RLG00000021373

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006220
Physical Location & Seq
Reverse (-)
74651112 .. 74653133
2022 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021373

Sequence Viewer

Length: 1116 bp
ATGGTTCGGTATGCATCAGATGCTAGAATGAATGAGAAGCAGCTGAGTTCTTTGGATTCCTACTTTGAAAAACTCCAAAATAGCACAACTTTGCCTCCAGAAAATTCAAGCAAGACAACTGAGCCACTCGGTAGAAAAAATGGTCATTTGGGATTAGAGGAGGAGTTGGAGTATCTTAATGCGTATCTTCAAAAACTTAATAAAGATGCAAACCAGATTGAAAAACCAGTTGATCTTGCAACTGGATACAAGATAGTTCCAAAATCATCATCTATCATTCAACAATCCAAAAAAGGTGCTGAGAGGAAACTGGTCAAGAGCTATACAAATACAACTGATGATAATGGTCAAGATAGTGAAATCTCTGATCTCTACCTAGTAAGCATATTGGGTTCTATAAACATTGCAGTTTTTCTGTTTGAGATAGCAAGTCCGGTTAGGAATTCTGACTTGGAGCTTTTTTCTCTTCCATTGTTATATGGAGCAAAGATAAATGATTTGATCCTGGTCGGAGAATGGTGGAGGCTAGTCACACCGATGTTTCTGCATGCTGGACTTATTCATATAGCTATTGGTTCTTGGGGACTAGTTACATTTGGACCTAAGGTGTGCAGAGGCTATGGTTCATTCACATTTTTCTTGATCTATATACTTGGAGGAATCTCTGGCAATTTGATTAGCTTTATCCACACACCAGAACCAACTGTTGGTGGAACAGGACCAATATTTGCCATGATCGGAGCTTGGCTCATTTATGAGACCCAAAACAAGGATATAATTGCAAAGGAAGTCTCAGAGACTATGTTCCAAAAGGCAATAATAACTACAGCTCTTACCTTCACATTGAGCAGTTTTGGTCCAATTGATGACTGGACACATTTTGGAGCTGCATTGACAGGAATAGCATATGGGTTTTTGACATGTCCAAGTCTTCAACTGGACGAAGCATCATCATCTTCAACAAGTGGCCAAGAGGAAGGAATCACACTCGTTAGACGTTATGCTGATCCCTGCAAATCACTTTTTCTCTTCACAGTGTTTGTTCTTGTTCTGAGTTCTCTACTTCTCTTTGTTGAACCTCCACTCAACGCCTTAGCATCAGACTCTTCTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

372

Amino Acids

40.73

Weight (kDa)

