FvH4_6g45321

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006220
Physical Location & Seq
Reverse (-)
34804638 .. 34807394
2757 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45321.t3

Sequence Viewer

Length: 1260 bp
ATGGCTTTGGCTCCGGTATGCTACAGGGTACCATGCAAAGGCCATATTCAACCGATACATTATGTAATAAGAGGACCAAGTTCATCTAAAGGACTAGAAAGCAGTCTAAGATGCAGTTTAGCACATGATTCACAAGAACAAAATCGTGGGACGGTTCGGTATGCATCAGATGCTAGAACGAATGAGAAGCAGCTGAGTTCTTTAGATTCTTACTTTGGGAAACTCCAAAATAGCACAAGTCTGCCTCCAGAAAATGTAAGCAAGACGAGGGAACCGCTCGGTAGAAAAACTGATCGTTTGGGATTGAAGGAGGAGTTGGATTATCTTGATGCTTATCTTGGAAAACTTAATGAAGATGCAAATCAGATTAATAAACCAGTTGATGTTTCTACTGGAGACAATAGAGTTCCAAAATCATTATCTATCATTCAAGAATCCGAAAACGTTGCCGAGAGGAAACTGGTCAAGAGCTATACAAGCACAGCTGATGATAATGGTCAAGATAGTGAGATCTCTGATCTCTACCTAATAAGCATATTGGGATCTATCAACATTGCAGTTTTTCTGTTTGAGATAGCAAGTCCAGTTAAGAATTCTGAGCTCTTTTCTCTTCCATTGTTATATGGAGCAAAGATAAATGATTTGATCATGGTCGGAGAATGGTGGAGGCTAGTCACACCGATGTTTCTGCACGCCGGACTCATTCATATAGCTATCGGTTCTTGGGGACTTGTTACATTCGGACCTAAGGTGTGCAGAGGCTATGGTTCATTCACATTTTTCTTGATCTATATGCTTGGAGGAATCTCTGGGAATTTGATTAGCTTTCTTCACACACCAGAACCAACTGTCGGTGGAACTGGACCGATATTTGCCATGATTGGAGCTTGGCTCACCTATGAGACCCAAAACAAAGATATGATTGCAAAGGAAGTCTCAGAGAGTATGTTCCAGAAGGCAATAATAACTACAGCTCTTACCTTCACATTGAGCAGTTTTGGTCCTATTGATGACTGGACACATTTTGGAGCTGCATTGACAGGAATAGCATATGGGTTTTTGACATGCCCTAGTCTTCAACTGGACGATGCATCATCATCTTCAACAAGTGGCCAAGAGGAAGGAATCACACTTGTTAGACGATACGCTGATCCCTGCAAATCACTTTTTCTCTTCACAGTGTTTGTTCTTGTTCTGAGTTCTCTGCTTCTTTTTGTTGAACCTCCACTCAACGCCTTAGCTTCAGACTCTTTTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

420

Amino Acids

45.89

Weight (kDa)

