AT5G38510

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006220
Physical Location & Seq
Reverse (-)
15417636 .. 15420638
3003 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G38510.2

Sequence Viewer

Length: 1305 bp
ATGGCACTGTTTCCTCTTCACCATGAAGTCCCTTGTAAAGGAGAAGTATTGTTTGATTCAAATGGTTTAAGACGTTTTTCGTCTGGTCTGAAACATAGAACAATGGCTGAAGCAACCACGCTTGGCAGGGATTGTAGGATGAAGAGTTATATGAAATCAATCCCGTATTGTCGTTCACCAAGAAGAAGGTTATGTTTAGTTAGAGCGTCATCAGAGAACAAGATTACCAAACAGAGACTTAAACTGTTGGATTCTTACTTTGGGAAACTTCAAAACGATGATGAGAAGCCTTCTATCTCAACAGGAGATGATATTGATCGCAAAGCTGAACTCAATGTGAATGAAGAGTTGGATTCTTTGAGTGCTTATCTTGACAAACTCCAAAAAGATGCAAAATCTAAAGGTTTAGTCTCGTCCACCTTGGACGTGGTAAAATCAGAAGGCGGTTCAGTAGCAAGCAAATTGAGAAAGACTGGTATTGAAAACAATAATAGTCCTTTCCAGCAGTTTGATGATGAAGATCAAGCAGAAGATACATTGAACTTCTATGCTGTGAGCATATTAGCATCCATCAATGTTGGTGTCTGCTTGTTTGAGGCGGCAGCGCCAGTTAGGAACAACAATATGGGACTCTTATCACTCCCTTTGTTATATGGAGCAAAGATAAATGATCTAATCCTTGCTGGAGAATGGTGGAGGCTGGTCACACCCATGTTTCTGCACTCTGGAATTCCTCATGTAGCCCTTAGCTCCTGGGCTCTGCTTACTTTTGGACCAAAAGTCTGCCGTGACTATGGATTATTCACGTTTTGTCTCATTTACATTCTCGGAGGAGTCTCTGGAAATTTCATGAGCTTTCTGCATACAGCAGATCCTACAGTTGGAGGAACTGGACCAGCATTTGCGTTAATAGGAGCTTGGCTCGTTGACCAAAATCAGAACAAAGAGATGATCAAAAGCAACGAATATGAGGACTTGTTTCAGAAGGCCATTATAATGACAGGATTTGGTCTCATATTAAGCCATTTCGGTCCAATTGATGACTGGACAAATCTGGGAGCACTTATAGCAGGGATTGTATACGGGTTTTTCACTTGTCCGGTGCTACAACTTGGTAGCGGCGGAAGTGAAAGGCAAGAAGGGATTGTGACGGTTGGACCAGAGAAACAAAACAGCGCTGACCCGTGTAAATCGTTTCTGCTTTTCACAATCTTCGTCGCGGTTATTGTGACTTCTCTGCTACTGATTGGGGACGGTCCATTGGACTTCCCAACGTACGATGATGTTGTGTATTCTCTCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

434

Amino Acids

47.73

Weight (kDa)