5.08

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 171 - 309 1.2e-32 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 707
AclWI GGATC 2 cut(s) 496, 1001
AcoI YGGCCR 1 cut(s) 967
AcsI RAATTY 2 cut(s) 103, 442
AfiI CCNNNNNNNGG 2 cut(s) 707, 769
AflIII ACRYGT 1 cut(s) 920
AgsI TTSAA 8 cut(s) 68, 108, 191, 221, 281, 935, 960, 1076
AhlI ACTAGT 1 cut(s) 586
AjnI CCWGG 1 cut(s) 504
AjuI GAANNNNNNNTTGG 4 cut(s) 434, 466, 690, 722
AluBI AGCT 8 cut(s) 43, 321, 457, 569, 681, 743, 830, 887
AluI AGCT 8 cut(s) 43, 321, 457, 569, 681, 743, 830, 887
Alw26I GTCTC 3 cut(s) 752, 791, 796
AlwI GGATC 2 cut(s) 496, 1001
AoxI GGCC 1 cut(s) 967
ApeKI GCWGC 2 cut(s) 40, 887
ApoI RAATTY 2 cut(s) 103, 442
AspS9I GGNCC 3 cut(s) 599, 719, 857
AvaII GGWCC 3 cut(s) 599, 719, 857
AxyI CCTNAGG 1 cut(s) 603
BalI TGGCCA 1 cut(s) 969
BbsI GAAGAC 1 cut(s) 923
BbvI GCAGC 2 cut(s) 52, 874
BcgI CGANNNNNNTGC 2 cut(s) 526, 560
BciT130I CCWGG 1 cut(s) 506
BciVI GTATCC 1 cut(s) 239
BcoDI GTCTC 3 cut(s) 752, 791, 796
BcuI ACTAGT 1 cut(s) 586
BfaI CTAG 4 cut(s) 24, 377, 527, 587
BfmI CTRYAG 1 cut(s) 825
BfuI GTATCC 1 cut(s) 239
BisI GCNGC 2 cut(s) 41, 888
BlsI GCNGC 2 cut(s) 42, 889
Bme1390I CCNGG 1 cut(s) 506
Bme18I GGWCC 3 cut(s) 599, 719, 857
BmgT120I GGNCC 3 cut(s) 599, 719, 857
BmrFI CCNGG 1 cut(s) 506
BmsI GCATC 5 cut(s) 10, 23, 196, 956, 1106
BpiI GAAGAC 1 cut(s) 923
BplI GAGNNNNNCTC 2 cut(s) 732, 764
BpmI CTGGAG 1 cut(s) 81
Bpu10I CCTNAGC 1 cut(s) 1093
BsaI GGTCTC 1 cut(s) 752
BsaWI WCCGGW 1 cut(s) 433
BsaXI ACNNNNNCTCC 4 cut(s) 79, 109, 155, 185
Bsc4I CCNNNNNNNGG 2 cut(s) 707, 769
Bse1I ACTGG 5 cut(s) 227, 247, 315, 875, 942
Bse21I CCTNAGG 1 cut(s) 603
Bse3DI GCAATG 1 cut(s) 402
BseBI CCWGG 1 cut(s) 506
BseLI CCNNNNNNNGG 2 cut(s) 707, 769
BseMI GCAATG 1 cut(s) 402
BseMII CTCAG 5 cut(s) 35, 111, 291, 807, 1043
BseNI ACTGG 5 cut(s) 227, 247, 315, 875, 942
BseRI GAGGAG 2 cut(s) 173, 176
BseXI GCAGC 2 cut(s) 52, 874
BsgI GTGCAG 1 cut(s) 631
BshFI GGCC 1 cut(s) 969
BsiSI CCGG 1 cut(s) 434
BslFI GGGAC 1 cut(s) 597
BslI CCNNNNNNNGG 2 cut(s) 707, 769
BsmAI GTCTC 3 cut(s) 752, 791, 796
BsmFI GGGAC 1 cut(s) 597
BsnI GGCC 1 cut(s) 969
Bso31I GGTCTC 1 cut(s) 752
Bsp143I GATC 6 cut(s) 232, 367, 501, 642, 735, 1006
BspANI GGCC 1 cut(s) 969
BspCNI CTCAG 5 cut(s) 36, 112, 292, 806, 1044
BspPI GGATC 2 cut(s) 496, 1001
BspTNI GGTCTC 1 cut(s) 752
BsrDI GCAATG 1 cut(s) 402
BsrI ACTGG 5 cut(s) 227, 247, 315, 875, 942