5.36

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 219 - 357 7.8e-33 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 28
AccBSI CCGCTC 1 cut(s) 277
AciI CCGC 1 cut(s) 275
AclI AACGTT 1 cut(s) 444
AclWI GGATC 2 cut(s) 550, 1145
AcoI YGGCCR 1 cut(s) 1111
AcsI RAATTY 2 cut(s) 592, 814
AcuI CTGAAG 1 cut(s) 1227
AfaI GTAC 1 cut(s) 30
AfiI CCNNNNNNNGG 2 cut(s) 38, 851
AgsI TTSAA 6 cut(s) 50, 307, 431, 1079, 1104, 1220
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
Alw21I GWGCWC 1 cut(s) 603
Alw26I GTCTC 3 cut(s) 390, 896, 940
AlwI GGATC 2 cut(s) 550, 1145
AoxI GGCC 2 cut(s) 40, 1111
ApeKI GCWGC 2 cut(s) 190, 1031
ApoI RAATTY 2 cut(s) 592, 814
AseI ATTAAT 1 cut(s) 369
Asp718I GGTACC 1 cut(s) 28
AspS9I GGNCC 4 cut(s) 74, 743, 863, 1001
AsuHPI GGTGA 1 cut(s) 886
AvaII GGWCC 4 cut(s) 74, 743, 863, 1001
AxyI CCTNAGG 1 cut(s) 747
BalI TGGCCA 1 cut(s) 1113
BanI GGYRCC 1 cut(s) 28
BanII GRGCYC 1 cut(s) 603
BbsI GAAGAC 1 cut(s) 1067
Bbv12I GWGCWC 1 cut(s) 603
BbvI GCAGC 2 cut(s) 202, 1018
BcgI CGANNNNNNTGC 4 cut(s) 428, 462, 670, 704
BclI TGATCA 1 cut(s) 645
BcoDI GTCTC 3 cut(s) 390, 896, 940
BfaI CTAG 4 cut(s) 95, 174, 671, 1071
BfmI CTRYAG 2 cut(s) 22, 969
BglII AGATCT 1 cut(s) 510
BisI GCNGC 2 cut(s) 191, 1032
BlsI GCNGC 2 cut(s) 192, 1033
Bme18I GGWCC 4 cut(s) 74, 743, 863, 1001
BmgT120I GGNCC 4 cut(s) 74, 743, 863, 1001
BmiI GGNNCC 3 cut(s) 12, 30, 273
BmsI GCATC 7 cut(s) 101, 160, 173, 319, 346, 1078, 1100
BpiI GAAGAC 1 cut(s) 1067
BplI GAGNNNNNCTC 2 cut(s) 876, 908
BpmI CTGGAG 2 cut(s) 231, 414
Bpu10I CCTNAGC 1 cut(s) 1237
BsaBI GATNNNNATC 2 cut(s) 333, 360
BsaI GGTCTC 1 cut(s) 896
BsaWI WCCGGW 1 cut(s) 13
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 851
Bse1I ACTGG 7 cut(s) 377, 397, 465, 584, 865, 1019, 1086
Bse21I CCTNAGG 1 cut(s) 747
Bse3DI GCAATG 1 cut(s) 552
Bse8I GATNNNNATC 2 cut(s) 333, 360
BseJI GATNNNNATC 2 cut(s) 333, 360
BseLI CCNNNNNNNGG 2 cut(s) 38, 851
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 4 cut(s) 185, 588, 951, 1187
BseNI ACTGG 7 cut(s) 377, 397, 465, 584, 865, 1019, 1086
BseRI GAGGAG 1 cut(s) 326
BseXI GCAGC 2 cut(s) 202, 1018
BsgI GTGCAG 2 cut(s) 674, 775
BshFI GGCC 2 cut(s) 42, 1113
BshNI GGYRCC 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 603
BsiSI CCGG 2 cut(s) 14, 696
BslFI GGGAC 2 cut(s) 163, 741
BslI CCNNNNNNNGG 2 cut(s) 38, 851
BsmAI GTCTC 3 cut(s) 390, 896, 940
BsmFI GGGAC 2 cut(s) 163, 741
BsnI GGCC 2 cut(s) 42, 1113
Bso31I GGTCTC 1 cut(s) 896
Bsp1286I GDGCHC 1 cut(s) 603
Bsp143I GATC 7 cut(s) 292, 510, 517, 542, 645, 786, 1150
BspACI CCGC 1 cut(s) 275
BspANI GGCC 2 cut(s) 42, 1113
BspCNI CTCAG 4 cut(s) 186, 589, 950, 1188
BspLI GGNNCC 3 cut(s) 12, 30, 273
BspPI GGATC 2 cut(s) 550, 1145