5.6

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 229 - 366 3.2e-34 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 995
AccI GTMKAC 1 cut(s) 1080
AccII CGCG 1 cut(s) 1220
AciI CCGC 5 cut(s) 444, 599, 1119, 1122, 1220
AclWI GGATC 1 cut(s) 866
AcsI RAATTY 2 cut(s) 729, 844
AcuI CTGAAG 1 cut(s) 129
AfaI GTAC 1 cut(s) 1277
AfeI AGCGCT 1 cut(s) 1177
AfiI CCNNNNNNNGG 2 cut(s) 38, 881
AgsI TTSAA 4 cut(s) 60, 272, 482, 541
AhdI GACNNNNNGTC 1 cut(s) 779
AjiI CACGTC 1 cut(s) 427
AjnI CCWGG 1 cut(s) 752
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AluBI AGCT 4 cut(s) 326, 750, 855, 917
AluI AGCT 4 cut(s) 326, 750, 855, 917
Alw21I GWGCWC 1 cut(s) 1063
Alw26I GTCTC 5 cut(s) 229, 415, 818, 841, 1016
AlwI GGATC 1 cut(s) 866
Aor51HI AGCGCT 1 cut(s) 1177
AoxI GGCC 1 cut(s) 987
ApeKI GCWGC 1 cut(s) 602
ApoI RAATTY 2 cut(s) 729, 844
AspLEI GCGC 2 cut(s) 607, 1178
AspS9I GGNCC 5 cut(s) 773, 893, 1031, 1157, 1256
AsuHPI GGTGA 2 cut(s) 11, 168
AvaII GGWCC 5 cut(s) 773, 893, 1031, 1157, 1256
BanII GRGCYC 1 cut(s) 760
Bbv12I GWGCWC 1 cut(s) 1063
BbvI GCAGC 1 cut(s) 614
BccI CCATC 1 cut(s) 578
BceAI ACGGC 1 cut(s) 771
BciT130I CCWGG 1 cut(s) 754
BclI TGATCA 1 cut(s) 951
BcoDI GTCTC 5 cut(s) 229, 415, 818, 841, 1016
BfmI CTRYAG 1 cut(s) 876
BfoI RGCGCY 2 cut(s) 608, 1179
BisI GCNGC 3 cut(s) 600, 603, 1120
BlsI GCNGC 3 cut(s) 601, 604, 1121
Bme1390I CCNGG 1 cut(s) 754
Bme18I GGWCC 5 cut(s) 773, 893, 1031, 1157, 1256
BmeRI GACNNNNNGTC 1 cut(s) 779
BmgBI CACGTC 1 cut(s) 427
BmgT120I GGNCC 5 cut(s) 773, 893, 1031, 1157, 1256
BmrFI CCNGG 1 cut(s) 754
BmsI GCATC 2 cut(s) 379, 575
BplI GAGNNNNNCTC 2 cut(s) 906, 938
BpmI CTGGAG 1 cut(s) 705
Bpu10I CCTNAGC 1 cut(s) 746
BsaBI GATNNNNATC 2 cut(s) 315, 519
BsaI GGTCTC 1 cut(s) 1016
BsaJI CCNNGG 2 cut(s) 420, 753
BsaWI WCCGGW 1 cut(s) 1099
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 881
Bse1I ACTGG 4 cut(s) 478, 608, 895, 1049
Bse8I GATNNNNATC 2 cut(s) 315, 519
BseBI CCWGG 1 cut(s) 754
BseDI CCNNGG 2 cut(s) 420, 753
BseGI GGATG 2 cut(s) 144, 566
BseJI GATNNNNATC 2 cut(s) 315, 519
BseLI CCNNNNNNNGG 2 cut(s) 38, 881
BseNI ACTGG 4 cut(s) 478, 608, 895, 1049
BseRI GAGGAG 1 cut(s) 846
BseXI GCAGC 1 cut(s) 614
BsgI GTGCAG 1 cut(s) 704
Bsh1236I CGCG 1 cut(s) 1220
BshFI GGCC 1 cut(s) 989
BsiHKAI GWGCWC 1 cut(s) 1063
BsiSI CCGG 1 cut(s) 1100
BsiWI CGTACG 1 cut(s) 1275
BslFI GGGAC 3 cut(s) 14, 642, 1265
BslI CCNNNNNNNGG 2 cut(s) 38, 881
BsmAI GTCTC 5 cut(s) 229, 415, 818, 841, 1016
BsmFI GGGAC 3 cut(s) 14, 642, 1265
BsnI GGCC 1 cut(s) 989
Bso31I GGTCTC 1 cut(s) 1016
Bsp1286I GDGCHC 2 cut(s) 760, 1063
Bsp143I GATC 5 cut(s) 316, 520, 670, 871, 951
BspACI CCGC 5 cut(s) 444, 599, 1119, 1122, 1220
BspANI GGCC 1 cut(s) 989
BspFNI CGCG 1 cut(s) 1220
BspHI TCATGA 1 cut(s) 849
BspPI GGATC 1 cut(s) 866
BspTNI GGTCTC 1 cut(s) 1016
BsrI ACTGG 4 cut(s) 478, 608, 895, 1049
BssECI CCNNGG 2 cut(s) 420, 753
BssMI GATC 5 cut(s) 316, 520, 670, 871, 951
