AT5G35090

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011070
Physical Location & Seq
Forward (+)
13358370 .. 13359336
967 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35090.1

Sequence Viewer

Length: 480 bp
ATGGGAAGCGAAACAATGACCAACTTGTCTCTACAAAAGAAACTCAAATCAAGGTTTTGCATCGCCGGATGTTTTCGTACGACCAATCATCACCACCACGACAACATTCCCGACGACCTACCATCATCCCCGGTCACCACCGAGAAATCTACACAAAGCCCACATGGTGGTGGAATGAAGACCAAATCTCCAAGACTCACTAGGACGTTGTCCAAGTCACATGAAAAATGTAAGAGTCTGATCCATCGAATGGGCGGTGGAGTAGGTGGCGTTGGCGGTCACGGGAAGCATATTCGCCGCCATACTGCTGACTTTCATTACGATCCGTCGAGCTATGCGCTTAACTTTGATAAAGGAGATGAGGATGACAATATCGACCGTTTTCCGCTACGTAACTTCTCCGCTAGGCTGCCTCATTCACCGCCTTCTTCGGCCAAGGCCGCGGATTCTTCGTATACGGTTCAAAACCTTTTGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.55

Weight (kDa)

9.64

Isoelectric Point (pI)

60.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 25
AccB7I CCANNNNNTGG 2 cut(s) 167, 250
AccI GTMKAC 1 cut(s) 455
AccII CGCG 1 cut(s) 443
AciI CCGC 9 cut(s) 255, 276, 298, 386, 402, 422, 441, 443, 475
AclWI GGATC 2 cut(s) 235, 317
AcoI YGGCCR 1 cut(s) 432
AdeI CACNNNGTG 1 cut(s) 167
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 2 cut(s) 167, 250
AgsI TTSAA 1 cut(s) 464
AluBI AGCT 1 cut(s) 333
AluI AGCT 1 cut(s) 333
Alw26I GTCTC 1 cut(s) 33
AlwI GGATC 2 cut(s) 235, 317
AoxI GGCC 2 cut(s) 432, 438
ApeKI GCWGC 1 cut(s) 409
AspLEI GCGC 1 cut(s) 340
AsuC2I CCSGG 1 cut(s) 131
AsuHPI GGTGA 3 cut(s) 83, 127, 411
BbsI GAAGAC 1 cut(s) 185
BbvI GCAGC 1 cut(s) 396
BccI CCATC 2 cut(s) 130, 252
BcnI CCSGG 1 cut(s) 131
BcoDI GTCTC 1 cut(s) 33
BfaI CTAG 2 cut(s) 201, 405
BisI GCNGC 3 cut(s) 298, 410, 441
BlsI GCNGC 3 cut(s) 299, 411, 442
Bme1390I CCNGG 1 cut(s) 131
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 185
BpuMI CCSGG 1 cut(s) 131
BsaAI YACGTR 1 cut(s) 392
BsaJI CCNNGG 3 cut(s) 129, 435, 441
BsaXI ACNNNNNCTCC 2 cut(s) 172, 202
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 250
BseDI CCNNGG 3 cut(s) 129, 435, 441
BseGI GGATG 3 cut(s) 74, 125, 370
BseLI CCNNNNNNNGG 2 cut(s) 167, 250
BseXI GCAGC 1 cut(s) 396
Bsh1236I CGCG 1 cut(s) 443
Bsh1285I CGRYCG 1 cut(s) 379
BshFI GGCC 2 cut(s) 434, 440
BsiEI CGRYCG 1 cut(s) 379
BsiSI CCGG 2 cut(s) 66, 131
BsiWI CGTACG 1 cut(s) 77
BslI CCNNNNNNNGG 2 cut(s) 167, 250
BsmAI GTCTC 1 cut(s) 33
BsnI GGCC 2 cut(s) 434, 440
Bsp143I GATC 2 cut(s) 240, 322
BspACI CCGC 9 cut(s) 255, 276, 298, 386, 402, 422, 441, 443, 475
BspANI GGCC 2 cut(s) 434, 440
BspFNI CGCG 1 cut(s) 443
BspPI GGATC 2 cut(s) 235, 317
BssECI CCNNGG 3 cut(s) 129, 435, 441
BssMI GATC 2 cut(s) 240, 322
BssNAI GTATAC 1 cut(s) 456
BssT1I CCWWGG 1 cut(s) 435
Bst1107I GTATAC 1 cut(s) 456
Bst4CI ACNGT 2 cut(s) 380, 460
BstBAI YACGTR 1 cut(s) 392
BstDSI CCRYGG 1 cut(s) 441
BstEII GGTNACC 1 cut(s) 133
BstF5I GGATG 3 cut(s) 74, 125, 370
BstFNI CGCG 1 cut(s) 443
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 2 cut(s) 243, 325
BstMAI GTCTC 1 cut(s) 33
BstMBI GATC 2 cut(s) 240, 322
BstMCI CGRYCG 1 cut(s) 379
BstMWI GCNNNNNNNGC 1 cut(s) 440
BstPI GGTNACC 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 129
BstSNI TACGTA 1 cut(s) 392
BstUI CGCG 1 cut(s) 443
BstV1I GCAGC 1 cut(s) 396
BstV2I GAAGAC 1 cut(s) 185
BstZ17I GTATAC 1 cut(s) 456
BsuRI GGCC 2 cut(s) 434, 440
BtgI CCRYGG 1 cut(s) 441
BtgZI GCGATG 1 cut(s) 46
BtsCI GGATG 3 cut(s) 74, 125, 370
CfoI GCGC 1 cut(s) 340
Cfr42I CCGCGG 1 cut(s) 444
Csp6I GTAC 1 cut(s) 78
CviAII CATG 2 cut(s) 164, 221
CviJI RGCY 5 cut(s) 159, 333, 409, 434, 440
