Rh2CG318000

Arogenate dehydrogenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003175
Physical Location & Seq
Reverse (-)
40719977 .. 40723131
3155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG318000.1

Sequence Viewer

Length: 1806 bp
ATGCTGAAGATTGCCATCATCGGCTTTGGCAACTTGGGACAATCCCTTGCCAAGACCTTTGCTGACCTAGGCCACATGGTCCTTGCACATTCTAGATGTGACCACTCCAAAATGGCCCAAGACCTCCGCATTTCATATTTCTCTGACCCGCAAGACCTCTGTGAACAGAATCCACAGGTCATTTTGCTATGCACCTCCATAATCTCCACAGAATCCATCCTCAAATCTTTACCTCTCCAACGCCTTAGGCATGACACATTGTTTGTTGACATGCTTCCTGTCAAAGAGTTTTCAAAGGCATTGTTACTCAAGTTGTTGCCTAGCTATTTTGATGTCCTTTGTACCAACCCAATGTTCGGAGTTGAGAGAGGGAAACAAGAATGGAATGGCATGTTTTTTGTGTATGAAAAGGTTCGAATTGTCTCTGACGGAGTGGGGATTTCTCTCTGTGATAACTTCTTAAACATATTTGAGAAACAAGGGTGTAGGATGGTGGAAGTAAGCTGTTCAGAGCAGGATAAGTATGCCGCAGGTTCACAGTTTATTACCCATGCAGTTGGGAGAGTCTTGGGGATGTTGCCATTGGAATCGAGTCCAATAAATACAAAAGGTTATGAAACATTGTTGGATTTGGTGGACAACACAGCTAGGGATAGTTTTGATTTATATTATGAGTTGTTCATGTACAACAAAAATGCATTTGAGATGTTTGAGAGGTTGGACTTGGCTGTTGACACTTTGAAGAAACAGCTTTTTGGTTATTTGCATGATGTTGTGAGAAAGCAAGTATTTGATAATGCAGAGAAGGTAAAAACGGGGCACGAGGAGTACAAGGTGTCTAACCCATCTCACAATGGAGCTGCCTCCGAATCTTCTTCAAAAGCTATGAGGTCACATAAGATTGCTCAGGTATCTAACCGTAAAGCACAGATAATATCTGACCACTTTGATGACAACTCAAGGCTCAAGATTGCAATTGTTGGTTTTGGGAACTTCGGTCAGTTTCTTGCTAAAACTATTATACGTCAAGGTCATATAGTTCTAGCCTATTCTCGATCAGACTACTATGATGTGGCTCAAAAGTTGGGGGTAACTTACTTCTCTGATGTCGAGGAACTTTGCAAAGAGCATCCAGAAGTGATACTTTTTTGTACCTCTATTCTTTCAACTGAGAAAGTTCTCAGGTCATTTCCAGTTCAGAGGTTGATGAGGAATACTTTGTTTGTTGATGTTCTTTCAGTTAAAGAATTTCCAAAAAACTTGTTTCTTCAAAAGTTGCCATTGAATTTTGATATTCTCTGTACTCATCCTATGTTTGGACCAGATAGTGGTAAAAATGGGTGGGAGGCTCTTCCTTTTGTTTATGATAAGGTCAGGGTGGGAAGTGATGAATCAAGGGTATCACTCTGTGACCGATTTCTTGATATCTTTGCCCTTGAAGGGTGTCGAATGGTTGAAATGTCTTGTGCAGAGCATGATAGGCATGCAGCAGGGTCACAATTCCTTACGCACACAATAGGAAGGGTTTTGGAGAAGCTGGGTTTGGAGTCGACACCTATCTTTACAAATGGTTACAAGACTTTATTGAATTTGGTGGAGACTACAGTAGGAGATAGTTTTGATCTTTACTATGGCTTGTTCATGTATAATGTAAATGCAATGGACCAGCTAAATAGATTGGGCATGGCTTTTGACTCATTAGAGGAGCAGTTTTTGGGGCGTTTGCATGGTGTTCTCCATAAGCAACATTCTGAGAATGCAACCAAAACCTTGTTGCCAAATCATCCAAGAATGCAGCTCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00400 ko01100 ko01110 ko01230 map00400 map01100 map01110 map01230
Pfam Domains
Protein Families
F420_oxidored PDH

Protein Analysis

601

Amino Acids

68.22

Weight (kDa)