BssNAI GTATAC 1 cut(s) 1081
BssT1I CCWWGG 1 cut(s) 420
Bst1107I GTATAC 1 cut(s) 1081
Bst2UI CCWGG 1 cut(s) 754
Bst4CI ACNGT 5 cut(s) 9, 246, 880, 1153, 1256
Bst6I CTCTTC 3 cut(s) 21, 137, 339
BstC8I GCNNGC 1 cut(s) 457
BstDEI CTNAG 1 cut(s) 746
BstENI CCTNNNNNAGG 1 cut(s) 36
BstF5I GGATG 2 cut(s) 144, 566
BstFNI CGCG 1 cut(s) 1220
BstH2I RGCGCY 2 cut(s) 608, 1179
BstHHI GCGC 2 cut(s) 607, 1178
BstKTI GATC 5 cut(s) 319, 523, 673, 874, 954
BstMAI GTCTC 5 cut(s) 229, 415, 818, 841, 1016
BstMBI GATC 5 cut(s) 316, 520, 670, 871, 951
BstMWI GCNNNNNNNGC 1 cut(s) 1067
BstNI CCWGG 1 cut(s) 754
BstSCI CCNGG 1 cut(s) 752
BstSFI CTRYAG 1 cut(s) 876
BstUI CGCG 1 cut(s) 1220
BstV1I GCAGC 1 cut(s) 614
BstX2I RGATCY 1 cut(s) 871
BstYI RGATCY 1 cut(s) 871
BstZ17I GTATAC 1 cut(s) 1081
BsuRI GGCC 1 cut(s) 989
BtrI CACGTC 1 cut(s) 427
BtsCI GGATG 2 cut(s) 144, 566
BtsIMutI CAGTG 1 cut(s) 5
Cac8I GCNNGC 1 cut(s) 457
CciI TCATGA 1 cut(s) 849
CfoI GCGC 2 cut(s) 607, 1178
Cfr13I GGNCC 5 cut(s) 773, 893, 1031, 1157, 1256
CseI GACGC 1 cut(s) 195
Csp6I GTAC 1 cut(s) 1276
CviAII CATG 4 cut(s) 23, 712, 737, 850
CviQI GTAC 1 cut(s) 1276
DdeI CTNAG 1 cut(s) 746
DpnI GATC 5 cut(s) 318, 522, 672, 873, 953
DpnII GATC 5 cut(s) 316, 520, 670, 871, 951
DriI GACNNNNNGTC 1 cut(s) 779
Eam1104I CTCTTC 3 cut(s) 21, 137, 339
Eam1105I GACNNNNNGTC 1 cut(s) 779
EarI CTCTTC 3 cut(s) 21, 137, 339
EciI GGCGGA 1 cut(s) 1137
Eco130I CCWWGG 1 cut(s) 420
Eco24I GRGCYC 1 cut(s) 760
Eco31I GGTCTC 1 cut(s) 1016
Eco47I GGWCC 5 cut(s) 773, 893, 1031, 1157, 1256
Eco47III AGCGCT 1 cut(s) 1177
Eco57I CTGAAG 1 cut(s) 129
EcoNI CCTNNNNNAGG 1 cut(s) 36
EcoRI GAATTC 1 cut(s) 729
EcoRII CCWGG 1 cut(s) 752
EcoT14I CCWWGG 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 760
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 4 cut(s) 26, 715, 740, 853
FaqI GGGAC 3 cut(s) 14, 642, 1265
FatI CATG 4 cut(s) 22, 711, 736, 849
FbaI TGATCA 1 cut(s) 951
FblI GTMKAC 1 cut(s) 1080
Fnu4HI GCNGC 3 cut(s) 600, 603, 1120
FokI GGATG 2 cut(s) 151, 553
FriOI GRGCYC 1 cut(s) 760
Fsp4HI GCNGC 3 cut(s) 600, 603, 1120
GlaI GCGC 2 cut(s) 606, 1177
GluI GCNGC 3 cut(s) 600, 603, 1120
GsuI CTGGAG 1 cut(s) 705
HaeII RGCGCY 2 cut(s) 608, 1179
HaeIII GGCC 1 cut(s) 989
HapII CCGG 1 cut(s) 1100
HgaI GACGC 1 cut(s) 195
HhaI GCGC 2 cut(s) 607, 1178
Hin1II CATG 4 cut(s) 26, 715, 740, 853
Hin6I GCGC 2 cut(s) 605, 1176
HinP1I GCGC 2 cut(s) 605, 1176
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HinfI GANTC 5 cut(s) 56, 251, 353, 630, 834
HpaII CCGG 1 cut(s) 1100
HphI GGTGA 2 cut(s) 11, 168
Hpy166II GTNNAC 4 cut(s) 176, 417, 928, 1081
Hpy188I TCNGA 6 cut(s) 90, 214, 439, 830, 939, 984
Hpy188III TCNNGA 4 cut(s) 371, 726, 840, 850
Hpy8I GTNNAC 4 cut(s) 176, 417, 928, 1081
Hpy99I CGWCG 1 cut(s) 1220
HpyAV CCTTC 5 cut(s) 180, 300, 434, 979, 1133
HpyCH4III ACNGT 5 cut(s) 9, 246, 880, 1153, 1256
HpyCH4IV ACGT 4 cut(s) 73, 426, 806, 1274