CviKI_1 RGCY 5 cut(s) 159, 333, 409, 434, 440
CviQI GTAC 1 cut(s) 78
DpnI GATC 2 cut(s) 242, 324
DpnII GATC 2 cut(s) 240, 322
DraIII CACNNNGTG 1 cut(s) 167
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 1 cut(s) 432
Eco105I TACGTA 1 cut(s) 392
Eco130I CCWWGG 1 cut(s) 435
Eco91I GGTNACC 1 cut(s) 133
EcoO65I GGTNACC 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 2 cut(s) 167, 224
FaiI YATR 6 cut(s) 165, 222, 291, 303, 336, 456
FatI CATG 2 cut(s) 163, 220
FblI GTMKAC 1 cut(s) 455
Fnu4HI GCNGC 3 cut(s) 298, 410, 441
FokI GGATG 3 cut(s) 81, 112, 377
Fsp4HI GCNGC 3 cut(s) 298, 410, 441
FspBI CTAG 2 cut(s) 201, 405
GlaI GCGC 1 cut(s) 339
GluI GCNGC 3 cut(s) 298, 410, 441
HaeIII GGCC 2 cut(s) 434, 440
HapII CCGG 2 cut(s) 66, 131
HhaI GCGC 1 cut(s) 340
Hin1II CATG 2 cut(s) 167, 224
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HinfI GANTC 3 cut(s) 195, 235, 446
HpaII CCGG 2 cut(s) 66, 131
HphI GGTGA 3 cut(s) 83, 127, 411
Hpy166II GTNNAC 1 cut(s) 456
Hpy188I TCNGA 1 cut(s) 240
Hpy188III TCNNGA 1 cut(s) 110
Hpy8I GTNNAC 1 cut(s) 456
Hpy99I CGWCG 2 cut(s) 116, 331
HpyAV CCTTC 1 cut(s) 435
HpyCH4III ACNGT 2 cut(s) 380, 460
HpyCH4IV ACGT 2 cut(s) 206, 391
HpyCH4V TGCA 1 cut(s) 60
HpyF10VI GCNNNNNNNGC 1 cut(s) 440
HpySE526I ACGT 2 cut(s) 206, 391
Hsp92II CATG 2 cut(s) 167, 224
HspAI GCGC 1 cut(s) 338
KspI CCGCGG 1 cut(s) 444
Kzo9I GATC 2 cut(s) 240, 322
LpnPI CCDG 2 cut(s) 79, 144
Lsp1109I GCAGC 1 cut(s) 396
LweI GCATC 1 cut(s) 69
MaeI CTAG 2 cut(s) 201, 405
MaeII ACGT 2 cut(s) 206, 391
MaeIII GTNAC 4 cut(s) 133, 216, 278, 392
MalI GATC 2 cut(s) 242, 324
MboI GATC 2 cut(s) 240, 322
MboII GAAGA 3 cut(s) 190, 420, 441
MlyI GAGTC 2 cut(s) 189, 244
MnlI CCTC 2 cut(s) 355, 423
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 168
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 2 cut(s) 66, 131
MspR9I CCNGG 1 cut(s) 131
MvnI CGCG 1 cut(s) 443
MwoI GCNNNNNNNGC 1 cut(s) 440
NciI CCSGG 1 cut(s) 131
NdeII GATC 2 cut(s) 240, 322
NlaIII CATG 2 cut(s) 167, 224
NmuCI GTSAC 3 cut(s) 133, 216, 278
PfeI GAWTC 1 cut(s) 446
Pfl23II CGTACG 1 cut(s) 77
PflFI GACNNNGTC 1 cut(s) 208
PflMI CCANNNNNTGG 2 cut(s) 167, 250
PkrI GCNGC 3 cut(s) 299, 411, 442
PleI GAGTC 2 cut(s) 189, 243
PpsI GAGTC 2 cut(s) 189, 243
Ppu21I YACGTR 1 cut(s) 392
PspEI GGTNACC 1 cut(s) 133
PspLI CGTACG 1 cut(s) 77
PsyI GACNNNGTC 1 cut(s) 208
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 168
SacII CCGCGG 1 cut(s) 444
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 3 cut(s) 298, 410, 441
Sau3AI GATC 2 cut(s) 240, 322
SchI GAGTC 2 cut(s) 189, 244
ScrFI CCNGG 1 cut(s) 131
SetI ASST 7 cut(s) 56, 120, 209, 268, 335, 394, 471
SfaNI GCATC 1 cut(s) 69
Sfr303I CCGCGG 1 cut(s) 444
SgrBI CCGCGG 1 cut(s) 444
SmiMI CAYNNNNRTG 1 cut(s) 168
SnaBI TACGTA 1 cut(s) 392
SsiI CCGC 9 cut(s) 255, 276, 298, 386, 402, 422, 441, 443, 475
SspMI CTAG 2 cut(s) 201, 405
StyD4I CCNGG 1 cut(s) 129
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 2 cut(s) 380, 460
TaiI ACGT 2 cut(s) 209, 394
TaqI TCGA 3 cut(s) 247, 329, 375
TauI GCSGC 2 cut(s) 300, 443
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TseFI GTSAC 3 cut(s) 133, 216, 278
TseI GCWGC 1 cut(s) 409
Tsp45I GTSAC 3 cut(s) 133, 216, 278
TspDTI ATGAA 3 cut(s) 191, 237, 305
TspGWI ACGGA 1 cut(s) 315
Tth111I GACNNNGTC 1 cut(s) 208
Van91I CCANNNNNTGG 2 cut(s) 167, 250
XmiI GTMKAC 1 cut(s) 455
XspI CTAG 2 cut(s) 201, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.