FvH4_6g26860

Arogenate dehydrogenase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003175
Physical Location & Seq
Reverse (-)
20538203 .. 20542242
4040 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26860.t2

Sequence Viewer

Length: 1776 bp
ATGCTCAAGATTGCCATCATCGGCTTTGGCAACTTGGGTCAATCCCTTGCCAAGACCTTTGCCCACCTAGGACACATTGTTCTTGCACATTCTAGATGTGAACACTCCAAAATGGCCCAAGACCTCGGCATTTCATATTTTTCTAACCCGCAAGAACTCTGTGAACAGAATCCACAGGTCATTTTGCTATGCACCTCCATAATATCCACAGAATCCGTCCTCAAATCTTTACCTCTTCAGAGCCTTACACATGACACATTGTTTGTTGACATGCTTCCTGTCAAGGAGTTTTCAAAAGCTTTGTTACTGAAGATGTTGCCTAGCAATCTAGCTGTTGTTTGCACCAACCCGATGTTCGGAGTTGAAAGAGGCAAACATGAGTGGAATGGCAGTTTTTTGGTGTACGAAAAGGTTCGAATTGGCTCTGACAGTGTGGGGATTTCGTTGTGTGATAACTTCTTAAACATCTTTGAGAAAGAAGGATGTAGAATGGTGGAAGTAAGCTGTTCTGTTCAAGATAAGTATGTAGCAGGCTCGCAGTTTATTACCCACACAGTTGGGAGAGTCTTGGAAAAGTTAACATTGGAATCGACGCCAATAAATACAAAAGGTTATGAGACATTGTTGGATTTGGTGGACAACACAGCTGGGGATAGCTTTGATTTATATTGTGAGTTGTTCATATACAATAAAAATGCATTTGAGATGTTTGACAGGTTGGACTTGGCTTTTAACACTTTGAAGAAGCAACTTTTTGGTCATTTGCATGATATTGTGAGAAAGCAACTATTTGATAGTGCAGAGAAGGTACATGCGGAGTACATGGTGTGTAATCCATCCCACAATGGAGCTGCCTTGAGGTCGCATTCTCAGGTACTTAACCAAAAAGCACATATATCTGAACACTATGATGACAAGTCGATGCTCAAGATTGCAATTGTTGGTTTTGGGAACTTCGGTCAGTTCCTTGCTAAAACTATTATACGTCAAGGTCATAAAGTTCTAGCCTATTCTCGATCAGACTACTATGATGTGGCTCAAGAGATGGGGGTAATTTACTTATCCGACGTTGAGGCTCTTTGCGAAGAGCATCCAGAAGTGATGCTCTTTTGTACCTCTATCCTTTCAACCGAGAAAGTTCTCAGATCATTTCCAGTTCAGAGGTTGATGAGGAATACATTGTTTGTTGATGTGCTTTCAGTTAAAGAATTTCCAAGAAACTTGTTTCTTCAAAAGTTGCCGTTGGATTTTGATATTCTCTGTACGCATCCTATGTTTGGACCAGAGAGTGGTAAAAATGGGTGGAATGCTCTTCCTTTTGTTTATGATAAGGTCAGGGTGGGAAGTGATAAATCAAGGGTATCACTCTGTGACCGATTCCTTGATATCTTTGCTCTAGAAGGGTGCCGAATGGTTGAAATGTCTTGTTCAGAGCATGATAGGCATGCAGCAGGGTCACAATTCATTACGCACACCATAGGAAGGGTTTTGGAAAAGCTGGGTTTGGAGTCAACACCTATCCTTCCAAATGGTTACAAGGCTTTATTGAATTTGGTGGAGAATACAGTAGGAGATAGTTTTGATCTTTACCATGGCTTGTTCATGTATAATGTAAATGCAATAGACCAGCTGAATAGATTGGACATGGCTTTTAACTCGTTAAAGGAACAGTTTTTGAGGCGTTTGCATGGTGTTGTCCATAGCCAACATTTCGAGAATGCAAGCAGAACCTTGTTGCCAAATCATCCAGAGAATGCAGCTCCACTGAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00400 ko01100 ko01110 ko01230 map00400 map01100 map01110 map01230
Pfam Domains
Protein Families
F420_oxidored PDH