6.24

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F420_oxidored PF03807 3 - 92 8.1e-09 NADP oxidoreductase coenzyme F420-dependent
2-Hacid_dh_C PF02826 3 - 90 4.9e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
TYRAAT1_C PF26213 189 - 262 2.1e-31 TYRAAT1-like, C-terminal domain
F420_oxidored PF03807 323 - 412 1.3e-08 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 337 - 445 2.9e-07 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 509 - 582 8.6e-31 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 521
AccB7I CCANNNNNTGG 1 cut(s) 1328
AccI GTMKAC 1 cut(s) 1550
AciI CCGC 3 cut(s) 127, 149, 528
AcsI RAATTY 3 cut(s) 1247, 1285, 1588
AcuI CTGAAG 1 cut(s) 26
AdeI CACNNNGTG 1 cut(s) 1409
AfaI GTAC 5 cut(s) 343, 686, 830, 1153, 1303
AfiI CCNNNNNNNGG 4 cut(s) 356, 1316, 1328, 1440
AgsI TTSAA 9 cut(s) 294, 742, 879, 1167, 1271, 1285, 1439, 1457, 1588
AjuI GAANNNNNNNTTGG 4 cut(s) 338, 370, 1526, 1558
AluBI AGCT 9 cut(s) 324, 504, 647, 751, 860, 884, 1537, 1669, 1798
AluI AGCT 9 cut(s) 324, 504, 647, 751, 860, 884, 1537, 1669, 1798
Alw26I GTCTC 2 cut(s) 427, 1592
AoxI GGCC 2 cut(s) 70, 114
ApeKI GCWGC 3 cut(s) 860, 1487, 1795
ApoI RAATTY 3 cut(s) 1247, 1285, 1588
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
Asp700I GAANNNNTTC 1 cut(s) 411
AspA2I CCTAGG 1 cut(s) 67
AspS9I GGNCC 4 cut(s) 79, 115, 1319, 1663
AsuII TTCGAA 1 cut(s) 415
AvaII GGWCC 3 cut(s) 79, 1319, 1663
AvrII CCTAGG 1 cut(s) 67
AxyI CCTNAGG 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 822
BauI CACGAG 1 cut(s) 821
BbvI GCAGC 2 cut(s) 847, 1499
BccI CCATC 4 cut(s) 23, 224, 484, 853
BcoDI GTCTC 2 cut(s) 427, 1592
BfaI CTAG 5 cut(s) 68, 93, 321, 648, 1043
BfmI CTRYAG 1 cut(s) 1602
BfuAI ACCTGC 1 cut(s) 521
BisI GCNGC 4 cut(s) 528, 861, 1488, 1796
BlnI CCTAGG 1 cut(s) 67
BlsI GCNGC 4 cut(s) 529, 862, 1489, 1797
Bme18I GGWCC 3 cut(s) 79, 1319, 1663
BmgT120I GGNCC 4 cut(s) 79, 115, 1319, 1663
BmsI GCATC 1 cut(s) 1138
Bpu10I CCTNAGC 1 cut(s) 906
Bpu14I TTCGAA 1 cut(s) 415
BpuEI CTTGAG 3 cut(s) 293, 943, 950
BsaBI GATNNNNATC 1 cut(s) 14
BsaJI CCNNGG 1 cut(s) 67
Bsc4I CCNNNNNNNGG 4 cut(s) 356, 1316, 1328, 1440
Bse1I ACTGG 1 cut(s) 1193
Bse21I CCTNAGG 1 cut(s) 245
Bse3DI GCAATG 1 cut(s) 1665
Bse8I GATNNNNATC 1 cut(s) 14
BseDI CCNNGG 1 cut(s) 67
BseGI GGATG 6 cut(s) 216, 495, 579, 1129, 1306, 1783
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 4 cut(s) 356, 1316, 1328, 1440
BseMI GCAATG 1 cut(s) 1665
BseMII CTCAG 4 cut(s) 920, 1161, 1195, 1743
BseNI ACTGG 1 cut(s) 1193
BseRI GAGGAG 2 cut(s) 839, 1718
BseSI GKGCMC 1 cut(s) 822
BseXI GCAGC 2 cut(s) 847, 1499
BseYI CCCAGC 1 cut(s) 1537
BsgI GTGCAG 1 cut(s) 1488
BshFI GGCC 2 cut(s) 72, 116
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 4 cut(s) 356, 1316, 1328, 1440
BsmAI GTCTC 2 cut(s) 427, 1592
BsmFI GGGAC 1 cut(s) 51
BsmI GAATGC 2 cut(s) 1762, 1797
BsnI GGCC 2 cut(s) 72, 116
Bsp119I TTCGAA 1 cut(s) 415