HpyCH4V TGCA 3 cut(s) 392, 721, 862
HpyF10VI GCNNNNNNNGC 1 cut(s) 1067
HpyF3I CTNAG 1 cut(s) 746
HpySE526I ACGT 4 cut(s) 73, 426, 806, 1274
Hsp92II CATG 4 cut(s) 26, 715, 740, 853
HspAI GCGC 2 cut(s) 605, 1176
Ksp22I TGATCA 1 cut(s) 951
Kzo9I GATC 5 cut(s) 316, 520, 670, 871, 951
LmnI GCTCC 4 cut(s) 656, 755, 914, 1058
Lsp1109I GCAGC 1 cut(s) 614
LweI GCATC 2 cut(s) 379, 575
MaeII ACGT 4 cut(s) 73, 426, 806, 1274
MaeIII GTNAC 4 cut(s) 703, 788, 1147, 1228
MalI GATC 5 cut(s) 318, 522, 672, 873, 953
MboI GATC 5 cut(s) 316, 520, 670, 871, 951
MboII GAAGA 7 cut(s) 8, 154, 195, 356, 530, 542, 1204
MfeI CAATTG 1 cut(s) 1035
MflI RGATCY 1 cut(s) 871
MhlI GDGCHC 2 cut(s) 760, 1063
MluCI AATT 4 cut(s) 461, 729, 844, 1035
MlyI GAGTC 2 cut(s) 624, 843
MmeI TCCRAC 4 cut(s) 228, 330, 862, 1135
MnlI CCTC 7 cut(s) 24, 589, 690, 744, 824, 878, 964
MseI TTAA 4 cut(s) 68, 240, 908, 1019
MslI CAYNNNNRTG 2 cut(s) 710, 995
MspI CCGG 1 cut(s) 1100
MspR9I CCNGG 1 cut(s) 754
MunI CAATTG 1 cut(s) 1035
MvaI CCWGG 1 cut(s) 754
MvnI CGCG 1 cut(s) 1220
MwoI GCNNNNNNNGC 1 cut(s) 1067
NdeII GATC 5 cut(s) 316, 520, 670, 871, 951
NlaIII CATG 4 cut(s) 26, 715, 740, 853
NmuCI GTSAC 4 cut(s) 703, 788, 1147, 1228
PagI TCATGA 1 cut(s) 849
PfeI GAWTC 3 cut(s) 56, 251, 353
Pfl23II CGTACG 1 cut(s) 1275
PkrI GCNGC 3 cut(s) 601, 604, 1121
PleI GAGTC 2 cut(s) 624, 842
PpsI GAGTC 2 cut(s) 624, 842
PsiI TTATAA 1 cut(s) 995
Psp6I CCWGG 1 cut(s) 752
PspGI CCWGG 1 cut(s) 752
PspLI CGTACG 1 cut(s) 1275
PspPI GGNCC 5 cut(s) 773, 893, 1031, 1157, 1256
PsuI RGATCY 1 cut(s) 871
RsaI GTAC 1 cut(s) 1277
RsaNI GTAC 1 cut(s) 1276
RseI CAYNNNNRTG 2 cut(s) 710, 995
SaqAI TTAA 4 cut(s) 68, 240, 908, 1019
SatI GCNGC 3 cut(s) 600, 603, 1120
Sau3AI GATC 5 cut(s) 316, 520, 670, 871, 951
Sau96I GGNCC 5 cut(s) 773, 893, 1031, 1157, 1256
SchI GAGTC 2 cut(s) 624, 843
ScrFI CCNGG 1 cut(s) 754
SduI GDGCHC 2 cut(s) 760, 1063
SfaNI GCATC 2 cut(s) 379, 575
SfcI CTRYAG 1 cut(s) 876
SinI GGWCC 5 cut(s) 773, 893, 1031, 1157, 1256
SmiMI CAYNNNNRTG 2 cut(s) 710, 995
Sse9I AATT 4 cut(s) 461, 729, 844, 1035
SsiI CCGC 5 cut(s) 444, 599, 1119, 1122, 1220
StyD4I CCNGG 1 cut(s) 752
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 5 cut(s) 9, 246, 880, 1153, 1256
TaiI ACGT 4 cut(s) 76, 429, 809, 1277
TaqII GACCGA 1 cut(s) 1019
TasI AATT 4 cut(s) 461, 729, 844, 1035
TauI GCSGC 2 cut(s) 602, 1122
TfiI GAWTC 3 cut(s) 56, 251, 353
Tru1I TTAA 4 cut(s) 68, 240, 908, 1019
Tru9I TTAA 4 cut(s) 68, 240, 908, 1019
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 4 cut(s) 703, 788, 1147, 1228
TseI GCWGC 1 cut(s) 602
Tsp45I GTSAC 4 cut(s) 703, 788, 1147, 1228
TspDTI ATGAA 6 cut(s) 39, 155, 167, 357, 531, 838
TspRI CASTG 1 cut(s) 12
VpaK11BI GGWCC 5 cut(s) 773, 893, 1031, 1157, 1256
XagI CCTNNNNNAGG 1 cut(s) 36
XapI RAATTY 2 cut(s) 729, 844
XcmI CCANNNNNNNNNTGG 2 cut(s) 424, 1041
XmiI GTMKAC 1 cut(s) 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.