Protein Analysis

592

Amino Acids

66.76

Weight (kDa)

6.27

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F420_oxidored PF03807 3 - 92 5e-10 NADP oxidoreductase coenzyme F420-dependent
2-Hacid_dh_C PF02826 3 - 90 6.5e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
PDH_N PF02153 26 - 122 5.3e-06 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 189 - 262 1.2e-31 TYRAAT1-like, C-terminal domain
F420_oxidored PF03807 310 - 399 5.7e-07 NADP oxidoreductase coenzyme F420-dependent
PDH_N PF02153 324 - 431 3.5e-06 Prephenate dehydrogenase, nucleotide-binding domain
TYRAAT1_C PF26213 496 - 569 6e-28 TYRAAT1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1404
AccB7I CCANNNNNTGG 1 cut(s) 1289
AciI CCGC 2 cut(s) 149, 815
AcsI RAATTY 2 cut(s) 1208, 1549
AcuI CTGAAG 2 cut(s) 221, 329
AcyI GRCGYC 1 cut(s) 593
AdeI CACNNNGTG 1 cut(s) 1370
AfaI GTAC 6 cut(s) 404, 810, 821, 876, 1114, 1264
AfiI CCNNNNNNNGG 4 cut(s) 356, 1277, 1289, 1482
AgsI TTSAA 8 cut(s) 294, 365, 515, 742, 1128, 1232, 1418, 1549
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AloI GAACNNNNNNTCC 2 cut(s) 63, 95
AluBI AGCT 9 cut(s) 299, 332, 504, 647, 657, 851, 1498, 1630, 1760
AluI AGCT 9 cut(s) 299, 332, 504, 647, 657, 851, 1498, 1630, 1760
Alw26I GTCTC 1 cut(s) 611
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 3 cut(s) 851, 1448, 1757
ApoI RAATTY 2 cut(s) 1208, 1549
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
Asp700I GAANNNNTTC 1 cut(s) 411
AspA2I CCTAGG 1 cut(s) 67
AspS9I GGNCC 2 cut(s) 115, 1280
AsuII TTCGAA 1 cut(s) 415
AvaII GGWCC 1 cut(s) 1280
AvrII CCTAGG 1 cut(s) 67
BanI GGYRCC 1 cut(s) 1404
BbvI GCAGC 3 cut(s) 838, 1460, 1769
BccI CCATC 3 cut(s) 23, 844, 1039
BceAI ACGGC 1 cut(s) 1225
BcoDI GTCTC 1 cut(s) 611
BfaI CTAG 6 cut(s) 68, 93, 321, 329, 1004, 1397
BisI GCNGC 3 cut(s) 852, 1449, 1758
BlnI CCTAGG 1 cut(s) 67
BlsI GCNGC 3 cut(s) 853, 1450, 1759
Bme18I GGWCC 1 cut(s) 1280
BmgT120I GGNCC 2 cut(s) 115, 1280
BmiI GGNNCC 1 cut(s) 1406
BmsI GCATC 4 cut(s) 912, 1092, 1099, 1276
Bpu14I TTCGAA 1 cut(s) 415
BpuEI CTTGAG 3 cut(s) 877, 911, 1023
BsaBI GATNNNNATC 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 593
BsaJI CCNNGG 3 cut(s) 67, 124, 1591
Bsc4I CCNNNNNNNGG 4 cut(s) 356, 1277, 1289, 1482