Bsp1286I GDGCHC 1 cut(s) 822
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 2 cut(s) 1055, 1621
BspACI CCGC 3 cut(s) 127, 149, 528
BspANI GGCC 2 cut(s) 72, 116
BspCNI CTCAG 4 cut(s) 919, 1162, 1194, 1744
BspMI ACCTGC 1 cut(s) 521
BspQI GCTCTTC 1 cut(s) 1356
BspT104I TTCGAA 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 1665
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 1 cut(s) 1193
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 2 cut(s) 1055, 1621
BssSI CACGAG 1 cut(s) 821
BssT1I CCWWGG 1 cut(s) 67
Bst2BI CACGAG 1 cut(s) 821
Bst4CI ACNGT 3 cut(s) 540, 920, 1606
Bst6I CTCTTC 1 cut(s) 1356
BstAUI TGTACA 1 cut(s) 684
BstBI TTCGAA 1 cut(s) 415
BstC8I GCNNGC 1 cut(s) 1485
BstDEI CTNAG 5 cut(s) 245, 906, 1170, 1181, 1752
BstF5I GGATG 6 cut(s) 216, 495, 579, 1129, 1306, 1783
BstKTI GATC 2 cut(s) 1058, 1624
BstMAI GTCTC 2 cut(s) 427, 1592
BstMBI GATC 2 cut(s) 1055, 1621
BstMWI GCNNNNNNNGC 1 cut(s) 1480
BstNSI RCATGY 3 cut(s) 274, 394, 1487
BstSFI CTRYAG 1 cut(s) 1602
BstSLI GKGCMC 1 cut(s) 822
BstV1I GCAGC 2 cut(s) 847, 1499
BstXI CCANNNNNNTGG 1 cut(s) 557
Bsu36I CCTNAGG 1 cut(s) 245
BsuRI GGCC 2 cut(s) 72, 116
BtsCI GGATG 6 cut(s) 216, 495, 579, 1129, 1306, 1783
BveI ACCTGC 1 cut(s) 521
Cac8I GCNNGC 1 cut(s) 1485
Cfr13I GGNCC 4 cut(s) 79, 115, 1319, 1663
Csp6I GTAC 5 cut(s) 342, 685, 829, 1152, 1302
CviQI GTAC 5 cut(s) 342, 685, 829, 1152, 1302
DdeI CTNAG 5 cut(s) 245, 906, 1170, 1181, 1752
DpnI GATC 2 cut(s) 1057, 1623
DpnII GATC 2 cut(s) 1055, 1621
DraIII CACNNNGTG 1 cut(s) 1409
Eam1104I CTCTTC 1 cut(s) 1356
EarI CTCTTC 1 cut(s) 1356
Eco130I CCWWGG 1 cut(s) 67
Eco32I GATATC 1 cut(s) 1426
Eco47I GGWCC 3 cut(s) 79, 1319, 1663
Eco57I CTGAAG 1 cut(s) 26
Eco81I CCTNAGG 1 cut(s) 245
EcoRV GATATC 1 cut(s) 1426
EcoT14I CCWWGG 1 cut(s) 67
EcoT22I ATGCAT 1 cut(s) 700
ErhI CCWWGG 1 cut(s) 67
FalI AAGNNNNNCTT 2 cut(s) 1128, 1160
FaqI GGGAC 1 cut(s) 51
FauI CCCGC 1 cut(s) 156
FblI GTMKAC 1 cut(s) 1550
Fnu4HI GCNGC 4 cut(s) 528, 861, 1488, 1796
FokI GGATG 6 cut(s) 203, 502, 586, 1116, 1293, 1770
Fsp4HI GCNGC 4 cut(s) 528, 861, 1488, 1796
FspBI CTAG 5 cut(s) 68, 93, 321, 648, 1043
GluI GCNGC 4 cut(s) 528, 861, 1488, 1796
GsaI CCCAGC 1 cut(s) 1541
HaeIII GGCC 2 cut(s) 72, 116
HincII GTYRAC 3 cut(s) 268, 733, 1551
HindII GTYRAC 3 cut(s) 268, 733, 1551
HinfI GANTC 9 cut(s) 169, 212, 564, 587, 592, 869, 1391, 1547, 1694
Hpy166II GTNNAC 6 cut(s) 164, 268, 536, 637, 733, 1551
Hpy188III TCNNGA 5 cut(s) 93, 967, 1053, 1133, 1421
Hpy8I GTNNAC 6 cut(s) 164, 268, 536, 637, 733, 1551
HpyAV CCTTC 3 cut(s) 799, 1433, 1515
HpyCH4III ACNGT 3 cut(s) 540, 920, 1606
HpyCH4IV ACGT 1 cut(s) 1024
HpyF10VI GCNNNNNNNGC 1 cut(s) 1480
HpyF3I CTNAG 5 cut(s) 245, 906, 1170, 1181, 1752
HpySE526I ACGT 1 cut(s) 1024
Kzo9I GATC 2 cut(s) 1055, 1621
LguI GCTCTTC 1 cut(s) 1356
LmnI GCTCC 3 cut(s) 857, 1705, 1803
Lsp1109I GCAGC 2 cut(s) 847, 1499
LweI GCATC 1 cut(s) 1138
MaeI CTAG 5 cut(s) 68, 93, 321, 648, 1043
