 9 cut(s) 185, 275, 1099, 1370, 1396, 1500, 1538, 1622, 1711
SspMI CTAG 4 cut(s) 173, 669, 840, 1406
StyI CCWWGG 2 cut(s) 301, 1150
TaaI ACNGT 4 cut(s) 313, 790, 1206, 1976
TaiI ACGT 3 cut(s) 414, 507, 1052
TasI AATT 9 cut(s) 185, 275, 1099, 1370, 1396, 1500, 1538, 1622, 1711
TatI WGTACW 2 cut(s) 1337, 1767
TauI GCSGC 6 cut(s) 182, 215, 350, 356, 486, 764
TfiI GAWTC 4 cut(s) 1282, 1514, 1889, 1940
Tru1I TTAA 3 cut(s) 834, 1823, 1971
Tru9I TTAA 3 cut(s) 834, 1823, 1971
TscAI CASTG 2 cut(s) 793, 1297
TseFI GTSAC 3 cut(s) 683, 1308, 1617
TseI GCWGC 9 cut(s) 350, 400, 982, 1082, 1610, 1789, 1867, 1909, 1946
Tsp45I GTSAC 3 cut(s) 683, 1308, 1617
TspDTI ATGAA 4 cut(s) 1615, 1753, 1916, 1953
TspGWI ACGGA 2 cut(s) 502, 1351
TspRI CASTG 2 cut(s) 793, 1297
Van91I CCANNNNNTGG 1 cut(s) 1451
VpaK11BI GGWCC 1 cut(s) 1442
VspI ATTAAT 1 cut(s) 834
XapI RAATTY 3 cut(s) 1370, 1396, 1711
XbaI TCTAGA 2 cut(s) 668, 1405
XceI RCATGY 1 cut(s) 1610
XmiI GTMKAC 2 cut(s) 824, 1673
XmnI GAANNNNTTC 1 cut(s) 1370
XspI CTAG 4 cut(s) 173, 669, 840, 1406
ZraI GACGTC 1 cut(s) 505
ZrmI AGTACT 1 cut(s) 1339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.