Bse1I ACTGG 1 cut(s) 1154
Bse8I GATNNNNATC 1 cut(s) 14
BseDI CCNNGG 3 cut(s) 67, 124, 1591
BseGI GGATG 5 cut(s) 488, 836, 1090, 1267, 1744
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 4 cut(s) 356, 1277, 1289, 1482
BseMII CTCAG 3 cut(s) 884, 1156, 1757
BseNI ACTGG 1 cut(s) 1154
BseXI GCAGC 3 cut(s) 838, 1460, 1769
BseYI CCCAGC 2 cut(s) 647, 1498
BsgI GTGCAG 1 cut(s) 819
BshFI GGCC 1 cut(s) 116
BshNI GGYRCC 1 cut(s) 1404
BslI CCNNNNNNNGG 4 cut(s) 356, 1277, 1289, 1482
BsmAI GTCTC 1 cut(s) 611
BsmI GAATGC 4 cut(s) 865, 1312, 1723, 1759
BsnI GGCC 1 cut(s) 116
Bsp119I TTCGAA 1 cut(s) 415
Bsp143I GATC 3 cut(s) 1016, 1145, 1582
Bsp19I CCATGG 1 cut(s) 1591
BspACI CCGC 2 cut(s) 149, 815
BspANI GGCC 1 cut(s) 116
BspCNI CTCAG 3 cut(s) 883, 1155, 1758
BspLI GGNNCC 1 cut(s) 1406
BspQI GCTCTTC 2 cut(s) 1080, 1317
BspT104I TTCGAA 1 cut(s) 415
BspT107I GGYRCC 1 cut(s) 1404
BsrI ACTGG 1 cut(s) 1154
BssECI CCNNGG 3 cut(s) 67, 124, 1591
BssMI GATC 3 cut(s) 1016, 1145, 1582
BssNI GRCGYC 1 cut(s) 593
BssT1I CCWWGG 2 cut(s) 67, 1591
Bst4CI ACNGT 4 cut(s) 431, 556, 1567, 1671
Bst6I CTCTTC 3 cut(s) 240, 1080, 1317
BstACI GRCGYC 1 cut(s) 593
BstBI TTCGAA 1 cut(s) 415
BstC8I GCNNGC 4 cut(s) 532, 536, 1446, 1723
BstDEI CTNAG 3 cut(s) 870, 1142, 1766
BstDSI CCRYGG 1 cut(s) 1591
BstF5I GGATG 5 cut(s) 488, 836, 1090, 1267, 1744
BstKTI GATC 3 cut(s) 1019, 1148, 1585
BstMAI GTCTC 1 cut(s) 611
BstMBI GATC 3 cut(s) 1016, 1145, 1582
BstMWI GCNNNNNNNGC 1 cut(s) 1441
BstNSI RCATGY 3 cut(s) 274, 815, 1448
BstV1I GCAGC 3 cut(s) 838, 1460, 1769
BstXI CCANNNNNNTGG 1 cut(s) 557
BsuRI GGCC 1 cut(s) 116
BtgI CCRYGG 1 cut(s) 1591
BtsCI GGATG 5 cut(s) 488, 836, 1090, 1267, 1744
BtsIMutI CAGTG 2 cut(s) 436, 1763
Cac8I GCNNGC 4 cut(s) 532, 536, 1446, 1723
Cfr13I GGNCC 2 cut(s) 115, 1280
CseI GACGC 1 cut(s) 601
Csp6I GTAC 6 cut(s) 403, 809, 820, 875, 1113, 1263
CviQI GTAC 6 cut(s) 403, 809, 820, 875, 1113, 1263
DdeI CTNAG 3 cut(s) 870, 1142, 1766
DpnI GATC 3 cut(s) 1018, 1147, 1584
DpnII GATC 3 cut(s) 1016, 1145, 1582
DraIII CACNNNGTG 1 cut(s) 1370
Eam1104I CTCTTC 3 cut(s) 240, 1080, 1317
EarI CTCTTC 3 cut(s) 240, 1080, 1317
Eco130I CCWWGG 2 cut(s) 67, 1591
Eco32I GATATC 1 cut(s) 1387
Eco47I GGWCC 1 cut(s) 1280