BspT107I GGYRCC 1 cut(s) 28
BspTNI GGTCTC 1 cut(s) 896
BsrBI CCGCTC 1 cut(s) 277
BsrDI GCAATG 1 cut(s) 552
BsrI ACTGG 7 cut(s) 377, 397, 465, 584, 865, 1019, 1086
BssMI GATC 7 cut(s) 292, 510, 517, 542, 645, 786, 1150
Bst4CI ACNGT 3 cut(s) 154, 850, 1180
Bst6I CTCTTC 2 cut(s) 615, 1178
BstAPI GCANNNNNTGC 1 cut(s) 170
BstC8I GCNNGC 1 cut(s) 693
BstDEI CTNAG 7 cut(s) 107, 194, 597, 747, 937, 1196, 1237
BstKTI GATC 7 cut(s) 295, 513, 520, 545, 648, 789, 1153
BstMAI GTCTC 3 cut(s) 390, 896, 940
BstMBI GATC 7 cut(s) 292, 510, 517, 542, 645, 786, 1150
BstMWI GCNNNNNNNGC 2 cut(s) 170, 477
BstNSI RCATGY 1 cut(s) 1068
BstSFI CTRYAG 2 cut(s) 22, 969
BstV1I GCAGC 2 cut(s) 202, 1018
BstV2I GAAGAC 1 cut(s) 1067
BstX2I RGATCY 2 cut(s) 510, 542
BstYI RGATCY 2 cut(s) 510, 542
Bsu36I CCTNAGG 1 cut(s) 747
BsuRI GGCC 2 cut(s) 42, 1113
BtsIMutI CAGTG 1 cut(s) 1185
Cac8I GCNNGC 1 cut(s) 693
Cfr13I GGNCC 4 cut(s) 74, 743, 863, 1001
Csp6I GTAC 1 cut(s) 29
CviAII CATG 5 cut(s) 33, 125, 649, 877, 1065
CviQI GTAC 1 cut(s) 29
DdeI CTNAG 7 cut(s) 107, 194, 597, 747, 937, 1196, 1237
DpnI GATC 7 cut(s) 294, 512, 519, 544, 647, 788, 1152
DpnII GATC 7 cut(s) 292, 510, 517, 542, 645, 786, 1150
EaeI YGGCCR 1 cut(s) 1111
Eam1104I CTCTTC 2 cut(s) 615, 1178
EarI CTCTTC 2 cut(s) 615, 1178
Ecl136II GAGCTC 1 cut(s) 601
Eco24I GRGCYC 1 cut(s) 603
Eco31I GGTCTC 1 cut(s) 896
Eco47I GGWCC 4 cut(s) 74, 743, 863, 1001
Eco53kI GAGCTC 1 cut(s) 601
Eco57I CTGAAG 1 cut(s) 1227
Eco81I CCTNAGG 1 cut(s) 747
EcoICRI GAGCTC 1 cut(s) 601
EcoRI GAATTC 1 cut(s) 592
EcoT22I ATGCAT 2 cut(s) 166, 1093
EcoT38I GRGCYC 1 cut(s) 603
FaeI CATG 5 cut(s) 36, 128, 652, 880, 1068
FaqI GGGAC 2 cut(s) 163, 741
FatI CATG 5 cut(s) 32, 124, 648, 876, 1064
FauNDI CATATG 1 cut(s) 1051
FbaI TGATCA 1 cut(s) 645
Fnu4HI GCNGC 2 cut(s) 191, 1032
FriOI GRGCYC 1 cut(s) 603
Fsp4HI GCNGC 2 cut(s) 191, 1032
FspBI CTAG 4 cut(s) 95, 174, 671, 1071
GluI GCNGC 2 cut(s) 191, 1032
GsuI CTGGAG 2 cut(s) 231, 414
HaeIII GGCC 2 cut(s) 42, 1113
HapII CCGG 2 cut(s) 14, 696
Hin1II CATG 5 cut(s) 36, 128, 652, 880, 1068
HinfI GANTC 7 cut(s) 128, 206, 434, 699, 804, 1125, 1247
HpaII CCGG 2 cut(s) 14, 696
HphI GGTGA 1 cut(s) 886
Hpy188III TCNNGA 7 cut(s) 248, 326, 431, 466, 500, 784, 952
HpyAV CCTTC 4 cut(s) 301, 949, 991, 1115
HpyCH4III ACNGT 3 cut(s) 154, 850, 1180
HpyCH4IV ACGT 1 cut(s) 444
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 477
HpyF3I CTNAG 7 cut(s) 107, 194, 597, 747, 937, 1196, 1237
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 5 cut(s) 36, 128, 652, 880, 1068
KpnI GGTACC 1 cut(s) 32
Ksp22I TGATCA 1 cut(s) 645
Kzo9I GATC 7 cut(s) 292, 510, 517, 542, 645, 786, 1150
LmnI GCTCC 4 cut(s) 16, 626, 884, 1028
Lsp1109I GCAGC 2 cut(s) 202, 1018