BssMI GATC 6 cut(s) 232, 367, 501, 642, 735, 1006
Bst2UI CCWGG 1 cut(s) 506
Bst4CI ACNGT 2 cut(s) 706, 1036
Bst6I CTCTTC 3 cut(s) 471, 1034, 1111
BstAPI GCANNNNNTGC 1 cut(s) 20
BstC8I GCNNGC 1 cut(s) 549
BstDEI CTNAG 7 cut(s) 44, 120, 300, 603, 793, 1052, 1093
BstKTI GATC 6 cut(s) 235, 370, 504, 645, 738, 1009
BstMAI GTCTC 3 cut(s) 752, 791, 796
BstMBI GATC 6 cut(s) 232, 367, 501, 642, 735, 1006
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 1 cut(s) 506
BstNSI RCATGY 2 cut(s) 551, 924
BstSCI CCNGG 1 cut(s) 504
BstSFI CTRYAG 1 cut(s) 825
BstV1I GCAGC 2 cut(s) 52, 874
BstV2I GAAGAC 1 cut(s) 923
Bsu36I CCTNAGG 1 cut(s) 603
BsuI GTATCC 1 cut(s) 239
BsuRI GGCC 1 cut(s) 969
BtsIMutI CAGTG 1 cut(s) 1041
Cac8I GCNNGC 1 cut(s) 549
Cfr13I GGNCC 3 cut(s) 599, 719, 857
CviAII CATG 3 cut(s) 548, 733, 921
DdeI CTNAG 7 cut(s) 44, 120, 300, 603, 793, 1052, 1093
DpnI GATC 6 cut(s) 234, 369, 503, 644, 737, 1008
DpnII GATC 6 cut(s) 232, 367, 501, 642, 735, 1006
EaeI YGGCCR 1 cut(s) 967
Eam1104I CTCTTC 3 cut(s) 471, 1034, 1111
EarI CTCTTC 3 cut(s) 471, 1034, 1111
Eco31I GGTCTC 1 cut(s) 752
Eco47I GGWCC 3 cut(s) 599, 719, 857
Eco81I CCTNAGG 1 cut(s) 603
EcoRI GAATTC 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 504
EcoT22I ATGCAT 1 cut(s) 16
FaeI CATG 3 cut(s) 551, 736, 924
FaqI GGGAC 1 cut(s) 597
FatI CATG 3 cut(s) 547, 732, 920
FauNDI CATATG 1 cut(s) 907
Fnu4HI GCNGC 2 cut(s) 41, 888
Fsp4HI GCNGC 2 cut(s) 41, 888
FspBI CTAG 4 cut(s) 24, 377, 527, 587
GluI GCNGC 2 cut(s) 41, 888
GsuI CTGGAG 1 cut(s) 81
HaeIII GGCC 1 cut(s) 969
HapII CCGG 1 cut(s) 434
Hin1II CATG 3 cut(s) 551, 736, 924
HinfI GANTC 4 cut(s) 56, 660, 981, 1103
HpaII CCGG 1 cut(s) 434
Hpy188I TCNGA 8 cut(s) 19, 367, 448, 512, 740, 796, 1053, 1102
Hpy188III TCNNGA 4 cut(s) 98, 316, 350, 640
HpyAV CCTTC 2 cut(s) 847, 971
HpyCH4III ACNGT 2 cut(s) 706, 1036
HpyCH4IV ACGT 1 cut(s) 997
HpyCH4V TGCA 9 cut(s) 14, 209, 239, 407, 547, 612, 782, 890, 1014
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 7 cut(s) 44, 120, 300, 603, 793, 1052, 1093
HpySE526I ACGT 1 cut(s) 997
Hsp92II CATG 3 cut(s) 551, 736, 924
Kzo9I GATC 6 cut(s) 232, 367, 501, 642, 735, 1006
LmnI GCTCC 4 cut(s) 454, 482, 740, 884
Lsp1109I GCAGC 2 cut(s) 52, 874
LweI GCATC 5 cut(s) 10, 23, 196, 956, 1106
MaeI CTAG 4 cut(s) 24, 377, 527, 587
MaeII ACGT 1 cut(s) 997
MaeIII GTNAC 2 cut(s) 529, 589
MalI GATC 6 cut(s) 234, 369, 503, 644, 737, 1008