Eco57I CTGAAG 2 cut(s) 221, 329
EcoRV GATATC 1 cut(s) 1387
EcoT14I CCWWGG 2 cut(s) 67, 1591
EcoT22I ATGCAT 1 cut(s) 700
ErhI CCWWGG 2 cut(s) 67, 1591
FauI CCCGC 1 cut(s) 156
Fnu4HI GCNGC 3 cut(s) 852, 1449, 1758
FokI GGATG 5 cut(s) 495, 823, 1077, 1254, 1731
Fsp4HI GCNGC 3 cut(s) 852, 1449, 1758
FspBI CTAG 6 cut(s) 68, 93, 321, 329, 1004, 1397
GluI GCNGC 3 cut(s) 852, 1449, 1758
GsaI CCCAGC 2 cut(s) 651, 1502
HaeIII GGCC 1 cut(s) 116
HgaI GACGC 1 cut(s) 601
Hin1I GRCGYC 1 cut(s) 593
HincII GTYRAC 3 cut(s) 268, 579, 1512
HindII GTYRAC 3 cut(s) 268, 579, 1512
HindIII AAGCTT 1 cut(s) 297
HinfI GANTC 6 cut(s) 169, 212, 564, 587, 1377, 1508
HpaI GTTAAC 1 cut(s) 579
Hpy166II GTNNAC 7 cut(s) 101, 164, 268, 403, 579, 637, 1512
Hpy188I TCNGA 9 cut(s) 240, 359, 427, 901, 1021, 1066, 1145, 1161, 1432
Hpy8I GTNNAC 7 cut(s) 101, 164, 268, 403, 579, 637, 1512
Hpy99I CGWCG 2 cut(s) 595, 1070
HpyAV CCTTC 5 cut(s) 473, 799, 1394, 1476, 1532
HpyCH4III ACNGT 4 cut(s) 431, 556, 1567, 1671
HpyCH4IV ACGT 2 cut(s) 985, 1068
HpyF10VI GCNNNNNNNGC 1 cut(s) 1441
HpyF3I CTNAG 3 cut(s) 870, 1142, 1766
HpySE526I ACGT 2 cut(s) 985, 1068
Hsp92I GRCGYC 1 cut(s) 593
KspAI GTTAAC 1 cut(s) 579
Kzo9I GATC 3 cut(s) 1016, 1145, 1582
LguI GCTCTTC 2 cut(s) 1080, 1317
LmnI GCTCC 2 cut(s) 848, 1765
Lsp1109I GCAGC 3 cut(s) 838, 1460, 1769
LweI GCATC 4 cut(s) 912, 1092, 1099, 1276
MaeI CTAG 6 cut(s) 68, 93, 321, 329, 1004, 1397
MaeII ACGT 2 cut(s) 985, 1068
MaeIII GTNAC 4 cut(s) 303, 1370, 1455, 1532
MalI GATC 3 cut(s) 1018, 1147, 1584
MboI GATC 3 cut(s) 1016, 1145, 1582
MboII GAAGA 6 cut(s) 227, 322, 754, 1097, 1220, 1304
MfeI CAATTG 1 cut(s) 936
MluCI AATT 6 cut(s) 417, 936, 1053, 1208, 1460, 1549
MlyI GAGTC 2 cut(s) 573, 1517
MmeI TCCRAC 4 cut(s) 606, 699, 1089, 1224
Mph1103I ATGCAT 1 cut(s) 700
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 7 cut(s) 461, 578, 732, 879, 1203, 1653, 1661
MslI CAYNNNNRTG 2 cut(s) 765, 909
MspA1I CMGCKG 2 cut(s) 647, 1630
MunI CAATTG 1 cut(s) 936
Mva1269I GAATGC 4 cut(s) 865, 1312, 1723, 1759
MwoI GCNNNNNNNGC 1 cut(s) 1441
NcoI CCATGG 1 cut(s) 1591
NdeII GATC 3 cut(s) 1016, 1145, 1582
NlaIV GGNNCC 1 cut(s) 1406
NmeAIII GCCGAG 1 cut(s) 105
NmuCI GTSAC 2 cut(s) 1370, 1455