LweI GCATC 7 cut(s) 101, 160, 173, 319, 346, 1078, 1100
MaeI CTAG 4 cut(s) 95, 174, 671, 1071
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 2 cut(s) 673, 733
MalI GATC 7 cut(s) 294, 512, 519, 544, 647, 788, 1152
MbiI CCGCTC 1 cut(s) 277
MboI GATC 7 cut(s) 292, 510, 517, 542, 645, 786, 1150
MboII GAAGA 6 cut(s) 365, 602, 821, 1067, 1092, 1165
MflI RGATCY 2 cut(s) 510, 542
MhlI GDGCHC 1 cut(s) 603
MlsI TGGCCA 1 cut(s) 1113
MluCI AATT 2 cut(s) 592, 814
MluNI TGGCCA 1 cut(s) 1113
MlyI GAGTC 2 cut(s) 693, 1241
MmeI TCCRAC 2 cut(s) 297, 634
Mox20I TGGCCA 1 cut(s) 1113
Mph1103I ATGCAT 2 cut(s) 166, 1093
MscI TGGCCA 1 cut(s) 1113
MseI TTAA 3 cut(s) 348, 369, 588
MslI CAYNNNNRTG 1 cut(s) 680
Msp20I TGGCCA 1 cut(s) 1113
MspA1I CMGCKG 2 cut(s) 193, 485
MspI CCGG 2 cut(s) 14, 696
MwoI GCNNNNNNNGC 2 cut(s) 170, 477
NdeI CATATG 1 cut(s) 1051
NdeII GATC 7 cut(s) 292, 510, 517, 542, 645, 786, 1150
NlaIII CATG 5 cut(s) 36, 128, 652, 880, 1068
NlaIV GGNNCC 3 cut(s) 12, 30, 273
NmeAIII GCCGAG 1 cut(s) 475
NmuCI GTSAC 1 cut(s) 673
NsiI ATGCAT 2 cut(s) 166, 1093
NspI RCATGY 1 cut(s) 1068
PfeI GAWTC 5 cut(s) 128, 206, 434, 804, 1125
PkrI GCNGC 2 cut(s) 192, 1033
PleI GAGTC 2 cut(s) 693, 1241
PpsI GAGTC 2 cut(s) 693, 1241
PshBI ATTAAT 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 603
Psp1406I AACGTT 1 cut(s) 444
PspN4I GGNNCC 3 cut(s) 12, 30, 273
PspPI GGNCC 4 cut(s) 74, 743, 863, 1001
PsuI RGATCY 2 cut(s) 510, 542
PvuII CAGCTG 2 cut(s) 193, 485
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
RseI CAYNNNNRTG 1 cut(s) 680
SacI GAGCTC 1 cut(s) 603
SaqAI TTAA 3 cut(s) 348, 369, 588
SatI GCNGC 2 cut(s) 191, 1032
Sau3AI GATC 7 cut(s) 292, 510, 517, 542, 645, 786, 1150
Sau96I GGNCC 4 cut(s) 74, 743, 863, 1001
SchI GAGTC 2 cut(s) 693, 1241
SduI GDGCHC 1 cut(s) 603
SfaNI GCATC 7 cut(s) 101, 160, 173, 319, 346, 1078, 1100
SfcI CTRYAG 2 cut(s) 22, 969
SinI GGWCC 4 cut(s) 74, 743, 863, 1001
SmiMI CAYNNNNRTG 1 cut(s) 680
Sse9I AATT 2 cut(s) 592, 814
SsiI CCGC 1 cut(s) 275
SspMI CTAG 4 cut(s) 95, 174, 671, 1071
SstI GAGCTC 1 cut(s) 603
TaaI ACNGT 3 cut(s) 154, 850, 1180
TaiI ACGT 1 cut(s) 447
TaqII GACCGA 1 cut(s) 880
TasI AATT 2 cut(s) 592, 814
TfiI GAWTC 5 cut(s) 128, 206, 434, 804, 1125
Tru1I TTAA 3 cut(s) 348, 369, 588
Tru9I TTAA 3 cut(s) 348, 369, 588
TscAI CASTG 1 cut(s) 1185
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 2 cut(s) 190, 1031
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 4 cut(s) 72, 366, 695, 759
TspRI CASTG 1 cut(s) 1185
VpaK11BI GGWCC 4 cut(s) 74, 743, 863, 1001
VspI ATTAAT 1 cut(s) 369
XapI RAATTY 2 cut(s) 592, 814
XceI RCATGY 1 cut(s) 1068
XspI CTAG 4 cut(s) 95, 174, 671, 1071
Zsp2I ATGCAT 2 cut(s) 166, 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.