MboI GATC 6 cut(s) 232, 367, 501, 642, 735, 1006
MboII GAAGA 6 cut(s) 179, 458, 923, 948, 1021, 1098
MfeI CAATTG 1 cut(s) 861
MlsI TGGCCA 1 cut(s) 969
MluCI AATT 5 cut(s) 103, 442, 670, 777, 861
MluNI TGGCCA 1 cut(s) 969
MlyI GAGTC 1 cut(s) 1097
MmeI TCCRAC 2 cut(s) 147, 490
MnlI CCTC 9 cut(s) 105, 151, 154, 297, 516, 608, 650, 967, 1089
Mox20I TGGCCA 1 cut(s) 969
Mph1103I ATGCAT 1 cut(s) 16
MscI TGGCCA 1 cut(s) 969
MseI TTAA 2 cut(s) 177, 198
MslI CAYNNNNRTG 1 cut(s) 536
Msp20I TGGCCA 1 cut(s) 969
MspA1I CMGCKG 1 cut(s) 43
MspI CCGG 1 cut(s) 434
MspR9I CCNGG 1 cut(s) 506
MunI CAATTG 1 cut(s) 861
MvaI CCWGG 1 cut(s) 506
MwoI GCNNNNNNNGC 1 cut(s) 20
NdeI CATATG 1 cut(s) 907
NdeII GATC 6 cut(s) 232, 367, 501, 642, 735, 1006
NlaIII CATG 3 cut(s) 551, 736, 924
NmuCI GTSAC 1 cut(s) 529
NsiI ATGCAT 1 cut(s) 16
NspI RCATGY 2 cut(s) 551, 924
PaeI GCATGC 1 cut(s) 551
PciI ACATGT 1 cut(s) 920
PfeI GAWTC 3 cut(s) 56, 660, 981
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 2 cut(s) 42, 889
PleI GAGTC 1 cut(s) 1097
PpsI GAGTC 1 cut(s) 1097
PscI ACATGT 1 cut(s) 920
Psp6I CCWGG 1 cut(s) 504
PspGI CCWGG 1 cut(s) 504
PspPI GGNCC 3 cut(s) 599, 719, 857
PvuII CAGCTG 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 536
SaqAI TTAA 2 cut(s) 177, 198
SatI GCNGC 2 cut(s) 41, 888
Sau3AI GATC 6 cut(s) 232, 367, 501, 642, 735, 1006
Sau96I GGNCC 3 cut(s) 599, 719, 857
SchI GAGTC 1 cut(s) 1097
ScrFI CCNGG 1 cut(s) 506
SfaNI GCATC 5 cut(s) 10, 23, 196, 956, 1106
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 3 cut(s) 599, 719, 857
SmiMI CAYNNNNRTG 1 cut(s) 536
SpeI ACTAGT 1 cut(s) 586
SphI GCATGC 1 cut(s) 551
Sse9I AATT 5 cut(s) 103, 442, 670, 777, 861
SspI AATATT 1 cut(s) 726
SspMI CTAG 4 cut(s) 24, 377, 527, 587
StyD4I CCNGG 1 cut(s) 504
TaaI ACNGT 2 cut(s) 706, 1036
TaiI ACGT 1 cut(s) 1000
TasI AATT 5 cut(s) 103, 442, 670, 777, 861
TfiI GAWTC 3 cut(s) 56, 660, 981
Tru1I TTAA 2 cut(s) 177, 198
Tru9I TTAA 2 cut(s) 177, 198
TscAI CASTG 1 cut(s) 1041
TseFI GTSAC 1 cut(s) 529
TseI GCWGC 2 cut(s) 40, 887
Tsp45I GTSAC 1 cut(s) 529
TspDTI ATGAA 3 cut(s) 44, 551, 615
TspRI CASTG 1 cut(s) 1041
Van91I CCANNNNNTGG 1 cut(s) 707
VpaK11BI GGWCC 3 cut(s) 599, 719, 857
XapI RAATTY 2 cut(s) 103, 442
XceI RCATGY 2 cut(s) 551, 924
XcmI CCANNNNNNNNNTGG 1 cut(s) 867
XspI CTAG 4 cut(s) 24, 377, 527, 587
Zsp2I ATGCAT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.