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 3 cut(s) 274, 815, 1448
NspV TTCGAA 1 cut(s) 415
PaeI GCATGC 1 cut(s) 1448
PciSI GCTCTTC 2 cut(s) 1080, 1317
PctI GAATGC 4 cut(s) 865, 1312, 1723, 1759
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 4 cut(s) 169, 212, 587, 1377
PflMI CCANNNNNTGG 1 cut(s) 1289
PkrI GCNGC 3 cut(s) 853, 1450, 1759
PleI GAGTC 2 cut(s) 572, 1516
PpsI GAGTC 2 cut(s) 572, 1516
PspFI CCCAGC 2 cut(s) 647, 1498
PspN4I GGNNCC 1 cut(s) 1406
PspPI GGNCC 2 cut(s) 115, 1280
PvuII CAGCTG 2 cut(s) 647, 1630
RsaI GTAC 6 cut(s) 404, 810, 821, 876, 1114, 1264
RsaNI GTAC 6 cut(s) 403, 809, 820, 875, 1113, 1263
RseI CAYNNNNRTG 2 cut(s) 765, 909
SapI GCTCTTC 2 cut(s) 1080, 1317
SaqAI TTAA 7 cut(s) 461, 578, 732, 879, 1203, 1653, 1661
SatI GCNGC 3 cut(s) 852, 1449, 1758
Sau3AI GATC 3 cut(s) 1016, 1145, 1582
Sau96I GGNCC 2 cut(s) 115, 1280
SchI GAGTC 2 cut(s) 573, 1517
SfaNI GCATC 4 cut(s) 912, 1092, 1099, 1276
SfuI TTCGAA 1 cut(s) 415
SinI GGWCC 1 cut(s) 1280
SmiMI CAYNNNNRTG 2 cut(s) 765, 909
SmlI CTYRAG 4 cut(s) 5, 856, 926, 1038
SmoI CTYRAG 4 cut(s) 5, 856, 926, 1038
SphI GCATGC 1 cut(s) 1448
Sse9I AATT 6 cut(s) 417, 936, 1053, 1208, 1460, 1549
SsiI CCGC 2 cut(s) 149, 815
SspMI CTAG 6 cut(s) 68, 93, 321, 329, 1004, 1397
StyI CCWWGG 2 cut(s) 67, 1591
TaaI ACNGT 4 cut(s) 431, 556, 1567, 1671
TaiI ACGT 2 cut(s) 988, 1071
TaqI TCGA 5 cut(s) 415, 590, 920, 1015, 1713
TaqII GACCGA 2 cut(s) 947, 1389
TasI AATT 6 cut(s) 417, 936, 1053, 1208, 1460, 1549
TatI WGTACW 1 cut(s) 819
TfiI GAWTC 4 cut(s) 169, 212, 587, 1377
Tru1I TTAA 7 cut(s) 461, 578, 732, 879, 1203, 1653, 1661
Tru9I TTAA 7 cut(s) 461, 578, 732, 879, 1203, 1653, 1661
TscAI CASTG 2 cut(s) 436, 1770
TseFI GTSAC 2 cut(s) 1370, 1455
TseI GCWGC 3 cut(s) 851, 1448, 1757
Tsp45I GTSAC 2 cut(s) 1370, 1455
TspDTI ATGAA 4 cut(s) 123, 670, 1453, 1591
TspGWI ACGGA 1 cut(s) 205
TspRI CASTG 2 cut(s) 436, 1770
Van91I CCANNNNNTGG 1 cut(s) 1289
VpaK11BI GGWCC 1 cut(s) 1280
XapI RAATTY 2 cut(s) 1208, 1549
XbaI TCTAGA 2 cut(s) 92, 1396
XceI RCATGY 3 cut(s) 274, 815, 1448
XmaJI CCTAGG 1 cut(s) 67
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 6 cut(s) 68, 93, 321, 329, 1004, 1397
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.