MaeII ACGT 1 cut(s) 1024
MaeIII GTNAC 7 cut(s) 98, 303, 891, 1090, 1409, 1494, 1571
MalI GATC 2 cut(s) 1057, 1623
MboI GATC 2 cut(s) 1055, 1621
MboII GAAGA 6 cut(s) 19, 754, 864, 867, 1259, 1343
MfeI CAATTG 1 cut(s) 975
MhlI GDGCHC 1 cut(s) 822
MluCI AATT 6 cut(s) 417, 975, 1247, 1285, 1499, 1588
MlyI GAGTC 4 cut(s) 573, 601, 1556, 1688
MmeI TCCRAC 3 cut(s) 262, 606, 699
Mph1103I ATGCAT 1 cut(s) 700
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 2 cut(s) 461, 1242
MslI CAYNNNNRTG 1 cut(s) 948
MunI CAATTG 1 cut(s) 975
Mva1269I GAATGC 2 cut(s) 1762, 1797
MwoI GCNNNNNNNGC 1 cut(s) 1480
NdeII GATC 2 cut(s) 1055, 1621
NmuCI GTSAC 4 cut(s) 98, 891, 1409, 1494
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 3 cut(s) 274, 394, 1487
NspV TTCGAA 1 cut(s) 415
PaeI GCATGC 1 cut(s) 1487
PciSI GCTCTTC 1 cut(s) 1356
PctI GAATGC 2 cut(s) 1762, 1797
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 5 cut(s) 169, 212, 587, 869, 1391
PflMI CCANNNNNTGG 1 cut(s) 1328
PkrI GCNGC 4 cut(s) 529, 862, 1489, 1797
PleI GAGTC 4 cut(s) 572, 600, 1555, 1688
PpsI GAGTC 4 cut(s) 572, 600, 1555, 1688
PspFI CCCAGC 1 cut(s) 1537
PspPI GGNCC 4 cut(s) 79, 115, 1319, 1663
RsaI GTAC 5 cut(s) 343, 686, 830, 1153, 1303
RsaNI GTAC 5 cut(s) 342, 685, 829, 1152, 1302
RseI CAYNNNNRTG 1 cut(s) 948
SalI GTCGAC 1 cut(s) 1549
SapI GCTCTTC 1 cut(s) 1356
SaqAI TTAA 2 cut(s) 461, 1242
SatI GCNGC 4 cut(s) 528, 861, 1488, 1796
Sau3AI GATC 2 cut(s) 1055, 1621
Sau96I GGNCC 4 cut(s) 79, 115, 1319, 1663
SchI GAGTC 4 cut(s) 573, 601, 1556, 1688
SduI GDGCHC 1 cut(s) 822
SfaNI GCATC 1 cut(s) 1138
SfcI CTRYAG 1 cut(s) 1602
SfuI TTCGAA 1 cut(s) 415
SinI GGWCC 3 cut(s) 79, 1319, 1663
SmiMI CAYNNNNRTG 1 cut(s) 948
SmlI CTYRAG 3 cut(s) 308, 958, 965
SmoI CTYRAG 3 cut(s) 308, 958, 965
SphI GCATGC 1 cut(s) 1487
Sse9I AATT 6 cut(s) 417, 975, 1247, 1285, 1499, 1588
SsiI CCGC 3 cut(s) 127, 149, 528
SspMI CTAG 5 cut(s) 68, 93, 321, 648, 1043
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 3 cut(s) 540, 920, 1606
TaiI ACGT 1 cut(s) 1027
TaqI TCGA 6 cut(s) 415, 590, 1054, 1110, 1447, 1550
TaqII GACCGA 2 cut(s) 986, 1428
TasI AATT 6 cut(s) 417, 975, 1247, 1285, 1499, 1588
TatI WGTACW 3 cut(s) 684, 828, 1301
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 5 cut(s) 169, 212, 587, 869, 1391
Tru1I TTAA 2 cut(s) 461, 1242
Tru9I TTAA 2 cut(s) 461, 1242
TseFI GTSAC 4 cut(s) 98, 891, 1409, 1494
TseI GCWGC 3 cut(s) 860, 1487, 1795
Tsp45I GTSAC 4 cut(s) 98, 891, 1409, 1494
TspDTI ATGAA 6 cut(s) 123, 420, 630, 670, 1404, 1630
TspGWI ACGGA 1 cut(s) 444
Van91I CCANNNNNTGG 1 cut(s) 1328
VpaK11BI GGWCC 3 cut(s) 79, 1319, 1663
XapI RAATTY 3 cut(s) 1247, 1285, 1588
XbaI TCTAGA 1 cut(s) 92
XceI RCATGY 3 cut(s) 274, 394, 1487
XmaJI CCTAGG 1 cut(s) 67
XmiI GTMKAC 1 cut(s) 1550
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 5 cut(s) 68, 93, 321, 648, 1043
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.